CN215328032U - Plant protease rapid separation purification device - Google Patents
Plant protease rapid separation purification device Download PDFInfo
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- CN215328032U CN215328032U CN202121574841.5U CN202121574841U CN215328032U CN 215328032 U CN215328032 U CN 215328032U CN 202121574841 U CN202121574841 U CN 202121574841U CN 215328032 U CN215328032 U CN 215328032U
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Abstract
The utility model discloses a plant protease rapid separation and purification device, which comprises a shell, a material collecting barrel and a separation part which are sequentially arranged from outside to inside, wherein the material collecting barrel is fixedly connected with the shell through a fixed rod, the output end of the material collecting barrel is communicated with the separation part through a liquid return pipe, a water supply tank is arranged outside the shell, and the material collecting barrel is communicated with the water supply tank; a waste recovery cylinder and a rotating motor are arranged below the separation part, the waste recovery cylinder is communicated with the separation part, and the output end of the rotating motor penetrates through the waste recovery cylinder to be in transmission connection with the separation part. The utility model can realize that the supernatant after centrifugation is quickly taken out and the centrifugation is repeated after washing, and in the process, the speed of manually taking out the supernatant and manually injecting water for washing is improved, thereby improving the purification and separation efficiency of the protease.
Description
Technical Field
The utility model relates to the technical field of protease treatment devices, in particular to a device for quickly separating and purifying plant protease.
Background
In the extraction process of the plant protease, firstly, plant tissues are crushed, then clear liquid is taken and added with a medicament, the liquid is introduced into an enzyme extraction device, and finally liquid with high protease content is obtained, and then the liquid with high protease content needs to be separated and purified to obtain the final protease.
At present, the separation and purification of protease mostly adopts superfine filter screen or filter paper to purify, though reached the separation and purification effect of protease, the liquid of high protease content is more, leads to purification speed slower, can also adopt centrifuge to carry out centrifugal separation in addition, takes out the supernatant after the centrifugal separation, wash again many times and come further purification protease, this process nevertheless needs the manual work to take out the supernatant and the washing of artifical water injection, has reduced the separation and purification efficiency of protease.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a plant protease rapid separation and purification device, which is used for solving the problems in the prior art, can realize rapid taking out of supernatant after centrifugation is finished, and can realize repeated centrifugal separation after washing.
In order to achieve the purpose, the utility model provides the following scheme: the utility model provides a plant protease rapid separation and purification device, which comprises a shell, a material collecting barrel and a separation part which are sequentially arranged from outside to inside, wherein the material collecting barrel is fixedly connected with the shell through a fixed rod, the output end of the material collecting barrel is communicated with the separation part through a liquid return pipe, a water supply tank is arranged outside the shell, and the material collecting barrel is communicated with the water supply tank;
a waste recovery cylinder and a rotating motor are arranged below the separation part, the waste recovery cylinder is communicated with the separation part, and the output end of the rotating motor penetrates through the waste recovery cylinder to be in transmission connection with the separation part;
the separation part include with the centrifuge bowl that the transmission of rotating electrical machines output is connected, a plurality of logical liquid hole and impurity export have been seted up on the centrifuge bowl, and is a plurality of logical liquid hole all is located the impurity export top, the centrifuge bowl passes through the impurity export with waste recovery section of thick bamboo intercommunication, logical liquid hole goes out liquid end intercommunication and has the drain pipe, the drain pipe goes out liquid end and is located the collection feed cylinder top, be provided with blocking member in the logical liquid hole, blocking member with centrifuge bowl sliding connection.
Preferably, an annular groove and a plurality of storage grooves are formed in the centrifugal cylinder, the annular groove is located above the plurality of storage grooves, the storage grooves are communicated with the liquid through hole, the blocking piece comprises an annular plate arranged in the annular groove, a plurality of first connecting rods are fixedly connected to the bottom end of the annular plate, the tail ends of the first connecting rods are located in the storage grooves, baffle plates are fixedly connected to the tail ends of the first connecting rods, and the baffle plates are located in the liquid through hole; the top end of the annular plate is fixedly connected with a second connecting rod, one end, far away from the annular plate, of the second connecting rod penetrates out of the top end of the centrifugal cylinder, and a handle is fixedly connected to the top end of the second connecting rod.
Preferably, the drain pipe sets up in the slope, just the drain pipe least significant end is higher than the aggregate cylinder highest end.
Preferably, a notch is formed in the liquid outlet end of the liquid outlet pipe, the notch is located at the bottom of the liquid outlet end, and the notch and the material collecting barrel are correspondingly arranged.
Preferably, the water supply tank is communicated with a water supply pipe, a water outlet end of the water supply pipe penetrates through the shell to be communicated with the material collecting barrel, and the water supply pipe and the liquid return pipe are both provided with driving pumps.
Preferably, an end cover is arranged in the centrifugal cylinder, and the end cover is in threaded connection with the centrifugal cylinder.
Preferably, the bottom end of the shell is fixedly connected with a support rod, and the top end of the support rod is fixedly connected with the waste recovery cylinder.
Preferably, a protease collecting box and a waste collecting box are arranged outside the shell, the protease collecting box is communicated with the material collecting barrel through a first collecting pipe, and the waste collecting box is communicated with the waste recycling barrel through a second collecting pipe.
The utility model discloses the following technical effects:
carry out centrifugal treatment to the protease turbid liquid through setting up the centrifuge bowl, the centrifugation back that finishes, the supernatant passes through the through-liquid hole and gets into the collection pipe, and in remaining impurity gets into the garbage collection section of thick bamboo, when the impurity in the centrifugal bowl of discharging, carries out the water injection in to the collection pipe through the feed water tank, treats the impurity discharge back of centrifuge bowl, carries out centrifugal treatment once more in the protease turbid liquid guide-in centrifuge bowl of collection section of thick bamboo through returning the liquid pipe, after the repeated operation many times, reaches the effect of protease purification.
In the protease purification process, the supernatant is easy to separate, and the supernatant after water injection is easy to enter the centrifugal cylinder again, so that the separation and purification efficiency of the protease is accelerated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic structural diagram of a plant protease rapid separation and purification device;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
FIG. 3 is a schematic view of the structure at B-B in FIG. 1;
figure 4 is a perspective view of the obturating member;
FIG. 5 is a perspective view of the drain tube;
the device comprises a shell, a material collecting barrel, a fixing rod, a liquid return pipe, a water supply tank, a waste recovery barrel, a rotating motor, a centrifugal barrel, a liquid through hole, a foreign matter outlet, a liquid outlet pipe, a 12 annular groove, a storage groove, a 14 annular plate, a first connecting rod, a 16 baffle, a second connecting rod, a handle, a 19 notch, a water supply pipe, a 21 driving pump, a 22 end cover, a support rod, a 24 protease collecting box, a waste collecting box, a first collecting pipe and a second collecting pipe, wherein the shell is 1, the material collecting barrel is 2, the fixing rod is 3, the liquid return pipe is 4, the water supply tank is 5, the waste recovery barrel is 6, the rotating motor is 7, the centrifugal barrel is 8, the liquid through hole is 9, the foreign matter outlet pipe is 10, the liquid outlet pipe is 12, the annular groove is 13, the storage groove is 14, the annular plate is 15, the first connecting rod, the baffle is 16, the second connecting rod, the handle is 19, the notch, the 21 driving pump, the support rod is 19, the support rod, the protease collecting box is 24 protease collecting box, the waste collecting box is 25, the waste collecting box is 26, the first collecting box, the second collecting pipe is 26, and the second collecting pipe is 27.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The utility model provides a plant protease rapid separation and purification device, which comprises a shell 1, a material collecting barrel 2 and a separation part which are sequentially arranged from outside to inside, wherein the material collecting barrel 2 is fixedly connected with the shell 1 through a fixing rod 3, the output end of the material collecting barrel 2 is communicated with the separation part through a liquid return pipe 4, a water supply tank 5 is arranged outside the shell 1, and the material collecting barrel 2 is communicated with the water supply tank 5; a waste recovery cylinder 6 and a rotating motor 7 are arranged below the separation part, the waste recovery cylinder 6 is communicated with the separation part, and the output end of the rotating motor 7 penetrates through the waste recovery cylinder 6 to be in transmission connection with the separation part; the separation portion includes the centrifuge tube 8 of being connected with the transmission of 7 output ends of rotating electrical machines, has seted up a plurality of logical liquid hole 9 and impurity export 10 on the centrifuge tube 8, and a plurality of logical liquid hole 9 all are located impurity export 10 top, and centrifuge tube 8 passes through impurity export 10 and waste recovery section of thick bamboo 6 intercommunication, and logical liquid hole 9 goes out the liquid end intercommunication and has drain pipe 11, and drain pipe 11 goes out the liquid end and is located the section of thick bamboo 2 tops that gathers materials, is provided with the plug member in the logical liquid hole 9, plug member and centrifuge tube 8 sliding connection.
Leading protease turbid liquid into a centrifugal cylinder 8, starting a rotating motor 7, driving the centrifugal cylinder 8 to rotate by the rotating motor 7, carrying out centrifugal treatment on the protease turbid liquid in the centrifugal cylinder 8, after the centrifugation is finished, opening a blocking piece, enabling supernatant to flow out of a liquid through hole 9, flowing into a material collecting cylinder 2 through a liquid outlet pipe 11, after the supernatant is led out, opening an impurity outlet 10, leading impurities in the centrifugal cylinder 8 into a waste material recovery cylinder 6, working a water supply tank 5 at the moment, injecting water into the material collecting cylinder 2, after the impurities in the centrifugal cylinder 8 are discharged, closing the impurity outlet 10, and leading the protease turbid liquid in the material collecting cylinder 2 into the centrifugal cylinder 8 again through a liquid return pipe 4, thereby carrying out centrifugal operation and completing the separation and purification of protease.
According to a further optimization scheme, an annular groove 12 and a plurality of storage grooves 13 are formed in the centrifugal cylinder 8, the annular groove 12 is located above the storage grooves 13, the storage grooves 13 are communicated with the liquid through hole 9, the blocking piece comprises an annular plate 14 arranged in the annular groove 12, the bottom end of the annular plate 14 is fixedly connected with a plurality of first connecting rods 15, the tail ends of the first connecting rods 15 are located in the storage grooves 13, the tail ends of the first connecting rods 15 are fixedly connected with baffle plates 16, and the baffle plates 16 are located in the liquid through hole 9; the top end of the annular plate 14 is fixedly connected with a second connecting rod 17, one end of the second connecting rod 17 far away from the annular plate 14 penetrates out of the top end of the centrifugal cylinder 8, and the top end of the second connecting rod 17 is fixedly connected with a handle 18.
When the supernatant is separated, the lifting handle 18 moves upwards, the lifting handle 18 drives the annular plate 14 to move upwards in the annular groove 12, the annular plate 14 drives the baffle 16 to enter the storage groove 13, at the moment, the baffle 16 does not block the liquid through hole 9, and therefore the supernatant can flow into the liquid outlet pipe 11 through the liquid through hole 9.
Because the liquid through hole 9 is provided with a plurality of, consequently drain pipe 11, first connecting rod 15, baffle 16 also are provided with a plurality ofly, and same annular plate 14 is connected to a plurality of first connecting rod 15, consequently handle 18 set up one can, when removing handle 18, a plurality of baffle 16 opens simultaneously or closes, has improved the separation speed of supernatant.
Further optimize the scheme, the slope of drain pipe 11 sets up, and 11 low ends of drain pipe are higher than the 2 highest ends of aggregate cylinder. The cleaning arrangement of the liquid outlet pipe 11 is convenient for supernatant to enter the material collecting barrel 2, and the bottommost end of the liquid outlet pipe 11 is not contacted with the highest end of the material collecting barrel 2, so that the centrifugal barrel 8 can normally rotate, and the protease suspension is centrifugally treated.
Further optimizing the scheme, the liquid outlet end of the liquid outlet pipe 11 is provided with a notch 19, the notch 19 is positioned at the bottom of the liquid outlet end, and the notch 19 is arranged corresponding to the material collecting barrel 2. The arrangement of the gap 19 makes the supernatant easier to enter the material collecting barrel 2, and reduces the possibility that the supernatant falls into the gap between the material collecting barrel 2 and the centrifugal barrel 8.
