CN214937484U - Bioreactor - Google Patents
Bioreactor Download PDFInfo
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- CN214937484U CN214937484U CN202120523657.1U CN202120523657U CN214937484U CN 214937484 U CN214937484 U CN 214937484U CN 202120523657 U CN202120523657 U CN 202120523657U CN 214937484 U CN214937484 U CN 214937484U
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Abstract
The utility model belongs to the technical field of it is biological, specifically disclose a bioreactor, including reaction vessel, new liquid storage container, waste liquid storage container and infusion set, waste liquid storage container and new liquid storage container all communicate with reaction vessel through infusion set and pipe fitting, and infusion set can be simultaneously to input liquid in the reaction vessel and export liquid from reaction vessel. The scheme can realize the updating and the replacement of the culture solutionThe bacteria can be cultured continuously for a long time, the culture solution is prevented from being replaced again when being cultured every time, the operation is more convenient, in addition, the new culture solution can be supplied into the bioreactor under the uniform speed control, meanwhile, the bacteria solution can be discharged at the uniform speed, the aim of controlling the growth rate in the bioreactor to be constant is fulfilled, and the pH sensor, the dissolved oxygen sensor and the CO are utilized2The sensors and the temperature sensors can realize pH and O adjustment by means of the signal processing terminal2、CO2And the real-time monitoring of the temperature meets more experimental requirements.
Description
Technical Field
The utility model belongs to the technical field of it is biological, especially, relate to a bioreactor.
Background
At present, the bacteria are mainly cultured in a shaking table in a large quantity, but the existence of the bacteria cannot monitor the growth metabolic indexes (such as PH value and CO) of the bacteria in real time2Concentration, O2Concentration, etc.). Moreover, the quantitative culture solution can only be put in one time during each culture, and the renewal and replacement of the culture solution cannot be controlled, so that the number of bacteria cannot be effectively controlled.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a bioreactor realizes the renewal and the change of culture solution, can be long-term, incessant cultivates the bacterium, has avoided cultivateing at every turn and all need whole change culture solution again, and it is more convenient to operate.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
a bioreactor comprises a reaction container, a new liquid storage container, a waste liquid storage container and an infusion device, wherein the reaction container is used for containing culture solution, the waste liquid storage container and the new liquid storage container are communicated with the reaction container through the infusion device and a pipe fitting, and the infusion device can input liquid into the reaction container and output liquid from the reaction container.
The principle of the scheme is as follows: when the device is used, the infusion device is started, the culture solution for planting bacteria, which is contained in the bacteria culture solution storage container, is introduced into the reaction container through the pipe fitting by the infusion device, and the infusion is stopped after the culture solution in the reaction container reaches a certain amount. Then, the culture solution in the reaction vessel is stirred to make the culture solution in the reaction vessel reach a better environmental condition. Then, the transfusion device is started again, the flow speed of input and output is set, the culture solution is added into the reaction container from the new solution storage container at a constant speed, the bacterial culture solution is discharged into the waste solution storage container from the reaction container at a constant speed, the output speed and the input speed can be set to be the same or different according to requirements, the purpose of controlling the growth speed and the temperature in the bioreactor is achieved, long-term and uninterrupted culture of bacteria is achieved, the situation that the culture solution needs to be replaced integrally every time of culture is avoided, and the operation is more convenient.
Preferably, the reactor also comprises a constant temperature water bath box, the reaction container is of a double-layer structure, the inner layer is a reaction chamber for containing culture solution, a water circulation water bath chamber is arranged between the inner layer and the outer layer, a water outlet of the constant temperature water bath box is connected with the bottom of the water bath chamber through a pipe fitting, and a water inlet of the constant temperature water bath box is connected with the upper part of the water bath chamber through a pipe fitting. The internal environment of the reaction chamber is kept in a constant temperature state through the constant temperature water bath box and the water bath room, and the culture effect is better.
Preferably, the top cover of the reaction vessel is provided with a cover body, the cover body is provided with a plurality of openings, and each opening is respectively connected with a pH sensor and a dissolved oxygen sensor、CO2A sensor and a temperature sensor, wherein each sensor extends into the reaction vessel. Can real-timely measure the pH, dissolved oxygen and CO of the culture solution2And the temperature and the like are monitored to ensure that the environmental characteristics in the reaction vessel are not different.
