CN215250859U - Automatic liquid-separating and adding device - Google Patents
Automatic liquid-separating and adding device Download PDFInfo
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- CN215250859U CN215250859U CN202120802347.3U CN202120802347U CN215250859U CN 215250859 U CN215250859 U CN 215250859U CN 202120802347 U CN202120802347 U CN 202120802347U CN 215250859 U CN215250859 U CN 215250859U
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Abstract
The utility model belongs to the technical field of the sample inspection equipment, concretely relates to automatic divide liquid to add device. The utility model provides an automatic liquid-separating and adding device, which comprises a driving platform, a liquid-transferring gun, a reagent placing area, a sample placing area and a horizontal table top; the driving platform, the pipette, the reagent placing area, the sample placing area and the horizontal table top are arranged on the horizontal table top. The device is applied to a fluorescent quantitative PCR (polymerase chain reaction) inspection technology, the reagent is added into a sample container from a reagent bottle in a timed and quantitative manner in a full-automatic manner, the next PCR inspection is conveniently carried out, the device controls the driving platform to move in three directions through the control system, so that the liquid-transferring gun can move back and forth to each functional area in a full-automatic manner without manual operation, and the working efficiency is greatly improved; meanwhile, the control system is used for controlling the reagent amount absorbed and released by the liquid-transfering gun, the added actual amount is accurately controlled, and the sample amount is prevented from deviating and affecting the final test result.
Description
Technical Field
The utility model belongs to the technical field of the sample inspection equipment, concretely relates to automatic divide liquid to add device.
Background
The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of the PCR is that trace amount of DNA can be greatly increased. Therefore, even if only a single spot of DNA can be isolated from the hairs, skin or blood left by the murder in the murder case, or the remains of the historic creatures and the historical characters in the fossil, the DNA can be amplified by PCR and compared. This is also the strength of the "micro evidence". The currently common PCR detection technology is a fluorescence quantitative PCR detection technology, which is a method for adding a fluorescent group into a PCR reaction system, monitoring the whole PCR process in real time by using fluorescence signal accumulation, and finally carrying out quantitative analysis on an unknown template through a standard curve.
The existing PCR detection process also widely depends on manual sampling and liquid separation of experimenters, so that the working efficiency is low, repeated work is large in physical and mental tests on the experimenters, sampling errors are easy to occur, quantification and timing cannot be accurately guaranteed, and the experimental result cannot be accurately guaranteed.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides an automatic liquid-separating and adding device, which comprises a driving platform, a liquid-transferring gun, a reagent placing area, a sample placing area and a horizontal table board; drive platform, pipetting gun, reagent place the district, the sample place the district, set up in on the horizontal table surface, the pipetting gun sets up drive platform is last, drive platform realizes the pipetting gun is followed the reagent is placed the district and is arrived the reciprocating motion in sample place the district.
As a preferable technical solution, the driving platform includes a first direction shaft and a second direction shaft, the first direction shaft and the second direction shaft are perpendicular to each other, the second direction shaft is disposed on the first direction shaft, and the moving directions of the first direction shaft and the second direction shaft are both parallel to the horizontal table top.
As a preferable technical solution, the driving platform further includes a third direction shaft, the third direction shaft is disposed at an end position of the second direction shaft, and a moving direction of the third direction shaft is perpendicular to the horizontal table top.
As a preferred technical scheme, the device further comprises a lance head placing area, wherein the lance head placing area is arranged on the horizontal table top, and the lance head placing area and the reagent placing area are arranged adjacently.
As a preferable technical scheme, the driving platform realizes the sequential reciprocating movement of the pipette from the pipette head placing area, through the reagent placing area and to the sample placing area.
As a preferred technical scheme, the device also comprises a gun head recovery box, and the gun head recovery box is arranged on the horizontal table board.
As a preferred technical scheme, the driving platform realizes sequential reciprocating movement of the pipette from the pipette head placing area, through the reagent placing area, through the sample placing area and to the pipette head recovery box.
As a preferred technical scheme, the liquid-transfering gun comprises a motor and a piston, wherein the motor is connected with the piston through a transmission device.
As a preferable technical scheme, the liquid-transferring device further comprises a control mechanism, wherein a control system is arranged in the control mechanism and is connected with the driving platform and the liquid-transferring gun; the control mechanism is a computer touch screen.
As a preferred technical scheme, the horizontal table top is provided with a mounting assembly fixedly connected with the driving platform, the liquid-transferring gun, the reagent placing area, the sample placing area, the gun head placing area and the gun head recovery box.
