CN215627956U - Microorganism enrichment device and detection system device for multi-impurity large-sample-size liquid - Google Patents
Microorganism enrichment device and detection system device for multi-impurity large-sample-size liquid Download PDFInfo
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- CN215627956U CN215627956U CN202121747915.0U CN202121747915U CN215627956U CN 215627956 U CN215627956 U CN 215627956U CN 202121747915 U CN202121747915 U CN 202121747915U CN 215627956 U CN215627956 U CN 215627956U
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Abstract
The utility model relates to a little biological enrichment device of big sample volume liquid of many impurities, including prefiltering subassembly and microorganism enrichment subassembly, microorganism enrichment subassembly have included the microorganism enrichment unit of making by hydrophilic fiber material, hydrophilic fiber material's imbibition volume be greater than 5g/g, the gram weight of the microorganism enrichment unit volume that fiber material constitutes is greater than 0.15g/cm3And the detection efficiency of the microorganism enrichment device is more than 1200 s/10L. Enrichment device of the present disclosurePut the detectable rate that can show ground improvement target microorganism, and use the cotton absorbent gauze as the filter element layer and can let device reuse, conveniently change inside filter element layer structure, reduce use cost.
Description
Technical Field
The disclosure relates to the technical field of microorganism enrichment and detection, in particular to a microorganism enrichment device and a detection system device for multi-impurity large-sample-volume liquid.
Background
Food safety is related to public health and life safety, and national importance on food safety is raised to a legislative level, wherein food-borne pathogenic bacteria are one of the most important food safety risks. Common food-borne pathogenic bacteria include salmonella, pathogenic escherichia coli, staphylococcus aureus and the like. The food-borne pathogenic bacteria mainly take food as a transmission medium, can enter a host and generate pathogenicity to cause food poisoning.
Pathogenic bacteria residues on food materials are the main ways for food-borne pathogenic bacteria to enter hosts, and the pathogenic bacteria residues exist in uncooked vegetables which are not cleaned; food materials do not kill food-borne pathogenic bacteria in the cooking process, and pathogen residues exist; pathogen residues are caused by pollution of an edible water source, pollution in the process of cultivating food materials and the like. Therefore, how to detect the food-borne pathogens is directly related to the food safety of the public, and the rapid detection of the food-borne pathogens is mainly carried out through the water sources related to the food materials, including the water source for food material cultivation irrigation and the existence of pathogens in the water source after the food materials are washed, so as to judge whether the food materials are safe or not.
At present, for uncooked fruits and vegetables, detection standards for microbial items are not established, most of the detection standards refer to detection standards for pathogenic bacteria items in prepackaged food, and the specific operation is to collect edible parts of 25g of fruit and vegetable samples and perform enrichment culture. Due to the small sampling amount, the real situation of pathogenic microorganisms in the uncooked fruits and vegetables is difficult to embody. The detection of farmland irrigation water, environmental water sources and the like is carried out by referring to the detection standard GBT5750.12-2006 of domestic water and the detection standard GB8538-2016 of packaged drinking water, the detection standards all disclose detection methods of microorganisms in liquid, most specific operations are quantitative water sampling for enrichment culture, and the water sampling is selected to be 1mL or 10 mL; or after filtering by using a filtering membrane, enriching the microorganisms and then culturing, wherein the water sample before filtering is about 100mL or 250mL, and the maximum amount is not more than 250mL generally. Furthermore, conventional sampling techniques for the detection of microorganisms in ambient water require the water sample to be kept in refrigerated transport, thus often limiting the actual number of samples that can be transferred to laboratory testing.
The detection standard disclosed at present provides a detection method for microorganisms in a water source, but the detection method has the problems of inaccurate detection and unobtrusive result. These are mainly due to the state of the microorganisms in the water sample, and because the distribution of the microorganisms is not necessarily uniform when the microorganisms exist in the water, and the sampling amount of the current detection method is small, during the sampling process, the taken water sample does not necessarily contain the target microorganisms, but the water sample containing the target microorganisms is likely to be missed for detection, so that the detection result is greatly different from the result in the real water source. In order to reduce detection errors, more times of sampling, repeated detection and comprehensive experimental data are needed, and the detection means is extremely complex and takes long time for detection; meanwhile, even if the sampling is carried out for multiple times, the sampled specimen still has certain limitation, and a false negative result is easily caused.
For the filtration method of enriching bacteria by a filtration membrane, although the filtration method is used: the method is characterized in that a water sample is filtered to enrich bacteria, but due to the performance of a microbial membrane material, pores are small and are distributed uniformly, the pores on a biological membrane are extremely easy to block, so that the filtering efficiency is low, and meanwhile, the negative pressure required during filtering is overlarge, so that the severe filtering condition easily causes the morphological variation and death of microorganisms, so that the detection result is inaccurate, and therefore, the method for filtering the membrane is not suitable for liquid samples with multiple impurities; and the water sample is also selected in the specifications of 100mL and 250mL, so that the method is not suitable for detecting large-sample-volume liquid.
