CN218512457U - Colloidal gold detection card - Google Patents

Colloidal gold detection card Download PDF

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Publication number
CN218512457U
CN218512457U CN202222382299.4U CN202222382299U CN218512457U CN 218512457 U CN218512457 U CN 218512457U CN 202222382299 U CN202222382299 U CN 202222382299U CN 218512457 U CN218512457 U CN 218512457U
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China
Prior art keywords
colloidal gold
sample
detection
pad
cover plate
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CN202222382299.4U
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智军海
万峰
周嘉明
孟超
封冰
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Tianjin Hua Test Testing And Certification Co ltd
Centre Testing International Group Co ltd
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Tianjin Hua Test Testing And Certification Co ltd
Centre Testing International Group Co ltd
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Priority to CN202222382299.4U priority Critical patent/CN218512457U/en
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Abstract

The utility model relates to a food safety inspection technical field, a colloidal gold detects card is provided, comprising a base plate and a cover plate, through setting up a plurality of indexes of many colloidal gold detection test paper strip detectable same samples, through the total application of sample hole on the cover plate, can detect many colloidal gold detection test paper strips while application of sample between cover plate and base plate, judge the detection information that the window can read the result display area of every colloidal gold detection test paper strip and show through the result on the cover plate, thereby obtain a plurality of detection index testing results of same sample, the purpose of a plurality of detection items of once application of sample while detection of single sample has been realized, be favorable to saving check-out time, improve detection efficiency.

