CN208833770U - A kind of three joint inspections survey card - Google Patents
A kind of three joint inspections survey card Download PDFInfo
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- CN208833770U CN208833770U CN201821581978.1U CN201821581978U CN208833770U CN 208833770 U CN208833770 U CN 208833770U CN 201821581978 U CN201821581978 U CN 201821581978U CN 208833770 U CN208833770 U CN 208833770U
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- sample
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- card
- bottom plate
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Abstract
The utility model discloses a kind of three joint inspections to survey card, it gets stuck intracorporal bottom plate including detection casing clamping body and setting in detection, a sample cell and 3 observation windows are offered on the detection casing clamping body, the first filter layer is equipped in the sample cell, bottom is equipped with the second filter layer, the substrate is equipped with detection card, the detection card is by sample pad, gold conjugation pad, chromatographic film and water-absorbent material composition, it is fixed on bottom plate on the left of the sample pad, and right side overlaps on top on the left of gold conjugation pad, top on the left of chromatographic film is overlapped on the right side of the gold conjugation pad, top on the right side of chromatographic film is overlapped on the left of the water-absorbent material, and right side is fixed on bottom plate.Three detections of the utility model, which block, shares a sample cell and sample pad, only need to disposably be loaded the three kinds of projects to be checked that can survey, operate more convenient, pass through setting strainer and filter membrane, it plays a role in filtering, reduces the use of filter device or centrifuge, be suitable for field test.
Description
Technical field
The utility model relates to field of biotechnology, more particularly to a kind of three joint inspections to survey card.
Background technique
Colloidal gold immunity chromatography is to be fixed on the antigen of specificity or antibody on film with ribbon, colloid gold label examination
Agent (antibody or monoclonal antibody) is adsorbed on bonding pad, after sample to be examined is added in the sample pad of test strips one end, is passed through
Capillarity moves forward, and reacts to each other after dissolving the colloid gold label reagent on bonding pad, then be moved to fixed antigen or
When the region of antibody, the conjugate of object and gold marked reagent to be checked occurs specific binding therewith again and is trapped, and is gathered in detection
It takes, then observes by the naked eye colour developing as a result, to realize the qualitative or quantitative analysis to determinand.
This method need to use colloidal gold chromatographic detection card, and existing colloidal gold chromatographic detection blocks only one detection window,
The corresponding sample cell of each detection window, can only detect unitem, when a kind of sample to be tested needs to detect a variety of projects
When index, need repeatedly to sample sample-adding, it is more inconvenient, and some samples to be tested usually contain the impurity such as precipitating or muddy object,
It could be detected with colloidal gold method after needing filtering or centrifugal treating, it has not been convenient to on-site test.
Summary of the invention
The purpose of this utility model is that: a kind of project can only be once detected for existing colloidal gold chromatographic detection card,
The problem very inconvenient for the sample detection containing the impurity such as precipitating or muddy object, the utility model provide a kind of three joint inspections survey
Card.
The technical solution adopted in the utility model is as follows:
Card is surveyed in a kind of three joint inspections, including detection casing clamping body and setting are got stuck intracorporal bottom plate in detection, and the detection is got stuck
A sample cell and 3 observation windows are offered on body, the first filter layer is equipped in the sample cell, and bottom is equipped with the second filtering
Layer, the substrate are equipped with detection card, and the detection card is by sample pad, gold conjugation pad, chromatographic film and water-absorbent material group
At the sample pad left side is fixed on bottom plate, and right side overlaps on top on the left of gold conjugation pad, and the colloidal gold combines
Pad right side overlaps on top on the left of chromatographic film, top on the right side of chromatographic film is overlapped on the left of the water-absorbent material, and right side is fixed on
On bottom plate.
The working principle of the utility model are as follows:
Sample to be detected is added drop-wise to sample after the filtration of the first filter layer and the second filter layer in sample cell
On pad, then spread to the direction of observation window, the object to be detected in sample in gold conjugation pad colloid gold label it is special
Property monoclonal antibody combine and together to the direction of observation window diffusion, when colloid gold particle diffuses to detection line with sample solution
When, because object to be detected is combined with the monoclonal antibody specific of colloid gold label, to inhibit the special of colloid gold label
Property monoclonal antibody and chromatographic film on specific support protein conjugate in detection line combination, and then inhibit detection line colour developing.
With the difference of object to be detected content in the sample, detection line will appear the area that colour developing is strong and weak and whether develops the color
Not, it may determine that the content of object to be detected in the sample relative to setting relative to the difference that nature controlling line develops the color according to detection line
The height for detecting limit, when not having the concentration of object to be detected or object to be detected to prescribe a time limit in sample lower than detection, detection line colour developing is greater than
Nature controlling line, result judgement are feminine gender, are prescribed a time limit when the concentration of object to be detected in sample is equal to detection, detection line colour developing is equal to Quality Control
Line, result judgement are weakly positive, are prescribed a time limit when the concentration of object to be detected in sample is higher than detection, and detection line colour developing is less than nature controlling line,
Result judgement is the positive.
Further, the bottom plate is PVC board, and by being made of non-woven fabrics, gold conjugation pad is made sample pad of fiber,
Chromatographic film is nitrocellulose filter, and water-absorbent material is blotting paper.It is abundant that the sample pad made of non-woven fabrics can will test liquid
It absorbs, is sufficiently reacted with the gold labeling antibody full contact on gold conjugation pad.
Further, the detection card has 3, and shares a sample pad and a sample cell, shares a sample cell
Sample-adding number can be reduced, when same sample needs to detect a variety of projects, can be added at one time in sample pad, then flow
Onto 3 gold conjugation pads.
Further, the sample cell is funnel-form, and first filter layer is strainer, and the second filter layer is filter membrane, filter
Net can filter out biggish impurity in sample, filter membrane can further the lesser substance such as crystallization in filtered sample and compared with
Small impurity.
Further, the observation window is separately positioned on above corresponding chromatographic film, and is equipped with beside each observation window pair
The number answered, number can be convenient differentiation data, will not obscure.
In conclusion by adopting the above-described technical solution, the beneficial effects of the utility model are:
1. three detections of the utility model, which block, shares a sample cell and sample pad, only need to disposably be loaded can survey three
Kind project to be checked, simplifies step while having saved sample, operate more convenient.
2. filter membrane is arranged by the way that strainer is arranged in sample cell, in sample trench bottom in the utility model, sample is allowed to be added dropwise
It is once filtered before to sample pad, the impurity inside sample is removed, avoids the impurity in sample to testing result
Interference reduces the trouble using filter device or centrifuge, is applicable not only to laboratory, is more suitable for field test, solves
The problem that detecting field is limited.
Detailed description of the invention
The utility model will illustrate by example and with reference to the appended drawing, in which:
Fig. 1 is the structural schematic diagram that card is surveyed in three joint inspection of the utility model；
Fig. 2 is the side view that card is surveyed in three joint inspection of the utility model；
Fig. 3 is that card internal structure top view is surveyed in three joint inspection of the utility model.
In the figure, it is marked as 1- detects casing clamping body, 2- sample cell, 3- observation window, 4- detection line, 5- nature controlling line, 6- number, 7-
Detection card, 8- bottom plate, the first filter layer of 9-, the second filter layer of 10-, 11- sample pad, 12- gold conjugation pad, 13- chromatographic film,
14- water-absorbent material.
Specific embodiment
All features disclosed in this specification can be with any other than mutually exclusive feature and/or step
Mode combines.
It elaborates with reference to the accompanying drawing to the utility model.
Embodiment 1
A kind of three joint inspections survey card, including detection casing clamping body 1 and the bottom plate 8 being arranged in detection casing clamping body 1, the detection blocks
It offers sample cell 2 and 3 observation window 3 on shell 1, is equipped with the first filter layer 9 in the sample cell 2, bottom is equipped with the
Two filter layers 10, the bottom plate 8 are equipped with detection card 7 above, and the detection card 7 is by sample pad 11, gold conjugation pad 12, chromatography
Film 13 and water-absorbent material 14 form, and are fixed on bottom plate 8 on the left of the sample pad 11, and right side overlaps on gold conjugation pad 12
Above left side, 13 left side top of chromatographic film is overlapped on the right side of the gold conjugation pad 12, is overlapped on the left of the water-absorbent material 14
Above 13 right side of chromatographic film, and right side is fixed on bottom plate 8.
Sample to be detected is added drop-wise to after the filtration of the first filter layer 9 and the second filter layer 10 in sample cell 2
It in sample pad 11, is then spread to the direction of observation window 3, colloidal gold mark in the object to be detected and gold conjugation pad 12 in sample
The monoclonal antibody specific of note is combined and is spread together to the direction of observation window 3, when colloid gold particle is spread with sample solution
When to detection line 4, because object to be detected is combined with the monoclonal antibody specific of colloid gold label, to inhibit colloidal gold mark
The combination of specific support protein conjugate on the monoclonal antibody specific and chromatographic film 13 of note in detection line 4, and then inhibit
Detection line 4 develops the color.
With the difference of object to be detected content in the sample, detection line 4 will appear the area that colour developing is strong and weak and whether develops the color
Not, it may determine that the content of object to be detected in the sample relative to setting relative to the difference that nature controlling line 5 develops the color according to detection line 4
Detection limit height, when do not have in sample the concentration of object to be detected or object to be detected lower than detection prescribe a time limit, detection line 4 develops the color greatly
In nature controlling line 5, result judgement is feminine gender, is prescribed a time limit when the concentration of object to be detected in sample is equal to detection, the colour developing of detection line 4 is equal to matter
Line 5 is controlled, result judgement is weakly positive, is prescribed a time limit when the concentration of object to be detected in sample is higher than detection, the colour developing of detection line 4 is less than Quality Control
Line 5, result judgement are the positive.
Embodiment 2
On the basis of embodiment 1, the bottom plate 8 is PVC board, and by being made of non-woven fabrics, colloidal gold combines sample pad 11
Pad 12 is made of fiber, and chromatographic film 13 is nitrocellulose filter, and water-absorbent material 14 is blotting paper.The sample pad made of non-woven fabrics
11, which can will test liquid, fully absorbs, and sufficiently reacts with the gold labeling antibody full contact on gold conjugation pad 12.
Embodiment 3
On the basis of embodiment 1, the detection card 7 has 3, and shares a sample pad 11 and a sample cell 2, altogether
Sample-adding number can be reduced with a sample cell 2, when same sample needs to detect a variety of projects, sample can be added at one time
On product pad 11, then it flow on 3 gold conjugation pads 12.
Embodiment 4
On the basis of embodiment 1, the sample cell 2 is funnel-form, and first filter layer 9 is strainer, the second filtering
Layer 10 is filter membrane, and strainer can filter out biggish impurity in sample, filter membrane can further crystallization in filtered sample etc. compared with
Small substance and lesser impurity.
Embodiment 5
On the basis of embodiment 1, the observation window 3 is separately positioned on corresponding 13 top of chromatographic film, and each observation
Corresponding number 6 is equipped with beside window 3, number 6 can be convenient differentiation data, will not obscure.
Claims (5)
1. card is surveyed in a kind of three joint inspections, the bottom plate (8) including detection casing clamping body (1) and setting in detection casing clamping body (1), feature
It is, a sample cell (2) and 3 observation windows (3) is offered on the detection casing clamping body (1), is equipped in the sample cell (2)
First filter layer (9), bottom are equipped with the second filter layer (10), and the bottom plate (8) is equipped with detection card (7), the detection card above
(7) it is made of sample pad (11), gold conjugation pad (12), chromatographic film (13) and water-absorbent material (14), the sample pad (11)
Left side is fixed on bottom plate (8), and right side overlaps on top, the gold conjugation pad (12) on the left of gold conjugation pad (12)
Right side overlaps on top on the left of chromatographic film (13), and top on the right side of chromatographic film (13) is overlapped on the left of the water-absorbent material (14), and
Right side is fixed on bottom plate (8).
2. card is surveyed in a kind of three joint inspection according to claim 1, which is characterized in that the bottom plate (8) is PVC board, sample pad
(11) by being made of non-woven fabrics, gold conjugation pad (12) is made of fiber, and chromatographic film (13) is nitrocellulose filter, water-absorption material
Expect that (14) are blotting paper.
3. card is surveyed in a kind of three joint inspection according to claim 1, which is characterized in that the detection card (7) has 3, and shares
One sample pad (11) and a sample cell (2).
4. card is surveyed in a kind of three joint inspection according to claim 1, which is characterized in that the sample cell (2) is funnel-form, described
First filter layer (9) is strainer, and the second filter layer (10) is filter membrane.
5. card is surveyed in a kind of three joint inspection according to claim 1, which is characterized in that the observation window (3) is separately positioned on phase
Above the chromatographic film (13) answered, and corresponding number (6) is equipped with beside each observation window (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821581978.1U CN208833770U (en) | 2018-09-27 | 2018-09-27 | A kind of three joint inspections survey card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821581978.1U CN208833770U (en) | 2018-09-27 | 2018-09-27 | A kind of three joint inspections survey card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208833770U true CN208833770U (en) | 2019-05-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821581978.1U Active CN208833770U (en) | 2018-09-27 | 2018-09-27 | A kind of three joint inspections survey card |
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| Country | Link |
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| CN (1) | CN208833770U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116087500A (en) * | 2022-12-26 | 2023-05-09 | 科赫生物科技（北京）有限公司 | Multi-joint detection device and application method thereof |
-
2018
- 2018-09-27 CN CN201821581978.1U patent/CN208833770U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116087500A (en) * | 2022-12-26 | 2023-05-09 | 科赫生物科技（北京）有限公司 | Multi-joint detection device and application method thereof |
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