CN214496356U - Cell culture bottle - Google Patents

Abstract

The utility model relates to the field of cell culture utensils, in particular to a cell culture bottle. The utility model discloses a bottle, bottle lid and gasket, the bottleneck slope is upwards, the bottle lid has air vent and screw-thread engagement the bottleneck, the gasket evenly distributed on the bottle lateral wall the gasket, it is a plurality of the gasket paves in the bottle, the gasket has the shrinkage pool, and the width is less than the bottleneck diameter. The utility model discloses a cell is difficult for the caking when growing, and the cytostatic phenomenon reduces by a wide margin, and it reaches 80% to judge the cell fusion degree according to the cell is full of shrinkage pool edge, but timely interval change cell gasket in order to carry out the cell passage, omits the step of pancreatin digestion, simplifies the procedure of cell passage, reduces the purpose of frequently observing the work load of cell fusion degree through the microscope.

Description

Cell culture bottle

Technical Field

The utility model relates to the field of cell culture utensils, in particular to a cell culture bottle.

Background

Cells are the basic structural and functional units of all vital activities. With the rapid development of biotechnology, cell culture technology has become an indispensable technical means in scientific research or production. Most of the cells are grown by adherent growth at present, the cells are attached to the bottom of a culture bottle, mitosis starts after a period of time and rapidly enter the logarithmic phase of the cells, and the cells usually grow for several days and then cover the bottom of the culture bottle. When the cells contact with each other to form a sheet, because glycoprotein exists on cell membranes, the cell sheets can perform mutual recognition, and then enter a density inhibition state, cell division is gradually stopped, and with the gradual consumption of nutrient components in a culture solution, metabolites are increased, and finally, the cells completely stop dividing and die. Therefore, in the conventional cell culture technology, when the cell fusion degree reaches about 80%, the cells need to be passaged to increase the growth space of the cells. The current cell culture is generally carried out by using culture dishes or culture flasks, and the cell generation needs to follow standard generation steps, which is time-consuming and labor-consuming.

CN206157173U has disclosed a cell culture bottle for adherent cell growth, at the growth lateral wall evenly distributed cell growth shrinkage pool of body cell, through this kind of cell culture bottle with the design of hemisphere shrinkage pool, can be according to the position in hole and fill up the size in every hole when with artifical partial shipment adherent cell tissue, adherent cell tissue distributes evenly like this, and its position and quantity can all be controlled. However, when the cells are passaged in the cell culture bottle, the narrow bottle mouth makes the cell passage operation in the concave hole very inconvenient.

SUMMERY OF THE UTILITY MODEL

To the problem, the utility model provides a cell culture bottle has the gasket of removable cell growth in the bottle, realizes that cell passage easy operation, avoids the cell to conglomerate, monitors the easy purpose of cell fusion degree.

The utility model provides a technical scheme as follows:

a cell culture bottle comprises a bottle body, a bottle cap and a plurality of gaskets, wherein the bottle body is transversely placed and tilted, a bottle opening is tilted upwards so as to be clamped and put in the gaskets, the bottle cap with a vent hole is meshed with the bottle opening through threads, and ventilation is realized while external environment pollution is prevented; the gasket is transversely and evenly tiled in the bottle body, the gasket is provided with a concave hole, and the width of the gasket is smaller than the diameter of the bottle opening, so that the gasket can be conveniently taken out of the bottle opening for replacement.

Further, the width of the gasket is 1.5cm-2.0cm, the thickness of the gasket is 5mm, and the length of the gasket is consistent with the inner space of the bottle body.

Furthermore, the concave hole is cylindrical, the transverse cross section of the concave hole is circular, the longitudinal cross section of the concave hole is rectangular, the depth of the concave hole is 0.8-1.2mm, and cells can be limited to grow only around the concave hole, so that contact among partial cells is avoided, the cell inhibition phenomenon is reduced, and the cells are prevented from stopping growing and dying.

Furthermore, the total area of the concave holes is about 20% of the area of the gasket, so that the contact area of the cell culture solution and the cells at the bottom of the bottle is always larger than 80%, frequent cell counting can be avoided, and the next generation of passage operation can be carried out only by observing whether the cells are full, and the full growth indicates that the fusion degree reaches 80%, and the problem of nutrient component deficiency can not occur very quickly.

Further, the gasket is a rectangular gasket made of medical polystyrene material with high transparency.

Furthermore, the diameter of the bottle mouth is larger than the width of the gasket, the diameter of the bottle mouth is 2.5cm-3.0cm, the gasket can be taken out easily, and the method is more convenient and faster during cell passage.

Further, the width of the inner space of the bottle body is 4-6 times of the width of the growth gasket.

The beneficial effect that adopts this technical scheme to reach does:

1. by designing the replaceable gasket in the culture bottle, when the cell fusion degree reaches about 80%, the cell gasket can be replaced at intervals in time for cell passage, a new gasket without cells is put in to increase the growth space of the cells, and then culture is continued, so that the traditional passage steps can be reduced, and the efficiency of continuously obtaining cell supernatant for extracting exosomes is increased.

2. Through set up the shrinkage pool on the gasket for cylindrical, the cell grows on the gasket surface, has not only increased the area that the cell grows, and the cell does not grow on perpendicular pore wall, has still avoided intercellular suppression phenomenon, keeps the cell activity, also makes the cell be difficult for the conglomeration when growing.

3. By setting the area of the concave hole to be about 20% of the area of the gasket, when the gasket surface is observed to be full of cells, the fusion degree of the cells reaches 80%, the growth gasket is directly replaced, and a new gasket without the cells is placed, so that the growth space of the cells is increased, namely the cell passage is completed, the step of pancreatin digestion is omitted, the procedure of the cell passage is simplified, and the workload of frequently observing the fusion degree of the cells through a microscope is reduced.

Drawings

Fig. 1 is the schematic plan structure of the present invention:

fig. 2 is a schematic structural view of the utility model:

1 bottle body, 2 gaskets, 3 shrinkage holes, 4 bottle openings, 11 gaskets I, 12 gaskets II, 13 gaskets III and 14 gaskets IV.

Detailed Description

For a better understanding of the technical solutions of the present technology, the present technology is described in detail below with reference to the accompanying drawings, and the description of the present technology is only exemplary and explanatory, and should not be construed as limiting the scope of the present technology in any way.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the art are used, and are used only for convenience in describing the technology and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the technology.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present technology, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present technology can be understood in a specific case to those of ordinary skill in the art.

As shown in fig. 1-2, the utility model relates to a cell culture bottle, including bottle 1, bottle lid and four gaskets 2, bottleneck 4 slopes to the top just press from both sides to get and put into gasket 2, has the air vent bottle lid thread engagement bottleneck 4 ventilates when preventing external environment pollution, horizontal even tiling gasket 2 in the bottle 1, gasket 2 have shrinkage pool 3, and the cell growth is in the position of 2 surperficial non-shrinkage pools 3 of gasket, carries out the mitosis on gasket 2. The concave hole 3 on the gasket 2 makes the cells not easy to agglomerate when growing, so that the step of trypsinization can be omitted when the cells are passaged, and the procedure of cell passaging is simplified.

When the gasket 2 is full of cells, the gasket I11 and the gasket III 13 are extracted and replaced by a new gasket 2, the gasket I11 and the gasket III 13 are placed in another culture bottle, and the new gasket 2 is placed at intervals, so that the cells at the edge of the gasket 2 obtain a growth space to continue to grow for cell passage.

Preferably, the width of the gasket 2 is 1.5cm, the thickness is 5mm, and the length is consistent with the length of the inner space of the bottle body 1.

Preferably, in order to facilitate the growth of cells, the concave hole 3 is cylindrical, the transverse cross section is circular, the longitudinal cross section is rectangular, and the depth is 1mm, so that the cells can be limited not to grow on the vertical wall but only grow around the vertical wall, thereby avoiding the contact between partial cells, reducing the cell inhibition phenomenon, and avoiding the stop of the growth and death of the cells.

Preferably, the total area of the concave holes 3 is about 20% of the area of the gasket 2, so that the contact area of the cell culture solution and the cells at the bottom of the bottle is always larger than 80%, the problem of nutrient content cannot occur quickly, and the observation of whether the cell fusion degree reaches 80% or not is facilitated, and cell passage is required.

Preferably, the gasket 2 is a rectangular gasket made of medical polystyrene material with high transparency.

Preferably, the diameter of the bottle mouth 4 is slightly wider than the width of the gasket 2, the diameter is 2.5-3cm, the gasket 2 can be easily taken out, and the convenience are realized during cell passage.

Preferably, the width of the inner space of the bottle body 1 is 4 times of the width of the gasket 2, so that more cells can be cultured in one culture bottle in a reasonable range.

The utility model discloses the principle is: by designing the replaceable gasket 2 in the culture bottle, the gasket 2 is provided with the irregularly distributed cylindrical concave holes 3, and the sum of the areas of the concave holes 3 is about 20 percent of the area of the gasket, so when cells are cultured, the cells grow on the surface of the gasket 2 and cannot grow on the vertical wall of the concave holes 2, and the concave holes 3 reduce the contact among the cells, namely reduce the phenomenon of cell inhibitory factors; when the surface of the pad 2 is observed to be full of cells, namely the cell fusion degree is about 80%, the pad 2 can be replaced at intervals in time for cell passage (for example, the pad I11 and the pad III 13 are extracted and replaced by a new pad 2, the pad I11 and the pad III 13 are placed in another culture bottle, and the new pad 2 is placed at intervals), so that the cells at the edge of the pad 2 obtain a growth space for continuous growth, and the step of trypsinization is omitted for cell passage.

It should be noted that there are no specific structures in the above description, and it will be apparent to those skilled in the art that various modifications, decorations, or changes can be made without departing from the technical principles of the present invention; such modifications, variations, or combinations, or applying the concepts and solutions of the technology directly to other applications without further modifications, are intended to be within the scope of the present technology.

Claims (7)

1. A cell culture flask, characterized in that: including bottle, bottle lid and a plurality of gasket, the bottleneck slope is upwards, the bottle lid has air vent and screw-thread engagement the bottleneck, the gasket evenly distributed on the bottle lateral wall the gasket is a plurality of the gasket lay in the bottle, the gasket width is less than the bottleneck diameter, just the gasket has the shrinkage pool.

2. The cell culture flask according to claim 1, wherein: the width of the gasket is 1.5cm-2.0cm, the thickness is 3-8mm, and the length is consistent with the length of the inner space of the bottle body.

3. The cell culture flask according to claim 2, wherein: the concave hole is cylindrical, the transverse cross section of the concave hole is circular, the longitudinal cross section of the concave hole is rectangular, and the depth of the concave hole is 0.8-1.2 mm.

4. The cell culture flask according to claim 2, wherein: the sum of the areas of the concave holes is 20% -30% of the area of the gasket.

5. The cell culture flask according to claim 2, wherein: the gasket is rectangular and made of medical polystyrene with high transparency.

6. The cell culture flask according to claim 1, wherein: the diameter of the bottle mouth is 2.5cm-3.0 cm.

7. The cell culture flask according to claim 1, wherein: the width of the inner space of the bottle body is 4-6 times of the width of the gasket.

Images