Description

Colloidal gold detection card
Technical Field
The application belongs to the technical field of food safety detection, and more particularly relates to a colloidal gold detection card.
Background
The existing food safety detection methods mainly comprise gas chromatography, liquid chromatography and chromatography-mass spectrometry, and although the methods have the advantages of high sensitivity, accurate result, good repeatability, few false positives and the like, the methods have the defects of complex sample pretreatment process, high professional requirement of detection instruments, expensive instruments, low analysis speed, high detection cost, incapability of performing field detection and the like. Compared with gas chromatography, liquid chromatography and chromatography-mass spectrometry, the colloidal gold immunochromatography method has the advantages of high sensitivity, strong specificity, simple sample pretreatment and short detection time, and is suitable for field monitoring and screening of a large number of samples.
The existing colloidal gold detection card is generally a regular symmetrical or irregular trapezoid detection card made of engineering plastics (nylon mixed glass fiber), and the detection card contains one detection test paper, namely only one item can be detected at one time; or a plurality of parallel test paper is contained, but in the detection process, the detection liquid needs to be repeatedly absorbed and dripped into the total sample adding hole for many times, the efficiency of the detection process is low, the detection process is complicated, and the using effect is not good.
SUMMERY OF THE UTILITY MODEL
An object of the embodiment of the application is to provide a colloidal gold detection card to solve the technical problems that the detection card capable of detecting multiple indexes of the same sample in the prior art is complex to operate and low in efficiency in the actual detection operation process.
In order to achieve the purpose, the technical scheme adopted by the application is as follows: provided is a colloidal gold detection card including: the device comprises a bottom plate, a cover plate and a plurality of colloidal gold detection test strips, wherein the bottom plate is connected with the cover plate, the plurality of colloidal gold detection test strips are clamped between the bottom plate and the cover plate, and each colloidal gold detection test strip is used for detecting one corresponding index of a sample;
the cover plate is provided with a total sample adding hole, a result interpretation window is arranged corresponding to the position of the colloidal gold detection test strip, the sample adding end of each colloidal gold detection test strip is communicated with the total sample adding hole, and the result display area of each colloidal gold detection test strip is exposed to the result interpretation window.
Optionally, an identification code is sprayed on the cover plate corresponding to the result display area of each colloidal gold test strip.
Optionally, the result interpretation window includes a plurality of sub-windows arranged at intervals, and the sub-windows, the colloidal gold test strips, and the identification codes are arranged in a one-to-one correspondence manner.
Optionally, many colloidal gold test paper strips are fixed in on the bottom plate, corresponding to sample adding hole position has seted up the sample holding tank on the bottom plate, every colloidal gold test paper strip the application of sample end all stretch into to in the sample holding tank.
Optionally, total adding the appearance hole with the sample holding tank all is circular, just total adding the appearance hole and being located the central point of apron puts, the sample holding tank is located the central point of bottom plate puts, many the colloidal gold detects the test paper strip with the sample holding tank is radial evenly distributed as the center.
Optionally, the number of the colloidal gold test strips is less than or equal to three, the cover plate and the bottom plate are in a fan shape matched with each other, and the total sample adding hole and the sample accommodating groove are both located at the circle center of the fan shape.
Optionally, the number of the colloidal gold test strips is more than three, and the cover plate and the bottom plate are in a mutually matched circular shape.
Optionally, each colloidal gold detection test strip includes a substrate, a sample pad, a gold-labeled pad, a nitrocellulose membrane, and a water absorption pad, the nitrocellulose membrane is disposed by being attached to the upper surface of the substrate, the upper surface of the nitrocellulose membrane is provided with the result display area, one end of the water absorption pad is disposed at a first end of the nitrocellulose membrane, the first end of the gold-labeled pad is disposed at a second end of the nitrocellulose membrane, the first end of the sample pad is disposed at the second end of the gold-labeled pad, and the second end of the sample pad is communicated with the total sample adding hole;
and a detection substance corresponding to the detection index of each colloidal gold detection test strip is arranged on the gold label pad of each colloidal gold detection test strip.
Optionally, a detection line close to the sample pad and a quality control line close to the absorbent pad are disposed in the result display region of each colloidal gold detection test strip, and the detection line and the quality control line can be exposed through the result interpretation window.
Optionally, the substrate is a polyvinyl chloride member, and the nitrocellulose membrane is adhered to the upper surface of the substrate.
The application provides a colloidal gold detects card's beneficial effect lies in: compared with the prior art, the colloidal gold test card of this application can detect a plurality of indexes of the same sample through setting up many colloidal gold test paper strips, through a total application of sample hole on the apron, can be to setting up many colloidal gold test paper strips between apron and bottom plate application of sample simultaneously, judge the detection information that the result display area that the window can read every colloidal gold test paper strip shows through the result on the apron, thereby obtain a plurality of test index testing results of same sample, the purpose of having realized that the application of sample once of single sample detects a plurality of test items simultaneously, be favorable to saving check-out time, improve detection efficiency.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required to be used in the embodiments or the prior art description will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings may be obtained according to these drawings without inventive labor.
Fig. 1 is a schematic structural diagram of a colloidal gold detection card cover plate (circular) according to an embodiment of the present disclosure;
