CN219790931U - Kit - Google Patents

Abstract

The utility model relates to a kit comprising: the box body and the mounting frame body are detachably connected with the box body; the box body comprises a plurality of mutually connected reagent strips, and each reagent strip is provided with a plurality of functional hole sites; be equipped with a plurality of confession reagent strip male reagent strip installation position on the mounting bracket body, still be equipped with spacing fixed subassembly on the mounting bracket body of length direction along reagent strip installation position, spacing fixed subassembly is used for fixing the reagent strip after the reagent strip is installed the reagent strip installation position. The utility model adopts the reagent sealing film, so that pollution can not occur, and the product precision is improved. Meanwhile, the detachable installation of the reagent strip and the installation frame body can be realized, the installation of the reagent strip is more stable, and the pressing and fixing of the installation frame body can prevent the reagent strip from shaking and splashing the reagent.

Description

Kit

Technical Field

The utility model relates to the technical field of kits, in particular to a kit.

Background

Nucleic acid is a kind of biological polymer, the most important substance in all biological molecules, and exists in all animal and plant cells and microorganisms widely. In various medical research fields, nucleic acid extraction means are often used to analyze and detect corresponding detection substances, and the key to preserving the nucleic acid extraction substances is to use a kit for transfer and reaction.

When the existing kit is used, the sealing film treatment is not performed, the reagent is manually added into the reagent strip on the spot, and the pollution is easily caused in the process of manually adding the reagent; generally set up flip on the kit consumptive material, but increased the cost that flip will increase the consumptive material, need open flip moreover during the use, more a motion of opening flip has reduced the degree of nucleic acid extraction automation. If the kit is to take reagent transportation, flip setting is easy in taking reagent transportation, makes the consumptive material splash, and the installation is unstable.

Disclosure of Invention

The utility model aims to provide a kit, which can prevent a reagent sealing film from being polluted and improve the product precision. Meanwhile, the detachable installation of the reagent strip and the installation frame body can be realized, the installation of the reagent strip is more stable, and the pressing and fixing of the installation frame body can prevent the reagent strip from shaking and splashing the reagent.

Embodiments of the present utility model are implemented as follows:

the utility model provides a kit comprising: the box body and the mounting frame body are detachably connected with the box body; the kit body comprises a plurality of mutually connected reagent strips, and each reagent strip is provided with a plurality of functional hole sites; be equipped with a plurality of confession on the mounting bracket body reagent strip male reagent strip installation position, follow the length direction of reagent strip installation position still be equipped with spacing fixed subassembly on the mounting bracket body, spacing fixed subassembly is used for work as the reagent strip is installed after the reagent strip installation position, fix the reagent strip.

In the above technical scheme, because the box body is disposable consumable, need renew after using, consequently, the box body can be dismantled with the mounting bracket body, makes things convenient for the timely change of box body. Adopt the mounting bracket body to fix the box body to make the experimental process more stable.

In one embodiment, the mounting frame body comprises a bottom plate, a vertical plate and a partition plate; the bottom plate is connected with the vertical plate, the partition plates are arranged on the vertical plate, the bottoms of the partition plates are connected with the bottom plate, and the reagent strip mounting positions are formed between the adjacent partition plates; and the limiting and fixing assembly is arranged on the partition board.

In the technical scheme, the bottom plate is used for supporting the whole installation frame body, the vertical plate is used for supporting the partition plate, the partition plate separates the reagent strips, and the reagent strips are fixed between limiting and fixing.

In one embodiment, the riser comprises: the device comprises a first vertical plate, a second vertical plate, a third vertical plate and a fourth vertical plate, wherein the first vertical plate, the second vertical plate, the third vertical plate and the fourth vertical plate are sequentially connected with each other to form a closed loop, the first vertical plate is opposite to the third vertical plate, and the second vertical plate is opposite to the fourth vertical plate.

In the above technical scheme, the first vertical plate, the second vertical plate, the third vertical plate and the fourth vertical plate are mutually connected to form a rectangular structure of the whole vertical plate. The first vertical plate, the second vertical plate, the third vertical plate and the fourth vertical plate can be detachably connected.

