CN215365735U - Separation device for ovarian cancer circulating tumor cell exosomes - Google Patents
Separation device for ovarian cancer circulating tumor cell exosomes Download PDFInfo
- Publication number
- CN215365735U CN215365735U CN202121192962.3U CN202121192962U CN215365735U CN 215365735 U CN215365735 U CN 215365735U CN 202121192962 U CN202121192962 U CN 202121192962U CN 215365735 U CN215365735 U CN 215365735U
- Authority
- CN
- China
- Prior art keywords
- fixed
- circulating tumor
- ovarian cancer
- bottom plate
- motor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of separation devices, and discloses a separation device for ovarian cancer circulating tumor cell exosomes, which comprises a bottom plate, wherein a shaking device is arranged above the bottom plate, a containing device is arranged above the shaking device, stand columns are fixed on the left side and the right side of the upper surface of the bottom plate, a first connecting piece is fixed at one end, far away from the bottom plate, of each stand column, the shaking device comprises a motor, a rotating rod is fixed at the output end of the motor, a rotating disc is fixed at one end, far away from the motor, of each rotating rod, and a sliding column is fixed on the left side of the upper surface of the rotating disc. This separator of ovarian cancer circulating tumor cell exosome through the cooperation that sets up between motor, rotary rod, carousel, slip post, base and the sliding tray, can drive the bracing piece and place the dish and rock in a certain angle slope, consequently can shake the liquid in the test tube evenly to replace the manual work to shake even operation, improved work efficiency.
Description
Technical Field
The utility model relates to the technical field of separation devices, in particular to a separation device for ovarian cancer circulating tumor cell exosomes.
Background
The conventional methods for exosome separation are differential ultracentrifugation and density gradient ultracentrifugation, etc., the density gradient ultracentrifugation requires ultracentrifugation of a sample and a gradient material together, and different components in the sample settle into respective equal density regions, thereby causing inconvenience in extracting exosomes from different precipitates.
The existing separation device has single function, can carry out centrifugal separation only after shaking cell solution evenly by manpower, and has certain limitation when in use.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
Aiming at the defects of the prior art in the background art, the utility model provides a separating device for an ovarian cancer circulating tumor cell exosome, which overcomes the defects that the existing separating device for the ovarian cancer circulating tumor cell exosome in the background art has single function, can carry out centrifugal separation after cell solution is shaken up manually, and has certain limitation in use.
(II) technical scheme
In order to achieve the purpose, the utility model is realized by the following technical scheme:
a separation device for ovarian cancer circulating tumor cell exosomes comprises a bottom plate, wherein a shaking device is arranged above the bottom plate, and a containing device is arranged above the shaking device;
the left side and the right side of the upper surface of the bottom plate are both fixed with stand columns, and one ends of the stand columns, which are far away from the bottom plate, are fixed with first connecting pieces;
the shaking device comprises a motor, a rotating rod is fixed at the output end of the motor, a turntable is fixed at one end of the rotating rod, which is far away from the motor, a sliding column is fixed on the left side of the upper surface of the turntable, a base is arranged above the sliding column, a sliding groove is formed in the lower surface of the base, a supporting rod is fixed in the middle of the upper surface of the base, and second connecting pieces are fixed on the left side and the right side of the supporting rod;
the holding device comprises a holding plate, the lower surface of the holding plate is fixed to the upper surface of a supporting rod, a first placing groove is formed in the inner bottom wall of the holding plate, a cover plate is fixed to the upper surface of the holding plate, a second placing groove penetrating through the cover plate is formed in the cover plate, and the same test tube is arranged in the second placing groove and the first placing groove.
As a preferred technical scheme of the utility model: one side that the stand was kept away from to first connecting piece articulates there is the connecting rod, the one end that first connecting piece was kept away from to the connecting rod is articulated with the second connecting piece, can play certain limiting displacement to the bracing piece through the connecting rod, avoids when rocking, and the base breaks away from with the slip post, makes equipment stability more.
As a preferred technical scheme of the utility model: the top of test tube is provided with the rubber buffer, the surface of rubber buffer and the inner wall contact of test tube can play the guard action to the liquid in the test tube like this, avoid when rocking, the condition that the liquid in the test tube spills.
Furthermore, the motor is located above the bottom plate, and the lower surface of the motor is fixed with the upper surface of the bottom plate.
