CN215422300U

CN215422300U - A zooplankton culture apparatus for in water

Info

Publication number: CN215422300U
Application number: CN202120867396.5U
Authority: CN
Inventors: 张亚辉; 杜士林; 张瑜; 魏占亮; 肖欣欣; 黄子晏
Original assignee: Chinese Research Academy of Environmental Sciences
Current assignee: Chinese Research Academy of Environmental Sciences
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2022-01-07
Anticipated expiration: 2031-04-26

Abstract

The utility model provides a zooplankton culture device for a water body, which comprises: a plurality of culture tubes, wherein the upper tube wall and the top surface of each culture tube are provided with a mesh structure; the tray structure is provided with a plurality of culture tube grooves, the culture tube grooves are symmetrically arranged relative to the central axis of the tray structure, and the culture tubes are respectively placed in the culture tube grooves; a central rod is arranged on the upper surface of the tray structure and positioned at the central axis, and the top end of the central rod is connected with a rope; and the tray structure is connected with the positioning device. The zooplankton culture device can be directly applied to a water body, is simple to operate and is convenient for frequent counting operation of zooplanktons.

Description

A zooplankton culture apparatus for in water

Technical Field

The utility model belongs to the field of zooplankton culture experimental devices, and particularly relates to a zooplankton culture device used in a water body.

Background

With the development of industrial economy and the influence of human activities, the problem of water pollution is also increasing. The polluted water body usually contains a large amount of pollutants such as organic matters, nitrogen and phosphorus, inorganic salts and the like. When a certain contaminant is present at a high concentration, it is generally toxic to aquatic organisms over a period of time, resulting in poisoning and death of the aquatic organisms, and therefore, biotoxicity tests are commonly used in the art as test methods for assessing the toxicity of the contaminant.

In order to study the biotoxicity effect caused by the deterioration of water quality. One skilled in the art will typically test aquatic animals as subjects to evaluate the biological toxicity of the water body. Wherein, the zooplankton used for the experiment is represented by Daphnia magna which has the advantages of wide distribution range, easy culture and high sensitivity to various toxic substances. Under laboratory conditions, culture solutions with different target substance concentrations are usually prepared, the daphnia magna is cultured, and the biotoxicity is evaluated according to the death rate of the daphnia magna at different times. For example, chinese patent document CN111830223A discloses a method for evaluating uranium pollution level by using daphnia magna, which specifically comprises: firstly, cultivating the Daphnia magna, and selecting the Daphnia magna which is born for 6-48 hours and is the offspring of the same Daphnia magna as an experimental object; then, the survival conditions of the daphnia magna in the uranium solution are checked and data are recorded through tests of an experimental group and a control group respectively; and finally, processing and analyzing the data to obtain the relation between the death rate of the daphnia magna and the uranium concentration in the water body solution.

The above prior art method for assessing the toxicity level of a contaminant using daphnia magna, through laboratory tests, can obtain the relationship between a single contaminant and the mortality of daphnia magna. However, for the water body with a pollution event, the biological toxicity level of the natural water body can be reflected more by the biological culture test of the water body site. However, up to now, the prior art does not provide a water body test device for culturing zooplankton. Moreover, the life cycle of zooplankton such as daphnia magna is short, and the examination and counting operations are frequently required to be performed in a short time, which undoubtedly brings difficulty to the operation under the natural water body condition.

Disclosure of Invention

Therefore, the technical problem to be solved by the utility model is that a water body test device for culturing zooplankton is lacked in the prior art, and a zooplankton culture device which is applied to a water body, aims at zooplankton and is simple and convenient to operate is further provided.

The technical scheme for solving the technical problems and sampling in the utility model is as follows:

a zooplankton culture apparatus for in a body of water, comprising: a plurality of culture tubes, wherein meshes are arranged on the upper tube wall and the top surface of each culture tube; the tray structure is provided with a plurality of culture tube grooves, the culture tube grooves are symmetrically arranged relative to the central axis of the tray structure, and the culture tubes are respectively placed in the culture tube grooves; a central rod is arranged on the upper surface of the tray structure and positioned at the central axis, and the top end of the central rod is connected with a rope; and the tray structure is connected with the positioning device.

