CN215534274U

CN215534274U - Device for recording eye movement, brain electricity and muscle electricity of experimental small animal during sleep

Info

Publication number: CN215534274U
Application number: CN202120913899.1U
Authority: CN
Inventors: 颜彪; 张嘉漪; 檀鑫榕; 孟庆硕; 姜成勇
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2021-04-29
Filing date: 2021-04-29
Publication date: 2022-01-18
Anticipated expiration: 2031-04-29

Abstract

The utility model belongs to the technical field of experimental and scientific research, and particularly relates to a device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleeping. The device is small and portable, has good stability, convenient data export and simple manufacture, has low cost, and can provide a new feasible method for researching the relationship between sleep and eye movement for basic scientific research.

Description

Device for recording eye movement, brain electricity and muscle electricity of experimental small animal during sleep

Technical Field

The utility model belongs to the technical field of experimental scientific research, and particularly relates to a device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleeping. The device is small and portable, data is conveniently exported, the device is simple to manufacture and low in cost, and a novel and feasible method can be provided for basic scientific research and research on the relationship between sleep and eye movement.

Background

According to research, at present, in the scientific research of sleep, data such as brain electricity, myoelectricity and eye movement can be collected in human beings, but most of experimental mice or other experimental animals mainly collect the data of the brain electricity and the myoelectricity, and because the sleep process of the experimental animals can close eyes and the eye movement data is very difficult to collect due to the fact that the eyeballs of the experimental mice are too small, the data of the eye movement of the experimental animals are rarely reported. In the related experiments in the technical field, the eye movement data is recorded mainly on human beings or large primates for recording and data analysis, the eye movement data is recorded mainly by EOG and high-speed cameras for recognizing pupils, only EOG can be used if the eye movement data is recorded in sleep, but the recorded data often has signal interference due to the fact that the position of an EOG recording electrode is not accurate to extraocular muscles, the free movement of an experimental mouse is influenced, and the like. Attention has been paid to exploring the significance of eye movements and the relationship with sleep, etc., in experimental mice.

Based on the current state of the prior art, the inventor of the application intends to provide a device for recording eye movement, brain electricity and muscle electricity of small animals during sleeping. The device can provide a new feasible method for basic scientific research to explore the relationship between sleep and eye movement.

Disclosure of Invention

The utility model aims to provide a device for recording eye movement, brain electricity and myoelectricity of experimental small animals during sleeping based on the current situation of the prior art. The device can provide a new feasible method for basic scientific research to explore the relationship between sleep and eye movement.

The utility model provides a device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleeping. According to the utility model, through designing the circuit board, the interface and the like, electroencephalogram, electromyogram and eye movement signals are concentrated on a small and portable device, and related signals can be all simultaneously input into a computer for recording through a light and thin FPC (flexible printed circuit) cable, so that the device can be used for simultaneously recording eye movement, electroencephalogram and electromyogram data in the processes of free activity, eye-closing sleep and the like of an experimental mouse or other experimental animals, and further researching the significance of eye movement and the relation with sleep of the experimental mouse or other experimental animals. The device is small and portable, data is conveniently exported, the device is simple to manufacture and low in cost, and a new feasible method can be provided for basic scientific research and research on the relationship between sleep and eye movement.

Specifically, the device for recording eye movement, brain electricity and muscle electricity during sleeping of the experimental small animal comprises a magnetic displacement sensor 1, a recording silver wire, a PCB base plate 3, a PCB connecting plate 4 and an FPC connecting interface 5, wherein the sensor and the recording silver wire are simultaneously connected to the front side of the PCB base plate, the PCB connecting plate is vertically welded with the back side of the base plate, and the FPC connecting interface is welded with the PCB connecting plate.

In the utility model, the PCB substrate board can be simultaneously connected with three signal sources of electroencephalogram, myoelectricity and sensors, and signals of eight channels can be recorded simultaneously.

According to the utility model, the welding position of the electroencephalogram and electromyogram signals of the PCB substrate board can reduce the size of the PCB to a greater extent and improve the operability and convenience under the condition that the signals are not influenced mutually.

In the utility model, the PCB connecting plate and the PCB base plate are connected by vertical double-side welding, which is beneficial to saving space and stabilizing the signal output port.

In the utility model, the PCB connecting plate selects the FPC interface as a signal output port, so that the connecting wire is an FPC flat cable, is more portable and is beneficial to free movement of experimental small animals and long-term recording of signals.