Further, the sliding tray sets up to annular spout, the sliding tray sets up for the slope, the sliding tray sets up to by the depth to shallow, the height that highly is greater than the sliding tray of slip post, and the surface of slip post and the inner wall contact of sliding tray, leave the clearance between base and the carousel.
Further, the first placing grooves are arranged in a plurality of numbers, the first placing grooves are distributed on the inner bottom wall of the placing disc in an annular array mode, the number of the second placing grooves is equal to the number of the first placing grooves, the positions of the second placing grooves correspond to the positions of the first placing grooves, and the diameters of the second placing grooves are equal to the diameters of the first placing grooves.
Further, the lower extreme surface of test tube and the inner wall contact of first standing groove, the upper end surface of test tube and the inner wall contact of second standing groove.
Theory of operation, this separator of ovarian cancer circulating tumor cell exosome, when needs use, at first put into the bottom that makes the test tube from the second standing groove with the test tube and contact with first standing groove, starter motor then, drive the rotary rod, the rotation of carousel and slip post, because slip post and sliding tray sliding connection, the slip post removes along with the depth change of sliding tray, thereby drive the bracing piece and place the dish and rock in a definite angle slope, to the liquid in the test tube rock evenly can.
The electrical components presented therein may be electrically connected to an external controller, and the controller may be electrically connected to an external 220V commercial power, and the controller may be a conventional known device for controlling a computer or the like.
(III) advantageous effects
The utility model provides a separation device of ovarian cancer circulating tumor cell exosomes, which has the following beneficial effects:
(1) this separator of ovarian cancer circulating tumor cell exosome through the cooperation that sets up between motor, rotary rod, carousel, slip post, base and the sliding tray, can drive the bracing piece and place the dish and rock in a certain angle slope, consequently can shake the liquid in the test tube evenly to replace the manual work to shake even operation, improved work efficiency.
(2) This separator of ovarian cancer circulating tumor cell exosome through the cooperation that sets up first connecting piece, second connecting piece and connecting rod, can play certain limiting displacement to the bracing piece, avoids when rocking, and the base breaks away from with the sliding column, makes more stable of equipment, through setting up first standing groove and second standing groove, can carry on spacingly to the test tube, increases the stability of test tube.
Drawings
FIG. 1 is a schematic perspective view of an apparatus for separating an exosome from a circulating tumor cell of ovarian cancer according to the present invention;
FIG. 2 is a schematic front view of the structure of the device for separating the exosomes from the circulating tumor cells of the ovarian cancer;
FIG. 3 is a schematic sectional view of the placement plate of the device for separating the exosomes from the circulating tumor cells of ovarian cancer;
FIG. 4 is a schematic sectional view of the base of the device for separating the exosomes from the circulating tumor cells of ovarian cancer according to the present invention;
FIG. 5 is a sectional view schematically showing the structure of the test tube of the device for separating the exosomes from the circulating tumor cells of ovarian cancer.
In the figure: 1. a base plate; 2. a shaking device; 3. a containing device; 4. a column; 5. a first connecting member; 6. a connecting rod; 7. a motor; 8. rotating the rod; 9. a turntable; 10. a sliding post; 11. a base; 12. a sliding groove; 13. a support bar; 14. a second connecting member; 15. placing a tray; 16. a first placing groove; 17. a cover plate; 18. a second placing groove; 19. a test tube; 20. and a rubber plug.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to FIGS. 1-5, the present invention provides an apparatus for separating exosomes from circulating tumor cells of ovarian cancer;
according to the figure 1, the device comprises a bottom plate 1, wherein a shaking device 2 is arranged above the bottom plate 1, and a containing device 3 is arranged above the shaking device 2;
according to the illustration of fig. 2, the left and right sides of the upper surface of the bottom plate 1 are both fixed with upright posts 4, and one end of each upright post 4 far away from the bottom plate 1 is fixed with a first connecting piece 5;
