CN214991474U

CN214991474U - Separation and concentration device for stem cell exosomes

Info

Publication number: CN214991474U
Application number: CN202120212502.6U
Authority: CN
Inventors: 王馨雨
Original assignee: Youya Biotechnology Shanghai Co ltd
Current assignee: Youya Biotechnology Shanghai Co ltd
Priority date: 2021-01-26
Filing date: 2021-01-26
Publication date: 2021-12-03
Anticipated expiration: 2031-01-26

Abstract

The utility model discloses a separation and concentration device of stem cell exosomes, which comprises a bottom plate, an outer cylinder and a separation cylinder; the top surface of the bottom plate is symmetrically provided with support rods, two sides of the outer cylinder are installed on the support rods through fixing blocks, the separating cylinder is positioned in the outer cylinder, the side wall of the separating cylinder is connected with the outer cylinder through a limiting part, and the bottom of the separating cylinder is connected with the rotating shaft through a connecting piece; the separating cylinder comprises a top ring, a bottom ring, a first reinforcing rib, a first filter screen, a second reinforcing rib, a second filter screen and a central plate; the top ring is positioned right above the bottom ring, a plurality of groups of first reinforcing ribs are arranged in an annular array, the top ends of the first reinforcing ribs are connected with the top ring, the bottom ends of the first reinforcing ribs are connected with the bottom ring, and the first filter screen is arranged among the top ring, the bottom ring and the first reinforcing ribs; the utility model discloses a separation enrichment facility has and conveniently installs dismantlement and cleaning work advantage.

Description

Separation and concentration device for stem cell exosomes

Technical Field

The utility model belongs to the technical field of the stem cell exosome, a separation enrichment facility is related to, specifically is a separation enrichment facility of stem cell exosome.

Background

The cell exosome is a membrane vesicle released to an extracellular space or biological body fluid after fusion of intracellular multivesicular bodies and cell membranes, participates in a plurality of important physiological or case processes of a human body, has the effects of cell communication, cell migration, promotion of tissue repair or regulation of immune response and the like, has wide application prospect, and the acquisition of high-purity cell exosome is always the premise of developing application research.

The comparison document CN111979089A discloses a large-volume stem cell exosome separation and concentration device and a concentration process, the large-volume stem cell exosome separation and concentration device comprises a separation centrifuge main body, a placing box, a rotating temperature control assembly, a test tube clamping assembly, a test tube, a hinge, a top cover, a sealing lantern ring, a sealing gasket, a magnet block and a bearing, wherein the placing box is arranged on the outer wall of the separation centrifuge main body; according to the device, a plurality of test tubes can be placed on the rotating disc, so that a plurality of groups of centrifugal separation can be performed, and the concentration and extraction of the large-volume stem cell exosomes are facilitated; the efficiency of the exosome concentration and extraction is improved; the sealing ring and the sealing gasket are arranged, so that the sealing in the centrifuge is ensured, and the pollution of cells during separation and concentration is avoided; can use through condenser pipe and thermometer cooperation and can carry out the control of the inside temperature of centrifuge, standardize centrifugation time, centrifugal temperature and centrifugal speed in the technology, improve the concentrated quality of later stage exosome separation.

Among the prior art, stem cell exosome receives the restriction of device when carrying out separation concentration work, leads to inconveniently to dismantle the installation to the cylinder to make inconveniently dismantle abluent problem to the device.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an in order to prior art, stem cell exosome receives the restriction of device when separating concentrated during operation, leads to inconvenient dismantlement installation to the cylinder to make inconvenient dismantlement abluent problem to the device, and provide a separation enrichment facility of stem cell exosome.

The purpose of the utility model can be realized by the following technical scheme:

a separation and concentration device for stem cell exosomes comprises a bottom plate, an outer cylinder and a separation cylinder;

the top surface of the bottom plate is symmetrically provided with support rods, two sides of the outer cylinder are installed on the support rods through fixing blocks, the separating cylinder is positioned in the outer cylinder, the side wall of the separating cylinder is connected with the outer cylinder through a limiting part, and the bottom of the separating cylinder is connected with the rotating shaft through a connecting piece;

the separating cylinder comprises a top ring, a bottom ring, a first reinforcing rib, a first filter screen, a second reinforcing rib, a second filter screen and a central plate; the top ring is positioned right above the bottom ring, a plurality of groups of first reinforcing ribs are arranged in an annular array, the top ends of the first reinforcing ribs are connected with the top ring, the bottom ends of the first reinforcing ribs are connected with the bottom ring, and the first filter screen is arranged among the top ring, the bottom ring and the first reinforcing ribs;

the centre of a circle department of end ring is provided with well core plate, and second strengthening rib annular array is provided with the multiunit, and the one end and the well core plate of second strengthening rib are connected, and the other end and the end ring of second strengthening rib are connected, and the second filter screen setting is between end ring, second strengthening rib and well core plate.

