CN215137107U

CN215137107U - Solid phase extraction device and system applied to animal tissue polyamine

Info

Publication number: CN215137107U
Application number: CN202121591598.8U
Authority: CN
Inventors: 姜冬梅; 王泽龙; 姜坤宏; 洪斌妍; 康波
Original assignee: Sichuan Agricultural University
Current assignee: Sichuan Agricultural University
Priority date: 2021-07-13
Filing date: 2021-07-13
Publication date: 2021-12-14
Anticipated expiration: 2031-07-13

Abstract

The utility model discloses a solid phase extraction device and system applied to animal tissue polyamine, which belongs to the technical field of solid phase extraction, and comprises a box body, wherein one side surface of the box body is an opening and closing surface, the upper surface of the box body is uniformly provided with a plurality of sample inlets with sealing rings, an inert gas inlet is arranged above the outer part of the box body, an air exhaust port is arranged below the outer part of the box body, a first bracket plate and a second bracket plate are fixed in the box body through chutes, a plurality of fixing holes with rubber rings are uniformly arranged on the first bracket plate and the second bracket plate, the fixing holes and the sample inlets are in one-to-one correspondence in the vertical direction, an extraction column and a collection bottle are respectively fixed on the first bracket plate and the second bracket plate through the fixing holes, and a height adjusting bracket is arranged below the collection bottle; the solid phase extraction system also comprises a gas circuit device and an extraction device, can effectively solve the problem that a sample is easy to be polluted in the prior art, and has the characteristics of simple structure and simple and convenient operation.

Description

Solid phase extraction device and system applied to animal tissue polyamine

Technical Field

The utility model relates to a solid phase extraction technical field, concretely relates to be applied to solid phase extraction system of animal tissue polyamine.

Background

The polyamines in the animal body mainly comprise putrescine, spermidine and spermine, and are low molecular aliphatic ammoniacal compounds with biological activity generated in the biological metabolism process. It is widely present in eukaryotic cells and prokaryotic cells, and plays a role in biological regulation in the processes of cell growth, tissue regeneration, inflammation repair and the like.

Solid phase extraction is a sample pretreatment technology, is mainly used for separation, purification and concentration of samples, and is widely applied to the fields of medicines, foods, environments, commercial inspection, chemical industry and the like. However, the conventional solid phase extraction devices are all exposed to the air for operation, which easily causes sample pollution and further affects the detection and analysis of the sample. Meanwhile, some animal tissues are easy to undergo reactions such as oxidation in the air, and thus the samples are deteriorated.

SUMMERY OF THE UTILITY MODEL

In view of the above disadvantages, the present invention provides a solid phase extraction device and system for animal tissue polyamine, which can effectively solve the problem of easy contamination of samples in the prior art, and has the characteristics of simple structure and easy operation.

In order to achieve the above object, the technical solution adopted by the present invention to solve the above problems is:

the utility model provides a be applied to solid-phase extraction device of animal tissue polyamine, the power distribution box comprises a box body, a side of box is the face of opening and shutting, the box upper surface evenly is provided with a plurality of introduction ports of taking the sealing washer, box outside top is provided with the inert gas air inlet, box outside below is provided with the extraction opening, box inside is fixed with mounting panel one and mounting panel two through the spout, evenly be provided with a plurality of fixed orificess of taking the rubber circle on mounting panel one and the mounting panel two, fixed orifices and introduction port one-to-one in vertical direction, mounting panel one and mounting panel two are fixed with extraction post and receiving flask respectively through the fixed orifices, the receiving flask below is provided with the altitude mixture control support.

The utility model has the advantages that: the utility model provides a solid phase extraction device applied to animal tissue polyamine, an inert gas inlet is arranged above the outer part of a box body, an air suction opening is arranged below the outer part of the box body, and the inert gas protection is provided for a sample in the solid phase extraction process; inert gas enters from the upper part of the box body, so that air is easily discharged from an outlet at the lower part, and the protection effect is improved; simultaneously, by arranging the height adjusting support, the height of the collecting bottle can be adjusted, and the problem of inconvenient operation caused by overlong or overlong collecting bottle is effectively solved.

Furthermore, a plurality of fixing grooves with rubber pads are uniformly formed in the upper surface of the height adjusting support, and the fixing grooves and the fixing holes are in one-to-one correspondence in the vertical direction.

The beneficial effect of adopting the further scheme is that: through the fixed slot of taking the rubber pad for the collecting flask is fixed more stable, and has effectively avoided the collecting flask bottom because of the broken problem of bumping with the support.

