CN215627981U - Liquid supplementing and taking device for cell culture - Google Patents
Liquid supplementing and taking device for cell culture Download PDFInfo
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- CN215627981U CN215627981U CN202122117852.7U CN202122117852U CN215627981U CN 215627981 U CN215627981 U CN 215627981U CN 202122117852 U CN202122117852 U CN 202122117852U CN 215627981 U CN215627981 U CN 215627981U
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- thick bamboo
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Abstract
The utility model relates to the technical field of cell culture, in particular to a liquid supplementing and taking device for cell culture, which comprises a liquid storage cylinder, wherein the lower end of the liquid storage cylinder is welded with a liquid taking port, the joint of the liquid storage cylinder and the liquid taking port is welded with a lower connecting piece, the upper end of the liquid storage cylinder is welded with a supporting plate, two symmetrically arranged sleeves are welded on two sides of the upper surface of the lower connecting piece, the sleeves penetrate through the supporting plate and are welded in the supporting plate, a movable plug is connected in the liquid storage cylinder in a sliding manner, each sleeve is connected with a measuring mechanism in a sliding manner, when the liquid storage cylinder is used for absorbing cell culture liquid supplementing, the liquid capacity in the liquid storage cylinder is read through a scale area of a scale column of the measuring mechanism, then the positioning mechanism is pulled up according to the requirement, a positioning block is inserted into a limiting groove, the maximum falling height of the movable plug is limited, and the injection amount is controlled, the situation that the traditional liquid supplementing injection mode cannot accurately control the quantity is improved.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a liquid supplementing and taking device for cell culture.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. The cell culture technology is an important and common technology in cell biology research methods, a large number of cells can be obtained through cell culture, signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells and the like can also be researched through the cell culture technology, when certain nutrition is lacked in the cell culture process, cell culture solution needs to be added to a culture dish again in order to ensure normal operation of the culture, but the cell culture solution can only be quantitatively added, and the cell growth can be influenced by a large amount of the cell culture solution. In view of this, we propose a fluid infusion and extraction device for cell culture.
SUMMERY OF THE UTILITY MODEL
The utility model provides a liquid supplementing and taking device for cell culture, which aims to solve the problems in the background technology.
In order to achieve the purpose, the utility model provides the following technical scheme:
the utility model provides a cell culture is with fluid infusion liquid taking device, is including depositing a liquid section of thick bamboo, deposit liquid section of thick bamboo lower extreme welding and get the liquid mouth, deposit a liquid section of thick bamboo with it has lower connecting piece to get the welding of liquid mouth junction, it has the backup pad to deposit liquid section of thick bamboo upper end welding, the welding of both sides has the sleeve that is the symmetry and places on the connecting piece upper surface down, the sleeve passes the backup pad weld in the backup pad, deposit liquid section of thick bamboo sliding connection has the activity to fill in, every equal sliding connection has measuring mechanism in the sleeve, the activity fill in with the measuring mechanism upper end is through last connecting piece fixed connection, the welding has the spout on the backup pad side surface, sliding connection has positioning mechanism in the spout.
As a preferred technical scheme, the measuring mechanism comprises a scale column which is slidably connected in the sleeve, a scale area which is arranged on the left surface of the scale column and a limit area which is arranged on the right surface of the scale column.
Preferably, the positioning mechanism includes a connecting member slidably connected to an outer surface of the liquid storage cylinder, supporting columns welded to two ends of an upper surface of the connecting member, and a positioning block rotatably connected to top ends of the supporting columns, and the supporting columns are slidably connected to the inside of the sliding grooves.
As the preferred technical scheme, the top welding has the fender ring in depositing a liquid section of thick bamboo, the welding of activity stopper bottom has the rubber buffer.
As a preferred technical scheme, the whole supporting plate is of a cross structure, and the upper connecting piece and the lower connecting piece are of a straight-line structure.
As a preferred technical scheme, the limiting area comprises a plurality of limiting grooves formed in the right side surface of the scale column.
As a preferable technical scheme, the positioning block can rotate 360 degrees at the top end of the support column.
