CN116087500A - Multi-joint detection device and application method thereof - Google Patents
Multi-joint detection device and application method thereof Download PDFInfo
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- CN116087500A CN116087500A CN202211676895.1A CN202211676895A CN116087500A CN 116087500 A CN116087500 A CN 116087500A CN 202211676895 A CN202211676895 A CN 202211676895A CN 116087500 A CN116087500 A CN 116087500A
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application provides a multi-joint detection device and a use method thereof, which belong to the technical field of biological detection and comprise a detection card and a detection strip matched with the detection card, wherein the detection card comprises a first shell and a second shell, two placing grooves are formed in the second shell and are used for placing the detection strip, a sample adding hole is formed in the first shell, a flow dividing cavity is formed in the first shell and located at the position where the sample adding hole is located, a flow dividing part is arranged in the flow dividing cavity, and the center of the flow dividing part is opposite to the center of the sample adding hole; the flow distribution cavity comprises two liquid outlet holes, the two liquid outlet holes are respectively positioned at two sides of the flow distribution part, and the two liquid outlet holes are respectively communicated with the two placing grooves. The application provides a multi-joint detection device and a use method thereof, which can realize one-time sample addition detection of samples, simultaneously meet the reaction of two groups of reagent strips, and solve the pain point of the traditional multi-joint detection clamping shell; the operation is convenient, the sensitivity is high, the portable operation can be completed according to the instruction without other instruments and equipment.
Description
Technical Field
The application relates to the technical field of biological detection, in particular to a multi-joint detection device and a use method thereof.
Background
The immune colloidal gold technology is a novel immune labeling technology which uses colloidal gold as a tracer marker to be applied to antigen and antibody, mainly utilizes the characteristic that gold particles have high electron density, black brown particles are visible under a microscope at the binding site of gold-labeled proteins, and red or pink spots are visible by naked eyes when the markers are gathered in a large amount at corresponding ligands, so that the immune colloidal gold technology is used in a qualitative or semi-quantitative rapid immune detection method, and the reaction can be amplified through the deposition of silver particles, which is called immune gold-silver staining.
The total IgE detection is a detection means for judging the allergic constitution of the organism, and is also an important sign of allergy, and when the total IgE is increased, the patient is in an allergic state. However, the total IgE elevation may also be due to a variety of other factors, such as: respiratory diseases, parasites, tumors, etc. Thus, total IgE is not the most reliable basis for determining allergen and degree of allergy. The specific IgE can accurately screen and detect the allergen causing the disease, is the most specific diagnosis of the allergic disease, and simply speaking, the specific IgE can accurately detect the value of each allergen, which has very important guiding significance for clinical treatment.
Disclosure of Invention
The application provides a multi-joint detection device and a use method thereof, so as to solve the problems that single sample adding of a colloidal gold chromatography product in the current market is difficult to carry out multi-joint detection and the multi-joint detection project is seriously interfered.
A first aspect of an embodiment of the present application provides a multi-joint detection device, including: a test card and a test strip adapted to the test card;
the detection card comprises a first shell and a second shell, wherein two placing grooves are formed in the second shell and are used for placing the detection strips;
the sample adding device comprises a first shell, a second shell, a first shell and a second shell, wherein a sample adding hole is formed in the first shell, a flow dividing cavity is formed in the position, where the sample adding hole is located, of the first shell, a flow dividing part is arranged in the flow dividing cavity, and the center of the flow dividing part is opposite to the center of the sample adding hole;
the flow distribution cavity comprises two liquid outlet holes, the two liquid outlet holes are respectively positioned at two sides of the flow distribution part, and the two liquid outlet holes are respectively communicated with the two placing grooves.
Optionally, the side walls on two sides of the first shell are provided with exhaust holes, and the positions of the two exhaust holes respectively correspond to the positions of the two liquid outlet holes.
Optionally, two observation windows are arranged on the first shell;
wherein, two the observation windows respectively with two the standing groove one-to-one sets up.
Optionally, the detection strip comprises a first end and a second end, and when the detection strip is positioned in the placing groove, the position of the first end corresponds to the position of the liquid outlet hole;
the detection strip comprises a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the absorbent paper are arranged on the bottom plate in sequence along the direction from the first end to the second end;
wherein, the nitrocellulose membrane is coated with a plurality of detection lines and quality control lines.