In an embodiment, the limiting and fixing assembly comprises a first limiting pressing bar and a second limiting pressing bar, wherein the first limiting pressing bar is arranged at one end part of the partition board, and the second limiting pressing bar is arranged at the other end part of the partition board; the second vertical plate is provided with a plurality of first limit grooves, the fourth vertical plate is provided with a plurality of second limit grooves, one end part of the partition plate provided with the first limit pressing strip is arranged in the first limit grooves, and the other end part of the partition plate provided with the second limit pressing strip is arranged in the second limit grooves.

In the above technical scheme, the first limiting grooves are distributed on the second vertical plate at equal intervals, and the second limiting grooves are distributed on the second vertical plate at equal intervals. The two ends of the partition boards are respectively embedded and installed in the first limiting groove of the second vertical board and the second limiting groove of the fourth vertical board, and each partition board can be spaced apart by adopting the embedded installation mode, so that a reagent strip installation position for placing a functional hole site on a reagent strip is formed between the adjacent partition boards.

In an embodiment, the second vertical plate is provided with an elastic pin one, and one end of the reagent strip is correspondingly provided with a locking hole.

In the above technical scheme, in order to strengthen the stability that the reagent strip is fixed and pressed in the riser, through setting up the elasticity pin one on the second riser, correspond to setting up the locking hole on one of them end of reagent strip. Specifically, the locking hole is provided at one end of the reagent strip near the elution hole. When one end of the reagent strip close to the elution hole moves along the first guide surface below the first limiting pressing strips and is pushed into and pressed into the adjacent two first limiting pressing strips, the locking hole on the reagent strip can be correspondingly pressed into the elastic pin I on the second vertical plate, and the elastic pin I is recovered to be sprung under the elastic action, so that the locking hole can cover the elastic pin I, and one end of the reagent strip is stably pressed into the second vertical plate.

In an embodiment, the fourth vertical plate is provided with an elastic pin II, and the other end of the reagent strip is correspondingly provided with a in-place limiting hole.

In the above technical scheme, in order to strengthen the stability that the reagent strip is fixed to be compressed tightly in the riser more, through be equipped with elasticity pin two on the fourth riser, be equipped with the spacing hole in place on the other end of reagent strip correspondingly. Specifically, the in-place limiting hole is arranged at one end of the reagent strip close to the cracking hole. When one end of the reagent strip close to the elution hole moves along the first guide surface below the first limiting pressing strips and is pushed into the two adjacent first limiting pressing strips, the other end of the reagent strip close to the cracking hole is pushed into the two adjacent second limiting pressing strips, at the moment, the locking hole on the reagent strip can be correspondingly pressed into the first elastic pin on the second vertical plate, the first elastic pin is sprung under the elastic action, the locking hole can cover the first elastic pin, so that one end of the reagent strip is stably pressed into the second vertical plate, meanwhile, the in-place limiting hole on the other end of the reagent strip can be correspondingly pressed into the second elastic pin on the fourth vertical plate, the second elastic pin is sprung back under the elastic action, and the second elastic pin can be sleeved by the in-place limiting hole, so that the other end of the reagent strip is stably pressed into the fourth vertical plate.

In an embodiment, the first vertical plate is provided with a first handle, and the third vertical plate is provided with a second handle.

In the technical scheme, the first handle and the second handle are arranged to facilitate the extraction and placement of the mounting frame body.

In an embodiment, a protruding portion is disposed at an end of the reagent strip, and the protruding portion is used for performing secondary limiting and locking after the limiting and fixing component limits and fixes the reagent strip.

In the above technical solution, the protruding portion is provided on one end of the reagent strip near the cleavage hole, so that the width of the reagent strip on the one end is larger than the width of the other end of the reagent strip. One end of the reagent strip close to the elution hole moves along the first guide surface below the first limiting pressing strips and is pushed into and pressed into the two adjacent first limiting pressing strips, the other end of the reagent strip close to the cracking hole is pushed into the two adjacent second limiting pressing strips, and the locking Kong Taozhu elastic pin on the reagent strip is a position limiting Kong Taozhu elastic pin II on the other end of the reagent strip, so that the two adjacent second limiting pressing strips can abut against a limiting space formed by the protruding parts on the adjacent reagent strip.

In one embodiment, the reagent strip is provided with a membrane cutting groove.

In the technical scheme, the universal film can be used for sealing the film on the box body, so that the cost is low, the efficiency is higher, and pollution is avoided.

