CN214881509U - Multifunctional auxiliary device for fluorescence quantitative PCR detection - Google Patents
Multifunctional auxiliary device for fluorescence quantitative PCR detection Download PDFInfo
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- CN214881509U CN214881509U CN202120809358.4U CN202120809358U CN214881509U CN 214881509 U CN214881509 U CN 214881509U CN 202120809358 U CN202120809358 U CN 202120809358U CN 214881509 U CN214881509 U CN 214881509U
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Abstract
The utility model belongs to the technical field of fluorescence quantitative PCR detects with supplementary detection, a have multi-functional fluorescence quantitative PCR detects with auxiliary device is related to, wherein, comprising a base plate, be provided with the box on the bottom plate, first spout and second spout have been seted up to inner wall one side of box, the one end fixedly connected with motor of first spout. Its beneficial effect is, this multi-functional fluorescence quantitative PCR detects uses auxiliary device, through the syringe, first hydraulic pressure electric putter, the setting of second hydraulic pressure electric putter and third hydraulic pressure electric putter etc, can inhale the syringe with the reagent in the reagent cup in, and can remove the syringe directly over the detecting tube, conveniently make the sample in the detecting tube and reagent take place the reaction, under the combined action of motor, threaded rod and screw section of thick bamboo etc., can take the detecting tube to remove the one side of box, thereby can place the detecting tube on PCR detection device's the test-tube rack.
Description
Technical Field
The utility model belongs to the technical field of fluorescence quantitative PCR detects with supplementary detection, concretely relates to fluorescence quantitative PCR detects with auxiliary device with it is multi-functional.
Background
The Polymerase Chain Reaction (PCR) is a nucleic acid amplification technology simulating the natural DNA replication process in vitro, the principle is similar to the replication of natural DNA, the PCR is a method for selectively synthesizing specific DNA by in vitro enzymatic reaction, and the reaction of real-time fluorescence quantitative PCR is carried out by adding fluorescent dye and forming a cycle of high-temperature thermal denaturation, low-temperature renaturation and suitable-temperature extension, so that DNA fragments can be rapidly amplified. In the whole circulation process, the product is marked and monitored, the fluorescence detection device is utilized to obtain real-time fluorescence, the multifunctional real-time fluorescence quantitative PCR system is an analytical instrument used in the fields of basic medicine, clinical medicine, preventive medicine and public health,
in order to solve the above problems, a multifunctional auxiliary device for fluorescence quantitative PCR detection is proposed.
SUMMERY OF THE UTILITY MODEL
To solve the problems set forth in the background art described above. The utility model provides a multi-functional fluorescence quantitative PCR detects uses auxiliary device, it has solved the easy contaminated technical problem of sample.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an auxiliary device for PCR detection of fluorescence ration with it is multi-functional, includes the bottom plate, be provided with the box on the bottom plate, first spout and second spout have been seted up to inner wall one side of box, the one end fixedly connected with motor of first spout, fixedly connected with threaded rod on the output shaft of motor, threaded connection has a screw thread section of thick bamboo on the threaded rod, the first backup pad of fixed surface of a screw thread section of thick bamboo, be provided with fixture in the first backup pad, the last joint of fixture has the test tube.
The utility model discloses a syringe, including box, inner wall one side fixed connection fixed plate, the third spout has been seted up on the fixed plate, the first hydraulic pressure electric putter of one side fixedly connected with of third spout, sliding connection has the sliding block in the third spout, the one end of first hydraulic pressure electric putter and the top fixed connection of sliding block, one side fixedly connected with second hydraulic pressure electric putter of sliding block, the other end fixedly connected with second backup pad of second hydraulic pressure electric putter, fixedly connected with third hydraulic pressure electric putter in the second backup pad, third hydraulic pressure electric putter's other end fixedly connected with cardboard, be provided with the joint spare in the second backup pad, the joint has same syringe on cardboard and the joint spare.
As a further aspect of the present invention: the first sliding groove and the second sliding groove are communicated with each other, a limiting block is connected in the second sliding groove in a sliding mode, and the bottom of the limiting block is fixedly connected with the outer surface of the threaded cylinder.
As a further aspect of the present invention: the bottom of the inner wall of the box body is fixedly connected with a placing table, and a reagent cup is arranged on the placing table.