Has the advantages that:
(1) the utility model provides an automatic liquid-separating and adding device, which is applied to the fluorescent quantitative PCR inspection technology, realizes that reagent is added into a sample container from a reagent bottle in a timed and quantitative manner in a full-automatic manner, and is convenient for the next PCR inspection; meanwhile, the control system is used for controlling the reagent amount absorbed and released by the liquid-transfering gun, the added actual amount is accurately controlled, and the sample amount is prevented from deviating and affecting the final test result.
(2) The positions of the functional areas are reasonably planned, the gun head placing area and the reagent placing area are arranged adjacently, so that the distance from the gun head placing area to the sample placing area through the reagent placing area is the shortest, the equipment operation time is saved, and the efficiency is further improved.
(3) The horizontal table top is provided with the installation component fixedly connected with each functional area, so that on one hand, the identification time of workers during installation and disassembly of the device is saved, and installation errors are avoided, on the other hand, the fixed positions are set for each functional area, the calibration of an application program is facilitated, and errors in the use process are avoided; meanwhile, the situation that the liquid gun cannot accurately reach the designated position under the established program due to the fact that each functional area moves is avoided.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural view of an automatic liquid-separating and adding device provided by the present invention;
FIG. 2 is a schematic structural diagram of a driving platform;
FIG. 3 is a schematic view of the structure of a reagent placement area;
FIG. 4 is a schematic view of the structure of a sample-placing region;
FIG. 5 is a schematic structural view of a lance tip placement area;
FIG. 6 is a schematic structural view of a gun head recovery box and a control mechanism;
the system comprises a driving platform 1, a first direction shaft 11, a second direction shaft 12, a third direction shaft 13, a liquid transferring gun 2, a reagent placing area 3, a sample placing area 4, a horizontal table top 5, a gun head placing area 6, a gun head recycling box 7 and a control mechanism 8.
Detailed Description
The disclosure may be further understood in conjunction with the following detailed description of preferred embodiments of the invention and the examples included.
When describing embodiments of the present application, the use of "preferred," "preferably," "more preferred," etc., refers to embodiments of the invention that may provide certain benefits under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. In addition, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.
In this document, relational terms such as first, second, and the like may be used solely to distinguish one entity from another entity without necessarily requiring or implying any actual such relationship or order between such entities. Furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a component, apparatus, or device that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such component, apparatus, or device.
When a component, element, or layer is referred to as being "on," "bonded to," "connected to," or "coupled to" another element or layer, it may be directly on, bonded to, connected to, or coupled to the other element, or layer, or intervening elements or layers may be present. In contrast, when an element is referred to as being "directly on," "directly coupled to," "directly connected to" or "directly coupled to" another element or layer, there may be no intervening elements or layers present. Other words used to describe the relationship between elements should be interpreted in a similar manner (e.g., "between … …" versus "directly between … …," "adjacent" versus "directly adjacent," etc.). As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The utility model provides an automatic liquid-separating and adding device, which comprises a driving platform, a liquid-transferring gun, a reagent placing area, a sample placing area and a horizontal table top; drive platform, pipetting gun, reagent place the district, the sample place the district, set up in on the horizontal table surface, the pipetting gun sets up drive platform is last, drive platform realizes the pipetting gun is followed the reagent is placed the district and is arrived the reciprocating motion in sample place the district.
The driving platform is used for controlling the position change of the liquid transfer gun, the liquid transfer gun is used for sucking and adding the reagent, the reagent placing area is used for placing the reagent bottles, the number of the reagent bottles is 1-5, and the sample placing area is used for placing the sample to be detected;
preferably, the horizontal table is arranged horizontally with the ground, and particularly, a reagent page placed in a test tube or a reagent bottle on the horizontal table is kept horizontal.
Preferably, the horizontal table-board is marble material for keep drive platform operates steadily, and vibrations are little, avoid reagent to lead to unnecessary wall built-up even to spill over because of vibrations.
In some preferred embodiments, the driving platform includes a first direction axis and a second direction axis, the first direction axis and the second direction axis are perpendicular to each other, the second direction axis is disposed on the first direction axis, and the moving directions of the first direction axis and the second direction axis are both parallel to the horizontal table top.
Preferably, the first direction shaft is responsible for movement and travel in the X direction, a transmission mode of a cable drag chain is adopted, and the first direction shaft comprises an X-axis motor, an X-axis motor movement cable drag chain and an X-axis movement slide rail;
the second direction shaft is responsible for moving and advancing in the Y direction and adopts a transmission mode of a motor slide rail; the second direction shaft comprises a Y-axis motor and a Y-axis moving slide rail.