At present, the detection of food-borne pathogenic bacteria in the food field comprises pathogen detection of water sources before and after the planting process and cleaning of raw fruits and vegetables, the water source to be detected is complex relative to the components of drinking water, and a large amount of silt, fruit and vegetable juice leaves and the like may exist in liquid to be detected, so that the problems of repeated sampling, complex detection, long detection time consumption, large difference between a detection result and a real result and the like can be derived by simply using the detection method mentioned in the national standard.
Based on the problems of the existing detection methods for microorganisms in liquid, especially for the detection method for liquid to be detected containing complex components and multiple impurities, a need is urgently needed to develop a new detection device and a new detection method, and to accurately detect the number and the types of microorganisms in the liquid to be detected through the device and the detection method.
SUMMERY OF THE UTILITY MODEL
The microorganism enrichment device has the advantages of large sampling amount, high microorganism enrichment speed, simplicity and easiness in operation, small size, convenience in carrying and transportation, low cost, environmental friendliness, reusability and the like. Through increasing liquid sample volume, control filter effect and bacterium simultaneously and catch the enrichment effect, provide more accurate information for the quality of water that agricultural product was irrigated and vegetables were washd, be used for assessing the better instrument of various water source risks.
The conception of the disclosure lies in the improvement of the filtering unit of the device, the filtering unit can ensure that the liquid with a plurality of impurities and a large sample amount is filtered, and the filtering process does not occur or slightly occurs blockage, the filtering unit can accurately capture the microorganisms in the sample to be detected, and the death of the microorganisms is effectively reduced.
In order to achieve the above object, the present disclosure provides the following technical solutions:
an apparatus for enriching microorganisms in a large sample volume of liquid, the apparatus comprising:
a pre-filtration component and a microorganism enrichment component;
the microorganism enrichment assembly is used for carrying out secondary filtration on the filtrate filtered by the pre-filtering assembly and adsorbing and enriching microorganisms in the filtrate;
the pre-filtering component comprises a filter screen;
the filter screen is mainly used for filtering large-particle impurities in liquid to be detected in the using process, such as organic saprophytes, insects, duckweeds and the like possibly existing in irrigation water, food residues, sand and stones and the like in the liquid to be detected after the food is cleaned. And further, impurities with large volume in the liquid to be detected are greatly reduced, and the influence on the enrichment of microorganisms and the filtering efficiency caused by the blockage of the microorganism enrichment assembly by the impurities with large volume is avoided.
The present disclosure does not specifically limit the screen structure, materials, and location of the pre-filter assembly;
the filter screen is a mesh structure with pores, wherein the shape of the pores comprises a circle, a triangle, a quadrangle, a pentagon, a hexagon, an octagon and the like;
further, the diameter of the pore is more than 1 mm;
the filter screen is made of metal and/or non-metal materials;
the metal material comprises iron, copper, aluminum and the like, and the non-metal material is an organic polymer material, such as polyester, PP, PBS, polyethylene, polypropylene and the like.
The pore of the filter screen structure of the pre-filtering component is far larger than the size of bacteria, so that the bacteria cannot be intercepted and adsorbed in the pre-filtering process.
The microorganism enrichment component of the disclosed device comprises a microorganism enrichment unit composed of fiber materials;
the microorganism enrichment component utilizes fiber materials as filling filter materials, and mainly utilizes the characteristics of porosity, air permeability, fiber materials and the like of the fiber materials to intercept and enrich microorganisms.
On the one hand, the position is intricate complicated among the fibre in the fibrous material, and the pore form that forms is different, just because of this kind of special pore structure, can withhold the little microorganism of volume to fix the microorganism between the fibre, simultaneously because the existence in hole, also be easy with the quick discharge of moisture, and then can carry out the microorganism enrichment to bulky sample.
On the other hand, the fibers in the fiber material are generally flexible materials, the structure of the fibers can be slightly changed under the condition of high pressure or high flow rate of liquid, so that the fiber material is suitable for the rapid passing of the liquid, most of the fiber material in the liquid (water) has negative charges, and pores formed among the fibers are not easy to block, so that the fiber material can ensure the filtering effect and simultaneously has higher interception and enrichment effects on microorganisms.
The fiber material is a hydrophilic fiber material;
the hydrophilic fiber material is one or more of cotton fiber, wool fiber, silk fiber, seaweed fiber and hydrophilic modified synthetic fiber;
in some embodiments, the fibrous material is a woven fabric;
more particularly, to woven fabrics comprised of hydrophilic fibrous materials.
The textile fabric is woven, tatted, knitted and/or non-woven fabric.
The liquid absorption of the textile fabric material is more than 5g/g, preferably more than 6g/g, more preferably more than 8 g/g;
furthermore, the liquid absorption of the textile fabric material is less than 20 g/g; more preferably less than 10 g/g.
