CN214750352U - Quick bigeminy of many select allergen specificity IgE detects test-paper box - Google Patents
Quick bigeminy of many select allergen specificity IgE detects test-paper box Download PDFInfo
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- CN214750352U CN214750352U CN202120751105.6U CN202120751105U CN214750352U CN 214750352 U CN214750352 U CN 214750352U CN 202120751105 U CN202120751105 U CN 202120751105U CN 214750352 U CN214750352 U CN 214750352U
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Abstract
The utility model relates to a quick bigeminy test paper box of multiple choice allergen specificity IgE, including the shell, the shell forms a cavity, the cavity is equipped with a baffle and separates the cavity into two containing tanks of the same size, the one end of two containing tanks respectively is equipped with an opening, the top surface of containing tank is close to opening one end and is equipped with observation hole and dropping hole in proper order, the line of observation hole and dropping hole is parallel to the baffle; still include allergen detection test paper strip more than two kinds and, the test paper strip is including adhering to in proper order on the polyvinyl chloride bottom plate and between adjacent part superpose sample pad, colloidal gold combine pad, nitrocellulose membrane, absorbent paper, nitrocellulose membrane contains detection line and quality control line, the test paper strip can be followed the opening and inserted in the storage tank. The test paper box can detect two kinds of allergens simultaneously to can be according to the allergen that doctor's first visit and patient's demand independent assortment detected, realized the individuation and the diversification that the allergen detected.
Description
Technical Field
The utility model relates to an immunodetection technical field, concretely relates to quick bigeminy of multiple choice allergen specificity IgE detects test paper box.
Background
Clinical allergen-specific IgE antibodies are subjected to less in-vitro rapid qualitative detection and are not popularized yet, and the existing detection kits in the market comprise a colloidal gold-immunoblotting kit and a colloidal gold-immunochromatography kit. The colloidal gold-immunoblotting kit can detect mite-specific IgE antibodies. The colloidal gold-immunochromatography kit can detect various allergen-specific IgE antibodies. The detection method has short detection time and low cost.
The prior art has the following disadvantages:
1. can not be used for specific allergen immunotherapy before primary screening. Specific allergen immunotherapy, also known as desensitization therapy, is currently the only treatment for allergic causes. There are 3 kinds of desensitizing drugs sold in China, namely Changdi (dust mite drops), Andeda (house dust mite allergen preparation) and Allog (mite allergen injection), wherein Changdi is suitable for patients with dust mite allergy, Andeda is suitable for patients with house dust mite, and Allog is suitable for patients with dust mite and house dust mite allergy. Because the treatment modes, treatment costs and treatment time of various desensitization medicaments are different, the desensitization medicaments suitable for patients are provided for selection, so that the acceptance and compliance of the patients can be greatly improved, and the initial screening of house dust mite/dermatophagoides farinae allergens before desensitization treatment of the patients is very important. The existing allergen rapid detection kits (colloidal gold-immunoblotting kits and colloidal gold-immunochromatography kits) on the market at present can not be distinguished directly to judge whether the patients are allergic to the mites or which mites are allergic to the mites, and the kits can not judge whether the patients need desensitization treatment or which drugs can be desensitized, which influences the primary judgment of clinicians and can not give more detailed and professional clinical treatment suggestions to the patients.
2. In immunochromatographic assays, the flow of liquid has a significant influence on the immunochromatographic assay reaction. The presence of red blood cells may affect the flow process of the whole reaction system and affect the immune response. Common methods for obtaining serum or plasma by separating red blood cells from a blood sample include natural sedimentation, which requires a long waiting time, and centrifugation, which requires the use of a dedicated centrifugation device, and both of which have difficulties in meeting the demand for point-of-care (poct) tests. The colloidal gold-immunoblotting method kit and the colloidal gold-immunochromatography method kit which exist in the market require professionals to extract whole blood of patients, separate or naturally settle the whole blood under a centrifuge, then absorb serum to drip on test paper strips, and read results. The series of operations can only be detected by professional staff in a medical place, and a great deal of time and energy are consumed, so that the workload of medical staff in a hospital is increased, the time of doctors and patients is wasted, and real instant detection cannot be realized.
