CN214953067U

CN214953067U - Microorganism film reading system

Info

Publication number: CN214953067U
Application number: CN202121004165.8U
Authority: CN
Inventors: 陈涛; 张静; 林金飞; 任静; 严喜平; 黎俊杰; 钟华强; 朱俊玲
Original assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Current assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Priority date: 2021-05-11
Filing date: 2021-05-11
Publication date: 2021-11-30
Anticipated expiration: 2031-05-11

Abstract

The utility model discloses a microorganism film reading system, which comprises a film reading system and an industrial control host, wherein the film reading system comprises a light source, an objective table, an objective lens, a dichroic mirror, an excitation device, a blocking filter and a light field lens; when the light source is started, light rays emitted by the light source sequentially pass through the objective table and the objective lens to enter the light field lens; when the excitation device is started, excitation light emitted by the excitation device sequentially enters the light field lens through the dichroic mirror, the objective table, the objective lens, the dichroic mirror and the blocking filter; the industrial control host is used for controlling the on-off of the light source and the excitation device. The utility model adopts the industrial control host to record the film reading result, thereby realizing the film reading function; moreover, the light field lens is adopted for shooting, so that the focusing time can be saved; and the industrial control host can control the on-off of the light source and the excitation device, and different film reading modes can be adopted according to actual needs. The utility model relates to a medical science inspection field.

Description

Microorganism film reading system

Technical Field

The utility model relates to a piece system is read to microorganism in the medical science inspection field.

Background

In medical testing, it is often necessary to perform a reading test on a microbiological sample. For example, in the diagnosis of pulmonary tuberculosis, sputum smear direct microscopic examination is an important detection and diagnosis means for pulmonary tuberculosis, and the manual microscopic examination has the problems of high labor intensity of detection personnel, obvious difference of working years and experience of the personnel, difficulty in improving the detection efficiency and accuracy and the like, and has iatrogenic infection risk in the manual detection process.

Moreover, when the slide reading inspection is carried out, because the microscopic surface of the sample is uneven, a plurality of focusing shots are often required to obtain a high-definition image, and a large number of intermediate files are generated, so that the inspection process is complicated and the efficiency is low.

In addition, the sputum smear has two staining modes of acid-fast staining and fluorescence staining, and requires bright-field film reading and dark-field fluorescence excitation film reading respectively, the current film reading system can only complete one of the film reading modes, and the switching of the film reading mode is very inconvenient.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to solve one of the technical problem that exists among the prior art at least, provide a piece system is read to microorganism, can save the time of focusing and can switch and read the piece mode.

According to the embodiment of the utility model, a microorganism film reading system is provided, including film reading system and industrial control host computer, film reading system includes light source, objective table, objective, dichroic mirror, excitation device, blocking filter and light field camera lens; when the light source is turned on: light rays emitted by the light source irradiate the objective table, penetrate through the objective table and then enter the light field lens through the objective lens; when the excitation device is started: exciting light emitted by the excitation device is reflected to the objective table through the dichroic mirror, part of the exciting light is reflected by the objective table, and enters the light field lens through the objective lens, the dichroic mirror and the blocking filter in sequence; the industrial personal computer is electrically connected with the light source and the excitation device and is used for controlling the on-off of the light source and the excitation device.

According to the embodiment of the utility model provides a, furtherly, read piece system still includes conversion equipment, the dichroic mirror with block the filter all with conversion equipment connects, conversion equipment is used for driving the dichroic mirror with block the filter and remove at the horizontal direction.

According to the utility model provides a, furtherly, the light source is the LED light source.

According to the embodiment of the utility model provides a, furtherly, industry control host computer with the light field camera lens electricity is connected.

According to the utility model discloses embodiment, furtherly, the objective table can remove along the horizontal direction.

According to the embodiment of the present invention, further, the quantity of objective is at least two, the film reading system further includes a lens converter, each the objective all with the lens converter is connected, the lens converter is used for switching the objective, so that each the objective respectively with the light source aligns.

According to the utility model discloses, furtherly, excitation device includes excitation light source and excitation filter, excitation light source sends the exciting light, the exciting light passes through the excitation filter reachs the dichroic mirror.

According to the embodiment of the utility model provides a, furtherly, the optical axis of the light that excitation device sent is first optical axis, the optical axis of the light that the light source sent is the second optical axis, the second optical axis is perpendicular with the horizontal direction, first optical axis with second optical axis nonparallel just has the nodical point, the dichroic mirror is located first optical axis with the nodical point department of second optical axis, the mirror surface of dichroic mirror with first optical axis the contained angle of second optical axis equals.

According to the embodiment of the utility model provides a, furtherly, the mirror surface of dichroic mirror with first optical axis the contained angle of second optical axis is 45 degrees.

According to the utility model discloses, furtherly, the piece system is read to microorganism still includes the bar code scanner, the bar code scanner with industry control host computer electricity is connected, the bar code scanner is used for discerning the sample serial number.

The utility model has the advantages that: the utility model adopts the industrial control host to record the film reading result, thereby realizing the film reading function; moreover, the light field lens is adopted for shooting, so that the focusing time can be saved; and the industrial control host can control the on-off of the light source and the excitation device, and different film reading modes can be adopted according to actual needs.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures represent only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from these figures without inventive effort.

Fig. 1 is a schematic diagram of the present invention.

Detailed Description

This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means are one or more, a plurality of means are two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the number, and the terms greater than, less than, within, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

Referring to fig. 1, the utility model discloses a slide system is read to microorganism includes reads slide system and industrial control host computer, wherein reads slide system and includes light source 1, objective table 2, objective 3, dichroic mirror 4, excitation device 5, blocks filter 6 and light field camera lens 7. The film reading system comprises a bright field mode and a fluorescence mode, when the bright field mode is used for reading the film, the light source 1 is started, the excitation device 5 is closed, light rays emitted by the light source 1 firstly irradiate the objective table 2, penetrate through the objective table 2 and then enter the light field lens 7 through the objective lens 3, and therefore the film reading is completed. When the fluorescence mode is used for reading the film, the excitation device 5 is turned on, the light source 1 is turned off, the excitation light is emitted from the excitation device 5 and reflected to the objective table 2 through the dichroic mirror 4, then part of the excitation light is absorbed by the sample, and the rest of the excitation light is reflected by the objective table 2 and enters the light field lens 7 through the objective lens 3, the dichroic mirror 4 and the blocking filter 6 in sequence, so that the film reading is completed.

Specifically, the light field lens 7 can capture light rays from all directions in a scene, and can perform focusing processing by using a computer after shooting is completed without focusing in the shooting process. The method only needs to take one picture in each visual field, greatly saves the time required by focusing and improves the film reading efficiency.

Further, the slide reading system further comprises a conversion device, the dichroic mirror 4 and the blocking filter 6 are both mounted on the conversion device, the conversion device can drive the dichroic mirror 4 and the blocking filter 6 to move horizontally relative to the stage, and in this application, as shown in fig. 1, the horizontal direction refers to a direction parallel to the bearing surface of the stage 2. In the present embodiment, the conversion device is horizontally rotatable, the dichroic mirror 4 and the blocking filter 6 are both mounted on the peripheral side of the conversion device, and the dichroic mirror 4 is located directly above the blocking filter 6. When the bright field mode is used for reading the film, the conversion device moves the dichroic mirror 4 and the blocking filter 6 out of the optical path to prevent the imaging effect from being influenced; when fluorescence mode reading is used, the conversion device moves the dichroic mirror 4 and the blocking filter 6 into the optical path of the light excited by the excitation device 5, so that the light can finally enter the light field lens 7. It is understood that other devices or structures capable of achieving the same function may be adopted in the conversion device, and are not described in detail herein.

Further, the industrial control host is electrically connected with the light source 1 and the excitation device 5, so that opening and closing of the light source 1 and the excitation device 5 can be controlled, and switching of the film reading mode is achieved. The industrial control host is also electrically connected with the optical field lens 7 and is used for reading images shot by the optical field lens 7, so that the film reading efficiency is improved.

Further, the power source 1 is an LED light source. The LED light source has long service life, the color temperature is almost unchanged after aging, and the LED light source is suitable for quantitative evaluation and reduces the maintenance frequency of equipment.

Further, the stage 2 can be moved in the horizontal direction by a jack screw mechanism for adjusting the observation position of the sample. Other devices or mechanisms that perform the same function may be used and will not be described further herein.

Further, the number of the objective lenses 3 is at least two, and the film reading system further comprises a lens converter, wherein the lens converter can be used for installing a plurality of objective lenses 3 with different specifications. In this application, the objective lens 3 being aligned with the light source means that the second optical axis of the light source can extend through the objective lens 3. The lens converter can drive each objective lens 3 to move horizontally relative to the objective table, in the embodiment, the lens converter can rotate horizontally, each objective lens 3 is installed on the periphery of the lens converter and used for switching the objective lenses 3 according to requirements, and the lens converter does not need to adjust the distance between the objective lenses and the objective table because the light field lenses 7 do not need to be focused, so that the film reading efficiency can be improved. It is to be understood that other devices or structures capable of implementing the same function may be adopted in the lens converter, and are not described herein again.

Further, the excitation device 5 includes an excitation light source 51 and an excitation filter 52, and after the excitation light source 51 emits excitation light, the excitation light reaches the dichroic mirror through the excitation filter 52. The excitation filter 52 is used for screening excitation light of an optimal waveband for the fluorescent dye of the detection sample, and can absorb a long-wave spectral line and enable purple, blue and green light rays and ultraviolet rays to pass through, so that the imaging quality of a fluorescence film reading mode is improved.