In a further optimized scheme, the water supply tank 5 is communicated with a water supply pipe 20, the water outlet end of the water supply pipe 20 penetrates through the shell 1 to be communicated with the material collecting barrel 2, and the water supply pipe 20 and the liquid return pipe 4 are both provided with a driving pump 21. The driving pump 21 is used for providing power for the liquid fluid, and meanwhile, the liquid circulation is controllable, the driving pump 21 can be manually controlled or electrically controlled, and the water supply pipe 20 and the liquid return pipe 4 are further provided with liquid flow control devices such as electromagnetic valves (not shown in the figure) to achieve the purpose of controlling the liquid flow.
In a further optimized scheme, an end cover 22 is arranged in the centrifugal cylinder 8, and the end cover 22 is in threaded connection with the centrifugal cylinder 8. The end cover 22 is used for preventing the protease suspension in the centrifuge bowl 8 from spilling during the centrifugation process, the end cover 22 is installed after the protease suspension is introduced into the centrifuge bowl 8, and the end cover 22 is opened after the centrifugation is finished, so that the subsequent re-centrifugation operation is facilitated.
Further optimize the scheme, the bottom of the shell 1 is fixedly connected with a support rod 23, and the top end of the support rod 23 is fixedly connected with the waste recovery cylinder 6. The support bar 23 is intended to support the waste recovery bowl 6, and the waste recovery bowl 6 should be positioned as close as possible to the bottom end of the bowl 8 to collect waste material, but not in contact with the bottom end of the bowl 8 to avoid affecting the normal rotation of the bowl 8.
According to a further optimized scheme, a protease collecting box 24 and a waste collecting box 25 are arranged outside the shell 1, the protease collecting box 24 is communicated with the material collecting barrel 2 through a first collecting pipe 26, and the waste collecting box 25 is communicated with the waste recycling barrel 6 through a second collecting pipe 27. After multiple times of washing and separation, high-purity protease is obtained, then the protease is guided into the protease collecting box 24 through the first collecting pipe 26, at the moment, less liquid still exists in the protease collecting box 24, and finally the target protease is obtained after treatment such as ultrafine filtration. And the waste material in the waste material recycling cylinder 6 enters the waste material collecting box 25 through the second collecting pipe 27, and the waste material in the waste material collecting box 25 still contains the protease, so the waste material can be separated according to actual needs, and the like, so that the extraction amount of the protease is improved.
The working principle is as follows:
the protease suspension is led into a centrifugal cylinder 8, a rotating motor 7 is started, the rotating motor 7 drives the centrifugal cylinder 8 to rotate, the protease suspension in the centrifugal cylinder 8 is subjected to centrifugal treatment, after the centrifugation is finished, a lifting handle 18 moves upwards, the lifting handle 18 drives an annular plate 14 to move upwards in an annular groove 12, the annular plate 14 drives a baffle 16 to enter a storage groove 13, the baffle 16 does not block a liquid through hole 9, therefore, supernatant can flow into a liquid outlet pipe 11 through the liquid through hole 9 and flows into a material collecting cylinder 2 from the liquid outlet pipe 11, after the supernatant is led out, an impurity outlet 10 is opened, impurities in the centrifugal cylinder 8 are led into a waste material recovery cylinder 6, at the moment, a water supply tank 5 works, water is injected into the material collecting cylinder 2, after impurities in the centrifugal cylinder 8 are discharged, the impurity outlet 10 is closed, the protease suspension in the material collecting cylinder 2 is led into the centrifugal cylinder 8 again through a liquid return pipe 4, and performing centrifugation again to complete the separation and purification of the protease. After multiple times of washing and separation, high-purity protease is obtained, then the protease is guided into the protease collecting box 24 through the first collecting pipe 26, at the moment, less liquid still exists in the protease collecting box 24, and finally the target protease is obtained after treatment such as ultrafine filtration. And the waste material in the waste material recycling cylinder 6 enters the waste material collecting box 25 through the second collecting pipe 27, and the waste material in the waste material collecting box 25 still contains the protease, so the waste material can be separated according to actual needs, and the like, so that the extraction amount of the protease is improved.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. A plant protease rapid separation and purification device is characterized by comprising a shell (1), a material collecting barrel (2) and a separation part which are sequentially arranged from outside to inside, wherein the material collecting barrel (2) is fixedly connected with the shell (1) through a fixing rod (3), the output end of the material collecting barrel (2) is communicated with the separation part through a liquid return pipe (4), a water supply tank (5) is arranged outside the shell (1), and the material collecting barrel (2) is communicated with the water supply tank (5);
a waste recovery cylinder (6) and a rotating motor (7) are arranged below the separation part, the waste recovery cylinder (6) is communicated with the separation part, and the output end of the rotating motor (7) penetrates through the waste recovery cylinder (6) to be in transmission connection with the separation part;
the separation portion include with centrifuge tube (8) that rotating electrical machines (7) output end transmission is connected, a plurality of logical liquid hole (9) and impurity export (10) have been seted up on centrifuge tube (8), and are a plurality of logical liquid hole (9) all are located impurity export (10) top, centrifuge tube (8) are passed through impurity export (10) with garbage recovery section of thick bamboo (6) intercommunication, logical liquid hole (9) play liquid end intercommunication has drain pipe (11), drain pipe (11) play liquid end is located aggregate section of thick bamboo (2) top, be provided with the shutoff piece in logical liquid hole (9), the shutoff piece with centrifuge tube (8) sliding connection.
2. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: an annular groove (12) and a plurality of storage grooves (13) are formed in the centrifugal cylinder (8), the annular groove (12) is located above the plurality of storage grooves (13), the storage grooves (13) are communicated with the liquid through holes (9), the blocking piece comprises an annular plate (14) arranged in the annular groove (12), a plurality of first connecting rods (15) are fixedly connected to the bottom end of the annular plate (14), the tail ends of the first connecting rods (15) are located in the storage grooves (13), a baffle (16) is fixedly connected to the tail ends of the first connecting rods (15), and the baffle (16) is located in the liquid through holes (9); the top of the annular plate (14) is fixedly connected with a second connecting rod (17), one end of the annular plate (14) is far away from the second connecting rod (17) and penetrates out of the top of the centrifugal cylinder (8), and a handle (18) is fixedly connected to the top of the second connecting rod (17).
3. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: drain pipe (11) slope sets up, just drain pipe (11) lowest end is higher than aggregate cylinder (2) highest end.
4. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: a notch (19) is formed in the liquid outlet end of the liquid outlet pipe (11), the notch (19) is located at the bottom of the liquid outlet end, and the notch (19) and the material collecting barrel (2) are arranged correspondingly.
5. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: the water supply tank (5) is communicated with a water supply pipe (20), the water outlet end of the water supply pipe (20) penetrates through the shell (1) to be communicated with the material collecting barrel (2), and the water supply pipe (20) and the liquid return pipe (4) are both provided with a driving pump (21).
6. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: an end cover (22) is arranged in the centrifugal cylinder (8), and the end cover (22) is in threaded connection with the centrifugal cylinder (8).
7. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: the bottom end of the shell (1) is fixedly connected with a supporting rod (23), and the top end of the supporting rod (23) is fixedly connected with the waste recovery cylinder (6).
8. The device for rapidly separating and purifying the plant protease as claimed in claim 1, wherein: the protease collecting box (24) and the waste collecting box (25) are arranged outside the shell (1), the protease collecting box (24) is communicated with the material collecting barrel (2) through a first collecting pipe (26), and the waste collecting box (25) is communicated with the waste recycling barrel (6) through a second collecting pipe (27).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121574841.5U CN215328032U (en) | 2021-07-12 | 2021-07-12 | Plant protease rapid separation purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121574841.5U CN215328032U (en) | 2021-07-12 | 2021-07-12 | Plant protease rapid separation purification device |
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| Publication Number | Publication Date |
|---|---|
| CN215328032U true CN215328032U (en) | 2021-12-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202121574841.5U Active CN215328032U (en) | 2021-07-12 | 2021-07-12 | Plant protease rapid separation purification device |
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| CN (1) | CN215328032U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114591832A (en) * | 2022-03-28 | 2022-06-07 | 四川大学 | Animal tissue aseptic decellularization device |
| CN116179337A (en) * | 2023-04-28 | 2023-05-30 | 东北农业大学 | Four-item separating device |
-
2021
- 2021-07-12 CN CN202121574841.5U patent/CN215328032U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114591832A (en) * | 2022-03-28 | 2022-06-07 | 四川大学 | Animal tissue aseptic decellularization device |
| CN116179337A (en) * | 2023-04-28 | 2023-05-30 | 东北农业大学 | Four-item separating device |
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