As a further aspect of the present invention: the box body is characterized in that a fourth sliding groove is formed in the bottom plate, a sliding plate is connected in the fourth sliding groove in a sliding mode, a fourth hydraulic electric push rod is fixedly connected to one side of the fourth sliding groove, the other end of the fourth hydraulic electric push rod is fixedly connected with one side of the sliding plate, and the top of the sliding plate is fixedly connected with the bottom of the box body.
As a further aspect of the present invention: sliding chutes are formed in two sides of the inner wall of the box body, and baffles are connected in the sliding chutes in a sliding mode.
As a further aspect of the present invention: one side swing joint of box has the chamber door, one side of box is provided with the controller, the controller passes through wire electric connection with motor, fixture, first hydraulic pressure electric putter, second hydraulic pressure electric putter and third hydraulic pressure electric putter respectively.
Compared with the prior art, the beneficial effects of the utility model are that:
1. this auxiliary device for fluorescence quantitative PCR detects with it is multi-functional, through the syringe, first hydraulic pressure electric putter, the setting of second hydraulic pressure electric putter and third hydraulic pressure electric putter etc, can inhale the syringe with the reagent in the reagent cup, and can remove the syringe directly over the detecting tube, conveniently make the sample in the detecting tube and reagent take place to react, under the combined action of motor, threaded rod and screw thread section of thick bamboo etc, can take the detecting tube to remove the one side of box, thereby can place the detecting tube on PCR detection device's the test-tube rack.
2. This auxiliary device for fluorescence quantitative PCR detects with it is multi-functional, through fixture's setting, conveniently carry out the centre gripping and loose putting to detecting the test tube, under the combined action of fourth hydraulic pressure electric putter and sliding plate etc. can remove whole box towards PCR detection device's direction.
3. This multi-functional fluorescence quantitative PCR auxiliary device for detection through the setting of controller, can control motor, first hydraulic pressure electric putter, second hydraulic pressure electric putter, third hydraulic pressure electric putter, fourth hydraulic pressure electric putter and fixture's operating condition.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural view of a front view section of the present invention;
FIG. 2 is a front view of the present invention;
fig. 3 is a schematic three-dimensional structure diagram of the pilot tube rack of the present invention;
in the figure: 1. a box body; 2. a first chute; 3. a second chute; 4. a threaded rod; 5. a limiting block; 6. a motor; 7. a second support plate; 8. a second hydraulic electric push rod; 9. a first hydraulic electric push rod; 10. a slider; 11. a third chute; 12. a fixing plate; 13. a reagent cup; 14. a placing table; 15. a fourth hydraulic electric push rod; 16. a sliding plate; 17. a fourth chute; 18. a base plate; 19. a sliding groove; 20. a first support plate; 21. a clamping mechanism; 22. a detection tube; 23. a third hydraulic electric push rod; 24. clamping a plate; 25. an injector; 26. a clamping piece; 27. a baffle plate; 28. a box door; 29. and a controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Examples
Referring to fig. 1-3, the present invention provides the following technical solutions: a multifunctional auxiliary device for fluorescence quantitative PCR detection comprises a bottom plate 18, a box body 1 is arranged on the bottom plate 18, a fourth sliding groove 17 is formed in the bottom plate 18, a sliding plate 16 is connected in the fourth sliding groove 17 in a sliding mode, a fourth hydraulic electric push rod 15 is fixedly connected to one side of the fourth sliding groove 17, the other end of the fourth hydraulic electric push rod 15 is fixedly connected to one side of the sliding plate 16, the top of the sliding plate 16 is fixedly connected to the bottom of the box body 1, the whole box body 1 can move towards the direction of the PCR detection device under the combined action of the fourth hydraulic electric push rod 15, the sliding plate 16 and the like, a first sliding groove 2 and a second sliding groove 3 are formed in one side of the inner wall of the box body 1, a motor 6 is fixedly connected to one end of the first sliding groove 2, a threaded rod 4 is fixedly connected to an output shaft of the motor 6, a threaded barrel is connected to the threaded rod 4 in a threaded mode, and the first sliding groove 2 and the second sliding groove 3 are communicated with each other, a limiting block 5 is connected in the second chute 3 in a sliding way, the bottom of the limiting block 5 is fixedly connected with the outer surface of the threaded cylinder, the outer surface of the threaded cylinder is fixedly connected with a first supporting plate 20, the threaded cylinder has a certain length, the detection tube 22 can be driven to enter the PCR detection device, the connection mode of the thread cylinder and the sliding block, the position of the first support plate 20 can be selected according to actual requirements, the clamping mechanism 21 is arranged on the first support plate 20, the clamping mechanism 21 can automatically clamp the detection tube 22, and the detection tube is a conventional tube, the details are omitted, the holding mechanism 21 is provided to facilitate the holding and releasing of the test tube, the holding mechanism 21 is clamped with the test tube 22, under the combined action of the motor 6, the threaded rod 4, the threaded cylinder and the like, the detection tube 22 can be moved to one side of the box body 1, so that the detection tube 22 can be placed on a test tube rack of the PCR detection device.
