CN214937508U - Anti-pollution device for real-time fluorescence PCR - Google Patents

Abstract

The utility model relates to an anti-pollution device for real-time fluorescence PCR. The test tube test device comprises a fixed support, a first movable support, a plunger pump, a second movable support, a motor, a motion module, a sleeve, a suction head, a first test tube, a second test tube, a third test tube and a fourth test tube, wherein the second movable support is arranged on the fixed support and is in sliding connection with a second sliding support along the Z-axis direction; the first movable support is arranged on the fixed support through the movement module and is positioned below the second movable support; first test tube, second test tube, third test tube and fourth test tube are placed on first movable support. Triple sealing is realized, pollution is effectively prevented, full-automatic operation is realized, and the efficiency is high; through the cooperation of closing cap and suction head shirk board, when preventing to pollute, also improved the security performance.

Description

Anti-pollution device for real-time fluorescence PCR

Technical Field

The utility model relates to a PCR technical field, more specifically relates to an anti-pollution device for real-time fluorescence PCR.

Background

The Polymerase Chain Reaction (PCR) technique is a method for rapid amplification of specific target nucleic acid fragments in vitro, and generally comprises three basic reaction steps of denaturation-annealing-extension, and amplification of target nucleic acids is realized through cycles of different temperatures.

The real-time fluorescence PCR technology is a nucleic acid/gene detection technology developed on the basis of the PCR technology, and is a method for monitoring the whole PCR process by adding a fluorescence labeling probe or a fluorescent dye into a PCR reaction system and utilizing the real-time detection of a fluorescence signal and finally carrying out qualitative or quantitative analysis on the nucleic acid or the gene through a specific fluorescence signal/a standard curve/an internal standard.

The real-time fluorescent PCR instrument has the biggest characteristics of larger amplification capacity, extremely high sensitivity and complex operation, so that pollution often occurs in the operation process, even a very small amount of pollution can cause false positive, and false negative can occur by slight error. For a long time, how to take effective measures to prevent pollution to the maximum extent is an important design requirement of the real-time fluorescence PCR instrument.

Chinese patent CN111197004A discloses a full-automatic real-time fluorescence quantitative PCR workstation, which realizes the full-automatic operation of the PCR process, but the device is easy to cause pollution in the operation process, and causes the problems of inaccurate measurement and the like.

SUMMERY OF THE UTILITY MODEL

The utility model discloses an overcome at least one defect among the above-mentioned prior art, provide an anti-pollution device for real-time fluorescence PCR, realized carrying out multiple sealing to the test tube of depositing the sample, effectively prevented the pollution of sample.

In order to solve the technical problem, the utility model discloses a technical scheme is: an anti-pollution device for real-time fluorescence PCR comprises a fixed support, a first movable support for storing a test tube, a plunger pump, a second movable support for fixing a sleeve and a suction head, a motor for driving the second movable support to move, a motion module for driving the first movable support to move on a horizontal plane, a sleeve, a suction head, a first test tube for storing a sample, a second test tube for storing a suction ball for ball sealing, a third test tube for storing liquid for liquid sealing and a fourth test tube for storing the suction head, wherein the second movable support is arranged on the fixed support and is in sliding connection with the second sliding support along the Z-axis direction; the first movable support is arranged on the fixed support through the movement module and is positioned below the second movable support; first test tube, second test tube, third test tube and fourth test tube equal vertical setting and demountable installation on first movable support. In the utility model, the first movable support is driven by the motion module to move left and right or back and forth on the horizontal plane so as to move the first test tube, the second test tube, the third test tube and the fourth test tube which are placed on the first movable support to the position right below the sleeve, so that the actions of sucking the liquid by the sucking head and sucking the sucking ball by the sleeve are realized, and the liquid and the sucking ball are moved to the first test tube; the second movable support is positioned right above the first movable support and can move up and down along the Z-axis direction under the driving of a motor so as to suck liquid from a test tube or drip liquid into the test tube when descending; when the anti-pollution device provided by the utility model is used, the suction head is placed in the fourth test tube, the first movable support is moved to enable the fourth test tube to be positioned under the sleeve, the motor drives the second movable support to descend, so that one end of the bottom of the sleeve presses the suction head to penetrate into the hole of the suction head, and the suction head is sleeved on the sleeve; then, repeatedly moving the first movable support and the second movable support to move the oil stored in the third test tube into the first test tube so as to realize the first resealing of the sample; then, the suction head is detached into a fourth test tube, the suction ball stored in the second test tube is moved into the first test tube through the sleeve to realize second resealing of the sample, and in addition, a cover can be arranged on the outer cover of the first test tube according to requirements to realize third resealing; multiple sealing, effectively prevented the pollution of sample, and full automatic operation, work efficiency is high.

In one embodiment, the second movable support comprises an elongated sleeve frame which is transversely arranged and is in sliding connection with the fixed support along the Z-axis direction, an elongated suction head detaching plate which is transversely arranged and is in sliding connection with the fixed support along the Z-axis direction and is positioned right below the sleeve frame, a plurality of first mounting through holes are arranged at intervals along the length direction of the sleeve frame, and a plurality of second mounting through holes which are in one-to-one correspondence with the first mounting through holes are arranged at intervals along the length direction of the suction head detaching plate; one end of the sleeve is fixed in the first mounting through hole, and the other end of the sleeve penetrates through the second mounting through hole and is connected with the suction head. The sleeve frame is used for fixing the sleeve, and the suction head detaching plate is used for ejecting the suction head through the suction head detaching plate when the suction head is detached; in addition, the tip removing plate can be used for pressing the sealing cover so that the sealing cover is only attached to the first test tube.

In one embodiment, a first annular boss is arranged in the second mounting through hole, and a second annular boss used for abutting against the first annular boss is arranged at one end of the suction head connected with the sleeve. When the suction head needs to be detached, the second motor drives the suction head detaching plate to move downwards, and the first annular boss abuts against the second annular boss on the suction head, so that the suction head is ejected, and the suction head is automatically detached.

In one embodiment, the depth values set by the first annular bosses in the second mounting through holes are at least two groups. The depth of the first annular bosses in the through holes is divided into at least two depth values, so that the suction head can be detached in batches, and the suction head is more labor-saving and safer.

In one embodiment, the motor comprises a first motor and a second motor; an output shaft of the first motor is connected with the sleeve frame and used for driving the sleeve frame to move up and down along the Z-axis direction; and an output shaft of the second motor is connected with the suction head detaching plate and is used for driving the suction head detaching plate to move up and down along the Z-axis direction.

In one embodiment, the test tube further comprises a cover for covering the nozzle of the first test tube, and the cover is detachably connected with the first test tube. After liquid seal and ball seal are completed, the sealing cover is covered to realize third seal, the sealing cover is tightly pressed through the suction head detaching plate, the cover can be prevented from being exploded in the heating process, and the safety performance is improved while pollution is effectively prevented.

In one embodiment, a sealing ring is arranged at the nozzle of the first test tube; one end of the first test tube, which is close to the tube opening, is provided with a ball sealing position for clamping the suction ball. The sealing ring is used for realizing the sealing connection between the sealing cover and the tube opening of the test tube; the ball block position is used for blocking the suction ball.

In one embodiment, a plurality of placing grooves are arranged on the first movable support at intervals, the placing grooves are distributed in multiple rows and are mutually parallel, and the spacing distance of the placing grooves in each row corresponds to the spacing distance of the first mounting through holes one by one; the first test tube, the second test tube, the third test tube and the fourth test tube are respectively placed in the placing grooves. The placing grooves are arranged in multiple rows, and test tubes with the same function are placed in the same row, so that the simultaneous operation is facilitated, and the efficiency is improved; the spacing distance between each two adjacent discharge slots is in one-to-one correspondence with the spacing distance between the sleeves, so that a plurality of sleeves or suction heads can be operated simultaneously, and the working efficiency is improved.

In one embodiment, the motion module comprises an X-axis moving system and a Y-axis moving system, and the first movable support is respectively connected with the X-axis moving system and the Y-axis moving system; each discharge slot of the first movable support is arranged along the X-axis direction, and the sleeve frame and the suction head detaching plate are also arranged along the X-axis direction.

In one embodiment, the test tube further comprises a third motor, a through hole is formed in the bottom of the second test tube, a thimble penetrates through the through hole, one end of the thimble is used for propping against the suction ball, and the other end of the thimble is connected with an output shaft of the third motor. The suction ball is ejected out through the ejector pin, so that the sleeve can conveniently suck the suction ball.

Compared with the prior art, the beneficial effects are: the utility model provides an anti-pollution device for real-time fluorescence PCR can realize oil blanket, ball seal, cap seal, triple seal, has effectively prevented the PCR test tube from being polluted, and triple seal full-automatic operation, and is efficient; by arranging a plurality of sleeves, suckers and test tubes corresponding to the sleeves, a plurality of samples can be operated in sequence, and the efficiency is high; through the cooperation of closing cap and suction head shirk board, when preventing to pollute, also improved the security performance.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic structural view of the sleeve and the second movable support of the present invention.

Fig. 3 is a schematic structural view of the first movable support of the present invention.

Fig. 4 is a schematic view of the first test tube structure of the present invention.

Figure 5 is a schematic view of the connection between the suction head and the sleeve according to the present invention.

Description of the drawings: 1. fixing a bracket; 2. a first movable support; 21. a placement groove; 3. a plunger pump; 4. a second movable support; 41. a sleeve frame; 42. a suction head detaching plate; 5. a motor; 51. a first motor; 52. a second motor; 6. a motion module; 7. a sleeve; 8. a suction head; 81. a second annular boss; 9. a first test tube; 91. the ball seals screens.

Detailed Description

The drawings are for illustrative purposes only and are not to be construed as limiting the invention; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the invention.

As shown in fig. 1 to 3, an anti-contamination device for real-time fluorescence PCR comprises a fixed bracket 1, a first movable bracket 2 for storing test tubes, a plunger pump 3, a second movable bracket 4 for fixing a sleeve 7 and a suction head 8, a motor 5 for driving the second movable bracket 4 to move, a motion module 6 for driving the first movable bracket 2 to move in a horizontal plane, a sleeve 7, a suction head 8, a first test tube 9 for storing samples, a second test tube for storing suction balls for ball seals, a third test tube for storing liquid for liquid seals, and a fourth test tube for storing the suction head 8, wherein the second movable bracket 4 is mounted on the fixed bracket 1 and is slidably connected with the second sliding bracket along a Z-axis direction, the sleeve 7 is vertically arranged and detachably mounted on the second movable bracket 4, one end of the sleeve 7 is connected with the plunger pump 3 through a pipeline, the other end is detachably connected with the suction head 8; the first movable support 2 is arranged on the fixed support 1 through the motion module 6 and is positioned below the second movable support 4; first test tube 9, second test tube, third test tube and the equal vertical setting of fourth test tube and demountable installation are on first movable support 2. In the utility model, the first movable support 2 is driven by the motion module 6 to move left and right or up and down on the horizontal plane, so as to move the first test tube 9, the second test tube, the third test tube and the fourth test tube which are placed on the first movable support 2 to the position right below the sleeve 7, and realize the actions of sucking the liquid by the sucking head 8, sucking the ball by the sleeve 7 and the like, thereby moving the liquid and the sucking ball to the first test tube 9; the second movable support 4 is positioned right above the first movable support 2, and the second movable support 4 can move up and down along the Z-axis direction under the driving of a motor 5 so as to suck liquid from a test tube or drip liquid into the test tube when descending; when the anti-pollution device provided by the utility model is used, the suction head 8 is placed in the fourth test tube, the first movable support 2 is moved to enable the fourth test tube to be positioned under the sleeve 7, the motor 5 drives the second movable support 4 to descend, and one end of the bottom of the sleeve 7 presses the suction head 8 downwards to penetrate into the hole of the suction head 8, so that the suction head 8 is sleeved on the sleeve 7; then, the first movable support 2 and the second movable support 4 are repeatedly moved to move the oil stored in the third test tube into the first test tube 9, so that the first resealing of the sample is realized; then, the suction head 8 is detached into a fourth test tube, the suction ball stored in the second test tube is moved into the first test tube 9 through the sleeve 7, so that second resealing of the sample is realized, and in addition, a cover can be covered on the outer cover of the first test tube 9 according to requirements, so that third resealing is realized; multiple sealing, effectively prevented the pollution of sample, and full automatic operation, work efficiency is high.

In some embodiments, as shown in fig. 2, the second movable support 4 includes an elongated sleeve frame 41 transversely disposed and slidably connected to the fixed support 1 along the Z-axis direction, an elongated tip removing plate 42 transversely disposed and slidably connected to the fixed support 1 along the Z-axis direction and located directly below the sleeve frame 41, a plurality of first mounting through holes are provided at intervals along the length direction of the sleeve frame 41, and a plurality of second mounting through holes corresponding to the first mounting through holes are provided at intervals along the length direction of the tip removing plate 42; one end of the sleeve 7 is fixed in the first mounting through hole, and the other end of the sleeve penetrates through the second mounting through hole and is connected with the suction head 8. The sleeve frame 41 is used for fixing the sleeve 7, and the suction head detaching plate 42 is used for ejecting the suction head 8 through the suction head detaching plate 42 when the suction head 8 is detached; alternatively, the tip release plate 42 may be used to press the closure so that it only engages the first test tube 9.

In some embodiments, a sliding groove is formed on the fixed bracket 1, and sliding blocks are arranged on the sleeve frame 41 and the suction head detachment plate 42, and are in sliding connection with the sliding groove through the sliding blocks, so that a limiting effect can be realized, and the motor 5 is prevented from shaking when the sleeve frame 41 and the suction head detachment plate 42 are driven to move up and down; the stability during movement is improved.

In one embodiment, as shown in fig. 5, a first annular boss is provided in the second mounting through hole, and a second annular boss 81 for abutting against the first annular boss is provided at the end of the suction head 8 connected to the sleeve 7. When the suction head 8 needs to be detached, the second motor 52 drives the suction head detaching plate 42 to move downwards, and the first annular boss abuts against the second annular boss 81 on the suction head 8, so that the suction head 8 is detached, and the suction head 8 is automatically detached.

In another embodiment, the depth values provided by the first annular bosses in the second plurality of mounting through holes are at least two sets. The depth of the first annular bosses in the through holes is divided into at least two depth values, so that the suction head 8 can be detached in batches, and the labor is saved and the safety is improved.

In one embodiment, 16 first mounting through holes are formed in the sleeve frame 41, 16 second mounting through holes are formed in the suction head detaching plate 42, 16 sleeves 7 are mounted on the sleeve frame 41, and each sleeve 7 is correspondingly provided with a suction head 8; each row of the same first movable support 2 is provided with 16 placing grooves 21, at least four discharging grooves 21 are arranged, and the placing grooves correspond to the first test tube 9, the second test tube, the third test tube and the fourth test tube respectively; thus, 16 groups of samples can be operated at one time during operation, and the efficiency is high; in addition, the depth value that first annular boss set up is equipped with 3 groups, and the depth value that first annular boss set up in every group has 5 second installation through-holes at least is the same, like this when taking off suction head 8, can divide into three groups and shirk, shirks 5 suction heads 8 at every turn, compares and shirks 16 suction heads together more laborsaving, safety once more.

In one embodiment, as shown in fig. 1 and 2, the motor 5 includes a first motor 51 and a second motor 52; an output shaft of the first motor 51 is connected with the sleeve frame 41 and used for driving the sleeve frame 41 to move up and down along the Z-axis direction; an output shaft of the second motor 52 is connected to the tip removing plate 42 for driving the tip removing plate 42 to move up and down in the Z-axis direction.

In some embodiments, a cap is further included for covering the mouth of the first test tube 9, the cap being removably connected to the first test tube 9. After liquid seal and ball seal are finished, the sealing cover is covered to realize third seal, the sealing cover is tightly pressed through the suction head detaching plate 42, the cover can be prevented from being exploded in the heating process, pollution is effectively prevented, and meanwhile, the safety performance is improved.

In one embodiment, a sealing ring is arranged at the nozzle of the first test tube 9; a ball sealing position 91 for clamping the suction ball is arranged at one end of the first test tube 9 close to the tube opening. The sealing ring is used for realizing the sealing connection between the sealing cover and the tube opening of the test tube; the ball block position 91 is used for blocking the suction ball.

As shown in fig. 4, the first test tube 9 may be provided with a plurality of first test tubes 9 connected in a row, such as in the form of 6-tubes, so as to facilitate the movement and handling.

In some embodiments, as shown in fig. 1 and 3, a plurality of placing slots 21 are spaced on the first movable bracket 2, the placing slots 21 are distributed in multiple rows and parallel to each other, and the spacing distance of the placing slots 21 in each row corresponds to the spacing distance of the first mounting through holes; the first test tube 9, the second test tube, the third test tube, and the fourth test tube are placed in the placement grooves 21, respectively. The placing grooves 21 are arranged in multiple rows, and test tubes with the same function are placed in the same row, so that the simultaneous operation is facilitated, and the efficiency is improved; the spacing distance between each of the discharging slots 21 corresponds to the spacing distance between the sleeves 7 one by one, so that a plurality of sleeves 7 or suction heads 8 can be operated simultaneously, thereby improving the working efficiency.

In one embodiment, as shown in fig. 3, the motion module 6 includes an X-axis moving system and a Y-axis moving system, and the first movable support 2 is connected to the X-axis moving system and the Y-axis moving system respectively; each of the discharge slots 21 of the first movable holder 2 is arranged in the X-axis direction, and the socket holder 41 and the tip removing plate 42 are also arranged in the X-axis direction.

In another embodiment, the test tube further comprises a third motor, a through hole is formed in the bottom of the second test tube, a thimble penetrates through the through hole, one end of the thimble is used for propping against the suction ball, and the other end of the thimble is connected with an output shaft of the third motor. The suction ball is ejected out through the ejector pin, so that the sleeve 7 can conveniently suck the suction ball.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be considered as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. An anti-pollution device for real-time fluorescence PCR is characterized by comprising a fixed support (1), a first movable support (2) for storing test tubes, a plunger pump (3), a second movable support (4) for fixing a sleeve (7) and a suction head (8), a motor (5) for driving the second movable support (4) to move, a motion module (6) for driving the first movable support (2) to move on a horizontal plane, a sleeve (7), the suction head (8), a first test tube (9) for storing samples, a second test tube for storing suction balls for ball seals, a third test tube for storing liquid for liquid seals and a fourth test tube for storing the suction head (8), wherein the second movable support (4) is arranged on the fixed support (1) and is in sliding connection with the second movable support (4) along the Z-axis direction, the sleeve (7) is vertically arranged and detachably arranged on the second movable support (4), one end of the sleeve (7) is connected with the plunger pump (3) through a pipeline, and the other end of the sleeve is detachably connected with the suction head (8); the first movable support (2) is arranged on the fixed support (1) through a motion module (6); first test tube (9), second test tube, third test tube and fourth test tube equal vertical setting and demountable installation on first movable support (2).

2. The anti-contamination device for real-time fluorescent PCR according to claim 1, wherein the second movable support (4) comprises an elongated sleeve frame (41) which is transversely arranged and slidably connected with the fixed support (1) along the Z-axis direction, an elongated tip detachment plate (42) which is transversely arranged and slidably connected with the fixed support (1) along the Z-axis direction, a plurality of first mounting through holes are formed at intervals along the length direction of the sleeve frame (41), and a plurality of second mounting through holes which correspond to the first mounting through holes in position one-to-one are formed at intervals along the length direction of the tip detachment plate (42); one end of the sleeve (7) is fixed in the first mounting through hole, and the other end of the sleeve penetrates through the second mounting through hole and is connected with the suction head (8).

3. The anti-contamination device for real-time fluorescent PCR according to claim 2, wherein the second mounting through hole is provided with a first annular boss, and the end of the suction head (8) connected to the sleeve (7) is provided with a second annular boss (81) for abutting against the first annular boss.

4. The contamination prevention device for real-time fluorescent PCR according to claim 3, wherein the depth values provided to the first annular bosses in the second plurality of mounting through-holes are provided in at least two sets.

5. The antipollution device for real-time fluorescent PCR according to claim 3, characterized in that said motor (5) comprises a first motor (51) and a second motor (52); an output shaft of the first motor (51) is connected with the sleeve frame (41) and used for driving the sleeve frame (41) to move up and down along the Z-axis direction; and an output shaft of the second motor (52) is connected with the suction head detaching plate (42) and is used for driving the suction head detaching plate (42) to move up and down along the Z-axis direction.

6. The anti-contamination device for real-time fluorescent PCR according to claim 3, further comprising a cap for covering the nozzle of the first cuvette (9), the cap being detachably connected to the first cuvette (9).

7. The anti-contamination device for real-time fluorescent PCR according to claim 6, characterized in that a sealing ring is provided at the mouth of the first cuvette (9); one end of the first test tube (9) close to the tube opening is provided with a ball sealing and clamping position (91) for clamping the suction ball.

8. The contamination prevention device for real-time fluorescent PCR according to claim 7, wherein a plurality of placing grooves (21) are spaced on the first movable support (2), the plurality of placing grooves (21) are arranged in a plurality of rows and are parallel to each other, and the spacing distance of the plurality of placing grooves (21) in each row corresponds to the spacing distance of the plurality of first mounting through holes one by one; the first test tube (9), the second test tube, the third test tube and the fourth test tube are respectively placed in the placing grooves (21).

9. The anti-contamination device for real-time fluorescent PCR according to claim 8, wherein the motion module (6) comprises an X-axis moving system and a Y-axis moving system, and the first movable support (2) is connected with the X-axis moving system and the Y-axis moving system respectively; each discharge slot (21) of the first movable support (2) is arranged along the X-axis direction, and the sleeve frame (41) and the suction head detaching plate (42) are also arranged along the X-axis direction.

10. The anti-contamination device for real-time fluorescence PCR according to any one of claims 1 to 9, further comprising a third motor, wherein the bottom of the second test tube is provided with a through hole, a thimble penetrates through the through hole, one end of the thimble is used for propping against the suction ball, and the other end of the thimble is connected with an output shaft of the third motor.

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