CN214794465U - High-sensitivity marker excitation and detection structure - Google Patents
High-sensitivity marker excitation and detection structure Download PDFInfo
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- CN214794465U CN214794465U CN202120651626.4U CN202120651626U CN214794465U CN 214794465 U CN214794465 U CN 214794465U CN 202120651626 U CN202120651626 U CN 202120651626U CN 214794465 U CN214794465 U CN 214794465U
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Abstract
The utility model discloses a high sensitivity marker arouses and detects structure, including light source, reflector plate and image acquisition device. The light emitted by the light source comprises marker excitation light, the reflecting lens reflects the marker excitation light to the object to be detected, and the light emitted by the light source except the marker excitation light penetrates through the reflecting lens, so that noise and light interference generated by reflecting stray light to the object to be detected is avoided; then wait to detect the thing and send marker response light, marker response light propagates along the reverse extending direction of the light path direction of marker exciting light, because the wavelength of marker response light is different with marker exciting light, behind the marker response light sees through the reflection lens, is caught by image acquisition device and acquires, and image acquisition device obtains the image that contains marker response light spot quantity and distribution position to supply computer or manual work to carry out high accuracy ground analysis, the utility model discloses still have simple structure, characteristics with low costs.
Description
Technical Field
The utility model relates to a high sensitivity marker arouses and detects structure.
Background
In vitro diagnostic products, also known as "IVD", and also known as "in vitro diagnostic products", are a technique in which specific biomarkers are labeled (for example, antibodies to be detected are labeled with enzyme/ferritin/colloidal gold), and then detected by a device. The specific biomarker generally refers to a certain characteristic biochemical index in a common physiological or pathological or therapeutic process for objective determination and evaluation, the progress of the biological process in which the organism is currently located can be known through determination of the specific biomarker, and a disease-specific biomarker is checked, so that the specific biomarker can help identification, early diagnosis and prevention of diseases and monitoring in the therapeutic process. However, the existing detection equipment has the defects of complex structure and low detection precision.
SUMMERY OF THE UTILITY MODEL
In order to overcome at least one of the above-mentioned prior art shortcomings, the utility model provides a high sensitivity marker arouses and detects structure.
According to one aspect of the present invention, there is provided a high-sensitivity marker excitation and detection structure, comprising a light source, a reflective lens and an image acquisition device; the reflecting lens is provided with an excitation light reflecting film so that the reflecting lens can reflect marker excitation light rays contained in light rays emitted by the light source, and the reflecting lens can allow light rays, except the marker excitation light rays, in the light rays emitted by the light source to penetrate through; the reflecting lens is arranged on a light emitting path of the light source, so that when the light source emits light, the reflecting lens reflects exciting light of the marker to an object to be detected; the image acquisition device is arranged in the direction extending in the direction opposite to the light path direction of the marker exciting light, so that after the marker exciting light irradiates the object to be detected, the marker response light reflected by the object to be detected can be acquired by the image acquisition device after penetrating through the reflecting lens.
When in use, the object to be detected is arranged on the reflection light path of the reflection lens; then, the light source is started, the light emitted by the light source comprises marker excitation light, the reflecting lens reflects the marker excitation light to the object to be detected, and the light emitted by the light source except the marker excitation light penetrates through the reflecting lens, so that noise and light interference generated by reflecting stray light to the object to be detected is avoided; then wait to detect the thing and send marker response light, marker response light propagates along the reverse extending direction of the light path direction of marker exciting light, because the wavelength of marker response light is different with marker exciting light, behind the marker response light sees through the reflection lens, is caught by image acquisition device and acquires, and image acquisition device obtains the image that contains marker response light spot quantity and distribution position to supply computer or manual work to carry out high accuracy ground analysis, the utility model discloses still have simple structure, characteristics with low costs.
In some embodiments, further comprising a first convex lens; the first convex lens is arranged on the light path of the marker excitation light.
Like this, the arrangement back that a plurality of incident angles that the speculum reflected are different passes through first convex lens to the mark exciting light that waits to detect on the thing is shone to the form of parallel light, the mark response light that waits to detect the thing reflection also jets out along the opposite direction with mark exciting light with the mode of parallel light then, and then the arrangement back focus of rethread first convex lens, the loss of mark response light has been avoided, be favorable to image acquisition device to obtain more mark response light, thereby can obtain more clear accurate image.
In some embodiments, a second convex lens is further included; the second convex lens is disposed in a direction extending in a direction opposite to the optical path direction of the marker excitation light.
