CN215263539U - Immunoreaction flow cell and immunoreaction system - Google Patents

Abstract

The application relates to an immunoreaction flow cell and an immunoreaction system, which relate to the technical field of detection equipment, and the immunoreaction flow cell of the application comprises: the gas barrier structure comprises a flow cell body, a liquid flow channel and at least one gas barrier structure, wherein the liquid flow channel is arranged on the flow cell body and is provided with two openings penetrating through the flow cell body; the air blocking structure is arranged on the inner wall of the liquid flow passage. Therefore, the air blocking structure is arranged on the inner wall of the liquid flow channel, so that air bubbles in the immunoreaction flow cell can be blocked through the air blocking structure, and the accuracy of measurement is improved.

Description

Immunoreaction flow cell and immunoreaction system

Technical Field

The application relates to the technical field of detection equipment, in particular to an immunoreaction flow cell and an immunoreaction system.

Background

Immune response detection is always an important research subject in biomedicine, and is also an important means in the fields of molecular biology, medical basic research, clinical diagnosis, new drug development, food health supervision, biological weapon war and the like. The requirement for immune response detection is specific quantitative detection, and the requirements for high detection sensitivity, small sample amount and rapid detection process are met.

The immunoreaction system in the prior art comprises a flow cell, detection equipment is arranged outside the flow cell, the flow cell is mostly in a common tubular structure, and the flow cell adopting the structure easily causes the problem of bubble generation in the flow cell, so that the measurement of an instrument is not accurate enough.

SUMMERY OF THE UTILITY MODEL

An object of the present application is to provide an immunoreaction flow cell and an immunoreaction system capable of blocking air bubbles in the immunoreaction flow cell by a gas blocking structure to improve the accuracy of measurement.

In order to achieve the above-mentioned objects,

in a first aspect, the present application provides an immunoreaction flow cell comprising: the gas barrier structure comprises a flow cell body, a liquid flow channel and at least one gas barrier structure, wherein the liquid flow channel is arranged on the flow cell body and is provided with two openings penetrating through the flow cell body; the air blocking structure is arranged on the inner wall of the liquid flow passage.

Wherein, the gas barrier structure is a groove structure.

In an embodiment, the flow cell body has a first surface and a second surface disposed opposite to each other, and the two openings are respectively disposed on the first surface and the second surface.

In an embodiment, a distance between the gas barrier structure and the first surface is greater than a distance between the gas barrier structure and the second surface.

In one embodiment, a pipe joint is connected to each of the two openings.

In one embodiment, the liquid flow channel is a straight hole channel, and the two openings are coaxially arranged.

In one embodiment, the liquid flow channel is a straight hole channel, and the two openings are arranged coaxially.

In one embodiment, the liquid flow channel is a curved channel, and the two openings are arranged coaxially.

In an embodiment, the liquid flow channel is a cylindrical pipe, the gas blocking structure is a groove structure, and a width of the gas blocking structure along a radial direction of the liquid flow channel may be equal to a diameter of the liquid flow channel.

In one embodiment, the flow cell body further has a third surface and a fourth surface disposed opposite to each other, and both the third surface and the fourth surface intersect the first surface; the distance between the liquid flow channel and the third surface is greater than the distance between the liquid flow channel and the fourth surface.

In an embodiment, the third surface is provided with a mounting groove communicated with the liquid flow channel, and a light-transmitting member is disposed in the mounting groove and made of a light-transmitting material.

In one embodiment, the flow cell body is made of a material having light transmittance.

In an embodiment, the flow cell body includes a first seat and a second seat connected to each other, the first seat is provided with a first half-hole channel, the second seat is provided with a second half-hole channel, and the first half-hole channel and the second half-hole channel form the liquid flow channel; the first seat is made of a material with light transmittance, and the second seat is made of a material with light shielding performance or a material with light transmittance.

In a second aspect, the present application provides an immune response system comprising: an immunoreaction flow cell, a magnetic separation device and an optical detection device, wherein the immunoreaction flow cell is the immunoreaction flow cell of any one embodiment; the magnetic separation device is arranged on the flow cell body; the optical detection device comprises a light emitting element, and light emitted by the light emitting element is configured to be emitted into the liquid flow channel. Wherein, the optical detection device and the magnetic separation device are respectively positioned at two sides of the immunoreaction flow cell.

In one embodiment, the magnetic separation device is disposed at a lower side of the flow cell body, and the optical detection device is disposed at an upper side of the flow cell body.

Compared with the prior art, the beneficial effect of this application is:

the utility model provides an immunoreaction flow cell and immunoreaction system sets up at least one and hinders the gas structure through setting up on the inner wall at liquid flow channel to can block the bubble in the immunoreaction flow cell through hindering the gas structure, with the accuracy that improves the measurement.

The utility model provides an immunoreaction flow cell and immunoreaction system is greater than the distance between liquid flow path and the fourth surface through making the distance between liquid flow path and the third surface to can be through the non-symmetry setting placed in the middle of liquid flow path, can adapt to magnetic separation device better, thereby can carry out the magnetic separation better.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

Fig. 1 is a schematic structural diagram of an immune response system according to an embodiment of the present disclosure.

Fig. 2 is a schematic perspective view of an immunoreaction flow cell according to an embodiment of the present application.

Fig. 3 is a top view of an immunoreaction flow cell of one embodiment of the present application.

Fig. 4 is a sectional view taken along a line a-a of fig. 3 according to an embodiment of the present application.

Fig. 5 is a cross-sectional view of an immunoreaction flow cell of one embodiment of the present application.

FIG. 6 is a cross-sectional view taken in the direction B-B of FIG. 3, illustrating one embodiment of the present application.

Fig. 7 is a cross-sectional view of an immunoreaction flow cell of one embodiment of the present application.

Fig. 8 is an exploded view of an immunoreaction flow cell according to an embodiment of the present application.

Icon: 90-immune response system; 91-magnetic separation means; 92-an optical detection device; 921-a light emitting member; 93-immunoreaction flow cell; 100-flow cell body; 101-a first surface; 102-a second surface; 103-a third surface; 104-a fourth surface; 110-a first seat; 111-a first half-bore; 120-a second seat; 121-a second half bore; 130-connecting hole; 200-a liquid flow channel; 211 — a first opening; 212-a second opening; 220-a pipe joint; 300-a gas barrier structure; 400-mounting a groove; 410-a light-transmitting member; 500-ring seal.

Detailed Description

The terms "first," "second," "third," and the like are used for descriptive purposes only and not for purposes of indicating or implying relative importance, and do not denote any order or order.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that the terms "inside", "outside", "left", "right", "upper", "lower", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally arranged when products of the application are used, and are used only for convenience in describing the application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the application.

In the description of the present application, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements.

The technical solution of the present application will be clearly and completely described below with reference to the accompanying drawings.

Please refer to fig. 1, which is a schematic structural diagram of an immunoreaction system 90 according to an embodiment of the present application. An immune response system 90 comprising: the device comprises an immunoreaction flow cell 93, a magnetic separation device 91 and an optical detection device 92, wherein the immunoreaction flow cell 93 comprises a flow cell body 100, and a liquid flow channel 200 is arranged on the flow cell body 100; the magnetic separation device 91 and the optical detection device 92 are respectively disposed on both sides of the flow cell body 100 for immunoreaction.

Wherein, the magnetic separation device 91 is arranged on the flow cell body 100 and is used for magnetic separation; the optical detection device 92 may be a CCD detection device (image pickup device) for performing optical detection, and the optical detection device 92 includes a light emitting member 921, and light emitted from the light emitting member 921 is configured to be incident into the liquid flow path 200.

