CN214961710U - Wheat crop anther in-vitro preservation and culture box - Google Patents

Wheat crop anther in-vitro preservation and culture box Download PDF

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Publication number
CN214961710U
CN214961710U CN202121052766.6U CN202121052766U CN214961710U CN 214961710 U CN214961710 U CN 214961710U CN 202121052766 U CN202121052766 U CN 202121052766U CN 214961710 U CN214961710 U CN 214961710U
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room
cultivate
preservation
culture
tube rack
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CN202121052766.6U
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李葆春
许静玉
王化俊
姚立蓉
汪军成
孟亚雄
马小乐
司二静
孙祥岭
张宝蓉
金丹青
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Gansu Agricultural University
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Gansu Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The utility model relates to a wheat crop anther in vitro is preserved and incubator, its structure includes: cultivate room and save the room, cultivate the room and keep the room and separate by the heat insulating board, it is equipped with the observation window to cultivate the room top, the chamber door of cultivateing room and save the room can be opened through handle and loose-leaf, it is equipped with control panel respectively to cultivate the room and keep the room, the box side is equipped with lithium cell and solar cell panel, there is the ultraviolet lamp in the cultivation room, the day is closed the lamp, it is equipped with semiconductor refrigerating plant respectively to cultivate the room and keep the room, there is the test-tube rack support in, be used for fixed test-tube rack, the test-tube rack can be dismantled, change. The method is simple to operate, and can meet the conditions of isolated pollen preservation and culture under different conditions.