The culture tube groove comprises a metal ring arranged on the upper surface of the tray structure, the axis of the metal ring is arranged along the vertical direction, and the metal ring is fixedly connected with the upper surface of the tray structure through a vertical rod.

The top surface of the culture tube is connected with the tube wall of the culture tube through threads.

The pore diameter of the mesh is 450-500 mu m.

A water quality index monitoring probe is mounted on the tray structure.

The culture tube includes: the glass tube body is of a tubular structure with an opening at the top end; the metal mesh cylinder is provided with openings at two ends and is fixed at the opening at the top end of the glass cylinder body; the top cover is a circular metal mesh plate and is installed at the opening at the top end of the metal mesh cylinder through threads.

The positioning device is a positioning rod.

The central rod of the tray structure is a hollow rod body, and a channel in the central rod penetrates through the top surface of the central rod and the bottom surface of the tray structure; the positioning rod is provided with an installation plate, and the upper plate surface of the installation plate is perpendicular to the axis of the positioning rod; the center rod is sleeved on the positioning rod, and the bottom surface of the tray structure is connected with the upper plate surface of the mounting plate.

Still include the location rope, the one end of location rope with the top of locating lever is connected, and the other end extends to the water top.

The zooplankton culture device for the water body has the advantages that:

the zooplankton culture device for the water body is provided with culture tubes, wherein the upper tube wall and the top surface of each culture tube are provided with mesh structures; the mesh structure can prevent zooplankton from escaping under the condition of ensuring that the water sample of the test device is consistent with the water sample of the water body, and can prevent the zooplankton from being preyed by the organisms in the water body. The tray structure is also provided with a plurality of culture tube grooves, the culture tube grooves are symmetrically arranged relative to the central axis of the tray structure, the culture tubes are respectively placed in the culture tube grooves, a central rod is arranged on the upper surface of the tray structure and positioned on the central axis, and the top end of the central rod is connected with a rope; the lifting operation of the tray structure can be realized by pulling the central rod through the rope.

The zooplankton culture apparatus is also provided with a positioning device, and the tray structure is connected with the positioning device to realize the positioning of the tray structure in the water body. When needs count zooplankton, only need carry and draw the tray structure for the culture tube exposes the surface of water, and the water loss in mesh structure part's the body this moment, but the water in the airtight body of lower part can remain, does not influence zooplankton's life activity, and the back is counted to zooplankton's survival quantity in the body, places again in the water, can continue the biotoxicity test.

According to the zooplankton culture device for the water body, the culture tube groove preferably comprises a metal ring arranged on the upper surface of the tray structure, the axis of the metal ring is arranged in the vertical direction, and the metal ring is fixedly connected with the upper surface of the tray structure through a vertical rod. In addition, still preferred setting the culture tube is the glass material, but this kind of setting mode hoisting device's light transmissivity avoids the cell body to cause the shelter from to the transparent tube body.

According to the zooplankton culture device for the water body, the top surface of the culture tube is preferably connected with the tube wall of the culture tube through threads. So that the top surface can be rotated to open when the survival number is counted each time, and dead zooplankton can be fished out in time.

In addition, the positioning device is limited to be a positioning rod, the center rod of the tray structure is a hollow rod body, and a channel in the center rod penetrates through the top surface of the center rod and the bottom surface of the tray structure; the positioning rod is provided with an installation plate, and the upper plate surface of the installation plate is perpendicular to the axis of the positioning rod; the center rod is sleeved on the positioning rod, and the bottom surface of the tray structure is in contact with the upper plate surface of the mounting plate. The advantage of this kind of mode of setting up can simplify the operation process when the tray structure is put back the water body, only needs insert the locating lever with well core rod on, can accomplish and place. For better location and operation, this application has increased the location rope, the one end of location rope with the top of locating lever is connected, and the other end extends to the water top, introduces the passageway in the well core rod with the upper end from the bottom up of location rope when placing earlier, can play the effect of guide location, simple and convenient experimental operation to the tray structure.