according to fig. 1 to 3, the containing device 3 comprises a placing disc 15, the lower surface of the placing disc 15 is fixed with the upper surface of the supporting rod 13, the inner bottom wall of the placing disc 15 is provided with a plurality of first placing grooves 16, the plurality of first placing grooves 16 are distributed on the inner bottom wall of the placing disc 15 in an annular array, a plurality of test tubes 19 can be placed by arranging the plurality of first placing grooves 16, so that the working efficiency is improved, the upper surface of the placing disc 15 is fixed with a cover plate 17, the cover plate 17 is provided with second placing grooves 18 penetrating through the cover plate 17, the number of the second placing grooves 18 is equal to the number of the first placing grooves 16, the positions of the second placing grooves 18 correspond to the positions of the first placing grooves 16, the diameter of the second placing grooves 18 is equal to the diameter of the first placing grooves 16, the same test tube 19 is arranged in the second placing grooves 18 and the first placing grooves 16, the outer surface of the lower end of the test tube 19 is in contact with the inner wall of the first placing groove 16, and the outer surface of the upper end of the test tube 19 is in contact with the inner wall of the second placing groove 18;
according to the figures 1-4, the shaking device 2 comprises a motor 7, the motor 7 is located above the bottom plate 1, the lower surface of the motor 7 is fixed with the upper surface of the bottom plate 1, a rotating rod 8 is fixed at the output end of the motor 7, a rotating disc 9 is fixed at one end of the rotating rod 8 far away from the motor 7, a sliding column 10 is fixed at the left side of the upper surface of the rotating disc 9, the height of the sliding column 10 is larger than that of a sliding groove 12, the outer surface of the sliding column 10 is in contact with the inner wall of the sliding groove 12, a base 11 is arranged above the sliding column 10, a gap is left between the base 11 and the rotating disc 9, so that a certain distance is kept between the base 11 and the rotating disc 9, the base 11 can be conveniently tilted and shaken up and down at a certain angle, a sliding groove 12 is formed in the lower surface of the base 11, the sliding groove 12 is arranged as an annular sliding groove, the sliding groove 12 is arranged obliquely, the sliding groove 12 is arranged from deep to shallow, the support rod 13 and the placing disc 15 can be driven to obliquely shake within a certain angle, so that liquid in the test tube 19 can be shaken uniformly, the support rod 13 is fixed in the middle of the upper surface of the base 11, and the second connecting pieces 14 are fixed on the left side and the right side of the support rod 13;
according to the illustration of fig. 2, as a preferred embodiment of the present invention: one side of the first connecting piece 5, which is far away from the upright post 4, is hinged with a connecting rod 6, one end of the connecting rod 6, which is far away from the first connecting piece 5, is hinged with a second connecting piece 14, and the connecting rod 6 can play a certain limiting role on the supporting rod 13, so that the base 11 is prevented from being separated from the sliding column 10 during shaking, and the equipment is more stable;
according to fig. 5, as a preferred embodiment of the present invention: the top of test tube 19 is provided with rubber buffer 20, and rubber buffer 20's surface and test tube 19's inner wall contact can play the guard action to the liquid in the test tube 19 like this, avoid when rocking, the condition that the liquid in the test tube 19 spills.
It is to be understood that, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," "secured," and the like are intended to be construed broadly and can include, for example, fixed and removable connections; or indirectly through an intermediary. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (7)
1. A separation device of ovarian cancer circulating tumor cell exosomes comprises a bottom plate (1), and is characterized in that a shaking device (2) is arranged above the bottom plate (1), and a containing device (3) is arranged above the shaking device (2);
the left side and the right side of the upper surface of the bottom plate (1) are both fixed with upright posts (4), and one end of each upright post (4) far away from the bottom plate (1) is fixed with a first connecting piece (5);
the shaking device (2) comprises a motor (7), a rotating rod (8) is fixed at the output end of the motor (7), a rotary table (9) is fixed at one end, far away from the motor (7), of the rotating rod (8), a sliding column (10) is fixed on the left side of the upper surface of the rotary table (9), a base (11) is arranged above the sliding column (10), a sliding groove (12) is formed in the lower surface of the base (11), a supporting rod (13) is fixed in the middle of the upper surface of the base (11), and second connecting pieces (14) are fixed on the left side and the right side of the supporting rod (13);
the containing device (3) comprises a containing disc (15), the lower surface of the containing disc (15) is fixed to the upper surface of a supporting rod (13), a first containing groove (16) is formed in the inner bottom wall of the containing disc (15), a cover plate (17) is fixed to the upper surface of the containing disc (15), a second containing groove (18) penetrating through the cover plate (17) is formed in the cover plate (17), and the same test tube (19) is arranged in the second containing groove (18) and the first containing groove (16).
2. An ovarian cancer circulating tumor cell exosome separation device according to claim 1, wherein one side of the first connecting piece (5) far away from the stand column (4) is hinged with a connecting rod (6), and one end of the connecting rod (6) far away from the first connecting piece (5) is hinged with the second connecting piece (14).
3. An ovarian cancer circulating tumor cell exosome separation device according to claim 1, characterized in that a rubber plug (20) is arranged above the test tube (19), and the outer surface of the rubber plug (20) is in contact with the inner wall of the test tube (19).
4. The device for separating the exosomes of the circulating tumor cells of the ovarian cancer according to claim 1, wherein the motor (7) is positioned above the bottom plate (1), and the lower surface of the motor (7) is fixed with the upper surface of the bottom plate (1).
5. The device for separating the exosomes of circulating tumor cells of ovarian cancer according to claim 1, wherein the sliding groove (12) is configured as an annular sliding groove, the sliding groove (12) is configured as an inclined groove, the sliding groove (12) is configured from deep to shallow, the height of the sliding column (10) is greater than that of the sliding groove (12), the outer surface of the sliding column (10) is in contact with the inner wall of the sliding groove (12), and a gap is reserved between the base (11) and the rotating disk (9).
6. An ovarian cancer circulating tumor cell exosome separation device according to claim 1, wherein a plurality of first placement grooves (16) are provided, the plurality of first placement grooves (16) are distributed on the inner bottom wall of the placement tray (15) in an annular array, the number of second placement grooves (18) is equal to the number of first placement grooves (16), the positions of the second placement grooves (18) correspond to the positions of the first placement grooves (16), and the diameter of the second placement grooves (18) is equal to the diameter of the first placement grooves (16).
7. An apparatus for isolating circulating tumor cell exosomes according to claim 1, wherein the lower outer surface of the test tube (19) is in contact with the inner wall of the first placement groove (16), and the upper outer surface of the test tube (19) is in contact with the inner wall of the second placement groove (18).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121192962.3U CN215365735U (en) | 2021-05-31 | 2021-05-31 | Separation device for ovarian cancer circulating tumor cell exosomes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121192962.3U CN215365735U (en) | 2021-05-31 | 2021-05-31 | Separation device for ovarian cancer circulating tumor cell exosomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215365735U true CN215365735U (en) | 2021-12-31 |
Family
ID=79632226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121192962.3U Active CN215365735U (en) | 2021-05-31 | 2021-05-31 | Separation device for ovarian cancer circulating tumor cell exosomes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215365735U (en) |
-
2021
- 2021-05-31 CN CN202121192962.3U patent/CN215365735U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN215365735U (en) | Separation device for ovarian cancer circulating tumor cell exosomes | |
| CN213967182U (en) | Animal serum separator | |
| CN220050203U (en) | Fixed-distance cutting structure for metal plate | |
| CN213210106U (en) | Drinking water quality detection device | |
| CN220056779U (en) | Tumor exosome separator | |
| CN221528148U (en) | Sample self-cleaning separator | |
| CN112077069B (en) | Cleaning equipment for cleaning semiconductor graphite and cleaning method thereof | |
| CN211784666U (en) | Food detects uses extraction processing apparatus | |
| CN209452054U (en) | A kind of centrifuge | |
| CN114354318A (en) | Clinical laboratory's blood analytical equipment | |
| CN220367059U (en) | Sampling device of chinese-medicinal material extract | |
| CN213835275U (en) | Separation device for ovarian cancer circulating tumor cell exosomes | |
| CN212840155U (en) | Novel multi-functional automatic thermal dimension stability tester | |
| CN216727687U (en) | Tiltable magnetic separation frame | |
| CN215140028U (en) | Sample partial shipment device for food inspection | |
| CN213255118U (en) | Platelet-rich plasma separation device | |
| CN219356560U (en) | Platelet-rich plasma PRP separation preparation facilities | |
| CN220131391U (en) | Uniform feeding device for lithium iron phosphate processing | |
| CN219348388U (en) | Bipartite operation platform and bipartite | |
| CN114130443B (en) | Movable physical and chemical inspection analysis test bed | |
| CN221383360U (en) | Automatic cake stirrer | |
| CN220443859U (en) | Biological asphalt laboratory heating device | |
| CN215640455U (en) | Centrifugal device for extracting cell DNA | |
| CN220460716U (en) | Instrument for extracting separating funnel | |
| CN214599788U (en) | High efficiency type plasma centrifuge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |