CN214781893U

CN214781893U - Temperature-controllable cell culture device

Info

Publication number: CN214781893U
Application number: CN202023282929.8U
Authority: CN
Inventors: 马东兰
Original assignee: Zhengzhou Yao Jiao Medical Instrument Ltd
Current assignee: Zhejiang Gaomei Biotechnology Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2021-11-19
Anticipated expiration: 2030-12-30

Abstract

The utility model discloses a controllable cell culture device of temperature, including the box, the top block of box is connected with the case lid, and the inner wall of bottom half is fixed to be equipped with heating device, and heating device is including heating chamber, heat pipe and copper guide, and the fixed bottom that sets up at the box inner wall in heating chamber, the fixed a plurality of heat pipes that are equipped with of bottom equidistance in heating chamber, and the middle part of every two heat pipes is all fixed to be equipped with the copper guide, and the top in heating chamber is fixed to be equipped with the backing plate, and the fixed household utensils device that is equipped with in top of backing plate, the beneficial effects of the utility model are that: the liquid storage bottle arranged in the clamping groove facilitates the operation of the device during cell culture; the heat transfer efficiency among the heat conduction pipes is higher through the copper guide sheets, and the cells are prevented from being damaged due to too low temperature; the internal condition of the device is conveniently observed through the observation window; the temperature of the cells cultured by the device can be observed at any time through a temperature controller; make liquid storage bottle stability higher through two stopper.

Description

Temperature-controllable cell culture device

Technical Field

The utility model relates to a culture apparatus, in particular to controllable cell culture device of temperature belongs to cell culture technical field.

Background

In the bioengineering technology, cell culture is an indispensable process, and cell culture generally puts into the blake bottle with the cell, and then puts into the incubator and advances, can take out the blake bottle from the incubator during cell culture and put under the microscope in order to observe the growth condition of cell, and the incubator under the normal condition is difficult to control the box temperature, and the too high temperature or the too low temperature all are unfavorable for the growth of cell in the blake box, and the too low temperature easily leads to the cell membrane to break, can cause cell death when serious.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a controllable cell culture device of temperature to the problem of the difficult control box temperature who provides among the solution above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a temperature-controllable cell culture device comprises a box body, wherein the top end of the box body is connected with a box cover in a clamping manner, the inner wall of the bottom of the box body is fixedly provided with a heating device, the heating device comprises a heating cavity, heat conducting pipes and copper conducting pieces, the heating cavity is fixedly arranged at the bottom of the inner wall of the box body, the bottom of the heating cavity is fixedly provided with a plurality of heat conducting pipes at equal intervals, every two heat conducting pipes are fixedly provided with copper conducting pieces at the middle parts, the top end of the heating cavity is fixedly provided with a backing plate, the top end of the backing plate is fixedly provided with a vessel device, the vessel device comprises a placing plate, a culture tank, a culture plate and a plurality of liquid containing tanks, the placing plate is fixedly arranged at the middle part of the top end of the backing plate, the culture tank is arranged at the middle part of the top end of the placing plate, the inner wall of the culture tank is connected with the culture plate in a clamping manner, and a plurality of the liquid containing tanks are arranged at the middle part of the culture plate, the fixed battery case that is equipped with in one side of box inner wall, the top of battery case articulates there is the battery cover, the inside of battery case is provided with the battery, the fixed fixture block that is equipped with in one side on backing plate top, the draw-in groove has been seted up at the middle part on fixture block top, the inner wall block of draw-in groove is connected with liquid storage bottle, the card hole has been seted up to the inner wall of case lid one side, the both sides on card hole top are all fixed and are equipped with the stopper.

As a preferred technical scheme of the utility model, the fixed temperature control appearance that is equipped with in one side of backing plate bottom inner wall, it is a plurality of the heat pipe all passes through temperature control appearance and battery electric connection.

As an optimized technical scheme of the utility model, it is a plurality of the bottom in flourishing cistern all is fixed and is equipped with the carrier film.

As an optimal technical scheme of the utility model, the width of width and draw-in groove between two stoppers all is the same with the width of liquid storage bottle.

As an optimized technical scheme of the utility model, the observation window is inlayed at the middle part of case lid.

As an optimized technical scheme of the utility model, the both sides on liquid storage bottle top all are equipped with the block rubber.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model relates to a temperature-controllable cell culture device, which is convenient to operate when the device is used for cell culture through a liquid storage bottle arranged in a clamping groove; the heat transfer efficiency among the heat conduction pipes is higher through the copper guide sheets, and the cells are prevented from being damaged due to too low temperature; the internal condition of the device can be conveniently observed by workers through the observation window; the temperature of the device for culturing cells can be adjusted at any time through a temperature controller; the cell culture is safer and the storage is more convenient through a plurality of bearing films; make liquid storage bottle place stability higher through two stopper.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic sectional structure of the present invention;

FIG. 3 is an enlarged schematic view of the structure of FIG. 1;

fig. 4 is an enlarged schematic structural diagram of fig. 2B according to the present invention.

In the figure: 1. a box body; 2. a box cover; 3. a heating device; 4. a base plate; 5. a vessel means; 6. a battery case; 7. a battery cover; 8. a storage battery; 9. a clamping block; 10. a card slot; 11. a liquid storage bottle; 12. a clamping hole; 13. a limiting block; 14. a temperature controller; 15. a carrier film; 16. an observation window; 31. a heating cavity; 32. a heat conducting pipe; 33. a copper lead; 51. placing the plate; 52. a culture tank; 53. culturing the plate; 54. a liquid containing tank.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a temperature-controllable cell culture apparatus, comprising a case 1, a case cover 2 connected to the top end of the case 1, a heating device 3 fixed to the inner wall of the bottom of the case 1, the heating device 3 including a heating chamber 31, heat pipes 32 and copper fins 33, the heating chamber 31 fixed to the bottom of the inner wall of the case 1, the bottom of the heating chamber 31 fixed to a plurality of heat pipes 32 at equal intervals, the copper fins 33 fixed to the middle of each two heat pipes 32, a backing plate 4 fixed to the top of the heating chamber 31, a vessel device 5 fixed to the top of the backing plate 4, the vessel device 5 including a placing plate 51, a culture tank 52, a culture plate 53 and a plurality of liquid containers 54, the placing plate 51 fixed to the middle of the top of the backing plate 4, the culture tank 52 formed in the middle of the top of the placing plate 51, the culture tank 52 connected to the culture plate 53, cultivate board 53's middle part and seted up a plurality of flourishing cistern 54, the fixed battery case 6 that is equipped with in one side of box 1 inner wall, the top of battery case 6 articulates there is battery cover 7, the inside of battery case 6 is provided with battery 8, the fixed fixture block 9 that is equipped with in one side on backing plate 4 top, draw-in groove 10 has been seted up at the middle part on fixture block 9 top, the inner wall block of draw-in groove 10 is connected with liquid storage bottle 11, card hole 12 has been seted up to the inner wall of case lid 2 one side, the both sides on card hole 12 top are all fixed and are equipped with stopper 13.

Preferably, the top of one side of the box body 1 is fixedly provided with a temperature controller 14, so that the temperature controller 14 can control the temperature of the cells during cell culture, and the cell membranes of the cultured cells are not easy to break; the bottom parts of the liquid containing tanks 54 are all fixedly provided with the bearing films 15, and the bearing films 15 can protect cells more effectively; the width between the two limiting blocks 13 and the width of the clamping groove 10 are the same as the width of the liquid storage bottle 11, so that the liquid storage bottle 11 is higher in stability when being placed; an observation window 16 is embedded in the middle of the box cover 2, so that workers can observe the cell culture condition in the box body 1 conveniently; the both sides on 11 tops of liquid storage bottle all are equipped with the rubber piece, and the frictional force when increasing to get and put makes 11 difficult drops when getting of liquid storage bottle.

When the temperature controllable cell culture device is used, firstly, the temperature range of the temperature controller 14 with the model REX-C100 is set at 36-40 ℃, when the temperature in the box body 1 reaches 40 ℃, the temperature controller 14 controls the plurality of heat pipes 32 to stop working, the backing plate 4 enables the heat transfer to be slow, so that the cells adapt to the temperature more easily, then the liquid storage bottle 11 in the clamping groove 10 is taken out and the cell culture solution and the cells are put in, then the box cover 2 is opened, the cell culture solution and the cells in the liquid storage bottle 11 are poured into the plurality of liquid containing grooves 54 in the culture plate 53 slowly, the culture plate 53 is arranged in the culture groove 52 at the top end of the placing plate 51, so that the heat is transferred to the liquid containing grooves 54 after decreasing progressively, the situation that the cells cannot adapt due to the too fast rise of the heat is prevented, then the bottom end of the liquid storage bottle 11 is placed in the clamping groove 10 formed at the top end of the clamping block 9 through the clamping hole 12, the liquid storage bottle 11 is stably placed through the clamping groove 10 and the two limiting blocks 13, when the temperature in the box body 1 is lower than 36 ℃, the temperature controller 14 controls the heat conduction pipes 32 to start working to heat the interior of the box body 1, and the battery box 6 is hinged to the battery cover 7 to enable the storage battery 8 in the battery box 6 to be convenient to replace.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a controllable cell culture device of temperature, includes box (1), its characterized in that, the top block of box (1) is connected with case lid (2), the fixed heating device (3) that is equipped with in inner wall of box (1) bottom, heating device (3) are including heating chamber (31), a plurality of heat pipe (32) and a plurality of copper guide (33), heating chamber (31) are fixed to be set up the bottom of box (1) inner wall, the fixed a plurality of heat pipe (32) that are equipped with in bottom equidistance of heating chamber (31), per two the middle part of heat pipe (32) is all fixed and is equipped with copper guide (33), the fixed backing plate (4) that is equipped with in top of heating chamber (31), the fixed household utensils device (5) that is equipped with in top of backing plate (4), household utensils device (5) are including placing board (51), culture groove (52), The novel box body is characterized by comprising a culture plate (53) and a plurality of liquid containing grooves (54), wherein the placing plate (51) is fixedly arranged in the middle of the top end of the base plate (4), the middle of the top end of the placing plate (51) is provided with the culture groove (52), the inner wall of the culture groove (52) is connected with the culture plate (53) in a clamping manner, the middle of the culture plate (53) is provided with the plurality of liquid containing grooves (54), one side of the inner wall of the box body (1) is fixedly provided with a battery box (6), the top end of the battery box (6) is hinged with a battery cover (7), the inside of the battery box (6) is provided with a storage battery (8), one side of the top end of the base plate (4) is fixedly provided with a clamping block (9), the middle of the top end of the clamping block (9) is provided with a clamping groove (10), the inner wall of the clamping groove (10) is connected with a liquid storage bottle (11), and the inner wall of one side of the box cover (2) is provided with a clamping hole (12), and limiting blocks (13) are fixedly arranged on two sides of the top end of the clamping hole (12).

2. A temperature-controllable cell culture apparatus according to claim 1, wherein: one side of the inner wall of the bottom of the backing plate (4) is fixedly provided with a temperature controller (14), and the heat conduction pipes (32) are electrically connected with the storage battery (8) through the temperature controller (14).

3. A temperature-controllable cell culture apparatus according to claim 1, wherein: the bottoms of the liquid containing tanks (54) are all fixedly provided with bearing films (15).

4. A temperature-controllable cell culture apparatus according to claim 1, wherein: the width between the two limiting blocks (13) and the width of the clamping groove (10) are the same as the width of the liquid storage bottle (11).

5. A temperature-controllable cell culture apparatus according to claim 1, wherein: an observation window (16) is embedded in the middle of the box cover (2).

6. A temperature-controllable cell culture apparatus according to claim 1, wherein: and rubber blocks are arranged on two sides of the top end of the liquid storage bottle (11).