CN109762722A - A kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable - Google Patents
A kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable Download PDFInfo
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- CN109762722A CN109762722A CN201910086807.4A CN201910086807A CN109762722A CN 109762722 A CN109762722 A CN 109762722A CN 201910086807 A CN201910086807 A CN 201910086807A CN 109762722 A CN109762722 A CN 109762722A
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- microorganism
- filter membrane
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Abstract
The present invention relates to a kind of Double-filtering devices for microorganism-collecting in fresh-cut fruit and vegetable, belong to microorganism acquisition equipment technical field.The device includes: fruits and vegetables homogeneous collection, large aperture filter membrane, bacterium solution collector, small-bore filter membrane and filtrate displacer, above each component is according to being from top to bottom sequentially arranged, large aperture filter membrane is polypropylene material or nylon material, and small-bore filter membrane is nylon material.Each component of the present invention is detachable, is convenient to clean and samples, and simplifies the step of collecting microorganism, improves the quantity for collecting microorganism, and component is detachably also beneficial to the replacement of strainer, adapts to the requirement that different microorganisms are collected.
Description
Technical field
The present invention relates to a kind of Double-filtering devices for microorganism-collecting in fresh-cut fruit and vegetable, belong to microorganism acquisition and set
Standby technical field.
Background technique
It mainly include physical and chemical composition analysis, Detecting Pesticide during to fresh-cut fruit and vegetable quality and safety detection
With the projects such as microorganism detection.In to fresh-cut fruit and vegetable during the pathogenic microorganism examination, fruits and vegetables is clapped by homogenizer
A large amount of fruits and vegetables residue and small fruit-vegetable granules can be generated after beating, can be the detections such as the counting of subsequent microbe colony, PCR detection
Technology brings different degrees of interference, influences the accuracy of result.
Existing filtering homogenizing bag can only filter bulky grain fruits and vegetables residue, and molecule is still difficult to be removed, and without collection
The processing of pathogenic microorganism.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of Double-filtering device, which can solve fresh-cut fruit and vegetable
There is fruits and vegetables residue and molecule in middle microorganism-collecting.
The present invention solves the above problems a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable of offer, should
Device includes: fruits and vegetables homogeneous collection, large aperture filter membrane, bacterium solution collector, small-bore filter membrane and filtrate displacer.Its
Middle fruits and vegetables homogeneous collection is cylindric hollow structure, and the diameter of two bottom surfaces is greater than the diameter of cylinder, and two bottom edges are prominent
For cylindrical sides, area is removed in two bottom center positions and the circle of column diameter same size forms hollow cylinder, two bottoms
Surrounding protrudes from the structure of side, and is set to top in ground according to central axis, and fruits and vegetables homogeneous collection bottom end is set
Have bacterium solution collector, bacterium solution collector is identical with fruits and vegetables homogeneous collection shape, size, fruits and vegetables homogeneous collection with
Large aperture filter membrane, the area of large aperture filter membrane and the bottom surface of fruits and vegetables homogeneous collection are equipped between bacterium solution collector top
Area is identical, and bacterium solution collector bottom end is equipped with filtrate displacer, and aperture is equipped between bacterium solution collector bottom end and filtrate displacer
The area of diameter filter membrane, small-bore filter membrane is identical as bacterium solution collector bottom end area, filtrate displacer upper end and fruits and vegetables homogeneous
The bottom shape of collection is identical, and filtrate displacer sub-body is that diameter is less than the hollow of filtrate displacer basal diameter
Pipe, the hollow pipe lower end are beveling shape.
The material of the fruits and vegetables homogeneous collection, bacterium solution collector and filtrate displacer is glass material, each section
It clamps protrusion by clip to be fixed, fruits and vegetables homogeneous collection, bacterium solution collector, filtrate displacer, large aperture mistake
It is to be detachably connected between filter membrane and small-bore filter membrane.
The large aperture filter membrane is polypropylene or nylon material, and the material of small-bore filter membrane is nylon material.
When large aperture filter membrane is polypropylene screen, pore size filter range is 10 μm -40 μm, and large aperture filter membrane is nylon mistake
When filter membrane, pore diameter range is 15 μm -60 μm；The pore size filter range of small-bore filter membrane is 0.22 μm -0.45 μm.
The present invention can effectively remove the molecule in fruits and vegetables residue by the filter screen in two kinds of apertures, avoid fruit
Vegetable molecule causes the interference of microorganism-collecting；The present apparatus is that glassware has easy cleaning, corrosion-resistant and resistance to oxidation excellent
Point, reuse rate are high, collect the microorganism of different product, and between do not influence；Each component is detachable simultaneously, is convenient for
Cleaning and sampling, simplify the step of collecting microorganism, improve the quantity for collecting microorganism, and component is detachably also beneficial to strainer
Replacement adapts to the requirement that different microorganisms are collected.
Detailed description of the invention
Fig. 1 is the structure chart of Double-filtering device.
