CN214991515U

CN214991515U - Device for rapidly extracting dental pulp stem cells

Info

Publication number: CN214991515U
Application number: CN202120198710.5U
Authority: CN
Inventors: 王馨雨
Original assignee: Youya Biotechnology Shanghai Co ltd
Current assignee: Youya Biotechnology Shanghai Co ltd
Priority date: 2021-01-25
Filing date: 2021-01-25
Publication date: 2021-12-03
Anticipated expiration: 2031-01-25

Abstract

The utility model discloses a draw device of pulp stem cell fast, which comprises a housin, the casing top is provided with the cap, casing bottom and push rod motor fixed connection, the inside reaction tank that is provided with of casing, reaction tank bottom surface and shells inner wall fixed connection form sealed space, shells inner wall fixedly connected with filter screen, the filter screen bottom is equipped with the separation tank, the inside sump that is equipped with of casing, it is equipped with the ball to go out the sump top surface, the separation tank passes through ball swing joint with a sump, the cap both sides are equipped with inlet tube and stifled round pin respectively, it has the agitator to run through in the cap, the agitator bottom is placed in the reaction tank, the inlet tube is provided with the valve. The utility model discloses a set up regions such as reaction tank, separation tank and play sump in the casing, integrate different step equipment at a device, whole process need not shift, reduces the contact with the air, reduces the infection risk, improves extraction efficiency.

Description

Device for rapidly extracting dental pulp stem cells

Technical Field

The utility model relates to a medical apparatus device, in particular to a device for rapidly extracting dental pulp stem cells.

Background

The pulp tissue is located within the pulp chamber inside the tooth and is the only soft tissue in the tooth tissue. Fibroblasts isolated from dental pulp tissue are called dental pulp stem cells. The cell has immunophenotype and mineralization nodule forming ability very similar to bone marrow mesenchymal stem cell, is fusiform in cell form, can self-renew and differentiate in multiple directions, and has strong cloning ability. The dental pulp stem cell has the potential of multidirectional differentiation, and can be differentiated into cell line types such as fat, bone, cartilage, muscle, vascular endothelium, liver, nerve and the like through the induction of different cytokines besides the cell capable of forming mineralized nodules. The dental pulp stem cells have the advantages of rich sources. The natural deciduous teeth of children 6-11 years old and wisdom teeth that adults need to pull out all contain abundant dental pulp stem cells; has less side effect. The dental pulp stem cell is a mesenchymal stem cell, has the universality of the mesenchymal stem cell, has low immunogenicity, can be used without strict pairing, does not cause strong rejection reaction, and has an immunoregulation function, and foreign bodies can not cause rejection reaction all the time; there is no ethical dispute. The dental pulp stem cells are obtained from naturally-exfoliated teeth of children and wisdom teeth of adults, belong to waste articles, do not damage life, and do not have ethical disputes.

In the extraction work of the dental pulp stem cells, different devices are respectively used in each step to carry out the multiple transfer, the contact frequency with air is increased, the risk of bacterial pollution and the like is caused, and in order to solve the problems and simplify the operation process, the device for quickly extracting the dental pulp stem cells is provided.

SUMMERY OF THE UTILITY MODEL

This use neotype aim at provides a draw device of pulp stem cell fast, through set up reaction tank, separation tank and play sump etc. in the device casing, can avoid pulp stem cell to draw in-process different steps and use different devices respectively to carry out the hidden danger that needs carry out many times to shift and cause the pollution, improves extraction efficiency.

The purpose of the utility model can be realized by the following technical scheme:

the utility model provides a draw device of pulp stem cell fast, includes the casing, the casing top is provided with the cap, casing bottom and push rod motor fixed connection, the inside reaction tank that is provided with of casing, reaction tank bottom surface and shells inner wall fixed connection form confined space, shells inner wall fixedly connected with filter screen, the filter screen bottom is equipped with the separation tank, the inside sump that is equipped with of casing, the sump top surface that goes out is equipped with the ball, the separation tank passes through ball swing joint with the sump that goes out.

The reactor is characterized in that a water inlet pipe and a blocking pin are respectively arranged on two sides of the shell cover, a stirrer penetrates through the shell cover, and the bottom of the stirrer is arranged in the reaction tank.

The inside stifled round pin that is provided with of reaction tank, stifled round pin both ends run through cap and reaction tank bottom respectively.

The inside carousel that is provided with of casing, motor output shaft end fixed connection is passed through to the carousel bottom, separation tank bottom is run through on carousel upper portion, the separation tank bottom surface is provided with the spout, the clearance has been left with the casing to the separation tank side, and the reciprocating of accessible casing realizes forming confined space with the carousel.

A water outlet is arranged below the water outlet bin, the water outlet is connected with the culture dish in a sealing mode through a hose, and the hose is provided with a first valve.

The two ends of the push rod motor are fixedly connected with the shell and the workbench respectively and have a telescopic function, and the position of the shell can be adjusted to move.

The water inlet pipe is provided with a second valve.

A plurality of grooves are fixed at the top of the water outlet bin, and balls are movably connected in the grooves.

And an extraction pipeline is arranged on one side of the separation tank.

The utility model has the advantages that: the plug pin penetrates through the shell cover and the bottom of the reaction tank, the depth of the plug pin is adjusted, when dental pulp tissues and enzyme reaction liquid are mixed, the plug pin is inserted to form a sealed reaction area, after the reaction is completed, the plug pin is pulled out, so that the reaction liquid flows into the next step, liquid transfer can be effectively carried out, and the contact with the outside air is avoided.

Through adjusting the push rod motor, adjustment casing position drives the separation tank bottom and forms sealed space with the carousel, and it is rotatory that the motor drives the carousel, and the transmission is rotatory at the same time the separation tank, separates the operation, realizes the liquid transfer in the same device, simplifies and draws the flow.

Through setting up reaction tank, separation tank and regions such as play sump in the casing, integrate different step equipment at a device, whole process need not shift, reduces the contact with the air, reduces the infection risk, improves extraction efficiency.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

FIG. 1 is an overall structure of the present invention;

FIG. 2 is a detail view of the separation cell and carousel;

FIG. 3 is a detail view of a chute at the bottom of the separation tank;

FIG. 4 is a detailed view of the balls at the top of the outlet bin.

In the figure: 1. a housing; 2. a shell cover; 3. a reaction tank; 4. filtering with a screen; 5. a separation tank; 6. a turntable; 7. a water outlet bin; 8. a push rod motor; 9. a stirrer; 10. blocking pins; 11. a hose; 12. a first valve; 13. a culture dish; 14. a water inlet pipe; 15. a ball bearing; 16. an extraction line; 17. a second valve; 18. a chute; 19. and (4) a groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

Referring to fig. 1-4, a device for rapidly extracting dental pulp stem cells includes a housing 1, a housing cover 2 is disposed on the top of the housing 1, the bottom of the housing 1 is fixedly connected to a push rod motor 8, a reaction tank 3 is disposed inside the housing 1, the bottom of the reaction tank 3 is fixedly connected to the inner wall of the housing 1 to form a sealed space, a filter screen 4 is fixedly connected to the inner wall of the housing 1, a separation tank 5 is disposed at the bottom of the filter screen 4, a water outlet bin 7 is disposed inside the housing 1, a ball 15 is disposed on the top surface of the water outlet bin 7, and the separation tank 5 is movably connected to the water outlet bin 7 through the ball 15.

A water inlet pipe 14 and a blocking pin 10 are respectively arranged on two sides of the shell cover 2, a stirrer 9 penetrates through the shell cover 2, and the bottom of the stirrer 9 is arranged in the reaction tank 3.

The reaction tank 3 is internally provided with a blocking pin 10, and two ends of the blocking pin 10 respectively penetrate through the bottom of the shell cover 2 and the bottom of the reaction tank 3.

The inside carousel 6 that is provided with of casing 1, motor output shaft end fixed connection is passed through to 6 bottoms of carousel, 5 bottoms in separation tank are run through on 6 upper portions of carousel, 5 bottom surfaces in separation tank are provided with

spout

18, 5 sides in separation tank leave the clearance with casing 1, and reciprocating of accessible casing 1 realizes forming confined space with carousel 6,

carousel

6 and 5 lateral walls in separation tank are equipped with the draw-in groove respectively, through adjusting the motion of casing 1, can realize connecting and separating.

