CN110208519A - A kind of active liquid stream control micro-fluidic detection system - Google Patents
A kind of active liquid stream control micro-fluidic detection system Download PDFInfo
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- CN110208519A CN110208519A CN201910566908.1A CN201910566908A CN110208519A CN 110208519 A CN110208519 A CN 110208519A CN 201910566908 A CN201910566908 A CN 201910566908A CN 110208519 A CN110208519 A CN 110208519A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
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- Hematology (AREA)
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- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention is suitable for micro-fluidic chip electrochemiluminescent immunoassay detection technique field, a kind of active liquid stream control micro-fluidic detection system is provided, the system comprises active liquid stream control micro fluidic devices and the active liquid stream being placed on active liquid stream control micro fluidic device to control micro-fluidic chip；The active liquid stream control micro fluidic device includes: pedestal；It is placed in pedestal and the accommodation groove for placing active liquid stream control micro-fluidic chip；For collecting or dispersing the power mechanism of the magnetic bead mix on active liquid stream control micro-fluidic chip；The power mechanism includes: magnet；Force piece；Shaking device.Accommodation groove is arranged by being located on pedestal in the present invention, and active liquid stream control micro-fluidic chip is placed on accommodation groove, magnetic bead is collected by magnet again, disperse magnetic bead by shaking device, by force piece moving magnet, so that the mobile magnetic bead collected by magnet, can guide the collection or dispersion of the magnetic bead mix on active liquid stream control micro-fluidic chip, and the movement of magnetic bead mix is guided, the accuracy of detection can be effectively improved.
Description
Technical field
The invention belongs to micro-fluidic chip electrochemiluminescent immunoassay detection technique fields more particularly to a kind of active liquid stream to control miniflow
Control detection system.
Background technique
Currently, there are mainly two types of development trends for in-vitro diagnosis (IVD): one is automations, integrated integration, i.e., using big
Full-automatic, the highly sensitive large-scale instrument and equipment of matched central laboratory, type hospital realizes that high-precision diseases analysis is examined
Disconnected, the reagent of use is big packaging reagent, for multiple sample analysis；Another kind miniaturization, bed sideization analyzer, using list
Person-portion packs reagent, realizes the quick analyzing and diagnosing in scene.
Small hospital or community hospital's insufficient funds, sample size are few, are not appropriate for the large scale equipment of purchasing price valuableness, and
The sample for needing to analyze is few, and packs greatly after reagent is sealed off using limited time, leads to the expired waste of reagent.And point minimized
Analyzer can solve that small hospital or community hospital's large scale equipment are at high cost, reagent waste ask using single packaged reagent
Topic.
Micro-fluidic chip is also known as chip lab (Lab-on-a-chip), refers to fields such as biology, chemistry and medicine
Involved in the basic operation units such as sample preparation, reaction, separation, detection be integrated into one piece with micro-meter scale microchannel
On chip, it is automatically performed the overall process of reaction and analysis.Based on micro-fluidic chip realize analysis and detection device the advantages of be: sample
This dosage is few, and analysis speed is fast, convenient for portable instrument is made, is highly suitable for instant, field assay.
But existing single packaged micro-fluidic chip is when detecting, passive liquid stream coutroi velocity is uncontrollable, and flow direction is not
It can invert, influence the accuracy examined.
Summary of the invention
The embodiment of the present invention provides a kind of active liquid stream control micro-fluidic detection system, it is intended to solve existing micro-fluidic chip
When detecting, passive liquid stream coutroi velocity is uncontrollable, and flow direction cannot invert, and influences the accuracy examined.
The embodiments of the present invention are implemented as follows, provides a kind of active liquid stream control micro-fluidic detection system, the system
The active liquid stream for controlling micro fluidic device including active liquid stream and being placed on active liquid stream control micro fluidic device controls miniflow
Control chip；The active liquid stream controls micro fluidic device
Pedestal；
It is set to pedestal and the accommodation groove for placing active liquid stream control micro-fluidic chip；
For collecting or dispersing the power mechanism of the magnetic bead mix on active liquid stream control micro-fluidic chip；
The power mechanism includes:
For collecting the magnet of the magnetic bead mix of active liquid stream control micro-fluidic chip；
The mobile force piece of drive magnet；
For dispersing the shaking device of the magnetic bead mix of active liquid stream control micro-fluidic chip.
Further, the active liquid stream control micro-fluidic chip includes:
Substrate；
Be arranged on the substrate, for sample be added and store sample-adding portion；
Be arranged the first storage unit on the substrate and the marked by magnetic bead ligand being stored in first storage unit and
Luminescent marking ligand；
Mixing and reacting with the marked by magnetic bead ligand, luminescent marking ligand for the sample on the substrate is set
Generate the reaction zone of magnetic bead mix；
The cleaning solution that the second storage unit on the substrate is set and is stored in second storage unit；
Detection zone on the substrate is set；
Between the reaction zone and the sample-adding portion, between the reaction zone and first storage unit, the reaction zone
Interconnecting piece is provided between second storage unit, between the detection zone and the reaction zone.
Further, first storage unit includes:
Store the first storage chamber of the marked by magnetic bead ligand；
Store the second storage chamber of the luminescent marking ligand.
Further, the marked by magnetic bead ligand is the homogeneous magnetic standard configuration body of liquid, the homogeneous magnetic standard configuration body packet of liquid
Include magnetic bead, temperature-sensitive material and solution.
Further, the chip further include:
The third storage unit being arranged on institute's substrate and the luminescent solution being stored in the third storage unit；
Interconnecting piece is provided between the third storage unit and the detection zone.
Further, cleaning area is additionally provided between the detection zone and the reaction zone.
Further, the sample-adding portion, the first storage unit, the second storage unit, third storage unit, reaction zone, detection zone
And in each interconnecting piece, micro- channel structure is used at least one, it is micro-meter scale that micro- channel is at least one-dimensional.
Advantageous effects of the invention: accommodation groove is arranged by being located on pedestal in the present invention, and by active liquid stream control
Micro-fluidic chip processed is placed on accommodation groove, then collects magnetic bead by magnet, is dispersed magnetic bead by shaking device, is passed through force piece
Moving magnet, so that the mobile magnetic bead collected by magnet, can guide the magnetic bead mixing on active liquid stream control micro-fluidic chip
The collection or dispersion of object, and the movement of magnetic bead mix is guided, the accuracy of detection can be effectively improved.
Detailed description of the invention
Fig. 1 is the perspective view of active liquid stream control micro-fluidic detection system one embodiment provided in an embodiment of the present invention.
Fig. 2 is the perspective view of active liquid stream control micro-fluidic chip one embodiment provided in an embodiment of the present invention.
Fig. 3 is the step process of the reaction method of active liquid stream control micro-fluidic detection system provided in an embodiment of the present invention
Figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
The present invention controls micro fluidic device by using setting active liquid stream, sets on active liquid stream control micro fluidic device
Power mechanism is set, by the effect of power mechanism, the movement of magnetic bead mix can be guided, the accurate of detection can be effectively improved
Property.
Embodiment one
As shown in FIG. 1 to 3, the embodiments of the present invention are implemented as follows, provides a kind of active liquid stream control micro-fluidic inspection
Examining system, the system comprises active liquid stream control micro fluidic device and the masters being placed on active liquid stream control micro fluidic device
Hydrodynamic flow control micro-fluidic chip；The active liquid stream controls micro fluidic device
Pedestal 5；
It is set to pedestal 5 and the accommodation groove 51 for placing active liquid stream control micro-fluidic chip；
For collecting or dispersing the power mechanism of the magnetic bead mix on active liquid stream control micro-fluidic chip；
The power mechanism includes:
For collecting the magnet 52 of the magnetic bead mix of active liquid stream control micro-fluidic chip；
The mobile force piece 31 of drive magnet 52；
For dispersing the shaking device 41 of the magnetic bead mix of active liquid stream control micro-fluidic chip.
Specifically, the force piece 31 is linear motor；Shaking device 41 is vibration of ultrasonic wave device.Linear motor can be simultaneously
Control the movement of magnet 52 and shaking device 41.
In the present embodiment, magnetic bead can be collected together by magnet 52, and pass through 31 moving magnet 52 of force piece, energy
It, being capable of magnetic bead enough so that 52 displacement of magnet, so that magnetic bead follows displacement, then passes through the effect of shaking device 41
Vibration dispersion, meanwhile, it is uniformly mixed convenient for the intracorporal magnetic bead of magnetic bead standard configuration, is conducive to subsequent detection reaction, master can be guided
The collection or dispersion of magnetic bead mix on hydrodynamic flow control micro-fluidic chip, so that the movement of magnetic bead mix is guided, it can
Effectively improve the accuracy of detection.
Accommodation groove 51 is arranged by being located on pedestal 5 in the present invention, and active liquid stream control micro-fluidic chip is placed in appearance
It sets on slot 51, then magnetic bead is collected by magnet 52, magnetic bead is dispersed by shaking device 41, by 31 moving magnet 52 of force piece,
To the mobile magnetic bead collected by magnet 52, the collection of the magnetic bead mix on active liquid stream control micro-fluidic chip can be guided
Or dispersion, and the movement of magnetic bead mix is guided, the accuracy of detection can be effectively improved.