Wherein the test liquid of the liquid suction amount is water;
the fibers forming the textile fabric selected for use are hydrophilic fibers, the hydrophilic fibers mostly have groups such as hydroxyl groups and amino groups which can form hydrophilic bonds with water, water molecules can be fastened in the fibers, and the fibers can expand to a certain extent after meeting water, so that the pores between the fibers and the fibers in the textile material are reduced, and the purpose of filtering microorganisms is achieved.
In addition, the liquid absorption amount of the textile fabric needs to be in a reasonable range to achieve the interception and filtration of microorganisms, if the liquid absorption amount of the fabric is small, namely the hydrophilicity of the fibers is poor, the shape change of the fabric after water absorption is not obvious (the fibers do not swell), the pores among the fibers are difficult to reduce, and the interception effect of the textile fabric material on the microorganisms is poor; or, when the liquid absorption amount of the fiber fabric is too large, for example, the liquid absorption amount is more than 20g/g, the shape of the fiber after absorbing the liquid is difficult to keep stable, and in the filtering process of high pressure and high flow rate, the fiber is easily taken away by the liquid of high pressure and high flow rate, so that the pores of the textile fabric are changed violently, and the filtering performance is lost.
In some embodiments, the textile fabric materials used in the present disclosure are negatively charged materials in a liquid (water).
The material with negative charges in the liquid is specifically selected, the material and the microorganism have the same charge condition in the liquid, and the cell wall of the microorganism cannot deform due to the charge in the filtering process, so that the survival rate of the microorganism is improved, and the detection accuracy is improved.
In some embodiments, the microorganism enrichment assembly comprises a housing having a cylindrical configuration;
in the column structure of the present disclosure, the shape of the column structure is not particularly limited, and includes a cylindrical shape, a square column shape, and other irregular column structures.
The space formed by the shell with the cylindrical structure is used for filling the microorganism enrichment unit formed by the fiber material.
The length of the shell of the cylindrical structure along the axial direction is not less than 100mm, preferably not less than 140 mm;
as is well known, in the enrichment of microorganisms in a liquid, since the volume of the microorganisms to be enriched is small, generally about 0.5 μm, conventionally, we generally consider that the pore size of a filter material is made to be less than or equal to 0.5 μm, such as a common filter membrane, which has pores less than or equal to 0.5 μm, and can filter and enrich microorganisms efficiently, but the cost of the microbial filter membrane is too high, and the amount of filtered samples is small. In actual operation, the liquid to be measured not only contains microorganisms, but also contains other impurities in most cases, so the microorganism filtering membrane is extremely easy to block, and a large negative pressure is needed in the filtering process to filter the liquid, and the volume of the liquid sample to be measured is generally less than 250mL by a common method for filtering and enriching by using the filtering membrane. On the other hand, the pore diameter of the microorganism filtering membrane is small, and the negative pressure in the filtering process is large, so that the form of a cell membrane of the microorganism is extremely easy to damage in the filtering process, the microorganism is dead, and the type and the quantity of the microorganism to be detected cannot be accurately measured in the subsequent identification and detection processes.
Aiming at the defects of the existing microorganism enrichment filtration, the novel design is carried out on the filtration enrichment component, the microorganism enrichment component is set to be of a cylindrical structure, the length of the cylindrical structure shell along the axial direction is not less than 100mm, and the microorganism enrichment effect is improved by prolonging the filtration path. The design of the long filtering path does not require that the pores of the microorganism enrichment unit are smaller than the volume of the microorganisms (relative to the filter membrane), and meanwhile, the pore size structure of the filtering is complex (not all linear filtering pores) by specifically matching with the fiber material microorganism enrichment unit selected by the disclosure, and the microorganisms can be rapidly adsorbed and fixed by utilizing the capillary effect and the adsorption performance of the pores. Simultaneously because the size in hole can be suitable for enlargiing, also make the microorganism enrichment facility of this disclosure can be applicable to quick detect to big sample liquid.
In some embodiments, the microbial enrichment unit of dimensional material is disposed within the housing of the cylindrical structure;
the gram weight of the microorganism enrichment unit volume formed by the fiber material is more than 0.15g/cm3(ii) a Preferably greater than 0.2g/cm3(ii) a More preferably greater than 0.25g/cm3；
Furthermore, the gram weight of the whole fiber material biological enrichment component is more than 50g, preferably more than 60 g; more preferably greater than 70 g.
In the microorganism enrichment process, the microorganism is fixed through a long filtering path and complicated filtering pores, and the tightness of the fiber material in the microorganism enrichment component is influenced, wherein the larger the tightness, the smaller the pores are, but the filtering efficiency is reduced; the smaller the degree of compaction, the larger the pores, and the less the filtration effect, but the higher the filtration efficiency. The overall mass of the microorganism enrichment assembly, which determines the cross-sectional area of filtration, also affects filtration efficiency.
Furthermore, the radial circumference of the cylindrical shell is not less than 140mm, and preferably not less than 180 mm.