Preferably, the limiting part comprises a rotating ring and a limiting block, the rotating ring is rotatably installed on the inner wall of the outer barrel, the limiting block is arranged at the top of the first reinforcing rib, and a limiting groove matched with the limiting block is formed in the rotating ring.

Preferably, the connecting piece comprises a first connecting block and a second connecting block; the bottom surface at well core plate is installed to the second connecting block, and the bottom of second connecting block is provided with the draw-in groove with first connecting block looks adaptation, and first connecting block is square piece.

Preferably, the top surface of the bottom plate is provided with a driving motor, the output end of the driving motor is connected with a rotating shaft, the rotating shaft penetrates through the bottom surface of the outer barrel and is rotatably connected with the outer barrel, and the first connecting block is installed at the top end of the rotating shaft.

Preferably, the bottom surface of the outer cylinder is provided with a liquid outlet, and a liquid outlet pipe is installed at the liquid outlet.

Compared with the prior art, the beneficial effects of the utility model are that: adding the crude stem cell exosome liquid into a separation cylinder, starting a driving motor to work to drive a rotating shaft to rotate, driving the separation cylinder to rotate through a connecting piece, rotating the separation cylinder along the inner wall of an outer cylinder through a limiting piece, separating the crude stem cell exosome liquid under the action of the rotating separation cylinder, and discharging the concentrated stem cell exosome liquid into the outer cylinder along a liquid discharge pipe; after separation concentration work is accomplished, can directly take out the cylinder from the urceolus and wash, have the convenience and dismantle abluent advantage to the cylinder, then, align the spacing groove of stopper and swivel, then place the cylinder in, simultaneously, first connecting block is located the draw-in groove of second connecting block, alright in order to separate concentration work once more, so the utility model discloses a separation concentration device has the advantage of conveniently installing dismantlement and cleaning work.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural view of the inside of the outer barrel of the present invention.

Fig. 3 is a bottom view of the separating cylinder of the present invention.

In the figure: 1. a base plate; 2. a support bar; 3. a fixed block; 4. a drive motor; 5. an outer cylinder; 6. a liquid discharge pipe; 7. a rotating shaft; 8. a first connection block; 9. a separation cylinder; 10. a top ring; 11. a bottom ring; 12. a first reinforcing rib; 13. a first filter screen; 14. a second reinforcing rib; 15. a second filter screen; 16. a center plate; 17. a card slot; 18. a second connecting block; 19. rotating the ring; 20. and a limiting block.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

Referring to fig. 1-3, a device for separating and concentrating stem cell exosomes includes a bottom plate 1, an outer cylinder 5, a separating cylinder 9;

the top surface of the bottom plate 1 is symmetrically provided with support rods 2, two sides of an outer cylinder 5 are installed on the support rods 2 through fixing blocks 3, a separating cylinder 9 is positioned in the outer cylinder 5, the side wall of the separating cylinder 9 is connected with the outer cylinder 5 through a limiting piece, and the bottom of the separating cylinder 9 is connected with a rotating shaft 7 through a connecting piece;

the separating cylinder 9 comprises a top ring 10, a bottom ring 11, a first reinforcing rib 12, a first filter screen 13, a second reinforcing rib 14, a second filter screen 15 and a central plate 16; the top ring 10 is positioned right above the bottom ring 11, a plurality of groups of first reinforcing ribs 12 are arranged in an annular array, the top ends of the first reinforcing ribs 12 are connected with the top ring 10, the bottom ends of the first reinforcing ribs 12 are connected with the bottom ring 11, and the first filter screen 13 is arranged among the top ring 10, the bottom ring 11 and the first reinforcing ribs 12;

the center of the bottom ring 11 is provided with a center plate 16, a plurality of groups of second reinforcing ribs 14 are arranged in an annular array, one end of each second reinforcing rib 14 is connected with the center plate 16, the other end of each second reinforcing rib 14 is connected with the bottom ring 11, and a second filter screen 15 is arranged among the bottom ring 11, the second reinforcing ribs 14 and the center plate 16.

The locating part includes swivel 19, stopper 20, and swivel 19 rotates and installs on the inner wall of urceolus 5, and the top of first strengthening rib 12 is provided with stopper 20, is provided with the spacing groove with stopper 20 looks adaptation on the swivel 19.

