CN213951216U - Immunomagnetic bead automatic separation device - Google Patents

Abstract

The utility model discloses an automatic separating device for immunomagnetic beads, which comprises a fixed chassis, a fixed ring is arranged above the fixed chassis, an electromagnet is arranged inside the fixing ring, a telescopic rod is arranged on one side of the fixing ring, a spongy cushion is arranged inside the fixing ring, the sponge cushion is inserted and connected with a test tube, a test tube cover is arranged above the test tube, a double-layer sealing ring is arranged inside the test tube cover, the rotating motor can rotate the pipette when being started, thereby driving the stirring rod to rotate, stirring the cell suspension added with the immunomagnetic beads to ensure that the magnetic beads can fully adsorb cells with corresponding antigens, the layered cells in the supernatant can be sucked through the arranged liquid sucking port, and the layered cells can be sucked through the arranged test tube cover, can seal through the inside double-deck sealing washer of test tube lid to the mouth of pipe inner wall and the outer wall of test tube when pressing the pressure dish.

Description

Immunomagnetic bead automatic separation device

Technical Field

The utility model relates to an immunology technical field specifically is an immunomagnetic bead autosegregation device.

Background

The immunomagnetic beads are a new immunological technology developed in recent years, combine the special advantages of immobilized reagents with high specificity of immunological reaction, and permeate into various fields of pathology, physiology, pharmacology, microorganism, biochemistry, molecular genetics and the like on the basis of immunology, so that the immunomagnetic beads are more and more widely applied to the aspects of immunoassay, cell separation, biomacromolecule purification, molecular biology and the like.

Most of the existing immunomagnetic bead separation processes are directly operated by using test tubes, but because the adsorption and precipitation of the magnetic beads need a certain time, the top of the test tube is easily infected with bacteria under the condition of no sealing, and the accuracy of the operation result is influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an immunomagnetic bead autosegregation device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: an automatic separation device for immunomagnetic beads comprises a fixing chassis, wherein a fixing ring is arranged above the fixing chassis, an electromagnet is arranged inside the fixing ring, a telescopic rod is arranged on one side of the fixing ring, a spongy cushion is arranged inside the fixing ring, a test tube is inserted and connected inside the spongy cushion, a test tube cover is arranged above the test tube, a double-layer sealing ring is arranged inside the test tube cover, two sealing rings are arranged inside the double-layer sealing ring and are respectively positioned on the inner wall and the outer wall of the top of the test tube, a pressing block is arranged on the top of the test tube cover, a pressing plate is arranged on the top of the pressing block, a pipette is arranged inside the pressing plate in a penetrating manner, a liquid suction port is arranged on one side above the pipette, the liquid suction port is communicated with the pipette, and the upper side of the pipette is fixedly connected with an output shaft of a rotating motor, the rotating motor is fixedly connected with the lifting top plate, and the lifting top plate is fixedly connected with the top end of the telescopic rod.

As a further aspect of the present invention: the top of pipette is provided with the stopper, the stopper is located the intermediate position of rotating electrical machines and imbibition mouth, the electro-magnet with the rotating electrical machines all with external power source electric connection.

The advantage of adopting above-mentioned further scheme is, can prevent after setting up the stopper that the dynamics is too big when pressing from pushing pressure disk and imbibition mouth to the motor to and reserve the connection space of sufficient imbibition pump for the imbibition mouth.

As a further aspect of the present invention: the pipette runs through respectively and presses the pressure dish, presses the briquetting, test tube lid and double-deck sealing washer, just the pipette is located the inside partial surface of test tube and is provided with the puddler that sets up with the pipette is perpendicular.

Adopt the advantage of above-mentioned further scheme to be, can be more abundant to the stirring of device inside behind the setting puddler.

As a further aspect of the present invention: and an observation port is formed in one side of the spongy cushion and the fixing ring and is positioned at the bottom of the test tube.

Adopt the advantage of above-mentioned further scheme to be, set up the viewing aperture and can stir the bottom when there is the sediment in the bottom at first, make suspension and magnetic bead intensive mixing after deposit again.

As a further aspect of the present invention: the electro-magnet is located the bottom setting of test tube, the central point that electro-magnet and test tube all are located solid fixed ring puts the setting.

Adopt the advantage of above-mentioned further scheme to be located by the electro-magnet that sets up the bottom of test tube can avoid making the pipette absorb the composition impure when magnetic field encircles the pipe wall.

As a further aspect of the present invention: the pipette is located the inside sliding connection who presses the pressure dish, presses the briquetting, test tube lid and double-deck sealing washer, the pipette runs through press the position of pressure dish and be provided with the sealing washer, the pipette communicates with the inside of test tube.