Furthermore, an observation window is arranged on the opening and closing surface.

The beneficial effect of adopting the further scheme is that: through the observation window, can observe the collection condition in the receiving flask constantly, make things convenient for the operator to carry out sample collection.

Furthermore, a pressure valve is arranged at the inert gas inlet, and a vacuum valve is arranged at the air exhaust port.

The beneficial effect of adopting the further scheme is that: the air pressure condition in the box body can be effectively controlled through the pressure valve and the vacuum valve; meanwhile, the air pressure condition in the box body can be changed according to the actual condition, and the extraction process of the extraction column can be accelerated when the air pressure condition is a proper negative pressure condition.

Further, the extraction column comprises a filtering layer, a packing layer and a tip part from top to bottom, and the height ratio of the filtering layer to the packing layer to the tip part is 1-2: 2-4: 1, preferably 1:4: 1.

The beneficial effect of adopting the further scheme is that: the separation and extraction effect of the extraction column can be ensured by arranging the filtering layer, the packing layer and the sharp mouth part and ensuring the proper height ratio; the arrangement of the beak part can accelerate the outflow of the extraction liquid and improve the extraction efficiency.

The utility model also provides a solid phase extraction system for animal tissue polyamine, including foretell solid phase extraction device.

Furthermore, the solid phase extraction system applied to animal tissue polyamine also comprises an air passage device and an extraction device.

The beneficial effect of adopting the further scheme is that: providing inert gas for the solid phase extraction device through the gas path device; the extraction of animal tissue polyamine is realized through the extraction device, so that the animal tissue polyamine enters the solid phase extraction device to be subjected to the next separation, purification, concentration and other processes, and an integrated treatment process is provided for the treatment of animal tissue polyamine samples.

Further, the gas circuit device comprises an inert gas tank and a vacuum pump, the inert gas tank is connected with an inert gas inlet in the solid-phase extraction device through a pipeline, and the vacuum pump is connected with an extraction opening of the solid-phase extraction device through a pipeline.

Further, the extraction device comprises a tank body, an outer wall layer, a temperature-variable layer and an inner wall layer are sequentially arranged in the tank body from outside to inside, a heat-conducting medium inlet and a heat-conducting medium outlet are arranged above the temperature-variable layer, a pipeline with two ends respectively connected with the heat-conducting medium inlet and the heat-conducting medium outlet is arranged inside the temperature-variable layer, a raw material inlet, an extracting solution inlet and a stirring motor are arranged above the inner wall layer, the stirring motor is connected with a homogenizing rod through a stirring shaft, and a discharge port is arranged at the lower part of the tank body.

The beneficial effect of adopting the further scheme is that: a controllable temperature change is provided for the tank body through a heat-conducting medium in the temperature-changing layer, so that the temperature requirement in the process of extracting polyamine from animal tissues is met; for example, when animal tissues are homogenized, the heat-conducting medium is low-temperature fluid, so that a low-temperature environment is provided for the homogenization process; after homogenizing, adding the extracting solution, replacing the heat-conducting medium with high-temperature fluid, increasing the temperature in the tank body, and improving the extraction rate of polyamine in animal tissues.

Further, the inside of the outer wall layer is an air heat insulation layer.

The beneficial effect of adopting the further scheme is that: through the air heat insulation layer, the heat loss is reduced, and the effect of the temperature change layer is ensured.

Further, the inside on inner wall layer is provided with temperature sensor and level sensor, and the external portion of jar is provided with the controller, electric connection between temperature sensor, level sensor and the controller.

The beneficial effect of adopting the further scheme is that: through being provided with temperature sensor, level sensor and controller, can effectively control the temperature in the jar body and the water level situation of change.

Drawings

FIG. 1 is a schematic structural diagram of a solid phase extraction device for animal tissue polyamines according to the present invention;

FIG. 2 is a schematic structural view of an extraction column of the present invention;

FIG. 3 is a schematic structural view of a middle supporting plate of the present invention;

fig. 4 is a schematic structural view of a height adjusting bracket of the present invention;

FIG. 5 is a schematic structural view of the extraction device of the present invention;

wherein, 1, a box body; 2. opening and closing the surface; 3. a sample inlet; 4. an inert gas inlet; 5. an air extraction opening; 6. a chute; 7. a first support plate; 8. a second bracket plate; 9. a fixing hole; 10. an extraction column; 11. a collection bottle; 12. a height adjustment bracket; 13. fixing grooves; 14. an observation window; 15. a pressure valve; 16. a filter layer; 17. a filler layer; 18. a nib part; 19. a tank body; 20. an outer wall layer; 21. a temperature-changing layer; 22. an inner wall layer; 23. a heat transfer medium inlet; 24. a heat transfer medium outlet; 25. a pipeline; 26. a raw material inlet; 27. an extracting solution inlet; 28. a stirring motor; 29. a temperature sensor; 30. a water level sensor; 31. a vacuum valve; 32. a controller; 33. homogenizing the bar; 34. and (4) a discharge port.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings.