The connecting piece comprises a first connecting block 8 and a second connecting block 18; the second connecting block 18 is installed on the bottom surface of the central plate 16, a clamping groove 17 matched with the first connecting block 8 is arranged at the bottom of the second connecting block 18, and the first connecting block 8 is a square block.

The top surface of bottom plate 1 is provided with driving motor 4, and driving motor 4's output is connected with pivot 7, and pivot 7 runs through the bottom surface of urceolus 5 to rotate with urceolus 5 and be connected, first connecting block 8 is installed on the top of pivot 7.

The bottom surface of the outer cylinder 5 is provided with a liquid outlet, and a liquid outlet pipe 6 is arranged at the liquid outlet.

The utility model discloses a theory of operation: adding the crude stem cell exosome liquid into a separation cylinder 9, starting a driving motor 4 to work, driving a rotating shaft 7 to rotate, driving the separation cylinder 9 to rotate through a connecting piece by the rotating shaft 7, and rotating the separation cylinder 9 along the inner wall of an outer cylinder 5 through a limiting piece by the separation cylinder 9, so that the crude stem cell exosome liquid is separated under the action of the rotating separation cylinder 9, and the concentrated stem cell exosome liquid flows into the outer cylinder 5 and is discharged along a liquid discharge pipe 6; after the concentrated work of separation is accomplished, can directly take out the knockout drum 9 from urceolus 5 and wash, have the convenience and dismantle abluent advantage to knockout drum 9, then, align stopper 20 and swivel 19's spacing groove, then place the knockout drum 9 in urceolus 5, simultaneously, first connecting block 8 is located the draw-in groove 17 of second connecting block 18, alright with the concentrated work of separating once more, so the utility model discloses a separation enrichment facility has the advantage of conveniently installing dismantlement and cleaning work.

The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not exhaustive and do not limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A separation enrichment facility of stem cell exosome which characterized in that: comprises a bottom plate (1), an outer cylinder (5) and a separating cylinder (9);

the top surface of the bottom plate (1) is symmetrically provided with support rods (2), two sides of the outer cylinder (5) are mounted on the support rods (2) through fixing blocks (3), the separating cylinder (9) is positioned in the outer cylinder (5), the side wall of the separating cylinder (9) is connected with the outer cylinder (5) through a limiting piece, and the bottom of the separating cylinder (9) is connected with the rotating shaft (7) through a connecting piece;

the separating cylinder (9) comprises a top ring (10), a bottom ring (11), a first reinforcing rib (12), a first filter screen (13), a second reinforcing rib (14), a second filter screen (15) and a central plate (16); the top ring (10) is positioned right above the bottom ring (11), a plurality of groups of first reinforcing ribs (12) are arranged in an annular array mode, the top ends of the first reinforcing ribs (12) are connected with the top ring (10), the bottom ends of the first reinforcing ribs (12) are connected with the bottom ring (11), and the first filter screen (13) is arranged among the top ring (10), the bottom ring (11) and the first reinforcing ribs (12);

the center of the circle of the bottom ring (11) is provided with a center plate (16), a plurality of groups of second reinforcing ribs (14) are arranged in an annular array mode, one ends of the second reinforcing ribs (14) are connected with the center plate (16), the other ends of the second reinforcing ribs (14) are connected with the bottom ring (11), and a second filter screen (15) is arranged among the bottom ring (11), the second reinforcing ribs (14) and the center plate (16).

2. The device for separating and concentrating the exosomes of the stem cells according to claim 1, wherein the limiting member comprises a rotating ring (19) and a limiting block (20), the rotating ring (19) is rotatably mounted on the inner wall of the outer cylinder (5), the limiting block (20) is arranged at the top of the first reinforcing rib (12), and a limiting groove matched with the limiting block (20) is arranged on the rotating ring (19).

3. The device for separating and concentrating the exosomes of the stem cells according to claim 1, wherein the connecting piece comprises a first connecting block (8), a second connecting block (18); the second connecting block (18) is arranged on the bottom surface of the central plate (16), a clamping groove (17) matched with the first connecting block (8) is formed in the bottom of the second connecting block (18), and the first connecting block (8) is a square block.

4. The device for separating and concentrating the exosomes of the stem cells according to claim 1, characterized in that a driving motor (4) is arranged on the top surface of the bottom plate (1), the output end of the driving motor (4) is connected with a rotating shaft (7), the rotating shaft (7) penetrates through the bottom surface of the outer barrel (5) and is rotatably connected with the outer barrel (5), and the first connecting block (8) is installed on the top end of the rotating shaft (7).

5. The device for separating and concentrating the stem cell exosomes according to claim 1, wherein a liquid outlet is arranged on the bottom surface of the outer cylinder (5), and a liquid outlet pipe (6) is arranged at the liquid outlet.