CN215209494U - Automatic cell culture platform - Google Patents

Abstract

The utility model provides an automatic cell culture platform, which comprises a B-level platform body, wherein the B-level platform body is transmitted with an A-level operation area through an automatic transmission window, the B-level platform body comprises a culture area, a cold storage area, an artificial transmission window and an aseptic robot provided with a track, and the robot realizes automatic operation in the B-level platform body through the robot track; the robot guide rail is arranged at the bottom of the platform body and is in linear transverse arrangement, and the robot moves back and forth through the guide rail. The scheme has the beneficial effects that: the material transfer between the B-level platform body and the A-level operation area and the outside is realized through the transfer windows, so that the transfer is flexible and convenient; the automation of transmission is realized through a robot, the cell factory group is placed in a culture area, manual operation is not needed, the working efficiency is improved, and the whole cell factory group is in a sterile environment; the cell culture realizes the automatic monitoring and management function, and automatically requests operations such as liquid replacement in the later period according to the cell culture condition.

Description

Automatic cell culture platform

Technical Field

The utility model relates to an automatic culture apparatus, concretely relates to automatic cell culture platform.

Background

Adherent cells are attached to the surface of a culture dish during culture and can continuously proliferate until the cells are spread on the surface of the whole cell factory, and then the adherent cells can be digested from the cell factory.

The current cell culture process is manual operation, after a culture solution and cells thereof are filled into a cell factory, the cell factory is manually placed into an incubator for culture, and after the culture is finished, the cell factory is taken out by an operator to change the culture solution and then is manually placed into the incubator for culture.

The whole process is manually operated, the operation is unstable, and a plurality of factory groups are operated at the same time, so that the labor intensity is high and the efficiency is low. Therefore, there is a need to develop a new cell culture platform to automatically culture cells in a cell factory without manual operation, reduce the risk of contamination, achieve a sterile environment, and improve efficiency, thereby reducing production cost.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a new culture platform realizes that the cell need not manual operation at the automatic culture of cell work, reduces the pollution risk, and improves production efficiency.

To achieve the above objective, the present invention provides the following technical solutions: an automatic cell culture platform comprises a B-level platform body, wherein the B-level platform body is transferred with an A-level operation area through an automatic transfer window, the B-level platform body comprises a culture area, a cold storage area, an artificial transfer window and a sterile robot provided with a track, and the robot realizes automatic operation in the B-level platform body through the robot track;

the robot guide rail is arranged at the bottom of the platform body and is linearly and transversely arranged, the robot moves back and forth through the guide rail, and the robot arm and the gripper can reach any working area of the B-level platform body;

the manual transmission window is set as at least one window;

the automatic transfer windows are arranged in two modes and are communicated with the B-level platform body and the A-level operation area, and therefore material transfer is achieved.

The further improvement is that the robot guide rail is made of stainless steel.

The robot is further improved in that the robot is fixedly connected to the guide plate platform, and the guide plate platform is arranged corresponding to the robot track, so that the robot on the guide plate platform can move back and forth on the guide rail.

In a further improvement, the culture area is provided with a plurality of culture boxes.

The further improvement is that the refrigeration area is provided with a single refrigeration box, and the refrigeration box is a refrigerator setting with constant temperature control at 4 degrees.

The further improvement lies in that the inner door and the outer door on the incubator are respectively provided with a handle which can be opened and closed by a robot

The further improvement is that the incubator and the refrigerator are independently controlled by power supply.

The further improvement lies in that a distance measuring sensor, a monitoring camera and a quick-change chuck are arranged at the robot gripper, and the opening of the door handles of the window and the incubator is realized through the chuck.

The further improvement lies in that a monitoring camera is arranged in the B-stage platform body, and the whole internal environment can be observed.

According to the technical scheme, the technical scheme of the invention provides the following beneficial effects:

firstly, material transfer between the B-level platform body and the A-level operation area and the outside is realized through a transfer window, and the transfer is flexible and convenient;

secondly, the automation of transmission is realized through a robot, the cell factory group is placed in a culture area, manual operation is not needed, the working efficiency is improved, the whole cell factory group is in a sterile environment, and the safety performance is enhanced;

thirdly, the full-automatic unmanned operation of cell culture is realized, the automatic monitoring management function of the cell culture is realized, and the operations such as liquid replacement in the later period are automatically requested according to the cell culture condition.