Fig. 2 is the top view of large aperture filter membrane.
Fig. 3 is the top view of small-bore filter membrane.
Such as Fig. 1 fruits and vegetables homogeneous collection, 2. large aperture filter membranes, 3. bacterium solution collectors, 4. small-bore filter membranes, 5.
Filtrate displacer.
Specific embodiment
Below with reference to examples and drawings, detailed description further is made to invention.
Embodiment 1
Fruits and vegetables homogenizing fluid falls in fruits and vegetables homogeneous collection 1, is intercepted using the large aperture filter membrane 2 of 10 μm of polypropylene materials
Residue after fruits and vegetables homogeneous, the filtrate with microorganism reach the small of 0.22 μm of nylon material by bacterium solution collector 3
Bore filter film 4 is discharged by the filtrate of small-bore filter membrane 4 by filtrate displacer 5.
Embodiment 2
Fruits and vegetables homogenizing fluid falls in fruits and vegetables homogeneous collection 1, is intercepted using the large aperture filter membrane 2 of 40 μm of polypropylene materials
Residue after fruits and vegetables homogeneous, the filtrate with microorganism reach the small of 0.45 μm of nylon material by bacterium solution collector 3
Bore filter film 4 is discharged by the filtrate of small-bore filter membrane 4 by filtrate displacer 5.
Embodiment 3
Fruits and vegetables homogenizing fluid falls in fruits and vegetables homogeneous collection 1, intercepts water using the large aperture filter membrane 2 of 15 μm of nylon materials
Residue after fruit and vegetables homogeneous, the filtrate with microorganism reach the aperture of 0.22 μm of nylon material by bacterium solution collector 3
Diameter filter membrane 4 is discharged by the filtrate of small-bore filter membrane 4 by filtrate displacer 5.
Embodiment 4
Fruits and vegetables homogenizing fluid falls in fruits and vegetables homogeneous collection 1, intercepts water using the large aperture filter membrane 2 of 60 μm of nylon materials
Residue after fruit and vegetables homogeneous, the filtrate with microorganism reach the aperture of 0.45 μm of nylon material by bacterium solution collector 3
Diameter filter membrane 4 is discharged by the filtrate of small-bore filter membrane 4 by filtrate displacer 5.
Embodiment 5
By Listeria monocytogenes, Escherichia coli O 157: H7, staphylococcus aureus carry out activation culture, respectively obtain three
The bacterium solution of kind bacterium, sterile working take fresh "Hami" melon sample, 10g sample are weighed, respectively according to 9log cfu/mL artificial contamination three
Kind pathogenic bacteria are put into the sterile homogenizing bag containing 95mL 0.1wt% peptone water after drying up 1h in aseptic operating platform,
It pats and mixes on homogenizer, fruits and vegetables homogenizing fluid is fallen in Double-filtering device, wherein 2 (polypropylene filter of large aperture filter membrane
Film, 10,20,40 μm of pore size) residue after fruits and vegetables homogeneous can be intercepted, the filtrate with microorganism is received by bacterium solution
Storage 3, small-bore filter membrane 4 (nylon filter film, pore size are 0.22 μm and 0.45 μm) collect the micro- life all filtered
Object, filtrate filter through filtered fluid displacer 5.The small-bore filter membrane 4 of attached microbial can be removed, and can carry out subsequent microorganism inspection
It surveys.
Embodiment 6
By Listeria monocytogenes, Escherichia coli O 157: H7, staphylococcus aureus carry out activation culture, respectively obtain three
The bacterium solution of kind bacterium, sterile working take fresh "Hami" melon sample, 10g sample are weighed, respectively according to 9log cfu/mL artificial contamination three
Kind pathogenic bacteria are put into the sterile homogenizing bag containing 90mL 0.1wt% peptone water after drying up 1h in aseptic operating platform,
It pats and mixes on homogenizer, fruits and vegetables homogenizing fluid is fallen in Double-filtering device, large aperture filter membrane 2 (nylon filter film, aperture
15,40,60 μm of size) residue after fruits and vegetables homogeneous can be intercepted, the filtrate with microorganism passes through bacterium solution collector 3,
Small-bore filter membrane 4 (nylon filter film, pore size are 0.22 μm and 0.45 μm) collects the microorganism all filtered, filtrate
It is filtered through filtered fluid displacer.The small-bore filter membrane 4 of attached microbial can be removed, and can carry out subsequent microorganism detection.
