CN215050375U - Anaerobic cell culture device - Google Patents
Anaerobic cell culture device Download PDFInfo
- Publication number
- CN215050375U CN215050375U CN202120512840.1U CN202120512840U CN215050375U CN 215050375 U CN215050375 U CN 215050375U CN 202120512840 U CN202120512840 U CN 202120512840U CN 215050375 U CN215050375 U CN 215050375U
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- CN
- China
- Prior art keywords
- culture
- culture box
- box
- cell culture
- oxygen
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 45
- 239000001301 oxygen Substances 0.000 claims abstract description 45
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 45
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 16
- 230000001146 hypoxic effect Effects 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 238000004891 communication Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 abstract description 10
- 239000007789 gas Substances 0.000 description 3
- 208000003251 Pruritus Diseases 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an oxygen lack cell culture device belongs to biological cultivation technical field. An apparatus for culturing hypoxic cells, comprising: an oxygen tank and a culture box; the culture box is respectively communicated with an air inlet pipe and an exhaust pipe, the air inlet pipe is also communicated with an oxygen tank, a bracket is arranged in the culture box, and a movable door plate is arranged on the side wall of the culture box; the support includes pivot and multilayer backup pad, and the backup pad is connected with the lateral wall of pivot respectively, is equipped with the standing groove in the backup pad respectively. The culture box of the utility model is connected with an oxygen tank for oxygen supply through an air inlet pipe, and an exhaust pipe is used for communicating with the air; the inside of culture box is equipped with the support to connect the multilayer backup pad through the lateral wall of pivot, put the cell culture dish and carry out cell culture in the standing groove of backup pad, through opening the rotatory pivot of movable door board, thereby be convenient for take the culture dish of different positions in the culture process.
Description
Technical Field
The utility model relates to a biological cultivation technical field, concretely relates to hypoxia cell culture device.
Background
The anaerobic cell refers to cells and tissues with oxygen supply quantity which can not fully meet the metabolic demand, and the oxygen supply is needed to be externally connected in the process of cultivating the anaerobic cell to ensure the normal growth and metabolism. In the process of culturing the hypoxic cells, the culture of the hypoxic cells in individual culture dishes is failed because the culture dishes are distributed too densely, and a larger culture space must be ensured when the hypoxic cell culture dishes are arranged at intervals. The conventional laboratory culture conditions are relatively limited, and the conventional culture dishes are overlapped, so that the culture dishes at the bottom are taken out, which is troublesome. Therefore, the prior art has the problem of difficult taking in the process of culturing the hypoxic cells.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an oxygen lack cell culture device to solve the problem of the difficulty of taking among the prior art oxygen lack cell culture process.
The utility model provides an above-mentioned technical problem's technical scheme as follows:
an apparatus for culturing hypoxic cells, comprising: an oxygen tank and a culture box;
the culture box is respectively communicated with an air inlet pipe and an exhaust pipe, the air inlet pipe is also communicated with an oxygen tank, a bracket is arranged in the culture box, and a movable door plate is arranged on the side wall of the culture box; the support includes pivot and multilayer backup pad, pivot and the inner wall normal running fit who cultivates the box, and the backup pad is connected with the lateral wall of pivot respectively, is equipped with the standing groove in the backup pad respectively.
The culture box of the utility model is connected with an oxygen tank for oxygen supply through an air inlet pipe, and an exhaust pipe is used for communicating with the air; the inside of culture box is equipped with the support to connect the multilayer backup pad through the lateral wall of pivot, put the cell culture dish and carry out cell culture in the standing groove of backup pad, through opening the rotatory pivot of movable door board, thereby be convenient for take the culture dish of different positions in the culture process.
Furthermore, the top and the bottom of the rotating shaft are respectively connected with the inner wall of the culture box.
The utility model discloses a pivot both ends are fixed to rotate the in-process and make whole support can remain stable, avoid because the culture dish is placed inhomogeneously and cause the pivot slope.
Further, the rotating shaft is conical.
The rotating shaft of the utility model is arranged in a cone shape to facilitate the air flow in the culture box, and avoid the over-low oxygen content near the culture dish close to the side wall at the bottom of the rotating shaft, thereby influencing the growth of anaerobic cells in the culture dish; and the conical outer wall surface of the rotating shaft can facilitate the annular arrangement of more supporting plates.