Embodiment two
The active liquid stream controls micro-fluidic chip
Substrate 11；
Sample-adding portion 21 on the substrate 11, being added and store for sample is set；
The first storage unit being arranged on the substrate 11 and the marked by magnetic bead ligand being stored in first storage unit
And luminescent marking ligand；
Mixing for the sample with the marked by magnetic bead ligand, luminescent marking ligand on the substrate 11 and anti-is set
The reaction zone 24 of magnetic bead mix should be generated；
The second storage unit 25 being arranged on the substrate 11 and the cleaning solution being stored in second storage unit 25；
Detection zone 28 on the substrate 11 is set；
Between the reaction zone and the sample-adding portion 21, between the reaction zone and first storage unit, described react
Interconnecting piece 27 is provided between area and second storage unit 25, between the detection zone 28 and the reaction zone.
Storage marked by magnetic bead ligand and hair is arranged by using marked by magnetic bead ligand and luminescent marking ligand in the present embodiment
First storage unit of signal ligand enables marked by magnetic bead ligand and luminescent marking ligand stable storage in the first storage unit
It is interior, to improve the sensitivity accuracy of detection.
Specifically, the cleaning solution removes analyte, the luminous agent marker of non-specific adsorption for cleaning magnetic bead
And the substance of other influences testing result.Cleaning solution mainly includes buffer system, protein and surfactant, wherein buffer body
System is 6.0~10.0 including but not limited to borate, phosphate, Tris-HCl and acetate etc., the pH range of cleaning solution.Wherein
Protein is including but not limited to bovine serum albumin(BSA), casein etc..Wherein surface-active including but not limited to may include polysorbas20,
Tween 80, triton x-100, polyethylene glycol and polyvinylpyrrolidone etc..Preferably, using cleaning in the present embodiment
Liquid is the pH7.0Tris-HCl buffer comprising bovine serum albumin(BSA), tween 20 and Proclin300.
Specifically, first storage unit, the second storage unit 25 are seal chamber, sealing material used using elastic material or
High-isolation film, specially glass, plastics, rubber, aluminium foil or high-isolation film, wherein sealing material can be same material group
At can also be composed for multiple material.Under physical impact, the first storage unit, the second storage unit 25 can partial fracture, thus
The material of storage is released.
Specifically, identical or different material and method production can be used in first storage unit and the second storage unit 25.It is excellent
Choosing, the first storage unit and the second storage unit 25 is all made of plastics and elastic rubber is sealed to form.Another is preferred, and first deposits
Storage portion is sealed to form using plastics and elastic rubber, and the second storage unit 25 is sealed to form using high-isolation film.
Specifically, in order to seal chip interior, sealing cover is additionally provided at the adding mouth in sample-adding portion 21, sample is added
After in sample-adding portion 21, then cover sealing cover.
Specifically, the luminescent marking ligand includes the ligand of enzyme label.
Enzyme is in conjunction with analyte or competes, and forms enzyme tagged ligand；Magnetic particle marker is in conjunction with analyte or competes, and is formed
Marked by magnetic bead ligand, both described ligands may be the same or different；The ligand that the magnetic enzyme tagged ligand, enzyme tagged ligand use
Comprising nucleic acid, antigen, monoclonal antibody, polyclonal antibody and hormone receptor, the analyte include DNA, small molecule (drug or
Drugs), antigen, antibody, hormone, antibiotic, bacterium or virus and other biochemical markers.
The enzyme tagged ligand uses chemiluminescence method.The enzyme includes: in horseradish peroxidase and alkaline phosphatase
It is one or more.Specifically, the ligand includes: one of antigen, antibody, haptens and nucleic acid or a variety of；The hair
Signal ligand can compete (such as competition law) with marked by magnetic bead ligand binding (such as double antibody sandwich method) or with tagged ligand.Its
The ligand of middle enzyme label can be identical as marked by magnetic bead ligand, can also be different.Preferably, in one embodiment of the present of invention
In, select two kinds of different antibodies to test and analyze object as luminescent marking ligand and marked by magnetic bead ligand with double antibody sandwich method.This
In another embodiment of invention, a kind of antigen and a kind of antibody are selected, is matched respectively as luminescent marking ligand and marked by magnetic bead
Body detects sample with competition law.
Embodiment three
On the basis of example 1, the liquid tagged ligand includes the ligand of luminous agent label.The luminous agent mark
Remember that ligand uses physics luminescent method, i.e. fluorescence radiation.
The luminous agent can also include: one of acridinium ester, ABEI, fluorescent dye, fluorescin and fluorescent microsphere
Or it is a variety of.
The cleaning solution storage unit 25 and the cleaning solution being stored in cleaning solution storage unit 25；
The detection zone 28 being connected to the cleaning area 29；
Luminescent solution storage unit 26 and the luminescent solution being stored in luminescent solution storage unit 26, for further cleaning magnetic bead or increasing
Strong luminous signal；
Between the cleaning area 29 and the detection zone 28, between the luminescent solution storage unit 26 and the detection zone 28
It is provided with interconnecting piece 27.
Specifically, the ligand includes: one of antigen, antibody, haptens and nucleic acid or a variety of.
Luminous agent is in conjunction with analyte or competes, and forms luminous agent tagged ligand；Magnetic particle marker in conjunction with analyte or
Competition, forms marked by magnetic bead ligand, both described ligands may be the same or different；The magnetic marker ligand, luminous agent label are matched
The ligand that body uses include nucleic acid, antigen, monoclonal antibody, polyclonal antibody and hormone receptor, the analyte include DNA,
Small molecule (drug or drugs), antigen, antibody, hormone, antibiotic, bacterium or virus and other biochemical markers.
In the present embodiment, the liquid tagged ligand can with the homogeneous magnetic mark ligand binding (such as double antibody sandwich method) of liquid or
Person and tagged ligand competition (such as competition law).Wherein the ligand of luminous agent label can be identical as the homogeneous magnetic standard configuration body of liquid,
It can be different.Preferably, in one embodiment of the invention, selecting two kinds of different antibodies as liquid tagged ligand and liquid
The homogeneous magnetic standard configuration body of state tests and analyzes object with double antibody sandwich method.
Example IV
First storage unit includes:
Store the first storage chamber 231 of the marked by magnetic bead ligand；
Store the second storage chamber 232 of the luminescent marking ligand.
By the present invention in that with marked by magnetic bead ligand and luminescent marking ligand, and be arranged and deposit the first storage chamber 231 and second
Storage chamber 232, makes marked by magnetic bead ligand and luminescent marking ligand is stored in respective storage unit when chip is not used, when detection
It is sufficiently mixed marked by magnetic bead ligand or luminescent marking ligand with sample is added, the speed of reaction can be effectively improved,
To improve sensitivity and the accuracy of detection.
Embodiment five
The marked by magnetic bead ligand is the homogeneous magnetic standard configuration body of liquid, and the homogeneous magnetic standard configuration body of liquid includes magnetic bead, temperature
Sensitive material and solution.Wherein, magnetic bead includes one of Streptavidin MagneSphere, antibody modification magnetic bead and Modified antigen magnetic bead
Or it is a variety of；Temperature-sensitive material is heat-convertible gel, comprising gelatin, agar, alginate, carragheen, hydroxymethyl cellulose, Ah
Draw one of primary glue, guar gum, the ashamed bean gum of thorn, pectin, starch and xanthan gum or a variety of；Solution is to contain surface-active
Agent, protein buffer system.Due to the good fluidity of liquid, when detection, make the homogeneous magnetic standard configuration body of liquid, enzyme tagged ligand with
The sample three of addition is sufficiently mixed, and can effectively improve the speed of reaction, to improve the sensitivity of detection, repeatability
And accuracy.
Embodiment six
The chip further include:
The third storage unit 26 being arranged on institute's substrate 11 and the luminescent solution being stored in the third storage unit 26；
Interconnecting piece 27 is provided between the third storage unit 26 and the detection zone 28.
In the present embodiment, the enzyme uses one of horseradish peroxidase and alkaline phosphatase or a variety of；It is described to shine
Agent uses one of acridinium ester and ABEI or a variety of；It carries out chemiluminescence and needs luminescent solution, and then needed using fluorescence radiation
Light emitting source.
Detection zone 28 is set, convenient for resulting composite being moved to detection zone 28 and is seen after cleaning solution being cleaned
It examines and detects, be easy to use, it is simple and convenient.
Embodiment seven
Cleaning area 29 is additionally provided between the detection zone 28 and the reaction zone.Specifically, the substrate 11 is additionally provided with
The waste liquid storage unit 30 being connected to the cleaning area 29.Convenient for collecting the waste liquid after cleaning and reaction, waste liquid can reduce to inspection
The interference of survey effectively improves the precision of detection.
Embodiment eight
The sample-adding portion 21, the first storage unit, the second storage unit 25, third storage unit 26, reaction zone 24, detection zone 28 with
And in each interconnecting piece 27, micro- channel structure is used at least one, it is micro-meter scale that micro- channel is at least one-dimensional.