In some preferred embodiments, the driving platform further comprises a third direction shaft disposed at an end position of the second direction shaft, and a moving direction of the third direction shaft is perpendicular to the horizontal table top.
The third directional shaft is responsible for moving and advancing in the Z direction and adopts a transmission mode of a motor sliding rail; the third direction shaft comprises a Z-axis motor and a Z-axis moving slide rail.
In some preferred embodiments, the reagent storage device further comprises a lance tip placement area, wherein the lance tip placement area is arranged on the horizontal table surface, and the lance tip placement area is arranged adjacent to the reagent storage area;
specifically, the gun head placing area and the reagent placing area are arranged in parallel in a clinging manner.
Preferably, the reagent placing area comprises a base for placing the gun head placing area, so that the reagent bottle and the gun head are arranged in parallel and are in close contact.
In some preferred embodiments, the driving platform realizes the sequential reciprocating movement of the pipette from the pipette tip placing region, through the reagent placing region, and to the sample placing region.
In some preferred embodiments, the lance tip recovery box is arranged on the horizontal platform surface.
Preferably, the gun head recovery box is provided with a first sensor and a second sensor, the first sensor is used for detecting whether the gun head recovery box is full, the gun head recovery box is close to the full box to inform a user of treatment, and the gun head recovery box alarms and stops; the second sensor is used for detecting whether the gun head falls off normally, and if the gun head does not fall off normally, the gun head repeats falling off action and stops the machine to alarm when falling off fails for three times.
The influence of unexpected conditions on the whole process is reduced to the maximum extent, and the reagent in the reagent bottle is damaged due to equipment failure.
In some preferred embodiments, the driving platform realizes the sequential reciprocating movement of the pipette from the pipette tip placing area, through the reagent placing area, through the sample placing area and to the pipette tip recovery box.
In some preferred embodiments, the pipette gun includes a motor and a piston, the motor being connected to the piston through a transmission.
Preferably, the motor is a stepping motor, so that higher operation precision is provided, and the ultrahigh quantitative requirement on the reagent in the reagent adding process is ensured.
In some preferred embodiments, the liquid-transferring device further comprises a control mechanism, wherein a control system is arranged in the control mechanism, and the control system is connected with the driving platform and the liquid-transferring gun; the control mechanism is a computer touch screen.
In some preferred embodiments, the horizontal table top is provided with a mounting assembly fixedly connected with the driving platform, the pipette, the reagent placing area, the sample placing area, the pipette tip placing area and the pipette tip recovery box.
The working principle is as follows:
the utility model provides an automatic liquid-separating adding device, which is applied to the fluorescent quantitative PCR inspection technology, realizes that reagent is added into a sample container from a reagent bottle in a timed and quantitative manner in a full-automatic manner, drives a piston to move through a motor in a liquid-transferring gun, realizes the drawing action, simulates a hand to draw a syringe, and transfers the reagent into a target sample bottle in a sample placing area after the quantitative reagent is drawn out from the reagent placing area; in some preferred embodiments, the pipette under the control of the control system first moves to the tip placement area, clamps a tip, and then moves to a reagent bottle in the reagent placement area to aspirate a quantitative amount of reagent; next, the pipette is moved to the sample placement area to add liquid to the fixed position sample vial or test tube; after the sample is added for the first time, if another reagent needs to be sucked, the pipette tip moves to the tip recovery box and discards the tip, and then the pipette tip returns to the tip placement area to repeat the process; if the same reagent is still added, the pipette returns to the reagent placement area to continue aspirating the reagent, and the next procedure is repeated.
The gun head recovery box is provided with a first sensor and a second sensor, the first sensor is used for detecting whether the gun head recovery box is full, the gun head recovery box is close to the full box to inform a user of treatment, and the gun head recovery box is alarmed and stopped when the gun head recovery box is full; the second sensor is used for detecting whether the gun head falls off normally, and if the gun head does not fall off normally, the gun head repeats falling off action and stops the machine to alarm when falling off fails for three times.
All the processes are completed through the movement of the driving platform and the action of the liquid-transfering gun, and the movement of the driving platform and the action of the liquid-transfering gun are fully automatically controlled by the control system.
Examples
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Without conflict, embodiments of the present disclosure and features of the embodiments may be combined with each other to arrive at new embodiments.
Example 1
The automatic liquid-separating and adding device shown in the figures 1-6 comprises a driving platform 1, a liquid-transferring gun 2, a reagent placing area 3, a sample placing area 4 and a horizontal table top 5; drive platform 1, pipetting gun 2, reagent place district 3, sample place district 4, set up in on the horizontal table surface 5, pipetting gun 2 sets up drive platform 1 is last, drive platform 1 realizes pipetting gun 2 follows reagent places district 3 to the reciprocating motion of sample place district 4.