A fixed plate 12 is fixedly connected to one side of the inner wall of the box body 1, a third chute 11 is formed in the fixed plate 12, a first hydraulic electric push rod 9 is fixedly connected to one side of the third chute 11, a sliding block 10 is connected in the third chute 11 in a sliding manner, one end of the first hydraulic electric push rod 9 is fixedly connected to the top of the sliding block 10, a second hydraulic electric push rod 8 is fixedly connected to one side of the sliding block 10, a second supporting plate 7 is fixedly connected to the other end of the second hydraulic electric push rod 8, a third hydraulic electric push rod 23 is fixedly connected to the second supporting plate 7, a clamping plate 24 is fixedly connected to the other end of the third hydraulic electric push rod 23, through the arrangement of an injector 25, the first hydraulic electric push rod 9, the second hydraulic electric push rod 8, the third hydraulic electric push rod 23 and the like, the reagent in the reagent cup 13 can be sucked into the injector 25, and the injector 25 can be moved to the position right above the detection tube 22, the convenient sample and the reagent that make in the test tube 22 react, are provided with joint spare 26 in the second backup pad 7, and the joint has same syringe 25 on cardboard 24 and the joint spare 26, and the platform 14 is placed to the inner wall bottom fixedly connected with of box 1, is provided with reagent cup 13 on placing the platform 14.
Specifically, sliding grooves 19 have all been seted up to the inner wall both sides of box 1, and sliding connection has baffle 27 in two sliding grooves 19.
Specifically, a box door 28 is movably connected to one side of the box body 1, a controller 29 is arranged on one side of the box body 1, the controller 29 can be a computer or other conventional known device which plays a role in control, the controller 29 is respectively electrically connected with the motor 6, the clamping mechanism 21, the first hydraulic electric push rod 9, the second hydraulic electric push rod 8 and the third hydraulic electric push rod 23 through conducting wires, and through the arrangement of the controller 29, the working states of the motor 6, the first hydraulic electric push rod 9, the second hydraulic electric push rod 8, the third hydraulic electric push rod 23, the fourth hydraulic electric push rod 15 and the clamping mechanism 21 can be controlled.
The utility model discloses a theory of operation does:
s1, when in use, the bottom plate 18 is placed or installed at the entrance of the PCR detection device, then the box door 28 is opened, a new syringe 25 is clamped on the clamping plate 24 and the clamping piece 26, then the reagent cup 13 is placed on the placing table 14, and then the box door 28 is closed;
s2, the controller 29 controls the first hydraulic electric push rod 9 to descend with the slide block 10, so that the bottom end of the injector 25 is positioned in the reagent cup 13, the third hydraulic electric push rod 23 is shortened, so that the injector 25 sucks the reagent in the reagent cup 13, then the controller 29 controls the first hydraulic electric push rod 9 to be shortened, then the second hydraulic electric push rod 8 extends to move towards the detection tube 22, and stops when the reagent reaches the position right above the detection tube 22, next, the controller 29 controls the first hydraulic electric push rod 9 to extend, so that the bottom end of the injector 25 is positioned in the detection tube 22, then the controller 29 controls the third hydraulic electric push rod 23 to extend, so that the reagent in the injector 25 can be extruded into the detection tube 22, and then the injector 25 returns to the initial position;
s3, then, the controller 29 controls the fourth hydraulic electric push rod 15 to drive the sliding plate 16 to slide, thereby the box body 1 can be integrally moved towards the direction of the PCR detection device and stops after reaching a proper position, then, the baffle plate 27 is opened, the controller 29 controls the motor 6 to rotate, under the limit of the limit block 5 and the second sliding groove 3, the threaded cylinder is enabled to translate on the threaded rod 4, when the detection tube 22 is positioned right above the test tube rack of the PCR detection device, the controller 29 controls the clamping mechanism 21 not to clamp the detection tube 22 any more, the detection tube 22 is enabled to be clamped on the test tube rack, the whole process has no manual participation, and the pollution to the sample is avoided.
While embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that modifications, alterations, substitutions and variations may be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (6)
1. The utility model provides an auxiliary device for fluorescence quantitative PCR detects with multi-functional, includes bottom plate (18), its characterized in that: the detection box is characterized in that a box body (1) is arranged on the bottom plate (18), a first sliding groove (2) and a second sliding groove (3) are formed in one side of the inner wall of the box body (1), a motor (6) is fixedly connected to one end of the first sliding groove (2), a threaded rod (4) is fixedly connected to an output shaft of the motor (6), a threaded cylinder is connected to the threaded rod (4) in a threaded mode, a first supporting plate (20) is fixedly connected to the outer surface of the threaded cylinder, a clamping mechanism (21) is arranged on the first supporting plate (20), and a detection tube (22) is clamped on the clamping mechanism (21);
a fixed plate (12) is fixedly connected to one side of the inner wall of the box body (1), a third sliding groove (11) is formed in the fixed plate (12), a first hydraulic electric push rod (9) is fixedly connected to one side of the third sliding groove (11), a sliding block (10) is connected to the third sliding groove (11) in a sliding mode, one end of the first hydraulic electric push rod (9) is fixedly connected to the top of the sliding block (10), a second hydraulic electric push rod (8) is fixedly connected to one side of the sliding block (10), a second supporting plate (7) is fixedly connected to the other end of the second hydraulic electric push rod (8), a third hydraulic electric push rod (23) is fixedly connected to the second supporting plate (7), a clamping plate (24) is fixedly connected to the other end of the third hydraulic electric push rod (23), and a clamping piece (26) is arranged on the second supporting plate (7), the clamping plate (24) and the clamping piece (26) are clamped with the same injector (25).
2. The multifunctional fluorescent quantitative PCR detection auxiliary device according to claim 1, wherein: first spout (2) and second spout (3) communicate each other, sliding connection has stopper (5) in second spout (3), the bottom of stopper (5) and the fixed surface of a screw thread section of thick bamboo are connected.
3. The multifunctional fluorescent quantitative PCR detection auxiliary device according to claim 1, wherein: the bottom of the inner wall of the box body (1) is fixedly connected with a placing table (14), and a reagent cup (13) is arranged on the placing table (14).
4. The multifunctional fluorescent quantitative PCR detection auxiliary device according to claim 1, wherein: the box body is characterized in that a fourth sliding groove (17) is formed in the bottom plate (18), a sliding plate (16) is connected in the fourth sliding groove (17) in a sliding mode, a fourth hydraulic electric push rod (15) is fixedly connected to one side of the fourth sliding groove (17), the other end of the fourth hydraulic electric push rod (15) is fixedly connected to one side of the sliding plate (16), and the top of the sliding plate (16) is fixedly connected with the bottom of the box body (1).
5. The multifunctional fluorescent quantitative PCR detection auxiliary device according to claim 1, wherein: sliding grooves (19) are formed in two sides of the inner wall of the box body (1), and baffles (27) are connected in the sliding grooves (19) in a sliding mode.
6. The multifunctional fluorescent quantitative PCR detection auxiliary device according to claim 1, wherein: one side swing joint of box (1) has chamber door (28), one side of box (1) is provided with controller (29), controller (29) pass through wire electric connection with motor (6), fixture (21), first hydraulic pressure electric putter (9), second hydraulic pressure electric putter (8) and third hydraulic pressure electric putter (23) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120809358.4U CN214881509U (en) | 2021-04-20 | 2021-04-20 | Multifunctional auxiliary device for fluorescence quantitative PCR detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120809358.4U CN214881509U (en) | 2021-04-20 | 2021-04-20 | Multifunctional auxiliary device for fluorescence quantitative PCR detection |
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| CN214881509U true CN214881509U (en) | 2021-11-26 |
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| CN202120809358.4U Active CN214881509U (en) | 2021-04-20 | 2021-04-20 | Multifunctional auxiliary device for fluorescence quantitative PCR detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196528A (en) * | 2022-01-18 | 2022-03-18 | 奥谱天成（厦门）光电有限公司 | PCR detector based on it is multispectral |
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2021
- 2021-04-20 CN CN202120809358.4U patent/CN214881509U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196528A (en) * | 2022-01-18 | 2022-03-18 | 奥谱天成（厦门）光电有限公司 | PCR detector based on it is multispectral |
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