CN220056779U

CN220056779U - Tumor exosome separator

Info

Publication number: CN220056779U
Application number: CN202321571136.9U
Authority: CN
Inventors: 李双; 郭春燕; 刘建玲; 刘心星; 张佳燚; 孙璐瑶; 刘喜富; 刘风清; 王宁眉
Original assignee: Zhangjiakou Jianyuan Precision Medicine Co ltd; Hebei Normal University
Current assignee: Zhangjiakou Jianyuan Precision Medicine Co ltd; Hebei Normal University
Priority date: 2023-06-20
Filing date: 2023-06-20
Publication date: 2023-11-21
Anticipated expiration: 2033-06-20

Abstract

The utility model relates to the technical field of separation devices, in particular to a tumor exosome separation device, which comprises a chassis, wherein a driving motor is fixedly arranged on the upper surface of the chassis, a connecting plate is fixedly arranged at the output end of the driving motor, and shaking components for shaking a test tube are arranged at two ends of the connecting plate; the shaking component comprises a placing frame fixedly arranged at the end part of the connecting plate and a placing block rotatably arranged on the inner wall of the placing frame, two groups of placing grooves used for placing test tubes are formed in the upper surface of the placing block at equal intervals, and a positioning assembly used for positioning the test tubes is arranged on the upper surface of the placing block. According to the utility model, the shaking component and the positioning component are arranged, so that the purpose of effectively and uniformly mixing the liquid in the test tube by shaking the test tube can be realized when the device separates the tumor exosome, and the defect that the test tube cannot be effectively positioned in the prior art is effectively overcome.

Description

Tumor exosome separator

Technical Field

The utility model relates to the technical field of separation devices, in particular to a tumor exosome separation device.

Background

Exosomes refer to small vesicles (30-150 nm) containing complex RNAs and proteins, which nowadays are specifically disc-shaped vesicles with diameters of 40-100 nm. In the separation of exosomes, differential ultracentrifugation, density gradient ultracentrifugation, and the like are common methods.

Among the prior art, the separator of ovarian cancer circulation tumor cell exosome that publication number is CN215365735U, comprising a base plate, the top of bottom plate is provided with shakes the device, it is provided with holds the device to shake the top of device, the upper surface left and right sides of bottom plate all is fixed with the stand, the one end that the bottom plate was kept away from to the stand is fixed with first connecting piece, it includes the motor to shake the device, the output of motor is fixed with the rotary rod, the one end that the motor was kept away from to the rotary rod is fixed with the carousel, the upper surface left side of carousel is fixed with the slip post.

Above-mentioned patent technical scheme through setting up the cooperation between motor, rotary rod, carousel, slip post, base and the sliding tray, can drive the bracing piece and place the dish and shake at certain angle internal inclination, consequently can shake even to the liquid in the test tube to replace the manual work to shake even operation, improved work efficiency. But its in-process that shakes to the liquid in the test tube in reality is put into the bottom that makes the test tube from the second standing groove and contacts with first standing groove, and it carries out effective spacing to the test tube under the effect of first standing groove and second standing groove, but at the in-process that shakes, the circumstances that the test tube slides the collision from top to bottom appears very easily and throws away even appears, and the defect that can't carry out effective location to the test tube exists promptly in above-mentioned patent technology.

In view of the above, the utility model provides a tumor exosome separation device.

Disclosure of Invention

The utility model aims to provide a tumor exosome separation device to solve the problems in the background technology.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the tumor exosome separating device comprises a chassis, wherein a driving motor is fixedly arranged on the upper surface of the chassis, a connecting plate is fixedly arranged at the output end of the driving motor, and shaking components for shaking a test tube are arranged at two ends of the connecting plate;

the shaking assembly comprises a placing frame fixedly arranged at the end part of the connecting plate and a placing block rotatably arranged on the inner wall of the placing frame, two groups of placing grooves used for placing test tubes are formed in the upper surface of the placing block at equal intervals, and a positioning assembly used for positioning the test tubes is arranged on the upper surface of the placing block;

the locating component comprises a lifting plate which is arranged on the upper surface of the placement frame in a sliding manner, and an elastic rubber pad which is fixedly arranged on the lower surface of the lifting plate and used for propping up the top end of the test tube, and further comprises a telescopic column which is fixedly arranged on the lower surface of the lifting plate, and a reset spring which is sleeved on the surface of the telescopic column, wherein two ends of the reset spring are fixedly connected with the lower surface of the lifting plate and the upper surface of the placement block respectively.

As the preferable technical scheme, the inner wall of placing the frame rotates and is provided with the dwang, and place the piece and set firmly in the surface of dwang, the one end of dwang extends to the surface of placing the frame and sets firmly the rolling disc that is used for rotating to place the piece.

