CN214571895U - Cell suspension culture device - Google Patents
Cell suspension culture device Download PDFInfo
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- CN214571895U CN214571895U CN202120392527.9U CN202120392527U CN214571895U CN 214571895 U CN214571895 U CN 214571895U CN 202120392527 U CN202120392527 U CN 202120392527U CN 214571895 U CN214571895 U CN 214571895U
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- biological reaction
- pipe
- nutrient solution
- injection tube
- solution injection
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Abstract
The utility model discloses a cell suspension culture apparatus, biological reaction jar's top right side is equipped with the breather pipe, biological reaction jar's top middle part is equipped with the stoste pipe, the left side of stoste pipe is equipped with ozone disinfection injection tube an, biological reaction jar's upper left side is equipped with nutrient solution injection tube an, biological reaction jar's upper right side is equipped with nutrient solution injection tube b, nutrient solution injection tube b's top and nutrient solution injection tube an's top all are equipped with ozone disinfection injection tube b, biological reaction jar's right side below is equipped with the waste liquid outlet pipe, the inboard of waste liquid outlet pipe has clamped the micro-filtration membrane, the top of waste liquid outlet pipe, ozone disinfection injection tube b's side, nutrient solution injection tube b's top, the valve is all installed to nutrient solution injection tube an's top and ozone disinfection injection tube an's top. Can reduce the damage to the cell to through the setting of ozone disinfection injection tube, can disinfect the thing of treating that injects and nutrient solution, thereby guarantee culture apparatus's normal culture.
Description
Technical Field
The utility model relates to a culture apparatus field, concretely relates to cell suspension culture apparatus.
Background
The suspension culture of plant cells means that plant cells or small cell masses are cultured in a liquid medium in a suspended state, and a well-dispersed state can be maintained during the culture. The callus can be generated by in vitro culture of the plant. After the loose type callus county floats in the liquid medium and is cultured under shaking conditions for a certain period of time, a scatter county float culture can be formed. When carrying out cell suspension culture, need use cell suspension culture device usually, current common cell suspension culture device mainly adopts the mode of mixing earlier the postculture, and the stirring leaf is mostly harder, at rotatory in-process, harms the cell easily to in case mix the bacterium in the culture or the nutrient solution of treating that inject, will influence culture device's normal culture.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned drawbacks or deficiencies of the prior art, it would be desirable to provide a cell suspension culture device that reduces damage to cells and also sterilizes injected culture and nutrient solutions.
According to the technical scheme provided by the embodiment of the utility model, a cell suspension culture device, including biological reaction jar, biological reaction jar's top right side is equipped with the breather pipe, biological reaction jar's top middle part is equipped with the stoste pipe, stoste pipe's left side is equipped with ozone disinfection injection tube an, biological reaction jar's upper left side is equipped with nutrient solution injection tube a, biological reaction jar's upper right side is equipped with nutrient solution injection tube b, nutrient solution injection tube b's top and nutrient solution injection tube an's top all are equipped with ozone disinfection injection tube b, biological reaction jar's right side below is equipped with the waste liquid outlet pipe, the inboard of waste liquid outlet pipe clamps the micro-filtration membrane, the valve is all installed to the top of waste liquid outlet pipe, the side of ozone disinfection injection tube b, the top of nutrient solution injection tube an and the top of ozone disinfection injection tube a, the biological reaction tank is clamped above the fixing seat, the fixing seat comprises a limiting block and supporting legs, the supporting legs are welded on the left side and the right side below the limiting block, the limiting block comprises a flat plate and a limiting ring, the limiting ring is welded on the flat plate, a protective pad is clamped on the inner side of the limiting ring, a machine base is welded on the lower side of the flat plate, an electric motor is installed below the machine base through screws, an output shaft is arranged above the electric motor, a stirring shaft is connected above the output shaft through a coupler, the top of the stirring shaft penetrates through the bottom of the biological reaction tank and extends into the inner side of the biological reaction tank, through holes a corresponding to the stirring shaft are formed in the bottom surface of the protective pad and the surface of the flat plate, the bottom of the biological reaction tank is a tank bottom, and a rotary sealing ring is assembled on the surface of the tank bottom and positioned outside the stirring shaft, the stirring shaft is characterized in that a stirring blade is welded on the outer side of the stirring shaft, a positioning ring is arranged in the middle of the stirring blade, blades are arranged on the left side and the right side of the stirring blade, and a flexible sleeve is sleeved on the outer side of each blade.
The utility model discloses in, the biological reaction jar is the toughened glass material, the top left and right sides of biological reaction jar is the arc structure.
The utility model discloses in, the breather pipe the stoste pipe ozone disinfection filling tube an nutrient solution filling tube a nutrient solution filling tube b ozone disinfection filling tube b the waste liquid outlet pipe with as an organic whole becomes the formula structure between the biological reaction jar.
The utility model discloses in, nutrient solution filling tube b highly be less than the height of breather pipe, the diameter of breather pipe is less than nutrient solution filling tube b's diameter.
The utility model discloses in, the fixing base is the metal material, the protection is filled up and is located biological reaction jar with between the spacing ring.
The utility model discloses in, the bottom surface of frame be equipped with the corresponding through-hole b of output shaft, electric motor is located the left and right sides between the supporting leg.
The utility model discloses in, the flexible cover is the silica gel material, the stirring leaf is located biological reaction tank's inboard, the protection pad is the rubber material.
To sum up, the utility model has the advantages that: through the setting of flexible cover, can reduce the damage to the cell to through the setting of ozone disinfection injection tube, can disinfect the injected culture of treating and nutrient solution, thereby guarantee culture apparatus's normal culture.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments made with reference to the following drawings:
fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top view of the connection between the fixing base and the protection pad of the present invention;
FIG. 3 is a schematic diagram of a side view of the microfiltration membrane of the present invention;
fig. 4 is a schematic view of the three-dimensional structure of the protection pad of the present invention.
Reference numbers in the figures: 1. a biological reaction tank; 2. a breather pipe; 3. a stock solution conduit; 4. ozone disinfecting injection tube a; 5. a nutrient solution injection pipe a; 6. a nutrient solution injection pipe b; 7. ozone disinfection injection pipe b; 8. a waste liquid outflow pipe; 9. a microfiltration membrane; 10. a valve; 11. a fixed seat; 12. a protective pad; 13. a machine base; 14. an electric motor; 15. a stirring shaft; 16. stirring blades; 17. a flexible sleeve.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the relevant invention and not restrictive of the invention. It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings.
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
Referring to fig. 1, 2, 3 and 4, a cell suspension culture apparatus includes a bioreactor 1, a vent pipe 2 is disposed on the right side of the upper portion of the bioreactor 1, a raw liquid guide pipe 3 is disposed in the middle of the upper portion of the bioreactor 1, an ozone disinfection injection pipe a4 is disposed on the left side of the raw liquid guide pipe 3, a nutrient solution injection pipe a5 is disposed on the left upper portion of the bioreactor 1, a nutrient solution injection pipe b6 is disposed on the right upper portion of the bioreactor 1, ozone disinfection injection pipes b7 are disposed above the nutrient solution injection pipe b6 and above the nutrient solution injection pipe a5, a waste liquid outflow pipe 8 is disposed on the right lower portion of the bioreactor 1, a microfiltration membrane 9 is clamped inside the waste liquid outflow pipe 8, a side surface of the ozone disinfection injection pipe b7 and above the nutrient solution injection pipe b6, The valve 10 is installed above the nutrient solution injection pipe a5 and above the ozone disinfection injection pipe a4, the biological reaction tank 1 is clamped above the fixed seat 11, the fixed seat 11 comprises a limiting block and supporting legs, the supporting legs are welded at the left side and the right side of the lower side of the limiting block, the limiting block comprises a flat plate and a limiting ring, the limiting ring is welded above the flat plate, a protective pad 12 is clamped at the inner side of the limiting ring, a machine seat 13 is welded below the flat plate, an electric motor 14 is installed below the machine seat 13 through screws, the model of the electric motor 14 is 5IK120GN-C, an output shaft is arranged above the electric motor 14, a stirring shaft 15 is connected above the output shaft through a coupler, the top of the stirring shaft 15 penetrates through the bottom of the biological reaction tank 1 and extends into the inner side of the biological reaction tank 1, the bottom surface of protection pad 12 with dull and stereotyped surface all be equipped with the corresponding through-hole a of (mixing) shaft 15, the bottom of biological reaction jar 1 is the tank bottoms, the surface of tank bottoms just is located the outside of (mixing) shaft 15 is equipped with rotary seal circle, the outside welding of (mixing) shaft 15 has stirring leaf 16, stirring leaf 16's middle part is the holding ring, stirring leaf 16's the left and right sides is the blade, the outside cover of blade is equipped with flexible cover 17.
As shown in figure 1, the biological reaction tank 1 is made of toughened glass, and the left side and the right side above the biological reaction tank 1 are of arc structures. The aeration pipe 2, the raw liquid guide pipe 3, the ozone disinfection injection pipe a4, the nutrient solution injection pipe a5, the nutrient solution injection pipe b6, the ozone disinfection injection pipe b7, the waste liquid outflow pipe 8 and the biological reaction tank 1 are integrally formed.
As shown in fig. 1, 2 and 4, the height of the nutrient solution injection pipe b6 is lower than that of the aeration pipe 2, and the diameter of the aeration pipe 2 is smaller than that of the nutrient solution injection pipe b 6. The fixing seat 11 is made of metal, and the protection pad 12 is located between the biological reaction tank 1 and the limiting ring.
As shown in fig. 1, 2 and 4, a through hole b corresponding to the output shaft is formed in the bottom surface of the base 13, and the electric motor 14 is located between the left and right support legs. Flexible cover 17 is the silica gel material, stirring leaf 16 is located the inboard of biological reaction jar 1, protection pad 12 is the rubber material, and protection pad 12 can protect 1 tank bottoms of biological reaction jar to avoid wearing and tearing.
Example 1: the aeration pipe 2 is arranged at the right side above the biological reaction tank 1 and is of an integrated structure, the stock solution guide pipe 3 is arranged at the middle part above the biological reaction tank 1 and is of an integrated structure, the ozone disinfection injection pipe a4 is arranged at the left side of the stock solution guide pipe 3 and is of an integrated structure, the nutrient solution injection pipe a5 is arranged at the upper left side of the biological reaction tank 1 and is of an integrated structure, the nutrient solution injection pipe b6 is arranged at the upper right side of the biological reaction tank 1 and is of an integrated structure, the ozone disinfection injection pipe b7 is respectively arranged above the nutrient solution injection pipe b6 and above the nutrient solution injection pipe a5 and is of an integrated structure, the waste liquid outflow pipe 8 is arranged at the lower right side of the biological reaction tank 1 and is of an integrated structure, the microfiltration membrane 9 is clamped at the inner side of the waste liquid outflow pipe 8, the valve 10 is respectively installed above the waste liquid outflow pipe 8, the side surface of the ozone disinfection injection pipe b7, the upper side of the nutrient solution injection pipe b6, the upper side of the nutrient solution injection pipe a5 and the upper side of the ozone disinfection injection pipe a4, the biological reaction tank 1 is clamped above the fixed seat 11, the protective pad 12 is clamped inside the limit ring, the base 13 is welded below the flat plate, the electric motor 14 is installed below the base 13 through screws, the stirring shaft 15 is connected above the output shaft through a coupling, the stirring blade 16 is welded outside the stirring shaft 15, and the flexible sleeve 17 is sleeved outside the blade.
Example 2: firstly, a to-be-cultured substance is injected into a biological reaction tank 1 through a stock solution conduit 3, meanwhile, ozone is injected into the stock solution conduit 3 through an ozone disinfection injection pipe a4, the to-be-cultured substance can be disinfected, then, nutrient solution is injected into the biological reaction tank 1 through a nutrient solution injection pipe a5 and a nutrient solution injection pipe b6, meanwhile, ozone is injected into a nutrient solution injection pipe a5 and a nutrient solution injection pipe b6 through an ozone disinfection injection pipe b7, the ozone can disinfect the nutrient solution, so that the biological reaction tank 1 is in a sterile environment, a culture device can be ensured to be normally cultured, then, an electric motor 14 is started, a stirring shaft 15 can drive a stirring blade 16 to rotate, objects in the biological reaction tank 1 are uniformly mixed, because the outer side of the stirring blade 16 is sleeved with a flexible sleeve 17, damage to cells can be reduced, when the nutrient solution needs to be replaced, a right valve 10 can be opened below the biological reaction tank, the waste liquid will flow out through the waste liquid outflow pipe 8, and in the process of culturing, sterile air can be injected into the biological reaction tank 1 through the vent pipe 2.
The foregoing description is only exemplary of the preferred embodiments of the invention and is provided for the purpose of illustrating the general principles of the invention. Meanwhile, the scope of the present invention is not limited to the specific combinations of the above-described technical features, and other technical features or equivalent features may be combined arbitrarily without departing from the scope of the present invention. For example, the above features and (but not limited to) technical features having similar functions disclosed in the present invention are mutually replaced to form the technical solution.
Claims (7)
1. A cell suspension culture device comprises a biological reaction tank (1), and is characterized in that: the top right side of biological reaction jar (1) is equipped with breather pipe (2), the top middle part of biological reaction jar (1) is equipped with stoste pipe (3), the left side of stoste pipe (3) is equipped with ozone disinfection injection tube a (4), the upper left side of biological reaction jar (1) is equipped with nutrient solution injection tube an (5), the upper right side of biological reaction jar (1) is equipped with nutrient solution injection tube b (6), the top of nutrient solution injection tube b (6) with the top of nutrient solution injection tube a (5) all is equipped with ozone disinfection injection tube b (7), the right side below of biological reaction jar (1) is equipped with waste liquid outlet pipe (8), the inboard of waste liquid outlet pipe (8) clamps microfiltration membrane (9), the top of waste liquid outlet pipe (8), the side of ozone disinfection injection tube b (7), the top of nutrient solution injection tube b (6), The upper part of the nutrient solution injection pipe a (5) and the upper part of the ozone disinfection injection pipe a (4) are both provided with a valve (10), the biological reaction tank (1) is clamped above a fixing seat (11), the fixing seat (11) comprises a limiting block and supporting legs, the supporting legs are welded at the left side and the right side below the limiting block, the limiting block comprises a flat plate and a limiting ring, the limiting ring is welded above the flat plate, the inner side of the limiting ring is clamped with a protective pad (12), a machine base (13) is welded below the flat plate, an electric motor (14) is installed below the machine base (13) through screws, an output shaft is arranged above the electric motor (14), the upper part of the output shaft is connected with a stirring shaft (15) through a coupler, the top of the stirring shaft (15) penetrates through the bottom of the biological reaction tank (1) and extends into the inner side of the biological reaction tank (1), the bottom surface of protection pad (12) with dull and stereotyped surface all be equipped with the corresponding through-hole a of (mixing) shaft (15), the bottom of biological reaction jar (1) is the tank bottoms, the surface of tank bottoms just is located the outside of (mixing) shaft (15) is equipped with rotary seal, the outside welding of (mixing) shaft (15) has stirring leaf (16), the middle part of stirring leaf (16) is the holding ring, the left and right sides of stirring leaf (16) is the blade, the outside cover of blade is equipped with flexible cover (17).
2. A cell suspension culture apparatus according to claim 1, wherein: the biological reaction tank (1) is made of toughened glass, and the left side and the right side of the upper part of the biological reaction tank (1) are of arc structures.
3. A cell suspension culture apparatus according to claim 1, wherein: the aeration pipe (2), the stock solution guide pipe (3), the ozone disinfection injection pipe a (4), the nutrient solution injection pipe a (5), the nutrient solution injection pipe b (6), the ozone disinfection injection pipe b (7), the waste liquid outflow pipe (8) and between the biological reaction jar (1) as an organic whole formula structure.
4. A cell suspension culture apparatus according to claim 1, wherein: the height of the nutrient solution injection pipe b (6) is lower than that of the vent pipe (2), and the diameter of the vent pipe (2) is smaller than that of the nutrient solution injection pipe b (6).
5. A cell suspension culture apparatus according to claim 1, wherein: the fixing seat (11) is made of metal, and the protective pad (12) is located between the biological reaction tank (1) and the limiting ring.
6. A cell suspension culture apparatus according to claim 1, wherein: the bottom surface of frame (13) is equipped with the through-hole b that the output shaft corresponds, electric motor (14) are located the left and right sides between the supporting leg.
7. A cell suspension culture apparatus according to claim 1, wherein: the flexible sleeve (17) is made of silica gel, the stirring blade (16) is located on the inner side of the biological reaction tank (1), and the protective pad (12) is made of rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120392527.9U CN214571895U (en) | 2021-02-22 | 2021-02-22 | Cell suspension culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120392527.9U CN214571895U (en) | 2021-02-22 | 2021-02-22 | Cell suspension culture device |
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| CN214571895U true CN214571895U (en) | 2021-11-02 |
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| CN202120392527.9U Active CN214571895U (en) | 2021-02-22 | 2021-02-22 | Cell suspension culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117947138A (en) * | 2024-03-27 | 2024-04-30 | 佳木斯大学 | In-vitro cytotoxicity test method for oral cavity material |
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2021
- 2021-02-22 CN CN202120392527.9U patent/CN214571895U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117947138A (en) * | 2024-03-27 | 2024-04-30 | 佳木斯大学 | In-vitro cytotoxicity test method for oral cavity material |
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