CN215375438U - Simple potato virus enzyme-linked immunoassay operation panel - Google Patents
Simple potato virus enzyme-linked immunoassay operation panel Download PDFInfo
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- CN215375438U CN215375438U CN202121720135.7U CN202121720135U CN215375438U CN 215375438 U CN215375438 U CN 215375438U CN 202121720135 U CN202121720135 U CN 202121720135U CN 215375438 U CN215375438 U CN 215375438U
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Abstract
The utility model discloses a simple potato virus enzyme-linked immunoassay console, which relates to the technical field of experiment consoles and can provide a qualified experiment environment with low cost for basic laboratories which lack heating conditions and are not fully equipped with equipment, thereby ensuring the accuracy of detection results, and the specific scheme is as follows: including open-top's box body, the box body bottom is equipped with the accuse temperature layer, is equipped with temperature regulating device in the accuse temperature layer, and the box body middle part is vertical to be equipped with the shielding plate, and the shielding plate falls into buffer solution with the box body and places district and enzyme-linked reaction district, and enzyme-linked reaction district open-top corresponds and is equipped with the lid, and enzyme-linked reaction district bottom, lateral wall and the lid that corresponds are light-tight material. The simple potato virus enzyme-linked immunoassay console provided by the utility model meets the requirements of temperature, humidity and light resistance in the enzyme-linked immunoassay environmental index, and ensures the accuracy of the detection result; the simple potato virus enzyme-linked immunoassay operating table device has the advantages of more applicable materials, simple structure and easy processing.
Description
Technical Field
The utility model relates to the technical field of experiment operation tables, in particular to a simple potato virus enzyme-linked immunoassay operation table.
Background
In potato virus enzyme-linked immunosorbent assay (ELISA), the temperature has an influence on the pH value, the optimal working temperature of a buffer solution is 22-25 ℃, the buffer solution is stored at 4 ℃ generally, but the buffer solution is easy to crystallize in the storage process, the buffer solution has poor uniformity under the condition of low room temperature in autumn and winter, the detection quality can be influenced by direct use of the buffer solution, and the buffer solution can meet the working requirement by placing the buffer solution for temperature return or heating for temperature rise generally.
In the incubation process, the temperature range is usually 4 ℃ or 37 ℃, the reaction time is longer when the temperature is lower, and in order to improve the detection speed, the incubation at 37 ℃ is more commonly used, and the step is usually carried out in a specific instrument (constant temperature incubator), so the instrument cost is higher.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problems, the utility model provides a simple potato virus enzyme-linked immunoassay console which can provide a qualified experimental environment with low cost for a basic laboratory lacking in heating condition and incomplete equipment configuration, thereby ensuring the accuracy of a detection result.
The technical purpose of the utility model is realized by the following technical scheme:
the utility model provides a simple and easy potato virus enzyme-linked immunosorbent assay operation panel, includes open-top's box body, and the box body bottom is equipped with the accuse temperature layer, is equipped with temperature regulating device in the accuse temperature layer, and the box body middle part is vertical to be equipped with the shielding plate, and the shielding plate falls into the buffer solution with the box body and places district and enzyme-linked reaction district, and enzyme-linked reaction district open-top corresponds and is equipped with the lid, and enzyme-linked reaction district bottom, lateral wall and the lid that corresponds are light-tight material.
In the above scheme, the bottom of the box body can be provided with a cavity as a temperature control layer, a temperature control device which can be taken and placed can be used, such as carbon fire, and a fixed temperature control device can be used, such as an electric heating wire and the like; then a baffle plate is arranged in the box body and is placed on the temperature control layer, and then the upper space of the temperature control layer is divided into two areas which are respectively used for placing buffer solution and carrying out enzyme-linked reaction.
When the buffer solution is used, firstly, the stored buffer solution is placed in the buffer solution placing area to be heated, after the buffer solution is heated to the appropriate temperature, the buffer solution is diluted, the buffer solution is added to the enzyme-linked plate after the buffer solution is diluted to the working concentration, then the buffer solution is sleeved with a freshness protection bag or covered with a cover to preserve moisture, the buffer solution is placed in the enzyme-linked reaction area, the cover is covered, the whole enzyme-linked reaction area is protected from light in the whole environment, and then the buffer solution is continuously heated to the appropriate temperature for incubation.
Above-mentioned scheme can effectively retreat the heating process of buffer solution and the heating process of enzyme-linked reaction for buffer solution and enzyme-linked reaction process all can reach best operating temperature, and then improve the rate of accuracy of testing result.
Preferably, the box cover is hinged with the top end of the side wall of the box body.
In above-mentioned scheme, lid and box body are connected for independent box body structure for the wholeness of device is better, avoids the loss of lid to cause inconveniently.
Preferably, the shielding plate is made of a heat insulating material.