Compared with the prior art, the utility model has the beneficial effects that: after using and depositing a liquid section of thick bamboo and absorbing cell culture fluid replacement, read through the scale district of measuring mechanism scale post and deposit liquid capacity in the liquid section of thick bamboo, later according to the demand, draw up with that positioning mechanism, peg graft the locating piece to the spacing inslot, restrict the activity stopper maximum height of whereabouts, realize controlling the injection volume, improved the unable accurate controlled volume's of traditional fluid replacement injection mode situation.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the liquid storage cylinder according to the present invention;
FIG. 3 is a schematic view of the measuring mechanism according to the present invention;
FIG. 4 is a schematic structural view of a limiting groove according to the present invention;
fig. 5 is a schematic structural view of the positioning mechanism of the present invention.
In the figure:
a liquid storage cylinder 1; a sleeve 2; a support plate 3;
a measuring mechanism 4; a scale post 41; a scale region 42; a stopper region 43; a retaining groove 431;
a movable plug 5; an upper connecting piece 6;
a positioning mechanism 7; a connecting member 71; a support post 72; a positioning block 73;
a lower connecting member 8; a liquid taking port 9; a retainer ring 10; a rubber stopper 11; a chute 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Referring to fig. 1-5, the present embodiment provides a technical solution:
the utility model provides a cell culture is with fluid infusion liquid taking device, including depositing a liquid section of thick bamboo 1, deposit 1 lower extreme welding of a liquid section of thick bamboo and get liquid mouth 9, deposit a liquid section of thick bamboo 1 and get liquid mouth 9 junction welding and have connecting piece 8 down, deposit 1 upper end welding of a liquid section of thick bamboo and have backup pad 3, the both sides welding has sleeve 2 that is the symmetry and places on the 8 upper surface of lower connecting piece, sleeve 2 passes backup pad 3 and welds in backup pad 3, it has movable stopper 5 to deposit 1 sliding connection in the liquid section of thick bamboo, equal sliding connection has measuring mechanism 4 in every sleeve 2, 6 fixed connection are passed through on movable stopper 5 and the measuring mechanism 4, the welding has spout 12 on the 3 side surfaces of backup pad, sliding connection has positioning mechanism 7 in spout 12.
It should be added that the measuring mechanism 4 includes a scale column 41 slidably connected in the sleeve 2, a scale area 42 provided on the left side surface of the scale column 41, and a limit area 43 provided on the right side surface of the scale column 41, and the scale area 42 is used for marking the measurement of the volume of the liquid in the liquid storage cylinder 1.
Preferably, the positioning mechanism 7 includes a connecting member 71 slidably connected to the outer surface of the liquid storage cylinder 1, supporting columns 72 welded to two ends of the upper surface of the connecting member 71, and a positioning block 73 rotatably connected to the top ends of the supporting columns 72, wherein the supporting columns 72 are slidably connected to the inside of the sliding grooves 12, the connecting member 71 has an arc structure attached to the outer surface of the liquid storage cylinder 1, and the positioning mechanism 7 is adapted to slide around the liquid storage cylinder 1 as a whole.
Preferably, a baffle ring 10 is welded at the top end in the liquid storage cylinder 1, a rubber plug 11 is welded at the bottom end of the movable plug 5, the baffle ring 10 prevents the movable plug 5 from being drawn out, and the rubber plug 11 prevents outside air from entering from the upper end of the liquid storage cylinder 1 and influencing the suction.
Preferably, the supporting plate 3 is in a cross-shaped structure as a whole, the upper connecting piece 6 and the lower connecting piece 8 are in a straight-line structure, two sides of the supporting plate 3 are used for connecting the sleeve 2, and the other two sides are used for supporting so as to facilitate the manual use of the device by a user.
Preferably, the position-limiting area 43 includes a plurality of position-limiting grooves 431 formed on the right surface of the scale post 41, and the height of the position-limiting grooves 431 is greater than the thickness of the positioning block 73, so that the positioning block 73 can be smoothly inserted into the position-limiting grooves 431.
Preferably, the positioning block 73 can rotate 360 degrees at the top end of the supporting column 72, so as to ensure that the movable plug 5 can be completely inserted into the liquid storage cylinder 1.
In the specific using process, firstly, the outer surface of the liquid storage cylinder 1 is held by a hand, the liquid taking port 9 is placed in cell culture fluid infusion, the connecting piece 6 is held by the other hand, the movable plug 5 is extracted from the liquid storage cylinder 1, meanwhile, the measuring mechanism 4 is also extracted from the sleeve 2, the absorption is stopped after a certain amount of fluid infusion is absorbed, the volume of the liquid in the liquid storage cylinder 1 is obtained by observing a scale area of the measuring mechanism, the positioning mechanism 7 is pulled up according to the actual using requirement, the positioning block 73 is inserted into a certain limiting groove 431, then the connecting piece 6 is pressed, the movable plug 5 is pushed towards the inside of the liquid storage cylinder 1 until the connecting piece 71 contacts the lower connecting piece 8, and the reading number of the scale corresponding to the limiting groove 431 in which the positioning block 73 is inserted is subtracted from the reading number of the scale area 43 before the use, so that the amount of the injected liquid can be obtained.
The foregoing shows and describes the general principles, essential features, and advantages of the utility model. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a cell culture is with fluid infusion liquid taking device, includes and deposits liquid section of thick bamboo (1), its characterized in that: deposit a liquid section of thick bamboo (1) lower extreme welding and get liquid mouth (9), deposit a liquid section of thick bamboo (1) with it has lower connecting piece (8) to get liquid mouth (9) junction welding, it has backup pad (3) to deposit a liquid section of thick bamboo (1) upper end welding, lower connecting piece (8) upper surface goes up both sides welding has sleeve (2) that are the symmetry and place, sleeve (2) pass backup pad (3) and weld in backup pad (3), it has movable stopper (5) to deposit a liquid section of thick bamboo (1), every equal sliding connection has measuring mechanism (4) in sleeve (2), movable stopper (5) with measuring mechanism (4) upper end is through last connecting piece (6) fixed connection, the welding has spout (12) on backup pad (3) side surface, sliding connection has positioning mechanism (7) in spout (12).
2. The fluid replacement and extraction device for cell culture according to claim 1, wherein: measuring mechanism (4) including sliding connection in scale post (41) in sleeve (2), set up in scale district (42) of scale post (41) left surface and set up in spacing district (43) of scale post (41) right surface.
3. The fluid replacement and extraction device for cell culture according to claim 1, wherein: positioning mechanism (7) including hug closely and deposit liquid section of thick bamboo (1) outside surface sliding connection's connecting piece (71), weld in support column (72) and the rotation of connecting in connecting piece (71) upper surface both ends connect in locating piece (73) on support column (72) top, support column (72) sliding connection in spout (12).
4. The fluid replacement and extraction device for cell culture according to claim 1, wherein: deposit the welding of the top in the liquid section of thick bamboo (1) and have fender ring (10), the welding of activity stopper (5) bottom has rubber buffer (11).
5. The fluid replacement and extraction device for cell culture according to claim 1, wherein: the whole supporting plate (3) is of a cross structure, and the upper connecting piece (6) and the lower connecting piece (8) are of a straight-line structure.
6. The fluid replacement and extraction device for cell culture according to claim 2, wherein: the limiting area (43) comprises a plurality of limiting grooves (431) formed in the right side surface of the scale column (41).
7. The fluid replacement and extraction device for cell culture according to claim 3, wherein: the positioning block (73) can rotate 360 degrees at the top end of the supporting column (72).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122117852.7U CN215627981U (en) | 2021-09-03 | 2021-09-03 | Liquid supplementing and taking device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122117852.7U CN215627981U (en) | 2021-09-03 | 2021-09-03 | Liquid supplementing and taking device for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215627981U true CN215627981U (en) | 2022-01-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122117852.7U Active CN215627981U (en) | 2021-09-03 | 2021-09-03 | Liquid supplementing and taking device for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215627981U (en) |
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2021
- 2021-09-03 CN CN202122117852.7U patent/CN215627981U/en active Active
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