CN220712522U - Test device suitable for survey honeybee ability of crawling - Google Patents
Test device suitable for survey honeybee ability of crawling Download PDFInfo
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- CN220712522U CN220712522U CN202321605369.6U CN202321605369U CN220712522U CN 220712522 U CN220712522 U CN 220712522U CN 202321605369 U CN202321605369 U CN 202321605369U CN 220712522 U CN220712522 U CN 220712522U
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- bees
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- 238000012360 testing method Methods 0.000 title claims abstract description 86
- 230000009193 crawling Effects 0.000 title claims abstract description 35
- 241000256844 Apis mellifera Species 0.000 title claims description 25
- 241000257303 Hymenoptera Species 0.000 claims abstract description 67
- 239000011521 glass Substances 0.000 claims abstract description 18
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 10
- 239000010935 stainless steel Substances 0.000 claims abstract description 10
- 231100000331 toxic Toxicity 0.000 abstract description 12
- 230000002588 toxic effect Effects 0.000 abstract description 12
- 230000006399 behavior Effects 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000002159 abnormal effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 description 10
- 241000256837 Apidae Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 208000005374 Poisoning Diseases 0.000 description 3
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 210000003323 beak Anatomy 0.000 description 2
- 231100000739 chronic poisoning Toxicity 0.000 description 2
- 231100000160 chronic toxicity Toxicity 0.000 description 2
- 230000007665 chronic toxicity Effects 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 241000241413 Propolis Species 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 235000013871 bee wax Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 231100001256 chronic oral toxicity Toxicity 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002319 phototactic effect Effects 0.000 description 1
- 229940069949 propolis Drugs 0.000 description 1
- 229940109850 royal jelly Drugs 0.000 description 1
- 230000010019 sublethal effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses a test device suitable for measuring the crawling ability of bees, which comprises a first test box, wherein the top of the first test box is provided with first transparent organic glass, the periphery of the first test box is provided with a first stainless steel net, and the bottom of the first test box is provided with first black organic glass; the second test box, be provided with the black organic glass of second all around of second test box, the bottom is provided with the second stainless steel mesh. The device determines whether the crawling ability of bees is abnormal or not by detecting the proportion of the number of bees passing through the crawling device to the number of surviving bees under the guidance of the light source within 2 minutes and multiplying the crawling passing rate by the group of the bee survival rates, and the test steps can directly and objectively reflect the physiological and ecological behaviors, especially the exercise ability of surviving bees, so that the device can be used for intuitively reflecting the influence of toxic and harmful matters in the environment on the health condition of the bees through some test designs, and has the advantages of convenience, simplicity and strong operability.
Description
Technical Field
The utility model belongs to the technical field of honeybee raising equipment, and particularly relates to a test device suitable for measuring crawling ability of honeybees.
Background
Bees are useful insects with important economic value, and can provide a large amount of bee products for human beings, such as honey, pollen, royal jelly, propolis, beewax and the like. Furthermore, the biggest contribution of bees to humans is that they are an important pollinating insect by which thousands of plants can pollinate throughout the world. The wide distribution, the hair of the whole body, the easy feeding, the strong mobility and the like of bees determine that the bees are very sensitive to the environment, and are often called as environmental pollution biological indicators.
At present, the research on the toxic influence of environmental toxic and harmful substances on bees at home and abroad only stays in the aspect of acute toxicity, and the research on the aspect of chronic toxicity is slow to develop. In particular, the sub-lethal effect, especially the long-term effect, of pesticides on bees has been increasingly emphasized.
For chronic poisoning caused by long-term low-concentration exposure to environmental toxic and harmful substances, in addition to the death condition of bees, attention needs to be paid to the influence of the chronic poisoning on the physiological and ecological behaviors of bees, such as whether various abilities of foraging, flying, sense of direction and the like are disturbed. The existing methods for objectively detecting the bee sublethal reaction in the laboratory comprise the detection of the bee beak extension reaction, the video monitoring movement capability and the like, but the methods are complex in operation, time-consuming and labor-consuming, and are not practical especially for toxicity detection tests.
The crawling ability of the bees is easier to operate and more intuitively observe the change of behaviors than the beak extension reaction, however, the research on the exercise ability of the bees is less. The crawling ability is used for counting the percentage of the number of bees which climb a certain distance from bottom to top to the total number of the tested bees in a certain time. Determining the specified time and crawling length refers to a crawling ability detection experiment of the drosophila, a device for detecting the crawling ability of bees is manufactured, and the time and the height suitable for the crawling ability test of bees are set. After the glass wall is tapped, the bees can not firmly attach the glass wall due to the fact that the friction coefficient is just enough to creep, and fall to the bottom of the box, and the device can meet the detection requirement of the follow-up crawling capability.
The device is designed primarily by utilizing the phototactic and geotactic characteristics of bees to attract the bees to climb upwards to determine the poisoning condition of the bees. The influence condition of environmental toxic and harmful substances on the crawling behavior capability of bees is researched, the purpose is to more objectively know the toxic effect of bees after the bees are contacted with the environmental toxic and harmful substances for a long time at low concentration, and a reference basis is provided for the chronic oral toxicity test standard of the environmental toxic and harmful substances on the bees.
Disclosure of Invention
In order to solve the problems, the utility model provides a test device suitable for measuring the crawling ability of bees.
The technical scheme provided by the utility model is that the test device suitable for measuring the crawling ability of bees comprises a first test box, wherein the top of the first test box is provided with first transparent organic glass, the periphery of the first test box is provided with a first stainless steel net, and the bottom of the first test box is provided with first black organic glass; the second test box, be provided with the black organic glass of second all around of second test box, the bottom of second test box is provided with the second stainless steel mesh.
Further, the top of first test chamber is provided with the light, first bee window that draws has been seted up to the bottom of first test chamber, first sliding cover plate is configured with to first bee window, first bee window is circular.
Further, a second bee-leading window is formed in the bottom of the second test box, a second sliding cover plate is arranged on the second bee-leading window, and the second bee-leading window is circular.
Further, the two sides of the bottom of the second test chamber are provided with supporting blocks.
Further, a light source for emitting light is arranged at the top of the first test box.
Further, a feeder hole is formed in the right side of the bottom of the second test chamber.
The specific operation steps are as follows: 1. collecting healthy worker bees which just emerge from a room for 3-4 days, placing the healthy worker bees into the device, placing a disposable trough in a second test box, feeding sugar water containing sub-lethal dose of toxic and harmful substances according to the growth habit of bees, taking 50% sugar water without toxic and harmful substances as a control, and repeating each dose for 3 times. The new trough needs to be replaced every day, and sugar water with corresponding dosage is regularly supplemented, so that the honey bee can meet daily food requirements of honey bees and can take food at will, and the excrement of the honey bees and dead honey bees are cleaned in time. Continuous feeding for 10d, observing poisoning symptom and death condition of bees every day, and measuring crawling ability of bees at the end of the test.
3. After 10d of treatment, a crawling ability test was performed, which was performed before feeding the new day of medication.
4. The test is carried out in a darkroom, a light source is turned on, an organic glass wall outside a second test box below the light source is knocked off, all bees are knocked off to the bottom of the second test box, meanwhile, a first sliding cover plate is turned on, a stopwatch is pressed down, the total number of bees which climb into the first test box from the second test box within 2 minutes is recorded, the crawling capacity of bees at the end of the test is determined by multiplying the crawling passing rate by the survival rate of the bees according to the proportion of the number of bees to the number of the surviving bees, and each batch of bees are tested for 3 times.
Compared with the prior art, the device has the following beneficial effects: the device can directly and objectively reflect the physiological and ecological behaviors, especially the exercise capacity, of the surviving bees by detecting the proportion of the number of bees passing through the crawling device to the number of surviving bees under the guidance of the light source within 2 min and multiplying the crawling passing rate by the group of the survival rate of bees to determine whether the crawling ability of the bees is abnormal at the end of the test, so that the device can be used for intuitively reflecting the influence of harmful substances on the health condition of the bees, such as the evaluation of the chronic toxicity of sublethal dose of pesticide to the bees.
Drawings
In order to more clearly illustrate the technical solutions of the present utility model, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic illustration of a first test chamber and a second test chamber.
Reference numerals: 1. a first test box, 2, a second test box, 3, a first transparent organic glass, 4, a first black organic glass, 5, a second stainless steel net, 6, a first bee-leading window, 601, a first sliding cover plate, 7, a second bee guiding window 701, a second sliding cover plate, 8, a supporting block, 9, a first stainless steel net, 10, a second black organic glass, 11, a light source, 12 and a feeder hole.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1 and 2, a test device suitable for measuring the crawling ability of bees comprises a first test box 1, wherein the top of the first test box 1 is provided with first transparent organic glass 3, the periphery of the first test box 1 is provided with first stainless steel nets 9, and the bottom of the first test box 1 is provided with first black organic glass 4; the second test chamber 2, be provided with the black plexiglass 10 of second around the second test chamber 2, the bottom of second test chamber 2 is provided with second stainless steel mesh 5.
In this embodiment, the top of the first test chamber is provided with an illumination lamp, the bottom of the first test chamber 1 is provided with a first bee guiding window 6, the first bee guiding window 6 is configured with a first sliding cover plate 601, and the first bee guiding window 601 is circular.
In this embodiment, a second bee guiding window 7 is provided at the bottom of the second test chamber 2, the second bee guiding window 7 is configured with a second sliding cover plate 701, and the second bee guiding window 701 is circular.
In this embodiment, the two sides of the bottom of the second test chamber 2 are provided with supporting blocks 8.
In this embodiment, the top of the first test chamber 1 is provided with a light source 11 for emitting light.
In this embodiment, the bottom right side of the second test chamber 2 is provided with a feeder aperture 12.
The specific operation steps are as follows: 1. collecting healthy worker bees which just emerge from a room for 3-4 days, placing the healthy worker bees into the device, placing a disposable trough in a second test box, feeding sugar water containing sub-lethal dose of toxic and harmful substances according to the growth habit of bees, taking 50% sugar water without toxic and harmful substances as a control, and repeating each dose for 3 times. The new trough needs to be replaced every day, and sugar water with corresponding dosage is regularly supplemented, so that the honey bee can meet daily food requirements of honey bees and can take food at will, and the excrement of the honey bees and dead honey bees are cleaned in time. Continuous feeding for 10d, observing poisoning symptom and death condition of bees every day, and measuring crawling ability of bees at the end of the test.
2. After 10d of treatment, a crawling ability test was performed, which was performed before feeding the new day of medication.
3. The test is carried out in a darkroom, a light source is turned on, an organic glass wall at the outer side of a second test box 2 below the light source is knocked down, all bees are knocked down to the bottom of the second test box, meanwhile, a first sliding cover plate is turned on, a stopwatch is pressed down, the total number of bees which climb into the first test box from the second test box within 2 minutes is recorded, the crawling capacity of bees at the end of the test is determined by multiplying the crawling passing rate by the survival rate of the bees according to the proportion of the number of bees to the number of the surviving bees, and each batch of bees is tested for 3 times.
While the utility model has been described with respect to the preferred embodiments, it will be understood that the utility model is not limited thereto, but is capable of modification and variation without departing from the spirit of the utility model, as will be apparent to those skilled in the art.
Claims (6)
1. Test device suitable for survey honeybee ability of crawling, its characterized in that: the device comprises a first test box, wherein the top of the first test box is provided with first transparent organic glass, a first stainless steel net is arranged around the first test box, and the bottom of the first test box is provided with first black organic glass;
the second test box, be provided with the black organic glass of second all around of second test box, the bottom of second test box is provided with the second stainless steel mesh.
2. A test device suitable for determining the crawling ability of bees according to claim 1, characterized in that: the top of first test chamber is provided with the light, first bee window has been seted up to the bottom of first test chamber, first bee window disposes first slip apron, first bee window is circular.
3. A test device suitable for determining the crawling ability of bees according to claim 1, characterized in that: the bottom of the second test box is provided with a second bee guiding window, the second bee guiding window is provided with a second sliding cover plate, and the second bee guiding window is round.
4. A test device suitable for determining the crawling ability of bees according to claim 1, characterized in that: and the two sides of the bottom of the second test chamber are provided with supporting blocks.
5. A test device suitable for determining the crawling ability of bees according to claim 1, characterized in that: the top of the first test box is provided with a luminous light source.
6. A test device suitable for determining the crawling ability of bees according to claim 1, characterized in that: and a feeder hole is formed in the right side of the bottom of the second test chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321605369.6U CN220712522U (en) | 2023-06-21 | 2023-06-21 | Test device suitable for survey honeybee ability of crawling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321605369.6U CN220712522U (en) | 2023-06-21 | 2023-06-21 | Test device suitable for survey honeybee ability of crawling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220712522U true CN220712522U (en) | 2024-04-05 |
Family
ID=90485317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321605369.6U Active CN220712522U (en) | 2023-06-21 | 2023-06-21 | Test device suitable for survey honeybee ability of crawling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220712522U (en) |
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2023
- 2023-06-21 CN CN202321605369.6U patent/CN220712522U/en active Active
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