In one embodiment, the reagent strip is further provided with a sealing membrane reinforcing rib.

In the technical scheme, the sealing membrane reinforcing ribs are arranged on the reagent strips, so that the sealing performance of the membrane can be further improved.

In an embodiment, the functional well sites include an elution well, a wash well, a conversion well, and a lysis well, the lysis well having a volume greater than the volume of the elution well, the wash well, and the conversion well.

In the technical scheme, the reagent strip can realize the treatment of a large-capacity sample.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the overall structure of a kit according to an embodiment of the present utility model;

FIG. 2 is a top view of a kit according to an embodiment of the present utility model;

FIG. 3 is a schematic structural view of a reagent strip according to a first embodiment of the present utility model;

FIG. 4 is a schematic structural view of a reagent strip according to a second embodiment of the present utility model;

fig. 5 is a schematic structural diagram of a mounting frame according to an embodiment of the present utility model;

fig. 6 is a schematic structural diagram of a mounting frame according to a second embodiment of the present utility model;

FIG. 7 is a top view of a mounting frame according to an embodiment of the present utility model;

fig. 8 is a schematic installation diagram of a box body and a mounting frame body according to an embodiment of the utility model.

Icon:

1-a kit; 100-box body; 110-reagent strips; 111-locking holes; 112-in-place limiting holes; 113-a boss; 114-sealing film reinforcing ribs; 120-functional hole sites; 121-elution well; 122-wash wells; 123-switching holes; 124-lysis wells; 130-cutting a film groove; 200-mounting frame body; 210-a bottom plate; 220-vertical plates; 221-a first riser; 2211—a first handle; 222-a second riser; 2221—a first limit groove; 2222-elastic pin one; 223-a third riser; 2231-a second handle; 224-fourth riser; 2241-a second limit groove; 2242-elastic pin two; 230-a separator; 240-reagent strip mounting location; 250-limiting and fixing components; 251-a first limit pressing bar; 2511—a first guide surface; 252-a second limit pressing bar; 2521-a second guide surface; 300-membrane.

Detailed Description

The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc., are based on directions or positional relationships shown in the drawings, or directions or positional relationships conventionally put in use of the product of the application, are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "coupled" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.

The technical scheme of the utility model will be described with reference to the accompanying drawings.

Fig. 1 and fig. 2 are schematic views of the overall structure of a kit 1 according to an embodiment of the utility model. A kit 1 comprising: the box body 100 and the mounting frame body 200 which is detachably connected with the box body 100. The cartridge 100 includes a plurality of interconnected reagent strips 110, and each reagent strip 110 has a plurality of functional holes 120. Because the box body 100 is disposable, the box body 100 needs to be replaced after being used, and therefore, the box body 100 and the mounting frame body 200 can be detached, and the box body 100 can be replaced in time conveniently.

The reagent strip 110 is mainly used for sample processing and product extraction. In some product extraction processes, a larger volume of reagent strip 110 is required to meet the product extraction requirements due to the larger amount of reagent. Referring to fig. 3, which is a schematic diagram of a structure of a reagent strip 110 according to a first embodiment of the present utility model, the reagent strip 110 in fig. 3 can be used for processing large sample volumes. The functional well sites 120 on the reagent strip 110 include an elution well 121, a wash well 122, a switch well 123, and a lysis well 124. The volumetric volume of the lysis wells 124 is greater than the volumetric volumes of the elution wells 121, wash wells 122, and switch wells 123. Wherein the bottom of the elution well 121 is sharp so that the same amount of reagent can be given a higher height and the bottom of the elution well 121 can be used for heating. The washing holes 122 are used for storing washing liquid or magnetic beads, and the washing holes 122 can be provided with a plurality of holes and can support function expansion. The switching hole 123 is a hole site for switching the magnetic rod sleeve and the suction head, and the cracking hole 124 is used for cracking reaction of large sample volume, such as 8ml, 15ml and the like; the lysing holes 124 are shaped as bar shaped holes, the bottoms of which can be used for heating. The volume of the elution well 121, the washing well 122, and the conversion well 123 may be 1ml, 2ml, or the like, and the elution well 121, the washing well 122, and the conversion well 123 are circular wells or elliptical wells.

