CN214965713U - Smell stimulation and electrophysiological recording device for small animal coupled respiration - Google Patents
Smell stimulation and electrophysiological recording device for small animal coupled respiration Download PDFInfo
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- CN214965713U CN214965713U CN202022821236.5U CN202022821236U CN214965713U CN 214965713 U CN214965713 U CN 214965713U CN 202022821236 U CN202022821236 U CN 202022821236U CN 214965713 U CN214965713 U CN 214965713U
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Abstract
The utility model discloses a smell stimulation and electrophysiological recording device for small animal coupled respiration, which comprises a nasal ventilation tube, a multi-channel smell generating device, a respiration recording device, an electrophysiological recording device, an activity monitoring device and a micro-control computer; the electrophysiological recording device records the electrophysiological signals of the animal and transmits the signals to the micro-control computer. The utility model can automatically control, record and analyze data in the experimental process by using the control panel in the system, so that the ordinary animals in free activity state or animals in other diseases related modes can receive odor stimulation in different olfaction related behaviouristic experiments, and electrophysiological signals, respiratory signals and motion trail signals can be synchronously monitored in the process; therefore, a new thought is provided for researching the olfactory function and the internal neural mechanism of the animal, and the method is also a powerful tool for verifying the effectiveness of the treatment and research methods of olfactory and related brain diseases.
Description
Technical Field
The utility model belongs to the technical field of the animal experiment, in particular to smell stimulation and electrophysiological recording device that toy coupling was breathed.
Background
The sense of smell plays an important role in searching food, marking territory, searching spouse, avoiding natural enemies and the like, so the sense of smell has important significance for animals. In the biomedical field, in the research of olfaction of animals (such as model animals like rats and mice) and various behaviours, the traditional experimental mode mostly adopts a passive air supply mode with a fixed head state, so that the artificial restraint mode may affect the brain state of the animals and is not beneficial to reflecting the real behaviors and results of the animals. At present, only a few of the existing air supply modes which are freely movable and fixed at the box body position are adopted, so that the defects of poor anti-interference capability, long period, poor repeatability, complex operation and the like of synchronous recording of data such as behavior and in-vivo electrophysiology and the like are caused, and complex odor and behavior research cannot be carried out. Sampling of simultaneous breaths is crucial for odor perception, but existing studies largely overlook this problem. The smell perception efficiency can be greatly improved by supplying gas when the animal inhales but not when exhales, but the coupling of gas supply and respiration is still the technical difficulty of olfactory research at home and abroad, particularly on small animals in free activity state.
SUMMERY OF THE UTILITY MODEL
To the above, an object of the utility model is to provide a stable, with breathe accurate coupling, can show in real time and monitor each item recording data's smell stimulation and synchronous electrophysiological recording device that free activity state toy couples and breathes for gas.
In order to realize the utility model discloses a technical scheme that the purpose adopted is: a smell stimulation and electrophysiological recording device for small animal coupled respiration comprises a nasal ventilation tube fixed on the nose of a small animal and used for receiving smell, a multi-channel smell generating device, a respiration recording device, an electrophysiological recording device, an activity monitoring device and a micro-control computer; the multi-channel smell generating device is a smell delivery device capable of giving stable air flow, and the nose ventilation tube at the head of the small animal receives different delivered smells through a hose; the breath recording device detects the breath state of the animal and transmits a breath signal to the control panel, and the control panel judges the expiration phase and the inspiration phase and sends an instruction to control the air valve switch to accurately give out smell when the animal inhales; the electrophysiological recording device records electrophysiological signals of the animal and transmits the signals to the micro-control computer; the micro-control computer is a terminal for collecting, processing and displaying data of odor signals, electrophysiological signals, respiratory signals and motion track signals, and is also provided with related control and monitoring software.
Further, the nasal ventilation tube comprises a stainless steel air tube, a connecting base and a thin hose; the stainless steel trachea is divided into left and right two, and two nostrils about the toy are pressed close to respectively to one end, and the other end passes through the thin hose to be connected with smell generation device output, and two stainless steel tracheas keep parallel and are fixed in the toy nose through connecting the base.
Further, the multi-channel scent generating apparatus includes: the inlet end of the carrier gas input channel is sequentially connected with an air pump and a flowmeter, and the outlet end of the carrier gas input channel is connected with a multi-branch pipe; the inlet end of the stable airflow channel is connected with a multi-branch pipe connected with an air pump, a flowmeter, an electromagnetic valve and an odor output multi-branch pipe, the stable airflow channel is connected with the odor channel in parallel, the air flow is kept to be the same as that of the odor channel, the stable airflow channel is connected with the odor output channel through two-way electromagnetic valves, and the outlet of the two-way electromagnetic valve is directly pointed to a target animal; the inlet end of the smell output channel is the smell output multi-branch pipe, and the outlet end of the smell output channel is directly connected with the nasal vent pipe; the odor channels respectively consist of a flowmeter, two-way electromagnetic valves and an odor bottle arranged between the two-way electromagnetic valves which are sequentially connected, the inlet ends of the odor channels are connected with a multi-branch pipe connected with an air pump in parallel, and the outlet ends of the odor channels are connected with the odor output multi-branch pipe in parallel.
Furthermore, the breath recording device comprises a breathing sleeve, a pressure sensor, an amplifier and a thin hose; the breathing sleeve consists of a connecting sleeve and an inserting core, the inserting core needle is inserted into the sleeve, one end of a conduit of the inserting core needle is inserted into a nasal cavity of a mouse at a proper position, and the other end of the conduit is connected with a hose with good air tightness and proper diameter.
Furthermore, the pressure sensor is a single-tube structure and comprises an inlet end and an outlet end; the inlet end directly receives the physical signal of air pressure change generated by the nasal cavity respiration of the small animal, and the outlet end outputs the electrical signal into the amplifier.
Furthermore, the amplifier is a device for amplifying the recorded breathing signal, the input end of the amplifier is connected with the pressure sensor, the output end of the amplifier is connected with the control board, so that the amplified breathing signal is transmitted to the control board, and the control board judges the breathing/inhaling phase of the breathing signal and then waits for sending the breathing instruction.
Furthermore, the control panel also comprises a feedback control circuit, wherein the analog signal input end of the feedback control circuit receives the breathing signal and transmits the breathing signal, the output end of the feedback control circuit sends a breathing instruction to enter the field effect tube after the judgment of the breathing and inspiration time is made in the operation program, and the two-way electromagnetic valves of the smell channel in the multi-channel smell generating device respectively output and change the control switch through the voltage of the field effect tube.
Furthermore, the electrophysiological recording device comprises a multi-channel microelectrode, a front-end amplifier, a connecting line and an electrophysiological signal amplifier, wherein the microelectrode can monitor electrophysiological signals in a brain area, and the microelectrode transmits electrophysiological signals with high signal-to-noise ratio to the signal amplifier through the front-end amplifier and the connecting line; the signal is amplified and transmitted to a data acquisition card, the electrophysiological signal is displayed on a display, and the data is stored in a micro-control computer after being processed by analysis software.
Further, the activity monitoring device comprises a camera for monitoring and recording the activity of the small animal; the camera is connected with the micro-control computer, transmits the collected behavior activity signals to the corresponding display for displaying and stores the data in the micro-control computer.
Furthermore, the data acquisition module is used for recording and storing the whole process of the data acquisition module in the micro-control computer in an experiment, automatically acquiring the breathing, electric signals and motion track data of the small animal when the experiment is started, and transmitting the signals to the micro-control computer for displaying and processing.
The utility model discloses owing to adopt above technical scheme, following beneficial effect has:
1. the utility model discloses a software and hardware technique up-to-date and mainstream, maneuverability is strong, and error range is little, and the functioning speed is fast, and the interactive interface is friendly.
2. The nose ventilation pipe is simple in manufacturing steps, small in wound and portable in installation for animals, and the free moving state of the animals is restored to the maximum extent.
3. The odor generating device is additionally provided with a stable airflow channel, the stability of the output gas flow is ensured, the mechanical sensitivity stimulation to animals is reduced, and each channel is relatively independent and is convenient to install, disassemble and replace.
4. Through the high power of operation of control panel, can accurately snatch the time item of breathing in of animal respiratory process and trigger control and give gas, guarantee to breathe in and trigger and give gas the alignment of event in time.
5. The information of the type, duration, interval time and the like of the smell can be set arbitrarily through an editing function, and random gas supply can be realized through a method of inputting random numbers externally.
6. The odor channel can be expanded and modified by expanding the control panel and the feedback circuit, so that the odor channel can adapt to various specific experimental requirements, particularly complex olfactory related odor behavior experiments.
7. The recording device for the respiration records data accurately and reliably, the embedding of the respiration casing pipe has small damage to the small animal and small occupied volume, and the minimally invasive and light weight of the experiment operation is realized.
8. The body electrophysiological data, the breathing signal, the gas supply signal and the motion trail signal are simultaneously acquired and recorded, so that the study on the subtle response under the accurate time information and the difference change on a long time axis is facilitated, and a new possibility is provided for studying the behavioral neural mechanism and related diseases and drug test olfaction tests.
9. The high frame rate camera of the activity monitoring device can monitor the motion trail of the animal in the experimental process at any time and can capture characteristic information under a specific behavior paradigm, so that various olfaction-related behavioral tests can be conveniently researched.
10. The data acquisition module supports multichannel data record, and the expansibility is stronger, and enlargeability, filtering capability can be adjusted by oneself, can self-defined collection channel, and the operation is simpler provides very big convenience to the show of experimental result.
Drawings
Fig. 1 is a schematic structural diagram of the novel odor stimulation and synchronous electrophysiological recording device for the coupled respiration of the free-moving small animal of the present invention.
Fig. 2 is a schematic view of an odor generating device.
FIG. 3 is a schematic diagram of a control board feedback and control circuit.
Fig. 4 is a schematic diagram of signal acquisition and processing.
In the figure, 1-a nose ventilation pipe, 2-a connecting base, 3-a breathing sleeve, 4-a multi-channel microelectrode, 5-a camera, 6-a micro-control computer, 7-an air pump, 8-an air volume regulator, 9-a flow meter, 10-an electromagnetic valve, 11-a smell bottle, 12-a multi-branch pipe, 13-a control plate, 14-a solderless bread board, 15-a field effect pipe and 16-an air path control electromagnetic valve.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1, an odor stimulation and electrophysiological recording device for coupled respiration of a small animal comprises a nasal ventilation tube fixed on the nose of the small animal for receiving odor, a multi-channel air device, a respiration recording device, an electrophysiological recording device, an activity monitoring device and a micro-control computer; the multi-channel smell generating device is a smell delivery device capable of providing stable air flow, and the left and right nasal ventilation tubes 1 at the head of the small animal are fixed through the connecting base 2 and receive different delivered smells from the hose; the breath recording device is inserted into the nasal cavity of a mouse through a breathing sleeve 3, the other end of the breath recording device is connected with a hose with good air tightness and proper diameter, the breath recording device is connected to a pressure sensor and monitors the change of a breath signal, and then the signal is amplified through an amplifier, at the moment, on one hand, the control panel judges the expiration phase and the inspiration phase and sends an instruction to control the air valve switch, on the other hand, a data acquisition card can acquire the respiratory frequency and the amplitude signal of the breath and transmit the respiratory frequency and the amplitude signal to a micro-control computer, the breath signal is displayed on a display in real time, and meanwhile, the data is stored. The electrophysiological recording device is to implant the microelectrode 4 into the target brain area of the animal, monitor the electrophysiological signals of the unit discharge and the field potential of the neurons in the brain area, and transmit the signals to the micro-control computer through the front-end amplifier and the signal amplifier; the micro-control computer is a terminal for collecting, processing and displaying data of odor signals, electrophysiological signals, respiratory signals and motion track signals, and is also provided with related control and monitoring software.
As shown in fig. 2, the odor generating apparatus includes:
the inlet end of the carrier gas input channel is sequentially connected with an air pump 7, a regulator 8 capable of regulating the air quantity of the air pump and a flowmeter 9, and the outlet end of the carrier gas input channel is connected with a multi-branch pipe 12;
a stable airflow channel ch0, the inlet end of which is connected with a multi-branch pipe connected by an air pump, a flowmeter, an electromagnetic valve 10, an air odor bottle 11 and an odor output multi-branch pipe 12, the stable airflow channel is connected with the odor channel in parallel, the same air quantity as the odor channel is kept, and is connected with the odor output channel through two-way electromagnetic valves, the outlet of the two-way electromagnetic valve is connected with the multi-branch pipe and directly points to the target animal;
the inlet end of the smell output channel is the smell output multi-branch pipe, and the outlet end of the smell output channel is directly connected with the nasal vent pipe;
the odor control device comprises a plurality of odor channels (ch1-chn), wherein each odor channel is respectively composed of a flowmeter, two-way electromagnetic valves and an odor bottle arranged between the two-way electromagnetic valves which are sequentially connected, the inlet end of each odor channel is connected with a manifold connected with an air pump in parallel, and the outlet end of each odor channel is connected with the odor output manifold in parallel.
Before delivering the smell, the air pump 7 and each electromagnetic valve 10 are opened, and the gas quantity is ensured to be stabilized on the scale of the required value through the gas quantity regulator 8 and each flow meter 11. The gas circuit flow is as follows;
1. the carrier gas output channel carries and blows out the gas in the odor output manifold to the target animal with a fixed gas amount, and the channel is kept open continuously.
2. In the stable air flow channel, the air path is also in an open state at the beginning of the experiment and is opened when the smell channel is closed, so that the non-delay switching between the air path and the smell channel is realized, the air flow from the smell output multi-branch pipeline to the nasal cavity of the animal is ensured to be always constant, and the mechanical stimulation to the animal is reduced.
3. In the smell passageway, after the experiment began, when detecting the first phase of breathing in of animal, the control panel triggers and opens smell passageway solenoid valve, and certain flow's air is come out the gas in the smell bottle and is gone into output multi-branch way pipe and reach the animal nasal cavity through the solenoid valve.
In the embodiment, the air quantity is balanced by adjusting the flowmeter and the air quantity regulator, the odor concentration of the gas sprayed out after each channel passes through the odor bottle is kept stable, and the odor concentration of each odor channel before the test is calibrated by the photoionization gas detector.
The utility model discloses used two-way solenoid valve is the silence version, does not produce the sound stimulation to the animal, avoids producing the influence to the behavioural result.
The utility model discloses the used trachea of gas circuit adopts the PTEE material, in time changes after using, avoids the smell to pollute the gas circuit.
The utility model discloses a method of hardware program external input random number accomplishes that each smell passageway opens the order random, avoids fixed smell sequence memory to cause the deviation to the experimental result.
As shown in fig. 3, before the experiment starts, the control board feedback and control circuit transmits key parameters such as air supply time, interval time, random sequence of opening the odor channel and the like to the control board hardware 13 through the serial port according to the requirements of the relevant experimental paradigm, and determines the customized standardized odor information mark. In the experiment, the control panel continuously receives the respiration signals transmitted by the respiration recording device, the inspiration phase in a unit time window is accurately captured by taking the zero crossing point of descending branch as a judgment standard, each field effect tube 14 is directly connected with the output port of the control panel, the field effect tubes and the wiring in hardware are integrated on a solderless bread board 15, and the control circuit controls the opening and closing of the two-way electromagnetic valve 16 of each odor channel through the change of output voltage.
As shown in fig. 1 and 4, the motion trace signals are collected by a camera, and the camera is arranged at a fixed position in the animal activity space and continuously recorded in the experimental time window. Before the experiment begins, the shooting visual field and the focal length can be finely adjusted, and the definition of a recorded picture and the stability of the visual distance are ensured. The camera passes through the data acquisition module with the signal and passes to the micro-control computer, and the motion of accessible display detection animal.
The utility model relates to a smell stimulation and electrophysiological recording device for small animal coupled respiration, which comprises a nasal ventilation tube fixed on the head of a small animal and used for receiving smell, a multi-channel smell generating device, a respiration recording device, an electrophysiological recording device, an activity monitoring device, a control panel and a micro-control computer; the smell generating device is a smell delivery device capable of giving stable air flow, and the nose ventilation tube at the head of the small animal receives different delivered smells through a hose; the breath recording device detects the breath state of the animal and transmits a breath signal to the control panel, and the control panel judges the expiration phase and the inspiration phase and sends an instruction to control the opening and closing of the air valve, so that the smell is accurately given when the animal inhales; the electrophysiological recording device records electrophysiological signals of the animal and transmits the signals to the micro-control computer; the micro-control computer is a terminal for data acquisition, processing and display. The utility model can automatically control, record and analyze data in the experimental process by using the control panel in the system, so that the ordinary animals in free activity state or animals in other diseases related modes can receive odor stimulation in different olfaction related behaviouristic experiments, and electrophysiological signals, respiratory signals and motion trail signals can be synchronously monitored in the process; therefore, a new thought is provided for researching the olfactory function and the internal neural mechanism of the animal, and the method is also a powerful tool for verifying the effectiveness of the treatment and research methods of olfactory and related brain diseases.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a smell stimulation and electrophysiological recording device of toy coupling breathing which characterized in that: comprises a nasal ventilation tube fixed on the head of a small animal and used for receiving smell, a multi-channel smell generating device, a breath recording device, an electrophysiological recording device, an activity monitoring device, a control panel and a micro-control computer; the multi-channel scent generating apparatus is a scent delivery device capable of giving a stable flow of gas; the breath recording device is connected with the control panel; the control panel is connected with the smell generating device, the smell generating device is connected with the nasal ventilation tube through a hose, and the electrophysiological recording device is connected with the micro-control computer.
2. The scent stimulation and electrophysiological recording device for coupled breathing in small animals of claim 1, wherein: the nasal ventilation pipe comprises a stainless steel air pipe, a connecting base and a thin hose; the stainless steel trachea is divided into a left trachea and a right trachea, one end of the stainless steel trachea is respectively close to the left nostril and the right nostril of the small animal, the other end of the stainless steel trachea is connected with the output end of the air supply device through a thin hose, and the two stainless steel tracheas are kept parallel through the connecting base and are fixed on the head of the small animal.
3. The scent stimulation and electrophysiological recording device for coupled breathing in small animals of claim 1, wherein: the multi-channel scent generating apparatus includes:
the inlet end of the carrier gas input channel is sequentially connected with an air pump and a flowmeter, and the outlet end of the carrier gas input channel is connected with a multi-branch pipe;
the inlet end of the smell output channel is the multi-branch pipe, and the outlet end of the smell output channel is directly connected with the nasal ventilation pipe;
the inlet end of the stable airflow channel is connected with a multi-branch pipe connected with an air pump, a flowmeter, an electromagnetic valve and an odor output multi-branch pipe, the stable airflow channel is connected with the odor channel in parallel, the air flow is kept to be the same as that of the odor channel, the stable airflow channel is connected with the odor output channel through two-way electromagnetic valves, and the outlet of the two-way electromagnetic valve is directly pointed to a target animal;
the odor channels respectively consist of a flowmeter, two-way electromagnetic valves and an odor bottle arranged between the two-way electromagnetic valves which are sequentially connected, the inlet ends of the odor channels are connected with a multi-branch pipe connected with an air pump in parallel, and the outlet ends of the odor channels are connected with the odor output multi-branch pipe in parallel.
4. The scent stimulation and electrophysiological recording device for coupled breathing in small animals of claim 1, wherein: the respiration recording device comprises a respiration casing pipe, a pressure sensor, an amplifier and a thin hose; the breathing sleeve consists of a connecting sleeve and a plug core, the plug core needle is inserted into the sleeve, the hose is sequentially connected with the pressure sensor and the amplifier, and the amplifier is connected with the control panel.
5. The device for odor stimulation and electrophysiological recording of breath coupled to small animals of claim 4, wherein the device further comprises: the pressure sensor is of a single-tube structure and comprises an inlet end and an outlet end; the inlet end directly receives the physical signal of air pressure change generated by the nasal cavity respiration of the small animal, and the outlet end outputs the electrical signal into the amplifier.
6. The scent stimulation and electrophysiological recording device for coupled breathing in small animals of claim 1, wherein: the electrophysiological recording device comprises a multi-channel microelectrode, a front-end amplifier, a connecting line and an electrophysiological signal amplifier, wherein the microelectrode can monitor electrophysiological signals of a brain area, and the microelectrode transmits electrophysiological signals with high signal-to-noise ratio to the signal amplifier through the front-end amplifier and the connecting line; the signal amplifier is connected with the data acquisition card.
7. The scent stimulation and electrophysiological recording device for coupled breathing in small animals of claim 1, wherein: the movement monitoring device is used for detecting the movement track of the small animal in the recording process and comprises a camera for monitoring and recording the movement of the small animal; the camera is connected with the micro-control computer, transmits the collected behavior activity signals to the corresponding display for displaying and stores the data in the micro-control computer.
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