CN214496338U

CN214496338U - Multi-module continuous sample loading staged operation fluorescent quantitative PCR instrument

Info

Publication number: CN214496338U
Application number: CN202120365834.8U
Authority: CN
Inventors: 王冬
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-02-08
Filing date: 2021-02-08
Publication date: 2021-10-26
Anticipated expiration: 2031-02-08

Abstract

The utility model provides a multimodule is gone up appearance fluorescence quantitative PCR appearance of operation stage by stage in succession, including the detection case, the diapire is provided with a plurality of sample storehouses in the detection case, set up temperature control device in the sample storehouse, the inside upper end of detection case is provided with the collection subassembly, the collection subassembly contains screw drive. The utility model discloses in, through setting up collection subassembly and a plurality of sample storehouse, the sample can be placed to the homoenergetic in a plurality of sample storehouses, the collection subassembly can carry out data acquisition to the sample in the sample storehouse, and combine screw drive function, the collection subassembly can be accomplished sample storehouse data acquisition one by one, and a plurality of sample storehouses can not the independent operation of mutual influence with the collection subassembly, make this PCR appearance can realize the function that many batches of sample detected in succession, the problem that traditional PCR appearance only has single batch sample detection function has effectively been solved, the detection efficiency and the detection flux of this PCR appearance have been improved.

Description

Multi-module continuous sample loading staged operation fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a gene amplification technical field, in particular to a multi-module continuous sample loading stage-by-stage operation fluorescence quantitative PCR instrument.

Background

The fluorescence quantitative PCR technology is a technology for qualitatively or quantitatively detecting nucleic acid substances, and is mainly applied to molecular biology laboratories generally arranged in hospitals, disease control centers, life science research institutions, food safety detection research institutions, customs inspection and import and export quarantine and entry and exit travel health care centers, animal epidemic disease prevention and control centers, blood stations, seed stations arranged under the departments of agriculture and animal husbandry, aquatic product stations and the like. The application range is extremely wide, and compared with other methods such as a culture method, an ELASA method, a biochemical identification method and the like, the method is quicker and more sensitive. Related products are opened and sold by a plurality of domestic and international production enterprises.

The PCR amplification instrument is also called PCR gene amplification instrument, PCR nucleic acid amplification instrument, is an instrument for amplifying specific DNA by using PCR technology, and is widely used in medical and biological laboratories, for example, for judging whether a sample shows a map of a certain genetic disease, diagnosis of infectious diseases, gene replication, paternity test, etc., while the PCR instrument added with a fluorescent signal acquisition system and a computer analysis processing system on the basis of a common PCR instrument is called a fluorescence quantitative PCR instrument, and the PCR amplification principle is the same as that of the common PCR instrument, except that a primer added during PCR amplification is marked by using isotope, fluorescein, etc., and the primer and a fluorescent probe are simultaneously combined with a template for specific amplification.

At present, the main structure of the equipment sold on the market is a thermal cycling module, a thermal cover, an excitation/emission light path, a light source, a detector and a controller 6. The detection principle is that the DNA added with the reaction reagent can be melted, annealed and extended along with the temperature cycle change, the reaction is carried out once per temperature cycle, and the DNA of a single molecule can be copied once. The DNA content in the system is theoretically the initial amount multiplied by 2 to the power of N (the number of reactions). The molecules cannot be seen by eyes, a fluorescent marker is added to the DNA, the fluorescent signal is stronger and stronger along with the increasing quantity of the amplified DNA, and a curve of the increase of the quantity of the DNA can be calculated according to a change curve of the intensity of the fluorescent signal, so that whether the target DNA exists or not can be judged, and the initial existence quantity is approximate to what quantity

The existing fluorescence quantitative PCR instrument is designed into a single module, the sample loading PCR instrument needs to completely run the whole program every time, the running of the PCR instrument needs longer time, the single module design only has a single batch of sample detection function, sample loading cannot be continuously carried out, sample loading can be carried out next time only after the running of the PCR instrument is finished, the waiting time needs to be very long, manpower and time waste are easily caused, and the practicability is poor.

SUMMERY OF THE UTILITY MODEL

The utility model provides a multi-module is in succession to go up appearance fluorescence quantitative PCR appearance of moving stage by stage for solve present fluorescence quantitative PCR appearance and be the single module design, it all needs the whole procedure of complete operation to go up appearance PCR appearance at every turn, and PCR appearance operation completion needs longer time, because the single module design only has single batch sample test function, can't go up appearance in succession, only can wait for after the completion of PCR appearance operation to go up appearance next time after going up appearance, need wait for very long time, lead to artifical and time a large amount of wastes easily, the relatively poor technical problem of practicality.

In order to solve the technical problem, the utility model discloses a multimodule is in succession gone up appearance and is moved fluorescence quantitative PCR appearance stage by stage, include: the detection case, the diapire is provided with a plurality of sample storehouses in the detection case, set up temperature control device in the sample storehouse, the inside upper end of detection case is provided with gathers the subassembly.

Preferably, the collection assembly comprises: lead screw, slider, bar groove and first data acquisition device, the lead screw sets up the inside upper end of detection case, both ends respectively about the lead screw with the detection case left and right sides inner wall rotates the connection, threaded connection has the slider on the lead screw, the bar groove is seted up to detection case upside inner wall, the slider upper end extends to the bar inslot and with bar inslot wall sliding connection, the slider lower extreme is provided with the mounting panel, the mounting panel lower surface is provided with first data acquisition device.

Preferably, the lower surface of the sample chamber is slidably connected with the bottom wall of the detection box.

Preferably, the front surface of the sample bin is provided with a bin door, and the bin door is rotatably connected with one side wall of the sample bin.

Preferably, the outer wall of the left side of the detection box is provided with a forward and reverse rotation motor, and the output end of the forward and reverse rotation motor extends to the inside of the detection box and is fixedly connected with one end of the lead screw.

Preferably, the temperature control device includes: the temperature control device comprises an electric push rod, a temperature control box and an optical heat cover, wherein the fixed end of the electric push rod is fixedly connected with the inner bottom wall of the sample bin, one end of the electric push rod, which is far away from the inner bottom wall of the sample bin, is provided with the temperature control box, the outer wall of the temperature control box is slidably connected with the inner wall of the sample bin, a cavity is arranged in the temperature control box, the upper end of the cavity is communicated with the outside of the sample bin, a temperature controller is arranged in the cavity, the outer wall of the temperature controller is fixedly connected with the inner wall of the cavity, the lower surface of the temperature controller is provided with a radiator, the upper surface of the temperature controller is provided with a placing plate, the periphery of the placing plate is provided with a heat insulating layer, one side of the heat insulating layer is attached to the outer wall of the placing plate, the other side of the heat insulating layer is attached to the outer wall of the placing plate, the upper surface of the placing plate is provided with a plurality of sample grooves, samples to be detected are placed in the sample grooves, and the optical heat cover is arranged right above the temperature control box, one end of the optical thermal cover is fixedly connected with the inner wall of the rear side of the detection box.

Preferably, a display device is arranged at the upper end of the front side of the detection box, and the display device is electrically connected with the first data acquisition device, the forward and reverse rotating motor, the electric push rod, the temperature controller and the optical hot cover respectively.

Preferably, the collection assembly comprises a second data collection device, and the upper end of the second data collection device is fixedly connected with the upper end inside the detection box.

Preferably, the lower surface of the sample chamber is connected with the bottom wall of the detection box in a left-right sliding mode.

The technical scheme of the utility model has following advantage: the utility model provides a multimodule is gone up appearance fluorescence quantitative PCR appearance of operation stage by stage in succession, including the detection case, the diapire is provided with a plurality of sample storehouses in the detection case, set up temperature control device in the sample storehouse, the inside upper end of detection case is provided with the collection subassembly, contain lead screw drive in the collection subassembly. The utility model discloses in, through setting up collection subassembly and a plurality of sample storehouse, the sample can be placed to the homoenergetic in a plurality of sample storehouses, the collection subassembly can carry out data acquisition to the sample in the sample storehouse, and combine screw drive function, the collection subassembly can be accomplished sample storehouse data acquisition one by one, and a plurality of sample storehouses can not the independent operation of mutual influence with the collection subassembly, make this PCR appearance can realize the function that many batches of sample detected in succession, the problem that traditional PCR appearance only has single batch sample detection function has effectively been solved, the detection efficiency and the detection flux of this PCR appearance have been improved.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the apparatus particularly pointed out in the written description and drawings thereof.

The technical solution of the present invention is further described in detail by the accompanying drawings and examples.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a front view of the present invention;

FIG. 2 is a schematic view of the overall structure of the present invention;

FIG. 3 is a schematic view of the internal structure of the middle sample chamber of the present invention;

fig. 4 is another schematic structural diagram of the present invention.

In the figure: 1. a detection box; 2. a sample bin; 3. a lead screw; 4. a slider; 5. a strip-shaped groove; 7. a first data acquisition device; 8. a positive and negative rotation motor; 9. an electric push rod; 10. a temperature control box; 11. an optical thermal cover; 12. a cavity; 13. a temperature controller; 14. a heat sink; 15. placing the plate; 16. a heat insulating layer; 17. a sample tank; 18. a display device; 19. a second data acquisition device.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for description purposes, not specifically referring to the order or sequence, and are not intended to limit the present invention, but only to distinguish the components or operations described in the same technical terms, and are not to be construed as indicating or implying any relative importance or implicit indication of the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions and technical features between the various embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent, and is not within the protection scope of the present invention.

Example 1:

the embodiment of the utility model provides a multimode is in succession to be got ready fluorescence quantitative PCR appearance of stage operation, as shown in fig. 1-3, include: the detection case 1, the diapire is provided with a plurality of sample storehouses 2 in the detection case 1, set up temperature control device in the sample storehouse 2, the inside upper end of detection case 1 is provided with gathers the subassembly.

The working principle and the beneficial effects of the technical scheme are as follows: the inner bottom wall of the detection box 1 is provided with a plurality of sample bins 2, the number of the sample bins 2 can be four, samples to be detected can be placed in the four sample bins 2, meanwhile, the sample bins 2 are also internally provided with a temperature control device, the temperature control device can realize high-precision rapid automatic temperature rise and temperature reduction in the sample bins 2, the samples to be detected are firstly placed in the sample bins 2, then the temperature control device is started, the temperature of the temperature control device is raised to amplify the samples in the sample bins 2, when the amplification of the samples in one sample bin 2 of the sample bins 2 is completed, the acquisition component starts to acquire data of the sample bin 2 in which the amplification stage is completed, when the amplification stage in the other sample bins 2 is completed, the temperature control devices in the other sample bins 2 are rapidly cooled, so that the samples in the sample bins 2 which are amplified and do not start data acquisition are subjected to low-temperature protection, until the collection subassembly finishes the back of gathering, the collection subassembly carries out data acquisition to next sample storehouse 2, this scheme is through setting up collection subassembly and a plurality of sample storehouse 2, a plurality of sample storehouse 2 have realized the design of multimode, the sample can be placed to the homoenergetic in a plurality of sample storehouse 2, the collection subassembly can carry out data acquisition to the sample in the sample storehouse 2, and simultaneously, still be provided with temperature control device in the sample storehouse 2, combine temperature control device's automation to rise, the cooling function, make 2 insides in the sample storehouse move stage by stage, thereby make this PCR appearance can realize the function that many batches of samples detected in succession, the problem that traditional PCR appearance only has single batch of sample detection function has effectively been solved, and 2 circulation operations in a plurality of sample storehouses, a plurality of sample storehouses 2 and first data acquisition device 7 can not influence independent operation each other, the detection efficiency and the detection flux of this PCR appearance have been improved.

Example 2

On the basis of the above embodiment 1, the collecting assembly includes: lead screw 3, slider 4, bar groove 5, first data acquisition device 7, lead screw 3 sets up 1 inside upper end of detection case, lead screw 3 about both ends respectively with 1 left and right sides inner wall of detection case rotates the connection, threaded connection has slider 4 on the lead screw 3, bar groove 5 is seted up to 1 upside inner wall of detection case, 4 upper ends of slider extend to bar inslot 5 and with 5 inner wall sliding connection in bar groove, 4 lower extremes of slider are provided with the mounting panel, the mounting panel lower surface is provided with first data acquisition device 7.

The working principle and the beneficial effects of the technical scheme are as follows: when the collection assembly works, the screw rod 3 is rotated to enable the slide block 4 to slide along the strip-shaped groove 5, the slide block 4 drives the first data collection device 7 to move above the sample bin 2 through the mounting plate, the first data collection device 7 starts to collect data of samples in the sample bin 2, the first data collection device 7 is an existing device, the first data collection device 7 can be an immunodetection microfluidic chip fluorescence quantitative automatic detection device disclosed by CN 103901189B, when the amplification of the samples in the other sample bins 2 is finished under the action of temperature rise, if the first data collection device 7 finishes the data collection work of the sample bin 2, the screw rod 3 is rotated to enable the first data collection device 7 to accurately collect data of the other sample bin 2, and the temperature control devices in the other sample bins 2 are rapidly cooled to carry out low-temperature protection on the samples, then the sample in the first sample chamber 2 can be taken out and replaced, if the first data acquisition device 7 does not complete the data acquisition work of the sample chamber 2, the temperature control devices in the other sample chambers 2 which are amplified are rapidly cooled, so as to carry out low-temperature protection on the sample in the sample chamber 2 which is amplified and does not start data acquisition, through the arrangement of the first data acquisition device 7 and the plurality of sample chambers 2, the first data acquisition device 7 can complete the data acquisition on the sample in the sample chamber 2, the plurality of sample chambers 2 realize the multi-module design, the samples can be placed in the plurality of sample chambers 2, meanwhile, the temperature control device is also arranged in the sample chamber 2, the automatic temperature rising and reducing functions of the temperature control device are combined, so that the interior of the sample chamber 2 can run in stages, the PCR instrument can realize the continuous detection function of a plurality of batches of samples, and the problem that the traditional PCR instrument only has the single-batch sample detection function is effectively solved, and a plurality of sample storehouse 2 circulation operation, a plurality of sample storehouse 2 and first data acquisition device 7 can not influence independent operation each other, have improved the detection efficiency and the detection flux of this PCR appearance.

Example 3

On the basis of the above embodiment 2, the lower surface of the sample chamber 2 is slidably connected with the bottom wall of the detection box 1.

The working principle and the beneficial effects of the technical scheme are as follows: sliding connection around the bottom wall in 2 lower surfaces in sample storehouse and detection case 1 can adopt manual or electronic mode to pull out sample storehouse 2 from detection case 1, is convenient for place the sample that the configuration is reflected well to sample storehouse 2 inside, then can push sample storehouse 2 inside detection case 1 to the beginning operation detects the procedure or begins the operation amplification stage, and after the amplification stage was accomplished, first data acquisition device 7 removed extremely the beginning data acquisition in 2 tops in sample storehouse.

Example 4

On the basis of

embodiment

1 or 2, the front surface of the sample chamber 2 is provided with a chamber door, and the chamber door is rotatably connected with one side wall of the sample chamber 2.

The working principle and the beneficial effects of the technical scheme are as follows: sample storehouse 2 openly rotates and is provided with the door, and door one end rotates with 2 lateral walls in sample storehouse to be connected, and the door other end passes through mode fixed connection such as hasp with 2 opposite side inner walls in sample storehouse, and when 2 internal arrangement in sample storehouse appeared unusual, open the door, be convenient for overhaul 2 inside in sample storehouse.

Example 5

On the basis of any one of

embodiments

2 or 3, as shown in fig. 1, a forward and reverse rotation motor 8 is arranged on the outer wall of the left side of the detection box 1, and the output end of the forward and reverse rotation motor 8 extends into the detection box 1 and is fixedly connected with one end of the screw rod 3.

The working principle and the beneficial effects of the technical scheme are as follows: the left side wall of the detection box 1 is provided with a forward and reverse rotation motor 8, the model of the forward and reverse rotation motor 8 is 3IK15RGN-C, the output end of the forward and reverse rotation motor 8 is fixedly connected with one end of a lead screw 3, the axis is on the same straight line, when the forward and reverse rotation motor 8 rotates forwards, the lead screw 3 can be driven to rotate forwards, a slide block 4 is enabled to drive a first data acquisition device 7 to move along one side direction of the lead screw 3, so that the first data acquisition device 7 is aligned to one sample bin 2, when the forward and reverse rotation motor 8 rotates backwards, the lead screw 3 rotates backwards, the slide block 4 drives the first data acquisition device 7 to move towards the other side of the lead screw 3, the position of the first data acquisition device 7 is adjusted, and the first data acquisition device 7 is enabled to acquire data of the next sample bin 2.

Example 6

On the basis of embodiment 5, as shown in fig. 2 and 3, the temperature control device includes: the temperature control device comprises an electric push rod 9, a temperature control box 10 and an optical heat cover 11, wherein the fixed end of the electric push rod 9 is fixedly connected with the inner bottom wall of the sample bin 2, one end of the electric push rod 9, which is far away from the inner bottom wall of the sample bin 2, is provided with the temperature control box 10, the outer wall of the temperature control box 10 is slidably connected with the inner wall of the sample bin 2, a cavity 12 is arranged inside the temperature control box 10, the upper end of the cavity 12 is communicated with the outside of the sample bin 2, a temperature controller 13 is arranged in the cavity 12, the outer wall of the temperature controller 13 is fixedly connected with the inner wall of the cavity 12, the lower surface of the temperature controller 13 is provided with a radiator 14, the upper surface of the temperature controller 13 is provided with a placing plate 15, the periphery of the placing plate 15 is provided with a heat insulating layer 16, one side of the heat insulating layer 16 is attached to the outer wall of the placing plate 15, the other side of the heat insulating layer 16 is attached to the inner wall of the cavity 12, and the upper surface of the placing plate 15 is provided with a plurality of sample grooves 17, a sample to be detected is placed in the sample groove 17, the optical thermal cover 11 is arranged right above the temperature control box 10, and one end of the optical thermal cover 11 is fixedly connected with the inner wall of the rear side of the detection box 1.

The working principle and the beneficial effects of the technical scheme are as follows: the temperature control device comprises an electric push rod 9 and a temperature control box 10, the fixed end of the electric push rod 9 is fixedly connected with the bottom wall of the sample bin 2, the telescopic end of the electric push rod 9 is fixedly connected with the bottom wall of the temperature control box 10, the outer wall of the temperature control box 10 is slidably connected with the inner wall of the sample bin 2, under the push-pull action of the electric push rod 9, the temperature control box 10 can slide up and down along the inner wall of the sample bin 2 in the sample bin 2, a temperature controller 13 is arranged in the temperature control box 10, the model of the temperature controller 13 is OHR-A303, the temperature controller 13 can automatically heat up and cool down according to the temperature in the temperature control box 10, an optical heat cover 11 is arranged above the sample bin 2, the optical heat cover 11 adopts a heat cover special for a PCR instrument, the optical heat cover 11 is fixedly connected with the inner wall at the rear side of the detection box 1, after a sample is placed in a sample groove 17, the electric push rod 9 is started, the electric push rod 9 can push the temperature control box 10 to rise to the optical heat cover 11, the upper surface of the temperature control box 10 is tightly attached to the lower surface of the optical heat cover 11, the optical heat cover 11 is heated up first, when the optical heat cover 11 is heated up to 105 ℃, the temperature controller 13 is heated up automatically, the temperature controller 13 can perform temperature circulation automatically, under the common heating effect of the temperature controller 13 and the optical heat cover 11, rapid heating can be realized, the temperature inside the temperature control box 10 reaches the target temperature of PCR reaction, the temperature circulation process of the temperature controller 13 is heating up to 95 ℃ for melting, then cooling down to 60 ℃ for annealing, then heating up to 72 ℃ for extending, the complete PCR reaction needs to set 40-45 temperature circulation processes for the temperature controller 13, when the temperature circulation process is performed once, the first data acquisition device 7 acquires data, because the sample bins 2 are provided with a plurality of data, when the first data acquisition device 7 acquires data of a sample in one of the sample bins 2, other sample bins 2 can be loaded simultaneously or individually, then the sample is subjected to an amplification stage by an individual temperature control device in the sample bin 2, after the amplification stage is completed, if the first data acquisition device 7 is still in the data acquisition process, the temperature controller 13 in the sample bin 2 which completes the amplification stage automatically cools down for low-temperature protection, until the first data acquisition device 7 completes the data acquisition, the first data acquisition device 7 is controlled to move to the position above the sample bin 2 which is completed in the amplification stage and is not acquired for data acquisition, a plurality of sample bins 2 circularly operate, so that multi-module continuous sample loading is realized, the plurality of sample bins 2 operate in stages, the detection flux is improved, in the scheme, a closed space is formed between the optical heat cover 11 and the temperature control box 10, the evaporation of the sample can be effectively prevented, the accuracy of data acquisition is ensured, a sample groove 17 is arranged on the placing plate 15, for the sample provides and places the space, prevent that the sample from taking place to rock when data acquisition, placing 15 peripheries of board and setting up heat insulation layer 16, heat insulation layer 16 adopts heat insulating material, can prevent to place the temperature release in the board 15 outside, is provided with radiator 14 at temperature controller 13 lower surface, and radiator 14 adopts the venetian blind formula radiator, is favorable to the air flow in cavity 12, dispels the heat fast when being convenient for cool down.

Example 7

On the basis of embodiment 6, as shown in fig. 2, a display device 18 is disposed at the upper end of the front side of the detection box 1, and the display device 18 is electrically connected to the first data acquisition device 7, the forward and reverse rotation motor 8, the electric push rod 9, the temperature controller 13, and the optical thermal cover 11, respectively.

The working principle and the beneficial effects of the technical scheme are as follows: the upper end of the front side of the detection box 1 is provided with a display device 18, the display device 18 can adopt a TPC-M200 touch screen computer, the display device 18 is electrically connected with the first data acquisition device 7, data acquired by the first data acquisition device 7 can be directly uploaded to the display device 18 and displayed in a chart mode, a worker can conveniently and visually observe a data acquisition result and analyze the data, the display device 18 is also provided with a plurality of control buttons which respectively control the forward and reverse rotation of the forward and reverse rotation motor 8, the push and pull of the electric push rod 9, the automatic temperature rise and fall of the temperature controller 13 and the temperature rise of the optical heat cover 11, and the automation degree and the intelligent level of the equipment are improved.

Example 8

On the basis of the embodiment 1, the acquisition assembly comprises a second data acquisition device 19, and the upper end of the second data acquisition device 19 is fixedly connected with the upper end in the detection box 1

The working principle and the beneficial effects of the technical scheme are as follows: the second data acquisition device 19 is arranged in the detection box 1 and is fixedly connected with the upper end of the detection box 1, the second data acquisition device 19 comprises a detector, an emission optical filter, a dichroic mirror, a light source, an excitation optical filter and a lens group, the model of the detector is MC170HD, and the acquisition of the sample data in the sample bin 2 can be completed.

Example 9

On the basis of the embodiment 8, the lower surface of the sample chamber 2 is connected with the bottom wall of the detection box 1 in a left-right sliding manner.

The working principle and the beneficial effects of the technical scheme are as follows: 2 lower surfaces of sample storehouse and 1 inner bottom wall left and right sliding connection of detection case, after second data acquisition device 19 finishes to one of them sample storehouse 2 collection, the sample storehouse 2 that finishes will gather slides and keeps away from second data acquisition device 19 below, then slide another sample storehouse 2 and remove to second data acquisition device 19 below, start the data acquisition of next sample storehouse 2, detection case 1 is fixed motionless, sample storehouse 2 is changed at will, guarantee that a plurality of sample storehouses 2 can carry out the augmentation stage respectively, the augmentation finishes the back manual or remove sample storehouse 2 to second data acquisition device 19 below through conveyer, thereby accomplish data acquisition, the flexibility of data acquisition has been improved.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A multi-module continuous sample loading staged operation fluorescence quantitative PCR instrument is characterized by comprising: the detection box comprises a detection box body (1), wherein a plurality of sample bins (2) are arranged on the inner bottom wall of the detection box body (1), a temperature control device is arranged in each sample bin (2), and a collection assembly is arranged at the upper end inside the detection box body (1).

2. The multi-module fluorescence quantitative PCR instrument of claim 1, wherein the collection assembly comprises: lead screw (3), slider (4), bar groove (5), first data acquisition device (7), lead screw (3) set up the inside upper end of detection case (1), both ends about lead screw (3) respectively with detection case (1) left and right sides inner wall rotates the connection, threaded connection has slider (4) on lead screw (3), bar groove (5) are seted up to detection case (1) upside inner wall, slider (4) upper end extends to in bar groove (5) and with bar groove (5) inner wall sliding connection, slider (4) lower extreme is provided with the mounting panel, the mounting panel lower surface is provided with first data acquisition device (7).

3. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument as claimed in claim 2, wherein the lower surface of the sample chamber (2) is connected with the bottom wall of the detection box (1) in a sliding manner.

4. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument as claimed in claim 1, wherein the sample chamber (2) is provided with a chamber door at the front, and the chamber door is rotatably connected with one side wall of the sample chamber (2).

5. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument according to claim 2, wherein a forward and reverse rotation motor (8) is arranged on the outer wall of the left side of the detection box (1), and the output end of the forward and reverse rotation motor (8) extends into the detection box (1) and is fixedly connected with one end of the screw rod (3).

6. The fluorescence quantitative PCR instrument of claim 5, wherein the temperature control device comprises: electric putter (9), temperature control box (10) and optics heat lid (11), electric putter (9) stiff end with interior diapire fixed connection in sample storehouse (2), electric putter (9) keep away from in sample storehouse (2) diapire one end set up temperature control box (10), temperature control box (10) outer wall with interior wall sliding connection in sample storehouse (2), temperature control box (10) inside sets up cavity (12), cavity (12) upper end with the outside intercommunication in sample storehouse (2), set up temperature controller (13) in cavity (12), temperature controller (13) outer wall with cavity (12) inner wall fixed connection, temperature controller (13) lower surface is provided with radiator (14), temperature controller (13) upper surface is provided with places board (15), it is provided with heat insulation layer (16) to place board (15) periphery, heat insulation layer (16) one side with place board (15) outer wall laminating, the heat insulation layer (16) the other side with the laminating of cavity (12) inner wall, place board (15) upper surface and offer a plurality of sample groove (17), place in sample groove (17) and wait to detect the sample, optics heat lid (11) set up directly over temperature control box (10), optics heat lid (11) one end with detect case (1) rear side inner wall fixed connection.

7. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument according to claim 6, wherein a display device (18) is arranged at the upper end of the front side of the detection box (1), and the display device (18) is electrically connected with the first data acquisition device (7), the forward and reverse rotation motor (8), the electric push rod (9), the temperature controller (13) and the optical thermal cover (11) respectively.

8. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument as claimed in claim 1, wherein the collection assembly comprises a second data collection device (19), and the upper end of the second data collection device (19) is fixedly connected with the upper end of the interior of the detection box (1).

9. The multi-module continuous sample loading staged-operation fluorescence quantitative PCR instrument according to claim 8, wherein the lower surface of the sample chamber (2) is connected with the bottom wall of the detection box (1) in a sliding manner from side to side.