Furthermore, a control chip and an oxygen sensor in communication connection with the control chip are arranged on the inner side of the culture box.
The utility model discloses the inboard oxygen sensor of incubator is used for detecting the concentration of itching that contains of incubator internal gas.
Furthermore, the outer side of the culture box is provided with a display screen which is in communication connection with the control chip.
The utility model discloses a display screen is used for showing the oxygen concentration that oxygen sensor detected.
Furthermore, a loudspeaker which is connected with the control chip in a communication way is arranged on the outer side of the culture box.
The utility model discloses when oxygen content is not in the allowed band in the culture box, the speaker sound production is reminded.
Furthermore, the air inlet pipe and the exhaust pipe are respectively provided with an electromagnetic valve, and the electromagnetic valves are in communication connection with the control chip.
The utility model discloses set up the solenoid valve in intake pipe and blast pipe to with control chip's intercommunication, can be according to the break-make state of oxygen content intelligent control solenoid valve in cultivateing the box, thereby make the oxygen concentration in cultivateing the box remain throughout in suitable within range.
The utility model discloses following beneficial effect has:
(1) the culture box of the utility model is connected with an oxygen tank for oxygen supply through an air inlet pipe, and an exhaust pipe is used for communicating with the air; the inside of culture box is equipped with the support to connect the multilayer backup pad through the lateral wall of pivot, put the cell culture dish and carry out cell culture in the standing groove of backup pad, through opening the rotatory pivot of movable door board, thereby be convenient for take the culture dish of different positions in the culture process.
(2) The utility model discloses a pivot both ends are fixed to guarantee at the rotation in-process that whole support can be stable, avoid causing the pivot slope because the culture dish is placed inhomogeneously.
(3) The rotating shaft of the utility model is arranged in a cone shape to facilitate the air flow in the culture box, and avoid the over-low oxygen content near the culture dish close to the side wall at the bottom of the rotating shaft, thereby influencing the growth of anaerobic cells in the culture dish; and the conical outer wall surface of the rotating shaft can facilitate the annular arrangement of more supporting plates.
Drawings
FIG. 1 is a schematic structural view of the anaerobic cell culture device of the present invention;
FIG. 2 is a cross-sectional view of the anaerobic cell culture device of the present invention.
In the figure: 10-an oxygen tank; 20-culture box; 21-an air inlet pipe; 22-an exhaust pipe; 23-a scaffold; 24-a rotating shaft; 25-a support plate; 26-placing a groove; 29-an oxygen sensor; 30-a display screen; 31-a loudspeaker; 32-solenoid valves; 33-movable door panel.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
Examples
Referring to fig. 1, a hypoxic cell culture apparatus, comprising: an oxygen tank 10 and an incubation box 20.
The top of the culture box 20 is respectively communicated with an air inlet pipe 21 and an air outlet pipe 22, the air inlet pipe 21 is communicated with the oxygen tank 10 to supply oxygen to the interior of the culture box 20, and the air outlet pipe 22 is communicated with the outside air to discharge the gas in the culture box 20.
Referring to fig. 1 and 2, the incubation box 20 is further provided with a control chip, which is a single chip microcomputer of model STM32F407ZGT6-LQFP144, for writing a control program, receiving and sending out electric signals, and converting the electric signals into digital signals and the like. An oxygen sensor 29 is connected to the side wall of the culture box 20 and is in communication with the control chip, and the oxygen sensor 29 is used for detecting the oxygen content in the gas inside the culture box 20, so that the oxygen supply rate of the oxygen tank 10 can be conveniently adjusted. The display screen 30 is arranged on the outer side of the culture box 20, the display screen 30 is also in communication connection with the control chip, and the oxygen concentration in the culture box 20 measured by the oxygen sensor 29 can be displayed, so that the staff can conveniently observe and refer to the oxygen concentration. The outside of cultivateing box 20 still is equipped with speaker 31, speaker 31 and control chip communication connection, and when the inside oxygen concentration of cultivateing box 20 is not in suitable range, control chip sends the signal of telecommunication, is sent by the sound wave of speaker 31 conversion to remind relevant staff to adjust.