In some embodiments, the microorganism enrichment assembly further comprises an end cap;
the end cover is detachably connected with the shell and has a sealing design;
the connection mode comprises threaded connection and/or buckling connection;
the end cover is used for fixing the microorganism enrichment unit in the cylindrical shell, and the end cover can be arranged to ensure that the microorganism enrichment unit is convenient to disassemble.
In some embodiments, the prefilter module is connected to a microorganism enrichment module.
The unit for connecting the pre-filtering component and the microorganism component is a tubular component;
the length and the specific shape of the tubular assembly are not particularly limited, and the tubular assembly is used for conveying the liquid filtered by the pre-filtering assembly to the microorganism enrichment assembly for subsequent secondary filtration.
In some embodiments, the microorganism enrichment assembly comprises a second liquid stream outlet;
the second liquid outlet is connected with a negative pressure device;
the negative pressure device is one of a water pump and a vacuum pump;
in some embodiments, the microorganism enrichment device has a detection efficiency of greater than 1200 s/10L; more preferably more than 600 s/10L; more preferably more than 400 s/10L.
The liquid for detection efficiency was 10L of water.
In some embodiments, the pre-filter assembly comprises a pre-filter support;
the specific structure of the pre-filtering bracket is not limited, and the pre-filtering bracket is used for fixing or connecting;
in some embodiments, the prefilter holder includes a first liquid flow outlet for securing a tubular assembly;
in some embodiments, the microorganism enrichment assembly comprises a liquid stream inlet for connection to a tubular assembly;
in some embodiments, the present disclosure selects a textile fabric made of hydrophilic fibers as the microorganism enrichment unit;
the liquid absorption of the textile fabric is 5-10 g/g;
the gram weight of the microorganism enrichment unit of the textile fabric is 0.15-0.3g/cm3；
The gram weight of the microorganism enrichment unit is 50-70 g;
the microorganism enrichment assembly is cylindrical, the length of the cylindrical structure is more than 100mm, and the circumference is more than 100 mm;
the detection efficiency of the microorganism enrichment device is 200-1200 s/10L.
In some embodiments, the present disclosure selects a textile fabric made of hydrophilic fibers as the microorganism enrichment unit;
preferably, the hydrophilic fiber is absorbent cotton fiber;
the liquid absorption amount of the textile fabric made of the absorbent cotton is 5 g/g;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the absorbent cotton is 0.26g/cm3；
The gram weight of the microorganism enrichment unit is 67 g;
the cylindrical structure of the microorganism enrichment assembly is cylindrical, the length of the cylinder is 145mm, and the circumference is 182 mm;
in the embodiment, the detection efficiency is 612s/10L, and the liquid sample to be detected with the volume of 10L containing 100 target pathogenic bacteria can be accurately detected.
In some embodiments, the present disclosure also provides a detection system using the aforementioned microorganism enrichment device, the detection system comprising the aforementioned microorganism enrichment device and a negative pressure device;
the negative pressure device is a vacuum pump and/or a water pump.
The utility model provides a microorganism enrichment device is including prefiltering subassembly and microorganism enrichment subassembly, and the microorganism enrichment unit that microorganism enrichment subassembly used chooses for use fibrous material to replace traditional microbial membrane to filter, can be directed against the bulky, and the liquid that awaits measuring of many impurities detects, detects accuracy and detection efficiency and greatly improves, and microorganism enrichment device can reuse simultaneously, low in manufacturing cost, convenient to use.
The detection method of the microorganism enrichment device is different from the traditional detection method, can detect a large amount of liquid to be detected, can detect a liquid sample with a volume of at least 10L at one time, can detect a detection object such as water (environmental water samples such as irrigation water, rivers, lake water and the like) or fresh fruit and vegetable leacheate and the like, can successfully capture target microorganisms in a large-scale water body or a large amount of fruits and vegetables, and can obviously improve the detection rate and accuracy of the target microorganisms.
The microorganism enrichment device disclosed by the invention is simple and convenient to sterilize, key parts can be subjected to high pressure, the consumable material of the device is only a microorganism enrichment unit, namely a textile fabric, the cost is low, the replacement is convenient, and after the device is used, conventional biological safety treatment (such as high-pressure sterilization) can be carried out along with bacterium-enriched broth without special treatment.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present disclosure, and other drawings can be obtained by those skilled in the art according to the drawings.
Fig. 1 is a schematic structural diagram of a microorganism enrichment device for a multi-impurity large-sample-volume liquid according to an embodiment of the present disclosure;
FIG. 2 is a schematic structural diagram of a microorganism enrichment device for a multi-impurity large-sample-volume liquid according to another embodiment of the present disclosure;
fig. 3 is a schematic structural diagram of a microorganism enrichment device for a multi-impurity large-sample-volume liquid, which is disclosed as a further embodiment.
Description of reference numerals:
1. a pre-filter assembly; 2. a microorganism enrichment assembly; 3. a tubular assembly;
101. pre-filtering the stent; 102. filtering with a screen; 103. filtering holes; 104. a first liquid flow outlet;
201. a housing; 202. a microorganism enrichment unit; 203. an end cap; 204. a liquid flow inlet; 205. A second liquid outflow port.
Detailed Description
In order to make the technical solutions of the present disclosure better understood by those skilled in the art, the present disclosure will be described in further detail below with reference to the accompanying drawings.
One embodiment of the present disclosure discloses a microorganism enrichment device for a multi-impurity large sample volume liquid, which is shown in fig. 1;
the enrichment device comprises a pre-filtering component 1; and
a microorganism enrichment assembly 2;
the pre-filtering component 1 is connected with the microorganism enrichment component 2;
the connection can be integrated connection or detachable connection;
the pre-filtering component 1 comprises a filter screen;
the filter screen is arranged in the radial direction of the pre-filtering component;
the filter screen in this embodiment is a conventional filter screen, which is used to pre-filter large particulate matter present in the fluid, such as sand, fruit and vegetable debris, etc., to prevent the large particulate matter from blocking the liquid inlet of the device.
The enrichment assembly comprises a cylindrical shell 201; the length of the shell along the axial direction is 145 mm; the radial circumference of the cylindrical structural shell is 182 mm.
The cylindrical shell is internally provided with a microorganism enrichment unit.
The microorganism enrichment assembly further comprises an end cover 203, wherein the end cover can seal the shell and hermetically fix the microorganism enrichment unit in the cylindrical shell.
And a liquid outlet is arranged on the end cover and used for guiding out the liquid after the second filtration.
In another embodiment of the present disclosure, referring to fig. 2, the filter screen is disposed on a sidewall of the pre-filter assembly;
still another embodiment of the present disclosure discloses a microorganism enrichment apparatus for a multi-impurity large sample volume liquid, as shown in fig. 3, the enrichment apparatus comprising:
a pre-filter assembly 1; and
the microorganism enrichment assembly 2 is positioned on the pre-filtering assembly 1 and is communicated with the pre-filtering assembly 1;
the pre-filtering component 1 is provided with a metal filter screen 102 for isolating large-particle impurities, and liquid pre-filtered by the metal filter screen 102 enters the pre-filtering component 1;
the microorganism enrichment assembly 2 receives the fluid output by the pre-filtering assembly 1 and pre-filtered by the metal filter screen 102;
the microorganism-enriching assembly 2 has a microorganism-enriching unit 202 inside.
Specifically, the embodiment discloses a multi-impurity liquid sample microorganism enrichment device with a secondary filtering structure, which is respectively provided with a pre-filtering component 1 and a microorganism enrichment component 2; the pre-filtering assembly 1 is mainly used for pre-filtering large particulate matters such as sand, fruit and vegetable debris and the like in a fluid to prevent the large particulate matters from blocking a liquid inflow port of the device, and the large particulate matters can be completely isolated by using the filter screen 102 of the pre-filtering assembly 1 to prevent the large particulate matters from entering the device;
the material of the screen 102 may be metallic or non-metallic;
the aperture of the filtering hole 103 of the filter screen 102 is 0.5 mm.
Next, the prefilter module 1 is connected to a microorganism enrichment module 2, and the microorganism enrichment module 2 of the present embodiment mainly enriches microorganisms and is structured as a microorganism enrichment unit 202, which is made of hydrophilic fiber material.
Specifically, the microorganism enrichment unit 202 is a woven fabric made of hydrophilic fiber material.
The microbial filtration unit that pure weaving fabric of this embodiment constitutes not only can handle the many impurity liquid of big sample volume (10L), can also fully intercept, enrichment microorganism, and simultaneously, the convenient change of pure cotton absorbent gauze lets the device reuse nature good.
In some embodiments, referring to fig. 3, the pre-filter assembly 1 comprises:
a support 101; and
a filter screen 102 is arranged along the circumferential direction of the bracket 101, and the metal filter screen 102 is a stainless steel filter screen;
the pre-filtering assembly 1 is formed into a cylindrical structure with a hollow interior by a pre-filtering bracket 101 and a metal screen 102;
the end of the pre-filtering component 1 close to the microorganism enrichment component 2 is provided with a first liquid outlet 104;
the surface of the metal screen 102 is uniformly provided with filter holes 103 communicated with the interior of the pre-filter assembly 1, and the fluid enters the pre-filter assembly 1 through the filter holes 103 and is conveyed to the microorganism enrichment assembly 2 through the first liquid outlet 104.
Wherein, the length of the pre-filtering component 1 is 40mm, and the diameter of the pre-filtering component 1 is 28 mm;
the outer diameter of the first liquid flow outlet 104 was 10 mm.
In some embodiments, the microorganism enrichment assembly 2 comprises:
a cylindrical structure housing, wherein the housing may be a cylindrical housing 201;
the end of the shell close to the pre-filtering component 1 is provided with a liquid inflow port 204 and is connected with the microorganism enrichment component 2 through a tubular component 3.
The liquid after the pre-filtration is conveyed to the microorganism enrichment component 2 through the tubular component to intercept and enrich the target microorganisms.