It should be understood that all combinations of the foregoing concepts and additional concepts described in greater detail below can be considered as part of the inventive subject matter of the present disclosure unless such concepts are mutually inconsistent.

The foregoing and other aspects, embodiments and features of the present teachings can be more fully understood from the following description taken in conjunction with the accompanying drawings. Additional aspects of the present invention, such as features and/or advantages of exemplary embodiments, will be apparent from the description which follows, or may be learned by practice of the specific embodiments in accordance with the teachings of the present invention.

Drawings

The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures may be represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing. Embodiments of various aspects of the present invention will now be described, by way of example, with reference to the accompanying drawings, in which:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of FIG. 1;

FIG. 3 is a schematic structural view of a robot arm gripper;

FIG. 4 is a schematic structural view of portion A of FIG. 2;

the system comprises a robot track, a distance measuring sensor, a monitoring camera, a quick-change chuck and a guide plate platform, wherein the robot track comprises 1-an automatic transfer window, 2-a culture area, 3-a cold storage area, 4-a manual transfer window, 5-the robot track, 6-the distance measuring sensor, 7-the monitoring camera, 8-the quick-change chuck and 9-the guide plate platform.

Detailed Description

In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. All other embodiments, which can be obtained by a person skilled in the art without any inventive work based on the described embodiments of the present invention, belong to the protection scope of the present invention. Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs.

The use of "first," "second," and similar terms in the description and in the claims does not indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Similarly, the singular forms "a," "an," or "the" do not denote a limitation of quantity, but rather denote the presence of at least one, unless the context clearly dictates otherwise. The terms "comprises," "comprising," or the like, mean that the elements or items listed before "comprises" or "comprising" encompass the features, integers, steps, operations, elements, and/or components listed after "comprising" or "comprising," and do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. "upper", "lower", "left", "right", and the like are used only to indicate relative positional relationships, and when the absolute position of the object to be described is changed, the relative positional relationships may also be changed accordingly.

Embodiment 1, with reference to fig. 1-2, an automatic cell culture platform includes a B-stage platform body, the B-stage platform body is transferred through an automatic transfer window 1 and an a-stage operation area, a culture area 2, a cold storage area 3, an artificial transfer window 4 and a sterile robot are arranged inside the B-stage platform body, and the robot realizes automatic operation in the B-stage platform body through a robot track 5; referring to the attached drawing 4 in detail, the robot is controlled automatically, the robot guide rail 5 is arranged at the bottom of the platform body and is arranged linearly and transversely, and the robot guide rail 5 is made of stainless steel; the robot is fixedly connected to the guide plate platform 9, the guide plate platform 9 is arranged corresponding to the robot track 5, so that the robot on the guide plate platform can move back and forth on the guide rail, the robot arm gripper can reach any working area of the B-stage platform body, and the guide rail is controlled by the servo motor. Furthermore, an observation window can be arranged on the B-stage platform body, the observation window is arranged as an airtight glass window, and the internal state of the working area can be observed through the observation window, so that an appropriate working step can be selected.