The driving platform 1 comprises a first direction shaft 11 and a second direction shaft 12, the first direction shaft 11 and the second direction shaft 12 are arranged perpendicular to each other, the second direction shaft 12 is arranged on the first direction shaft 11, and the moving directions of the first direction shaft 11 and the second direction shaft 12 are both parallel to the horizontal table top 5.
The driving platform 1 further comprises a third direction shaft 13, the third direction shaft 13 is disposed at an end position of the second direction shaft 12, and a moving direction of the third direction shaft 13 is perpendicular to the horizontal table top 5.
The reagent storage device is characterized by further comprising a gun head placing area 6, wherein the gun head placing area 6 is arranged on the horizontal table top 5, and the gun head placing area 6 is adjacent to the reagent storage area 3.
The driving platform 1 realizes the sequential reciprocating movement of the pipette 2 from the pipette head placing area 6, through the reagent placing area 3 and to the sample placing area 4.
The automatic feeding device is characterized by further comprising a gun head recovery box 7, wherein the gun head recovery box 7 is arranged on the horizontal table top 5.
The driving platform 1 realizes the sequential reciprocating movement of the pipette 2 from the pipette head placing area 6, through the reagent placing area 3, through the sample placing area 4 and to the pipette head recovery box 7.
The liquid-transfering gun 2 comprises a motor and a piston, wherein the motor is connected with the piston through a transmission device.
The liquid-transferring gun is characterized by further comprising a control mechanism 8, wherein a control system is arranged in the control mechanism 8, and the control system is connected with the driving platform 1 and the liquid-transferring gun 2; the control mechanism 8 is a computer touch screen.
And the horizontal table top 5 is provided with a mounting assembly fixedly connected with the driving platform 1, the liquid-transferring gun 2, the reagent placing area 3, the sample placing area 4, the gun head placing area 6 and the gun head recovery box 7.
Example 2
The automatic liquid-separating and adding device shown in the figures 1-6 comprises a driving platform 1, a liquid-transferring gun 2, a reagent placing area 3, a sample placing area 4 and a horizontal table top 5; drive platform 1, pipetting gun 2, reagent place district 3, sample place district 4, set up in on the horizontal table surface 5, pipetting gun 2 sets up drive platform 1 is last, drive platform 1 realizes pipetting gun 2 follows reagent places district 3 to the reciprocating motion of sample place district 4.
The driving platform 1 comprises a first direction shaft 11 and a second direction shaft 12, the first direction shaft 11 and the second direction shaft 12 are arranged perpendicular to each other, the second direction shaft 12 is arranged on the first direction shaft 11, and the moving directions of the first direction shaft 11 and the second direction shaft 12 are both parallel to the horizontal table top 5.
The driving platform 1 further comprises a third direction shaft 13, the third direction shaft 13 is disposed at an end position of the second direction shaft 12, and a moving direction of the third direction shaft 13 is perpendicular to the horizontal table top 5.
The reagent storage device is characterized by further comprising a gun head placing area 6, wherein the gun head placing area 6 is arranged on the horizontal table top 5, and the gun head placing area 6 is adjacent to the reagent storage area 3.
The driving platform 1 realizes the sequential reciprocating movement of the pipette 2 from the pipette head placing area 6, through the reagent placing area 3 and to the sample placing area 4.
The liquid-transfering gun 2 comprises a motor and a piston, wherein the motor is connected with the piston through a transmission device.
The liquid-transferring gun is characterized by further comprising a control mechanism 8, wherein a control system is arranged in the control mechanism 8, and the control system is connected with the driving platform 1 and the liquid-transferring gun 2; the control mechanism 8 is a computer touch screen.
And the horizontal table top 5 is provided with a mounting assembly fixedly connected with the driving platform 1, the liquid-transferring gun 2, the reagent placing area 3, the sample placing area 4 and the gun head placing area 6.
Example 3
The automatic liquid-separating and adding device shown in the figures 1-6 comprises a driving platform 1, a liquid-transferring gun 2, a reagent placing area 3, a sample placing area 4 and a horizontal table top 5; drive platform 1, pipetting gun 2, reagent place district 3, sample place district 4, set up in on the horizontal table surface 5, pipetting gun 2 sets up drive platform 1 is last, drive platform 1 realizes pipetting gun 2 follows reagent places district 3 to the reciprocating motion of sample place district 4.
The driving platform 1 comprises a first direction shaft 11 and a second direction shaft 12, the first direction shaft 11 and the second direction shaft 12 are arranged perpendicular to each other, the second direction shaft 12 is arranged on the first direction shaft 11, and the moving directions of the first direction shaft 11 and the second direction shaft 12 are both parallel to the horizontal table top 5.
The driving platform 1 further comprises a third direction shaft 13, the third direction shaft 13 is disposed at an end position of the second direction shaft 12, and a moving direction of the third direction shaft 13 is perpendicular to the horizontal table top 5.
The liquid-transfering gun 2 comprises a motor and a piston, wherein the motor is connected with the piston through a transmission device.
The liquid-transferring gun is characterized by further comprising a control mechanism 8, wherein a control system is arranged in the control mechanism 8, and the control system is connected with the driving platform 1 and the liquid-transferring gun 2; the control mechanism 8 is a computer touch screen.
And the horizontal table top 5 is provided with a mounting assembly fixedly connected with the driving platform 1, the liquid-transferring gun 2, the reagent placing area 3 and the sample placing area 4.
It should be finally noted that the above only serves to illustrate the technical solution of the present invention, and not to limit the scope of the present invention, and that simple modifications or equivalent replacements performed by those skilled in the art to the technical solution of the present invention do not depart from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. An automatic liquid-separating and adding device is characterized by comprising a driving platform (1), a liquid-transferring gun (2), a reagent placing area (3), a sample placing area (4) and a horizontal table top (5); drive platform (1), pipetting gun (2), reagent place district (3), sample place district (4), set up in on horizontal table face (5), pipetting gun (2) set up on drive platform (1), drive platform (1) is realized pipetting gun (2) is followed reagent places district (3) to the reciprocating motion of sample placement district (4).
2. The automatic liquid distribution and adding device according to claim 1, wherein the driving platform (1) comprises a first direction shaft (11) and a second direction shaft (12), the first direction shaft (11) and the second direction shaft (12) are arranged perpendicularly to each other, the second direction shaft (12) is arranged on the first direction shaft (11), and the moving directions of the first direction shaft (11) and the second direction shaft (12) are both arranged in parallel with the horizontal table top (5).
3. Automatic device for dispensing and adding liquid according to claim 2, characterized in that said driving platform (1) further comprises a third direction shaft (13), said third direction shaft (13) being arranged in an end position of said second direction shaft (12), the direction of movement of said third direction shaft (13) being perpendicular to said horizontal table (5).
4. The automatic liquid separating and adding device according to claim 1, further comprising a lance tip placing area (6), wherein the lance tip placing area (6) is arranged on the horizontal table top (5), and the lance tip placing area (6) is arranged adjacent to the reagent placing area (3).
5. The automatic liquid separating and adding device according to claim 4, wherein the driving platform (1) realizes the sequential reciprocating movement of the liquid-transferring gun (2) from the gun head placing area (6), through the reagent placing area (3) and to the sample placing area (4).
6. The automatic liquid-separating and adding device according to claim 4, further comprising a gun head recovery box (7), wherein the gun head recovery box (7) is arranged on the horizontal table top (5).
7. The automatic liquid separating and adding device according to claim 6, wherein the driving platform (1) realizes sequential reciprocating movement of the liquid-transferring gun (2) from the gun head placing area (6), through the reagent placing area (3), through the sample placing area (4) and to the gun head recovery box (7).
8. The automatic liquid separating and adding device according to claim 1, wherein the liquid-transferring gun (2) comprises a motor and a piston, and the motor is connected with the piston through a transmission device.
9. The automatic liquid separating and adding device according to claim 1, further comprising a control mechanism (8), wherein a control system is arranged in the control mechanism (8), and the control system is connected with the driving platform (1) and the liquid-transferring gun (2); the control mechanism (8) is a computer touch screen.
10. The automatic liquid separating and adding device according to any one of claims 1 to 9, wherein the horizontal table top (5) is provided with a mounting assembly fixedly connected with the driving platform (1), the liquid transferring gun (2), the reagent placing area (3), the sample placing area (4), the gun head placing area (6) and the gun head recycling box (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120802347.3U CN215250859U (en) | 2021-04-19 | 2021-04-19 | Automatic liquid-separating and adding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120802347.3U CN215250859U (en) | 2021-04-19 | 2021-04-19 | Automatic liquid-separating and adding device |
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| Publication Number | Publication Date |
|---|---|
| CN215250859U true CN215250859U (en) | 2021-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202120802347.3U Active CN215250859U (en) | 2021-04-19 | 2021-04-19 | Automatic liquid-separating and adding device |
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| CN (1) | CN215250859U (en) |
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- 2021-04-19 CN CN202120802347.3U patent/CN215250859U/en active Active
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