In some embodiments, the microorganism enrichment assembly 2 further comprises an end cap 203;
the end cap can be arranged at one end of the microorganism enrichment assembly shell, or both ends are simultaneously provided with the end cap 203;
the end cover is provided with holes for liquid to flow through.
Meanwhile, the end cover 203 can fix the microorganism enrichment unit;
the end cap 203 is detachably connected with the housing through a thread and/or a snap structure.
Specifically, a liquid inlet 204 is arranged on the end cover 203 near one end of the pre-filtering assembly; a second liquid flow outlet 205 is provided in the end cap remote from the end of the pre-filter assembly 1.
In some embodiments, the microorganism filtration unit 202 of the microorganism enrichment assembly 2 is a woven fabric made of hydrophilic fiber material;
the microorganism filtering unit 202 is arranged in the shell 201 with a cylindrical structure, and the microorganism enriching unit 202 is fixed through the end cover 203.
In some embodiments, the microorganism enrichment unit 202 is one or more of absorbent cotton gauze, alginate fiber gauze, absorbent cotton non-woven fabric, alginate/absorbent cotton blended gauze, absorbent cotton knitted fabric, alginate fiber woven fabric, carboxymethyl cellulose gauze, hydrophilic polyester gauze, cotton/wool blended gauze.
The disclosed embodiment defines the structure and principle of action of the pre-filter assembly 1; the pre-filtering component 1 mainly comprises a pre-filtering bracket 101 and a metal filter screen 102, wherein the metal filter screen 102 is fixed on the wall of the pre-filtering component 1, and the metal filter screen 102 is a part which is mainly used for isolating large granular substances and fluid from entering the device. The metal screen 102 is the main large particle impurity filtering component. When designed, the pre-filter assembly 1 may be a closed structure at one end and a first liquid stream outlet 104 connected to the microorganism enrichment assembly 2 downstream of the process at the other end. The fluid filtered by the screen 102 enters the microorganism enrichment module 2 downstream of the process through the pre-filter module first liquid stream outlet 104 for microorganism enrichment. The multi-layer filtering concept can ensure that the microorganism enrichment unit is not blocked by larger impurities.
The above dimensions of the present embodiment are merely exemplary to explain and illustrate the product structure, which can be adjusted appropriately according to the actual process requirements.
The present disclosure also provides another embodiment, a detection system using the microorganism enrichment apparatus, the detection system including the microorganism enrichment apparatus and a negative pressure apparatus;
the negative pressure device is a vacuum pump and/or a water pump.
The negative pressure device can generate enough negative pressure, and the microorganism enrichment device can be used for carrying out high-efficiency target microorganism enrichment on liquid with multiple impurities and large sample size.
Preferably, in some embodiments, a detection system using the aforementioned microorganism enrichment apparatus is disclosed, wherein the biological enrichment assembly 2 comprises:
a cylindrical housing 201; and
a microorganism enrichment unit 202 within the housing 201;
both ends of the microorganism enrichment component 2 are sealed by stainless steel end covers 203;
the filter assembly nozzle on the side close to the pre-filter assembly 1 is configured as a liquid inflow port 204 and the filter assembly nozzle on the side remote from the pre-filter assembly 1 is configured as a second liquid outflow port 205.
Wherein the first liquid outlet 104 of the pre-filter assembly is connected to the liquid inlet 204 via the tubular assembly 3;
the second liquid outflow 205 of the module 2 for enrichment of microorganisms is connected to a water pump downstream of the process through the tubular module 3.
The length of the shell 201 in this embodiment is 145mm, and the radial circumference of the shell is 182 mm;
the liquid inlet 204 and the second liquid outlet 205 are both stainless steel pipes, the length of each stainless steel pipe is 40mm, and the outer diameter of each stainless steel pipe is 9.5 mm;
the center of the stainless steel end cover 203 is provided with a through hole of 8.5mm for leading in and leading out liquid.
The structure of the microorganism enrichment assembly 2 is defined in detail in the embodiment, firstly, the main structure of the microorganism enrichment assembly is a shell 201, the shell 201 provides an embedded space for the microorganism enrichment unit 202 inside, and the shell is closed by end covers 203 at two ends, so that the structure is simple, and the replacement and maintenance are convenient.
It should be noted that, because the prefiltering assembly 1 and the microorganism enriching assembly 2, and the microorganism enriching assembly 2 and the water pump are connected by the tubular assemblies 3, the length and the pipe diameter of the tubular assemblies 3 can be adjusted according to actual process requirements and sampling requirements.
Further, the microorganism enrichment unit 2 is absorbent cotton gauze, and the whole mass of the absorbent cotton gauze is 51 g;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the absorbent cotton gauze is 0.19g/cm3；
The filtration efficiency is 207 s/10L.
In this example, the retention of microorganisms was 54.7%
Further, the absorbent cotton gauze is used; the whole weight of the degreased gauze cloth is 59 g;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the absorbent cotton gauze is 0.23g/cm3；
The filtration efficiency is 340 s/10L.