The samples in all the functional hole sites 120 can be mixed in a suction head mixing mode, the conversion hole 123 and the cracking hole 124 use magnetic rods sleeved with magnetic rods to conduct magnetic attraction, the magnetic attraction time can be greatly reduced by the magnetic rod sleeved with magnetic attraction, and the elution hole 121 and the washing hole 122 can use suction heads to attract magnetism or the magnetic rod sleeved with magnetic rods to attract magnetism. When the suction head is mixed, the magnet is not close to the suction head, and the pipette carries out reagent handling so as to mix the reagents; when the suction head sucks magnetism, the magnet is close to the suction head, and the pipette is used for handling reagents, so that magnetic beads can be adsorbed on the side wall of the suction head.

In the embodiment shown in FIG. 3, it is preferable that the number of elution wells 121 is 1, the number of washing wells 122 is 8, the number of conversion wells 123 is 1, the number of lysis wells 124 is 1, and the amount of sample that can be processed by the lysis wells 124 is 8ml at the maximum.

Since small product extraction is also typically involved in nucleic acid extraction, smaller volumes of reagent strips 110 are often required to avoid wastage of consumables. Referring to fig. 4, which is a schematic diagram of a structure of a reagent strip 110 according to a second embodiment of the present utility model, the reagent strip 110 in fig. 4 can be used for processing small sample volumes. The functional well 120 on the reagent strip 110 in FIG. 4 includes an elution well 121, a wash well 122, and a lysis well 124. Preferably, the number of elution wells 121 can be set to 2 for multiple elution requirements of a sample, the number of washing wells 122 to 8, the number of lysis wells 124 to 2, and the round Kong Liejie wells 124 of the lysis wells 124 can be used for lysis reactions of small samples, e.g., 2ml. The volume of the elution well 121 and the washing well 122 can be 1ml or 2ml, and the elution well 121 and the washing well 122 are circular wells.

Because the processing liquid such as the washing liquid, the eluent, the lysate, etc. is pre-placed in the functional hole site 120, in order to avoid the processing liquid in the functional hole site 120 from being polluted before the use of the kit 1, the reagent strip 110 may be subjected to the film sealing treatment in advance. Referring to fig. 2 and 3, a film 300 is integrally adhered to the case 100, so that the whole film 300 covers all the reagent strips 110 for heat shrinkage sealing, and then the reagent strips 110 are individually sealed by cutting along the film cutting position 1 shown in fig. 1 by a film cutting instrument.

However, since the hole position of the cracking hole 124 in fig. 3 is larger than a single round hole, when the suction head or the magnetic rod sleeve enters the cracking hole 124 to mix, the left and right movement is required, and the film puncturing mechanism only supports to puncture round holes such as the elution hole 121, the washing hole 122 and the conversion hole 123 when performing film puncturing operation, so that the strip-shaped cracking hole 124 cannot be effectively punctured. Therefore, the membrane cutting groove 130 can be additionally arranged on the reagent strip 110, the membrane cutting groove 130 is positioned between the cracking hole 124 and the conversion hole 123, the membrane cutting instrument is used for cutting along the membrane cutting position 2 (namely the membrane cutting groove 130) shown in fig. 1, the whole membrane 300 is cut into two parts, one part is sealed on the elution hole 121, the washing hole 122 and the conversion hole 123, the other part is sealed on the cracking hole 124, and when the cracking hole 124 needs to be used, the part of the membrane 300 can be manually torn off.

In another embodiment, a piece of film 300 may be adhered to the case 100, and the film cutting apparatus is used to cut along the film cutting position 2 (i.e. the film cutting slot 130) shown in fig. 1, so that the surface of the area where the elution hole 121, the washing hole 122 and the conversion hole 123 are located is sealed, and after the redundant film 300 is cut, the surface of the cleavage hole 124 is not sealed.

The manner of sealing the reagent strip 110 shown in fig. 4 is the same as that described above for the reagent strip 110 of fig. 3, and will not be described again here. The difference is that the reagent strip 110 shown in fig. 4 is not provided with the film cutting groove 130, because the elution hole 121, the washing hole 122 and the cleavage hole 124 in fig. 4 are all circular holes, and the film punching operation can be successfully completed by adopting the film punching mechanism without manual film tearing.