Preferably, the reactor also comprises a stirring mechanism, wherein the stirring mechanism comprises a motor and a stirring paddle driven by the motor, the stirring paddle extends into the reaction chamber, and the motor is positioned above the reaction container. The nutrient solution is stirred by the electric stirring mechanism, and the effect of stirring and culturing bacteria is better.
Preferably, the device also comprises a processing terminal, wherein the processing terminal is connected with a pH sensor, a dissolved oxygen sensor and CO2The sensor and the temperature sensor are in signal connection, and the processing terminal is used for connecting the pH sensor, the dissolved oxygen sensor and the CO2And signals output by the sensor and the temperature sensor are transmitted to the cloud receiver. The treatment terminal can adjust pH, dissolved oxygen and CO2And the characteristic values such as temperature and the like are transmitted to a cloud receiver, and then the characteristic values of the culture solution are monitored by a computer in real time, so that the environmental characteristics in the reaction container are ensured to be free from variation.
Preferably, the infusion device can simultaneously input and output liquid into and from the reaction vessel. With this arrangement, the amount of the liquid to be fed into and the amount of the liquid to be fed out from the reaction vessel can be always maintained in a certain equilibrium state, that is, the culture medium in the reaction vessel can be maintained in a stable state, thereby ensuring a good culture environment.
Preferably, the infusion means is a bi-directional peristaltic pump capable of delivering liquid to and from the reaction vessel.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a schematic view of a reactor having a water bath structure in the example;
FIG. 3 is a schematic view of a reactor having various sensors in the example;
FIG. 4 is a schematic view of a reactor having a stirring structure in the example.
Reference numerals in the drawings of the specification include: reaction vessel 1, reactionA chamber 101, a water bath chamber 102, a transfusion device 3, a new liquid storage container 4, a waste liquid storage container 5, a water outlet pipe 6, a water inlet pipe 7, a liquid outlet pipe 8, a constant temperature water bath tank 9, a motor 10, a stirring paddle 11, a pH sensor 12, a temperature sensor 13, a liquid inlet pipe 14, CO2Sensor 15, dissolved oxygen sensor 16.
Detailed Description
The following is further detailed by way of specific embodiments:
the embodiment is basically as shown in the attached figure 1: a bioreactor comprises a reaction container 1, a new liquid storage container 4, a waste liquid storage container 5 and an infusion device 3, wherein the reaction container 1 is used for containing culture solution, the waste liquid storage container 5 and the new liquid storage container 4 are communicated with the reaction container 1 through the infusion device 3 and a pipe fitting, and the infusion device 3 can input liquid into the reaction container 1 and output liquid from the reaction container 1.
Wherein, be the culture room of bacterium in the reaction vessel 1, new liquid storage container 4 is used for storing brand-new culture solution, mainly for reaction vessel 1 supply culture solution, and waste liquid storage container 5 is used for storing the old and useless culture solution of following reaction vessel 1 exhaust, and in this embodiment, new liquid storage container 4 and waste liquid storage container 5 are blue mouthful bottles, and reaction vessel 1 is the glass container.
When the device is used, the infusion device 3 is started, the culture solution for planting bacteria contained in the new solution storage container 4 is introduced into the reaction container 1 through the pipe fitting by the infusion device 3, and the infusion is stopped after the culture solution in the reaction container 1 reaches a certain amount. Then, the culture solution in the reaction vessel 1 is stirred, the transfusion device 3 is started, the flow speed of input and output is set, the culture solution is dripped into the reaction vessel 1 from the new solution storage vessel 4 at a constant speed, and the bacterial culture solution is discharged into the waste solution storage vessel 5 from the reaction vessel 1 at a constant speed, so that the culture solution is updated and replaced, the bacteria can be cultured continuously for a long time, the situation that the culture solution needs to be replaced integrally every time of culture is avoided, and the operation is more convenient.
Specifically, the infusion device 3 is a multi-channel peristaltic pump capable of bidirectional output, in this embodiment, the peristaltic pump is a basic peristaltic pump, the model of the driver is L100-1E, and the model of the pump head is DG-2 (10 rollers). The peristaltic pump is provided with two channels and four connecting ports, namely a water inlet A1, a water outlet B1, a water inlet A2 and a water outlet B2, the bottom of a reaction container 1 is communicated with a liquid outlet pipe 8, the top of the reaction container 1 is communicated with a liquid inlet pipe 14, the water inlet A1 is communicated with the liquid outlet pipe 8 at the bottom of the reaction container 1 through a hose, and the water outlet B1 is connected with a waste liquid storage container 5 through a hose; the inlet water A2 is connected with a new liquid storage container 4 through a hose, and the outlet water B2 is connected with an inlet pipe 14 at the top of the reaction container 1. During operation, start the peristaltic pump, can make the culture solution of reaction vessel 1 bottom carry to waste liquid storage container 5 through income water A1, play water B1 in, when the discharge waste liquid, the peristaltic pump can carry the culture solution in the new liquid storage container 4 to reaction vessel 1 upper portion through income water A2, play water B2, and can set for peristaltic pump output rate as required and input rate the same or different, reach best cultivation effect.
