CN218782186U - Flow chamber assembly, optical detection equipment and sample analyzer - Google Patents

Abstract

The application discloses a flow cell assembly, an optical detection device and a sample analyzer. The flow chamber assembly comprises a flow chamber and a connecting piece, the flow chamber is provided with a detection flow channel, the connecting piece is connected with the outer peripheral wall of the flow chamber and extends to the outer side of the flow chamber along the radial direction of the detection flow channel, the connecting piece is provided with a positioning surface parallel to the axial direction of the detection flow channel, and the positioning surface is used for positioning the flow chamber assembly when the flow chamber assembly is installed; the peripheral wall of the flow chamber is provided with a first observation area and a second observation area which are at least partially overlapped with the detection flow channel along the axial direction of the detection flow channel, the first observation area is used for allowing incident light to be transmitted from the outside of the flow chamber to the inside of the detection flow channel, the second observation area is used for allowing lateral emergent light formed after the incident light acts on sample particles to be transmitted from the inside of the detection flow channel to the outside of the flow chamber, and the connecting piece is arranged to avoid the first observation area and the second observation area. The mounting precision can be improved, and meanwhile, the sample to be detected can be conveniently detected.

Description

Flow chamber assembly, optical detection equipment and sample analyzer

Technical Field

The present application relates to the field of medical device technology, and more particularly, to a flow cell assembly, an optical detection apparatus, and a sample analyzer.

Background

The sample analyzer can comprise a cell analyzer, wherein the cell analyzer is one of the sample analyzers commonly used for medical detection, is an instrument for detecting parameters such as the number, the proportion and the like of blood cells (red blood cells, white blood cells and blood platelets) in blood, and realizes the functions of microbial infection type, anemia diagnosis and treatment, blood disease diagnosis and the like of a detected sample through the analysis of the blood. With the progress of technology and the development of science and technology, the functions of the sample analyzer are continuously expanded, the performance is continuously improved, the automation degree is continuously improved, and the sample analyzer is widely applied clinically.

The sample analyzer may include devices such as a flow chamber component, and a common sample analyzer (for example, a cell analyzer) is a device for performing classification and statistics on particles based on a flow cytometry, and the basic measurement principle is as follows: after being wrapped by sheath fluid, the processed sample particles (such as blood cells) pass through the flow chamber one by one under the push of pressure.

Because the volume of sample particle is less, can be detected smoothly in order to guarantee the sample particle that awaits measuring, the room that flows need carry out the position debugging in the installation to make the room that flows have higher installation accuracy, the room that flows has a plurality of removal and pivoted degrees of freedom before fixed, and the debugging dimension is more, influences the installation effectiveness, how to improve the installation accuracy from this in the time, can also be convenient for detect the sample that awaits measuring and become key concern problem.

SUMMERY OF THE UTILITY MODEL

It is a primary object of the present application to provide a flow cell assembly, an optical detection apparatus and a sample analyzer, which aim to solve the above technical problems existing in the prior art.

In order to solve the above problems, the present application provides a flow chamber assembly including a flow chamber provided with a detection flow channel for receiving a sheath flow containing sample particles and moving the sample particles in the sheath flow along an axial direction of the detection flow channel, and a connecting member connected to an outer peripheral wall of the flow chamber and extending outward of the flow chamber in a radial direction of the detection flow channel, the connecting member having a positioning surface parallel to the axial direction of the detection flow channel, the positioning surface being used for positioning the flow chamber assembly when the flow chamber assembly is installed; wherein the outer peripheral wall of the flow chamber is provided with a first observation region and a second observation region that at least partially overlap with the detection flow channel in the axial direction of the detection flow channel, the first observation region being configured to allow incident light to be transmitted from the outside of the flow chamber to the inside of the detection flow channel, the second observation region being configured to allow lateral outgoing light, which is formed by the incident light after acting on the sample particles, to be transmitted from the inside of the detection flow channel to the outside of the flow chamber, and the connecting member is configured to avoid the first observation region and the second observation region.

In an embodiment, the connecting member is connected to one side of the flow chamber, and one of the first observation region and the second observation region is disposed on the other side of the flow chamber opposite to the connecting member along the radial direction of the detection flow channel.