Therefore, after the marker response light is focused after being sorted by the first convex lens, the marker response light in each direction can be defocused and dispersed along with the extension of the marker response light in each direction in the direction far away from the focus, and then after being sorted by the second convex lens, the marker response light in each direction is irradiated onto the image acquisition device in the form of parallel light, so that the image acquisition device can acquire clearer and more accurate images.
In some embodiments, the device further comprises a variegated color filter, and the variegated color filter is arranged on the light incident side of the image acquisition device.
Therefore, the variegated filter filters variegated colors in marker response light, and eliminates the interference of the variegated colors, so that the image acquisition device can acquire clearer and more accurate images.
In some embodiments, the light-emitting path of the light source is inclined with respect to the normal direction of the reflective mirror, so that the marker excitation light and the light emitted by the light source except for the marker excitation light do not overlap.
In some embodiments, the light emitting path of the light source is inclined at an angle of 45 ° to the normal direction of the reflector.
In some embodiments, the optical imaging system further comprises a support structure, wherein the first convex lens, the reflector plate, the variegated filter, the second convex lens and the image acquisition device are sequentially arranged on the support structure; the light source is arranged on the supporting structure and arranged beside the reflector.
In some embodiments, a lens barrel space is provided on the support structure, the first convex lens, the reflective lens, the variegated filter and the second convex lens are sequentially disposed in the lens barrel space, and the image capture device is hermetically disposed on the tail end of the lens barrel space; the light emitting part of the light source is arranged on the inner wall of the lens cone space.
Like this, through setting gradually first convex lens, the reflection mirror piece, variegated light filter and second convex lens in the lens cone space, and image acquisition device sets up on the tail end in lens cone space inclosed, the propagation of marker excitation light and marker response light can not receive the interference of external veiling glare so, be favorable to image acquisition device to obtain more clear accurate image, simultaneously, through setting up the light-out portion of light source on the inner wall in lens cone space, avoided setting up open window in bearing structure's lens cone space and supplied the light that the light source sent to get into, the seal in lens cone space has been strengthened.
In some embodiments, the system further comprises an image analysis computer, and the image acquisition device is electrically and electrically connected with the image analysis computer.
Therefore, the image acquisition device can be analyzed by the image analysis computer to obtain the image containing the number and the distribution position of the marker response light spots, and the working efficiency is improved.
In some embodiments, the mirror plate is a dichroic mirror.
Drawings
FIG. 1 is a schematic structural view of a high sensitivity marker excitation and detection structure according to an embodiment of the present invention;
FIG. 2 is a schematic view of the high sensitivity label excitation and detection structure of FIG. 1 disposed behind a support structure;
FIG. 3 is a schematic view of the perspective view of FIG. 2;
FIG. 4 is a cross-sectional view of FIG. 2;
FIG. 5 is a schematic diagram of the first convex lens and the second convex lens of FIG. 4 respectively aligning the marker excitation light and the marker response light;
FIG. 6 is a schematic representation of the high sensitivity label excitation and detection structure of FIG. 2 in cooperation with a 96-well plate.
Wherein the reference numerals have the following meanings:
1. a light source; 2. a mirror plate; 3. an image acquisition device; 4. an object to be detected; 5. a first convex lens; 6. a second convex lens; 7. a variegated color filter; 8. a support structure; 81. a barrel space; 9. 96-well plates.
Detailed Description
For better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Referring to fig. 1-5, the present invention provides a high-sensitivity marker exciting and detecting structure, which includes a light source 1, a reflector 2 and an image capturing device 3; the reflecting lens 2 is provided with an excitation light reflecting film, so that the reflecting lens 2 can reflect marker excitation light rays contained in the light rays emitted by the light source 1, and the reflecting lens 2 can allow light rays, except the marker excitation light rays, emitted by the light source 1 to penetrate through; the reflecting lens 2 is arranged on a light-emitting path of the light source 1, so that when the light source 1 emits light, the reflecting lens 2 reflects exciting light of the marker to the object to be detected 4; the image obtaining device 3 is disposed in a direction extending in a direction opposite to the light path direction of the marker excitation light, so that after the marker excitation light irradiates the object 4 to be detected, the marker response light reflected by the object 4 to be detected can be obtained by the image obtaining device 3 after passing through the reflecting lens 2. In detail, in this embodiment, the marker excitation light is red laser with a wavelength of 980nm, the reflection mirror 2 is provided with an excitation light reflection film as an enhanced reflection film, the thickness of the excitation light reflection film is 980nm/(2n), where n is the refractive index of the reflection mirror 2, so that the reflection mirror 2 only reflects the red laser, and other light emitted by the light source 1 can pass through the reflection mirror 2 and is not reflected to the object 4 to be detected. In the present embodiment, after the label exciting light is irradiated onto the object to be detected 4, the label response light reflected by the object to be detected 4 is green light having a wavelength of 532 nm. In other embodiments, the specific type of label exciting light can also be suitably adjusted according to the specific type of the object 4 to be detected and its corresponding label. In the present embodiment, the image pickup device 3 is a CCD. In other embodiments, AMPPD-ALP (adamantane-alkaline phosphatase) may also be used as the detection target, and cTnI (troponin) may be used as the marker.