fig. 2 is a schematic structural diagram of a bottom plate (circular) of a colloidal gold test card provided in an embodiment of the present application;
fig. 3 is a schematic structural diagram of a colloidal gold detection card cover plate (sector) according to an embodiment of the present disclosure;
fig. 4 is a schematic structural diagram of a bottom plate (sector) of a colloidal gold test card provided in an embodiment of the present application;
fig. 5 is a schematic structural diagram of a colloidal gold test strip provided in the embodiment of the present application.
Wherein, in the figures, the respective reference numerals:
1. a base plate; 2. a cover plate; 3. a total sampling hole; 4. a result interpretation window; 5. an identification code; 6. colloidal gold test paper; 6a, a substrate; 6b, sample pad; 6c, a gold mark pad; 6d, a nitrocellulose membrane; 6e, a water absorption pad; 6f, detecting lines; 6g, quality control line.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of and not restrictive on the broad application.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
The colloidal gold test card provided by the embodiment of the application is explained by combining the accompanying drawings as follows:
referring to fig. 1 to 4, the colloidal gold test card disclosed in the present application includes a bottom plate 1, a cover plate 2 and a plurality of colloidal gold test strips 6, wherein the bottom plate 1 is connected to the cover plate 2, and in different embodiments, the bottom plate 1 and the cover plate 2 may be selectively connected in different manners, such as detachable connection, fixed connection, snap connection, threaded connection, and the like. The specific shapes of the bottom plate 1 and the cover plate 2 are not limited, such as round, square, polygon or other special-shaped structures.
The plurality of colloidal gold test strips 6 are clamped between the bottom plate 1 and the cover plate 2, and each colloidal gold test strip 6 is used for detecting a corresponding index of a sample. Many colloidal gold test paper strips 6 set up between bottom plate 1 and apron 2, and colloidal gold test paper strip 6 can be fixed in on bottom plate 1, also can be fixed in on the apron 2, plays the effect of fixed stay and isolation protection to colloidal gold test paper strip 6 through bottom plate 1 and apron 2.
Wherein, the cover plate 2 is provided with a total sample adding hole 3, and a result interpretation window 4 is provided corresponding to the position of the colloidal gold test strip 6, the sample adding end of each colloidal gold test strip 6 is communicated with the total sample adding hole 3, namely, each colloidal gold test strip 6 can be dropwise added with a sample through the total sample adding hole 3, so that one-time sample adding operation can meet the detection requirements of a plurality of colloidal gold test strips 6. The result display area of each colloidal gold test strip 6 is exposed on the result interpretation window 4. An operator or a detection instrument can read the detection result displayed in the result display area of each colloidal gold detection test strip 6 through the result interpretation window 4. The specific shape of the result interpretation window 4 can be set as required, for example, a corresponding separate result interpretation window 4 is set for each colloidal gold detection test strip 6, the specific shape of a single result interpretation window 4 is not limited, and corresponding design can be performed according to actual requirements, for example, the design is circular, square, polygonal or trapezoidal; in other embodiments, a total result interpretation window 4 may be provided as needed, so that the result display area of each colloidal gold test strip 6 can be displayed through the result interpretation window 4, for example, the result interpretation window 4 may be provided in a continuous ring shape.
Compared with the prior art, many colloidal gold test paper strip 6 in this application all communicates with total application of sample hole 3, a corresponding index of every colloidal gold test paper strip 6 test sample, operating personnel adds the application of sample through total application of sample hole 3 once, can realize detecting the multinomial index of same sample, operating personnel or instrument judge window 4 through the result and observe or acquire the testing result on the colloidal gold test paper strip 6, the number of times of application of sample when having reduced the detection, shorten check-out time, detection efficiency has been improved.
In an embodiment of the present application, an identification code 5 is sprayed on the cover plate 2 at a position corresponding to the result display area of each colloidal gold test strip 6. The identification code 5 is scanned by corresponding scanning equipment, so that the detailed information of the detection item corresponding to each colloidal gold detection test strip 6 can be obtained, and the method is convenient and rapid. The identification code 5 can be selected according to the requirement, such as two-dimensional code, bar code, etc.
In another embodiment of the present application, preferably, the result interpretation window 4 includes a plurality of sub-windows arranged at intervals, and the sub-windows, the colloidal gold test strips 6 and the identification codes 5 are arranged in a one-to-one correspondence. The instrument can identify and record the detection item information by scanning the identification code 5 corresponding to the sub-window. Specifically, in this embodiment, the result display area on each colloidal gold test strip 6 can be exposed through the corresponding sub-window. The shape of the sub-window can be designed according to actual needs, for example, the sub-window can be set to be a trapezoid.
In another embodiment of the present application, a plurality of colloidal gold test strips 6 are fixed on the bottom plate 1, a sample holding groove is formed on the bottom plate 1 corresponding to the position of the total sample adding hole 3, and the sample adding end of each colloidal gold test strip 6 extends into the sample holding groove. The sample holding tank is used for transferring and temporarily storing the sample solution, and the sample contacts a plurality of colloidal gold test strips 6 after entering the sample holding tank and is diffused to the colloidal gold test strips 6.
In another embodiment of this application, total application of sample hole 3 and sample holding tank all are circularly, and total application of sample hole 3 is located the central point of apron 2 and puts, and the sample holding tank is located the central point of bottom plate 1, and many colloidal gold test paper strips 6 use the sample holding tank to be radial evenly distributed as the center.