Furthermore, the excitation light source 51 is a metal halogen lamp, and the wavelength coverage range is 350nm to 700nm, so that the system can be used for various fluorescent pigments, and the applicability of the system is improved. It is understood that the excitation light source 51 may be replaced by other excitation light sources to enhance the applicability to different use cases.

Further, the optical axis of the excitation light emitted by the excitation device 5 is a first optical axis, the optical axis of the light emitted by the light source 1 is a second optical axis, the second optical axis is perpendicular to the horizontal direction, and the first optical axis and the second optical axis are not parallel but intersect. The dichroic mirror 4 is located at the intersection of the first optical axis and the second optical axis, the dichroic mirror 4 is installed obliquely, and the included angle between the mirror surface of the dichroic mirror 4 and the first optical axis is equal to the included angle between the mirror surface of the dichroic mirror 4 and the second optical axis. The optical axes in the two modes are not parallel, so that the two modes can be switched conveniently. The dichroic mirror 4 is used for reflecting the excitation light onto the sample to be detected, exciting the sample to emit fluorescence, and transmitting the reflected fluorescence to the blocking filter 6 and the light field lens 7.

Furthermore, the included angles between the mirror surface of the dichroic mirror 4 and the first optical axis and between the mirror surface of the dichroic mirror 4 and the second optical axis are both 45 degrees, which is convenient for the installation and debugging of the dichroic mirror 4.

Further, the microorganism slide reading system also comprises a bar code scanner 8, wherein the bar code scanner 8 is electrically connected with the industrial control host and is used for identifying the sample number on the sample culture medium, and the sample number adopts a two-dimensional code form and can also adopt other forms which are convenient to scan. After the bar code scanner 8 finishes scanning, the number information is sent to the industrial personal computer, so that later filing processing is facilitated.

While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the invention is not limited to the details of the embodiments shown, but is capable of various modifications and substitutions without departing from the spirit of the invention.

Claims

1. A microorganism film reading system is characterized by comprising a film reading system and an industrial control host,

the film reading system comprises: the device comprises a light source (1), an objective table (2), an objective lens (3), a dichroic mirror (4), an excitation device (5), a blocking filter (6) and a light field lens (7);

when the light source (1) is turned on: light rays emitted by the light source (1) irradiate the object stage (2), penetrate through the object stage (2) and then enter the light field lens (7) through the objective lens (3);

when the excitation device (5) is turned on: the exciting light emitted by the exciting device (5) is reflected to the objective table (2) through the dichroic mirror (4), part of the exciting light is reflected by the objective table (2), and enters the light field lens (7) through the objective lens (3), the dichroic mirror (4) and the blocking filter (6) in sequence;

the industrial control host is electrically connected with the light source (1) and the excitation device (5), and is used for controlling the opening and closing of the light source (1) and the excitation device (5).

2. The microbial reading system of claim 1, wherein: read piece system still includes conversion equipment, dichroic mirror (4) with block filter (6) all with conversion equipment connects, conversion equipment is used for driving dichroic mirror (4) with block filter (6) and remove in the horizontal direction.

3. The microbial reading system of claim 1, wherein: the light source (1) is an LED light source.

4. The microbial reading system of claim 1, wherein: the industrial control host is electrically connected with the optical field lens (7).

5. The microbial reading system of claim 1, wherein: the object stage (2) can move along the horizontal direction.

6. The microbial reading system of claim 1, wherein: the number of the objective lenses (3) is at least two, the film reading system further comprises a lens converter, each objective lens (3) is connected with the lens converter, and the lens converter is used for switching the objective lenses (3) so that each objective lens (3) is aligned with the light source.

7. The microbial reading system of claim 1, wherein: the excitation device (5) comprises an excitation light source (51) and an excitation filter (52), wherein the excitation light source (51) emits excitation light, and the excitation light reaches the dichroic mirror (4) through the excitation filter (52).

8. The microbial reading system of claim 1, wherein: the optical axis of light that excitation device (5) sent is first optical axis, the optical axis of light that light source (1) sent is the second optical axis, the second optical axis is perpendicular with the horizontal direction, first optical axis with second optical axis nonparallel just has the nodical, dichroic mirror (4) are located first optical axis with the intersection point department of second optical axis, the mirror surface of dichroic mirror (4) with first optical axis the contained angle of second optical axis equals.

9. The microbial reading system of claim 8, wherein: the mirror surface of dichroic mirror (4) with first optical axis, the contained angle of second optical axis is 45 degrees.

10. The microbial reading system of claim 1, wherein: the microorganism film reading system further comprises a bar code scanner (8), the bar code scanner (8) is electrically connected with the industrial control host, and the bar code scanner (8) is used for identifying a sample number.