In one embodiment, at least a portion of the connecting member and one of the first observation area and the second observation area are located on the same side of the flow chamber, the flow chamber has an avoidance area which is formed along an axial direction of the inspection flow channel and avoids the connecting member, and one of the first observation area and the second observation area is arranged in the avoidance area.

In an embodiment, the other of the first and second observation areas is arranged parallel to the extending direction of the connecting member.

In an embodiment, the first observation area is arranged parallel to the extending direction of the connecting piece, the peripheral wall of the flow chamber is further provided with a third observation area arranged opposite to the first observation area, and the third observation area is used for allowing backward emergent light formed by the incident light after acting on the sample particles to be transmitted from the inside of the detection flow channel to the outside of the flow chamber.

In one embodiment, the connecting member is disposed to at least partially overlap with the detection flow channel in an axial direction of the detection flow channel.

In one embodiment, the connecting member is disposed in a plate shape, and the positioning surface is a main surface of the connecting member.

In one embodiment, the flow chamber and the connector are integrally formed, and the optical wall thickness of the flow chamber in the first and second fields of view is between 0.5mm and 4 mm.

In order to solve the above problems, the present application provides an optical inspection apparatus, which includes a light source assembly, an inspection assembly, and the above flow chamber assembly, wherein the light source assembly is configured to emit incident light to the inspection flow channel; the detection assembly is used for receiving emergent light formed after the incident light acts on the sample particles.

In order to solve the above problems, the present application provides a sample analyzer, comprising: the flow chamber assembly comprises a sampling module, a sample preparation module and the flow chamber assembly, wherein the sampling module is used for obtaining a sample, and the sample preparation module is used for receiving the sample and mixing the sample with a reagent to obtain the sample to be detected.

Compared with the prior art, the flow chamber assembly comprises a flow chamber and a connecting piece, wherein the flow chamber is provided with a detection flow channel, the detection flow channel is used for receiving sheath flow containing sample particles, so that the sample particles in the sheath flow move along the axial direction of the detection flow channel, the connecting piece is connected with the outer peripheral wall of the flow chamber and extends to the outer side of the flow chamber along the radial direction of the detection flow channel, the connecting piece is provided with a positioning surface parallel to the axial direction of the detection flow channel, and the positioning surface is used for positioning the flow chamber assembly when the flow chamber assembly is installed; wherein the peripheral wall of the flow chamber is provided with a first observation area and a second observation area which are at least partially overlapped with the detection flow channel along the axial direction of the detection flow channel, the first observation area is used for allowing incident light to be transmitted from the outside of the flow chamber to the inside of the detection flow channel, the second observation area is used for allowing lateral emergent light formed after the incident light acts on sample particles to be transmitted from the inside of the detection flow channel to the outside of the flow chamber, and the connecting piece is arranged to avoid the first observation area and the second observation area. Through above-mentioned embodiment, the flow chamber subassembly can be fixed a position with other parts through the locating surface, can also carry out optical detection to the sample particle through first observation area and second observation area simultaneously to can be when improving the installation accuracy, can also be convenient for detect the sample that awaits measuring.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an embodiment of an optical inspection apparatus provided herein;

FIG. 2 is a first perspective view of a schematic structural view of a first embodiment of a flow cell assembly provided herein;

FIG. 3 is a cut-away view of a schematic structural view of a first embodiment of a flow cell assembly provided herein;

FIG. 4 is a second perspective view of the schematic structural view of the first embodiment of the flow cell assembly provided herein;

FIG. 5 is a first perspective view of a schematic structural view of a second embodiment of a flow cell assembly provided herein;

FIG. 6 is a cut-away view of a schematic structural view of a second embodiment of a flow chamber assembly provided herein;

fig. 7 is a second perspective view of a schematic structural diagram of a second embodiment of a flow cell assembly as provided herein.

Reference numerals: an optical detection device 1; a flow chamber assembly 10; a light source assembly 20; a backlight assembly 30; a lateral assembly 40; a connecting member 100; a positioning surface 110; a flow chamber 200; a detection flow channel 210; a first observation zone 310; a second observation zone 320; a third observation zone 330; avoiding the area 400.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be further noted that, for the convenience of description, only some of the structures associated with the present application are shown in the drawings, not all of them. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.

The terms "first", "second" and "third" in this application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or as implying a number of indicated technical features. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of the feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless explicitly specified otherwise. In the embodiment of the present application, all directional indicators (such as up, down, left, right, front, rear \8230;) are used only to explain the relative positional relationship between the components, the motion situation, etc. at a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.