Optionally, the position of the nitrocellulose membrane corresponds to the position of the viewing window.
Optionally, a plurality of detection lines are coated with a plurality of allergen antigens, and the quality control line is coated with goat anti-chicken IgY antibodies;
wherein each detection line is coated with an allergen antigen, and the types of allergen antigens coated on each detection line are different.
Optionally, the plurality of allergen antigens includes an inhalant allergen antigen and an inhalant allergen antigen;
the inhalant allergen comprises one of the following: cat dander, mugwort, birch, timothy, cockroach, dog dander, plantain, ragweed, house dust mite and alternaria;
the food-grade allergen comprises one of the following: peanut, milk, cod, scallop, chicken protein, shrimp, crab, soybean, wheat and almond.
Optionally, the colloidal gold pad-coated antibodies are colloidal gold particles-mouse anti-human IgE antibodies and colloidal gold particles-chicken IgY antibodies.
The second aspect of the embodiments of the present application provides a method for using a multi-joint detection device, where the detection device includes a detection card and a detection strip, and a sample loading hole is provided on the detection card, and the method for using the multi-joint detection device includes:
adding the detection sample into the sample adding hole;
detection information generated by the detection strip according to the detection sample is determined.
Optionally, the test sample is a whole blood sample or a plasma sample or a serum sample.
The beneficial effects of this application:
1) The method and the device realize the purpose of rapidly detecting allergens (including inhaled allergens or food allergens) causing various allergic reactions to human bodies technically, can clearly and specifically detect the allergens, and are beneficial to rapid detection of doctors and allergen self-check of patients. The single-hole single sample adding is convenient to operate, detection samples do not need to be acquired for multiple times, the detection device is high in sensitivity and portable, operation can be completed according to instructions without the assistance of other instruments and equipment;
2) The application provides a special detection card shell, which can simultaneously meet the detection of two detection strips by one sample adding detection sample, and solves the problem of pain points of the traditional multi-joint detection card shell, which need multiple sample adding;
3) The application is applicable to whole blood detection, and is particularly applicable to self-detection of infants and families.
Drawings
FIG. 1 is a schematic diagram of a detecting card in a multi-joint detecting device according to an embodiment of the present disclosure;
FIG. 2A is a cross-sectional view of the well A-A of FIG. 1;
FIG. 2B is an enlarged partial schematic view of FIG. 2A;
FIG. 3A is a schematic view of the B-B orientation of the first housing of the test card of FIG. 1;
FIG. 3B is an enlarged partial schematic view of FIG. 3A;
FIG. 4 is a front perspective view of FIG. 1;
FIG. 5 is a schematic diagram of a test strip structure of a multi-joint test device according to an embodiment of the present disclosure;
fig. 6 is a flowchart illustrating a method for using a multi-joint detection device according to an embodiment of the present application.
Reference numerals: 101 a first housing; 103, an observation window; 104, a sample adding hole; 105 split-flow sections; 106, a liquid outlet hole; 107 exhaust holes; a bottom plate 201; 202 sample pad; 203 a colloidal gold pad; 204 nitrocellulose membrane; 205 quality control lines; 206 detection line; 207 of water absorbing paper.
Detailed Description
The technical solutions of the present application are further described below by specific examples, which do not represent limitations on the scope of protection of the present application. Some insubstantial modifications and adaptations of the invention by others in light of the teachings of this application are still within the scope of this application.
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the invention briefly described above will be rendered by reference to specific embodiments that are illustrated in the appended drawings.
Example 1
As shown in fig. 1-5, a multi-joint detection device of the present embodiment includes a detection card 100 and a detection strip 200 adapted to the detection card.
As shown in fig. 1-4, a test card of a multi-joint test device according to the present embodiment is shown.
The test card 100 includes a first housing 101 and a second housing 102 (not shown in the drawings), and the first housing 101 is provided with a sample loading hole 104, and the sample loading hole 104 is used for loading a test sample in a single time.
The first housing 101 is provided with a flow distribution cavity at the position of the sample adding hole 104, a flow distribution part 105 is arranged in the flow distribution cavity, and the flow distribution part 105 is used for distributing the detection sample added into the sample adding hole 104 into two parts.