In order to make the technical scheme of the zooplankton culture device for water bodies of the present invention more clearly understood, the present invention is further described with reference to the following specific drawings and specific examples.

Drawings

Fig. 1 is a schematic structural diagram of a zooplankton culture device for use in a water body according to the utility model;

FIG. 2 is a sectional view showing a structure of a culture tube and a tray of the zooplankton culturing apparatus according to the present invention;

wherein the reference numerals are:

1-culture tube; 2-a tray structure; 3-culture tube groove; 31-a metal ring; 32-vertical bar; 4-a central rod; 41-channel; 42-a connecting ring; 5-positioning the rod; 51-a hoisting ring; 6, mounting a plate; 7-probe.

Detailed Description

The embodiment provides a zooplankton culture device for water, in particular to a zooplankton culture device for culturing daphnia magna. As shown in fig. 1 and 2, the zooplankton culturing apparatus includes: the number of the culture tubes 1 is 4 in the present embodiment, and a mesh structure is provided on the upper tube wall and the top surface of each of the culture tubes 1. In a preferred embodiment, the culture tube 1 further comprises a transparent glass tube body, wherein the glass tube body is of a round tube-shaped tubular structure with an open top end; a metal mesh cylinder with openings at two ends is arranged above the glass tube body, and the bottom end of the metal mesh cylinder is fixed at the opening at the top end of the glass tube body. The top cover is arranged at the top opening of the metal mesh cylinder and is a circular metal mesh plate, internal threads are arranged at the top opening of the metal mesh cylinder, external threads are arranged at the edge of the top cover, and the top cover is arranged at the top opening of the metal mesh cylinder through threads. The aperture of the mesh structure on the metal mesh cylinder and the top cover is 450-500 mu m, and the height ratio of the metal mesh cylinder to the glass tube body in the vertical direction is 1: (2-3).

The zooplankton culture device is further provided with a

tray structure

2, 4 culture tube grooves 3 are correspondingly arranged on the tray structure 2, the 4 culture tube grooves 3 are symmetrically arranged relative to the central axis of the tray structure 2, and the plurality of culture tubes 1 are respectively placed in the plurality of culture tube grooves 3; as a preferable embodiment, the culture tube trough 3 in the embodiment comprises a metal ring 31 installed on the upper surface of the tray structure 2, the axis of the metal ring 31 is arranged along the vertical direction, the metal ring 31 is fixedly connected with the upper surface of the tray structure 2 through a vertical rod 32, the cross-sectional diameters of the metal ring 31 and the vertical rod 32 are 3-4mm, and the metal ring and the vertical rod 32 are both supported by thin metal rods, so that the shading effect of the trough body is reduced, and the illumination intensity in the culture tube 1 is consistent with the water body. The upper surface of the tray structure 2 is provided with a central rod 4 at the central axis, the top end of the central rod 4 is provided with two connecting rings 42, and the two connecting rings 42 are connected to a rope through a branch rope respectively for lifting operation of the tray structure 2.

In order to achieve the positioning of the tray structure 2 in the water body, the cultivation device is further provided with a positioning device, with which the tray structure 2 is connected. The positioning device specifically adopts a positioning rod 5, the positioning rod 5 can be directly inserted into the bottom of the water body, or a counterweight platform is arranged at the bottom end of the positioning rod 5, or the positioning rod 5 is fixedly inserted into a fixing pile to realize the fixation in the water body. The top end of the positioning rod 5 is provided with a hanging ring 51 for connecting a positioning rope, one end of the positioning rope is connected with the top end of the positioning rod 5, and the other end of the positioning rope extends to the upper part of the water body.

In this embodiment, the central rod 4 of the tray structure 2 is a hollow rod, and the channel 41 in the central rod 4 is arranged to penetrate through the top surface of the central rod 4 and the bottom surface of the tray structure 2; an installation plate 6 is arranged on the positioning rod 5, and the upper plate surface of the installation plate 6 is perpendicular to the axis of the positioning rod 5; the central rod 4 is sleeved on the positioning rod 5, and the inner diameter of the channel 41 is 1.5-2cm larger than the outer diameter of the positioning rod 5, so that the taking out and placing operations are facilitated. The bottom surface of the tray structure 2 is connected with the upper plate surface of the mounting plate 6.