In one embodiment of the present invention, as shown in fig. 1-5, a solid phase extraction apparatus applied to animal tissue polyamine is provided, which comprises a box body 1, a side surface of the box body 1 is an opening and closing surface 2, an observation window 14 is arranged on the opening and closing surface 2, a plurality of sample inlets 3 with sealing rings are uniformly arranged on the upper surface of the box body 1, an inert gas inlet 4 is arranged above the outer portion of the box body 1, an air exhaust opening 5 is arranged below the outer portion of the box body 1, a pressure valve 15 is arranged at the position of the inert gas inlet 4, a vacuum valve 31 is arranged at the position of the air exhaust opening 5, a first support plate 7 and a second support plate 8 are fixed inside the box body 1 through a chute 6, a plurality of fixing holes 9 with rubber rings are uniformly arranged on the first support plate 7 and the second support plate 8, the fixing holes 9 are in one-to-one correspondence with the sample inlets 3 in the vertical direction, an extraction column 10 and a collection bottle 11 are respectively fixed on the first support plate 7 and the second support plate 8 through the fixing holes 9, the extraction column 10 comprises a filtering layer 16, a packing layer 17 and a sharp mouth part 18 from top to bottom, the height ratio of the filtering layer 16 to the packing layer 17 to the sharp mouth part 18 is 1:4:1, a height adjusting support 12 is arranged below the collection bottle 11, a plurality of fixing grooves 13 with rubber pads are uniformly arranged on the upper surface of the height adjusting support 12, and the fixing grooves 13 and the fixing holes 9 are in one-to-one correspondence in the vertical direction; the utility model provides a be applied to animal tissue polyamine's solid phase extraction device, box 1 outside top is provided with inert gas air inlet 4, box 1 outside below is provided with extraction opening 5, for the sample provides the inert gas protection in the solid phase extraction process, the extraction finishes, can be under the condition of continuously letting in inert gas, seals collecting bottle 11, has effectively avoided the animal tissue active material such as polyamine easily to be air pollution and the problem that takes place the oxidation rotten; inert gas enters from the upper part of the box body 1, so that air is easily discharged from an outlet at the lower part, and the protection effect is improved; simultaneously, by arranging the height adjusting bracket 12, the height of the collecting bottle 11 can be adjusted, and the problem of inconvenient operation caused by overlong or overlong collecting bottle 11 is effectively avoided.

The embodiment provides a solid phase extraction system applied to animal tissue polyamine, which comprises the solid phase extraction device, an air channel device and an extraction device, wherein the air channel device comprises an inert gas tank and a vacuum pump, the inert gas tank is connected with an inert gas inlet 4 of the solid phase extraction device through a pipeline, and the vacuum pump is connected with an extraction opening 5 of the solid phase extraction device through a pipeline; the extraction device comprises a tank body 19, an outer wall layer 20, a temperature-changing layer 21 and an inner wall layer 22 are sequentially arranged in the tank body 19 from outside to inside, an air heat insulation layer is arranged inside the outer wall layer 20, a heat-conducting medium inlet 23 and a heat-conducting medium outlet 24 are arranged above the temperature-changing layer 21, a pipeline 25 with two ends respectively connected with the heat-conducting medium inlet 23 and the heat-conducting medium outlet 24 is arranged inside the temperature-changing layer 21, a raw material inlet 26 is arranged above the inner wall layer 22, the extracting solution inlet 27 and the stirring motor 28, the stirring motor 28 is connected with the homogenizing rod 33 through a stirring shaft, the lower part of the tank body 19 is provided with a discharge port 34, the inside of the inner wall layer 22 is provided with a temperature sensor 29 (the specific model is DS18B20) and a water level sensor 30 (the specific model is JRWL0524-03), the outside of the tank body 19 is provided with a controller 32 (the specific model is ohm dragon CP1H-X40DT-D type PLC), and the temperature sensor 29, the water level sensor 30 and the controller 32 are electrically connected. The gas path device provides inert gas for the solid phase extraction device; the extraction of animal tissue polyamine is realized through the extraction device, so that the animal tissue polyamine enters the solid phase extraction device to be subjected to the next separation, purification, concentration and other processes, and an integrated treatment process is provided for the treatment of animal tissue polyamine samples.