Micro-fluidic chip of the invention will test all reagent component (luminescent marking ligands, marked by magnetic bead needed for process
Ligand, cleaning solution, luminescent solution etc.) it integrates, be built into micro-fluidic chip, and by ingenious micro- channel design, in mating instrument
Under the operation of device, the operating in a key (only need to can be achieved with detecting by start button, be not necessarily to complex operations) of micro-fluidic chip is realized,
It realizes whole blood separation, immune response, cleaning separation, chemiluminescence detection, is set so as to avoid structure in existing micro-fluidic chip
Meter is simple, detection when it is complicated for operation the deficiencies of and defect.Serum or blood plasma inspection can only be carried out by also overcoming traditional chemical light-emitting appearance
The shortcomings that surveying, and whole blood sample cannot being detected.
Embodiment nine
The reaction method of active liquid stream provided by the invention control micro-fluidic detection system includes:
By in sample, the first storage unit marked by magnetic bead ligand and enzyme tagged ligand or Fluorescein labeled ligand be put into reaction
Area；
Match the mixture of the sample and sample liquid with the marked by magnetic bead ligand, enzyme label by power mechanism
Body or Fluorescein labeled ligand are sufficiently mixed in reaction zone, react；
Compound after cleaning reaction.
Fluorescein labeled ligand, that is, luminous agent above-mentioned label ligand.
In the present embodiment, matched by the mixture that power mechanism is able to drive sample and sample liquid with the marked by magnetic bead
Body, the enzyme tagged ligand or Fluorescein labeled ligand are sufficiently mixed in reaction zone, react, and can effectively improve the accurate of detection
Property.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (7)
1. a kind of active liquid stream controls micro-fluidic detection system, which is characterized in that the system comprises active liquid streams to control miniflow
Control device and the active liquid stream control micro-fluidic chip being placed on active liquid stream control micro fluidic device；The active liquid stream control
Micro fluidic device processed includes:
Pedestal；
It is set to pedestal and the accommodation groove for placing active liquid stream control micro-fluidic chip；
For collecting or dispersing the power mechanism of the magnetic bead mix on active liquid stream control micro-fluidic chip；
The power mechanism includes:
For collecting the magnet of the magnetic bead mix of active liquid stream control micro-fluidic chip；
The mobile force piece of drive magnet；
For dispersing the shaking device of the magnetic bead mix of active liquid stream control micro-fluidic chip.
2. active liquid stream as described in claim 1 controls micro-fluidic detection system, which is characterized in that the active liquid stream control
Micro-fluidic chip includes:
Substrate；
Be arranged on the substrate, for sample be added and store sample-adding portion；
The first storage unit on the substrate and the marked by magnetic bead ligand being stored in first storage unit are set and shone
Tagged ligand；
Mixing for the sample with the marked by magnetic bead ligand, luminescent marking ligand and reacting generation on the substrate is set
The reaction zone of magnetic bead mix；
The cleaning solution that the second storage unit on the substrate is set and is stored in second storage unit；
Detection zone on the substrate is set；
Between the reaction zone and the sample-adding portion, between the reaction zone and first storage unit, the reaction zone and institute
It states between the second storage unit, be provided with interconnecting piece between the detection zone and the reaction zone.
3. active liquid stream as claimed in claim 2 controls micro-fluidic detection system, which is characterized in that the first storage unit packet
It includes:
Store the first storage chamber of the marked by magnetic bead ligand；
Store the second storage chamber of the luminescent marking ligand.
4. as the described in any item active liquid streams of Claims 2 or 3 control micro-fluidic detection system, which is characterized in that the magnetic
Pearl tagged ligand is the homogeneous magnetic standard configuration body of liquid, and the homogeneous magnetic standard configuration body of liquid includes magnetic bead, temperature-sensitive material and solution.
5. active liquid stream as claimed in claim 2 controls micro-fluidic detection system, which is characterized in that the chip further include:
The third storage unit being arranged on institute's substrate and the luminescent solution being stored in the third storage unit；
Interconnecting piece is provided between the third storage unit and the detection zone.
6. active liquid stream as claimed in claim 5 controls micro-fluidic detection system, which is characterized in that the detection zone with it is described
Cleaning area is additionally provided between reaction zone.
7. as the described in any item active liquid streams of claim 5 or 6 control micro-fluidic detection system, which is characterized in that described to add
In sample portion, the first storage unit, the second storage unit, third storage unit, reaction zone, detection zone and each interconnecting piece, at least one
Using micro- channel structure, it is micro-meter scale that micro- channel is at least one-dimensional.
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