CN214496350U

CN214496350U - Culture apparatus that placenta matrix mesenchymal stem cell used

Info

Publication number: CN214496350U
Application number: CN202120125091.7U
Authority: CN
Inventors: 任鹏; 陈淑倩; 魏瑞冬; 武晓东; 张艳茹; 苏圆; 刘晓玲; 吕联军; 李建强; 王素田
Original assignee: Shanxi Stem Cell Genetic Engineering Co ltd
Current assignee: Shanxi Stem Cell Genetic Engineering Co ltd
Priority date: 2021-01-18
Filing date: 2021-01-18
Publication date: 2021-10-26
Anticipated expiration: 2031-01-18

Abstract

The utility model discloses a culture apparatus that placenta matrix mesenchymal stem cell used, including first cultivation room, spacing seat, second cultivation room and casing, spacing seat is installed to the bottom of casing, and the internally mounted of spacing seat has the vacuum pump to the bottom mounting of spacing seat has the air guide plate. The utility model discloses an install the aspiration pump, the exhaust tube, a housing, controller and vacuum sensor, make the device optimize the performance of self, during the use, start the aspiration pump, effect through the exhaust tube, take the inside gas of casing out, meanwhile, vacuum sensor can monitor the inside gaseous condition of casing constantly, and data transmission to monitoring gives the controller, when the inside vacuum state that is in of device, controller control aspiration pump closes, and the solenoid valve in the control intake pipe is opened, with gaseous inside through the leading-in casing of air guide equipment will be cultivateed, and then make the device can regulate and control the inside gaseous environment of casing.

Description

Culture apparatus that placenta matrix mesenchymal stem cell used

Technical Field

The utility model relates to a cell culture technical field specifically is a culture apparatus that placenta matrix mesenchymal stem cell used.

Background

Placenta matrix mesenchymal stem cell has powerful proliferation ability and multidirectional differentiation potential, compares in other stem cells, and it generally possesses advantages such as source convenience, cell quantity is sufficient, easily separation, cultivation, amplification and purification, consequently often adopts placenta matrix mesenchymal stem cell as the experimental object in the laboratory, and just need use a culture apparatus when cultivateing placenta matrix mesenchymal stem cell, but the culture apparatus that present placenta matrix mesenchymal stem cell used still has following several problems when in-service use:

1. the common culture device for the placenta maternal mesenchymal stem cells has the defects of single structure and difficulty in realizing classified storage of cell samples and contrast experiments of different cell samples, so that the use effect of the device is poor;

2. the existing culture device for the placenta maternal mesenchymal stem cells on the market also has the defects of poor regulation and control effect of culture environment and difficulty in realizing dual regulation and control of gas and temperature, so that the device has poor functionality and is not beneficial to long-term popularization;

3. the existing culture device for the placenta maternal mesenchymal stem cells generally has the problems of poor stability and difficult disassembly and assembly maintenance, which leads to low practicability of the device.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture apparatus that placenta matrix mesenchymal stem cell used to solve the single structure that proposes in the above-mentioned background art, be difficult for realizing that the classification of cell sample is deposited and the contrast experiment of different cell samples, culture environment regulation and control effect is not good, be difficult for realizing the problem of maintaining to the dual regulation and control and the poor stability of gas and temperature and difficult dismouting.

In order to achieve the above object, the utility model provides a following technical scheme: a culture apparatus for placenta matrix mesenchymal stem cells comprises a first culture chamber, a limiting seat, a second culture chamber and a casing, wherein the bottom end of the casing is provided with the limiting seat, the interior of the limiting seat is provided with a vacuum pump, the bottom end of the limiting seat is fixedly provided with an air guide plate, one end of the casing is provided with a controller, the top end of the casing is provided with an air pump, the input end of the air pump is communicated with the top end of the interior of the casing through an air exhaust pipe, one end of the interior of the casing is provided with the first culture chamber, the other end of the interior of the casing is provided with the second culture chamber, heating wires are laid in the side wall of the casing, the interiors of the first culture chamber and the second culture chamber are respectively provided with a storage rack, the top end of the interior of the casing is sequentially provided with a temperature sensor and a vacuum sensor, one side of the interior of the casing is fixedly provided with an air inlet pipe, and the air inlet pipe is provided with an electromagnetic valve, and one side of the air inlet pipe, which is far away from the shell, is provided with a flange plate.

Preferably, the interior of the first culture chamber is communicated with the interior of the second culture chamber, and the first culture chamber and the second culture chamber are symmetrically distributed about the vertical center line of the machine shell, so that the structure of the device is optimized, and the stability of the whole structure of the device is improved through symmetrical distribution.

Preferably, the supporter is provided with 2, and all welds at the both ends of supporter and have reserved the draw runner, and the lateral wall of reserving the draw runner sets up anti-skidding rubber layer, and first culture room and second culture room inside both ends all are provided with and reserve the spout with reserving draw runner assorted reservation, make its convenient to use person set up two sets of placenta matrix mesenchymal stem cells and carry out the contrast and cultivate.

Preferably, the inside of supporter all evenly is fixed with the partition groove, and the partition groove is hollow structure, and the inside wall that just divides the partition groove is provided with the silica gel inoxidizing coating, is provided with sealing door on the casing for the cultivation of a plurality of samples can be realized to the device, has strengthened the practicality of device.

Preferably, four corners of air guide plate bottom all are fixed with the adsorption disc, and the bottom of adsorption disc evenly is provided with the absorption hole, and the inside of adsorption disc and the inside of air guide plate are linked together, and constitute integrated welded structure between air guide plate and the spacing seat, and the input of vacuum pump is linked together through connecting pipe and the inside top of air guide plate, makes it realize fixed spacing effect to the device, has strengthened the stationarity ability of device.

Preferably, the connection cutting is welded at both ends of the bottom of the machine shell, the top of the limiting seat is uniformly provided with a connection slot matched with the connection cutting, one side of the connection cutting is welded with a locking piece, and the locking piece is provided with a locking bolt connected with the limiting seat, so that the device has the advantages of realizing the combination and is convenient to clean and maintain.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) the culture device for the placenta matrix mesenchymal stem cells optimizes the structure of the device by installing the storage rack, the separation groove, the first culture chamber and the second culture chamber, when in use, on one hand, the first culture chamber and the second culture chamber are arranged on the device, and the storage rack is arranged in each culture chamber, so that a user can conveniently arrange two groups of placenta matrix mesenchymal stem cells for contrast culture, the functionality of the device is enhanced, on the other hand, the user can disassemble or assemble the storage rack in the first culture chamber and the second culture chamber by utilizing a sliding connection structure formed by the reserved sliding strips and the reserved sliding grooves, so that the cultured storage rack can be conveniently taken out, a new storage rack to be cultured for the placenta matrix mesenchymal stem cells is replaced, the convenience of the operation of the device is enhanced, and the separation grooves are uniformly fixed in the storage rack, a plurality of experimental samples are conveniently arranged on each group of placenta matrix mesenchymal stem cells, and each culture tube filled with the experimental samples and culture solution is sequentially clamped into each separation groove for classified storage, so that the device can realize the culture of a plurality of samples, and the practicability of the device is enhanced;

(2) when the device is used, on one hand, the air pump is started to pump out air in the shell under the action of the air pump, meanwhile, the vacuum sensor can constantly monitor the air condition in the shell and send the monitored data to the controller, when the device is in a vacuum state, the controller controls the air pump to be closed and controls the electromagnetic valve on the air inlet pipe to be opened, the culture air is introduced into the shell through the air guide equipment, so that the device can regulate and control the air environment in the shell to be beneficial to the growth of the placenta maternal mesenchymal stem cells, on the other hand, the heating wire is controlled to be electrified to generate heat in the shell, meanwhile, the temperature sensor can constantly monitor the gas condition in the casing and send the monitored data to the controller, when the temperature reaches a preset value, the controller controls the heating wire to be powered off, so that the device can accurately regulate and control the internal environment temperature, and a proper survival temperature is conveniently created for mesenchymal stem cells of the placenta matrix, so that the device can realize regulation on the gas environment and the temperature environment, and the functionality of the device is enhanced;

(3) the placenta matrix mesenchymal stem cell culture device is provided with the locking sheet, the adsorption disc, the air guide plate, the locking bolt, the connecting inserting strip and the connecting slot, so that when the device is used specifically, on one hand, after a user carries the device to a proper operation table board, the vacuum pump can be started, the inside of the air guide plate is vacuumized under the action of the connecting pipe, and then the inside of the adsorption disc communicated with the inside of the air guide plate is vacuumized, negative pressure is generated inside an adsorption hole at the bottom end of the adsorption disc, the corresponding operation table board is firmly adsorbed, the fixing and limiting effects on the device can be realized, the stability of the device is enhanced, on the other hand, the user can utilize an inserting and connecting structure formed by the connecting inserting strip and the connecting slot to cooperate with the locking and fixing effects of the locking sheet and the locking bolt to disassemble or assemble the machine shell and the limiting seat, and further the device realizes the combined advantages, is convenient for cleaning and maintenance.

Drawings

Fig. 1 is a schematic view of a front cross-sectional structure of a culture apparatus for placental maternal mesenchymal stem cells according to the present invention;

fig. 2 is a schematic side view of a casing of a culture device for placental mesenchymal stem cells according to the present invention;

fig. 3 is an enlarged schematic structural view of a portion a in fig. 1 of a culture apparatus for placental maternal mesenchymal stem cells according to the present invention;

figure 4 is the utility model discloses a culture apparatus intake pipe side view section structure sketch map that placenta matrix mesenchymal stem cell used.

In the figure: 1. heating wires; 2. a first culture chamber; 3. a rack; 301. a separation tank; 4. reserving a chute; 5. an adsorption tray; 6. a gas guide plate; 7. a vacuum pump; 8. a limiting seat; 9. reserving a slide bar; 10. a second culture chamber; 11. a housing; 12. a temperature sensor; 13. an air pump; 14. an air inlet pipe; 15. a vacuum sensor; 16. connecting the inserting strips; 17. an air exhaust pipe; 18. a controller; 19. connecting the slots; 20. a locking piece; 21. locking the bolt; 22. an electromagnetic valve; 23. a flange plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides an embodiment: a culture device for placenta matrix mesenchymal stem cells comprises a first culture chamber 2, a limiting seat 8, a second culture chamber 10 and a casing 11, wherein the limiting seat 8 is installed at the bottom end of the casing 11, a vacuum pump 7 is installed inside the limiting seat 8, an air guide plate 6 is fixed at the bottom end of the limiting seat 8, a controller 18 is installed at one end of the casing 11, an air suction pump 13 is installed at the top end of the casing 11, the input end of the air suction pump 13 is communicated with the top end inside the casing 11 through an air suction pipe 17, the first culture chamber 2 is arranged at one end inside the casing 11, the second culture chamber 10 is arranged at the other end inside the casing 11, heating wires 1 are laid inside the side wall of the casing 11, storage racks 3 are installed inside the first culture chamber 2 and the second culture chamber 10, a temperature sensor 12 and a vacuum sensor 15 are sequentially installed at the top end inside the casing 11, and an air inlet pipe 14 is fixed at one side inside the casing 11, the air inlet pipe 14 is provided with an electromagnetic valve 22, one side of the air inlet pipe 14, which is far away from the machine shell 11, is provided with a flange plate 23, the interior of the first culture chamber 2 is communicated with the interior of the second culture chamber 10, and the first culture chamber 2 and the second culture chamber 10 are symmetrically distributed relative to the vertical center line of the machine shell 11;

the structure of the device is optimized, and the stability of the whole structure of the device is improved through symmetrical distribution;

2 article placing frames 3 are arranged, two ends of each article placing frame 3 are welded with a reserved sliding strip 9, the outer side wall of each reserved sliding strip 9 is provided with an anti-slip rubber layer, and two ends of the interiors of the first culture chamber 2 and the second culture chamber 10 are provided with reserved sliding grooves 4 matched with the reserved sliding strips 9;

when the device is used, on one hand, the first culture chamber 2 and the second culture chamber 10 are arranged on the device, and the storage rack 3 is arranged in each culture chamber, so that two groups of placenta matrix mesenchymal stem cells are conveniently arranged for comparison culture by a user, the functionality of the device is enhanced, on the other hand, the storage rack 3 can be disassembled or assembled in the first culture chamber 2 and the second culture chamber 10 by the user by utilizing a sliding connection structure formed by the reserved sliding strips 9 and the reserved sliding grooves 4, the cultured storage rack 3 is convenient to take out, a new storage rack 3 to be cultured by the placenta matrix mesenchymal stem cells is replaced, and the convenience of the operation of the device is enhanced;

partition grooves 301 are uniformly fixed in the shelf 3, the partition grooves 301 are of a hollow structure, a silica gel protective layer is arranged on the inner side wall of each partition groove 301, and a sealing door is arranged on the casing 11;

the separation grooves 301 are uniformly fixed in the storage rack 3, so that a plurality of experimental samples can be conveniently arranged on mesenchymal stem cells of each group of placenta matrix, and the culture tubes filled with the experimental samples and culture solution are sequentially clamped into the separation grooves 301 for classified storage, so that the device can realize the culture of a plurality of samples, and the practicability of the device is enhanced;

four corners of the bottom end of the air guide plate 6 are all fixed with adsorption discs 5, the bottom end of each adsorption disc 5 is uniformly provided with adsorption holes, the inside of each adsorption disc 5 is communicated with the inside of the air guide plate 6, an integrated welding structure is formed between the air guide plate 6 and the limiting seat 8, and the input end of the vacuum pump 7 is communicated with the top end of the inside of the air guide plate 6 through a connecting pipe;

when the vacuum sucking device is used, a user can start the vacuum pump 7, the inside of the air guide plate 6 is vacuumized under the action of the connecting pipe, and the inside of the adsorption disc 5 communicated with the inside of the air guide plate 6 is vacuumized, so that negative pressure is generated inside the adsorption hole at the bottom end of the adsorption disc 5, and the corresponding operation table top is firmly adsorbed, thereby realizing the fixing and limiting effects on the device and enhancing the stability of the device;

the two ends of the bottom of the casing 11 are welded with connecting inserting strips 16, the top of the limiting seat 8 is uniformly provided with connecting slots 19 matched with the connecting inserting strips 16, one side of each connecting inserting strip 16 is welded with a locking sheet 20, and the locking sheet 20 is provided with a locking bolt 21 connected with the limiting seat 8;

when the device is used, a user can disassemble or assemble the shell 11 and the limiting seat 8 by utilizing an inserting connection structure formed by the connecting inserting strips 16 and the connecting inserting slots 19 and matching with the locking and fixing functions of the locking pieces 20 and the locking bolts 21, so that the device has the combined advantages and is convenient to clean and maintain;

the output ends of the temperature sensor 12 and the vacuum sensor 15 are respectively and electrically connected with the input end of the controller 18 through leads, the output end of the controller 18 is respectively and electrically connected with the input ends of the heating wire 1, the vacuum pump 7, the electromagnetic valve 22 and the air suction pump 13 through leads, the model of the electromagnetic valve 22 can be SV1000, the model of the air suction pump 13 can be BST-V550, the model of the vacuum pump 7 can be BYNFZ, the model of the controller 18 can be FX1S-20MR-D, the model of the temperature sensor 12 can be WRM-101, and the model of the vacuum sensor 15 can be HM 27.

The working principle is as follows: when the device is used, the device is externally connected with a power supply, a user firstly carries the device to a proper operation table board, then a vacuum pump 7 is started, the inside of the air guide plate 6 is vacuumized under the action of the connecting pipe, the inside of the adsorption disc 5 communicated with the inside of the air guide plate 6 is vacuumized, negative pressure is generated inside the adsorption hole at the bottom end of the adsorption disc 5, and the corresponding operation table board is firmly adsorbed, so that the device can be fixed and limited, the stability of the device is enhanced, the user can use the inserting connection structure formed by the connecting inserting strip 16 and the connecting slot 19 to match the locking and fixing action of the locking piece 20 and the locking bolt 21, the shell 11 and the limiting seat 8 are disassembled or assembled, the device further achieves the combined advantages, the cleaning and the maintenance are convenient, and then the air inlet pipe 14 is connected with the corresponding air guide equipment under the connecting action of the flange plate 23, finally, the cells are loaded into the device for culture, specifically, on one hand, a first culture chamber 2 and a second culture chamber 10 are arranged on the device, and each culture chamber is internally provided with a commodity shelf 3, so that a user can conveniently set two groups of placenta maternal mesenchymal stem cells for contrast culture, the functionality of the device is enhanced, on the other hand, the user can disassemble or assemble the commodity shelf 3 in the first culture chamber 2 and the second culture chamber 10 by utilizing a sliding connection structure formed by a reserved sliding strip 9 and a reserved sliding groove 4, so that the cultured commodity shelf 3 is convenient to take out, and the commodity shelf 3 to be cultured of new placenta maternal mesenchymal stem cells is replaced, the convenience of the operation of the device is enhanced, and a plurality of experimental samples are conveniently arranged on each group of placenta maternal mesenchymal stem cells by uniformly fixing separation grooves 301 in the commodity shelf 3, and each culture tube filled with experimental samples and culture solution is sequentially clamped into each separation groove 301 for classified storage, so that the device can realize the culture of a plurality of samples, the practicability of the device is enhanced, in addition, when the device is operated specifically, on one hand, the air suction pump 13 is started, the air in the shell 11 is sucked out under the action of the air suction pipe 17, meanwhile, the vacuum sensor 15 can constantly monitor the air condition in the shell 11 and send the monitored data to the controller 18, when the device is in a vacuum state, the controller 18 controls the air suction pump 13 to be closed and controls the electromagnetic valve 22 on the air inlet pipe 14 to be opened, the culture gas is led into the shell 11 through the air guide equipment, so that the device can regulate and control the air environment in the shell 11 to be beneficial to the growth of mesenchymal stem cells of the placenta, on the other hand, the heating wire 1 is controlled to be electrified, make it at the inside heat production of casing 11, meanwhile, temperature sensor 12 can monitor the inside gaseous condition of casing 11 constantly, and data transmission to monitoring gives controller 18, when the temperature reaches the default, controller 18 control heater strip 1 outage, and then make the device can the inside ambient temperature of accurate regulation and control, be convenient for create a suitable survival temperature for placenta matrix mesenchymal stem cell, thereby make the device can realize the regulation to gaseous environment and temperature environment, the functionality of device has been strengthened.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a culture apparatus that placenta matrix mesenchymal stem cell used, includes first culture chamber (2), spacing seat (8), second culture chamber (10) and casing (11), its characterized in that: the bottom of casing (11) is installed spacing seat (8), and the internally mounted of spacing seat (8) has vacuum pump (7), and the bottom mounting of spacing seat (8) has air guide plate (6), controller (18) are installed to the one end of casing (11), and the top of casing (11) installs aspiration pump (13), and the input of aspiration pump (13) is linked together through aspiration tube (17) and the inside top of casing (11), the inside one end of casing (11) is provided with first cultivation room (2), and the inside other end of casing (11) is provided with second cultivation room (10), and the inside of casing (11) lateral wall has laid heater strip (1), supporter (3) all are installed to the inside of first cultivation room (2) and second cultivation room (10), and temperature sensor (12) and vacuum sensor (15) are installed in proper order to the inside top of casing (11), and an air inlet pipe (14) is fixed on one side of the interior of the shell (11), an electromagnetic valve (22) is installed on the air inlet pipe (14), and a flange plate (23) is arranged on one side, away from the shell (11), of the air inlet pipe (14).

2. The culture apparatus for placental maternal mesenchymal stem cells according to claim 1, wherein: the interior of the first culture chamber (2) is communicated with the interior of the second culture chamber (10), and the first culture chamber (2) and the second culture chamber (10) are symmetrically distributed around the vertical center line of the machine shell (11).

3. The culture apparatus for placental maternal mesenchymal stem cells according to claim 1, wherein: supporter (3) are provided with 2, and the both ends of supporter (3) all weld and have reserved draw runner (9), and the lateral wall of reserving draw runner (9) sets up the non-slip rubber layer, and first cultivation room (2) and second cultivation room (10) inside both ends all are provided with and reserve draw runner (9) assorted reservation spout (4).

4. The culture apparatus for placental maternal mesenchymal stem cells according to claim 1, wherein: the inner part of the commodity shelf (3) is uniformly fixed with separation grooves (301), the separation grooves (301) are of a hollow structure, the inner side wall of each separation groove (301) is provided with a silica gel protective layer, and a sealing door is arranged on the machine shell (11).

5. The culture apparatus for placental maternal mesenchymal stem cells according to claim 1, wherein: four corners of air guide plate (6) bottom all are fixed with adsorption disc (5), and the bottom of adsorption disc (5) evenly is provided with the absorption hole, and the inside of adsorption disc (5) and the inside of air guide plate (6) are linked together, and constitute integration welded structure between air guide plate (6) and spacing seat (8), and the input of vacuum pump (7) is linked together through connecting pipe and the inside top of air guide plate (6).

6. The culture apparatus for placental maternal mesenchymal stem cells according to claim 1, wherein: the connecting inserting strips (16) are welded at two ends of the bottom of the shell (11), connecting slots (19) matched with the connecting inserting strips (16) are evenly formed in the top of the limiting seat (8), locking pieces (20) are welded on one sides of the connecting inserting strips (16), and locking bolts (21) connected with the limiting seat (8) are arranged on the locking pieces (20).