As the preferable technical scheme, the number of the telescopic columns is two, and the upper surface of the placement block is provided with a cylindrical groove which is in sliding connection with the surfaces of the two telescopic columns.

As the preferable technical scheme, the inner wall of the placing groove is provided with a protective sleeve for protecting the test tube, and the protective sleeve is made of elastic silica gel.

As the preferable technical scheme, the upper surface of lifter plate is provided with the handle of being convenient for upwards pulling to the lifter plate, and the surface cover of handle is equipped with anti-skidding rubber sleeve.

As the preferable technical scheme, the lower surfaces of the two placing frames are fixedly provided with limiting slide columns, and the upper surface of the chassis is provided with an annular chute for the two limiting slide columns to slide.

As the preferable technical scheme, the two groups of placing grooves are all obliquely arranged on the upper surface of the placing block, and the inclination angle of the placing grooves is 70 degrees.

Compared with the prior art, the utility model has the following beneficial effects:

1. according to the utility model, the shaking component and the positioning component are arranged, so that the purpose of effectively and uniformly mixing the liquid in the test tube can be realized when the device separates the tumor exosome, and the defect that the test tube cannot be effectively positioned in the prior art is effectively overcome, and the stability of the test tube is effectively improved.

2. According to the utility model, the chassis, the driving motor and the connecting plate are arranged, so that the purposes of shaking up before separation of exosomes and centrifuging after shaking up can be realized in the actual use process of the device, shaking up and centrifuging are integrated, and convenience is provided for operators.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a schematic diagram of a wobble assembly according to the present utility model;

FIG. 3 is a schematic cross-sectional view of a placement block according to the present utility model;

FIG. 4 is a schematic view of the internal structure of the placement tank of the present utility model.

In the figure: 1. a chassis; 2. a driving motor; 3. a connecting plate; 4. a test tube; 5. shaking the assembly; 501. placing a frame; 502. placing a block; 503. a placement groove; 504. a rotating disc; 505. a protective sleeve; 6. a positioning assembly; 601. a lifting plate; 602. an elastic rubber pad; 603. a telescopic column; 604. a return spring; 605. a cylindrical groove; 606. a handle; 7. and a limit sliding column.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

In order to better understand the above technical solutions, the following description will refer to the drawings and specific embodiments.

According to the embodiment of the utility model, as shown in the accompanying drawings 1-4, a tumor exosome separating device is provided, a chassis 1 is provided, a driving motor 2 is fixedly arranged on the upper surface of the chassis 1, a connecting plate 3 is fixedly arranged at the output end of the driving motor 2, and shaking components 5 for shaking a test tube 4 are arranged at two ends of the connecting plate 3;

the rocking assembly 5 comprises a placing frame 501 fixedly arranged at the end part of the connecting plate 3 and a placing block 502 rotatably arranged on the inner wall of the placing frame 501, wherein two groups of placing grooves 503 used for placing the test tubes 4 are formed in the upper surface of the placing block 502 at equal intervals, a protective sleeve 505 used for protecting the test tubes 4 is arranged on the inner wall of the placing groove 503, and elastic silica gel is used as a material of the protective sleeve 505.

The inner wall rotation of placing frame 501 is provided with the dwang, and places piece 502 and set firmly in the surface of dwang, and the one end of dwang extends to the surface of placing frame 501 and sets firmly the rolling disc 504 that is used for rotating to place piece 502.

And the upper surface of the placing block 502 is provided with a positioning component 6 for positioning the test tube 4;

the positioning assembly 6 comprises a lifting plate 601 which is arranged on the upper surface of the placement frame 501 in a sliding manner, a handle 606 which is convenient for pulling the lifting plate 601 upwards is arranged on the upper surface of the lifting plate 601, and an anti-slip rubber sleeve is sleeved on the surface of the handle 606.

And an elastic rubber pad 602 fixedly arranged on the lower surface of the lifting plate 601 and used for propping against the top end of the test tube 4, the positioning assembly 6 further comprises a telescopic column 603 fixedly arranged on the lower surface of the lifting plate 601, and a reset spring 604 sleeved on the surface of the telescopic column 603, and two ends of the reset spring 604 are fixedly connected with the lower surface of the lifting plate 601 and the upper surface of the placement block 502 respectively.

The number of the telescopic columns 603 is two, and cylindrical grooves 605 which are connected with the surfaces of the two telescopic columns 603 in a sliding manner are formed in the upper surface of the placement block 502.