A water outlet is arranged below the water outlet bin 7 and is in sealing connection with a culture dish 13 through a hose 11, and the hose 11 is provided with a first valve 12.

Two ends of the push rod motor 8 are fixedly connected with the shell 1 and the workbench respectively and have a telescopic function, and the position of the shell 1 can be adjusted to move.

The inlet pipe 14 is provided with a second valve 17.

A plurality of grooves 19 are fixed on the top of the water outlet bin 7, and balls 15 are movably connected in the grooves 19.

An extraction pipeline 16 is arranged on one side of the separation tank 5.

The utility model discloses a theory of operation: placing the obtained dental pulp tissue in a reaction tank 3, closing a shell cover 2 for sealing, injecting enzyme reaction liquid through a water inlet pipe 14, performing light and uniform stirring by using a stirrer 9, fully mixing the dental pulp tissue and the enzyme reaction liquid, adjusting a push rod motor 8 after the reaction is finished, driving a shell 1 to move upwards, closing the bottom of a separation tank 5 and a clamping groove of a rotary disc 6, pulling a stop pin 10 upwards, pulling the stop pin 10 out of a water outlet hole at the bottom of the reaction tank 3, enabling the mixed liquid to flow out of the water outlet hole, filtering the mixed liquid by a filter screen 4, entering the separation tank 5, driving the rotary disc 6 and the separation tank 5 to rotate by the motor, separating the mixed liquid to obtain layered liquid, extracting supernatant through an extraction pipeline 16, adjusting the push rod motor 8 after the extraction is finished, enabling the shell 1 to move downwards, separating the rotary disc 6 from the separation tank 5, enabling the obtained dry cell suspension to flow into a water outlet 7 from the bottom of the separation tank 5, the dental pulp stem cells are extracted by entering a culture dish 13 through a hose 11. The whole-course extraction device is in a sealed state and does not need to be in contact with air.

The invention is limited only by the claims and the full scope and equivalents thereof, and further, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

The foregoing is merely exemplary and illustrative of the structure of the invention, and various modifications, additions and substitutions as described in the detailed description may be made by those skilled in the art without departing from the structure or exceeding the scope of the invention as defined in the claims.

Claims

1. The utility model provides a draw device of pulp stem cell fast, a serial communication port, includes casing (1), casing (1) top is provided with cap (2), casing (1) bottom and push rod motor (8) fixed connection, casing (1) inside is provided with reaction tank (3), reaction tank (3) bottom surface and casing (1) inner wall fixed connection form seal space, casing (1) inner wall fixedly connected with filter screen (4), filter screen (4) bottom is equipped with separation tank (5), casing (1) inside is equipped with out sump (7), it is equipped with ball (15) to go out sump (7) top surface, separation tank (5) and play sump (7) are through ball (15) swing joint.

2. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein a water inlet pipe (14) is arranged at the top of the shell cover (2), a stirrer (9) penetrates through the shell cover (2), and the bottom of the stirrer (9) is arranged in the reaction tank (3).

3. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein the reaction cell (3) is internally provided with a blocking pin (10), and two ends of the blocking pin (10) respectively penetrate through the shell cover (2) and the side wall of the reaction cell (3).

4. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein a rotary disc (6) is arranged inside the housing (1), the bottom of the rotary disc (6) is fixedly connected with the tail end of the output shaft of the motor, the upper part of the rotary disc (6) penetrates through the bottom of the separation tank (5), the bottom of the separation tank (5) is provided with a sliding chute (18), and the side surface of the separation tank (5) is provided with a movable gap with the housing (1).

5. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein a water outlet is arranged below the water outlet bin (7), the water outlet is hermetically connected with a culture dish (13) through a hose (11), and the hose (11) is provided with a first valve (12).

6. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein the two ends of the push rod motor (8) are fixedly connected with the shell (1) and the workbench respectively.

7. The device for rapid extraction of dental pulp stem cells according to claim 2, wherein the water inlet tube (14) is provided with a second valve (17).

8. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein a plurality of grooves (19) are fixed on the top of the water outlet bin (7), and balls (15) are movably connected in the grooves (19).

9. The device for rapidly extracting dental pulp stem cells according to claim 1, wherein an extraction pipeline (16) is arranged at one side of the separation tank (5).