Please refer to fig. 2, which is a schematic perspective view of an immunoreaction flow cell 93 according to an embodiment of the present application. The flow cell body 100 has a first surface 101 and a second surface 102 which are oppositely arranged, and the liquid flow channel 200 has two openings penetrating through the first surface 101 and the second surface 102 of the flow cell body 100; the two openings are a first opening 211 and a second opening 212, respectively, the first opening 211 is disposed on the first surface 101, and the second opening 212 is disposed on the second surface 102.

One of the first opening 211 and the second opening 212 is selected as a liquid inlet and the other is selected as a liquid outlet, as required. For example, when the reaction solution is aspirated for use as an immunological reaction, the second opening 212 may be used as a liquid inlet and the first opening 211 may be used as a liquid outlet; when the cleaning liquid is introduced for magnetic separation cleaning, the second opening 212 may be used as a liquid outlet, and the first opening 211 may be used as a liquid inlet.

At least one air blocking structure 300 is arranged on the inner wall of the liquid flow passage 200, and the distance between the air blocking structure 300 and the first surface 101 is larger than the distance between the air blocking structure 300 and the second surface 102. In this embodiment, the gas barrier structure is a groove structure, and one gas barrier structure 300 is disposed closer to the second surface 102 than the first surface 101, that is, the gas barrier structure 300 is closer to the second opening 212 than the first opening 211.

The flow cell body 100 further has oppositely disposed third and fourth surfaces 103, 104, the third and fourth surfaces 103, 104 each intersecting the first surface 101.

In this embodiment, the flow cell body 100 has a rectangular parallelepiped shape, and the third surface 103 and the fourth surface 104 are perpendicular to the first surface 101, and the first surface 101 and the second surface 102 are parallel. The first surface 101 is defined as the left surface of the flow cell body 100, the second surface 102 is defined as the right surface of the flow cell body 100, the third surface 103 is defined as the upper surface of the flow cell body 100, and the fourth surface 104 is defined as the lower surface of the flow cell body 100.

In the immunoreaction system 90 (please refer to fig. 1), the magnetic separation device 91 is attached to the fourth surface 104 of the flow cell body 100; the optical detection device 92 is disposed on a side of the flow cell body 100 facing away from the fourth surface 104, i.e., above the flow cell body 100. The flow cell body 100 is integrally formed, and the whole flow cell body 100 is made of a material with light transmittance, so that the optical detection device 92 can perform optical detection, and light emitted by the light emitting element 921 can be emitted into the liquid flow channel 200.

The first opening 211 and the second opening 212 are each connected to a pipe joint 220, the pipe joint 220 may be a screw joint, and the connection method of the pipe joint 220 and the flow cell body 100 is other connection methods such as a screw connection or an adhesive seal connection. The pipe joint 220 is arranged to facilitate the suction and outflow of liquid.

In one operation, when the reaction solution is sucked by the auxiliary tool such as the sampling needle, the auxiliary tool sucks a section of air column first, then sucks the reaction solution, and then sucks a section of air column for injecting the reaction solution into the immunoreaction flow cell 93.

Then, the reaction liquid in the auxiliary tool enters the liquid channel 200 from the second opening 212, and flows to the middle of the liquid channel 200 for detection. Since the air blocking structure 300 is disposed near the inlet, air can enter the air blocking structure 300 to block air bubbles before the reaction liquid enters the middle of the liquid channel 200, so as to improve the detection accuracy.

Wherein, when the reaction liquid flows from the second opening 212 to the middle of the liquid channel 200, the magnetic separation device 91 is attached to the fourth surface 104 to achieve magnetic separation. In the magnetic separation process, the cleaning solution enters the liquid channel 200 from the first opening 211 to remove the antigen and antibody which are not coated in the immune reaction, and the waste cleaning solution flows out from the second opening 212; after the magnetic separation is completed, the optical detection device 92 starts to detect; after the completion of the detection by the optical detection device 92, the immunoreaction flow cell 93 is cleaned, and when the immunoreaction flow cell 93 is cleaned, the operation is completed.

Please refer to fig. 3, which is a top view of an immunoreaction flow cell 93 according to an embodiment of the present application. The flow cell body 100 is provided with a plurality of connection holes 130, and the connection holes 130 may be stepped holes or threaded holes, which is beneficial to fixing the flow cell body 100. In this embodiment, four connection holes 130 are provided, and are respectively located at four corners of the flow cell body 100.

Please refer to fig. 4, which is a sectional view taken along a direction a-a of fig. 3 according to an embodiment of the present disclosure. The shape of the gas barrier structure 300 may be any one of a U shape, a rectangular parallelepiped shape, a square shape, a cylindrical shape, or a conical shape. The inner bottom surface of the gas barrier structure 300 may be curved or flat.

In this embodiment, the liquid flow channel 200 is a straight hole channel, and the first opening 211 and the second opening 212 are coaxially disposed. In another embodiment, the liquid flow channel 200 is a curved channel, and the axis of the first opening 211 is not coincident with the axis of the second opening 212. In another embodiment, the liquid flow path 200 is a straight hole path, and the axis of the first opening 211 and the axis of the second opening 212 are not coincident.

Please refer to fig. 5, which is a cross-sectional view of an immunoreaction flow cell 93 according to an embodiment of the present application. The distance d1 between the liquid flow path 200 and the third surface 103 is greater than the distance d2 between the liquid flow path 200 and the fourth surface 104. With such an arrangement, the liquid channel 200 is disposed asymmetrically, the liquid channel 200 is closer to the fourth surface 104 than the third surface 103, and the magnetic separation device 91 of the present application is attached to the fourth surface 104, so as to perform magnetic separation better.

To enhance the effect of blocking bubbles, the length d4 of the gas barrier structure 300 along the axial direction of the liquid flow passage 200 may be 2-4mm, and the depth d3 of the gas barrier structure 300 along the radial direction of the liquid flow passage 200 may be 0.5-3 mm.

For example, the length d4 of the gas barrier structure 300 in the axial direction of the liquid flow passage 200 is 2mm, 2.5mm, 3mm, 3.5mm or 4mm, and the depth d3 of the gas barrier structure 300 in the radial direction of the liquid flow passage 200 may be 0.5mm, 0.75mm, 0.8mm, 0.9mm, 1mm, 2mm or 3 mm.

Please refer to fig. 6, which is a cross-sectional view taken along the direction B-B of fig. 3 according to an embodiment of the present disclosure. The liquid flow passage 200 is a cylindrical pipe, the width d5 of the choke structure 300 along the radial direction of the liquid flow passage 200 may be equal to the diameter d6 of the liquid flow passage 200, and the depth d3 of the choke structure 300 along the radial direction of the liquid flow passage 200 may be less than or equal to the diameter d6 of the liquid flow passage 200, so that the effect of blocking bubbles may be improved.

The width d5 of the gas barrier structure 300 along the radial direction of the liquid flow passage 200 and the diameter d6 of the liquid flow passage 200 are both smaller than or equal to the lens visual field range of the CCD detection device (image pickup device).

Fig. 7 is a cross-sectional view of an immunoreaction flow cell 93 shown in an embodiment of the present application. The third surface 103 is provided with a mounting groove 400 communicating with the liquid flow channel 200, wherein the mounting groove 400 is disposed in the middle of the liquid flow channel 200, a light-transmitting member 410 is disposed in the mounting groove 400, and the light-transmitting member 410 is made of a material having light-transmitting property, so as to be used for optical detection of the optical detection device 92, and light emitted by the light-emitting member 921 can be incident into the liquid flow channel 200. The material of the light-transmitting member 410 may be transparent glass, transparent plastic, or quartz.

The portion of the flow cell body 100 other than the light transmitting member 410 may be made of a material having light transmittance or a material having light shielding property.