The application provides a sample analyzer, which is applied to the field of medical or biochemical analysis. For example, the sample analyzer may include a blood cell analyzer, and the sample analyzer may be other clinical laboratory equipment.

The sample analyzer may be used to perform fluorescence detection on the sample. The sample analyzer comprises a sampling module, a sample preparation module and a flow chamber assembly, wherein the sampling module is used for obtaining a sample, and the sample preparation module is used for receiving the sample and mixing the sample with a reagent to obtain a sample to be detected. The sample preparation module can further comprise a transportation module, a sampling module, a reagent module, a reaction module, a magnetic separation module and an optical detection device. When the device is used, the original sample is transferred to the sampling module through the transportation module, the sampling module is used for sampling the original sample quantitatively, and the original sample is transferred to the reaction module to wait for reaction. The reagent module is used for accommodating and preparing reagents required by sample detection, the transportation module transports the reagents which are required to be added into the samples to the reagent station, and the reagent needles of the reagent module absorb the corresponding reagents and accurately and quantitatively add the reagents into the reaction disc of the reaction module, wherein the reagents comprise magnetic beads with specific antibodies.

The sampling needle adds the original sample to the reaction disc, and in order to make the antigen in the original sample combine with the specific antibody in the reagent, the original sample needs to be incubated in the reaction disc for a period of time. After incubation, the magnetic separation module performs magnetic separation on the incubated sample mixture, cells which do not react with specific antibodies are separated in the magnetic separation module through an immunomagnetic bead separation technology, and magnetic bead compositions obtained through magnetic separation are cleaned, so that reagents which cannot be combined with magnetic beads in the reaction disc and other wastes are cleaned, and then a sample to be detected is obtained. The sample to be detected enters the flow chamber assembly, and in the process that sample particles of the sample to be detected pass through the detection flow channel of the flow chamber assembly one by one, the optical detection equipment detects the sample to be detected and converts photoelectric data to obtain detection data.

The application provides an optical detection device, and referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of the optical detection device provided in the application. The optical inspection apparatus 1 may further include a light source assembly 20, an inspection assembly, and a flow cell assembly 10, the light source assembly 20 being configured to emit incident light to the inspection flow channel 210; the detection assembly is used for receiving emergent light formed after the incident light acts on the sample particles. Wherein, the detection assembly may include a lateral assembly 40 and a backlight assembly 30, the light source assembly 20, the flow cell assembly 10 and the backlight assembly 30 are sequentially disposed on the optical path of the incident light, and the lateral assembly 40 is located at one side of the flow cell assembly 10. The light source assembly 20 emits incident light to sample particles of the flow chamber assembly 10, the sample particles convert the incident light into forward emergent light and lateral emergent light, the forward emergent light is received by the rear light assembly 30, the lateral emergent light is received by the lateral assembly 40, and therefore a sample to be detected is analyzed according to light signals received by the lateral assembly 40 and light signals received by the rear light assembly 30, and a detection result is finally obtained.

In the in-process that detects, because the volume of sample particle is less, can be detected smoothly in order to guarantee the sample particle that awaits measuring, flow room 200 need carry out the position debugging in the installation to make flow room 200 have higher installation accuracy, flow room 200 has a plurality of removal and pivoted degrees of freedom before fixed, the debugging dimension is more, influence the installation effectiveness, how to improve the installation accuracy from this, can also be convenient for detect the sample that awaits measuring and become key concern problem.

To solve the above technical problem, the present application further provides a flow cell assembly 10, see fig. 1-7.

The flow chamber assembly 10 comprises a flow chamber 200 and a connecting member 100, the flow chamber 200 is provided with a detection flow channel 210, and the detection flow channel 210 is used for receiving a sheath flow containing sample particles, so that the sample particles in the sheath flow move along the axial direction of the detection flow channel 210. The connection member 100 is connected to the outer peripheral wall of the flow cell 200 and extends outward of the flow cell 200 in the radial direction of the detection flow channel 210, and the connection member 100 has a positioning surface 110 parallel to the axial direction of the detection flow channel 210, the positioning surface 110 being used to position the flow cell assembly 10 when the flow cell assembly 10 is mounted to other components.