In some embodiments, the infusion device 3 may also be two or more ordinary one-way infusion pumps, and when there are two infusion pumps, one of the infusion pumps may be used to deliver the culture solution in the bottom of the reaction vessel 1 to the waste liquid storage vessel 5, and the other infusion pump may be used to deliver the culture solution in the new liquid storage vessel 4 to the upper part of the reaction vessel 1, so as to achieve the effect of simultaneous two-way output.
Referring to fig. 2, the reactor further comprises a water bath structure, which comprises a constant temperature water bath tank 9, wherein the constant temperature water bath tank 9 is a DC-0506 water bath tank of the Shanghai balance instrument. And reaction vessel 1 is double glazing structure, and the inlayer is the reaction chamber 101 of splendid attire culture solution, is the bath room 102 between the inside and outside layer, and reaction chamber 101 is used for loading culture solution, and bath room 102 is used for the circulating water that flows, and in this embodiment, reaction vessel 1 chooses the double glazing reation kettle of autumn wool technology for use, and wherein, reaction vessel 1's volume is 3L, and the capacity of bath room 102 is 0.6L. The upper part of the left side of the water bath room 102 is connected with a water outlet pipe 6, the lower part of the right side is connected with a water inlet pipe 7, wherein the water outlet pipe 6 is connected with a water inlet of the constant temperature water bath box 9 through a heat-resistant pipe, and the water inlet pipe 7 is connected with a water outlet of the constant temperature water bath box 9 through a heat-resistant pipe. When the reactor is operated, the circulating water output from the constant temperature water bath tank 9 enters from the bottom of the water bath chamber 102 and is discharged from the upper part of the water bath chamber 102, and the internal environment of the reaction chamber 101 is maintained in a constant temperature state during the culture process.
Referring to fig. 3, the top of the reaction vessel 1 is further covered with a glass cover, the specific shape of the cover is not limited, and the cover in this embodiment is an arc-shaped structure, and the cover may be integrally formed on the reaction vessel 1, or may be covered on the reaction vessel 1 in a detachable connection manner such as buckling, screwing, and the like.
The cover body is provided with a plurality of openings, and each opening is respectively connected with a pH sensor 12, a dissolved oxygen sensor 16 and CO2A sensor 15 and a temperature sensor 13, each extending into the reaction chamber 101. Wherein, the dissolved oxygen sensor 16 is AMT-D0300 intelligent dissolved oxygen sensor, the pH sensor 12 is AMT-PH300 intelligent pH value sensor, CO2AMT-CO2300 intelligent CO is selected as sensor2A sensor.
In order to facilitate the signal processing, the reactor is externally connected with processing terminals (not shown in the figure), specifically, the processing terminals are connected with the pH sensor 12, the dissolved oxygen sensor 16, and CO2The sensor 15 and the temperature sensor 13 are connected by communication lines and the like, and the processing terminal is used for connecting the pH sensor 12, the dissolved oxygen sensor 16 and CO2The signals output by the sensor 15 and the temperature sensor 13 are transmitted to the cloud receiver, and the processing terminal in this embodiment is a computer, so that the characteristic values of the culture solution can be monitored in real time through the computer.
Referring to fig. 4, the reaction container 1 is further provided with a stirring mechanism, specifically, the stirring mechanism includes a motor 10 and a stirring paddle 11 driven by the motor 10, wherein the motor 10 is fixedly mounted on a fixing frame (not shown in the fixing frame) located above the reaction container 1, the stirring paddle 11 extends into the reaction container 1, and the motor 10 is started to drive the stirring paddle 11 to uniformly stir the culture solution in the reaction container 1, in this embodiment, the stirring speed is 0 to 420 r/min.