More specifically, the device for recording eye movement, brain electricity and myoelectricity of experimental small animals during sleeping mainly comprises a sensor chip 1, brain electricity and myoelectricity silver wires 2, a PCB base plate 3, a PCB connecting plate 4, an FPC interface 5 and five parts,

the two electroencephalogram and electromyogram wires are welded on the PCB base plate, the sensor chip is welded on the base plate, the PCB connecting plate is welded with the back of the base plate, the FPC interface is placed at the correct welding point of the PCB connecting plate, and finally all signals are transmitted to a computer through the FPC cable to be recorded.

The utility model designs a PCB base plate and a PCB connecting plate, wherein the bottom of the base plate is provided with 12 bonding pads, 4 round bonding pads and 8 square bonding pads, the 4 round bonding pads are used for welding two brain electrodes and two myoelectricity electrodes, four of the 8 square bonding pads are a signal anode and a signal cathode of a sensor and a power supply anode and a power supply cathode, and the other four bonding pads are used for fixing the sensor to prevent unstable fixation. The 8 pads for recording signals at the bottom of the base plate are connected with the 8 pads at the back of the base plate through the through holes, and the 8 pads at the back are connected with the PCB connecting plate.

In the embodiment of the utility model, two brain electricity and two muscle electricity silver wires are welded on a PCB base plate, then a layer of epoxy resin AB glue is covered, then a sensor chip is aligned to 3, 4, 5, 6 and 7 of eight bonding pads (shown in figure 3) on the base plate for welding, wherein 3456 is a port with specific signals, 4 and 7 are ports only playing a role in fixing, then eight welding points on the back of the PCB connecting plate and the base plate are welded, then an FPC interface is placed at a correct welding point of the PCB connecting plate, all welding points are welded, and finally all signals are simultaneously transmitted to a computer through an FPC flat cable for recording.

In the embodiment of the utility model, after the manufactured device is tested in vitro, a skull nail with the diameter of 1mm is welded at the tail end of an EEG silver wire, then the device is arranged in the skull of an experimental mouse, the tail end of the EMG silver wire is inserted into the neck muscle of the experimental mouse, the bottom of the whole device, namely a sensor is vertically arranged at the midpoint of a connecting line of two eyeballs of the experimental mouse, the skull nail and the silver wire are covered and exposed by dental cement, and the damaged part of the neck muscle is fixed by smearing biological glue. After the operation is finished, the relevant data can be recorded after the experimental mice recover for one week.

The device combines the electroencephalogram, the myoelectricity and the eye movement record, the anisotropic magneto-resistive sensor can record the eye movement of the experimental small animal in the sleeping process, a recording method is provided for recording the eye movement, the electroencephalogram and the myoelectricity of the experimental small animal in the sleeping process, the sleep and the eye movement basic research can be better combined, and a feasible method is provided for researching the significance of the eye movement in the sleeping process.

The utility model has the advantages that the connecting wire is light, the sensor only needs to be fixed by being attached to the brain, the daily behavior of the experimental small animal is basically not influenced, and the comparison experiment proves that the eye movement signal matching degree recorded by the sensor of the device and the high-speed camera of 50 frames per second is obvious and good, and the device can be operated to record for a long time of at least two months after being implanted at the top of the experimental small animal.

The device is small, exquisite, light, convenient to derive data, good in stability, firm, low in signal noise, high in space-time resolution ratio, simple to manufacture and low in cost, and can provide a new feasible method for basic scientific research on the relationship between sleep and eye movement. Has obvious practical value in the field of sleep and eye movement research.

Drawings

Fig. 1 is a schematic view of an apparatus, wherein,

fig. 1-1 is an anisotropic magnetoresistive sensor, 2 is a pad for brain electricity and muscle electricity, 3 is an independently designed PCB base board for connecting the brain electricity, muscle electricity and eye movement sensors, 4 is an independently designed PCB connecting board for connecting 3 and an FPC interface, 5 is an eight-channel FPC interface, and 6 is an FPC flat cable.

Fig. 2 is a layout view of a PCB substrate board of the present device, wherein,

the left side 1 of the diagram of fig. 2 is a bonding pad for myoelectricity, 2 is a bonding pad for electroencephalogram, silver wires can be welded on the four bonding pads for recording EEG and EMG, 3 and 6 are signal output bonding pads, 4 is a grounding wire bonding pad, 5 is an anode voltage bonding pad, 7 is a fixed bonding pad without actual signal output, 8 is the back side of the perforated connection of the four bonding pads of the sensor, and 9 is the back side of the perforated connection of the bonding pad for electroencephalogram and myoelectricity;

the right side of the figure 2 is a design drawing of a PCB connecting plate of the FPC interface, 1 is the FPC interface, 2 is a fixed welding disc on the FPC interface, 3 is a PCB main body, 4 is a welding disc interface of electroencephalogram and electromyogram signals and eye movement signals, and 5 is a welding disc connected with a base plate.