In embodiment 2, with reference to fig. 1 and fig. 3, the manual transfer window 4 is provided as at least one window, the B-stage platform body performs material transfer with the outside through the manual transfer window 4, the automatic transfer windows 1 are provided in two, and the automatic transfer windows 1 communicate the B-stage platform body with the a-stage operation area to realize material transfer; the culture area 2 is provided with a plurality of culture boxes, the refrigerating area 3 is provided with a single refrigerating box, and the refrigerating box is arranged in a 4-DEG C constant-temperature refrigerator. Be equipped with interior door and outer door on the incubator, be equipped with the display screen on the outer door to be equipped with the handle respectively on interior door and outer door, open and close through the chuck on the robot. The incubator and the refrigerating box are independently controlled by power supply, and the incubator is provided with a temperature data monitoring system for real-time data monitoring. And a distance measuring sensor 6, a monitoring camera 7 and a quick-change chuck 8 are arranged at the robot arm gripper, and the material taking and the door handle opening of the culture area and the door handle opening of the cold storage area are realized through the robot arm gripper. The distance measuring sensor and the monitoring camera are matched with each other, so that position detection, transmission of materials in the B-level platform body and the regional condition of the materials are achieved to the monitoring system respectively, and real-time monitoring is achieved. The B-level platform body is provided with a safety door lock, so that the B-level platform body is in an aseptic environment, and a cell production master control cabinet is arranged outside the B-level platform body to control the operation of the AB area. A monitoring camera can be arranged in the B-stage platform body, and the whole internal environment can be observed. Furthermore, be equipped with between the AB district and lead to the door, lead to the door and adopt safe lock, make AB district be in aseptic isolation environment each other, the lock is opened, the two regional link up of AB of being convenient for.

The working process is as follows: placing a plurality of culture boxes at fixed positions of a culture area, introducing media such as a power supply, carbon dioxide and the like, carrying out material transfer on a B-level platform body and the outside through a manual transfer window 4, and carrying out material transfer on the B-level platform body and an A-level operation area through an automatic transfer window 1; the incubator is provided with interior door and outer door, all opens and closes automatically through robot control, and the robot guide 5 of sharp horizontal arrangement can realize that aseptic robot removes at A level operation district, and the guide rail adopts servo motor control, and automatic control's robot enlarges the regional reach range of B level by a wide margin, realizes that cell factory takes out and puts into and opening and closing of door handle at the incubator. The B-level platform body is matched with the action of the transfer window, so that full-automatic unmanned operation of cell culture is realized, automatic monitoring and management functions are realized for cell culture, and operations such as liquid replacement in the later period are automatically requested according to cell culture conditions.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention. The present invention is intended to cover by those skilled in the art various modifications and adaptations of the invention without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention is subject to the claims.

Claims (8)

1. An automatic cell culture platform comprises a B-level platform body, wherein the B-level platform body is transferred with an A-level operation area through an automatic transfer window (1), and is characterized in that the B-level platform body comprises a culture area (2), a cold storage area (3), an artificial transfer window (4) and a sterile robot provided with a track, and the robot realizes automatic operation in the B-level platform body through a robot track (5);

the robot track (5) is arranged at the bottom of the platform body and is linearly and transversely arranged, and the robot moves back and forth through the guide rail;

the manual transmission window (4) is arranged as at least one window;

the automatic transfer windows (1) are arranged in two ways, and the automatic transfer windows (1) are communicated with the B-level platform body and the A-level operation area to realize material transfer.

2. The automatic cell culture platform according to claim 1, wherein the robot rail (5) is made of stainless steel.

3. The automatic cell culture platform according to claim 1, wherein the robot is fixedly connected to the guide plate platform (9), and the guide plate platform (9) is arranged corresponding to the robot track (5), so that the robot on the guide plate platform can move back and forth on the guide rail.

4. An automated cell culture platform according to claim 1, wherein the culture section (2) is provided with a plurality of culture boxes, and the cold storage section (3) is provided with a single cold storage box.

5. The automatic cell culture platform of claim 4, wherein the side of the stage B platform body located at the manual transmission window (4) is provided with an observation window, and the observation window is made of glass.

6. The automatic cell culture platform as claimed in claim 5, wherein the robot arm gripper is provided with a distance measuring sensor (6), a monitoring camera (7) and a quick-change chuck (8), and the robot arm gripper is used for realizing material taking and opening or closing of doors of the culture area and the cold storage area.

7. The automatic cell culture platform of claim 6, wherein the inner door and the outer door of the incubator are respectively provided with a handle, and the inner door and the outer door are opened and closed by a robot.

8. The automatic cell culture platform of claim 7, wherein the B-stage platform body is provided with a safety door lock, and a cell production master control cabinet is arranged outside the B-stage platform body to control the operation of A, B.

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