In order to avoid the sample to differ in the diffusion volume of colloidal gold test paper strip 6 of difference, produce and detect the deviation, the sample holding tank setting in this application is in the centre of a circle department of bottom plate 1, and colloidal gold test paper strip 6 is radial equidistance and sets up in the periphery of sample holding tank, and the distance between the adjacent colloidal gold test paper strip 6 equals promptly. After the sample dripped into in the sample holding tank, simultaneously with the contact of a plurality of colloidal gold test paper strips 6, the sample was the same at the diffusion volume on a plurality of colloidal gold test paper strips 6, reduces the difference, avoids producing the detection deviation.
In another embodiment of the present application, please refer to fig. 3 and 4, the number of the colloidal gold test strips 6 is less than or equal to three, the cover plate 2 and the bottom plate 1 are in the shape of sectors matched with each other, and the total sample adding hole 3 and the sample accommodating groove are both located at the center of the sector. Illustratively, when the number of the colloidal gold test strips 6 is one, two or three, the shapes of the cover plate 2 and the bottom plate 1 are fan-shaped and matched with each other.
In another embodiment of the present application, referring to fig. 1 and 2, the number of the colloidal gold test strips 6 is more than three, and the cover plate 2 and the base plate 1 are circular and matched with each other. Illustratively, when the number of the colloidal gold test strips 6 is four, five, six or more, the cover plate 2 and the base plate 1 are circular and match each other. The circular and fan-shaped design can ensure that the sample chromatographic distance and the chromatographic quantity between different colloidal gold test strips 6 are consistent, and reduce the difference between different colloidal gold test strips 6, thereby being beneficial to ensuring the accuracy of the test result.
In another embodiment of the present application, referring to fig. 5, each colloidal gold test strip 6 includes a substrate 6a, a sample pad 6b, a gold-labeled pad 6c, a nitrocellulose membrane 6d, and a water absorption pad 6e, the nitrocellulose membrane 6d is disposed adjacent to the upper surface of the substrate 6a, the upper surface of the nitrocellulose membrane 6d is provided with a result display area, one end of the water absorption pad 6e is overlapped on the first end of the nitrocellulose membrane 6d, the first end of the gold-labeled pad 6c is overlapped on the second end of the nitrocellulose membrane 6d, the first end of the sample pad 6b is overlapped on the second end of the gold-labeled pad 6c, and the second end of the sample pad 6b is communicated with the total sample adding hole 3.
Specifically, in the present embodiment, the side close to the sample pad 6b is defined as the second end, and the side close to the absorbent pad 6e is defined as the first end. Under the adsorption force of the absorbent pad 6e, the sample solution flows from the second end to the first end, i.e. the sample solution enters from the total sample adding hole 3, first contacts the second end of the sample pad 6b, and then contacts the gold label pad 6c and the nitrocellulose membrane 6d in sequence.
The gold pad 6c of each colloidal gold test strip 6 is provided with a detection substance corresponding to the detection index of the colloidal gold test strip 6. By arranging different detection substances on the gold label pad 6c, the sample solution can react with the substances on the different gold label pads 6c to generate different gold label compounds, so that the detection result information of different indexes, which are required to be detected by the sample solution, can be indicated.
In another embodiment of the present application, a detection line 6f near the sample pad 6b and a quality control line 6g near the absorbent pad 6e are disposed in the result display area of each colloidal gold test strip 6, and both the detection line 6f and the quality control line 6g can be exposed through the result interpretation window 4. The corresponding detection result can be clearly shown through the arranged detection line 6f and the quality control line 6g, so that an operator or a detection instrument can read the detection result easily through the result interpretation window 4.
In another embodiment of the present application, the substrate 6a is a polyvinyl chloride member, and the nitrocellulose membrane 6d is adhered to the upper surface of the substrate 6 a. The substrate 6a is made of polyvinyl chloride, so that certain supporting strength can be provided on the premise of ensuring that the sample solution is not absorbed, and the supporting and fixing of each functional pad on the colloidal gold detection test strip 6 are facilitated. The nitrocellulose membrane 6d is connected with the substrate 6a in a pasting mode, the connection relationship is simple and firm, and the cellulose nitrate membrane 6d cannot shift or fall off in the moving or transporting process of the colloidal gold detection card.
The detection principle of the colloidal gold detection card of the embodiment of the application is as follows:
during detection, the detection card is horizontally placed, a sample solution to be detected is dripped into the total sample adding hole 3, the sample solution is subjected to chromatography along a plurality of colloidal gold detection test strips 6 simultaneously, on each colloidal gold detection test strip 6, the sample pad 6b is firstly contacted with the sample solution, and the sample solution permeates into the gold label pad 6c of the colloidal gold detection test strip 6 through the sample pad 6 b; the gold-labeled pad 6c on each colloidal gold test strip 6 contacts with the sample solution to react to generate different gold-labeled compounds, meanwhile, under the action of the water absorption pad 6e, the gold-labeled compounds move forward continuously, namely move towards the direction close to the water absorption pad 6e and react with the test line 6f and the quality control line 6g on the nitrocellulose membrane 6d, and finally, the redundant sample solution and the gold-labeled compounds are absorbed by the water absorption pad 6e at the tail end of the colloidal gold test strip 6 to prevent the sample solution from leaking. After the reaction is finished, an operator or a detection instrument can judge the detection result through the color development conditions of the detection line 6f and the quality control line 6 g. Therefore, the purpose that multiple indexes can be detected simultaneously by one-time sample adding of the same sample solution is achieved, the detection time is saved, and the detection efficiency is improved.
The present invention is not intended to be limited to the particular embodiments shown and described, but is to be accorded the widest scope consistent with the principles and novel features herein disclosed.

Claims (10)

1. A colloidal gold test card is characterized by comprising a bottom plate, a cover plate and a plurality of colloidal gold test strips, wherein the bottom plate is connected with the cover plate, the plurality of colloidal gold test strips are clamped between the bottom plate and the cover plate, and each colloidal gold test strip is used for testing one corresponding index of a sample;
the cover plate is provided with a total sample adding hole, a result interpretation window is arranged corresponding to the position of the colloidal gold detection test strip, the sample adding end of each colloidal gold detection test strip is communicated with the total sample adding hole, and the result display area of each colloidal gold detection test strip is exposed to the result interpretation window.
2. The colloidal gold test card of claim 1, wherein an identification code is sprayed on the cover plate at a position corresponding to the result display area of each colloidal gold test strip.
3. The gold assay card of claim 2 wherein the result interpretation window comprises a plurality of spaced sub-windows, the gold assay strips and the identification codes being arranged in a one-to-one correspondence.
4. The gold test card of claim 1, wherein a plurality of gold test strips are fixed on the bottom plate, a sample receiving slot is formed in the bottom plate at a position corresponding to the total sample adding hole, and the sample adding end of each gold test strip extends into the sample receiving slot.
5. The gold reagent card of claim 4, wherein the total sample hole and the sample holding groove are circular, the total sample hole is located at the center of the cover plate, the sample holding groove is located at the center of the bottom plate, and the gold reagent strips are radially and uniformly distributed around the sample holding groove.
6. The colloidal gold test card of claim 5, wherein the number of the colloidal gold test strips is less than or equal to three, the cover plate and the bottom plate are in the shape of a fan which is matched with each other, and the total sample adding hole and the sample accommodating groove are both located at the center of the fan.
7. The colloidal gold test card of claim 5, wherein the number of the colloidal gold test strips is more than three, and the cover plate and the bottom plate are in the shape of mutually matched circles.
8. The colloidal gold test card of any one of claims 1 to 7, wherein each of the colloidal gold test strips comprises a substrate, a sample pad, a gold-labeled pad, a nitrocellulose membrane, and a water-absorbing pad, wherein the nitrocellulose membrane is disposed against an upper surface of the substrate, the upper surface of the nitrocellulose membrane is provided with the result display region, one end of the water-absorbing pad is disposed on a first end of the nitrocellulose membrane, a first end of the gold-labeled pad is disposed on a second end of the nitrocellulose membrane, a first end of the sample pad is disposed on a second end of the gold-labeled pad, and a second end of the sample pad is in communication with the total sample-adding hole;
and a detection substance corresponding to the detection index of each colloidal gold detection test strip is arranged on the gold label pad of each colloidal gold detection test strip.
9. The colloidal gold test card of claim 8, wherein each of the colloidal gold test strips has a test line adjacent to the sample pad and a quality control line adjacent to the absorbent pad in the result display region, and wherein the test line and the quality control line are exposed through the result interpretation window.
10. The colloidal gold test card according to claim 8, wherein the substrate is a polyvinyl chloride member, and the nitrocellulose membrane is adhered to an upper surface of the substrate.
CN202222382299.4U 2022-09-08 2022-09-08 Colloidal gold detection card Active CN218512457U (en)

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Application Number Priority Date Filing Date Title
CN202222382299.4U CN218512457U (en) 2022-09-08 2022-09-08 Colloidal gold detection card

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Application Number Priority Date Filing Date Title
CN202222382299.4U CN218512457U (en) 2022-09-08 2022-09-08 Colloidal gold detection card

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116359498A (en) * 2023-02-23 2023-06-30 兰州兽研生物科技有限公司 Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116359498A (en) * 2023-02-23 2023-06-30 兰州兽研生物科技有限公司 Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies

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