Fig. 3 is a pictorial view of the apparatus, wherein,

the magnet, the sensor, the PCB base plate, the PCB connecting plate and the FPC interface real object image are arranged from left to right in sequence.

Fig. 4 is a schematic diagram of a mouse recording brain electrical myoelectrical and eye movement signals, wherein,

the middle upper part is a sensor power supply device, the upper right graph is an amplifier and can be used for amplifying signals, the computer screen at the lower right corner can be directly recorded by Spike2 software, EEG is an example of electroencephalogram recording, EMG is an example of electromyogram recording, and sensor is an example of eye movement signal recording.

Figure 5 is an example view of the implantation of the device into an experimental mouse and a complete physical view of the device.

Detailed Description

Example 1

As shown in fig. 1, the device mainly comprises a sensor chip 1, brain-electrical myoelectric silver wires 2, a PCB base plate 3, a PCB connecting plate 4, an FPC interface 5, and five parts, wherein two brain-electrical myoelectric silver wires are welded to 1 and 2 of the PCB base plate (as shown in fig. 3), then covered with a layer of epoxy AB glue, then the sensor chip is aligned to eight pads (as shown in fig. 3) of the base plate, 3, 4, 5, 6, 7 of the third figure, and welding is performed, wherein 3456 is a port with specific signals, 4 and 7 are ports only used for fixing, then the PCB connecting plate is welded with eight welding points on the back of the base plate, 5 of the fourth figure (as shown in fig. 3) is a welding point of the PCB connecting plate and the base plate, then the FPC interface is placed at the correct welding point of the PCB connecting plate, all welding points are welded, and finally, all signals are transmitted to a computer for recording through the FPC cable.

Example 2

After the prepared device is tested in vitro, a skull nail with the diameter of 1mm is welded at the tail end of an EEG silver wire, then the EEG silver wire is screwed into the skull of an experimental mouse, the tail end of an EMG silver wire is inserted into the neck muscle of the experimental mouse, then the bottom of the whole device, namely a sensor is vertically placed at the midpoint of a connecting line of two eyeballs of the experimental mouse, dental cement is used for covering and exposing the skull nail and the silver wire, and the damaged part of the neck muscle of the experimental mouse is coated with biological glue for fixation. After the operation is finished, the data can be recorded when the experimental mouse recovers for one week.

Claims

1. The device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleeping is characterized by comprising a magnetic displacement sensor, a recording silver wire, a PCB (printed circuit board) base plate, a PCB connecting plate and an FPC (flexible printed circuit) connecting interface, wherein the sensor and the recording silver wire are simultaneously connected to the front side of the PCB base plate, the PCB connecting plate is vertically welded to the back side of the base plate, and the FPC connecting interface is welded to the PCB connecting plate.

2. The device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleep according to claim 1, wherein 12 bonding pads are arranged at the bottom of the PCB base plate, 4 circular bonding pads and 8 square bonding pads are arranged at the bottom of the PCB base plate, the 4 circular bonding pads are used for welding two brain electrodes and two muscle electrodes, four of the 8 square bonding pads are signal positive and negative electrodes and power supply positive and negative electrodes of the sensor, and the other four bonding pads are used for fixing the sensor.

3. The device for recording eye movement, brain electricity and muscle electricity during the sleeping period of the experimental small animal as claimed in claim 2, wherein the 8 pads for recording signals at the bottom of the base plate are connected with the 8 pads at the back of the base plate through the through holes, and the 8 pads at the back are connected with the PC B connecting plate.

4. The device for recording eye movement, brain electricity and myoelectricity of experimental small animals during sleeping according to claim 1, wherein the PCB base plate is simultaneously connected with three signal sources of brain electricity, myoelectricity and sensors, and signals of eight channels can be recorded simultaneously.

5. The device for recording eye movement, brain electricity and muscle electricity of experimental small animals during sleeping according to claim 1, wherein the PCB connecting plate and the PCB base plate are connected by vertical double-sided welding, so that space saving and signal output port stability are facilitated.

6. The device for recording eye movement, brain electricity and muscle electricity of the experimental small animal during sleeping according to claim 1, wherein the PCB connecting board selects an FPC interface as a signal output port, so that the connecting line is an FPC (flexible printed circuit) cable, and free movement of the experimental small animal and long-term recording of signals are facilitated.