The center of the flow dividing part 105 is opposite to the center of the sample adding hole 104, the flow dividing cavity comprises two liquid outlet holes 106, the two liquid outlet holes 106 are respectively located at two sides of the flow dividing part 105, and after the flow dividing part 105 divides the detection sample into two parts, the two parts of detection samples can flow out from the two liquid outlet holes 106 respectively.
Meanwhile, the second housing 102 is provided with two placement grooves (not labeled in the figure) for placing the test strips 100; when the first shell 101 and the second shell 102 are connected, the two liquid outlet holes 106 are respectively communicated with the two placing grooves, so that the split detection samples can directly enter the placing grooves and are directly used for detection of the detection strips, the loss of the detection samples is avoided, and the addition amount of the detection samples can be reduced.
The first housing 101 is further provided with a buffer area (not labeled in the figure) of the sample loading hole 104. Because the flow of the detection sample and the sample diluent needs a certain time, the buffer area can be used for buffering the detection sample and the sample diluent after being added into the sample adding hole.
Preferably, the buffer area and the sample adding hole 104 form a circular truncated cone structure, and the sample adding hole 104 is a circle with smaller diameter in the circular truncated cone structure; the diameter of the sample adding hole is 11.6mm, and the diameter of the liquid outlet hole is 2.46mm.
Preferably, the diverting portion is a wedge-shaped member comprising two inclined surfaces, and the inclined directions of the two inclined surfaces are symmetrical about the center of the diverting portion, and the angle of the inclined surfaces to the vertical is preferably 110 °.
Preferably, the material of the shunt part is one of ABS (Acrylonitrile Butadiene Styrene ) plastic, PS (polystyrene) plastic, HIPS (High impact polystyrene ) plastic, PC (Polycarbonate) plastic.
Because the detection sample is a blood sample, including a whole blood sample, a plasma sample or a serum sample, the relative viscosity of the blood sample is higher than that of water, so that the blood sample is not easy to flow downwards when being added into the sample adding hole. Therefore, in order to improve the flowability of the detection sample, in the embodiment of the present application, the exhaust holes 107 are formed on the side walls of both sides of the first housing 101, and the positions of the two exhaust holes 107 respectively correspond to the positions of the two liquid outlet holes 106. Therefore, after the detection sample is added, the air in the liquid outlet hole 106 is discharged through the air outlet hole 107, so that the detection sample flows smoothly, and the sample adding hole 104 is not blocked.
Meanwhile, two observation windows 103 are arranged on the first shell 101, and the two observation windows 103 are respectively arranged in one-to-one correspondence with the two placing grooves, so that a user can observe the specific condition of the detection strip conveniently.
After the test card 100 is assembled, it is sealed and stored in an aluminum foil bag containing a desiccant.
Fig. 5 shows a test strip in a multi-joint test device according to this embodiment.
The test strip 200 includes a first end and a second end, the test strip 200 being positioned in the placement slot in use, and the first end being positioned to correspond to the position of the exit aperture 106.
The test strip 200 comprises a bottom plate 201, and a sample pad 202, a colloidal gold pad 203, a nitrocellulose membrane 204 and a water absorbing paper 207 which are arranged on the bottom plate, wherein the sample pad 202, the colloidal gold pad 203, the nitrocellulose membrane 204 and the water absorbing paper 207 are sequentially arranged on the bottom plate 201 along the direction from a first end to a second end, namely, the sample pad 202 and the liquid outlet hole 106 are correspondingly arranged, so that a test sample can flow out from the liquid outlet hole 106 and can be absorbed by the sample pad 202, and loss of the test sample is reduced.
It should be noted that, the first end and the second end referred to in this application are defined according to the flowing direction of the detection sample, and in the using process of the detection strip, the detection sample flows from the sample end to the direction of the absorbent paper through the chromatographic action, the sample pad end is located at the position of the first end, and the absorbent paper end is located at the position of the second end.
Preferably, the sample pad 202 is a polyester fiber film or a glass cellulose film.
Preferably, the colloidal gold pad 203 is a glass cellulose film.
