CN215162772U

CN215162772U - Magnetic separation device for fixing enzyme

Info

Publication number: CN215162772U
Application number: CN202023011561.1U
Authority: CN
Inventors: 朱丹
Original assignee: Guangzhou Kefu Technology Co ltd
Current assignee: Guangzhou Kefu Technology Co ltd
Priority date: 2020-12-15
Filing date: 2020-12-15
Publication date: 2021-12-14
Anticipated expiration: 2030-12-15

Abstract

The utility model discloses a magnetic separation device for fixed enzyme relates to fixed enzyme processing field, including the separator box, the top of separator box is provided with the apron, the bottom fixedly connected with saddle of apron, the bottom of apron is provided with the seal box, the top of seal box inner chamber is provided with driving motor, driving motor's middle part cover is equipped with the mounting bracket, the bottom of seal box is provided with the axis of rotation, the top cover of axis of rotation is equipped with sealed bearing, two spliced poles of the bottom symmetry fixedly connected with of axis of rotation both sides, the bottom symmetry of axis of rotation both sides is provided with two mounting panels, the top and the bottom symmetry fixedly connected with magnetic stripe of mounting panel, the mounting panel is close to the centre of axis of rotation one side and has seted up the fixed slot, the notch department of fixed slot is provided with the sealing washer. A magnetic separation device for immobilized enzyme, through will stir the structure and install on the apron, reduce the volume of separator box, it is convenient to use for the installation of separator box.

Description

Magnetic separation device for fixing enzyme

Technical Field

The utility model relates to a fixed enzyme processing field, in particular to a magnetic separation device for fixed enzyme.

Background

The protease has the advantages of high substrate specificity and high hydrolysis rate, thereby playing an increasingly important role in the field of biological pharmacy. The magnetic separation technology is a technology for carrying out solid-liquid phase separation on a magnetic particle compound and a reaction liquid, protease covalent bonds can be formed on the surface of a hydrophilic carrier, high-concentration enzyme on the surface of the carrier can carry out rapid protein enzymolysis, the protein hydrolysis time is obviously shortened, and the magnetic separation effect is ensured. In the separation process, the magnetic particle compound needs to be subjected to liquid injection, uniform mixing and liquid absorption processes for multiple times so as to achieve a good separation and cleaning effect, and the magnetic particle compound is tightly adsorbed on the side wall of the reaction cup through the magnet so as to prevent the compound from being absorbed away, thereby obtaining an accurate test result.

The existing magnetic separation structure mostly fixes the magnet used for adsorbing the magnetic particle compound outside the structure, so that the magnet is needed to have stronger adsorption strength, the magnet is thicker, and the assembly occupies larger space, so that the instrument is larger in size and higher in cost.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a main aim at provides a magnetic separation device for fixed enzyme, through with stirring the structure installation on the apron, reduces the volume of separator box, and it is convenient for the installation of separator box is used, can effectively solve the problem in the background art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a magnetic separation device for fixing enzyme comprises a separation box, wherein a cover plate is arranged at the top of the separation box, a supporting platform is fixedly connected at the bottom of the cover plate, a seal box is arranged at the bottom of the cover plate, a driving motor is arranged at the top of an inner cavity of the seal box, a mounting frame is sleeved at the middle part of the driving motor, a rotating shaft is arranged at the bottom of the seal box, a sealing bearing is sleeved at the top end of the rotating shaft, two connecting columns are symmetrically and fixedly connected at the bottoms of two sides of the rotating shaft, two mounting plates are symmetrically and fixedly arranged at the bottoms of two sides of the rotating shaft, magnetic stripes are symmetrically and fixedly connected at the tops and the bottoms of the mounting plates, a fixing groove is formed in the center of one side, close to the rotating shaft, of the mounting plates, a sealing ring is arranged at the notch of the fixing groove, clamping plates are symmetrically arranged at the tops and the bottoms of the inner cavities of the fixing grooves, and three guide rods are respectively and fixedly connected at one side, which is opposite to each other, one end of the guide rod close to the clamping plate is sleeved with a compression spring.

Preferably, the inner chamber of separator box is linked together by its top and external world, the saddle sets up at the bottom ring shape of apron, the top of seal box extends to the inside of saddle inner circle, and the top fixed connection of seal box is on the outer wall of apron bottom, the outer wall at separator box top runs through the outer wall of saddle bottom and extends to the inside of saddle wall body, the wall body of separator box laminating saddle, and the seal box extends to the inside of separator box by the opening at separator box top.

Preferably, driving motor and the close laminating of the wall body of mounting bracket and fixed connection, the top fixed connection of mounting bracket is on the inner wall at seal box top, the centre of driving motor bottom is extended there is the output shaft, the top of axis of rotation runs through the outer wall of seal box bottom and extends to the inside of seal box, sealed bearing sets up between the wall body of axis of rotation and seal box, and sealed bearing closely laminates and fixed connection with the wall body of seal box, and the axis of rotation closely laminates with sealed bearing's wall body, and the top of axis of rotation is connected with the bottom of output shaft.

Preferably, the mounting panel corresponds the setting with the spliced pole, the inner wall and the fixed connection of the wall body laminating fixed slot of sealing washer outer lane, the one end that the axis of rotation was kept away from to the spliced pole runs through the sealing washer that corresponds respectively and extends to the inside of fixed slot, and the spliced pole setting is between two splint, the wall body of spliced pole laminating sealing washer.

Preferably, the front surface of the mounting plate is provided with a fixing bolt, and the connecting column is fixedly connected with the wall body of the mounting plate through the fixing bolt.

Preferably, the other end of guide bar runs through the inner wall of fixed slot and extends to the inside of mounting panel wall body, the wall body of guide bar laminating mounting panel, compression spring's one end fixed connection is on splint, and compression spring's other end fixed connection is on the inner wall of fixed slot.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model discloses in, it is rotatory at a low speed to drive the axis of rotation through driving motor, adsorb magnetic particle complex with the stirring of magnetic stripe in the separator box inside, through installing stirring structure on the apron, reduce the volume of separator box, it is convenient for the installation and use of separator box provides, carry out the centre gripping to the spliced pole through splint, with the guide bar, compression spring is for removing the reduction, provide the buffering for splint, when mounting panel contact water liquid swings, provide the space of swaing for the mounting panel through buffer area, avoid the too big and fracture of mounting panel atress, sealing washer through setting up seals between spliced pole and the fixed slot, and the sealing washer is to having elastic deformation nature, the degree of compliance is good, avoid the fixed slot inside to intake and lead to the compression spring corrosion.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is an enlarged view of the present invention at A in FIG. 1;

fig. 3 is an enlarged view of the present invention at B in fig. 2.

In the figure: 1. a separation tank; 2. a cover plate; 3. a saddle; 4. a sealing box; 5. a drive motor; 6. a mounting frame; 7. a rotating shaft; 8. sealing the bearing; 9. connecting columns; 10. mounting a plate; 11. a magnetic strip; 12. fixing grooves; 13. a seal ring; 14. a splint; 15. a guide bar; 16. compressing the spring.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element to which the reference is made must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1-3, a magnetic separation device for fixing enzyme, including separator 1, separator 1's top is provided with apron 2, the bottom fixedly connected with saddle 3 of apron 2, the bottom of apron 2 is provided with seal box 4, separator 1's inner chamber is linked together with the external world by its top, saddle 3 is at the annular setting in the bottom of apron 2, seal box 4's top extends to the inside of saddle 3 inner circle, seal box 4's top fixed connection is on the outer wall of apron 2 bottom, the outer wall at separator 1 top runs through the outer wall of saddle 3 bottom and extends to the inside of saddle 3 wall body, separator 1 laminates the wall body of saddle 3, seal box 4 extends to separator 1's inside by the opening at separator 1 top.