The sheath flow can be obtained by mixing sheath liquid and a sample to be detected, specifically, the sheath liquid can be provided by a sheath liquid module of a sample analyzer, the flow chamber 200 is connected with the sheath liquid module through a pipeline, and the sheath liquid enters the internal cavity of the flow chamber 200 through a sheath liquid inlet, and after entering the internal cavity of the flow chamber 200, the sheath liquid is in a laminar flow state in the cavity of the flow chamber 200; the sample to be detected enters the cavity of the flow chamber 200 from the sample inlet of the flow chamber 200, after the sample to be detected enters the cavity, the sheath fluid can stably and uniformly pass through the sheath fluid, the sheath fluid plays a good role in wrapping and bounding the flowing sample, so that the sheath fluid and the sample form a sheath flow together, the sheath flow continues to flow along the direction of the detection flow channel 210 of the flow chamber 200, so that the detection flow channel 210 receives the sheath flow, and the sample particles in the sheath flow move along the axial direction of the detection flow channel 210, and in the process that the sample particles pass through the detection flow channel 210, the sample to be detected is optically detected through the light source assembly 20 and the detection assembly.

The connecting member 100 is connected to the outer peripheral wall of the flow chamber 200 and extends radially outward of the flow chamber 200 along the detection flow channel 210, that is, the connecting member 100 may be sleeved outside the outer peripheral wall of the flow chamber 200 and gradually extends outward of the outer peripheral wall of the flow chamber 200, so that the connecting member 100 forms a positioning surface 110 parallel to the axial direction of the detection flow channel 210, and the positioning surface 110 may be used to position the flow chamber assembly 10 when the flow chamber assembly 10 is mounted to other components. Other components may include the light source assembly 20 and/or the backlight assembly 30, that is, the positioning surface 110 may position the flow chamber assembly 10 at the light source assembly 20 and/or the backlight assembly 30, so that the flow chamber assembly 10 and the light source assembly 20 and/or the backlight assembly 30 may have a mutual limiting effect on the relative position, and the flow chamber assembly 10 does not need to be precisely adjusted in position alone, so that the adjustment of the relative position relationship between the flow chamber 200 and other components may be completed only by simplified adjustment steps, and thus the adjustment efficiency of the flow chamber assembly 10 may be improved. The positioning surface 110 may position other components in a manner of setting a positioning hole and a positioning bolt, a magnetic attraction positioning manner, and the like, as long as the positioning surface 110 can be matched with the positioning surface to realize a relative positioning relationship.

Referring to fig. 2 to 7, the connector 100 is provided in a plate shape, and the positioning surface 110 is a main surface of the connector 100. The connection member 100 may have a prismatic plate shape, a cylindrical plate shape, etc., and the main surface is one or both bottom surfaces of the plate-shaped connection member 100.

The connection member 100 is disposed to at least partially overlap the detection flow channel 210 in the axial direction of the detection flow channel 210. When the connection member 100 at least partially overlaps the detection flow channel 210, it may be convenient for the light source assembly 20 to be able to irradiate the generated incident light to the detection flow channel 210, and/or it may also be convenient for the backlight assembly 30 to receive the emergent light transmitted from the inside of the detection flow channel 210 to the outside of the flow chamber 200.

Wherein the outer peripheral wall of the flow chamber 200 is provided with a first observation region 310 and a second observation region 320 at least partially overlapping the detection flow channel 210 in the axial direction of the detection flow channel 210, the first observation region 310 is used for allowing incident light to be transmitted from the outside of the flow chamber 200 to the inside of the detection flow channel 210, the second observation region 320 is used for allowing lateral outgoing light formed by the incident light after acting on sample particles to be transmitted from the inside of the detection flow channel 210 to the outside of the flow chamber 200, and the connection member 100 is provided so as to avoid the first observation region 310 and the second observation region 320. The first observation area 310 and the second observation area 320 may be observation windows disposed on the flow chamber 200 or the connection member 100, at least a portion of the detection flow channel 210 may be exposed through both the first observation area 310 and the second observation area 320, and the connection member 100 is disposed to avoid the first observation area 310 and the second observation area 320, so that the first observation area 310 and the second observation area 320 are not blocked by the connection member 100 due to the disposition of the connection member 100, thereby facilitating the reception of incident light by the detection channel or facilitating the transmission of emergent light from the inside of the detection channel to the outside of the flow chamber 200. In this embodiment, the positions of the first observation region 310 and the second observation region 320 are not limited, that is, the first observation region 310 may be configured to receive incident light, and emergent light may be transmitted to the outside of the flow chamber 200 through the second observation region 320; or the second observation region 320 is used to receive incident light, and the emergent light can be transmitted to the outside of the flow chamber 200 through the first observation region 310.

