CN215365784U - Citric acid bacterial machine - Google Patents

Citric acid bacterial machine Download PDF

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Publication number
CN215365784U
CN215365784U CN202121542822.4U CN202121542822U CN215365784U CN 215365784 U CN215365784 U CN 215365784U CN 202121542822 U CN202121542822 U CN 202121542822U CN 215365784 U CN215365784 U CN 215365784U
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bin body
stirring
citric acid
culture
bin
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CN202121542822.4U
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Chinese (zh)
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苏安国
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Beijing Meckey Machinery Engineering Co ltd
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Beijing Meckey Machinery Engineering Co ltd
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Abstract

The utility model provides a citric acid bacterial machine, which comprises a culture bin, a culture bin and a control system, wherein the culture bin is formed by hermetically combining an upper bin body and a lower bin body; the stirring device comprises a motor, a speed reducer, a stirring rack and a stirrer which are connected in sequence; the motor, the speed reducer and the stirring frame are all positioned on the outer side of the upper bin body; the stirrer comprises a stirring rod and a stirring cross rod arranged at the end part of the stirring rod; the stirring rod penetrates through the culture bin from the upper bin body; the stirring cross rod is positioned at the lower bin body of the culture bin, and a plurality of stirring claws are arranged at one side of the stirring cross rod at intervals; one side of the upper bin body is provided with a culture medium feeding hole and an inoculation port; one side of the lower bin body is provided with a gas-liquid valve which is connected with a distributor positioned in the bin discharging bin; the distributor is positioned above the stirring cross rod, and a plurality of spray heads are arranged on one side of the distributor at intervals. The citric acid bacteria machine has high industrialization degree and high production efficiency.