In this example, the retention of microorganisms was 66.9%.
Further, the absorbent cotton gauze is used; the whole weight of the degreased yarn cotton cloth is 67 g;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the absorbent cotton gauze is 0.25g/cm3；
The filtration efficiency is 612 s/10L.
In this example, the retention rate of microorganisms was 77.3%.
Further, the microorganism enrichment unit 2 is alginate fiber gauze, and the overall mass of the alginate fiber gauze is 61 g;
the liquid absorption amount of the alginic acid fiber gauze is 12g/g, wherein the liquid is water;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the alginate fiber gauze is 0.23g/cm3；
The filtration efficiency is 305 s/10L.
In this example, the retention of microorganisms was 65.7%
Further, the microorganism enrichment unit 2 is a cotton knitted fabric, and the overall mass of the cotton knitted fabric is 60 g;
the liquid absorption amount of the absorbent cotton gauze is 5g/g, wherein the liquid is water;
the gram weight of the microorganism enrichment unit volume of the textile fabric made of the absorbent cotton gauze is 0.23g/cm3；
The filtration efficiency is 210 s/10L.
In this example, the retention of microorganisms was 57.7%.
It should be noted that, in the method for testing the retention rate of microorganisms in the embodiment of the present disclosure, a certain number of microorganisms are placed in a liquid to be tested, then the device disclosed in the embodiment of the present disclosure is used to enrich the microorganisms, finally, the microorganism enrichment unit is eluted, the eluate is diluted and then plated on a flat plate for overnight culture, the number of bacterial colonies is observed and counted, and then the retention rate of the microorganisms is calculated.
In contrast, with the negative pressure device (i.e., the water pump) of the detection system using the aforementioned microorganism enrichment device provided in the previous embodiment, the time for separately drawing 10L of the sample was 181 s;
in addition, as a comparison, by using the detection system using the aforementioned microorganism enrichment device provided in the previous embodiment, the microorganism enrichment assembly 2 does not include the microorganism enrichment unit 202, and a negative pressure device is used to draw 10L of liquid sample for 189 s.
In addition, by contrast, with the detection system using the aforementioned microorganism enrichment device provided in the previous embodiment, the microorganism enrichment unit 202 in the biological enrichment assembly 2 is replaced by a microorganism film, so as to ensure the immobilization of the film;
for this comparison, the pumping filtration time was much greater than 2000 s; meanwhile, the structure of the microbial filtration membrane is damaged by naked eyes, and the filtration result cannot be detected.
Preferably, in one embodiment the expanded size of the pure cotton absorbent gauze is 1300 x 840 mm;
a microorganism enrichment unit 202 which is made by folding and rolling pure cotton absorbent gauze.
Wherein, the folding mode of above-mentioned pure cotton absorbent gauze does:
folding the short edge once, and then dividing the short edge into trisection folding to form a gauze strip with the size of 1300 x 140 mm;
the gauze strip is folded twice and then curled into a biological enrichment unit 202 with the diameter of about 50 mm.
The enrichment apparatus disclosed in this embodiment can capture planktonic cells, fine environmental sludge, and cohesive sediments etc. (typically 8 to 24 μm) to which target pathogenic microorganisms adhere.
In some embodiments, the microorganism enrichment assembly 2 in the microorganism enrichment apparatus can be used alone, mainly for a fluid (such as irrigation water) without large particulate matters inside, the microorganism enrichment assembly 2 can intercept and enrich pathogenic microorganisms by using alone, and for a fluid carrying large particulate matters, the fluid needs to be treated by the two layers of the pre-filtration assembly 1 and the microorganism enrichment assembly 2.
In other embodiments, the present disclosure also provides a detection method using the microorganism enrichment device provided above;
the method comprises the following steps:
1) placing the microorganism enrichment device in a liquid to be detected;
2) and (3) communicating the microorganism enrichment device with a negative pressure device, and opening the negative pressure device to pump water.
3) And after the liquid to be detected is filtered, closing the negative pressure device, and taking out the microorganism enrichment unit in the microorganism enrichment assembly.
4) Directly putting the taken microorganism enrichment unit into a homogeneous bag filled with corresponding enrichment broth for carrying out target microorganism culture, and carrying out separation and identification on the target microorganism according to a standard method after enrichment.
In some embodiments, the present disclosure uses the microorganism enrichment device obtained from the above disclosure and the provided detection method to detect an actual sample;
the device is carried to 3 public garden lakes and 1 city river for suction filtration sampling of 10L of water, and simultaneously, two 250ml water samples are additionally collected at each sampling point and brought back to a laboratory for direct culture and detection. As a result: by adopting the device to pump and filter 10L of water for sampling, salmonella of food-borne pathogenic bacteria is detected at 2 of 4 sampling points to be positive, and the detection results of 8 250ml water samples collected by 4 sampling points of a control group are negative.
As can be seen from the actual test results, the device used in the present disclosure can detect objective results, and the detection accuracy is more reliable compared with the sampling method.