In above-mentioned preferred scheme, use insulation material, can be fine preserve the temperature, after heating the buffer solution, can avoid losing of heat, and then reduce the required heat in the enzyme-linked reaction process, and then reach energy-conserving purpose.
As a preferred scheme, the temperature control device is a carbon fiber heating wire which is electrically connected with a temperature control regulator.
In above-mentioned preferred scheme, carry out temperature regulation through temperature control regulator to the carbon fiber heater strip, and then the temperature in the effective control box body, and then improve the rate of accuracy of testing result.
Preferably, the temperature controller is fixedly connected to an outer side wall of the case body.
In above-mentioned preferred scheme, suspend for temperature control regulator, fixed connection is on the box body, can make whole device integral type better, portable and place, can also effectively avoid temperature control regulator the condition of damage to appear simultaneously.
As a preferred scheme, temperature sensors are respectively arranged in the buffer solution placing area and the enzyme-linked reaction area, two display screens are respectively arranged on the outer side walls of the buffer solution placing area and the enzyme-linked reaction area, and the two display screens are respectively used for displaying the temperature in the buffer solution placing area and the temperature in the enzyme-linked reaction area.
In above-mentioned preferred scheme, acquire the temperature in the box body through the display screen, and then accurate use temperature control regulator carries out temperature control, and the experimental environment of the heating condition and the enzyme-linked reaction of better acquisition buffer solution, and then effectively improve the accuracy that detects.
In conclusion, the utility model has the following beneficial effects:
(1) the simple potato virus enzyme-linked immunoassay console provided by the utility model meets the requirements of temperature, humidity and light resistance in the enzyme-linked immunoassay environmental index, and ensures the accuracy of the detection result;
(2) the simple potato virus enzyme-linked immunoassay operation table device provided by the utility model has the advantages of more applicable materials, simple structure and easy processing;
(3) the simple potato virus enzyme-linked immunoassay operating table provided by the utility model can replace equipment such as a thermostat and the like, and has the advantages of less power consumption, small occupied area, energy conservation and money saving.
Drawings
FIG. 1 is a schematic structural diagram of a simple potato virus ELISA console according to an embodiment of the present invention;
FIG. 2 is a front view of a simplified potato virus ELISA test console according to an embodiment of the present invention;
reference numerals:
1. a box body; 2. a temperature control layer; 3. a temperature control device; 4. a shielding plate; 5. a buffer placement area; 6. an enzyme-linked reaction zone; 7. a box cover; 8. a temperature control regulator.
Detailed Description
This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. "substantially" means within an acceptable error range, within which a person skilled in the art can solve the technical problem to substantially achieve the technical result.
The terms in upper, lower, left, right and the like in the description and the claims are combined with the drawings to facilitate further explanation, so that the application is more convenient to understand and is not limited to the application.
The present invention will be described in further detail with reference to the accompanying drawings.
The utility model provides a simple and easy potato virus enzyme-linked immunosorbent assay operation panel, includes open-top's box body, and the box body bottom is equipped with the accuse temperature layer, is equipped with temperature regulating device in the accuse temperature layer, and the box body middle part is vertical to be equipped with the shielding plate, and the shielding plate falls into the buffer solution with the box body and places district and enzyme-linked reaction district, and enzyme-linked reaction district open-top corresponds and is equipped with the lid, and enzyme-linked reaction district bottom, lateral wall and the lid that corresponds are light-tight material.
In the above embodiment, the bottom of the box body may have a cavity as a temperature control layer, and may use a temperature control device capable of being taken and placed, such as a carbon fire, or may use a fixed temperature control device, such as an electric heating wire; then a baffle plate is arranged in the box body and is placed on the temperature control layer, and then the upper space of the temperature control layer is divided into two areas which are respectively used for placing buffer solution and carrying out enzyme-linked reaction.
When the buffer solution is used, firstly, the stored buffer solution is placed in the buffer solution placing area to be heated, after the buffer solution is heated to the appropriate temperature, the buffer solution is diluted, the buffer solution is added to the enzyme-linked plate after the buffer solution is diluted to the working concentration, then the buffer solution is sleeved with a freshness protection bag or covered with a cover to preserve moisture, the buffer solution is placed in the enzyme-linked reaction area, the cover is covered, the whole enzyme-linked reaction area is protected from light in the whole environment, and then the buffer solution is continuously heated to the appropriate temperature for incubation.
The embodiment can effectively remove the heating process of the buffer solution and the heating process of the enzyme-linked reaction, so that the buffer solution and the enzyme-linked reaction process can reach the optimal working temperature, and the accuracy of the detection result is improved.
As a preferred embodiment, the box cover is hinged with the top end of the side wall of the box body.
In the above embodiment, the box cover and the box body are connected, so that the integrity of the device is better and inconvenience caused by loss of the box cover is avoided compared with an independent box body structure.