With the above-described embodiment, the flow cell assembly 10 can be positioned with other components by the positioning surface 110, and can also perform optical detection on sample particles through the first observation region 310 and the second observation region 320, so that the installation accuracy can be improved, and the detection of a sample to be detected can be facilitated.

In an embodiment, referring to fig. 2 to 4, the connection element 100 is connected to one side of the flow chamber 200, and one of the first observation region 310 and the second observation region 320 is disposed on the other side of the flow chamber 200 opposite to the connection element 100 along the radial direction of the detection flow channel 210. In this embodiment, the connection member 100 is only disposed on one side of the outer wall of the flow chamber 200, and the other side of the outer wall of the flow chamber 200 can be exposed, at this time, one of the first observation region 310 and the second observation region 320 is disposed on the other side of the flow chamber 200 opposite to the connection member 100 along the radial direction of the detection flow channel 210, that is, the first observation region 310 or the second observation region 320 is disposed on one side of the flow chamber 200 where the connection member 100 is not disposed, one of the first observation region 310 or the second observation region 320 is used for receiving incident light to the detection flow, and the other one is used for transmitting lateral emergent light.

In another embodiment, referring to fig. 5-7, at least a portion of the connector 100 is located on the same side of the flow chamber 200 as one of the first viewing zone 310 and the second viewing zone 320, the flow chamber 200 has an avoidance region 400 that is formed to avoid at least a portion of the connector 100 along the axial direction of the detection flow channel 210, and one of the first viewing zone 310 and the second viewing zone 320 is disposed in the avoidance region 400. The connecting member 100 is located on both sides of the flow chamber 200, wherein the connecting member 100 on one side may be disposed to at least partially overlap the detection flow channel 210 in the axial direction of the detection flow channel 210, and the connecting member 100 on the other side may have a height equal to or slightly lower than that of the detection channel, such that the top of the connecting member 100 to the top of the detection channel forms an avoidance region 400, and the first observation region 310 or the second observation region 320 is located in the avoidance region 400.

Referring to fig. 2-7, in an embodiment, the other of the first and second viewing zones 310, 320 is disposed parallel to the direction of extension of the connector 100. That is, one of the first observation region 310 and the second observation region 320 is located at a side of the flow chamber 200, and the other is disposed parallel to the extending direction of the connection member 100, and the observation region disposed parallel to the extending direction of the connection member 100 can be used for receiving incident light, and the other is used for receiving lateral output light of the flow chamber 200.

The first observation region 310 is disposed parallel to the extending direction of the connection member 100, the peripheral wall of the flow chamber 200 is further provided with a third observation region 330 disposed opposite to the first observation region 310, and the third observation region 330 is used for allowing backward outgoing light formed by the incident light after acting on the sample particles to be transmitted from the inside of the detection flow channel 210 to the outside of the flow chamber 200. The first observation region 310 and the third observation region 330 are located at two opposite sides of the flow chamber 200, the second observation region 320 is located at the side of the flow chamber 200, the first observation region 310 may be open to the light source assembly 20, the second observation region 320 may be open to the side assembly 40, and the third observation region 330 may be open to the backlight assembly 30. The light source assembly 20 emits incident light, the incident light enters the detection flow channel 210 through the first observation region 310 and irradiates the sample particles entering the detection flow channel 210, the sample particles reflect the incident light into linear emergent light and lateral emergent light, the linear emergent light exits to the rear light assembly 30 through the third observation region 330 and is received by the rear light assembly 30, and the lateral emergent light exits to the lateral assembly 40 through the second observation region 320 and is received by the lateral assembly 40.

With the above embodiment, the flow chamber assembly 10 can be positioned with other components by the positioning surface 110, and can perform optical detection on sample particles through the first observation area 310 and the second observation area 320, so that the installation accuracy can be improved, and the detection on a sample to be detected can be facilitated.