In above-mentioned technical scheme, the microorganism enrichment facility of big sample volume liquid of many impurities that this disclosure provided has following beneficial effect:
this disclosed enrichment device divide into prefiltration subassembly 1 and microorganism enrichment subassembly 2 according to fluidic circulation technology route, and microorganism enrichment subassembly 2 utilizes hydrophilic fiber material as microorganism enrichment unit 202, can show and improve the relevance ratio of target microorganism, separates the living target microorganism that can cultivate, and can let device reuse as the filter element layer with pure cotton absorbent gauze, conveniently changes inside filter element layer structure, reduces use cost.
The enrichment device of the present disclosure provides a novel method for detecting multiple contaminant liquid samples, which is an economical and practical method for assessing pathogenic microorganisms that may be present in environmental water, irrigation water, and raw fruit and vegetables by collecting a large number of samples. The device has large detection amount, can detect large-volume liquid samples of more than 10L or more than 10L at one time, can detect complex liquid samples with multiple impurities, such as water (farmland irrigation water, environmental water samples such as underground water, rivers, lakes and the like) or leacheate of fresh fruits and vegetables, can successfully capture target microorganisms in large-range water or a large amount of fruits and vegetables, and remarkably improves the content of multiple impurities and the large amount of liquid samplesDetecting the detection rate of target pathogenic microorganisms in the sample amount of liquid; the detection precision is high, and 10L of liquid to be detected containing 10 can be detected0Concentration of individual target strains.
The enrichment device disclosed by the invention is simple and convenient to sterilize, key parts can be sterilized under high pressure, and the microorganism enrichment unit 202 is made of a fiber material, so that the cost is low, the sensitivity is high, and the device can be recycled.
The enrichment device disclosed by the invention can be carried to the outdoor for sampling water environment or farmland irrigation water, and can also be used in a laboratory for enriching target microorganisms of raw fruit and vegetable leacheate.
While certain exemplary embodiments of the present disclosure have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present disclosure. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the disclosure.
Claims (8)
1. The microorganism enrichment facility of many impurity large sample volume liquid, its characterized in that, this enrichment facility includes:
a microorganism enrichment assembly (2);
the microorganism enrichment assembly (2) comprises a microorganism enrichment unit (202);
the microorganism enrichment unit (202) is made of a hydrophilic fiber material;
the liquid absorption amount of the hydrophilic fiber material is more than 5 g/g;
the gram weight of the unit volume of the microorganism enrichment unit consisting of the hydrophilic fiber material is more than 0.15g/cm3；
The microorganism enrichment assembly comprises a shell with a cylindrical structure;
the microorganism enrichment assembly comprises an end cap;
the end cover is connected with the shell and can be detached;
the space formed by the shell of the cylindrical structure is used for filling a microorganism enrichment unit (202);
a pre-filter assembly (1);
the pre-filtering component (1) is communicated with the microorganism enrichment component.
2. The microbial enrichment device of a multiple impurity bulk liquid according to claim 1, wherein the pre-filter assembly (1) comprises a filter screen; the filter screen is used for intercepting large granule impurity.
3. The apparatus for the microbial enrichment of a multiple impurity bulk liquid according to claim 1,
the columnar structure is one of a cylindrical structure, a square column structure or other irregular columnar structures.
4. The apparatus for the microbial enrichment of a multiple impurity bulk liquid according to any one of claims 1 to 3,
the hydrophilic fiber material is one or more of cotton fiber, wool fiber, silk fiber, seaweed fiber and hydrophilic synthetic fiber;
the hydrophilic fiber material is made into a textile fabric;
the textile fabric is woven, tatted, knitted and/or non-woven fabric;
the textile fabric made of the hydrophilic fiber is used as a microorganism enrichment unit.
5. The microbial enrichment device of a multiple impurity bulk liquid according to any one of claims 1-3, wherein the microbial enrichment module (2) comprises an end cap (203);
the end cover (203) is detachably connected with the shell.
6. The microbial enrichment device of a multi-impurity bulk liquid according to any one of claims 1 to 3, wherein the pre-filter assembly (1) is in communication with the microbial enrichment assembly (2) via a tubular assembly (3);
a first liquid outlet (104) is arranged on the pre-filtering assembly;
the microorganism enrichment assembly is provided with a liquid inflow port (204) and a second liquid outflow port (205);
said tubular assembly being connected at one end to a first liquid flow outlet (104); the other end is connected with a liquid inflow port (204).
7. A microorganism enrichment detection system device for a multi-impurity large sample volume liquid, which is characterized in that the microorganism enrichment device for the multi-impurity large sample volume liquid according to any one of claims 1 to 6 is used;
the microorganism enrichment assembly is communicated with the negative pressure device;
the negative pressure device is a vacuum pump or a water pump.
8. The system for the microbiological enrichment test of a multiple impurity bulk liquid of claim 7, wherein the detection efficiency of the microbiological enrichment device is greater than 1200 s/10L.
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