The top of the inner cavity of the seal box 4 is provided with a driving motor 5, the middle part of the driving motor 5 is sleeved with a mounting frame 6, the bottom of the seal box 4 is provided with a rotating shaft 7, the top end of the rotating shaft 7 is sleeved with a sealing bearing 8, the driving motor 5 is tightly attached and fixedly connected with the wall body of the mounting frame 6, the top end of the mounting frame 6 is fixedly connected on the inner wall of the top of the seal box 4, an output shaft extends from the center of the bottom of the driving motor 5, the top end of the rotating shaft 7 penetrates through the outer wall of the bottom of the seal box 4 and extends into the seal box 4, the sealing bearing 8 is arranged between the rotating shaft 7 and the wall body of the seal box 4, the sealing bearing 8 is tightly attached and fixedly connected with the wall body of the seal box 4, the rotating shaft 7 is tightly attached with the wall body of the sealing bearing 8, the top end of the rotating shaft 7 is connected with the bottom end of the output shaft, the rotating shaft 7 is driven to rotate at a low speed by the driving motor 5, adsorb magnetic particle compound with stirring of magnetic stripe 11 in separator box 1 inside, through installing stirring structure on apron 2, reduce separator box 1's volume, provide convenient for separator box 1's installation is used.

Two connecting columns 9 are symmetrically and fixedly connected to the bottoms of two sides of the rotating shaft 7, two mounting plates 10 are symmetrically and fixedly arranged at the bottoms of two sides of the rotating shaft 7, magnetic stripes 11 are symmetrically and fixedly connected to the tops and the bottoms of the mounting plates 10, a fixing groove 12 is formed in the center of one side, close to the rotating shaft 7, of each mounting plate 10, a sealing ring 13 is arranged at a notch of each fixing groove 12, clamping plates 14 are symmetrically arranged at the tops and the bottoms of inner cavities of the fixing grooves 12, the mounting plates 10 are arranged corresponding to the connecting columns 9, the wall body of the outer ring of each sealing ring 13 is attached to the inner wall of the corresponding fixing groove 12 and fixedly connected to the inner wall of the corresponding sealing ring 13, one end, far away from the rotating shaft 7, of each connecting column 9 penetrates through the corresponding sealing ring 13 and extends into the corresponding fixing groove 12, fixing bolts are arranged on the front surfaces of the mounting plates 10, the connecting columns 9 are fixedly connected with the wall body of the mounting plates 10 through the fixing bolts, the connecting columns 9 are arranged between the two clamping plates 14, and the connecting columns 9 are attached to the wall body of the sealing rings 13, carry out the centre gripping to spliced pole 9 through splint 14, with guide bar 15, compression spring 16 is for removing the reduction, for splint 14 provides the buffering, when mounting panel 10 contact water liquid swing, provide the space of swaing for mounting panel 10 through the buffer area, avoid mounting panel 10 atress too big and fracture, seal up between spliced pole 9 and fixed slot 12 through the sealing washer 13 that sets up, and sealing washer 13 is to having elastic deformation nature, it is good to be docile, avoid the inside intaking of fixed slot 12 and lead to compression spring 16 corrosion.

Two splint 14 one side that carry on the back mutually respectively three guide bar 15 of fixedly connected with, the pot head that guide bar 15 is close to splint 14 is equipped with compression spring 16, and the other end of guide bar 15 runs through the inner wall of fixed slot 12 and extends to the inside of the wall body of mounting panel 10, and the wall body of mounting panel 10 is laminated to guide bar 15, and compression spring 16's one end fixed connection is on splint 14, and compression spring 16's other end fixed connection is on the inner wall of fixed slot 12.