In order to further improve the sealing performance of the membrane 300, referring to fig. 3, the reagent strip 110 is provided with two membrane sealing ribs 114, preferably two membrane sealing ribs 114 are respectively provided at two ends of the reagent strip 110, one of which is disposed beside the cleavage hole 124, and the other of which is disposed beside the elution hole 121. In the above embodiment, the overall film sealing process is performed on the case 100.

Referring to FIG. 4, two membrane seal ribs 114 are provided on the reagent strip 110, one of which is disposed beside the elution well 121 and the other of which is disposed between the lysis well 124 and the washing well 122.

Referring to fig. 5, 6 and 7, a plurality of reagent strip mounting positions 240 for inserting the reagent strips 110 are provided on the mounting frame 200, and a limiting and fixing assembly 250 is further provided on the mounting frame 200 along the length direction of the reagent strip mounting positions 240, wherein the limiting and fixing assembly 250 is used for fixing the reagent strips 110 after the reagent strips 110 are mounted on the reagent strip mounting positions 240.

In one embodiment, the mounting frame 200 may include a bottom plate 210, a vertical plate 220, and a partition plate 230, wherein the bottom plate 210 is connected to the vertical plate 220, the partition plate 230 is disposed on the vertical plate 220, the bottom of the partition plate 230 is connected to the bottom plate 210, and a reagent strip mounting position 240 is formed between adjacent partition plates. The spacing fixing assembly 250 is disposed on the partition. Wherein the bottom plate 210 is used for supporting the whole mounting frame body 200, the vertical plate 220 is used for supporting the partition plate 230, and the partition plate 230 separates the reagent strips 110.

Further, in order to achieve the detachable connection of the riser 220 with the partition 230, the riser 220 may include: the first vertical plate 221, the second vertical plate 222, the third vertical plate 223 and the fourth vertical plate 224 are sequentially connected with each other to form a closed loop, wherein the first vertical plate 221 is opposite to the third vertical plate 223, and the second vertical plate 222 is opposite to the fourth vertical plate 224.

In this embodiment, referring to fig. 6, it is preferable that the first upright plate 221, the second upright plate 222, the third upright plate 223, and the fourth upright plate 224 are connected to each other to form a rectangular structure of the whole of the upright plate 220. The first vertical plate 221, the second vertical plate 222, the third vertical plate 223, and the fourth vertical plate 224 may be connected to each other in a detachable connection direction such as a snap connection or a bolt connection.

Referring to fig. 5, the limiting and fixing assembly 250 includes a first limiting bead 251 and a second limiting bead 252, the first limiting bead 251 is disposed at one end of the partition 230, and the second limiting bead 252 is disposed at the other end of the partition 230.

Referring to fig. 6 and 7, a plurality of first limiting grooves 2221 are formed on the second vertical plate 222, a plurality of second limiting grooves 2241 are formed on the fourth vertical plate 224, one end of the partition 230 provided with the first limiting bead 251 is mounted in the first limiting groove 2221, and the other end of the partition 230 provided with the second limiting bead 252 is mounted in the second limiting groove 2241.

In this embodiment, the first limit grooves 2221 are equally spaced on the second riser 222, and the second limit grooves 2241 are equally spaced on the fourth riser 224. The two ends of the partition plates 230 are respectively inserted and mounted in the first limit grooves 2221 of the second riser 222 and the second limit grooves 2241 of the fourth riser 224, so that each partition plate 230 can be spaced apart by the insertion and mounting, thereby forming the reagent strip mounting sites 240 for placing the functional hole sites 120 on the reagent strips 110 between the adjacent partition plates 230. It should be noted that, the spacing between the adjacent first limit grooves 2221 and the spacing between the adjacent second limit grooves 2241 may be adjusted according to the width of the reagent strip 110. In this embodiment, in order to clearly show the length and width of the mounting frame body 200, the length direction of the first vertical plate 221 and the third vertical plate 223 is taken as the length direction of the mounting frame body 200, and the length direction of the second vertical plate 222 and the fourth vertical plate 224 is taken as the width direction of the mounting frame body 200, which is not a limitation of the present utility model.

Referring to fig. 2 and 8, as shown in fig. 8 (a), when the cartridge 100 is mounted on the mounting frame 200, each reagent strip 110 on the cartridge 100 is placed down along the reagent strip mounting position 240 toward the bottom plate 210.

