CN111893042B - Special constant temperature cell culture case of medical science experiment - Google Patents

Special constant temperature cell culture case of medical science experiment Download PDF

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Publication number
CN111893042B
CN111893042B CN202010745523.4A CN202010745523A CN111893042B CN 111893042 B CN111893042 B CN 111893042B CN 202010745523 A CN202010745523 A CN 202010745523A CN 111893042 B CN111893042 B CN 111893042B
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box body
placing
control valve
blocks
inner box
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CN111893042A (en
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李海军
刘菲
尹嘉宁
徐红梅
王中峰
李艳娇
高艳丽
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Jilin University
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Jilin University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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Abstract

The invention discloses a constant-temperature cell culture box special for medical experiments, which comprises an outer box body and a box door hinged with the outer box body, wherein an inner box body is arranged in the outer box body, the outer box body and the inner box body are respectively provided with a heat insulation block, a plurality of placing blocks are connected in the inner box body in a sliding manner, a plurality of placing grooves are respectively formed in the placing blocks, culture dishes are respectively arranged in the placing grooves, a heat insulation component and a displacement component are arranged in the placing blocks, a side box is arranged at the rear end of the outer box body, an adjusting component is arranged in the side box, and a control component is arranged in the inner box body. The work of temperature-sensing ware for control panel control heating piece work for the heat-conducting plate heats the heating fluid in the cavity, and then carries out thermostatic control to the culture dish, and because the specific heat capacity of heating fluid is great, when the external environment of culture dish contact, carries out temperature protection to the culture dish through the heat that contains in the heating fluid liquid hydrogen, when preventing a culture dish contact external environment, because abrupt temperature variation causes cell damage.

Description

Special constant temperature cell culture case of medical science experiment
Technical Field
The invention relates to cell culture equipment, in particular to a constant-temperature cell culture box special for medical experiments.
Background
When the traditional incubator takes out the culture dish, the temperature changes suddenly, so that the cells in the culture dish are damaged, and the tested cells can not accurately show the properties of the cells.
Disclosure of Invention
The invention aims to provide a constant-temperature cell culture box special for medical experiments to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a special constant temperature cell culture case of medical science experiment, includes outer box and articulated chamber door with it, the internal interior box that is equipped with of outer box, outer box and interior box all are equipped with the heat insulating block, sliding connection has a plurality of pieces of placing in the interior box, and is a plurality of all be equipped with a plurality of standing grooves in placing the piece, and is a plurality of all be equipped with the culture dish in the standing groove, it carries out heat retaining insulation assembly to the culture dish to place the piece in, the internal displacement subassembly that upwards drives the displacement to the culture dish that is equipped with of interior box, outer box rear end is equipped with the side case, the side incasement is equipped with the adjusting part that carries out balanced adjustment to the inside air carbon dioxide concentration of interior box, be equipped with a plurality of control assembly that carry out control to heat insulating part and adjusting part on the interior box.
As a further scheme of the invention: the placing blocks are provided with handles.
As a further scheme of the invention: the heat preservation subassembly includes cavity, heating liquid, heat-conducting plate, heating piece and temperature-sensing ware, and is a plurality of the cavity sets up respectively in the placing block of standing groove downside, be equipped with heating liquid in the cavity, the cavity downside is equipped with the heat-conducting plate, the heat-conducting plate downside is equipped with the heating piece, the standing groove side is equipped with the temperature-sensing ware.
As a further scheme of the invention: the displacement subassembly includes movable plate, spring, connecting plate, slider, chute and shifting chute, movable plate and standing groove sliding connection, the movable plate lower extreme passes through the spring to be connected with the standing groove bottom, placed the culture dish on the movable plate, it is a plurality of the movable plate passes through the connecting plate to be connected, the connecting plate tail end is equipped with the slider, slider and chute sliding connection, the chute sets up in the inner box, it is equipped with the shifting chute that supplies the connecting plate to remove to place the piece.
As a further scheme of the invention: the height of the chute is uniformly increased from inside to outside along the inner box body.
As a further scheme of the invention: the adjusting component comprises a carbon dioxide concentration sensor, an air storage tank, an air pump, a filter, a first pipeline, a first control valve, a second pipeline, a second control valve, a controller, a connecting pipe and an air inlet pipe, wherein the carbon dioxide concentration sensors are respectively arranged on the upper sides of the placing blocks, the air storage tank and the air pump are arranged in the side box, the filter which is contacted with the outside air is arranged on the rear side of the air pump, the output end of the air pump and the output end of the air storage tank are jointly communicated with the first pipeline, the first pipeline is provided with the first control valve, the first control valve is communicated with the inlet end of the second pipeline, a plurality of outlet ends of the second pipeline are respectively provided with the second control valve, the second control valve is provided with a controller for controlling the opening and closing of the second control valve, the second control valves are respectively communicated with one end of the connecting pipe, and the other end of the connecting pipe penetrates through the outer box body and the inner box body, the one end that the box was connected in the connecting pipe extends and the one end joint of intake pipe, and is a plurality of the intake pipe other end extends to in the chute respectively.
As a further scheme of the invention: the gas storage box is filled with carbon dioxide gas, and is provided with an air inlet connector.
As a further scheme of the invention: the control assembly comprises a control panel and a display, the display and the control panel are arranged on the outer side of the inner box body, and a plurality of buttons are arranged on the control panel.
As a further scheme of the invention: the control panel can control the operation of the heating block, the air pump, the first control valve and the second control valve.
As a further scheme of the invention: the box door is provided with a rubber ring, and the outer box body is provided with a connecting groove for accommodating the rubber ring.
Compared with the prior art, the invention has the beneficial effects that:
the temperature sensor works to enable the control panel to control the heating block to work, so that the heat conducting plate heats the heating liquid in the cavity, the culture dish is further subjected to constant temperature control, and due to the fact that the specific heat capacity of the heating liquid is large, when the culture dish is in contact with an external environment, the culture dish is subjected to temperature protection through heat contained in the liquid hydrogen of the heating liquid, and cell damage caused by sudden temperature change when the culture dish is in contact with the external environment is prevented; different control panels control the heating blocks in different placing blocks to work, and can control the temperature required by different cells in culture dishes in different placing blocks;
the carbon dioxide concentration sensor works to detect the carbon dioxide concentrations in different placing blocks, then the first control valve, the second control valve and the air pump are controlled through different control panels, and gas is transported through the first pipeline, the second pipeline, the connecting pipe and the air inlet pipe, so that the carbon dioxide concentrations in the different placing blocks are controlled;
through mutually supporting of spring, connecting plate, slider and chute for place the culture dish on the standing groove movable plate and can pop out by the autoload, and the culture dish when lower part in the standing groove, little with the distance of cavity, the required temperature of cell normal growth can effectively be supported.
Drawings
FIG. 1 is a front view of a thermostatic cell incubator specifically for use in medical experiments.
FIG. 2 is a schematic cross-sectional view of a thermostatic cell incubator specifically for use in medical experiments.
FIG. 3 is a partially enlarged schematic view of part A in FIG. 2 of a thermostatic cell incubator specifically for use in medical experiments.
FIG. 4 is a side sectional view of a thermostatic cell culture chamber special for medical experiments.
FIG. 5 is a partially enlarged schematic view of part B in FIG. 4 of a thermostatic cell incubator specifically for use in medical experiments.
FIG. 6 is a three-dimensional schematic view of a thermostatic cell incubator specifically for use in medical experiments.
In the figure: 1-outer box body, 2-box door, 3-rubber ring, 4-connecting groove, 5-inner box body, 6-placing block, 7-handle, 8-control panel, 9-display, 10-heat preservation block, 11-placing groove, 12-culture dish, 13-moving plate, 14-spring, 15-cavity, 16-heating liquid, 17-heat conducting plate, 18-heating block, 19-connecting plate, 20-sliding block, 21-chute, 22-moving groove, 23-carbon dioxide concentration sensor, 24-side box, 25-gas storage box, 26-air pump, 27-filter, 28-first pipeline, 29-first control valve, 30-second pipeline, 31-second control valve, 32-controller, 33-connecting pipe, 34-air inlet pipe and 35-temperature sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-6, a special constant temperature cell incubator for medical experiments, includes an outer box 1 and a box door 2 hinged thereto, an inner box 5 is provided in the outer box 1, the outer box 1 and the inner box 5 are both provided with heat insulation blocks 10, the heat insulation blocks 10 can effectively insulate the temperature of the inner box 5, four placing blocks 6 are slidably connected in the inner box 5, three placing grooves 11 are provided in the four placing blocks 6, culture dishes 12 are provided in the three placing grooves 11, heat insulation components for insulating the culture dishes 12 are provided in the placing blocks 6, a displacement component for driving the culture dishes 12 to move upwards is provided in the inner box 5, a side box 24 is provided at the rear end of the outer box 1, an adjusting component for balancing and adjusting the carbon dioxide concentration in the inner box 5 is provided in the side box 24, and four control components for controlling the heat insulation components and the adjusting component are provided on the inner box 5.
Furthermore, the four placing blocks 6 are provided with handles 7, so that the placing blocks 6 in the inner box body 5 can be conveniently drawn out;
the heat preservation component comprises a cavity 15, heating liquid 16, a heat conduction plate 17, a heating block 18 and a temperature sensor 35, wherein the cavities 15 are respectively arranged in the placing blocks 6 on the lower side of the placing groove 11, the heating liquid 16 is arranged in the cavity 15, the heating liquid 16 is liquid hydrogen with higher specific heat capacity, the heat conduction plate 17 is arranged on the lower side of the cavity 15, the heating block 18 is arranged on the lower side of the heat conduction plate 17, the temperature sensor 35 is arranged on the side end of the placing groove 11, the temperature sensors 35 on the four placing blocks 6 respectively work to detect the temperature of the placing grooves 11 in different placing blocks 6, the heating block 18 works to heat the heat conduction plate 17, the heat conduction plate 17 heats the heating liquid 16 in the cavity 15 in the placing block 6, and further performs the heat preservation function on cells in the culture dish 12 in the moving plate 6 placing groove 11, when the culture dish 12 moves out of the inner box body 5 along with the placing block 6, because the heating liquid 16 in the cavity 15 is liquid hydrogen with higher specific heat capacity, when the culture dish 12 is popped up, the temperature of the culture dish 12 is protected by the heat contained in the liquid hydrogen, and the cell damage caused by sudden temperature change when the culture dish 12 contacts the external environment is prevented;
the displacement assembly comprises moving plates 13, springs 14, connecting plates 19, sliders 20, chutes 21 and moving grooves 22, the moving plates 13 are slidably connected with the placing grooves 11, the lower ends of the moving plates 13 are connected with the bottoms of the placing grooves 11 through the springs 14, culture dishes 12 are placed on the moving plates 13, the moving plates 13 are connected through the connecting plates 19, the sliders 20 are arranged at the tail ends of the connecting plates 19, the sliders 20 are slidably connected with the chutes 21, the chutes 21 are arranged in the inner box body 5, the moving grooves 22 for the connecting plates 19 to move are formed in the placing blocks 6, when the placing blocks 6 are taken out, the holding blocks 6 are moved out of the inner box body 5 by pulling the handles 7, in the moving process, under the elastic force of the springs 14, the moving plates 13 move upwards to drive the connecting plates 19 to move upwards in the moving grooves 22 in the placing blocks 6, the sliders 20 at the tail ends of the connecting plates 19 move along the chutes 21, and the moving plates 13 move upwards in the placing grooves 11, driving the culture dish 12 to automatically pop up;
furthermore, the height of the chute 21 is uniformly increased from inside to outside along the inner box body 5, so that the culture dish 12 can be popped up;
the adjusting component comprises a carbon dioxide concentration sensor 23, an air storage tank 25, an air pump 26, a filter 27, a first pipeline 28, a first control valve 29, a second pipeline 30, a second control valve 31, a controller 32, a connecting pipe 33 and an air inlet pipe 34, wherein the four carbon dioxide concentration sensors 23 are respectively arranged on the upper sides of a plurality of placing blocks 6, the air storage tank 25 and the air pump 26 are arranged in the side tank 24, the filter 27 in contact with the outside air is arranged on the rear side of the air pump 26, the filter 27 can filter the air, the output end of the air pump 26 and the output end of the air storage tank 25 are communicated with the first pipeline 28 together, the first pipeline 28 is provided with the first control valve 29, the first control valve 29 is communicated with the inlet end of the second pipeline 30, the first control valve 29 is a three-way valve and can control the flow of the carbon dioxide and the air to the second pipeline 30, the four outlet ends of the second pipeline 30 are provided with the second control valves 31, the second control valves 31 are provided with controllers 32 for controlling the second control valves 31 to be opened and closed, the controllers 32 can control the content of gas passing through the second electromagnetic valves 31, the second control valves 31 are respectively communicated with one ends of the connecting pipes 33, the other ends of the connecting pipes 33 penetrate through the outer box body 1 and the inner box body 5, one ends of the connecting pipes 33 extending to the inner box body 5 are clamped with one ends of the gas inlet pipes 34, and the other ends of the gas inlet pipes 34 respectively extend into the inclined grooves 21;
further, the gas storage tank 25 is filled with carbon dioxide gas, the gas storage tank 25 is provided with a gas inlet interface, and when the concentration of the carbon dioxide in the gas storage tank 25 is insufficient, gas is added through the gas inlet interface 25;
further, when a large amount of oxygen is needed for the growth of the cells in the culture dish 12, the carbon dioxide gas in the gas storage tank 25 can be replaced by oxygen for oxygen transportation;
the control assembly comprises a control panel 8 and a display 9, the display 9 and the control panel 8 are arranged on the outer side of the inner box body 5, a plurality of buttons are arranged on the control panel 8, the temperature sensors 35 on the four placing blocks 6 work respectively to detect the temperatures of the placing grooves 11 in the different placing blocks 6, the carbon dioxide concentrations in the different placing blocks 6 are detected through the carbon dioxide concentration sensors 23, the detected data are displayed in the display screens 9 on the placing blocks 6, and the heating blocks 18 in the different placing blocks 6 and the first control valve 29 and the second control valve 31 in the side box 24 are controlled to work through the buttons on the control panel 8 according to the data in the different display screens 9;
further, the control panel 8 may control the operation of the heating block 18, the air pump 26, the first control valve 29, and the second control valve 31.
The working principle of the embodiment 1 of the invention is as follows:
when the device is used, a culture dish 12 with cultured cells is placed in the placing groove 11, the handle 7 is pulled to enable the placing blocks 6 to move into the inner box body 5, at the moment, the temperature sensors 35 on the four placing blocks 6 work respectively to detect the temperatures of the placing grooves 11 in different placing blocks 6, the carbon dioxide concentrations in different placing blocks 6 are detected through the carbon dioxide concentration sensors 23, the detected data are displayed in the display screens 9 on the placing blocks 6, and the heating blocks 18 in the different placing blocks 6 and the first control valve 29 and the second control valve 31 in the side box 24 are controlled to work through the buttons on the control panel 8 according to the data in the different display screens 9;
the heating block 18 works to heat the heat conducting plate 17, the heat conducting plate 17 heats the heating liquid 16 in the cavity 15 in the placing block 6, and then the cells in the culture dish 12 in the placing groove 11 of the moving plate 6 are subjected to heat preservation;
aiming at different carbon dioxide concentrations needed by different cells, the gas storage tank 25 in the side tank 24 conveys carbon dioxide in the gas storage tank into a second pipeline 30 through a first pipeline 28, the carbon dioxide is conveyed into different connecting pipes 33 from different output ends of the second pipeline 30, then the carbon dioxide is conveyed into a gas inlet pipe 34 through the connecting pipes 33, the carbon dioxide is conveyed into different placing blocks 6 through the gas inlet pipe 34 to provide carbon dioxide for cell growth, when the carbon dioxide concentration needs to be adjusted, the second control valve 31 is controlled through a control panel 8 control controller 32, the carbon dioxide content through the second control valve 31 is adjusted, when the carbon dioxide and waste gas generated by cell growth in the placing blocks 6 are replaced, the first control valve 29 is controlled through the control panel 8 to communicate the second pipeline 30 and the first pipeline 28 with an air pump 26, and the air pump 26 is communicated with the air pump 26 through the first pipeline 28, The second pipe 31, the connecting pipe 33 and the air inlet pipe 34 are replaced with the air outside the side box 24, and the air in the placing block 6 today is filtered by the filter 27, so that impurities can be prevented from entering the placing block 6;
when the placing block 6 needs to be taken out, the handle 7 is pulled to enable the placing block 6 to move out of the inner box body 5, in the moving process, under the elastic force action of the spring 14, the moving plate 13 moves upwards, the connecting plate 19 is further driven to move upwards in the moving groove 22 in the placing block 6, the sliding block 20 at the tail end of the connecting plate 19 moves along the inclined groove 21, the moving plate 13 moves upwards in the placing groove 11 to drive the culture dish 12 to automatically pop up, and in the popping-up process, the heating liquid 16 in the cavity 15 is liquid hydrogen with large specific heat capacity, when the culture dish 12 pops up, the temperature protection is carried out on the culture dish 12 through heat contained in the liquid hydrogen, and when the culture dish 12 contacts with the external environment, cell damage caused by sudden temperature change is prevented.
Example 2
Further improvement is made on the basis of the embodiment 1, and the improvement points are as follows: be equipped with rubber circle 3 on chamber door 2, be equipped with the spread groove 4 that holds rubber circle 3 on the outer box 1, the joint through rubber circle 3 and spread groove 4 makes chamber door 2 and outer box 1's sealed effect better for the cell in the culture dish 12 does not receive external disturbance in the outer box 1.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. The constant-temperature cell culture box special for medical experiments comprises an outer box body (1) and a box door (2) hinged with the outer box body, and is characterized in that an inner box body (5) is arranged in the outer box body (1), the outer box body (1) and the inner box body (5) are respectively provided with a heat insulation block (10), the inner box body (5) is connected with a plurality of placing blocks (6) in a sliding manner, a plurality of placing grooves (11) are respectively arranged in the placing blocks (6), culture dishes (12) are respectively arranged in the placing grooves (11), a heat insulation component for insulating the culture dishes (12) is arranged in the placing blocks (6), a displacement component for driving the culture dishes (12) to move upwards is arranged in the inner box body (5), a side box (24) is arranged at the rear end of the outer box body (1), and a regulating component for balancing and regulating the concentration of carbon dioxide in the air in the inner box body (5) is arranged in the side box (24), the inner box body (5) is provided with a plurality of control components for controlling the heat preservation component and the adjusting component;
the heat preservation assembly comprises cavities (15), heating liquid (16), heat conduction plates (17), heating blocks (18) and temperature sensors (35), the cavities (15) are respectively arranged in the placing blocks (6) on the lower side of the placing groove (11), the heating liquid (16) is arranged in the cavities (15), the heat conduction plates (17) are arranged on the lower sides of the cavities (15), the heating blocks (18) are arranged on the lower sides of the heat conduction plates (17), and the temperature sensors (35) are arranged on the side ends of the placing groove (11);
the displacement subassembly includes movable plate (13), spring (14), connecting plate (19), slider (20), chute (21) and shifting chute (22), movable plate (13) and standing groove (11) sliding connection, movable plate (13) lower extreme is connected bottom spring (14) and standing groove (11), placed culture dish (12) on movable plate (13), it is a plurality of movable plate (13) are connected through connecting plate (19), connecting plate (19) tail end is equipped with slider (20), slider (20) and chute (21) sliding connection, chute (21) set up in interior box (5), be equipped with shifting chute (22) that supply connecting plate (19) to remove in placing piece (6).
2. The incubator according to claim 1, wherein a plurality of said holding blocks (6) are provided with a handle (7).
3. The thermostatic cell incubator for medical experiment as claimed in claim 1, wherein the height of the inclined groove (21) is uniformly increased along the inner case (5) from the inside to the outside.
4. The incubator according to claim 1, wherein the adjusting assembly comprises a carbon dioxide concentration sensor (23), a gas storage tank (25), an air pump (26), a filter (27), a first pipeline (28), a first control valve (29), a second pipeline (30), a second control valve (31), a controller (32), a connecting pipe (33) and an air inlet pipe (34), wherein the carbon dioxide concentration sensors (23) are respectively disposed on the upper sides of the placing blocks (6), the gas storage tank (25) and the air pump (26) are disposed in the side tank (24), the filter (27) contacting with the outside air is disposed on the rear side of the air pump (26), the output end of the air pump (26) and the output end of the gas storage tank (25) are commonly communicated with the first pipeline (28), the first pipeline (28) is provided with the first control valve (29), first control valve (29) and the end intercommunication that advances of second pipeline (30), a plurality of play ends of second pipeline (30) all are equipped with second control valve (31), be equipped with on second control valve (31) and control its controller (32) of closing and opening, a plurality of second control valve (31) communicate with the one end of connecting pipe (33) respectively, the connecting pipe (33) other end runs through outer box (1) and interior box (5), the one end of box (5) and the one end joint of intake pipe (34) in connecting pipe (33) extend to, and is a plurality of intake pipe (34) other end extends to in chute (21) respectively.
5. The constant-temperature cell incubator special for the medical experiment as claimed in claim 4, wherein the gas storage tank (25) is filled with carbon dioxide gas, and the gas storage tank (25) is provided with a gas inlet connector.
6. The incubator according to claim 1, wherein the control assembly comprises a control panel (8) and a display (9), the display (9) and the control panel (8) are disposed outside the inner box (5), and the control panel (8) is provided with a plurality of buttons.
7. The isothermal cell incubator specific for medical experiments according to claim 6, characterized in that said control panel (8) controls the operation of the heating block (18), the air pump (26), the first control valve (29) and the second control valve (31).
8. The incubator according to any one of claims 1 to 7, wherein the chamber door (2) is provided with a rubber ring (3), and the outer case (1) is provided with a connecting groove (4) for accommodating the rubber ring (3).
CN202010745523.4A 2020-07-29 2020-07-29 Special constant temperature cell culture case of medical science experiment Active CN111893042B (en)

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