3. The colloidal gold-immunochromatography kit can detect IgE antibodies specific to various allergens, but the conventional allergen rapid detection test strip on the market has two detection lines (inhalation/ingestion allergens), each detection line contains 6 allergens, and if the result is positive, which allergen is allergic cannot be judged.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a use fast convenient, can qualitative detect allergen more than two kinds simultaneously to can free combination collocation detection object's quick bigeminy detection test paper box of many choice allergen specificity IgE.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a multi-choice allergen specificity IgE quick dual detection test paper box comprises a shell, wherein the shell forms a cavity, the cavity is provided with a baffle plate to divide the cavity into two containing grooves with the same size, one ends of the two containing grooves are respectively provided with an opening, one ends of the top surfaces of the containing grooves, which are close to the openings, are sequentially provided with an observation hole and a dropping hole, and the connecting line of the observation hole and the dropping hole is parallel to the baffle plate;
the test strip comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the absorbent paper are sequentially adhered to a polyvinyl chloride bottom plate and are partially overlapped between adjacent detection lines and quality control lines, and the test strip can be inserted into the accommodating groove from the opening;
after the test strip is inserted into the containing groove from the opening, the test strip sample pad corresponds to the dropping hole in the upper and lower directions, and the test strip nitrocellulose membrane corresponds to the observation hole in the upper and lower directions.
Furthermore, one end of the polyvinyl chloride bottom plate of the test strip, which is close to the absorbent paper, protrudes outwards to form a drawing tail end.
Further, the allergen detection test strip comprises a house dust mite test strip and a dust mite test strip.
Further, the allergen detection test strip comprises a house dust mite test strip, a cat hair test strip, a dog hair test strip and a wormwood test strip.
Further, the test strip comprises a sample pad coated with an anti-erythrocyte antibody.
Further, the test strip comprises a colloidal gold conjugate pad coated with anti-human IgE-secondary antibody labeled with colloidal gold and chicken IgY.
Furthermore, the house dust mite test strip comprises a detection line coated with house dust mite allergen and a quality control line coated with goat anti-chicken IgY.
Furthermore, the dust mite test strip comprises a detection line coated with dust mite allergen and a quality control line coated with goat anti-chicken IgY.
Furthermore, the cat hair test strip comprises a detection line coated with cat hair allergen and a quality control line coated with goat anti-chicken IgY, and the dog hair test strip comprises a detection line coated with dog hair allergen and a quality control line coated with goat anti-chicken IgY.
Further, the wormwood test strip comprises a detection line coated with wormwood allergen and a quality control line coated with goat anti-chicken IgY.
The utility model has the advantages that:
1. the test paper box of the utility model can simultaneously detect two allergens, and provides five common allergen detection test paper strips, which is convenient and rapid, and has wide applicability; the test paper strip can be extracted from the shell for replacement, can freely combine the detected allergen according to the needs of doctors for initial diagnosis and patients, and realizes the individuation and diversification of allergen detection.
2. Test paper strip is with low costs, easy and simple to handle and weak point consuming time can realize allergen specificity IgE's quick qualitative detection, has huge clinical application latent energy, and the testing result can be used to guide clinician's preliminary judgement.
The technical solution of the present invention will be described in detail with reference to the accompanying drawings.
Drawings
Fig. 1 is an external view of a housing according to the present invention;
fig. 2 is a top view of a housing profile according to the present invention;
fig. 3 is a schematic structural diagram of one of the test strips of the present invention.
Wherein: 1-shell, 2-baffle, 3-containing groove, 4-opening, 5-observation hole, 6-dropping hole, 7-polyvinyl chloride bottom plate, 8-sample pad, 9-colloidal gold combined pad, 10-nitrocellulose membrane, 11-absorbent paper, 12-detection line, 13-quality control line and 70-extraction tail end.
Detailed Description
In the description of the present invention, it is to be understood that the terms "radial", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Please refer to fig. 1 and fig. 2, which are an external view of the housing and a top view of the housing section, respectively.
A multi-choice allergen specificity IgE quick dual detection test paper box is characterized by comprising a shell 1, wherein the shell forms a cavity, the cavity is provided with a baffle 2 to divide the cavity into two containing grooves 3 with the same size, one ends of the two containing grooves 3 are respectively provided with an opening 4, one ends of the top surfaces of the containing grooves 3, which are close to the openings 4, are sequentially provided with an observation hole 5 and a dropping hole 6, and the connecting line of the observation hole 5 and the dropping hole 6 is parallel to the baffle 2;
still include two kinds and more than allergen detection test paper strips, please refer to fig. 3, it is a structural schematic diagram of one of them test paper strips of the utility model.
The test strip comprises a sample pad 8, a colloidal gold bonding pad 9, a nitrocellulose membrane 10 and absorbent paper 11 which are sequentially adhered to a polyvinyl chloride bottom plate 7 and are partially overlapped between adjacent sample pads, wherein the nitrocellulose membrane 10 comprises a detection line 12 and a quality control line 13, and the test strip can be inserted into the accommodating groove 3 from an opening;
after the test strip is inserted into the containing groove 3 from the opening, the test strip sample pad 8 corresponds to the dropping hole 6 up and down, and the test strip nitrocellulose membrane 10 corresponds to the observation hole 5 up and down.
Preferably, one end of the polyvinyl chloride bottom plate 7 of the test strip, which is close to the absorbent paper, protrudes outwards to form an extraction end 70, which is convenient for an operator to extract and place the test strip from the test paper box.
Preferably, the allergen detection test strip comprises a house dust mite test strip and a dust mite test strip, and the detection result can guide a clinician to preliminarily judge whether the dust mite immune preparation needs to be applied and which immune preparation is specifically used.
Preferably, the allergen detection test strip comprises a house dust mite test strip, a cat hair test strip, a dog hair test strip and a wormwood test strip, which are all common allergens and have wide applicability.
Preferably, the test strip includes the sample pad 8 that is wrapped with anti erythrocyte antibody, and anti erythrocyte antibody can be with the erythrocyte interception in the whole blood in the sample pad, realizes the erythrocyte separation in the whole blood sample, has saved the step and the time of erythrocyte separation in the testing process, also can satisfy the patient and detect by oneself simultaneously, and is more convenient.
Preferably, the strip comprises a colloidal gold conjugate pad 9 coated with a colloidal gold labeled anti-human IgE-secondary antibody and chicken IgY.
Preferably, the house dust mite test strip comprises a detection line 12 coated with house dust mite allergen and a quality control line 13 coated with goat anti-chicken IgY.
Preferably, the dust mite test strip comprises a detection line 12 coated with dust mite allergen and a quality control line 13 coated with goat anti-chicken IgY.
Preferably, the cat hair test strip comprises a detection line 12 coated with cat hair allergen and a quality control line 13 coated with goat anti-chicken IgY, and the dog hair test strip comprises a detection line 12 coated with dog hair allergen and a quality control line 13 coated with goat anti-chicken IgY.
Preferably, the wormwood test strip comprises a detection line 12 coated with wormwood allergen and a quality control line 13 coated with goat anti-chicken IgY.
The preparation method of the test strip of the utility model comprises the following steps:
(1) the glass fiber of the test strip sample pad is coated with anti-RBC antibody.
(2) Coating: the house dust mite allergen, the cat hair allergen, the dog hair allergen and the wormwood allergen freeze-dried powder are firstly re-dissolved to 5mg/mL by 20mM PBS, then the two allergens are respectively diluted to 1.5mg/mL by coating buffer solution, an NC membrane is selected, a detection line (T line) is drawn by a membrane spraying/drawing machine at 1 mu L/cm, a quality control line (C line) is drawn by goat anti-chicken IgY at 1.0mg/mL at 1 mu L/cm, and the goat anti-chicken IgY is dried for 24 hours at 37 ℃ for standby.
(3) Ball spraying: diluting anti-human IgE-second antibody marked by colloidal gold and chicken IgY with microsphere working solution according to the proportion of 1: 100, spraying the mixture on a bonding pad by using a film spraying/scribing machine, and drying for 24 hours at 37 ℃ for later use.
(4) Assembling: and assembling the sample pad, the combination pad, the chromatographic membrane and the absorbent paper on the PVC base plate in sequence for later use.
(5) Slitting: and placing the assembled large card on a cutting machine to cut the large card into test strips with the length of 4mm for later use.
The utility model discloses a use method:
(1) and selecting a test strip of the allergen to be detected, and inserting the test strip into the opening of the test paper box.
(2) Collecting fingertip blood to drip into a dripping hole of a test paper box;
(2) standing for 10-20 min, and observing the result. When two red lines (a detection line and a quality control line) appear in the observation hole, the corresponding allergen is positive; only one quality control line appears, which indicates that the corresponding allergen is negative; and if only one detection line does not have a quality control line, the detection is required to be carried out again.
Compared with the prior art, the test paper box includes two alternate segregation, each other contactless storage tanks, can detect two kinds of allergens immediately, and the doctor of being convenient for carries out the preliminary screening of allergen before the desensitization treatment, has avoided cross reaction interference experimental result between two kinds of allergens again simultaneously. The utility model discloses a scheme provides five different common allergen specificity test paper strips, can insert the test paper box according to doctor's first visit and patient's demand arbitrary selection two kinds of test paper strips, realized the individuation and the diversification that allergen definite nature detected to convenient quick, extensive applicability. Red blood cells in a whole blood sample interfere with clinical diagnostic tests and affect the accuracy of the results. Anti erythrocyte antibody in the sample pad in the scheme of test paper strip, when instiling into finger blood, the erythrocyte in the whole blood can be held back in the sample pad, realizes the erythrocyte separation in the whole blood sample. This process does not need centrifuge, also need not manual operation separation serum, great saving time has realized instant detection, also can satisfy the patient and be at by oneself and detect simultaneously, and is more convenient.