As shown in fig. 2 and 8 (b), when the reagent strip 110 is lowered to the positions where the two ends reach the first limiting bead 251 and the second limiting bead 252, respectively, one end of the reagent strip 110 near the elution hole 121 is pushed under the adjacent two first limiting beads 251, and the other end of the reagent strip 110 near the cleavage hole 124 is pushed under the adjacent two second limiting beads 252, and after both the left and right ends of the reagent strip 110 are pushed in place, the reagent strip 110 is shown to be mounted in place. At this time, the reagent strip 110 is smoothly mounted and pressed into the vertical plate 220 defined by the first vertical plate 221, the second vertical plate 222, the third vertical plate 223 and the fourth vertical plate 224 under the simultaneous limit fixing action of the first limit pressing bar 251 and the second limit pressing bar 252, as shown in fig. 2 and 8 (c).

Further, in order to enable the two ends of the reagent strip 110 to smoothly enter under the first limit bead 251 and the second limit bead 252, in this embodiment, an inclined first guide surface 2511 is provided on the surface of the first limit bead 251, and an inclined second guide surface 2521 is provided on the surface of the second limit bead 252. In this way, one end of the reagent strip 110 near the elution hole 121 is pushed under the two adjacent first limiting beads 251, the other end of the reagent strip 110 near the cleavage hole 124 is pushed under the two adjacent second limiting beads 252, and the reagent strip 110 can smoothly enter the vertical plate 220 by the guiding action of the inclined first guiding surface 2511 and the second guiding surface 2521, so that the reagent strip is pressed and fixed by the vertical plate 220. That is, the first guide surface 2511 and the second guide surface 2521 are both used to enhance the guiding of the vertical plate 220 and the reagent strip 110 when lowered.

Optionally, in order to enhance the stability of the reagent strip 110 being fixedly pressed in the vertical plate 220, in this embodiment, referring to fig. 3 and 7, an elastic pin 2222 is provided on the second vertical plate 222, and a locking hole 111 is correspondingly provided on one end of the reagent strip 110 (referring to fig. 3). Specifically, the locking aperture 111 is provided on one end of the reagent strip 110 near the elution aperture 121. When one end of the reagent strip 110 near the elution hole 121 is moved along the first guiding surface 2511 to the lower side of the first limiting bead 251 and pushed into the two adjacent first limiting beads 251 to be pressed into the elastic pins 2222 on the second vertical plate 222, the elastic pins 2222 are recovered to spring up under the elastic action, so that the elastic pins 2222 can be sleeved by the locking holes 111, and one end of the reagent strip 110 is stably pressed into the second vertical plate 222.

Further, in order to further enhance the stability of the reagent strip 110 being fixed and pressed in the vertical plate 220, referring to fig. 3 and 7, based on the above embodiment, the elastic pin two 2242 is provided on the fourth vertical plate 224, and the in-place limiting hole 112 is correspondingly provided on the other end of the reagent strip 110 (referring to fig. 3). Specifically, the in-place limiting aperture 112 is disposed on one end of the reagent strip 110 adjacent to the lysis aperture 124. When one end of the reagent strip 110 near the elution hole 121 is moved along the first guiding surface 2511 to the lower side of the first limiting bead 251 and pushed into the two adjacent first limiting beads 251 to be pressed into the two adjacent second limiting beads 252, at this time, the locking hole 111 on the reagent strip 110 can be correspondingly pressed into the elastic pin one 2222 on the second vertical plate 222, the elastic pin one 2222 is recovered to spring under the elastic action, so that the locking hole 111 can cover the elastic pin one 2222, thereby stably pressing one end of the reagent strip 110 into the second vertical plate 222, and at the same time, the in-place limiting hole 112 on the other end of the reagent strip 110 can be correspondingly pressed into the elastic pin two 2242 on the fourth vertical plate 224, the elastic pin two 2242 is recovered to spring under the elastic action, so that the obtained in-place limiting hole 112 can cover the elastic pin two 2242, thereby stably pressing the other end of the reagent strip 110 into the fourth vertical plate 224.

In an embodiment, referring to fig. 3, a protruding portion 113 is disposed at an end of the reagent strip 110, and the protruding portion 113 is used for performing secondary limit locking after the limit fixing assembly 250 performs limit and fixing on the reagent strip 110.