Description

Citric acid bacterial machine
Technical Field
The utility model belongs to the field of biological cultivation equipment, and particularly relates to a citric acid bacterial machine.
Background
In citric acid production, the expanded culture of strains is an important link of the fermentation process. In citric acid fermentation enterprises, a large amount of aspergillus niger spores are used for inoculation. At present, the strains used in the industry are basically cultured by using a 1-2L triangular flask. When the volume of the fermentation tank reaches 300-1000 cubic meters, hundreds of triangular flask strains are needed to be used in each fermentation tank. At present, the strain preparation by using a triangular flask is not industrialized, the labor intensity is high, the production efficiency is low, and the strain is easy to contaminate.
Or as patent publication No. CN211946976U discloses a bacterial culture machine, including the device main part, the upper end of device main part is provided with the top cap, the upper end surface of top cap runs through and is provided with the spliced pole, the inboard of spliced pole runs through and is provided with the blow vent, the surface of spliced pole is connected with the aeration pipe, the surface of spliced pole is close to the below of aeration pipe and is provided with the liquid storage tank, the surface of one side of device main part extends there is the protection box, the surface of one side of liquid storage tank extends the fluid-discharge tube, the fluid-discharge tube is located the inboard of protection box, the outside of protection box is stamped sealed apron.
In view of this, a citric acid bacterial machine which has high industrialization degree and high production efficiency is urgently needed to be provided.
SUMMERY OF THE UTILITY MODEL
Therefore, the technical problem to be solved by the utility model is to provide the citric acid bacterial machine with high production efficiency.
The citric acid bacterial machine comprises:
the culture bin is formed by hermetically combining an upper bin body and a lower bin body;
the stirring device comprises a motor, a speed reducer, a stirring rack and a stirrer which are connected in sequence; the motor, the speed reducer and the stirring rack are all positioned on the outer side of the upper bin body; the stirrer comprises a stirring rod and a stirring cross rod arranged at the end part of the stirring rod; the stirring rod penetrates through the culture bin from the upper bin body; the stirring cross rod is positioned at the position of the culture bin on the lower bin body, and a plurality of stirring claws are arranged on one side of the stirring cross rod at intervals;
a culture medium feeding hole and an inoculation port are formed in one side of the upper bin body;
a gas-liquid valve is arranged on one side of the lower bin body and is connected with a distributor positioned in the bin discharging bin; the distributor is located above the stirring cross rod, and a plurality of spray headers are arranged on one side of the distributor at intervals.
Furthermore, a pressure sensor interface is arranged outside the upper bin body.
Furthermore, a temperature and humidity sensor interface is arranged outside the upper bin body.
Furthermore, an exhaust port is arranged outside the upper bin body.
Furthermore, an observation device is arranged outside the upper bin body, and the observation device comprises a sight lamp and a sight glass which are sequentially connected with the upper bin body.
Furthermore, a discharge hole is formed in the center of the bottom of the lower bin body.
Furthermore, a heat insulation layer coated along the outline of the outer edge of the lower bin body is arranged outside the lower bin body.
Furthermore, the bottom of the lower bin body is provided with a supporting leg.
Furthermore, a sealing element is arranged between the upper bin body and the stirrer frame.
Further, the device also comprises a power supply which is connected with the motor.
Compared with the prior art, the technical scheme of the utility model has the following advantages:
according to the utility model, a culture bin is formed between an upper bin body and a lower bin body which are sealed, a basic environment suitable for culturing citric acid bacteria is formed in the culture bin, then culture medium is filled into the culture bin through a culture medium feeding hole, the culture medium in the culture bin is fully stirred through a stirring device, and meanwhile, compressed air, compressed water or steam is added into the culture bin through a gas-liquid valve, so that the environment in the culture bin is regulated in real time, and the industrial production of the citric acid bacteria is realized.
Drawings
FIG. 1 is a schematic structural diagram of a citric acid bacterial machine provided by an embodiment of the utility model;
wherein, 1, supporting legs; 2. a heat-insulating layer; 3. a blender; 4. a distributor; 5. a gas-liquid valve; 6. a culture bin; 7. an upper bin body; 8. a culture medium feed port; 9. a seal member; 10. a mixer frame; 11. a speed reducer; 12. a motor; 13. an exhaust port; 14. inoculating; 15. a pressure sensor interface; 16. a temperature and humidity sensor interface; 17. a sight lamp; 18. a sight glass; 19. a shower head; 20. a lower bin body; 21. and (4) a discharge port.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The citric acid bacterial culturing machine of the present embodiment, as shown in fig. 1, includes a culturing chamber 6 and a stirring device.
The culture bin 6 is formed by hermetically combining an upper bin body 7 and a lower bin body 20. The bottom of the lower bin body 20 is provided with a supporting leg 1, and the present embodiment is fixed on the ground surface through the supporting leg 1.
The stirring device comprises a motor 12, a speed reducer 11, a stirrer 3 frame and a stirrer 3 which are connected in sequence; the motor 12, the speed reducer 11 and the stirrer 3 are all positioned outside the upper bin body 7; the stirrer 3 comprises a stirring rod and a stirring cross rod arranged at the end part of the stirring rod; the stirring rod is arranged in the culture bin 6 which is penetrated by the upper bin body 7; the stirring cross bar is positioned at the position of the culture bin 6 on the lower bin body 20, and a plurality of stirring claws are arranged on one side of the stirring cross bar at intervals. And a sealing element 9 is arranged between the upper bin body 7 and the stirrer 3 to ensure the sealing property.
One side of the upper bin body 7 is provided with a culture medium feed port 8 and an inoculation port 14, the culture medium feed port 8 is used for adding a culture medium into the culture bin 6, and the inoculation port 14 is used for inoculating citric acid bacteria onto the culture medium.
A gas-liquid valve 5 is arranged on one side of the lower bin body 20, and the gas-liquid valve 5 is connected with a distributor 4 positioned in the bin discharging bin; the distributor 4 is positioned above the stirring cross rod, a plurality of spray headers 19 are arranged at intervals on one side of the distributor 4, high-temperature steam, sterile air and sterile water are introduced into the culture bin 6 through the gas-liquid valve 5, and are sprayed on the culture medium through the spray headers 19.
The upper bin body 7 is externally provided with a pressure sensor interface 15 which is used for being connected with a pressure sensor to monitor whether the pressure environment in the culture bin 6 is suitable for the growth of the citric acid bacteria.
And a temperature and humidity sensor interface 16 is arranged outside the upper bin body 7 and is used for being connected with a pressure sensor to monitor whether the temperature and humidity environment in the culture bin 6 is suitable for the growth of the citric acid bacteria.
An exhaust port 13 is arranged outside the upper bin body 7 and used for exhausting the gas in the culture bin 6 so as to balance the air pressure and the temperature in the culture bin 6.
The center of the bottom of the lower bin body 20 is provided with a discharge hole 21, and the citric acid bacteria can be sampled and produced without opening the culture bin 6 by arranging the discharge hole 21.
An observation device is arranged outside the upper bin body 7 and comprises a sight lamp 17 and a sight glass 18 which are sequentially connected with the upper bin body 7 and used for microscopic examination of the samples of the citric acid bacteria to ensure the growth state of the citric acid bacteria.
The outer heat preservation 2 that is equipped with along the outer edge profile cladding of lower storehouse body 20 is equipped with outside lower storehouse body 20, because cultivate the storehouse 6 and make by the metal material, heat conduction rate is very fast, in order to reduce the loss of temperature, sets up heat preservation 2 outside lower storehouse body 20, guarantees that the temperature in cultivating the storehouse 6 is fit for the growth of citric acid bacterium.
The embodiment also comprises a power supply which is connected with the motor 12; the motor 12 is powered by a power supply to realize the normal operation of the stirring device.
The working principle of the citric acid bacterial machine in the embodiment is as follows: firstly, the culture medium is prepared according to the preset proportion, then the culture medium is added into the culture bin 6 from the culture medium feed port 8, the stirring device is opened, the culture medium is uniformly stirred by the stirrer 3, and after the culture medium feeding and stirring are completed, the culture medium feed port 8 is sealed. Then, the gas-liquid valve 5 is opened, high-temperature steam is filled into the culture chamber 6, and the temperature in the culture chamber 6 is raised to a predetermined temperature, thereby completing sterilization of the culture medium. Then, the exhaust port 13 is opened to cool the culture chamber 6. After the temperature is reduced, inoculating citric acid bacteria into the culture bin 6 through the inoculation port 14, monitoring the temperature and the humidity in the culture bin 6 in real time, then opening the stirring device, introducing sterile air into the culture bin 6 through the gas-liquid valve 5, and culturing the citric acid bacteria.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the utility model.

Claims (10)

1. A citric acid bacterial machine, comprising:
the culture bin is formed by hermetically combining an upper bin body and a lower bin body;
the stirring device comprises a motor, a speed reducer, a stirring rack and a stirrer which are connected in sequence; the motor, the speed reducer and the stirring rack are all positioned on the outer side of the upper bin body; the stirrer comprises a stirring rod and a stirring cross rod arranged at the end part of the stirring rod; the stirring rod penetrates through the culture bin from the upper bin body; the stirring cross rod is positioned at the position of the culture bin on the lower bin body, and a plurality of stirring claws are arranged on one side of the stirring cross rod at intervals;
a culture medium feeding hole and an inoculation port are formed in one side of the upper bin body;
a gas-liquid valve is arranged on one side of the lower bin body and is connected with a distributor positioned in the culture bin; the distributor is located above the stirring cross rod, and a plurality of spray headers are arranged on one side of the distributor at intervals.
2. The citric acid bacteria machine of claim 1, wherein the upper bin body is externally provided with a pressure sensor interface.
3. The citric acid bacteria machine of claim 1, wherein a temperature and humidity sensor interface is arranged outside the upper bin body.
4. The citric acid bacteria machine of claim 1, wherein an air vent is arranged outside the upper bin body.
5. The citric acid bacteria machine of claim 1, wherein an observation device is arranged outside the upper bin body, and the observation device comprises a sight lamp and a sight glass which are sequentially connected with the upper bin body.
6. The citric acid bacteria machine of claim 1, wherein a discharge hole is arranged at the center of the bottom of the lower bin body.
7. The citric acid bacteria machine of claim 1, wherein an insulating layer coated along the outline of the outer edge of the lower bin body is arranged outside the lower bin body.
8. The citric acid bacteria machine of claim 1, wherein the bottom of the lower bin body is provided with support legs.
9. The citric acid bacteria machine of claim 1, wherein a sealing member is arranged between the upper bin body and the mixer frame.
10. The citric acid bacteria machine of claim 1, further comprising a power source connected to said motor.
CN202121542822.4U 2021-07-07 2021-07-07 Citric acid bacterial machine Active CN215365784U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121542822.4U CN215365784U (en) 2021-07-07 2021-07-07 Citric acid bacterial machine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121542822.4U CN215365784U (en) 2021-07-07 2021-07-07 Citric acid bacterial machine

Publications (1)

Publication Number Publication Date
CN215365784U true CN215365784U (en) 2021-12-31

Family

ID=79608038

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121542822.4U Active CN215365784U (en) 2021-07-07 2021-07-07 Citric acid bacterial machine

Country Status (1)

Country Link
CN (1) CN215365784U (en)

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