In this embodiment, the length of the mounting groove 400 along the axial direction of the liquid flow channel 200 is gradually decreased from top to bottom to improve the connection tightness between the light-passing element 410 and the flow cell body 100. The connection mode of the light-passing element 410 and the flow cell body 100 may be interference fit, clamping, welding, or threaded connection. In another embodiment, the light-transmitting member 410 and the flow cell body 100 are a unitary structure.

Please refer to fig. 8, which is an exploded view of an immunoreaction flow cell 93 according to an embodiment of the present application. The flow cell body 100 includes a first seat 110 and a second seat 120 which are connected with each other up and down, a first semi-pore passage 111 is provided on the first seat 110, a second semi-pore passage 121 is provided on the second seat 120, and the first semi-pore passage 111 and the second semi-pore passage 121 form a liquid flow channel 200.

The third surface 103 is disposed on the first seat 110, and the first seat 110 is made of a transparent material; the fourth surface 104 is disposed on the second seat 120, and the second seat 120 is made of a light-shielding material or a light-transmitting material for optical detection of the optical detection device 92, which is favorable for light emitted by the light emitting element 921 to enter the liquid flow channel 200.

The connection between the first and second sockets 110 and 120 may be a bolt connection or a welding connection. An annular seal 500 is provided between the first seat 110 and the second seat 120 to improve the sealing of the flow cell body 100. The annular sealing element 500 may be made of sealant or the like, i.e., the first seat 110 and the second seat 120 are connected by a bonding process.

It should be noted that the features of the embodiments in the present application may be combined with each other without conflict.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. An immunoreaction flow cell comprising:

a flow cell body;

the liquid flow channel is arranged on the flow cell body and is provided with two openings penetrating through the flow cell body; and

and the at least one air blocking structure is arranged on the inner wall of the liquid flow channel.

2. The immunoreactive flow cell of claim 1, wherein said flow cell body has a first surface and a second surface disposed opposite to each other, said two openings being disposed on said first surface and said second surface, respectively.

3. The immunoreaction flow cell of claim 2, wherein the distance between said gas barrier structure and said first surface is greater than the distance between said gas barrier structure and said second surface.

4. The immunoreaction flow cell of claim 2 wherein a tube connector is attached to each of said two openings.

5. The immunoreaction flow cell according to claim 2, wherein said liquid flow channel is a cylindrical tube, and said gas barrier structure is a groove structure, and a width of said gas barrier structure in a radial direction of said liquid flow channel is equal to a diameter of said liquid flow channel.

6. The immunoreactive flow cell of claim 2 wherein said flow cell body further has oppositely disposed third and fourth surfaces, said third and fourth surfaces each intersecting said first surface;

the distance between the liquid flow channel and the third surface is greater than the distance between the liquid flow channel and the fourth surface.

7. The immunoreaction flow cell of claim 6, wherein a mounting groove is formed on the third surface and is communicated with the liquid flow channel, and a light-transmitting member is arranged in the mounting groove and is made of a light-transmitting material.

8. The immunoreaction flow cell of any one of claims 1 to 6, wherein said flow cell body is made of a material having light transmittance.

9. The immunoreaction flow cell of any one of claims 1 to 6, wherein said flow cell body comprises a first seat and a second seat connected to each other, said first seat having a first half-bore and said second seat having a second half-bore, said first half-bore and said second half-bore forming said liquid flow channel;

the first seat is made of a material with light transmittance, and the second seat is made of a material with light shielding performance or a material with light transmittance.

10. An immune response system, comprising:

an immunoreaction flow cell according to any one of claims 1 to 9;

a magnetic separation device; the flow cell body is arranged; and

the optical detection device comprises a light emitting piece, wherein light emitted by the light emitting piece is configured to be emitted into the liquid flow channel;

wherein, the optical detection device and the magnetic separation device are respectively positioned at two sides of the immunoreaction flow cell.

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