Preferably, in the above embodiment, the protrusion 113 is provided on one end of the reagent strip 110 near the cleavage hole 124 such that the width of the reagent strip 110 on the one end is greater than the width of the other end of the reagent strip 110. As shown in fig. 8 (b), one end of the reagent strip 110 near the elution hole 121 is moved along the first guiding surface 2511 to the lower side of the first limiting bead 251 and pushed into and pressed into the adjacent two first limiting beads 251, the other end of the reagent strip 110 near the cleavage hole 124 is pushed into the adjacent two second limiting beads 252, the locking hole 111 on the reagent strip 110 is sleeved on the elastic pin one 2222, and the in-place limiting hole 112 on the other end of the reagent strip 110 is sleeved on the elastic pin two 2242, so that the adjacent two second limiting beads 252 can abut against the limiting space formed by the protruding part 113 on the adjacent reagent strip 110, as shown in fig. 2.

Further, in order to facilitate the extraction and placement of the mounting frame body 200, in the embodiment shown in fig. 1, 2, and 5-8, a first handle 2211 is provided on the first vertical plate 221, and a second handle 2231 is provided on the third vertical plate 223.

In summary, the kit 1 of the present utility model can realize the detachable installation of the reagent strip 110 and the mounting frame body 200, the installation of the reagent strip 110 is more stable, and the compacting and fixing of the mounting frame body 200 can prevent the reagent strip 110 from shaking and splashing the reagent. Second, the film sealing method of the present utility model prevents the reagent in the reagent strip 110 from being polluted, thereby improving the product accuracy.

Referring to fig. 3, the sample processing process performed by using one of the reagent strips 110 in the cartridge 100 according to the present utility model is as follows:

defining a lysis well 124 as 1 well, in which a sample, a lysis solution, a binding solution, magnetic beads, DNase, etc. can be contained, a reagent amount is 5ml, a conversion well 123 is 2 wells, in which a washing solution 1 can be contained, a reagent amount is 1ml, a washing well 122 is 3 wells, in which a washing solution 1 can be contained, a reagent amount is 1ml, the washing well 122 is 4 wells, 5 wells, 6 wells, 7 wells, 8 wells, 9 wells, in which 4 wells, 5 wells, 6 wells are used for storing the washing solution 2, a reagent amount is 1ml,7 wells, 8 wells, 9 wells are used for more washing needs, and in which a reaction reagent can be stored; the elution well 121 is 10 wells and may contain TE buffer therein, in an amount of 50uL.

The first step: cracking temperature control is carried out, heating is carried out by starting a heating module, the heating temperature is 65 ℃ and the temperature is kept constant for 10min, the suction head moves left and right in 1 hole (cracking hole 124, the same applies below), meanwhile, liquid suction and injection actions are carried out, and full cracking occurs in 1 hole.

And a second step of: the binding reaction was carried out by heating at 30℃for 5 minutes while the tip was moved to the left and right in 1 well and simultaneously performing pipetting, whereby 1. 1 Kong Cizhu was sufficiently bound to nucleic acid.

And a third step of: the magnetic beads are adsorbed, the parts of the magnetic rod sleeve and the magnet combination are replaced, the temperature control is not carried out, the magnetic rod and the magnetic rod sleeve combination piece moves left and right in a segmented mode at the 1 hole, and finally the magnetic beads are adsorbed to the bottom of the magnetic rod sleeve.

Fourth step: and the magnetic beads are transferred, the magnetic rod and magnetic rod sleeve assembly is transferred to 2 holes (the switching holes 123 are the same as below), meanwhile, the magnetic rod is separated from the magnetic rod sleeve, only the residual magnetic rod is sleeved in the 2 holes, and the magnetic rod sleeve vibrates up and down circularly at the moment, so that the magnetic beads fall off from the magnetic rod sleeve to the 2 holes.

Fifth step: washing the magnetic beads, replacing the suction head to the 2 holes, and circularly sucking and injecting liquid by the suction head, so that the washing liquid in the 2 holes is fully mixed with the magnetic beads, the proteins adsorbed on the magnetic beads are sucked, and the nucleic acid is continuously adsorbed by the magnetic beads.