It is noted that the utility model is a magnetic separation device for fixing enzyme, when in use, the rotating shaft 7 is driven by the driving motor 5 to rotate at a low speed, the magnetic particle compound is adsorbed by the stirring of the magnetic strip 11 in the separation box 1, the stirring structure is arranged on the cover plate 2, so that the volume of the separation box 1 is reduced, the installation and the use of the separation box 1 are convenient, the connecting column 9 is clamped by the clamping plate 14, the guide rod 15 and the compression spring 16 are used for reducing movement, the clamping plate 14 is provided with buffering, when the mounting plate 10 swings in contact with water, the buffer area provides a swing space for the mounting plate 10, so that the mounting plate 10 is prevented from being broken due to over-stress, seal up between spliced pole 9 and fixed slot 12 through the sealing washer 13 that sets up, and sealing washer 13 is to having elastic deformation nature, and the compliance is good, avoids the inside intaking of fixed slot 12 and leads to compression spring 16 corrosion.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A magnetic separation device for immobilized enzymes, comprising a separation box (1), characterized in that: the top of separator box (1) is provided with apron (2), the bottom fixedly connected with saddle (3) of apron (2), the bottom of apron (2) is provided with seal box (4), the top of seal box (4) inner chamber is provided with driving motor (5), the middle part cover of driving motor (5) is equipped with mounting bracket (6), the bottom of seal box (4) is provided with axis of rotation (7), the top cover of axis of rotation (7) is equipped with sealed bearing (8), the bottom symmetry fixedly connected with two spliced poles (9) of axis of rotation (7) both sides, the bottom symmetry of axis of rotation (7) both sides is provided with two mounting panels (10), the top and the bottom symmetry fixedly connected with magnetic stripe (11) of mounting panel (10), fixed slot (12) have been seted up to mounting panel (10) near the centre of axis of rotation (7) one side, the notch department of fixed slot (12) is provided with sealing washer (13), the top and the bottom symmetry of fixed slot (12) inner chamber are provided with splint (14), and two splint (14) one side of carrying on the back mutually are three guide bar (15) of fixedly connected with respectively, and the pot head that guide bar (15) are close to splint (14) is equipped with compression spring (16).

2. A magnetic separation device for immobilizing enzyme according to claim 1, wherein: the inner chamber of separator box (1) is linked together by its top and external world, saddle (3) are in the bottom annular setting of apron (2), the top of seal box (4) extends to the inside of saddle (3) inner circle, and the top fixed connection of seal box (4) is on the outer wall of apron (2) bottom, the outer wall at separator box (1) top runs through the outer wall of saddle (3) bottom and extends to the inside of saddle (3) wall body, and the wall body of saddle (3) is laminated to separator box (1), and seal box (4) extend to the inside of separator box (1) by the opening at separator box (1) top.

3. A magnetic separation device for immobilizing enzyme according to claim 1, wherein: driving motor (5) and the close laminating of the wall body of mounting bracket (6) and fixed connection, the top fixed connection of mounting bracket (6) is on the inner wall at seal box (4) top, the centre of driving motor (5) bottom extends there is the output shaft, the top of axis of rotation (7) runs through the outer wall of seal box (4) bottom and extends to the inside of seal box (4), sealed bearing (8) set up between the wall body of axis of rotation (7) and seal box (4), and sealed bearing (8) and the close laminating of the wall body of seal box (4) and fixed connection, and axis of rotation (7) and the wall body of sealed bearing (8) closely laminate, and the top of axis of rotation (7) is connected with the bottom of output shaft.

4. A magnetic separation device for immobilizing enzyme according to claim 1, wherein: mounting panel (10) correspond the setting with spliced pole (9), the inner wall and the fixed connection of the wall body laminating fixed slot (12) of sealing washer (13) outer lane, the one end that axis of rotation (7) was kept away from in spliced pole (9) runs through corresponding sealing washer (13) respectively and extends to the inside of fixed slot (12), and spliced pole (9) set up between two splint (14), the wall body of spliced pole (9) laminating sealing washer (13).

5. A magnetic separation device for immobilizing enzyme according to claim 4, wherein: the front of mounting panel (10) is provided with fixing bolt, and the wall body of spliced pole (9) and mounting panel (10) passes through fixing bolt fixed connection.

6. A magnetic separation device for immobilizing enzyme according to claim 1, wherein: the other end of guide bar (15) runs through the inner wall of fixed slot (12) and extends to the inside of mounting panel (10) wall body, and the wall body of mounting panel (10) is laminated in guide bar (15), the one end fixed connection of compression spring (16) is on splint (14), and the other end fixed connection of compression spring (16) is on the inner wall of fixed slot (12).