Description

Wheat crop anther in-vitro preservation and culture box
Technical Field
The utility model relates to a crops cross breeding instrument, concretely relates to wheat crop separation anther is preserved and incubator.
Background
In order to effectively solve the problem of low yield and quality in wheat crop production, realize production modernization of wheat crops, and widely apply a cross breeding technology to production practice, the cross breeding technology is beneficial to improving the quality of the wheat crops and improving the capability of resisting adversity stress such as disease, insect and weed damage, and the like, thereby reducing the damage rate of the wheat crops, reducing the pollution and damage to the natural environment, finally realizing the purposes of increasing the yield of grains per unit area and providing guarantee for national grain safety. In China, the cross breeding technology has a long history and occupies an important position in the agricultural field, wherein the new wheat crop variety cultivated by the cross breeding technology brings significant practical benefits to farmers.
The prior art has the problems that: in the crossbreeding of wheat crops, the artificial ear trimming and emasculation are usually adopted before flowering, and then the ear twisting method, the pollen dipping method and the like are adopted to obtain hybrid seeds, but the problems of inconsistent flowering period, pollen pollution, low cross pollination efficiency and the like are faced in the artificial pollination process. Therefore, the culture and preservation device for the isolated anther of the wheat crop, which is simple to operate and can preserve the isolated pollen and carry out the culture at any time, has important practical significance.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model discloses a following technical scheme:
a wheat crop isolated pollen culture and preservation box structurally comprises: cultivate room and save the room, cultivate the room and keep the room and separate by the heat insulating board, it is equipped with the observation window to cultivate the room top, it is equipped with the chamber door and can open through handle and loose-leaf to cultivate room and save the room top, it is equipped with respectively to cultivate room control panel and save room control panel with the save room to cultivate room, lithium cell and solar cell panel provide the electric energy for ultraviolet lamp, fluorescent lamp and semiconductor refrigerating plant, it is equipped with the test-tube rack support in room and the save room to cultivate the room, the test-tube rack can be fixed to the test-tube rack support.
Preferably, the box bodies and the box doors of the culture chamber and the preservation chamber are made of high-quality cold-rolled steel plates, and are subjected to rust-proof and corrosion-proof treatment, ultraviolet rays cannot penetrate through the box bodies, and the observation windows of the culture chamber are made of double-layer hollow glass to ensure the heat-insulating property of the culture chamber.
Preferably, seal strips are arranged at the door parts of the culture chamber and the preservation chamber, and are made of polyvinyl chloride (PVC), so that the culture chamber and the preservation chamber are corrosion-resistant, firm and durable, and have high toughness.
Preferably, the culture chamber adopts a mirror surface stainless steel inner container, and four corners are in semi-arc transition, so that the culture chamber is convenient to clean and disinfect; the heat-insulating layer of the preservation room adopts a vacuum heat-insulating plate (VIP plate), and has the advantages of low heat conductivity coefficient, long heat-insulating period, thin thickness, small volume, light weight and energy conservation.
Preferably, the temperature control system of the culture chamber and the preservation chamber mainly comprises a semiconductor refrigeration piece, also called a thermoelectric refrigeration piece, is a heat pump, is connected with a temperature controller and is provided with a heat dissipation device. TES1-4902 from Guangzhou electro-technology Limited is preferably used.
Preferably, the culture chamber and the preservation chamber are respectively provided with an independent intelligent temperature control system, and when the temperature is 1 ℃ higher than the set temperature, the main heating system of the device is automatically cut off through an electromagnetic relay.
Preferably, but lithium cell rechargeable uses, and when outdoor condition electric quantity was not enough, solar cell panel can expand suitable angle through the loose-leaf, carries out solar charging, avoids the electric quantity not enough to cause the damage to cultivateing or save the material, and collapsible accomodating is not occupation space in the box side when not needing.
Preferably, supporting detachable test-tube rack adopts stainless steel, can place on the test-tube rack support during the use, and the test-tube rack square can be dismantled and the adjustment size, satisfies the test tube of different bore sizes and uses.
Preferably, the lithium battery and the solar panel supply power for the ultraviolet lamp, the fluorescent lamp and the semiconductor refrigerating device.
Preferably, the ultraviolet lamp and the fluorescent lamp are USB power supply bulbs.
Preferably, the control panel of the culture room and the control panel of the preservation room are provided with switches for controlling the ultraviolet lamp, the fluorescent lamp and the semiconductor refrigerating device and a temperature control electromagnetic relay.
Has the advantages that: the invention has simple operation, can culture and preserve the isolated pollen at any time, avoids the hybridization failure caused by different florescence during the hybridization work, has small volume of the semiconductor refrigerating device, and can refrigerate and heat.
Drawings
FIG. 1 is a schematic diagram of the external structure of an isolated pollen cultivation and preservation box for wheat crops;
FIG. 2 is a schematic diagram of the internal structure of an isolated pollen cultivation and preservation box for wheat crops;
FIG. 3 is a schematic structural view of a detachable test tube rack matched with a wheat crop isolated pollen culture and preservation box;
the device comprises a culture room 1, a storage room 2, an observation window 3, a heat insulation board 4, a handle 5, a culture room control panel 6, a storage room control panel 7, a lithium battery 8, a solar cell panel 9, a hinge 10, a box door 11, an ultraviolet lamp 12, a fluorescent lamp 13, a semiconductor refrigerating device 14, a test tube rack support 15 and a test tube rack 16.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the following detailed description of the embodiments of the present invention will be described with reference to the accompanying drawings. Examples of these preferred embodiments are illustrated in the accompanying drawings. The embodiments of the invention shown in the drawings and described in accordance with the same are merely exemplary and the invention is not limited to these embodiments. Here, it should be further noted that, in order to avoid obscuring the technical solution of the present invention by unnecessary details, only the structures and/or processing steps closely related to the solution according to the present invention are shown in the drawings, and other details that are not relevant are omitted.
Example 1
This embodiment provides a wheat crop isolated pollen culture and preservation case, as shown in fig. 1-3, its structure includes:
cultivate room 1 and save room 2, cultivate room 1 and save room 2 and be separated by heat insulating board 4, cultivate 1 top in room and be equipped with observation window 3, cultivate 1 and save 2 tops in room and be equipped with chamber door 11 and can open through handle 5 and loose-leaf 10, cultivate 1 and save room 2 and be equipped with respectively and cultivate 6 room control panel and save 7 room control panel, lithium cell 8 and solar cell panel 9 give ultraviolet lamp 12, fluorescent lamp 13 and semiconductor refrigerating plant 14 provide the electric energy, be equipped with test-tube rack support 15 in cultivate 1 and the save room 2, test-tube rack support 15 can fix test-tube rack 16.
Furthermore, the box bodies and the box doors of the culture room 1 and the preservation room 2 are made of high-quality cold-rolled steel plates, and are subjected to rust-proof and corrosion-proof treatment, ultraviolet rays cannot penetrate through the box bodies, and the observation window 3 of the culture room 1 is made of double-layer hollow glass to ensure the heat preservation performance of the culture room.
Furthermore, seals are arranged at the box doors 11 of the culture room 1 and the preservation room 2, and are made of polyvinyl chloride (PVC), so that the box is corrosion-resistant, firm and durable, and has strong toughness.
Furthermore, the culture chamber 1 adopts a mirror surface stainless steel inner container, and four corners are in semi-arc transition, so that the cleaning and the disinfection are convenient; the heat-insulating layer of the preservation room adopts a vacuum heat-insulating plate (VIP plate), and has the advantages of low heat conductivity coefficient, long heat-insulating period, thin thickness, small volume, light weight and energy conservation.
Furthermore, the temperature control system of the culture chamber 1 and the preservation chamber 2 mainly comprises a semiconductor refrigeration piece, also called a thermoelectric refrigeration piece, is a heat pump, is connected with a temperature controller and is provided with a heat dissipation device. TES1-4902 from Guangzhou electro-technology Limited is preferably used.
Furthermore, the culture room 1 and the preservation room 2 are respectively provided with an independent intelligent temperature control system, and when the temperature is higher than the set temperature by 1 ℃, a main heating system of the device is automatically cut off through an electromagnetic relay.
Further, the rechargeable use of lithium cell 8, when outdoor condition electric quantity is not enough, solar cell panel 9 can expand suitable angle through loose-leaf 10, carries out solar charging, avoids the electric quantity not enough to cause the damage to cultivateing or save material, and collapsible accomodating is in the box side when not needing, occupation space not.
Further, supporting detachable test-tube rack 16 adopts stainless steel, can place on the test-tube rack support during the use, and the test-tube rack square can be dismantled and the adjustment size, satisfies the test tube of different bore sizes and uses.
Further, the lithium battery 8 and the solar cell panel 9 supply power to the ultraviolet lamp 12, the fluorescent lamp 13 and the semiconductor refrigeration device 14.
Further, the ultraviolet lamp 12 and the fluorescent lamp 13 are USB-powered bulbs.
Furthermore, the control panel 6 of the culture room and the control panel 7 of the preservation room are provided with switches for controlling the ultraviolet lamp 12, the fluorescent lamp 13 and the semiconductor refrigerating device 14, and a temperature-controlled electromagnetic relay.
Example 2
The embodiment provides a working principle and a using method of an isolated pollen culture and preservation box for wheat crops, which comprises the following steps:
(1) the interiors of the culture chamber 1 and the preservation chamber 2 are cleaned and sterilized with alcohol.
(2) The battery 8 is fully charged by adopting a charger, and when the electric quantity is insufficient under outdoor conditions, the solar cell panel 9 can be unfolded at a proper angle to carry out solar charging.
(3) The detachable test-tube rack 16 of suitable size is placed on the test-tube rack support 15, the test tube is placed in, the test tube contains the anther culture medium, the ultraviolet lamp 12 is started to sterilize the culture room 1, and the residual pollen grains are killed.
(4) Before hybridization, the anthers are collected and placed in a test tube in a storage room 2, and the anthers are stored at a low temperature of 4 ℃ to stop the development of the anthers.
(5) During the hybridization operation, the anthers in the storage chamber 2 are transferred to the test tubes in the culture chamber 1, and the anthers are cultured at a suitable temperature.
The foregoing is directed to embodiments of the present application and it is noted that numerous modifications and adaptations may be made by those skilled in the art without departing from the principles of the present application and are intended to be within the scope of the present application.

Claims (8)

1. The utility model provides a wheat class crop anther is kept in vitro and incubator which characterized in that structure includes: cultivate room and save the room, cultivate the room and keep the room and separate by the heat insulating board, it is equipped with the observation window to cultivate the room top, it is equipped with the chamber door and can open through handle and loose-leaf to cultivate room and save the room top, it is equipped with respectively to cultivate room control panel and save room control panel with the save room to cultivate room, lithium cell and solar cell panel provide the electric energy for ultraviolet lamp, fluorescent lamp and semiconductor refrigerating plant, it is equipped with the test-tube rack support in room and the save room to cultivate the room, the test-tube rack can be fixed to the test-tube rack support.
2. The isolated preservation and cultivation box for wheat and wheat crops anther as claimed in claim 1, wherein the box body and the box door of the cultivation room and the preservation room are formed by processing high-quality cold-rolled steel plates, and the observation window of the cultivation room is made of double-layer hollow glass to ensure the heat preservation performance of the cultivation room.
3. The isolated storage and culture box for wheat crop anthers as claimed in claim 1, wherein the doors of the culture room and the preservation room are provided with seals, and the seals are made of polyvinyl chloride.
4. The isolated preservation and culture box for wheat crop anthers as claimed in claim 1, wherein the culture chamber adopts a mirror stainless steel inner container with four corners and a semicircular arc transition; the heat-insulating layer of the preservation chamber is made of a vacuum heat-insulating plate.
5. The isolated preservation and culture box for wheat crop anther as claimed in claim 1, wherein the main component of the temperature control system of the culture chamber and the preservation chamber is a semiconductor refrigeration sheet.
6. The isolated storage and culture box for wheat crop anthers as claimed in claim 1, wherein the culture chamber and the storage chamber are respectively provided with independent electromagnetic relay temperature control systems.
7. The isolated storage and culture box for wheat crop anthers as claimed in claim 1, wherein the detachable test tube rack is made of stainless steel, and the square of the test tube rack is detachable and adjustable.
8. The isolated storage and culture box for wheat crop anthers as claimed in claim 1, wherein the lithium battery and the solar panel supply power to ultraviolet lamps, fluorescent lamps and semiconductor refrigeration devices.
CN202121052766.6U 2021-05-17 2021-05-17 Wheat crop anther in-vitro preservation and culture box Active CN214961710U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121052766.6U CN214961710U (en) 2021-05-17 2021-05-17 Wheat crop anther in-vitro preservation and culture box

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121052766.6U CN214961710U (en) 2021-05-17 2021-05-17 Wheat crop anther in-vitro preservation and culture box

Publications (1)

Publication Number Publication Date
CN214961710U true CN214961710U (en) 2021-12-03

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ID=79131774

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121052766.6U Active CN214961710U (en) 2021-05-17 2021-05-17 Wheat crop anther in-vitro preservation and culture box

Country Status (1)

Country Link
CN (1) CN214961710U (en)

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