When in use, animal tissues are added into the tank body 19 of the extraction device from the raw material inlet 26, low-temperature fluid is introduced into the pipeline 25 of the temperature-changing layer 21, the stirring motor 28 is started, and homogenate is carried out for 1 hour at low temperature; then adding the extracting solution from an extracting solution inlet 27, introducing high-temperature fluid into a pipeline 25 of the temperature-changing layer 21, maintaining the temperature within the range of 50-65 ℃ through a controller 32, and stirring and extracting for 30 min; and then transferring the extracting solution to a solid phase extraction device for extraction operation, and completing the whole treatment process of obtaining the polyamine from the animal tissue sample.

While the present invention has been described in detail and with reference to the accompanying drawings, it is not to be considered as limited to the scope of the invention. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims

1. The solid phase extraction device applied to animal tissue polyamine is characterized by comprising a box body (1), wherein one side surface of the box body (1) is an opening and closing surface (2), a plurality of sample inlets (3) with sealing rings are uniformly arranged on the upper surface of the box body (1), an inert gas inlet (4) is arranged above the outer part of the box body (1), an air suction opening (5) is arranged below the outer part of the box body (1), a first bracket plate (7) and a second bracket plate (8) are fixed in the box body (1) through sliding grooves (6), a plurality of fixing holes (9) with rubber rings are uniformly arranged on the first bracket plate (7) and the second bracket plate (8), the fixing holes (9) are in one-to-one correspondence with the sample inlets (3) in the vertical direction, an extraction column (10) and a collection bottle (11) are respectively fixed on the first bracket plate (7) and the second bracket plate (8) through the fixing holes (9), a height adjusting bracket (12) is arranged below the collecting bottle (11).

2. The solid phase extraction device applied to animal tissue polyamine of claim 1 wherein the upper surface of the height adjusting bracket (12) is uniformly provided with a plurality of fixing grooves (13) with rubber pads, and the fixing grooves (13) and the fixing holes (9) are in one-to-one correspondence in the vertical direction.

3. The solid phase extraction device applied to animal tissue polyamine of claim 1 wherein the opening and closing face (2) is provided with an observation window (14).

4. The solid phase extraction device applied to animal tissue polyamine of claim 1 wherein the inert gas inlet (4) is provided with a pressure valve (15) and the suction port (5) is provided with a vacuum valve (31).

5. The solid phase extraction device applied to animal tissue polyamines as claimed in claim 1, wherein the extraction column (10) comprises, from top to bottom, a filter layer (16), a filler layer (17) and a tip (18).

6. The solid phase extraction device applied to animal tissue polyamine of claim 5, wherein the height ratio of the filter layer (16), the filler layer (17) and the sharp mouth part (18) is 1-2: 2-4: 1.

7. A solid phase extraction system for use with polyamines from animal tissue comprising the solid phase extraction device of any one of claims 1 to 6.

8. The solid phase extraction system for polyamines from animal tissue of claim 7 further comprising a gas circuit means and an extraction means.

9. The solid-phase extraction system applied to animal tissue polyamine of claim 8, wherein the gas circuit device comprises an inert gas tank and a vacuum pump, the inert gas tank is connected with an inert gas inlet (4) of the solid-phase extraction device through a pipeline, and the vacuum pump is connected with a pumping port (5) of the solid-phase extraction device through a pipeline.

10. The solid-phase extraction system applied to animal tissue polyamine of claim 8, wherein the extraction device comprises a tank (19), the tank (19) is sequentially provided with an outer wall layer (20), a temperature-changing layer (21) and an inner wall layer (22) from outside to inside, a heat-conducting medium inlet (23) and a heat-conducting medium outlet (24) are arranged above the temperature-changing layer (21), a pipeline (25) with two ends respectively connected with the heat-conducting medium inlet (23) and the heat-conducting medium outlet (24) is arranged inside the temperature-changing layer (21), a raw material inlet (26), an extract inlet (27) and a stirring motor (28) are arranged above the inner wall layer (22), the stirring motor (28) is connected with a homogenizing rod (33) through a stirring shaft, and a discharge hole (34) is arranged at the lower part of the tank (19).