When the water bath device is used specifically, the constant temperature water bath tank 9 is started, the water temperature is set to be 37 ℃, circulating water output by the constant temperature water bath tank 9 enters from the bottom of the water bath chamber 102 and is discharged from the upper part of the water bath chamber 102, and water bath circulation is started, so that the internal environment of the reaction chamber 101 is kept in a constant temperature state.
Then, the peristaltic pump is started to pump the culture solution in the new solution storage container 4 into the reaction chamber 101, and the transfusion is stopped when the culture solution in the reaction chamber 101 reaches a certain amount. Then, the motor 10 is started, and the motor 10 drives the stirring paddle 11 to stir the culture solution in the reaction chamber 101. And then, the peristaltic pump is started, the functions of infusion and liquid drainage are started simultaneously, the flow speed of the infusion and the liquid drainage is set, the culture solution is added into the reaction chamber 101 from the new liquid storage container 4 at a constant speed, and the bacteria culture solution is discharged into the waste liquid storage container 5 from the reaction chamber 101 at a constant speed, so that the culture solution is updated and replaced, the bacteria can be cultured continuously for a long time, the situation that the culture solution needs to be replaced integrally every time of culture is avoided, and the operation is more convenient. In the working process, the pH, the dissolved oxygen and the CO of the culture solution are monitored in real time by a computer2The concentration and temperature, etc., ensure the culture environment of the reaction chamber 101.
The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the teachings of the present invention without undue experimentation. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.
Claims (8)
1. A bioreactor, characterized by: including reaction vessel (1), new liquid storage container (4), waste liquid storage container (5) and infusion set (3), reaction vessel (1) is used for splendid attire culture solution, and waste liquid storage container (5) and new liquid storage container (4) are all through infusion set (3) and pipe fitting and reaction vessel (1) intercommunication, and infusion set (3) can be to inputing liquid in reaction vessel (1) and export liquid from reaction vessel (1).
2. A bioreactor according to claim 1, wherein: the reaction container is of a double-layer structure, the inner layer is a reaction chamber (101) for containing culture solution, a water circulation water bath chamber (102) is arranged between the inner layer and the outer layer, a water outlet of the constant-temperature water bath tank (9) is connected with the bottom of the water bath chamber (102) through a pipe fitting, and a water inlet of the constant-temperature water bath tank (9) is connected with the upper part of the water bath chamber (102) through a pipe fitting.
3. A bioreactor according to claim 1 or 2, wherein: the top of the reaction container (1) is covered with a cover body, a plurality of openings are arranged on the cover body, and each opening is respectively connected with a pH sensor (12), a dissolved oxygen sensor (16) and CO2A sensor (15) and a temperature sensor (13), each of which extends into the reaction vessel (1).
4. A bioreactor according to claim 1 or 2, wherein: the reactor also comprises a stirring mechanism, wherein the stirring mechanism comprises a motor (10) and a stirring paddle (11) driven by the motor (10), the stirring paddle (11) extends into the reaction container (1), and the motor (10) is positioned above the reaction container (1).
5. A bioreactor according to claim 3, wherein: the reactor also comprises a stirring mechanism, wherein the stirring mechanism comprises a motor (10) and a stirring paddle (11) driven by the motor (10), the stirring paddle (11) extends into the reaction chamber (101), and the motor (10) is positioned above the reaction container (1).
6. A bioreactor according to claim 5, wherein: also comprises a processing terminal, the processing terminal is connected with the pH sensor (12), the dissolved oxygen sensor (16) and the CO2The sensor (15) and the temperature sensor (13) are in signal connection, and the processing terminal is used for connecting the pH sensor (12), the dissolved oxygen sensor (16) and the CO2Signals output by the sensor (15) and the temperature sensor (13) are transmitted to the cloud receiver.
7. A bioreactor according to claim 1, 2 or 5, wherein: the liquid feeding device (3) can simultaneously feed liquid into the reaction vessel (1) and discharge liquid from the reaction vessel (1).
8. A bioreactor according to claim 1, 2 or 5, wherein: the liquid-feeding device (3) is a bidirectional peristaltic pump which can feed liquid into the reaction vessel (1) and discharge liquid from the reaction vessel (1).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120512019X | 2021-03-11 | ||
| CN202120512019 | 2021-03-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214937484U true CN214937484U (en) | 2021-11-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120523657.1U Active CN214937484U (en) | 2021-03-11 | 2021-03-12 | Bioreactor |
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|---|---|
| CN (1) | CN214937484U (en) |
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2021
- 2021-03-12 CN CN202120523657.1U patent/CN214937484U/en active Active
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