Adopt the advantage of above-mentioned further scheme to be, set up the sealing washer and can avoid the weeping, prevent to stir the in-process and take place the seepage, can avoid external bacterium to get into simultaneously.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the rotating motor is arranged, so that the pipette can be rotated when the rotating motor is started, the stirring rod is driven to rotate, the cell suspension added with the immunomagnetic beads is stirred, the immunomagnetic beads can fully adsorb cells with corresponding antigens, the layered cells in the supernatant can be sucked through the arranged pipette port, and the inner wall and the outer wall of the tube opening of the test tube can be sealed through the double-layer sealing ring in the test tube cover when the pressing plate is pressed through the arranged test tube cover;

2. the test tube can be kept stable through the arranged fixing base plate, the test tube can be fixed through the arranged fixing ring and the sponge mat, the test tube is prevented from shaking, the precipitation layering condition of cell suspension can be observed through the arranged observation port, and the precipitation layering of target cells can be more definite through the arrangement of the electromagnet positioned at the bottom of the test tube;

3. through the telescopic link that sets up, the pipette extends to the inside of test tube until the bottom when pushing down the telescopic link, starts the rotating electrical machines and can stir, can drag the test tube lid out through the puddler during tensile telescopic link.

Drawings

FIG. 1 is a schematic diagram of a front view of an immunomagnetic bead automatic separation device;

FIG. 2 is a schematic view of the internal structure of a device for the automatic separation of immunomagnetic beads in a front view;

FIG. 3 is an enlarged schematic view of the structure A of an immunomagnetic bead automatic separation device;

FIG. 4 is an enlarged schematic view of a structure B of an immunomagnetic bead automatic separation device;

in the figure: 1. fixing the chassis; 2. a fixing ring; 3. a sponge cushion; 4. an electromagnet; 5. a test tube; 6. a telescopic rod; 7. a test tube cover; 8. a double-layer sealing ring; 9. a pipette; 10. a stirring rod; 11. a pressing block; 12. pressing the disc; 13. a liquid suction port; 14. a limiting block; 15. lifting the top plate; 16. a rotating electric machine; 17. a seal ring; 18. a viewing port.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, in the embodiment of the present invention, an automatic immunomagnetic bead separation device comprises a fixing chassis 1, a fixing ring 2 is disposed above the fixing chassis 1, an electromagnet 4 is disposed inside the fixing ring 2, a telescopic rod 6 is disposed on one side of the fixing ring 2, a foam rubber pad 3 is disposed inside the fixing ring 2, the foam rubber pad can fix a test tube and prevent the test tube from shaking, the interior of the foam rubber pad 3 is connected with the test tube 5 in an inserting manner, a test tube cover 7 is disposed above the test tube 5, a double-layer sealing ring 8 is disposed inside the test tube cover 7, two sealing rings are disposed inside the double-layer sealing ring 8, the two sealing rings are respectively disposed on the inner wall and the outer wall of the top of the test tube 5, a pressing block 11 is disposed on the top of the test tube cover 7, a pressing plate 12 is disposed on the top of the pressing block 11, the inner wall and the outer wall of the tube opening of the test tube 5 can be sealed by the double-layer sealing ring 8 inside the test tube cover 7 when the pressing plate 12 is pressed, according to the inside of pressing disc 12 and running through and being provided with pipette 9, one side of pipette 9 top is provided with pipette mouth 13, pipette mouth 13 and pipette 9 intercommunication, can absorb the cell of layering in the supernatant, pipette 9's top and rotating electrical machines 16's output shaft fixed connection, rotating electrical machines 16 and lift roof 15 fixed connection, lift roof 15 and telescopic link 6's top fixed connection, pipette 9 extends to the inside of test tube 5 until the bottom when pushing down telescopic link 6, start rotating electrical machines 16 and can stir, can drag out test tube lid 7 through puddler 10 during tensile telescopic link 6.

Preferably, the top of pipette 9 is provided with stopper 14, and stopper 14 is located the intermediate position of rotating electrical machines 16 and imbibition mouth 13, and electro-magnet 4 and rotating electrical machines 16 all with external power source electric connection make pipette 9 rotate when starting rotating electrical machines 16 to drive puddler 10 and rotate, stir the cell suspension that adds the immunomagnetic bead, make the magnetic bead can fully adsorb the cell that has corresponding antigen.

Preferably, the pipette 9 penetrates through the pressing plate 12, the pressing block 11, the test tube cover 7 and the double-layer sealing ring 8, and the surface of the pipette 9, which is located inside the test tube 5, is provided with a stirring rod 10 which is arranged perpendicular to the pipette 9.