Preferably, the nitrocellulose membrane 204 is coated with a plurality of detection lines 206 and a quality control line 205. When the detection line is used, the detection line is used for displaying the detection result of the multi-joint detection device, and the quality control line is used for displaying whether the detection result of the multi-joint detection device is effective. In this application embodiment, the detection line is provided with five altogether, and five detection lines are close to the even interval setting of length direction of first end along the detection strip, and the matter control line setting is in the position that is close to the second end.
Wherein, the position of the nitrocellulose membrane corresponds to the position of the observation window, so that a user can observe the detection result of the detection device.
Wherein, five detection lines are coated with various allergen antigens, and the quality control line is coated with goat anti-chicken IgY antibody. Allergen antigen coated on the detection line can be combined with colloidal gold particles-mouse anti-human IgE antibodies to display color, and the result of the detection line can be observed with naked eyes; the sheep anti-chicken IgY antibody coated by the quality control line can be respectively combined with the colloidal gold particle-mouse anti-human IgE antibody and the colloidal gold particle-chicken IgY antibody, and can be red, so that the normal detection of the detection strip is ensured.
Wherein each detection line is coated with an allergen antigen, and the types of allergen antigens coated on each detection line are different.
Preferably, the plurality of allergen antigens includes an inhalant allergen antigen and an inhalant allergen antigen.
Preferably, the inhaled allergen comprises one of the following: cat dander, mugwort, birch, timothy, cockroach, dog dander, plantain, ragweed, house dust mite and alternaria.
Preferably, the food-grade allergen comprises one of the following: peanut, milk, cod, scallop, chicken protein, shrimp, crab, soybean, wheat and almond.
Illustratively, the plurality of detection lines includes a first detection line, a second detection line, a third detection line, a fourth detection line, and a fifth detection line, wherein the first detection line is coated with a cat dander crude extract antigen, the second detection line is coated with a peanut crude extract antigen, the third detection line is coated with a birch crude extract antigen, the fourth detection line is coated with an egg white crude extract antigen, and the fifth detection line is coated with a cockroach crude extract antigen. Those skilled in the art may set the settings according to the needs, and the embodiments of the present application are not repeated.
Preferably, the concentration of the desired coated allergen antigen is 0.1-3 mg/mL. Illustratively, the concentration of the coated allergen antigen may be 0.1mg/mL, 0.2mg/mL, 0.5mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 2.5mg/mL, 3mg/mL, and the like. Those skilled in the art may set the settings according to the needs, and the embodiments of the present application are not repeated.
Preferably, the concentration of the required coated goat anti-chicken IgY antibody is 1-3 mg/mL. Illustratively, the coated goat anti-chicken IgY antibody concentration may be 1mg/mL, 1.5mg/mL, 2mg/mL, 2.5mg/mL, 3mg/mL, and the like. Those skilled in the art may set the settings according to the needs, and the embodiments of the present application are not repeated.
Preferably, the colloidal gold pad-coated antibodies are colloidal gold particles-murine anti-human IgE antibodies and colloidal gold particles-chicken IgY antibodies.
Preferably, the colloidal gold particles coated by the colloidal gold pad, the mouse anti-human IgE antibody and the colloidal gold particles, the chicken IgY antibody are mixed according to the proportion of 10:1-3:1. Illustratively, the ratio of colloidal gold particles-murine anti-human IgE antibodies to colloidal gold particles-chicken IgY antibodies may be 10:1, 8:1, 5:1, 3:1, etc. Those skilled in the art may set the settings according to the needs, and the embodiments of the present application are not repeated.
Preferably, the concentration of the colloidal gold particles-mouse anti-human IgE antibody and the colloidal gold particles-chicken IgY antibody coated on the colloidal gold pad is 5-25 mug/mL. Illustratively, the concentrations of the colloidal gold particle-murine anti-human IgE antibody and the colloidal gold particle-chicken IgY antibody may be 5 μg/mL, 10 μg/mL, 15 μg/mL, 25 μg/mL, etc. Those skilled in the art may set the settings according to the needs, and the embodiments of the present application are not repeated.
Specific methods of preparing the test strip may include: attaching a coated nitrocellulose membrane 204 to the middle of the base plate 201; one end of the water absorbing paper 207 is attached to the second end of the bottom plate, and the other end of the water absorbing paper 207 is covered on the nitrocellulose membrane 204; one end of the colloidal gold pad 203 is covered on the nitrocellulose membrane 204 close to the detection line 206, and the other end is stuck on the bottom plate 201; one end of the sample pad 202 is stuck on the first end of the bottom plate 201, and the other end is covered on the colloidal gold pad 203; the strip is cut into strips 3mm wide by a cutter to obtain the test strip 200 of the multi-joint test device.
The embodiment also discloses a multi-joint detection device which comprises the detection card and the detection strip and sample diluent, wherein the sample diluent is matched with a detection sample for use when the multi-joint detection device is used, and the sample diluent is added after the detection sample is added. The sample diluent is helpful for preventing the sample to be detected from coagulating too fast, so as to avoid detection failure; meanwhile, the sample diluent is favorable for diluting the concentration of the detection sample, and the detection accuracy is improved.
The components of the sample diluent comprise buffer solution, macromolecular polymer, surfactant and preservative. The components added in the sample diluent are helpful for stabilizing the space structure of the antibody and improving the chromatographic effect of the blood sample on the nitrocellulose membrane.
Preferably, the buffer is at least one of PBS (phosphate buffered saline ), tris (Tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane), BBS (Borate Buffered Salin, borate buffer); the macromolecular polymer is at least one of PEG (Polyethylene glycol ), PVP (polyvinyl pyrrolidone, polyvinylpyrrolidone) and PVA (Poly (vinyl alcohol), polyvinyl alcohol); the surfactant is at least one of triton and tween; the preservative is NaN 3 At least one of Sodium azide and PC-300 (ProClin 300 ). The present example also discloses the composition of the sample pad treatment fluid in the test strip.
The components of the sample pad treatment fluid include buffer solution, macromolecular polymer, surfactant, preservative, blocking agent and substances which are beneficial to the whole blood sample treatment. When the component added in the sample pad treatment liquid is used for detecting that the sample is a whole blood sample, the method is beneficial to filtering large particulate matters in the sample detection sample, enhancing specific reaction, reducing interference of endogenous matters in the detection sample and improving the chromatography effect of the detection sample on the nitrocellulose membrane.
Preferably, the blocking agent is at least one of a human anti-animal antibody (e.g., human anti-murine antibody) blocking agent, a rheumatoid factor blocking agent, a xenotropic antibody blocking agent, a complement blocking agent, an autoantibody blocking agent; the processing substance beneficial to the whole blood sample is at least one of anti-erythrocyte antibody and phytohemagglutinin. The embodiment also discloses components of the colloidal gold pad treatment fluid in the detection strip, which comprise buffer solution, macromolecular polymer, surfactant, preservative and saccharides. The components added in the colloidal gold pad treatment liquid are helpful for improving the sensitivity of the antibody and the stability of the antibody in a solid phase.
Preferably, the saccharide is at least one of trehalose, sucrose, and dextran.
Preferably, the concentration of buffer solution in the sample diluent, the sample pad treatment solution and the colloidal gold pad treatment solution is 10-200 mM, the concentration of macromolecular polymer is 0.1-5%, the concentration of surfactant is 0.1-3%, the concentration of preservative is 0.01-2%, the concentration of substances beneficial to the whole blood sample treatment is 10-50 mug/mL, and the concentration of saccharides is 0.5-5%.
After the sample pad and the colloidal gold pad on the detection strip in the detection device are subjected to the optimization treatment of the sample pad treatment liquid and the colloidal gold pad treatment liquid, the detection device is optimized in the positive new coincidence rate and the negative coincidence rate of the allergen detection. Different inhalant allergens and food allergens are coated in the detection device (the multi-joint detection device which is not optimized by the bonding pad treatment liquid and the multi-joint detection device which is optimized by the bonding pad treatment liquid) and the detection results are shown in the table 1 and the table 2, and the results show that the positive coincidence rate and the negative coincidence rate detected by the detection device accord with the industry standard, and the positive coincidence rate and the negative coincidence rate of the detection device after the optimization treatment are higher than the positive coincidence rate and the negative coincidence rate of the detection device after the non-optimization treatment.
TABLE 1 serum sample test results (1)
TABLE 2 serum sample test results (2)
The macromolecular polymers added in the sample diluent, the sample pad treatment liquid and the colloidal gold pad treatment liquid can improve the activity of the antibody and provide a space framework for the antibody; the blocker is helpful for enhancing the specific reaction, reducing the interference of endogenous substances in the sample and reducing the interference caused by detecting the non-target protein antibodies in the sample; substances which are favorable for the whole blood sample treatment can filter large granular substances in the detection sample, so that the detection sample smoothly passes through the nitrocellulose membrane; the preservative is helpful for the stabilization of the sample pad and the colloidal gold pad. Therefore, the positive coincidence rate and the negative coincidence rate of the detection device can be improved by combining the detection sample treated by the sample diluent with the detection strip after the optimization treatment.
Example two
FIG. 6 is a flow chart showing the steps of a method of using a multiplexed detection apparatus.
Referring to fig. 6, the embodiment further discloses a method for using the multi-joint detection device, where the method includes:
step 601: the test sample is added to the sample addition well.
Specifically, the detection sample is a blood sample, and is a whole blood sample, a plasma sample or a serum sample.
Specifically, the detectable allergens include: cat dander, mugwort, white birch, timothy, cockroach, dog dander, plantain, ragweed, house dust mite, alternaria, peanut, milk, cod, scallop, chicken protein, shrimp, crab, soybean, wheat, almond.
Step 602: detection information generated by the test strip from the test sample is determined.
Specifically, 60 μl of the detection sample is added from the sample addition Kong Di, 60 μl of the sample diluent is added dropwise, and then the detection sample and the sample diluent flow to the sample pads of the two test strips in the detection card through the flow dividing part.
Further, the flow dividing part divides the detection sample and the sample diluent into two parts, and each part of the detection sample and the sample diluent flows to the liquid outlet through two inclined planes of the flow dividing part.
Preferably, the two test strips disposed within the second housing are separated from each other; each test sample and sample diluent enables two test strips to simultaneously perform an immunochromatographic reaction.
Preferably, the detection device is capable of simultaneously performing detection of ten different allergens.
The detection device in the application only needs to carry out single-hole single sample addition, does not need to collect samples of testers and sample addition for many times, can detect ten different allergens at one time, and is simple and convenient to operate and high in practicality.
The application adopts high-specificity IgE antibody and antigen reaction, and fixes colloidal gold particles-mouse anti-human IgE antibody and colloidal gold particles-chicken IgY antibody on a colloidal gold pad. Meanwhile, by combining an immunochromatography technology, after a detection sample is diluted by a sample diluent, the detection sample is combined with colloidal gold particles coated on a colloidal gold pad-mouse anti-human IgE antibody to form a marker in the flowing process, and is combined with an antigen coated on a detection line to form macroscopic red or pink spots. Finally, the goat anti-chicken IgY antibody coated on the quality control line can be combined with the rest of the colloidal gold particle-mouse anti-human IgE antibody and the colloidal gold particle-chicken IgY antibody to form macroscopic red or pink spots. Since the colloidal gold particle-labeled antibodies are aggregated in a large amount at the corresponding ligands, color development is finally performed at the allergen antigen coated on the detection line on the nitrocellulose membrane, and detection results of the multi-joint detection device can be observed by a detector through an observation window.
The detection strip of the multi-detection device in the application is coated with a plurality of detection lines, after a detection sample is combined with the colloidal gold particles-mouse anti-human IgE antibodies, the detection sample is combined with allergen antigens in the detection lines, and the following situations may occur: 1) The residual colloidal gold particles flowing to the quality control line, namely the mouse anti-human IgE antibody, have more content, so that the quality control line is colored; 2) The colloidal gold particles flowing to the quality control line, namely the mouse anti-human IgE antibody, have too little or no content, so that the quality control line does not develop color. Therefore, in order to avoid the second situation, the colloidal gold pad is coated with the colloidal gold particle-mouse anti-human IgE antibody and also coated with the colloidal gold particle-chicken IgY antibody. When the second condition appears, the goat anti-chicken IgY antibody coated on the quality control line can still be combined with the colloidal gold particle-chicken IgY antibody to develop color, so that the effective (negative or positive) detection result of the detection device is ensured. Therefore, the multi-joint detection device prepared by the application not only can detect the specificity of a plurality of different allergen antigens at the same time, but also can avoid the invalid condition of the detection strip in the detection device, and reduce the condition of repeated detection.
The test strip test results were analyzed as follows:
positive: when the quality control line has red stripes, the detection line also has red stripes, and the detection line is judged to be positive, which indicates that the detection result is positive for IgE antibodies corresponding to the detection line in the detection sample;
negative: when the quality control line has red stripes, any detection line is not red, and is judged to be negative, wherein the detection result is that the IgE antibody corresponding to the detection line in the detection sample is negative;
invalidation: when the quality control line does not appear red, whether the detection line has red stripes or not indicates that the detection result is invalid.
The above description is provided for the detailed description of a multi-joint detection device and its use method, and specific examples are applied to the description of the principles and embodiments of the present application, where the description of the above examples is only for helping to understand the method and core ideas of the present application; meanwhile, as those skilled in the art will have modifications in the specific embodiments and application scope in accordance with the ideas of the present application, the present description should not be construed as limiting the present application in view of the above.
Claims (10)
1. A multiplexed detection apparatus, comprising:
a test card and a test strip adapted to the test card;
the detection card comprises a first shell and a second shell, wherein two placing grooves are formed in the second shell and are used for placing the detection strips;
the sample adding device comprises a first shell, a second shell, a first shell and a second shell, wherein a sample adding hole is formed in the first shell, a flow dividing cavity is formed in the position, where the sample adding hole is located, of the first shell, a flow dividing part is arranged in the flow dividing cavity, and the center of the flow dividing part is opposite to the center of the sample adding hole;
the flow distribution cavity comprises two liquid outlet holes, the two liquid outlet holes are respectively positioned at two sides of the flow distribution part, and the two liquid outlet holes are respectively communicated with the two placing grooves.
2. The multi-joint detection device according to claim 1, wherein the side walls on both sides of the first housing are provided with exhaust holes, and positions of the two exhaust holes correspond to positions of the two liquid outlet holes respectively.
3. The multiple sensing device according to claim 1, wherein two viewing windows are provided on the first housing;
the two observation windows are respectively arranged in one-to-one correspondence with the two placing grooves.
4. The multiplexed detection device of claim 1, wherein the test strip includes a first end and a second end, the first end being positioned to correspond to the position of the exit aperture when the test strip is positioned within the placement slot;
the detection strip comprises a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and absorbent paper, wherein the sample pad, the colloidal gold pad, the nitrocellulose membrane and the absorbent paper are arranged on the bottom plate in sequence along the direction from the first end to the second end;
wherein, the nitrocellulose membrane is coated with a plurality of detection lines and quality control lines.
5. The multiplexed detection device of claim 4, wherein the nitrocellulose membrane is positioned to correspond to the position of the viewing window.
6. The multiplexed detection device of claim 4, wherein a plurality of the detection lines are coated with a plurality of allergen antigens and the quality control lines are coated with goat anti-chicken IgY antibodies;
wherein each detection line is coated with an allergen antigen, and the types of allergen antigens coated on each detection line are different.
7. The multiplexed detection device of claim 6, wherein a plurality of the allergen antigens include an inhalant allergen antigen and an eating allergen antigen;
the inhalant allergen comprises one of the following: cat dander, mugwort, birch, timothy, cockroach, dog dander, plantain, ragweed, house dust mite and alternaria;
the food-grade allergen comprises one of the following: peanut, milk, cod, scallop, chicken protein, shrimp, crab, soybean, wheat and almond.
8. The multiplex assay device as defined in claim 4 wherein the colloidal gold pad coated antibodies are colloidal gold particle-murine anti-human IgE antibodies and colloidal gold particle-chicken IgY antibodies.
9. The application method of the multi-joint detection device comprises the steps of detecting a card and a detection strip, wherein a sample adding hole is formed in the detection card, and the application method comprises the following steps:
adding the detection sample into the sample adding hole;
detection information generated by the detection strip according to the detection sample is determined.
10. The method of claim 9, wherein the test sample is a whole blood sample or a plasma sample or a serum sample.
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