CN208921606U - Fluorescence detection microscope - Google Patents
Fluorescence detection microscope Download PDFInfo
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- CN208921606U CN208921606U CN201821512745.6U CN201821512745U CN208921606U CN 208921606 U CN208921606 U CN 208921606U CN 201821512745 U CN201821512745 U CN 201821512745U CN 208921606 U CN208921606 U CN 208921606U
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- guide plate
- fluorescence detection
- mirror holder
- objective table
- pedestal
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Abstract
The utility model relates to a kind of fluorescence detection microscopes comprising mirror holder, objective table, eyepiece and object lens, exposure light source, scanning lens and processor.The fluorescence detection microscopic structure is relatively easy, easy to operate, is applicable to CTC detection imaging, has many advantages, such as that testing cost is low, detection accuracy, can be widely used to promote.Further, the microscopical objective table of the fluorescence detection can move in three-dimensional space, and adjustment structure deft design, easy to adjust, according to detection needs, can easily adjust the position for the sample to be tested being placed on it.
Description
Technical field
The utility model relates to a kind of fluorescence detection microscopes.
Background technique
Circulating tumor cell (Circulating Tumor Cell, CTC) refer to it is spontaneous or because operation of diagnosis and treatment from tumour original
Hair stove or metastatic lesion shed into the tumour cell of Peripheral Circulation system.Though CTC quantity is few in tumor patient blood,
Tumour real time status can be presented in it.It is existing free single a large number of studies show that CTC is present in peripheral blood with different shape
CTC also has the pockets of cell mass (Circulating Tumor Microemboli, CTM) of aggregation.Tumour cell is outside entering
Epithelial-mesenchymal transformation (Epithelial Mesenchymal Transition, EMT) can occur during all blood circulations, therefore
There are different types, including epithelial cell phenotype, interstitial cell phenotype and epithelial cell to mix phenotype with interstitial cell by CTC.CTM
There is stronger metastatic potential with interstitial cell phenotype CTC.
, there are many methods for identifying CTC in being constantly progressive and develop with science and technology, and fluorescence has in CTC identification
Be widely applied.Fluorescence mainly passes through the aptamers knot such as fluorophor and other antibody, probe in CTC identification at present
Close, serve when observing CTC it is indicative, more intuitively to observe or count CTC.But most of fluorophors are easy to appear
Fluorescent quenching, therefore while guaranteeing to be protected from light operation, it is necessary to the suitable adaptation bulk concentration of selection.Meanwhile probe or antibody
Tumour-specific also will affect qualification result, ensure that detection reliability while, but also this method can only be used
In specific cell detection, the scope of application is limited.
Fluorescence primarily serves indicative effect in the detection of CTC, specific fluorophor is used to carry out sample special
Different label, sample are illuminated and thus detected by the fluorescent x ray detector appropriate that excitation generates by excitation rays.Pass through
This mode can show each cellular portions differently dyed in fluorescence microscope.Spy can certainly be passed through simultaneously
Surely the different pigments accumulated on the different structure of sample dye the multiple portions of sample, consequently facilitating intuitively seeing
Examine, identify and count CTC.Now, novel probe technology emerges one after another, and utilizes the light of biological fluorescent labeling and Laser Scanning Confocal Microscope
Slide imaging technologies rapid dyeing and image tissue cell are learned, marks the cytoplasm of cell respectively using a variety of biological fluorescent labelings
It with other organelles, and is imaged respectively under the laser excitation of different wave length, image is combined and is decomposed on computers.
This method is dyed relative to HE, be imaged it is apparent, more rapidly, it is simpler, be more suitable for large-scale application popularization.However it is traditional
The detection systems somewhat expensive such as Laser Scanning Confocal Microscope, testing cost are high.
Utility model content
Based on this, it is necessary to it is aobvious to provide a kind of fluorescence detection for advantageously reducing testing cost, detecting imaging suitable for CTC
Micro mirror.
A kind of fluorescence detection microscope, comprising:
Mirror holder；
Objective table is set on the mirror holder, and the objective table can move in three-dimensional space relative to the mirror holder, with
For adjusting the position of sample to be tested；
Eyepiece and object lens are set on the mirror holder, and are located at the side of the objective table, and the object lens are toward the load
The setting of object platform；
Exposure light source is set on the mirror holder, and is located at the other side of the objective table, and the exposure light source is for irradiating
The sample to be tested on the objective table is placed in excite generation fluorescence；
Scanning lens and processor, the scanning lens are set on the mirror holder to be located at the objective table for scanning
On sample to be tested, and scanning result is sent to the processor, the processor carries out imaging to scanning result.
The objective table includes pedestal, the first guide plate, the second guide plate and loading in one of the embodiments,
Plate；The pedestal is flexibly connected with the mirror holder and can rise or fall relative to the mirror holder；First guide plate, institute
It states the second guide plate and the support board is cascadingly set on the pedestal, wherein second guide plate and described the
One guide plate is flexibly connected and second guide plate can move in the y-direction relative to first guide plate, the loading
Plate is flexibly connected with second guide plate and the support board can move in the x-direction relative to second guide plate.
It is connected between the pedestal and the mirror holder by drive lead screw in one of the embodiments, on the mirror holder
Equipped with the first adjusting knob, first adjusting knob is connect for adjusting the pedestal in the mirror with the drive lead screw
Height on frame.
First guide plate can be slided with second guide plate by sliding block sliding groove structure in one of the embodiments,
Dynamic connection.
First guide plate is equipped with the second adjusting knob, second adjusting knob in one of the embodiments,
Be fixedly connected with second guide plate, by rotate second adjusting knob be able to drive second guide plate relative to
The first guide plate movement.
Second guide plate is slidably connected with the support board by sliding block sliding groove structure in one of the embodiments,
It connects.
Second guide plate is equipped with third adjusting knob, the third adjusting knob in one of the embodiments,
It is fixedly connected with the support board, is able to drive the support board relative to described second by rotating the third adjusting knob
Guide plate movement.
The fluorescence detection microscope further includes concentrating component in one of the embodiments, and the exposure light source issues
Exciting light be radiated at after the concentrating component on the objective table.
The concentrating component is set on the pedestal in one of the embodiments, the concentrating component and the pedestal
It is connected by drive lead screw, the pedestal is equipped with the 4th adjusting knob that connect with the drive lead screw, passes through rotation described the
Four adjusting knobs are able to drive the concentrating component and rise or fall relative to the pedestal.
The fluorescence detection microscope further includes the 5th adjusting knob in one of the embodiments, and the described 5th is adjusted
Knob is used to adjust the scan position of the scanning lens.
Above-mentioned fluorescence detection microscopic structure is relatively easy, easy to operate, is applicable to CTC detection imaging, has detection
It the advantages that at low cost, detection accuracy, can be widely used to promote.
Further, the microscopical objective table of the fluorescence detection can move in three-dimensional space, and adjustment structure designs
Exquisiteness, it is easy to adjust, according to detection needs, it can easily adjust the position for the sample to be tested being placed on it.
Detailed description of the invention
Fig. 1 is the microscopical structural schematic diagram of fluorescence detection of an embodiment of the present invention；
Fig. 2 and Fig. 3 is respectively the structural schematic diagram at other microscopical visual angles of fluorescence detection shown in Fig. 1.
Description of symbols:
10: fluorescence detection microscope；100: mirror holder；110: microscope base；120: handel；122 the 5th adjusting knobs；200: loading
Platform；202: sample deposition；204: window；210: pedestal；220: the first guide plates；230: the second guide plates；240: support board；
250: the first adjusting knobs；260: the second adjusting knobs: 270: third adjusting knob；222 and 232: fixture nut；300: eyepiece
Component；310: eyepiece stalk；320: eyepiece；400: objective lens unit；410: Nosepiece；420: object lens；500: exposure light source；
600: scanning lens；700: processor；800: controller；900: concentrating component；910: the four adjusting knobs.
Specific embodiment
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms in fact
It is existing, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes public affairs to the utility model
The understanding for opening content is more thorough and comprehensive.
It should be noted that be referred to as " being set to " another element when element, it can directly on the other element or
There may also be elements placed in the middle by person.When an element is considered as " connection " another element, it can be directly to
Another element may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
Any and all combinations of one or more related listed items.
Incorporated by reference to FIG. 1 to FIG. 3, an embodiment of the present invention provides a kind of fluorescence detection microscope 10 comprising mirror
Frame 100, objective table 200, eyepiece component 300, objective lens unit 400, exposure light source 500, scanning lens 600 and processor 700.
Mirror holder 100 includes microscope base 110 and handel 120.Handel 120 is set on microscope base 110.Handel 120 is substantially L-shaped.?
In one specific example, microscope base 110 and handel 120 can be integrally formed structure.
Objective table 200 is set on mirror holder 100, with one for placing the sample deposition 202 of sample to be tested.Objective table
The position of 200 counter sample rest areas 202 is equipped with window 204 so that exciting light passes through.Objective table 200 can be relative to mirror holder
100 move in three-dimensional space, with the position for adjusting sample to be tested.
In the specific example of diagram, objective table 200 include pedestal 210, the first guide plate 220, the second guide plate 230 with
And support board 240.Pedestal 210 is flexibly connected with handel 120 and can rise or fall relative to handel 120.First guide plate
220, the second guide plate 230 and support board 240 are cascadingly set on pedestal 210.Wherein, support board 240 is equipped with above-mentioned
Sample deposition 202, the window 204 that the first guide plate 220, the second guide plate 230 and support board 240 are disposed through.Second
Guide plate 230 is flexibly connected with the first guide plate 220 and the second guide plate 230 can be relative to the first guide plate 220 in the y-direction
Movement, support board 240 is flexibly connected with the second guide plate 230 and support board 240 can be relative to the second guide plate 230 along the side x
To movement." direction x " and " direction y " as described herein refer to that any two in the plane perpendicular to vertical direction are mutually hung down
Straight linear direction.
More specifically, being connected between pedestal 210 and handel 120 by drive lead screw (not shown).Handel 120 is equipped with the
One adjusting knob 250.First adjusting knob 250 is connect with drive lead screw.Platform is able to drive by rotating the first adjusting knob 250
Seat 210 rises or falls, and then support board 240 is driven to rise or fall, for adjusting support board 240 on mirror holder 100
Highly.
First guide plate 220 and the second guide plate 230 pass through sliding block sliding groove structure slidable connection.In diagrammatically shown tool
In body example, the first guide plate 220 is equipped with sliding slot, and the second guide plate 230 is equipped with towards the side of the first guide plate 220 and slides
Block, sliding block, which is caught in the sliding slot, makes the first guide plate 220 be slidably connected with the second guide plate 230.It is understood that specific at other
In example, sliding slot can also be set on the second guide plate 230, sliding block is set on the first guide plate 220；Or it is led first
Sliding block and sliding slot are set simultaneously on plate 220 and the second guide plate 230.
Further, the first guide plate 220 is equipped with the second adjusting knob 260.Specifically, the first guide plate 220 is equipped with
Fixture nut 222, the second adjusting knob 260 are threadedly coupled with fixture nut 222.Second adjusting knob 260 and the second guide plate
230 are fixedly connected, and are able to drive the second guide plate 230 by the second adjusting knob 260 of rotation and transport relative to the first guide plate 220
It is dynamic.
Second guide plate 230 passes through sliding block sliding groove structure slidable connection with support board 240.Specifically show in diagrammatically shown
In example, the second guide plate 230 is equipped with sliding slot, and support board 240 is equipped with sliding block, sliding block card towards the side of the second guide plate 230
Entering makes the second guide plate 230 be slidably connected with support board 240 in the sliding slot.It, can also be with it is understood that in other specific examples
Sliding slot is set on support board 240, sliding block is set on the second guide plate 230；Or in the second guide plate 230 and support board
Sliding block and sliding slot are set simultaneously on 240.
Further, the second guide plate 230 is equipped with third adjusting knob 270.Specifically, the second guide plate 230 is equipped with
Fixture nut 232, third adjusting knob 270 are threadedly coupled with fixture nut 232.Third adjusting knob 270 and the second guide plate
230 are fixedly connected, and are able to drive the second guide plate 230 by rotation third adjusting knob 270 and transport relative to the second guide plate 230
It is dynamic.
It can be adjusted by the first adjusting knob 250 of rotation, the second adjusting knob 260 and/or third adjusting knob 270
Position of the sample to be tested on support board 240, and then can choose suitable viewing area and carry out observation or scanning imagery.
Eyepiece component 300 and objective lens unit 400 are located at the side of objective table 200, and eyepiece component 300 is towards objective table
200 settings.
Eyepiece component 300 includes eyepiece stalk 310 and eyepiece 320.Eyepiece stalk 310 is mounted on handel 120, it is preferred that
Eyepiece stalk 310 is rotatably connected with handel 120.Eyepiece 320 is mounted on eyepiece stalk 310.In diagrammatically shown specific example,
Eyepiece component 300 includes two eyepieces 320, is binocular system.Adjustable angle between two eyepieces 320.Times of eyepiece 320
Number can be but not limited to 10 times of mirrors, and visual field can be but not limited to 25mm.
Objective lens unit 400 includes Nosepiece 410 and object lens 420.Nosepiece 410 is pivotally mounted to handel
On 120.Nosepiece 410 have multiple object lens installation positions, with for install have different multiples (such as 10 ×, 20 ×, 40 ×
Deng) object lens 420.The preferred enhanced contrast type flat field fluorescence object lens of object lens 420.
Exposure light source 500 is set on mirror holder 100, and is located at the other side of objective table 200, and exposure light source 500 is for irradiating
The sample to be tested on objective table 200 is placed in excite generation fluorescence.
Preferably, which further includes controller 800.Controller 800 electrically connects with exposure light source 500
It connects, with the time for exposure for controlling exposure light source 500.For example, controller 800 is exposed including feux rouges in a specific example
Light time control module, green light time for exposure control module, orange light time for exposure control module and blue light time for exposure control mould
Block, the time for exposure that the feux rouges time for exposure control module is set as control feux rouges adjust automatic exposure, maximum exposure time
It can be but not limited to 2.0s；The time for exposure that the green light time for exposure control module is set as control green light adjusts automatic expose
Light, maximum exposure time can be but not limited to 2.0s；The orange light time for exposure control module be set as control orange light when
Between adjust automatic exposure, maximum exposure time can be but not limited to 2.0s；The blue light time for exposure control module is set as
The time for exposure for controlling blue light adjusts automatic exposure, and maximum exposure time can be but not limited to 1.0s.
In addition, controller 800 can also control be irradiated to it is photochromic on sample to be tested, such as can by control one have it is more
The turntable rotation of a optical filter is photochromic to adjust, and multiple optical filters of turntable have different colors, (thoroughly if any Red lightscreening plate
Cross wavelength 616-649nm), blue color filter (through wavelength 330-385nm), orange optical filter (through wavelength 545-580nm)
With green color filter (penetrate wavelength 460-495nm) etc..
Scanning lens 600 be set to mirror holder 100 on for scan be located at objective table 200 on sample to be tested, and will scan
As a result it is sent to processor 700, processor 700 carries out imaging to scanning result.Scanning lens 600 can be but not limited to
CCD camera lens.
Further, in diagrammatically shown specific example, which further includes concentrating component 900.It exposes
The exciting light that radiant 500 issues is radiated on objective table 200 after concentrating component 900.
Concentrating component 900 is set on pedestal 210.Concentrating component 900 is connect with pedestal 210 by drive lead screw, pedestal 210
It is equipped with the 4th adjusting knob 910 connecting with the drive lead screw, is able to drive optically focused group by rotating the 4th adjusting knob 910
Part 900 rises or falls relative to pedestal 210.
In addition, fluorescence detection microscope 10 further includes the 5th adjusting knob 122, the 5th adjusting knob 122 is swept for adjusting
Retouch the scan position of camera lens 600.
Above-mentioned 10 structure of fluorescence detection microscope is relatively easy, easy to operate, is applicable to CTC detection imaging, has inspection
The advantages that surveying at low cost, detection accuracy, can be widely used to promote.
Further, the objective table 200 of the fluorescence detection microscope 10 can move in three-dimensional space, and adjustment structure
Deft design, it is easy to adjust, according to detection needs, it can easily adjust the position for the sample to be tested being placed on it.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (10)
1. a kind of fluorescence detection microscope characterized by comprising
Mirror holder；
Objective table is set on the mirror holder, and the objective table can move in three-dimensional space relative to the mirror holder, to be used for
Adjust the position of sample to be tested；
Eyepiece and object lens are set on the mirror holder, and are located at the side of the objective table, and the object lens are toward the objective table
Setting；
Exposure light source is set on the mirror holder, and is located at the other side of the objective table, and the exposure light source is placed for irradiating
In the sample to be tested on the objective table to excite generation fluorescence；
Scanning lens and processor, the scanning lens are set on the mirror holder to be located on the objective table for scanning
Sample to be tested, and scanning result is sent to the processor, the processor carries out imaging to scanning result.
2. fluorescence detection microscope as described in claim 1, which is characterized in that the objective table includes pedestal, the first guiding
Plate, the second guide plate and support board；The pedestal be flexibly connected with the mirror holder and can relative to the mirror holder rise or
Decline；First guide plate, second guide plate and the support board are cascadingly set on the pedestal, wherein
Second guide plate is flexibly connected with first guide plate and second guide plate can be relative to first guiding
Plate moves in the y-direction, and the support board is flexibly connected with second guide plate and the support board can be relative to described
Two guide plates move in the x-direction.
3. fluorescence detection microscope as claimed in claim 2, which is characterized in that pass through biography between the pedestal and the mirror holder
Dynamic screw rod connection, the mirror holder are equipped with the first adjusting knob, first adjusting knob connect with the drive lead screw with
In height of the adjusting pedestal on the mirror holder.
4. fluorescence detection microscope as claimed in claim 2, which is characterized in that first guide plate and second guiding
Plate passes through sliding block sliding groove structure slidable connection.
5. fluorescence detection microscope as claimed in claim 4, which is characterized in that first guide plate is equipped with second and adjusts
Knob, second adjusting knob are fixedly connected with second guide plate, being capable of band by rotating second adjusting knob
Second guide plate is moved to move relative to first guide plate.
6. fluorescence detection microscope as claimed in claim 2, which is characterized in that second guide plate and the support board are logical
Cross sliding block sliding groove structure slidable connection.
7. fluorescence detection microscope as claimed in claim 6, which is characterized in that second guide plate is adjusted equipped with third
Knob, the third adjusting knob are fixedly connected with the support board, are able to drive institute by rotating the third adjusting knob
Support board is stated to move relative to second guide plate.
8. the fluorescence detection microscope as described in any one of claim 2~7, which is characterized in that it further include concentrating component, institute
The exciting light for stating exposure light source sending is radiated on the objective table after the concentrating component.
9. fluorescence detection microscope as claimed in claim 8, which is characterized in that the concentrating component is set on the pedestal,
The concentrating component is connect with the pedestal by drive lead screw, and the pedestal is equipped with the 4th connect with the drive lead screw and adjusts
Knob is saved, the concentrating component is able to drive by rotation the 4th adjusting knob and is risen or fallen relative to the pedestal.
10. such as fluorescence detection microscope according to any one of claims 1 to 7, which is characterized in that further include the 5th adjusting rotation
Button, the 5th adjusting knob are used to adjust the scan position of the scanning lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821512745.6U CN208921606U (en) | 2018-09-14 | 2018-09-14 | Fluorescence detection microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821512745.6U CN208921606U (en) | 2018-09-14 | 2018-09-14 | Fluorescence detection microscope |
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| Publication Number | Publication Date |
|---|---|
| CN208921606U true CN208921606U (en) | 2019-05-31 |
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ID=66705793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821512745.6U Active CN208921606U (en) | 2018-09-14 | 2018-09-14 | Fluorescence detection microscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456496A (en) * | 2019-07-11 | 2019-11-15 | 亳州职业技术学院 | A kind of adjustable microscope |
| CN114779460A (en) * | 2022-04-28 | 2022-07-22 | 中国人民解放军总医院第三医学中心 | Portable biological microscope |
-
2018
- 2018-09-14 CN CN201821512745.6U patent/CN208921606U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110456496A (en) * | 2019-07-11 | 2019-11-15 | 亳州职业技术学院 | A kind of adjustable microscope |
| CN110456496B (en) * | 2019-07-11 | 2021-05-28 | 亳州职业技术学院 | Adjustable microscope |
| CN114779460A (en) * | 2022-04-28 | 2022-07-22 | 中国人民解放军总医院第三医学中心 | Portable biological microscope |
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