Preferably, one side of the spongy cushion 3 and the fixing ring 2 is provided with an observation port 18, and the observation port 18 is positioned at the bottom of the test tube 5 and can observe the precipitation and stratification conditions of the cell suspension.

Preferably, the electromagnet 4 is arranged at the bottom of the test tube 5, so that the sedimentation and layering of the target cells can be more definite, and the electromagnet 4 and the test tube 5 are both arranged at the center of the fixing ring 2.

Preferably, the pipette 9 is located inside the pressing plate 12, the pressing block 11, the test tube cover 7 and the double-layer sealing ring 8, the sealing ring 17 is arranged at the position where the pipette 9 penetrates through the pressing plate 12, and the pipette 9 is communicated with the inside of the test tube 5.

The utility model discloses a theory of operation is: when in use, the telescopic rod 6 is stretched to the uppermost end, then the test tube 5 containing the cell suspension is placed into the spongy cushion 3, after magnetic beads are put into the interior of the test tube 5, the telescopic rod 6 and the pressing disc 12 are pressed to enable the double-layer sealing ring 8 to seal the tube opening of the test tube 5, the telescopic rod 6 is continuously pressed downwards to enable the lifting top plate 15 to descend, the stirring bar 10 is rotated by starting the rotating motor 16 while the stirring bar 10 is rotated to the bottom of the test tube 5, the electromagnet 4 is communicated with a power supply while the stirring rod 10 rotates, so that the magnetic beads can fully adsorb cells with corresponding antigens, the electromagnet 4 is positioned at the bottom of the test tube 5, so that the sedimentation and the layering of target cells can be more definite, the layered cells in the supernatant are absorbed through the liquid absorbing port 13, or the liquid suction port 13 is extended to the lower part, the target cells on the lower part are sucked and observed, and the test tube cover 7 is pulled out by stretching the telescopic rod 6 after the observation is finished.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims (6)

1. The utility model provides an immunomagnetic bead autosegregation device, includes fixed chassis (1), its characterized in that: a fixing ring (2) is arranged above the fixing chassis (1), an electromagnet (4) is arranged inside the fixing ring (2), a telescopic rod (6) is arranged on one side of the fixing ring (2), a spongy cushion (3) is arranged inside the fixing ring (2), a test tube (5) is inserted and connected inside the spongy cushion (3), a test tube cover (7) is arranged above the test tube (5), a double-layer sealing ring (8) is arranged inside the test tube cover (7), two sealing rings are arranged inside the double-layer sealing ring (8), the two sealing rings are respectively positioned on the inner wall and the outer wall of the top of the test tube (5), a pressing block (11) is arranged at the top of the test tube cover (7), a pressing disc (12) is arranged at the top of the pressing block (11), and a pressing disc (9) is arranged inside the pressing disc (12) in a penetrating manner, one side of pipette (9) top is provided with imbibition mouth (13), imbibition mouth (13) and pipette (9) intercommunication, the top of pipette (9) and the output shaft fixed connection of rotating electrical machines (16), rotating electrical machines (16) and lift roof (15) fixed connection, the top fixed connection of lift roof (15) and telescopic link (6).

2. The device for separating immunomagnetic beads according to claim 1, wherein: the top of the liquid suction pipe (9) is provided with a limiting block (14), the limiting block (14) is located in the middle of the rotating motor (16) and the liquid suction port (13), and the electromagnet (4) and the rotating motor (16) are both electrically connected with an external power supply.

3. The device for separating immunomagnetic beads according to claim 1, wherein: the pipette (9) penetrates through the pressing plate (12), the pressing block (11), the test tube cover (7) and the double-layer sealing ring (8) respectively, and a stirring rod (10) which is perpendicular to the pipette (9) is arranged on the surface of the part, located inside the test tube (5), of the pipette (9).

4. The device for separating immunomagnetic beads according to claim 1, wherein: an observation port (18) is formed in one side of the spongy cushion (3) and the fixing ring (2), and the observation port (18) is located at the bottom of the test tube (5).

5. The device for separating immunomagnetic beads according to claim 1, wherein: electromagnet (4) are located the bottom setting of test tube (5), electromagnet (4) and test tube (5) all are located the central point that solid fixed ring (2) and put the setting.

6. The device for separating immunomagnetic beads according to claim 1, wherein: pipette (9) are located the inside sliding connection who presses disk (12), presses piece (11), test tube lid (7) and double-deck sealing washer (8), pipette (9) run through the position that presses disk (12) is provided with sealing washer (17), pipette (9) and the inside intercommunication of test tube (5).

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