CN215678416U - Multi-channel test strip determination plate compatible with fluorescent microplate reader - Google Patents

Multi-channel test strip determination plate compatible with fluorescent microplate reader Download PDF

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Publication number
CN215678416U
CN215678416U CN202121878998.7U CN202121878998U CN215678416U CN 215678416 U CN215678416 U CN 215678416U CN 202121878998 U CN202121878998 U CN 202121878998U CN 215678416 U CN215678416 U CN 215678416U
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detection
cup
base
microplate reader
test strip
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宋健
王瑶
王鼎南
张宜明
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Zhejiang A&F University ZAFU
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Zhejiang A&F University ZAFU
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Abstract

The utility model discloses a multi-channel test strip determination plate compatible with a fluorescence microplate reader, which comprises a base and a cup strip assembly, wherein clamping grooves are formed in two sides of the upper surface of the base, a detection hole is formed in the upper surface of the base, the cup strip assembly comprises a determination cup, a connecting plate, a fixture block and partitions, the upper portion of the determination cup is fixedly inserted into the connecting plate, the lower portion of the determination cup is movably inserted into the detection hole, the upper end of the fixture block is fixedly connected with the connecting plate, the lower end of the fixture block is movably inserted into the clamping grooves, and the partitions are movably sleeved on two sides of the determination cup. In the utility model, the shape and the size of the base are consistent with those of a commercially available ninety-six-hole enzyme label plate, and the detection holes are matched with the commercially available ninety-six-hole enzyme label plate, so that the learning cost for operating the detection device is reduced, and a plurality of paper strips to be tested can be inserted to perform simultaneous detection of a plurality of samples.

Description

Multi-channel test strip determination plate compatible with fluorescent microplate reader
Technical Field
The utility model relates to the technical field of fluorescence determination, in particular to a multi-channel test strip determination plate compatible with a fluorescence microplate reader.
Background
The 96-hole fluorescent ELISA plate with the measuring plate of 8X12 used by the conventional ELISA reader is one of fluorescence measuring devices widely used at present, and is mainly characterized in that a light-tight black polystyrene tray, namely the bottom is black and light-tight, so as to be convenient for fluorescence detection, 96 small holes are arranged in the ELISA plate, each small hole can be used as a small test container, the size of the small test container is about 300 microliters, and the small test container can be used for placing homogeneous solution and can be widely applied to the fields of in-vitro diagnosis, rapid food safety analysis, environmental pollutant detection and the like.
At present, fluorescence test paper strip has obtained extensive application in food safety rapid analysis field, for realizing the sensitive reading of test paper strip fluorescence signal, the relevant producer has developed supporting fluorescence apparatus specially, and the key feature lies in with cutting ferrule encapsulation fluorescence test paper, and then inserts relevant fluorescence apparatus and detects, and this kind of single channel test mode is although can accurately read the fluorescence signal result, but detection efficiency is lower, has restricted the using value of ELIASA at fluorescence test paper analysis.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model can solve the problem of low single-channel detection mode efficiency of the existing test strip fluorescence detection device.
(II) technical scheme
In order to achieve the purpose, the utility model adopts the following technical scheme that the multi-channel test strip determination plate compatible with the fluorescence microplate reader comprises a base and a cup strip assembly;
clamping grooves are formed in two sides of the upper surface of the base, and detection holes are formed in the upper surface of the base;
the cup strip assembly comprises a measuring cup, a connecting plate, a clamping block and partitions, the upper portion of the measuring cup is fixedly connected to the connecting plate in an inserted mode, the lower portion of the measuring cup is movably connected to the detection hole in an inserted mode, the upper end of the clamping block is fixedly connected with the connecting plate, the lower end of the clamping block is movably connected to the clamping groove in an inserted mode, and the partitions are fixedly connected to the two sides of the bottom of the measuring cup.
As a preferred technical scheme of the present invention, the clamping grooves are V-shaped clamping grooves, eight pairs of the clamping grooves are provided, and each pair of the clamping grooves are symmetrically distributed along a center line of the base.
As a preferable technical solution of the present invention, the detecting holes are provided with eight rows, and each row is twelve detecting holes.
As a preferable technical scheme of the utility model, a hand-screwed bolt is installed on the side part of the base in a threaded manner, and one end of the hand-screwed bolt extends into the clamping groove.
As a preferred technical scheme of the present invention, the clamping block is a V-shaped clamping block matched with the clamping groove, a threaded hole is formed in a side wall of the clamping block, and the threaded hole is matched with the hand-screwed bolt.
In a preferred embodiment of the present invention, twelve measuring cups are provided, and twelve measuring cups are equidistantly distributed along the connecting plate.
As a preferable technical scheme of the utility model, the measuring cup and the partition are both made of black polystyrene material.
As a preferable technical solution of the present invention, the partition is abutted against an inner side wall of the inspection hole.
(III) advantageous effects
1. According to the multi-channel test strip assay plate compatible with the fluorescence microplate reader, the base is consistent with a commercially available ninety-six-hole elisa plate in shape and size, and the plurality of assay holes are formed in the base and matched with the commercially available ninety-six-hole elisa plate, so that on one hand, the learning cost for operating the assay device is reduced, the operation procedure is simplified, the labor cost for detection is reduced, the laboratory detection cost is remarkably reduced, a matched fluorescence detection instrument and matched operation software are not required to be redesigned or purchased, on the other hand, a plurality of test strips to be tested can be inserted, and the simultaneous detection of a plurality of samples is carried out, so that the problem that the single-channel detection mode efficiency of the traditional test strip fluorescence detector is low is avoided, the workload of operators is remarkably reduced, the detection efficiency of the samples is improved, and the detection cost is further reduced;
2. according to the multi-channel test strip determination plate compatible with the fluorescence microplate reader, the partitions are movably sleeved on the two sides of the determination cup, the test strip to be determined is inserted into the detection hole, and then the partitions and the determination cup are pressed on the test strip to be determined, so that the test strip to be determined can be conveniently fixed, and when a plurality of samples are detected simultaneously, fluorescent signals among different test strips can be effectively prevented from interfering with each other, and the stability of detection is ensured.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic top view of the base of the present invention;
FIG. 3 is a schematic top view of the cup bar assembly of the present invention;
FIG. 4 is a front schematic view of the cup bar assembly of the present invention;
FIG. 5 is a side view of the cartridge of the present invention;
FIG. 6 is a schematic top view of the partition of the present invention;
FIG. 7 is a first use state diagram of the present invention;
FIG. 8 is a second use state diagram illustration of the present invention;
fig. 9 is a third use state diagram of the present invention.
In the figure: 1. a base; 2. a cup strip assembly; 11. a card slot; 12. a detection hole; 13. screwing the bolt by hand; 21. a measuring cup; 22. a connecting plate; 23. a clamping block; 24. separating; 231. a threaded bore.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "longitudinal", "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Example 1
As shown in fig. 1-6, a multi-channel test strip assay plate compatible with a fluorescence microplate reader comprises a base 1 and a cup assembly 2, wherein the base 1 is used for placing the cup assembly 2, and the cup assembly 2 is used for being inserted into a test strip to be tested in cooperation with the base 1.
Referring to fig. 1 and 2, the shape and size of the base 1 are consistent with those of a commercially available ninety-six-hole elisa plate, so that the learning cost for operating the detection device is reduced, the operation procedure is simplified, the labor cost for detection is reduced, the detection cost in a laboratory is remarkably reduced, and a matched fluorescence detection instrument and matched operation software do not need to be redesigned or purchased;
the cup strip assembly comprises a base 1, and is characterized in that clamping grooves 11 are formed in two sides of the upper surface of the base 1 and used for being inserted into a cup strip assembly 2 in a matched mode, the clamping grooves 11 are arranged into V-shaped clamping grooves, eight pairs of clamping grooves 11 are formed in each clamping groove 11, and each pair of clamping grooves 11 are symmetrically distributed along the center line of the base 1;
the upper surface of the base 1 is provided with detection holes 12 for inserting test strips to be detected, the detection holes 12 are provided with eight rows, twelve detection holes 12 in each row are matched with a commercially available ninety-six-hole elisa plate.
Referring to fig. 1, 3, 4, 5 and 6, the cup strip assembly 2 includes a measuring cup 21, a connecting plate 22, a fixture block 23 and a partition 24;
the upper part of the measuring cup 21 is fixedly inserted on the connecting plate 22, the lower part of the measuring cup 21 is movably inserted in the detection hole 12 and is used for inserting the paper strip to be tested in cooperation with the detection hole 12, and the position of the measuring cup 21 also corresponds to the detection hole position of a ninety-six hole plate of a commercial microplate reader;
twelve measuring cups 21 are arranged, twelve measuring cups 21 are distributed at equal intervals along the connecting plate 22, so that the measuring cups 21 and the detection holes 12 can be matched for use;
the upper end of the fixture block 23 is fixedly connected with the connecting plate 22, and the lower end of the fixture block 23 is movably inserted into the clamping groove 11, so that the cup strip assembly 2 can be fixed on the base 1;
in order to further connect the clamping groove 11 and the clamping block 23 in a fastening manner, the clamping block 23 is a V-shaped clamping block matched with the clamping groove 11, a hand-screwed bolt 13 is installed on the side part of the base 1 in a threaded manner, one end of the hand-screwed bolt 13 extends into the clamping groove 11, a threaded hole 231 is formed in the side wall of the clamping block 23, and the threaded hole 231 is matched with the hand-screwed bolt 13;
the partitions 24 are fixedly connected to two sides of the bottom of the measuring cup 21, so that on one hand, the paper strips to be tested can be fixed by matching with the measuring cup 21, and on the other hand, when a plurality of samples are simultaneously detected, fluorescent signals among different test paper strips can be effectively prevented from interfering with each other, and the stability of detection is ensured;
the measuring cup 21 and the partition 24 are both made of black polystyrene material so as to facilitate fluorescence detection;
the partition 24 abuts against the inner side wall of the detection hole 12, so that the partition 24 and the detection hole 12 are fastened more tightly, and the test strip is compressed conveniently.
Referring to fig. 7, when using a cup assembly 2 and a test strip, firstly, after the test strip sucks a liquid to be tested, the test strip is placed on the base 1, so that the region to be tested of the test strip is opposite to the test hole 12 at the position B2, then, the fixture block 23 is inserted into the slot 11 at the position B, so as to ensure that the fixture block 23 is fastened with the slot 11, the test cup 21 is inserted into the test hole 12, so that the region to be tested of the test strip is tightly fixed on the upper part of the test cup 21, and meanwhile, the partition 24 is matched with the test cup 21 to fix the test strip to be tested, and finally, the whole device is placed into a fluorescence microplate reader for reading, and the reading of the microplate reader corresponding to the label B2 is recorded and further analyzed.
Example 2
As shown in fig. 1-6, a multi-channel test strip assay plate compatible with a fluorescence microplate reader comprises a base 1 and a cup assembly 2, wherein the base 1 is used for placing the cup assembly 2, and the cup assembly 2 is used for being inserted into a test strip to be tested in cooperation with the base 1.
Referring to fig. 1 and 2, the shape and size of the base 1 are consistent with those of a commercially available ninety-six-hole elisa plate, so that the learning cost for operating the detection device is reduced, the operation procedure is simplified, the labor cost for detection is reduced, the detection cost in a laboratory is remarkably reduced, and a matched fluorescence detection instrument and matched operation software do not need to be redesigned or purchased;
the cup strip assembly comprises a base 1, and is characterized in that clamping grooves 11 are formed in two sides of the upper surface of the base 1 and used for being inserted into a cup strip assembly 2 in a matched mode, the clamping grooves 11 are arranged into V-shaped clamping grooves, eight pairs of clamping grooves 11 are formed in each clamping groove 11, and each pair of clamping grooves 11 are symmetrically distributed along the center line of the base 1;
the upper surface of the base 1 is provided with detection holes 12 for inserting test strips to be detected, the detection holes 12 are provided with eight rows, twelve detection holes 12 in each row are matched with a commercially available ninety-six-hole elisa plate.
Referring to fig. 1, 3, 4, 5 and 6, the cup strip assembly 2 includes a measuring cup 21, a connecting plate 22, a fixture block 23 and a partition 24;
the upper part of the measuring cup 21 is fixedly inserted on the connecting plate 22, the lower part of the measuring cup 21 is movably inserted in the detection hole 12 and is used for inserting the paper strip to be tested in cooperation with the detection hole 12, and the position of the measuring cup 21 also corresponds to the detection hole position of a ninety-six hole plate of a commercial microplate reader;
twelve measuring cups 21 are arranged, twelve measuring cups 21 are distributed at equal intervals along the connecting plate 22, so that the measuring cups 21 and the detection holes 12 can be matched for use;
the upper end of the fixture block 23 is fixedly connected with the connecting plate 22, and the lower end of the fixture block 23 is movably inserted into the clamping groove 11, so that the cup strip assembly 2 can be fixed on the base 1;
in order to further connect the clamping groove 11 and the clamping block 23 in a fastening manner, the clamping block 23 is a V-shaped clamping block matched with the clamping groove 11, a hand-screwed bolt 13 is installed on the side part of the base 1 in a threaded manner, one end of the hand-screwed bolt 13 extends into the clamping groove 11, a threaded hole 231 is formed in the side wall of the clamping block 23, and the threaded hole 231 is matched with the hand-screwed bolt 13;
the partitions 24 are fixedly connected to two sides of the bottom of the measuring cup 21, so that on one hand, the paper strips to be tested can be fixed by matching with the measuring cup 21, and on the other hand, when a plurality of samples are simultaneously detected, fluorescent signals among different test paper strips can be effectively prevented from interfering with each other, and the stability of detection is ensured;
the measuring cup 21 and the partition 24 are both made of black polystyrene material so as to facilitate fluorescence detection;
the partition 24 abuts against the inner side wall of the detection hole 12, so that the partition 24 and the detection hole 12 are fastened more tightly, and the test strip is compressed conveniently.
Referring to fig. 8, when using one cup assembly 2 and a plurality of test strips for detecting fluorescence color development of different liquids under the action of the same fluorescence (monochromatic fluorescence), firstly, after the test strips absorb the liquid to be detected, the test strips are placed on a base 1, the areas to be detected of the test strips are respectively opposite to the detection holes 12 at the positions of C3, C5, C7 and C9, then, the fixture blocks 23 are inserted into the clamp grooves 11 at the position of C, the fixture blocks 23 are ensured to be fastened with the clamp grooves 11, the measuring cups 21 are inserted into the detection holes 12, the areas to be detected of the test strips are tightly fixed on the upper portions of the measuring cups 21, meanwhile, the partitions 24 are matched with the measuring cups 21 to fix the test strips to be detected, and finally, the whole device is placed into a fluorescence microplate reader for reading, and the readings of microplate readers corresponding to the marks C3, C5, C7 and C9 are recorded and further analyzed.
Example 3
As shown in fig. 1-6, a multi-channel test strip assay plate compatible with a fluorescence microplate reader comprises a base 1 and a cup assembly 2, wherein the base 1 is used for placing the cup assembly 2, and the cup assembly 2 is used for being inserted into a test strip to be tested in cooperation with the base 1.
Referring to fig. 1 and 2, the shape and size of the base 1 are consistent with those of a commercially available ninety-six-hole elisa plate, so that the learning cost for operating the detection device is reduced, the operation procedure is simplified, the labor cost for detection is reduced, the detection cost in a laboratory is remarkably reduced, and a matched fluorescence detection instrument and matched operation software do not need to be redesigned or purchased;
the cup strip assembly comprises a base 1, and is characterized in that clamping grooves 11 are formed in two sides of the upper surface of the base 1 and used for being inserted into a cup strip assembly 2 in a matched mode, the clamping grooves 11 are arranged into V-shaped clamping grooves, eight pairs of clamping grooves 11 are formed in each clamping groove 11, and each pair of clamping grooves 11 are symmetrically distributed along the center line of the base 1;
the upper surface of the base 1 is provided with detection holes 12 for inserting test strips to be detected, the detection holes 12 are provided with eight rows, twelve detection holes 12 in each row are matched with a commercially available ninety-six-hole elisa plate.
Referring to fig. 1, 3, 4, 5 and 6, the cup strip assembly 2 includes a measuring cup 21, a connecting plate 22, a fixture block 23 and a partition 24;
the upper part of the measuring cup 21 is fixedly inserted on the connecting plate 22, the lower part of the measuring cup 21 is movably inserted in the detection hole 12 and is used for inserting the paper strip to be tested in cooperation with the detection hole 12, and the position of the measuring cup 21 also corresponds to the detection hole position of a ninety-six hole plate of a commercial microplate reader;
twelve measuring cups 21 are arranged, twelve measuring cups 21 are distributed at equal intervals along the connecting plate 22, so that the measuring cups 21 and the detection holes 12 can be matched for use;
the upper end of the fixture block 23 is fixedly connected with the connecting plate 22, and the lower end of the fixture block 23 is movably inserted into the clamping groove 11, so that the cup strip assembly 2 can be fixed on the base 1;
in order to further connect the clamping groove 11 and the clamping block 23 in a fastening manner, the clamping block 23 is a V-shaped clamping block matched with the clamping groove 11, a hand-screwed bolt 13 is installed on the side part of the base 1 in a threaded manner, one end of the hand-screwed bolt 13 extends into the clamping groove 11, a threaded hole 231 is formed in the side wall of the clamping block 23, and the threaded hole 231 is matched with the hand-screwed bolt 13;
the partitions 24 are fixedly connected to two sides of the bottom of the measuring cup 21, so that on one hand, the paper strips to be tested can be fixed by matching with the measuring cup 21, and on the other hand, when a plurality of samples are simultaneously detected, fluorescent signals among different test paper strips can be effectively prevented from interfering with each other, and the stability of detection is ensured;
the measuring cup 21 and the partition 24 are both made of black polystyrene material so as to facilitate fluorescence detection;
the partition 24 abuts against the inner side wall of the detection hole 12, so that the partition 24 and the detection hole 12 are fastened more tightly, and the test strip is compressed conveniently.
Referring to fig. 9, when two cup assemblies 2 and one test strip are used, the test strip is placed on a base 1 after absorbing liquid to be tested, so that test areas of the test strip are respectively opposite to detection holes 12 at positions B4 and D4, then clamping blocks 23 of the two cup assemblies 2 are respectively inserted into clamping grooves 11 at positions B, D, the clamping blocks 23 are fastened with the clamping grooves 11, a measuring cup 21 is inserted into the detection holes 12, so that the test areas to be tested of the test strip are tightly fixed on the upper portion of the measuring cup 21, meanwhile, a partition 24 is matched with the measuring cup 21 to fix the test strip to be tested, and finally, the whole device is placed into a fluorescence microplate reader for reading, and readings of microplate readers corresponding to the labels B4 and D4 are recorded and further analyzed.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. The utility model provides a compatible fluorescence ELIASA's multichannel test paper strip survey board which characterized in that includes:
the detection device comprises a base (1), wherein clamping grooves (11) are formed in two sides of the upper surface of the base (1), and detection holes (12) are formed in the upper surface of the base (1);
the cup strip assembly (2) comprises a measuring cup (21), a connecting plate (22), a clamping block (23) and a partition (24), the upper portion of the measuring cup (21) is fixedly connected to the connecting plate (22) in an inserted mode, the lower portion of the measuring cup (21) is movably connected to the detection hole (12) in an inserted mode, the upper end of the clamping block (23) is fixedly connected with the connecting plate (22), the lower end of the clamping block (23) is movably connected to the clamping groove (11) in an inserted mode, and the partition (24) is fixedly connected to two sides of the bottom of the measuring cup (21).
2. The multi-channel test strip determination plate compatible with the fluorescence microplate reader as claimed in claim 1, wherein the slots (11) are V-shaped slots, eight pairs of slots (11) are provided, and each pair of slots (11) are symmetrically distributed along the central line of the base (1).
3. The microplate reader-compatible multi-channel test strip assay plate of claim 1, wherein the detection wells (12) are provided in eight rows of twelve detection wells (12) per row.
4. The multi-channel test strip determination plate compatible with the fluorescence microplate reader as claimed in claim 1, wherein a hand-screwed bolt (13) is installed on the side portion of the base (1) in a threaded manner, and one end of the hand-screwed bolt (13) extends into the clamping groove (11).
5. The multi-channel test strip determination plate compatible with the fluorescence microplate reader as defined in claim 4, wherein the fixture block (23) is a V-shaped fixture block matched with the slot (11), a threaded hole (231) is formed in a side wall of the fixture block (23), and the threaded hole (231) is matched with the hand-screwed bolt (13).
6. The microplate reader-compatible multi-channel strip assay plate of claim 1, wherein twelve of said assay cups (21) are provided and equally spaced along said web (22).
7. The microplate reader-compatible multi-channel test strip assay plate of claim 1, wherein the assay cup (21) and the partition (24) are both provided as assay cups (21) of black polystyrene material.
8. The microplate reader-compatible multi-channel strip assay plate of claim 1, wherein the partition (24) abuts an interior sidewall of the detection well (12).
CN202121878998.7U 2021-08-12 2021-08-12 Multi-channel test strip determination plate compatible with fluorescent microplate reader Active CN215678416U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121878998.7U CN215678416U (en) 2021-08-12 2021-08-12 Multi-channel test strip determination plate compatible with fluorescent microplate reader

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121878998.7U CN215678416U (en) 2021-08-12 2021-08-12 Multi-channel test strip determination plate compatible with fluorescent microplate reader

Publications (1)

Publication Number Publication Date
CN215678416U true CN215678416U (en) 2022-01-28

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