According to the utility model, the shaking component 5 and the positioning component 6 are arranged, so that the device can be placed in the test tube 4 when separating a tumor exosome, the test tube 4 is inserted into the placing groove 503, meanwhile, the handle 606 is pulled upwards in the inserting process to drive the lifting plate 601 to move upwards, after the test tube 4 is inserted, the handle 606 is slowly loosened, at the moment, the lifting plate 601 can move downwards under the action of the reset spring 604 to effectively fix the test tube 4 in the placing groove 503, the effective positioning of the test tube 4 is realized, and the test tube 4 can be effectively protected under the action of the elastic rubber pad 602 and the protective sleeve 505, after the positioning is finished, a person can drive the placing block 502 to rotate through the rotating disc 504, the placing block 502 can rotate in the rotating process of the test tube 4, the purpose of effectively and evenly mixing liquid in the test tube 4 is realized, and the device effectively solves the defect that the test tube 4 cannot be effectively positioned in the prior art, and effectively improves the stability of the test tube 4.

The lower surfaces of the two placing frames 501 are fixedly provided with limiting slide posts 7, and the upper surface of the chassis 1 is provided with annular slide grooves for the two limiting slide posts 7 to slide.

The two groups of placing grooves 503 are all formed on the upper surface of the placing block 502 in an inclined mode, and the inclination angle of the placing grooves 503 is 70 degrees.

According to the utility model, after the test tube 4 is uniformly shaken by the shaking component 5, the device can start the driving motor 2 to drive the connecting plate 3 to rotate by arranging the chassis 1, the driving motor 2 and the connecting plate 3, and the rotation of the connecting plate 3 drives the two placing frames 501 to do circular motion around the output shaft of the driving motor 2, so that the aim of effectively centrifuging the test tube 4 on the two placing blocks 502 is fulfilled, and the inclined arrangement of the placing grooves 503 is convenient for collecting sediment at the bottom of the test tube 4 in the centrifuging process, so that the practicability is higher, and meanwhile, the stability of the placing frames 501 in circular motion can be effectively improved by arranging the limiting slide column 7 and the annular slide groove.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present utility model, and are not intended to limit the utility model, and that various changes and modifications may be made therein without departing from the spirit and scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. Tumor exosome separator, including chassis (1), its characterized in that: the upper surface of the chassis (1) is fixedly provided with a driving motor (2), the output end of the driving motor (2) is fixedly provided with a connecting plate (3), and both ends of the connecting plate (3) are provided with shaking components (5) for shaking the test tube (4);

the shaking assembly (5) comprises a placing frame (501) fixedly arranged at the end part of the connecting plate (3) and a placing block (502) rotatably arranged on the inner wall of the placing frame (501), two groups of placing grooves (503) for placing the test tube (4) are formed in the upper surface of the placing block (502) at equal intervals, and a positioning assembly (6) for positioning the test tube (4) is arranged on the upper surface of the placing block (502);

the locating component (6) comprises a lifting plate (601) which is arranged on the upper surface of the placement frame (501) in a sliding mode, and an elastic rubber pad (602) which is fixedly arranged on the lower surface of the lifting plate (601) and used for propping up the top end of the test tube (4), the locating component (6) further comprises a telescopic column (603) which is fixedly arranged on the lower surface of the lifting plate (601), and a reset spring (604) which is sleeved on the surface of the telescopic column (603), and two ends of the reset spring (604) are fixedly connected with the lower surface of the lifting plate (601) and the upper surface of the placement block (502) respectively.

2. The tumor exosome separation device according to claim 1, wherein: the inner wall rotation of placing frame (501) is provided with the dwang, and places piece (502) and set firmly in the surface of dwang, the one end of dwang extends to the surface of placing frame (501) and sets firmly the rolling disc (504) that are used for rotating to place piece (502).

3. The tumor exosome separation device according to claim 1, wherein: the number of the telescopic columns (603) is two, and cylindrical grooves (605) which are connected with the surfaces of the two telescopic columns (603) in a sliding manner are formed in the upper surface of the placement block (502).

4. The tumor exosome separation device according to claim 1, wherein: the inner wall of the placing groove (503) is provided with a protective sleeve (505) for protecting the test tube (4), and the protective sleeve (505) is made of elastic silica gel.

5. The tumor exosome separation device according to claim 1, wherein: the upper surface of lifter plate (601) is provided with handle (606) of being convenient for upwards pulling lifter plate (601), and the surface cover of handle (606) is equipped with anti-skidding rubber sleeve.

6. A tumor exosome separation apparatus according to any one of claims 1-5, wherein: the lower surfaces of the two placing frames (501) are fixedly provided with limiting slide posts (7), and the upper surface of the chassis (1) is provided with an annular chute for the two limiting slide posts (7) to slide.

7. A tumor exosome separation apparatus according to any one of claims 1-5, wherein: the two groups of placing grooves (503) are all obliquely arranged on the upper surface of the placing block (502), and the inclination angle of the placing grooves (503) is 70 degrees.