In a preferred embodiment, the shielding is made of a heat insulating material.
In the preferred embodiment, the heat preservation material is used, so that the temperature can be well preserved, and after the buffer solution is heated, the heat loss can be avoided, so that the heat required in the enzyme-linked reaction process is reduced, and the purpose of saving energy is achieved.
As a preferred embodiment, the temperature control device is a carbon fiber heating wire which is electrically connected with a temperature control regulator.
In the preferred embodiment, the temperature of the carbon fiber heating wire is adjusted through the temperature control adjuster, so that the temperature in the box body is effectively controlled, and the accuracy of the detection result is improved.
As a preferred embodiment, the temperature controller is fixedly connected to an outer sidewall of the case.
In the above preferred embodiment, the temperature control regulator is suspended and fixedly connected to the box body, so that the whole device is better integrated, is convenient to carry and place, and can effectively avoid the temperature control regulator from being damaged.
As a preferred embodiment, temperature sensors are respectively arranged in the buffer solution placing area and the enzyme-linked reaction area, two display screens are respectively arranged on the outer side walls of the buffer solution placing area and the enzyme-linked reaction area, and the two display screens are respectively used for displaying the temperature in the buffer solution placing area and the temperature in the enzyme-linked reaction area.
In the preferred embodiment, the temperature in the box body is obtained through the display screen, the temperature controller is used for temperature control accurately, the heating condition of the buffer solution and the experimental environment of the enzyme-linked reaction are obtained better, and the detection accuracy is further improved effectively.
A semi-closed square box, wherein the inner part has two regions, one end is an open buffer solution placing region at the temperature of 22-25 ℃, the other end is an open closed enzyme-linked reaction region at the temperature of 37 ℃, and the middle heat-insulating material is used for isolation.
The square box is made of any heat preservation material, the two bottom areas are respectively controlled in temperature, the bottom temperature is controlled by carbon fiber for heating, a temperature control switch is arranged, and the enzyme-linked reaction area is protected from light in the whole environment.
The working principle is as follows:
the method comprises the following steps: after the buffer solution storage liquid is heated to the working requirement in the buffer solution placing area, taking out the buffer solution storage liquid and immediately diluting the buffer solution storage liquid to the working concentration;
step two: adding the required antibody into the diluted buffer solution, shaking gently, shaking uniformly, adding 100ul/200ul of the diluted buffer solution into the enzyme-linked plate, covering a safety bag or covering a cover to preserve moisture, and putting the enzyme-linked plate into a closed reaction zone to incubate for 2-4h at 37 ℃.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. The utility model provides a simple and easy potato virus enzyme-linked immunosorbent assay operation panel, its characterized in that, including open-top's box body, the box body bottom is equipped with the accuse temperature layer, is equipped with temperature regulating device in the accuse temperature layer, and the box body middle part is vertical to be equipped with the shielding plate, and the shielding plate falls into the buffer solution with the box body and places district and enzyme-linked immunosorbent assay district, and enzyme-linked immunosorbent assay district open-top corresponds and is equipped with the lid, and enzyme-linked immunosorbent assay district bottom, lateral wall and the lid that corresponds are light-tight material.
2. The simple potato virus enzyme-linked immunoassay console of claim 1, wherein the box cover is hinged with the top end of the side wall of the box body.
3. The simple potato virus enzyme-linked immunoassay console of claim 1, wherein the shielding plate is made of a heat insulating material.
4. The simple potato virus enzyme-linked immunoassay console of claim 1, wherein the temperature control device is a carbon fiber heating wire, and the carbon fiber heating wire is electrically connected with a temperature control regulator.
5. The simple potato virus enzyme-linked immunoassay console of claim 4, wherein the temperature control regulator is fixedly connected to the outer side wall of the box body.
6. The simple potato virus enzyme-linked immunoassay console of claim 1, wherein the buffer solution placing area and the enzyme-linked reaction area are respectively provided with a temperature sensor, the outer side walls of the buffer solution placing area and the enzyme-linked reaction area are respectively provided with two display screens, and the two display screens are respectively used for displaying the temperature in the buffer solution placing area and the temperature in the enzyme-linked reaction area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121720135.7U CN215375438U (en) | 2021-07-27 | 2021-07-27 | Simple potato virus enzyme-linked immunoassay operation panel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121720135.7U CN215375438U (en) | 2021-07-27 | 2021-07-27 | Simple potato virus enzyme-linked immunoassay operation panel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215375438U true CN215375438U (en) | 2021-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121720135.7U Active CN215375438U (en) | 2021-07-27 | 2021-07-27 | Simple potato virus enzyme-linked immunoassay operation panel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215375438U (en) |
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2021
- 2021-07-27 CN CN202121720135.7U patent/CN215375438U/en active Active
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