In order to realize the monitoring of the water quality index, the water quality index monitoring device is further arranged in the embodiment, and a probe 7 of the water quality index monitoring device is arranged on the tray structure 2 and used for detecting various indexes of dissolved oxygen, pH value, inorganic salt, organic matters and the like of the water body, so that data reference is provided for a biotoxicity experiment.

When the zooplankton culture device for the water body in the embodiment is used for a test, the experimental subject is the daphnia magna, and the specific operation method comprises the following steps:

during the experiment, a water sample is placed in the culture tube 1, and cultured Daphnia magna is put in, wherein the Daphnia magna used for the experiment is the Daphnia magna which is born for 6-48 h and is the descendant of the same Daphnia magna. Then place culture tube 1 in on tray structure 2 to with well core rod 4 suit on locating lever 5, place tray structure 2 in on mounting panel 6 on locating lever 5, thereby in arranging the water in, culture tube 1 inner space passes through mesh structure and water intercommunication. Set for count time point, when reaching the time point and need count zooplankton, only need carry and draw tray structure 2 for culture tube 1 exposes to the water surface, and the water in the body of the mesh structure part of each culture tube 1 can run off this moment, but the water in the airtight body of lower part then can remain, does not influence zooplankton's life activity. After the living quantity of zooplankton in the body is counted up, can open the top cap and take out the flea that has died, place the tray base in the water again after accomplishing the count, continue the biotoxicity test, wait for next count time point to take out the observation again. In the process of taking out and putting back the tray structure 2, the positioning rope plays a guiding role, so that the central rod 4 can be sleeved on the positioning rope to catch up.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the utility model.

Claims

1. A zooplankton culture apparatus for in water, its characterized in that includes:

a plurality of culture tubes, wherein meshes are arranged on the upper tube wall and the top surface of each culture tube;

the tray structure is provided with a plurality of culture tube grooves, the culture tube grooves are symmetrically arranged relative to the central axis of the tray structure, and the culture tubes are respectively placed in the culture tube grooves; a central rod is arranged on the upper surface of the tray structure and positioned at the central axis, and the top end of the central rod is connected with a rope;

and the tray structure is connected with the positioning device.

2. The zooplankton culture device of claim 1, wherein the cultivation pipe trough comprises a metal ring mounted on the upper surface of the tray structure, the axis of the metal ring is arranged in a vertical direction, and the metal ring is fixedly connected with the upper surface of the tray structure through a vertical rod.

3. The zooplankton culture device of claim 1, wherein the top surface of the culture tube is in threaded connection with the wall of the culture tube.

4. The zooplankton culture device of claim 3, wherein the aperture of the mesh is 450-500 μm.

5. The zooplankton cultivation device of claim 4, wherein a water quality indicator monitoring probe is mounted on the tray structure.

6. The zooplankton cultivation device of claim 5, wherein the cultivation tube comprises: the glass tube body is of a tubular structure with an opening at the top end; the metal mesh cylinder is provided with openings at two ends and is fixed at the opening at the top end of the glass cylinder body; the top cover is a circular metal mesh plate and is installed at the opening at the top end of the metal mesh cylinder through threads.

7. The zooplankton cultivation device of claim 6, wherein the positioning device is a positioning rod.

8. The zooplankton cultivation device of claim 7, wherein the center pole of the tray structure is a hollow pole body, and the channel in the center pole is disposed through the top surface of the center pole and the bottom surface of the tray structure; the positioning rod is provided with an installation plate, and the upper plate surface of the installation plate is perpendicular to the axis of the positioning rod; the center rod is sleeved on the positioning rod, and the bottom surface of the tray structure is connected with the upper plate surface of the mounting plate.

9. The zooplankton culture device of claim 8, further comprising a positioning rope, wherein one end of the positioning rope is connected to the top end of the positioning rod, and the other end of the positioning rope extends to the upper part of the water body.