In one embodiment, flow chamber 200 and connector 100 are integrally formed, and the optical wall thickness of flow chamber 200 in first and second viewing zones 310 and 320 is between 0.5mm and 4 mm. The flow chamber 200 and the connecting member 100 are integrally formed, so that the assembly between the flow chamber assembly 10 and the sample analyzer is simpler, meanwhile, the integrated flow chamber 200 and the connecting member 100 are not required to be assembled in a bonding mode and the like, and the phenomenon that the relative position of the connecting member 100 and the flow chamber 200 is deviated due to the assembling operation of the connecting member 100 and the flow chamber 200, and finally, the detection error occurs during the detection of the sample analyzer is avoided. The optical wall thickness of the flow cell 200 at the detection channel is between 0.5mm and 4.0 mm. Specifically, the dimensions of the optical wall thickness of the detection flow channel 210 may include: 0.5mm, 1mm, 2mm, 3mm or 4mm, preferably the optical wall thickness at the detection channel may be between 1mm-2.0 mm. In this embodiment, the optical wall thickness may be interpreted as the perpendicular distance of the outer wall of the detection channel to the central axis of the detection channel.

The principle and the implementation of the present application are explained herein by applying specific examples, and the above description of the embodiments is only used to help understand the method and the core idea of the present application; meanwhile, for a person skilled in the art, according to the idea of the present application, the specific implementation manner and the application scope may be changed, and in summary, the content of the present specification should not be construed as a limitation to the present application.

Claims (10)

1. A flow chamber assembly comprising a flow chamber provided with a detection flow channel for receiving a sheath flow containing sample particles and moving the sample particles in the sheath flow in an axial direction of the detection flow channel, and a connecting member connected to an outer peripheral wall of the flow chamber and extending outward of the flow chamber in a radial direction of the detection flow channel, the connecting member having a positioning surface parallel to the axial direction of the detection flow channel for positioning the flow chamber assembly when the flow chamber assembly is mounted;

wherein the peripheral wall of the flow chamber is provided with a first observation area and a second observation area at least partially overlapping with the detection flow channel in the axial direction of the detection flow channel, the first observation area is used for allowing incident light to be transmitted from the outside of the flow chamber to the inside of the detection flow channel, the second observation area is used for allowing lateral emergent light formed by the incident light after acting on the sample particles to be transmitted from the inside of the detection flow channel to the outside of the flow chamber, and the connecting piece is arranged to avoid the first observation area and the second observation area.

2. The flow chamber assembly of claim 1, wherein the connector is connected to one side of the flow chamber, and one of the first and second viewing zones is disposed on the other side of the flow chamber opposite the connector in a radial direction of the detection flow channel.

3. The flow chamber assembly of claim 1 wherein at least a portion of the connector is on the same side of the flow chamber as one of the first and second viewing zones, the flow chamber having an avoidance region forming an avoidance with at least a portion of the connector in an axial direction of the inspection flow path, one of the first and second viewing zones being disposed within the avoidance region.

4. The flow chamber assembly of claim 2 or 3, wherein the other of the first and second viewing zones is disposed parallel to the direction of extension of the connector.

5. The flow chamber assembly according to claim 4, wherein the first observation area is provided in parallel with an extending direction of the connecting member, and the peripheral wall of the flow chamber is further provided with a third observation area provided opposite to the first observation area, the third observation area being configured to allow backward-emerging light formed after the incident light has acted on the sample particles to be transmitted from the inside of the detection flow channel to the outside of the flow chamber.

6. A flow chamber assembly according to any of claims 1-3, wherein the connecting member is arranged to at least partially overlap the test flow channel in an axial direction of the test flow channel.

7. A flow chamber assembly according to any of claims 1-3, characterised in that the connecting member is plate-like in configuration, and the positioning surface is a main surface of the connecting member.

8. The flow cell assembly of any of claims 1-3, wherein the flow cell and the connector are integrally formed, and wherein the flow cell has an optical wall thickness in the first and second viewing zones of between 0.5mm and 4 mm.

9. An optical inspection apparatus comprising a flow chamber assembly of any of claims 1-8, an inspection assembly, and a light source assembly for emitting incident light to the inspection flow path; the detection assembly is used for receiving emergent light formed after the incident light acts on the sample particles.

10. A sample analyzer, comprising: a sampling module for obtaining a sample, a sample preparation module for receiving the sample and mixing the sample with a reagent to obtain a sample to be tested, and the flow chamber assembly of any of claims 1-8.

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