CN106434330A - Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor - Google Patents
Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor Download PDFInfo
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- CN106434330A CN106434330A CN201610881190.1A CN201610881190A CN106434330A CN 106434330 A CN106434330 A CN 106434330A CN 201610881190 A CN201610881190 A CN 201610881190A CN 106434330 A CN106434330 A CN 106434330A
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/061—Counting droplets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
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CN201610881190.1A CN106434330B (en) | 2016-10-09 | 2016-10-09 | A kind of absolute quantitation digital nucleic acid analysis system based on efficient drop microreactor |
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CN201610881190.1A CN106434330B (en) | 2016-10-09 | 2016-10-09 | A kind of absolute quantitation digital nucleic acid analysis system based on efficient drop microreactor |
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Cited By (29)
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CN107365685A (en) * | 2017-08-03 | 2017-11-21 | 甘肃出入境检验检疫局检验检疫综合技术中心 | Digital pcr amplification instrument and its method of work |
CN107497504A (en) * | 2017-07-24 | 2017-12-22 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Drop formation control device |
CN107860701A (en) * | 2017-12-22 | 2018-03-30 | 广东顺德墨赛生物科技有限公司 | Droplet type sample introduction fluoroscopic examination control system |
CN108220123A (en) * | 2018-01-29 | 2018-06-29 | 黄昶荃 | A kind of rapid and handy formula molecular detection devices based on real-time fluorescence quantitative PCR |
CN108315252A (en) * | 2018-03-30 | 2018-07-24 | 中国科学院天津工业生物技术研究所 | A kind of light-operated chip reaction system and method |
CN108373971A (en) * | 2017-03-11 | 2018-08-07 | 南京科维思生物科技股份有限公司 | Method and apparatus for carrying out real-time digital PCR |
CN108844936A (en) * | 2018-07-09 | 2018-11-20 | 领航基因科技(杭州)有限公司 | A kind of three-dimensional drop detection system and detection method |
CN109182092A (en) * | 2018-10-19 | 2019-01-11 | 苏州德思普生物科技有限公司 | A kind of micro-fluidic chip and its application for detection of nucleic acids |
CN109536380A (en) * | 2018-12-07 | 2019-03-29 | 王影珍 | A kind of the drop micro-fluidic chip and its application method of the highly sensitive detection of nucleic acid |
CN109536384A (en) * | 2018-12-24 | 2019-03-29 | 中国科学院上海微系统与信息技术研究所 | A kind of digital pcr system and its application for the quick absolute quantitation of nucleic acid |
CN109772485A (en) * | 2017-11-10 | 2019-05-21 | 上海新微技术研发中心有限公司 | Detection system based on micro-droplets |
CN109984841A (en) * | 2019-04-17 | 2019-07-09 | 南京医科大学 | The system for intelligently eliminating leg bone image spur using confrontation network model is generated |
CN110176277A (en) * | 2019-06-06 | 2019-08-27 | 福州大学 | A set of intelligent qPCR instrument detection system based on image recognition technology |
CN110261310A (en) * | 2019-07-10 | 2019-09-20 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory testing device |
CN110591911A (en) * | 2019-09-30 | 2019-12-20 | 中国科学院长春光学精密机械与物理研究所 | Optical-mechanical-electrical integrated full-automatic digital PCR device |
CN110702588A (en) * | 2019-09-25 | 2020-01-17 | 深圳先进技术研究院 | Digital PCR analysis device and PCR analysis method |
CN110982666A (en) * | 2019-12-19 | 2020-04-10 | 深圳市华迈生物医疗科技有限公司 | Device, system and method for real-time fluorescence quantitative nucleic acid amplification detection |
CN111215161A (en) * | 2020-01-15 | 2020-06-02 | 北京中科生仪科技有限公司 | Optical detection system for nucleic acid amplification instrument |
WO2020125246A1 (en) * | 2018-12-18 | 2020-06-25 | 苏州绘真生物科技有限公司 | Primers, probe, kit and detection method for detecting hepatitis b virus nucleic acid |
CN111378562A (en) * | 2020-03-20 | 2020-07-07 | 中国科学院长春光学精密机械与物理研究所 | Digital PCR detection quantitative system |
CN111394219A (en) * | 2020-03-20 | 2020-07-10 | 中国科学院长春光学精密机械与物理研究所 | Integrated digital PCR system |
CN112368562A (en) * | 2018-07-11 | 2021-02-12 | 香港大学 | Automatic microfluidic system for continuous and quantitative collection of droplets |
CN112439468A (en) * | 2019-08-30 | 2021-03-05 | 天津大学 | Rotary thermal circulation type multi-scale liquid drop digital polymerase chain reaction instrument system |
CN112630148A (en) * | 2020-12-16 | 2021-04-09 | 海南大学 | Integrated liquid drop digital PCR detection platform |
CN113462548A (en) * | 2021-06-24 | 2021-10-01 | 浙江大学 | Microfluidic chip for digital nucleic acid amplification and portable multi-channel detection system |
CN113956963A (en) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | Tiled chip used for liquid drop type digital PCR system and fluorescence detection system |
CN115060752A (en) * | 2022-07-27 | 2022-09-16 | 深圳市科誉仪器有限公司 | Automatic sample feeding device of X-ray fluorescence spectrometer for precious metal detection |
CN116042379A (en) * | 2023-04-03 | 2023-05-02 | 至美时代生物智能科技(北京)有限公司 | Device and method for preventing nucleic acid aerosol from being polluted |
CN116376668A (en) * | 2023-03-24 | 2023-07-04 | 中国科学院空间应用工程与技术中心 | Fully integrated nucleic acid detection chip, laminated structure thereof and detection method |
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CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微系统与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
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CN104388307A (en) * | 2014-11-24 | 2015-03-04 | 中国科学院苏州生物医学工程技术研究所 | Liquid drop type sample fluorescence detection system and method |
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Cited By (36)
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CN108373971A (en) * | 2017-03-11 | 2018-08-07 | 南京科维思生物科技股份有限公司 | Method and apparatus for carrying out real-time digital PCR |
CN107497504A (en) * | 2017-07-24 | 2017-12-22 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Drop formation control device |
CN107497504B (en) * | 2017-07-24 | 2019-11-08 | 广东永诺医疗科技有限公司 | Drop formation control device |
CN107365685A (en) * | 2017-08-03 | 2017-11-21 | 甘肃出入境检验检疫局检验检疫综合技术中心 | Digital pcr amplification instrument and its method of work |
CN109772485A (en) * | 2017-11-10 | 2019-05-21 | 上海新微技术研发中心有限公司 | Detection system based on micro-droplets |
CN107860701A (en) * | 2017-12-22 | 2018-03-30 | 广东顺德墨赛生物科技有限公司 | Droplet type sample introduction fluoroscopic examination control system |
CN108220123A (en) * | 2018-01-29 | 2018-06-29 | 黄昶荃 | A kind of rapid and handy formula molecular detection devices based on real-time fluorescence quantitative PCR |
CN108315252A (en) * | 2018-03-30 | 2018-07-24 | 中国科学院天津工业生物技术研究所 | A kind of light-operated chip reaction system and method |
CN108315252B (en) * | 2018-03-30 | 2023-09-05 | 中国科学院天津工业生物技术研究所 | Light control chip reaction system and method |
CN108844936A (en) * | 2018-07-09 | 2018-11-20 | 领航基因科技(杭州)有限公司 | A kind of three-dimensional drop detection system and detection method |
CN112368562A (en) * | 2018-07-11 | 2021-02-12 | 香港大学 | Automatic microfluidic system for continuous and quantitative collection of droplets |
CN109182092A (en) * | 2018-10-19 | 2019-01-11 | 苏州德思普生物科技有限公司 | A kind of micro-fluidic chip and its application for detection of nucleic acids |
CN109536380A (en) * | 2018-12-07 | 2019-03-29 | 王影珍 | A kind of the drop micro-fluidic chip and its application method of the highly sensitive detection of nucleic acid |
CN109536380B (en) * | 2018-12-07 | 2021-12-24 | 王影珍 | Droplet microfluidic chip for high-sensitivity detection of nucleic acid and use method thereof |
WO2020125246A1 (en) * | 2018-12-18 | 2020-06-25 | 苏州绘真生物科技有限公司 | Primers, probe, kit and detection method for detecting hepatitis b virus nucleic acid |
CN109536384A (en) * | 2018-12-24 | 2019-03-29 | 中国科学院上海微系统与信息技术研究所 | A kind of digital pcr system and its application for the quick absolute quantitation of nucleic acid |
CN109536384B (en) * | 2018-12-24 | 2022-02-18 | 中国科学院上海微系统与信息技术研究所 | Digital PCR system for rapid absolute quantification of nucleic acid and application thereof |
CN109984841A (en) * | 2019-04-17 | 2019-07-09 | 南京医科大学 | The system for intelligently eliminating leg bone image spur using confrontation network model is generated |
CN109984841B (en) * | 2019-04-17 | 2021-12-17 | 南京医科大学 | System for intelligently eliminating osteophytes of lower limb bone images by utilizing generated confrontation network model |
CN110176277A (en) * | 2019-06-06 | 2019-08-27 | 福州大学 | A set of intelligent qPCR instrument detection system based on image recognition technology |
CN110261310A (en) * | 2019-07-10 | 2019-09-20 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory testing device |
CN112439468A (en) * | 2019-08-30 | 2021-03-05 | 天津大学 | Rotary thermal circulation type multi-scale liquid drop digital polymerase chain reaction instrument system |
CN112439468B (en) * | 2019-08-30 | 2022-04-08 | 天津大学 | Rotary thermal circulation type multi-scale liquid drop digital polymerase chain reaction instrument system |
CN110702588B (en) * | 2019-09-25 | 2024-04-02 | 深圳市中科先见医疗科技有限公司 | Digital PCR analysis device and PCR analysis method |
CN110702588A (en) * | 2019-09-25 | 2020-01-17 | 深圳先进技术研究院 | Digital PCR analysis device and PCR analysis method |
CN110591911A (en) * | 2019-09-30 | 2019-12-20 | 中国科学院长春光学精密机械与物理研究所 | Optical-mechanical-electrical integrated full-automatic digital PCR device |
CN110982666A (en) * | 2019-12-19 | 2020-04-10 | 深圳市华迈生物医疗科技有限公司 | Device, system and method for real-time fluorescence quantitative nucleic acid amplification detection |
CN111215161A (en) * | 2020-01-15 | 2020-06-02 | 北京中科生仪科技有限公司 | Optical detection system for nucleic acid amplification instrument |
CN111394219A (en) * | 2020-03-20 | 2020-07-10 | 中国科学院长春光学精密机械与物理研究所 | Integrated digital PCR system |
CN111378562A (en) * | 2020-03-20 | 2020-07-07 | 中国科学院长春光学精密机械与物理研究所 | Digital PCR detection quantitative system |
CN112630148A (en) * | 2020-12-16 | 2021-04-09 | 海南大学 | Integrated liquid drop digital PCR detection platform |
CN113462548A (en) * | 2021-06-24 | 2021-10-01 | 浙江大学 | Microfluidic chip for digital nucleic acid amplification and portable multi-channel detection system |
CN113956963A (en) * | 2021-10-20 | 2022-01-21 | 西安天隆科技有限公司 | Tiled chip used for liquid drop type digital PCR system and fluorescence detection system |
CN115060752A (en) * | 2022-07-27 | 2022-09-16 | 深圳市科誉仪器有限公司 | Automatic sample feeding device of X-ray fluorescence spectrometer for precious metal detection |
CN116376668A (en) * | 2023-03-24 | 2023-07-04 | 中国科学院空间应用工程与技术中心 | Fully integrated nucleic acid detection chip, laminated structure thereof and detection method |
CN116042379A (en) * | 2023-04-03 | 2023-05-02 | 至美时代生物智能科技(北京)有限公司 | Device and method for preventing nucleic acid aerosol from being polluted |
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