Embodiment 7
By Listeria monocytogenes, Escherichia coli O 157: H7, staphylococcus aureus carry out activation culture, respectively obtain three
The bacterium solution of kind bacterium, sterile working take fresh Lettuce samples, by its cutting, 10g sample are weighed, respectively according to 9log cfu/mL people
Work pollutes three kinds of pathogenic bacteria, after drying up 1h in aseptic operating platform, is put into sterile equal containing 90mL 0.1wt% peptone water
It in matter bag, pats and mixes on homogenizer, its homogenizing fluid is fallen in Double-filtering device, 2 (nylon filter of large aperture filter membrane
Film, 15,40,60 μm of pore size) residue after lettuce homogeneous can be intercepted, the filtrate with microorganism passes through bacterium solution collector 3,
Small-bore filter membrane 4 (nylon filter film, pore size are 0.22 μm and 0.45 μm) collects the microorganism all filtered, filtrate
It is filtered through filtered fluid displacer.The small-bore filter membrane 4 of attached microbial can be removed, and can carry out subsequent microorganism detection.
Embodiment 8
By Listeria monocytogenes, Escherichia coli O 157: H7, staphylococcus aureus carry out activation culture, respectively obtain three
The bacterium solution of kind bacterium, sterile working take fresh Lettuce samples, by its cutting, 10g sample are weighed, respectively according to 9log cfu/mL people
Work pollutes three kinds of pathogenic bacteria, after drying up 1h in aseptic operating platform, is put into sterile equal containing 90mL 0.1wt% peptone water
It in matter bag, pats and mixes on homogenizer, lettuce homogenizing fluid is fallen in Double-filtering device, (the nylon mistake of large aperture filter membrane 2
Filter membrane, 15,40,60 μm of pore size) residue after lettuce homogeneous can be intercepted, the filtrate with microorganism passes through bacterium solution collector
3, small-bore filter membrane 4 (nylon filter film, pore size are 0.22 μm and 0.45 μm) collects the microorganism all filtered, filter
Liquid is filtered through filtered fluid displacer.The small-bore filter membrane 4 of attached microbial can be removed, and can carry out subsequent microorganism detection.
As shown in table 1,2,3,4, table 1 is for embodiment 5, embodiment 6, embodiment 7, the microorganism detection result of embodiment 8
The testing result of embodiment 5, table 2 are the testing result of embodiment 6, and table 3 is the testing result of embodiment 7, table 4 is embodiment 8
Testing result.
1 pathogenic microorganism of table is inoculated in the clump count filtered after fresh-cut "Hami" melon through polypropylene screen
2 pathogenic microorganism of table is inoculated in the clump count filtered after fresh-cut "Hami" melon through nylon membrane
3 pathogenic microorganism of table is inoculated in the clump count filtered after fresh-cut water chestnut through polypropylene screen
4 pathogenic microorganism of table is inoculated in the clump count filtered after fresh-cut water chestnut through nylon membrane
The specific embodiment of the invention is explained in detail above, but present invention is not limited to the embodiments described above,
Within the knowledge of those skilled in the art, what can also be made without departing from the inventive concept of the premise is various
Variation each falls within protection scope of the present invention.
Claims (8)
1. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable, which includes: fruits and vegetables homogeneous collection
(1), large aperture filter membrane (2), bacterium solution collector (3), small-bore filter membrane (4) and filtrate displacer (5), which is characterized in that
Fruits and vegetables homogeneous collection (1), large aperture filter membrane (2), bacterium solution collector (3), small-bore filter membrane (4) and filtrate displacer
(5) according to being from top to bottom sequentially arranged.
2. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 1, feature exist
In fruits and vegetables homogeneous collection (1), bacterium solution collector (3) are cylindric hollow structure, and the diameter of two bottom surfaces is greater than cylinder
Diameter, the circle that area and column diameter same size are removed in two bottom center positions forms hollow cylinder, and two bottom surface peripheries are prominent
For the structure of side.
3. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 1 or 2, feature
It is, filtrate displacer (5) upper end is identical as the bottom shape of fruits and vegetables homogeneous collection (1), filtrate displacer (5) lower part master
Body is the hollow pipe that diameter is less than filtrate displacer (5) upper bottom surface diameter, which is beveling shape.
4. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 1, feature exist
In the material of fruits and vegetables homogeneous collection (1), bacterium solution collector (3) and filtrate displacer (5) is glass material.
5. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 1, feature exist
In large aperture filter membrane (2) is one of polypropylene, nylon material, and small-bore filter membrane (4) is nylon material.
6. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 5, feature exist
In large aperture filter membrane (2) is polypropylene screen material, and pore size filter range is 10 μm -40 μm.
7. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 5, feature exist
In large aperture filter membrane (2) is nylon material, and pore size filter range is 15 μm -60 μm.
8. a kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable according to claim 5, feature exist
In the pore size filter range of small-bore filter membrane (4) is 0.22 μm -0.45 μm.
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| CN201910086807.4A CN109762722A (en) | 2019-01-29 | 2019-01-29 | A kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable |
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| CN201910086807.4A CN109762722A (en) | 2019-01-29 | 2019-01-29 | A kind of Double-filtering device for microorganism-collecting in fresh-cut fruit and vegetable |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113637561A (en) * | 2021-07-29 | 2021-11-12 | 中国食品药品检定研究院 | Microbial enrichment device and detection method for multi-impurity large-sample-size liquid |
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Application publication date: 20190517 |