The air inlet pipe 21 and the air outlet pipe 22 are respectively provided with an electromagnetic valve 32, the electromagnetic valves 32 are in communication connection with a control chip, and the control chip can intelligently control the on-off state of the two electromagnetic valves 32 according to the oxygen content in the culture box 20, so that the oxygen concentration in the culture box 20 is always kept in a proper range.
The inside of culture box 20 is equipped with support 23, and support 23 includes pivot 24 and multilayer backup pad 25, and pivot 24 and the inner wall normal running fit of culture box 20 are equipped with standing groove 26 on the backup pad 25 respectively for place the hypoxia cell culture dish. The top and the bottom of pivot 24 are connected with the inner wall of culture box 20 respectively to be equipped with the supporting seat in the position of connecting, inject through the pivot 24 both ends, thereby make whole support 23 can remain stable at the rotation in-process, avoid placing inhomogeneous and cause the pivot 24 slope because of the culture dish. The shape of the rotating shaft 24 is a cone with a small upper part and a big lower part, which is convenient for the air in the culture box 20 to flow and avoids the phenomenon that the oxygen content near the culture dish close to the side wall of the bottom of the rotating shaft 24 is too low, thereby influencing the growth of anoxic cells in the culture dish; and more support plates 25 can be conveniently and annularly arranged through the conical outer wall surface of the rotating shaft 24.
The culture box 20 is provided with a movable door plate 33, and the movable door plate 33 is in sealing fit with the culture box 20. When the culture dish needs to be taken out, the movable door plate 33 is opened, the bracket 23 is moved to the position of the movable door plate 33, and the rotating shaft 24 is rotated to a proper position, so that the culture dish at any position can be taken out conveniently.
The utility model discloses an operation process: (1) opening the movable door plate 33, and placing the culture dish filled with the anaerobic cells in the placing groove 26; (2) closing the movable door plate 33 and starting to culture the anaerobic cells; (3) observing the itch-containing concentration detected by the oxygen sensor 29 through the display screen 30; (4) the movable door plate 33 is opened, the rotating shaft 24 is rotated, and the culture dishes at different positions are taken out for culture state analysis.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (7)
1. An apparatus for culturing hypoxic cells, comprising: an oxygen tank (10) and a culture box (20);
the culture box (20) is respectively communicated with an air inlet pipe (21) and an air outlet pipe (22), the air inlet pipe (21) is also communicated with the oxygen tank (10), a support (23) is arranged inside the culture box (20), and a movable door plate (33) is arranged on the side wall of the culture box (20);
the support (23) comprises a rotating shaft (24) and a plurality of layers of supporting plates (25), the rotating shaft (24) is in running fit with the inner wall of the culture box (20), the supporting plates (25) are respectively connected with the side walls of the rotating shaft (24), and the supporting plates (25) are respectively provided with a placing groove (26).
2. The hypoxic cell culture device according to claim 1, wherein the top and bottom of the rotating shaft (24) are connected with the inner wall of the culture box (20), respectively.
3. The hypoxic cell culture device according to claim 2, wherein the rotating shaft (24) is tapered.
4. The hypoxic cell culture device according to any one of claims 1 to 3, wherein the inside of the culture cassette (20) is provided with a control chip and an oxygen sensor (29) in communication connection with the control chip.
5. The hypoxic cell culture device according to claim 4, wherein the outer side of the culture box (20) is provided with a display screen (30) which is in communication connection with the control chip.
6. The hypoxic cell culture device according to claim 5, wherein the outer side of the culture box (20) is provided with a loudspeaker (31) which is in communication connection with the control chip.
7. The hypoxic cell culture device according to claim 6, wherein the air inlet pipe (21) and the air outlet pipe (22) are respectively provided with an electromagnetic valve (32), and the electromagnetic valves (32) are in communication connection with the control chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120512840.1U CN215050375U (en) | 2021-03-10 | 2021-03-10 | Anaerobic cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120512840.1U CN215050375U (en) | 2021-03-10 | 2021-03-10 | Anaerobic cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215050375U true CN215050375U (en) | 2021-12-07 |
Family
ID=79260403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120512840.1U Expired - Fee Related CN215050375U (en) | 2021-03-10 | 2021-03-10 | Anaerobic cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215050375U (en) |
-
2021
- 2021-03-10 CN CN202120512840.1U patent/CN215050375U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211207 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |