CN215162658U - Scope detects sampling bottle - Google Patents

Scope detects sampling bottle Download PDF

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Publication number
CN215162658U
CN215162658U CN202121650902.1U CN202121650902U CN215162658U CN 215162658 U CN215162658 U CN 215162658U CN 202121650902 U CN202121650902 U CN 202121650902U CN 215162658 U CN215162658 U CN 215162658U
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China
Prior art keywords
sampling
sampling bottle
connector
shaped structure
cap
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CN202121650902.1U
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Chinese (zh)
Inventor
豆清娅
黄勋
吴安华
李洁
付陈超
汪要望
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Xiangya Hospital of Central South University
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Xiangya Hospital of Central South University
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Priority to CN202121650902.1U priority Critical patent/CN215162658U/en
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Abstract

The utility model relates to an endoscope detection sampling bottle, the sampling bottle upper end is equipped with the connector, connector detachable is connected with supplementary sampling subassembly, the sampling bottle lower extreme is equipped with the hole, the utility model discloses sampling bottle unscrews the cap and the funnel-shaped structural connection department on supplementary sampling subassembly upper portion when sampling, puts scope elder generation portion in funnel-shaped structure, directly collects sampling liquid with the sampling bottle, unscrew supplementary sampling subassembly and connector connection department after returning the laboratory, absorb 1mL liquid with the syringe and inoculate on the flat board, then unscrew supplementary sampling subassembly and connector connection department, open the sealing plug silica gel of top and bottom, extract liquid with the vacuum pump, make the microorganism hold back on the filter membrane, paste the filter membrane (holding back face up) on the flat board, cultivate in 36 ℃ incubator, remove longer sampling pipe and peristaltic pump like this compared with the prior art, the sampling is more convenient, the pollution links are reduced, and the operation is more accurate and simple when inoculating 1mL of liquid in a laboratory.

Description

Scope detects sampling bottle
The technical field is as follows:
the utility model relates to a medical supplies technical field relates to an scope detects sampling bottle.
Technical background:
the method for detecting endoscope after disinfection described in the hospital disinfection and health standard (GB 15982-2012) is a membrane filtration method: taking the cleaned and sterilized endoscope, extracting 50mL of eluent containing corresponding neutralizer by using a sterile syringe, injecting the eluent from a biopsy port to wash the lumen of the endoscope, and collecting the whole amount (a peristaltic pump can be used) for inspection. Mixing the eluate, inoculating 1.0mL of the eluate into plates, cooling the molten nutrient agar to 40-45 deg.C, pouring 15-20 mL of agar into each plate, shaking, cooling, solidifying, culturing in 36 + -1 deg.C incubator for 48h, calculating colony count (CFU/piece), filtering the rest eluate with filter membrane (0.45 μm) under aseptic condition, inoculating the filter membrane onto nutrient agar plate, culturing at 36 + -1 deg.C incubator for 48h, and counting colony count.
When the filtration process is not countable: total number of colonies (CFU/piece) ═ m (CFU/plate) × 50
In the formula: m is the average colony number of two parallel plates.
When the filtration membrane process can count: total number of colonies (CFU/piece) ═ m (CFU/plate) + mf (CFU/filter)
Wherein: m is the average colony number of two parallel plates; mf is the number of colonies on the filter.
The collection bottle for domestic detection is common in three types, the first type is that the tip of an endoscope is connected with a tube (increasing the probability of pollution) during sampling, eluent flowing out of the lumen of the endoscope is collected into the bottle through a peristaltic pump and is taken back to a laboratory for treatment, the middle of a connecting tube is unscrewed during laboratory inoculation, and a 1mL syringe is used for sucking liquid at the unscrewing part of the connecting tube for inoculation after disinfection, but the sucking of 1mL is difficult, and the sucking amount is difficult to control accurately; and the second method comprises the following steps: directly pouring the sampled liquid into a cup, and pumping the liquid by using a vacuum pump, and has the defect that the sampled liquid is exposed in the air, so that the pollution is increased; the third one is similar to the first one, but has the disadvantage that the cap on the bottle is opened directly to pour the sample liquid, and the cap is easy to fall off to cause pollution.
The utility model has the following contents:
the utility model aims to provide a: the utility model provides an endoscope detection sampling bottle, it gets rid of longer sampling pipe and peristaltic pump, and is more convenient during the sampling, and has reduced the pollution chance, and this sampling bottle operates more simply accurate when laboratory inoculation 1ml liquid.
In order to solve the technical problem, the utility model discloses a following technical scheme can solve:
the utility model provides an scope detects sampling bottle, the sampling bottle upper end is equipped with the connector, connector detachable is connected with supplementary sampling subassembly, the sampling bottle lower extreme is connected with the connecting seat that can open, connecting seat lower extreme middle part is equipped with the connecting hole that is used for connecting the vacuum pump, in the connecting seat and supplementary sampling subassembly all be equipped with 0.45 mu m's filter membrane.
Preferably, the auxiliary sampling assembly is arranged at the upper end edge of the sampling bottle, the auxiliary sampling assembly comprises a funnel-shaped structure connected with the connector, the upper end of the funnel-shaped structure is detachably connected with a cap, and a 0.45-micrometer filter membrane is arranged in the cap.
Further preferably, the cap is provided with a connecting hole, and the connecting hole at the top of the cap and the connecting hole below the connecting seat are both provided with silica gel sealing plugs.
Further preferably, the connector inner wall is equipped with the internal thread, it is equipped with the external screw thread to leak hopper-shaped structure lower part, connector and the spiro union of leaking hopper-shaped structure.
Further preferably, funnel-shaped structure upper portion is equipped with the internal thread, the cap lower part is equipped with the external screw thread, funnel-shaped structure and cap spiro union.
Further preferably, sampling bottle lower extreme inner wall is equipped with the internal thread, connecting seat upper end outer wall is equipped with the external screw thread, sampling bottle and connecting seat spiro union.
The utility model discloses beneficial effect: comprises a sampling bottle, the upper end of the sampling bottle is provided with a connector, the connector is detachably connected with an auxiliary sampling component, the lower end of the sampling bottle is connected with an openable connecting seat, the middle part of the lower end of the connecting seat is provided with a connecting hole for connecting a vacuum pump, and both the connecting seat and the auxiliary sampling component are provided with 0.45 mu m filter membranes, the utility model discloses a sampling bottle unscrews the joint of the cap and the funnel-shaped structure at the upper part of the auxiliary sampling component during sampling, puts the tip of an endoscope into the funnel-shaped structure, directly collects sampling liquid by the sampling bottle, unscrews the joint of the auxiliary sampling component and the connector after returning to a laboratory, sucks 1mL of liquid by an injector to inoculate on a flat plate, then unscrews the joint of the auxiliary sampling component and the connector, opens the silica gel at the top and the bottom, extracts the liquid by the vacuum pump, and makes microorganisms remained on the filter membranes, the filter membrane (with the interception surface facing upwards) is attached to a flat plate and cultured in an incubator at 36 ℃, so that compared with the prior art, a longer sampling tube and a peristaltic pump are removed, the sampling is more convenient, the pollution links are reduced, and the operation is more accurate and simple when 1mL of liquid is inoculated in a laboratory.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
The specific implementation mode is as follows:
the present invention will be described in further detail with reference to the accompanying drawings and examples.
As shown in fig. 1, the endoscope detection sampling bottle comprises a sampling bottle 1; the sampling device is characterized in that a connector 2 is arranged at the upper end of a sampling bottle 1, an auxiliary sampling assembly is detachably connected with the connector 2, the lower end of the sampling bottle 1 is connected with a connecting seat 6 which can be opened, a connecting hole 3 with a silica gel sealing plug for connecting a vacuum pump is arranged in the middle of the lower end of the connecting seat 6, a filter membrane 7 is arranged in the connecting seat 6, filter membranes with the aperture of 0.45 mu m are arranged in the connecting seat 6 and the cap 5, the auxiliary sampling assembly is arranged at the edge of the upper end of the sampling bottle 1, the auxiliary sampling assembly comprises a funnel-shaped structure 4 connected with the connector 2, the cap 5 is detachably connected with the upper end of the funnel-shaped structure 4, the inner wall of the connector 2 is provided with internal threads, the lower part of the funnel-shaped structure 4 is provided with external threads, and the connector 2 is in threaded connection with the funnel-shaped structure 4; funnel-shaped structure 4 upper portion is equipped with the internal thread, 5 lower parts of cap are equipped with the external screw thread, funnel-shaped structure 4 and cap 5 and spiro union, 1 lower extreme inner wall of sampling bottle is equipped with the internal thread, 6 upper end outer walls of connecting seat are equipped with the external screw thread, sampling bottle 1 and 6 spiro unions of connecting seat, connecting hole 8 has been seted up to cap 5, connecting hole 8 at cap 5 top and connecting hole 3 below connecting seat 6 all are equipped with the silica gel sealing plug.
When the sampling bottle 1 of the utility model is used for sampling, the cap 5 and the funnel-shaped structure 4 are unscrewed, the endoscope tip is put into the funnel-shaped structure 4, the sampling liquid is directly collected, the junction of the funnel-shaped structure 4 and the connector 2 is unscrewed after returning to a laboratory, 1mL of liquid is absorbed by the injector to be inoculated on a flat plate, then the junction of the funnel-shaped structure 4 and the connector 2 of the auxiliary sampling component is screwed on, the silica gel sealing plug of the connecting hole 8 at the top of the cap 5 and the connecting hole 3 at the bottom of the connecting seat 6 is opened, the sampling bottle is put on the vacuum pump, the liquid is extracted by the vacuum pump, the connecting seat 6 at the lower part of the sampling bottle 1 is unscrewed, the filter membrane 7 is taken out, the filter membrane 7 is pasted on the flat plate and is put into a 36 ℃ incubator for cultivation, compared with the prior art, a longer sampling tube and a peristaltic pump are removed, the sampling liquid is directly collected by the sampling bottle during sampling, the peristaltic pump is not required, the sampling use is more convenient, meanwhile, a longer sampling tube is removed, the pollution chance can be reduced, and the funnel-shaped structure 4 and the joint of the connector 2 are directly unscrewed to extract 1mL of liquid more accurately and conveniently.
The above description is only for the preferred embodiment of the present invention, and for those skilled in the art, there are variations on the detailed description and the application scope according to the idea of the present invention, and the content of the description should not be construed as a limitation to the present invention.

Claims (6)

1. An endoscope detection sampling bottle is characterized in that: sampling bottle upper end is equipped with the connector, connector detachable is connected with supplementary sampling subassembly, the sampling bottle lower extreme is connected with the connecting seat that can open, connecting seat lower extreme middle part is equipped with the connecting hole that is used for connecting the vacuum pump, in the connecting seat and supplementary sampling subassembly all be equipped with 0.45 mu m's filter membrane.
2. An endoscopy sampling bottle according to claim 1, wherein: the auxiliary sampling assembly is arranged at the upper end edge of the sampling bottle and comprises a funnel-shaped structure connected with the connector, the upper end of the funnel-shaped structure is detachably connected with a cap, and a filter membrane of 0.45 mu m is arranged in the cap.
3. An endoscopy sampling bottle according to claim 2, wherein: the connecting hole has been seted up to the cap, the connecting hole at cap top and the connecting hole below the connecting seat all have the silica gel sealing plug.
4. An endoscopy sampling bottle according to claim 2, wherein: the connector inner wall is equipped with the internal thread, it is equipped with the external screw thread to leak hopper-shaped structure lower part, connector and hourglass hopper-shaped structure spiro union.
5. An endoscopy sampling bottle according to claim 2, wherein: funnel-shaped structure upper portion is equipped with the internal thread, the cap lower part is equipped with the external screw thread, funnel-shaped structure and cap spiro union.
6. An endoscopy sampling bottle according to claim 1, wherein: sampling bottle lower extreme inner wall is equipped with the internal thread, connecting seat upper end outer wall is equipped with the external screw thread, sampling bottle and connecting seat spiro union.
CN202121650902.1U 2021-07-20 2021-07-20 Scope detects sampling bottle Active CN215162658U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121650902.1U CN215162658U (en) 2021-07-20 2021-07-20 Scope detects sampling bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121650902.1U CN215162658U (en) 2021-07-20 2021-07-20 Scope detects sampling bottle

Publications (1)

Publication Number Publication Date
CN215162658U true CN215162658U (en) 2021-12-14

Family

ID=79392725

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121650902.1U Active CN215162658U (en) 2021-07-20 2021-07-20 Scope detects sampling bottle

Country Status (1)

Country Link
CN (1) CN215162658U (en)

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