When in use, an object 4 to be detected (for example, an antibody to be detected is marked by enzyme, ferritin and colloidal gold to obtain a sample) is arranged on a reflection light path of the reflector 2; then, the light source 1 is started, the light emitted by the light source 1 contains the label exciting light, the reflecting lens 2 reflects the label exciting light to the object 4 to be detected, and the light emitted by the light source 1 except the label exciting light penetrates through the reflecting lens 2, so that the noise and light interference generated by reflecting stray light to the object 4 to be detected is avoided; then wait to detect thing 4 and send marker response light, marker response light propagates along the reverse extending direction of the light path direction of marker exciting light, because the wavelength of marker response light is different with the marker exciting light, behind marker response light sees through reflection lens 2, is caught by image acquisition device 3 and acquires, and image acquisition device 3 obtains the image that contains marker response light spot quantity and distribution position to supply computer or manual work to carry out high accuracy ground analysis, the utility model discloses still have simple structure, characteristics with low costs.
As shown in fig. 1, 4 and 5, in the present embodiment, the present invention further includes a first convex lens 5; the first convex lens 5 is disposed on the optical path of the marker excitation light. Like this, the arrangement back that a plurality of incident angles that the speculum piece 2 reflects are different is through first convex lens 5 to treat the arrangement back, shine on treating the thing 4 with the form of parallel light, the thing 4 that treats that the reflected marker response light of thing also jets out along the opposite direction with the marker excitation light with the mode of parallel light then, and then focus behind the arrangement of first convex lens 5 again, the loss of marker response light has been avoided, be favorable to image acquisition device 3 to obtain more marker response light, thereby can obtain more clear accurate image.
As shown in fig. 1, 4 and 5, further, in the present embodiment, a second convex lens 6 is further included; the second convex lens 6 is disposed in the direction extending in the opposite direction to the optical path direction of the marker excitation light. In this way, after the marker response light is focused after being sorted by the first convex lens 5, the marker response light in each direction can be dispersed out of focus along with the extension of the marker response light in each direction towards the direction far away from the focus, and then after being sorted by the second convex lens 6, the marker response light in each direction is irradiated onto the image acquisition device 3 in the form of parallel light, so that the image acquisition device 3 can acquire a clearer and more accurate image.
In the present embodiment, a variegated color filter 7 is further included, and the variegated color filter 7 is provided on the light incident side of the image pickup device 3. In detail, in the present embodiment, the variegated color filter 7 is a blue color filter. In this way, the variegated filter 7 filters the variegated colors in the marker response light, and eliminates the interference of the variegated colors, thereby enabling the image obtaining device 3 to obtain a clearer and more accurate image.
In the present embodiment, the light emitting path of the light source 1 is inclined with respect to the normal direction of the reflective mirror 2, so that the label exciting light and the light emitted from the light source 1 except the label exciting light do not overlap.
In the present embodiment, the light emitting path of the light source 1 is inclined at an angle of 45 ° with respect to the normal direction of the reflector 2.
In the embodiment, the device further comprises a supporting structure 8, and the first convex lens 5, the reflector 2, the variegated filter 7, the second convex lens 6 and the image acquisition device 3 are sequentially arranged on the supporting structure 8; the light source 1 is arranged on the support structure 8 and the light source 1 is arranged beside the mirror plate 2. Thus, the utility model has the characteristics of compact structure.
As shown in fig. 2 to 4, in the present embodiment, a barrel space 81 is provided on the supporting structure 8, the first convex lens 5, the reflective lens 2, the variegated filter 7 and the second convex lens 6 are sequentially disposed in the barrel space 81, and the image capturing device 3 is hermetically disposed at the tail end of the barrel space 81; the light emitting portion of the light source 1 is disposed on the inner wall of the barrel space 81. Like this, through setting gradually first convex lens 5, mirror 2, variegated light filter 7 and second convex lens 6 in lens cone space 81, and image acquisition device 3 sets up on the tail end of lens cone space 81 airtightly, the propagation of marker excitation light and marker response light can not receive the interference of external veiling glare so, be favorable to image acquisition device 3 to obtain clearer and more accurate image, simultaneously, through setting up the portion of appearing of light source 1 on the inner wall of lens cone space 81, avoided setting up open window in lens cone space 81 of bearing structure 8 and supplied the light that light source 1 sent to get into, the leakproofness of lens cone space 81 has been strengthened.
In the present embodiment, an image analysis computer is further included, and the image acquisition device 3 is electrically and signally connected to the image analysis computer. Thus, the image acquisition device 3 can be analyzed by the image analysis computer to obtain the image containing the number and the distribution position of the marker response light spots, and the working efficiency is improved.
In the present embodiment, the mirror 2 is a dichroic mirror.
As shown in fig. 6, in the present embodiment, the object 4 to be detected is contained in a 96-well plate 9 made of a transparent optical material.
The technical means disclosed by the scheme of the present invention is not limited to the technical means disclosed by the above embodiments, but also includes the technical scheme formed by the arbitrary combination of the above technical features. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications are also considered as the protection scope of the present invention.
Claims (10)
1. High sensitivity marker excitation and detection structure characterized in that: the device comprises a light source, a reflector and an image acquisition device;
the reflecting lens is provided with an excitation light reflecting film so that the reflecting lens can reflect marker excitation light rays contained in light rays emitted by the light source, and the reflecting lens can allow light rays emitted by the light source except the marker excitation light rays to penetrate;
the reflecting lens is arranged on a light emitting path of the light source, so that when the light source emits light, the reflecting lens reflects the exciting light of the marker to an object to be detected;
the image acquisition device is arranged in the direction extending in the direction opposite to the light path direction of the marker exciting light, so that after the marker exciting light irradiates the object to be detected, the marker response light reflected by the object to be detected can be acquired by the image acquisition device after penetrating through the reflection lens.
2. The high-sensitivity label excitation and detection structure according to claim 1, further comprising a first convex lens;
the first convex lens is arranged on a light path of the marker excitation light.
3. The high-sensitivity label excitation and detection structure according to claim 2, further comprising a second convex lens;
the second convex lens is arranged in the direction extending in the opposite direction to the light path direction of the marker excitation light.
4. The high-sensitivity marker excitation and detection structure according to claim 3, further comprising a variegated color filter disposed on the light-incident side of said image capture device.
5. The high-sensitivity marker exciting and detecting structure according to claim 1, wherein the light emitting path of the light source is inclined with respect to the normal direction of the reflecting lens, so that the marker exciting light and the light emitted from the light source except for the marker exciting light do not overlap optically.
6. The high-sensitivity marker excitation and detection structure according to claim 5, wherein the light exit path of the light source is inclined at an angle of 45 ° to the normal direction of the mirror plate.
7. The high-sensitivity marker excitation and detection structure according to claim 4, further comprising a support structure on which the first convex lens, the mirror plate, the variegated color filter, the second convex lens, and the image capture device are sequentially disposed;
the light source is arranged on the supporting structure, and the light source is arranged beside the reflecting lens.
8. The high-sensitivity marker exciting and detecting structure according to claim 7, wherein a barrel space is provided on the supporting structure, the first convex lens, the reflector, the variegated filter and the second convex lens are sequentially disposed in the barrel space, and the image capturing device is hermetically disposed at a rear end of the barrel space;
the light emitting portion of the light source is disposed on an inner wall of the barrel space.
9. The high-sensitivity marker excitation and detection structure according to any one of claims 1 to 8, further comprising an image analysis computer, wherein the image acquisition device is in electrical signal connection with the image analysis computer.
10. The high-sensitivity label excitation and detection structure according to claim 1, wherein said reflective mirror is a dichroic mirror.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202120651626.4U CN214794465U (en) | 2021-03-30 | 2021-03-30 | High-sensitivity marker excitation and detection structure |
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| CN202120651626.4U CN214794465U (en) | 2021-03-30 | 2021-03-30 | High-sensitivity marker excitation and detection structure |
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| CN214794465U true CN214794465U (en) | 2021-11-19 |
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