The present invention is not limited to the above embodiment, and if various modifications or variations of the present invention do not depart from the spirit and scope of the present invention, they are intended to be covered if they fall within the scope of the claims and the equivalent technology of the present invention.
Claims (10)
1. A multi-choice allergen specificity IgE quick dual detection test paper box is characterized by comprising a shell, wherein the shell forms a cavity, the cavity is provided with a baffle plate to divide the cavity into two containing grooves with the same size, one ends of the two containing grooves are respectively provided with an opening, the top surfaces of the containing grooves, close to the openings, are sequentially provided with an observation hole and a dropping hole, and the connecting line of the observation hole and the dropping hole is parallel to the baffle plate;
the test strip comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad, the colloidal gold combined pad, the nitrocellulose membrane and the absorbent paper are sequentially adhered to a polyvinyl chloride bottom plate and are partially overlapped between adjacent detection lines and quality control lines, and the test strip can be inserted into the accommodating groove from the opening;
after the test strip is inserted into the containing groove from the opening, the test strip sample pad corresponds to the dropping hole in the upper and lower directions, and the test strip nitrocellulose membrane corresponds to the observation hole in the upper and lower directions.
2. The rapid dual multi-choice allergen-specific IgE detection test paper box according to claim 1, wherein one end of the polyvinyl chloride bottom plate of the test paper strip, which is close to the absorbent paper, protrudes outwards to form a drawing end.
3. The rapid dual multi-choice allergen-specific IgE detection test paper box of any one of claims 1-2, wherein the allergen detection test paper strip comprises a dermatophagoides pteronyssinus test paper strip and a dermatophagoides farinae test paper strip.
4. The rapid dual detection test paper box for multi-choice allergen-specific IgE according to any one of claims 1-2, wherein the allergen detection test paper strip comprises a dermatophagoides pteronyssinus test paper strip, a dust mite test paper strip, a cat hair test paper strip, a dog hair test paper strip and a wormwood test paper strip.
5. The kit of any one of claims 1-4, wherein the test strip comprises a sample pad coated with anti-erythrocyte antibodies.
6. The rapid dual multi-selection allergen-specific IgE detection test paper box according to any one of claims 1-4, wherein the test paper strip comprises a colloidal gold binding pad coated with a colloidal gold-labeled anti-human IgE-secondary antibody and chicken IgY.
7. The rapid dual detection test paper box for multi-choice allergen specific IgE according to claim 3, wherein the dermatophagoides pteronyssinus test paper strip comprises a detection line coated with dermatophagoides pteronyssinus allergen and a quality control line coated with goat anti-chicken IgY.
8. The rapid dual detection test paper box for multi-choice allergen specific IgE according to claim 3, wherein the dust mite test paper strip comprises a detection line coated with dust mite allergen and a quality control line coated with goat anti-chicken IgY.
9. The multiple-choice allergen-specific IgE rapid dual detection test paper box of claim 4, wherein the cat hair test strip comprises a detection line coated with cat hair allergen and a quality control line coated with goat anti-chicken IgY, and the dog hair test strip comprises a detection line coated with dog hair allergen and a quality control line coated with goat anti-chicken IgY.
10. The multiple-choice allergen-specific IgE rapid duplex detection test paper box of claim 4, wherein the wormwood test paper strip comprises a detection line coated with wormwood allergen and a quality control line coated with goat anti-chicken IgY.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116087500A (en) * | 2022-12-26 | 2023-05-09 | 科赫生物科技（北京）有限公司 | Multi-joint detection device and application method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116087500A (en) * | 2022-12-26 | 2023-05-09 | 科赫生物科技（北京）有限公司 | Multi-joint detection device and application method thereof |
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| TR01 | Transfer of patent right |
Effective date of registration: 20220513 Address after: 400000 Chongqing Wansheng economic and Technological Development Zone high tech Industrial Park Patentee after: CHONGQING BIO-ARRAY DIAGNOSTICS CO.,LTD. Address before: 430000 people's Hospital of Wuhan University, ZhangZhidong Road, Wuchang District, Wuhan City, Hubei Province Patentee before: Tao Zezhang |
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| TR01 | Transfer of patent right |