If necessary for the test, washing may be performed in 7-well, 8-well, or 9-well.

Sixth step: and (3) transferring the magnetic beads for the second time, controlling the horizontal magnet to be close to the suction head, continuously sucking and injecting the liquid reagent by the suction head, and finally discharging all the reagent into the suction head. Due to the action of the magnet, the beads are all attracted to the tip sidewall, at which point the tip is replaced with 3 wells (wash wells 122).

Seventh step: and (3) repeating the washing steps, repeating the steps of the fifth step and the sixth step, enabling the magnetic beads to pass through the washing holes (122), the washing holes (122) and the washing holes (122) again, enabling the magnetic beads to be purer, and finally enabling the suction head with the magnetic beads to stay in the holes (10) (elution holes 121).

Eighth step: eluting, namely, fully mixing the eluent in the 10 holes with the magnetic beads through the action of sucking liquid and injecting liquid by the suction head in a circulating way, and sucking pure nucleic acid adsorbed on the magnetic beads by the eluent.

Ninth step: discarding the magnetism, controlling the horizontal magnet to be close to the suction head, continuously sucking and injecting the liquid reagent by the suction head, and discharging all the reagent into the suction head. Because of the effect of the magnet, the magnetic beads are all adsorbed on the side wall of the suction head, the suction head is replaced to other positions, and finally the reagent with the nucleic acid substance is obtained in the 10 # hole.

It should be noted that the features of the embodiments of the present utility model may be combined with each other without conflict.

The above is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (8)

1. A kit, comprising: the box body and the mounting frame body are detachably connected with the box body;

the kit body comprises a plurality of mutually connected reagent strips, and each reagent strip is provided with a plurality of functional hole sites;

the mounting frame body is provided with a plurality of reagent strip mounting positions for the insertion of the reagent strips, the mounting frame body along the length direction of the reagent strip mounting positions is also provided with a limiting and fixing assembly, and the limiting and fixing assembly is used for fixing the reagent strips after the reagent strips are mounted on the reagent strip mounting positions;

the mounting frame body comprises a bottom plate, a vertical plate and a partition plate;

the bottom plate is connected with the vertical plate, the partition plates are arranged on the vertical plate, the bottoms of the partition plates are connected with the bottom plate, and the reagent strip mounting positions are formed between the adjacent partition plates;

the limiting and fixing assembly is arranged on the partition board;

the riser includes:

the device comprises a first vertical plate, a second vertical plate, a third vertical plate and a fourth vertical plate, wherein the first vertical plate, the second vertical plate, the third vertical plate and the fourth vertical plate are sequentially connected with one another to form a closed loop, the first vertical plate is opposite to the third vertical plate, and the second vertical plate is opposite to the fourth vertical plate;

the limiting and fixing assembly comprises a first limiting pressing strip and a second limiting pressing strip, the first limiting pressing strip is arranged at one end part of the partition board, and the second limiting pressing strip is arranged at the other end part of the partition board;

the second vertical plate is provided with a plurality of first limit grooves, the fourth vertical plate is provided with a plurality of second limit grooves, one end part of the partition plate provided with the first limit pressing strip is arranged in the first limit grooves, and the other end part of the partition plate provided with the second limit pressing strip is arranged in the second limit grooves.

2. The kit of claim 1, wherein the second vertical plate is provided with an elastic pin one, and one end of the reagent strip is correspondingly provided with a locking hole.

3. The kit according to claim 2, wherein the fourth vertical plate is provided with an elastic pin II, and the other end of the reagent strip is correspondingly provided with a position limiting hole.

4. The kit of claim 1, wherein the first riser is provided with a first handle and the third riser is provided with a second handle.

5. A kit according to claim 1 or 3, wherein a protruding portion is provided at one end of the reagent strip, and the protruding portion is used for performing secondary limit locking after the limit fixing component performs limit and fixing on the reagent strip.

6. The kit of claim 1, wherein the reagent strip is provided with a membrane cutting groove.

7. The kit of claim 1, wherein the reagent strip is further provided with a sealing membrane reinforcing rib.

8. The kit of claim 1, wherein the functional well sites comprise an elution well, a wash well, a switch well, and a lysis well, the lysis well having a volumetric volume greater than the volumetric volumes of the elution well, the wash well, and the switch well.