CN101928663A - Integrated fluidic chip device for digital nucleic acid amplification and application - Google Patents
Integrated fluidic chip device for digital nucleic acid amplification and application Download PDFInfo
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Abstract
The invention provides an integrated fluidic chip device for digital nucleic acid amplification. The device consists of a vacuum system, a front pipeline, a buffer bottle, a rear pipeline, a capillary tube, a sucking disc, a sealing layer and a passage layer, wherein the passage layer and the sealing layer are sealed to form an integrated fluidic chip assembly; the passage layer is engraved with a passage and provided with a sample outlet and a sample inlet; the passage consists of a sinuous main passage and a plurality of side chambers positioned on two sides of the main passage; the two tail ends of the main passage are connected with the sample outlet and the sample inlet respectively; one end of the front pipeline is connected with the vacuum system, while the other end is connected with the buffer bottle; one end of the rear pipeline is connected with the buffer bottle, while the other end is connected with the capillary tube; and the capillary tube passes through the sucking disc and is communicated with the integrated fluidic chip assembly. The device has the advantages of reasonable design, microminiaturization, portability and simple operation, can distribute micro fluid into thousands of independent small chambers in dozens of seconds, reduce the complexity of chip manufacture and use and improve experiment speed, and can be applied in digital nucleic acid amplification.
Description
Technical field
The invention belongs to a plurality of fields proofing units such as life science, medical science, chemistry and engineering, relate to a kind of integrated fluidic chip device and application thereof that is used for digital nucleic acid amplification.
Background technology
Prove that now human numerous disease all has direct or indirect relation with gene, and gene is DNA (thymus nucleic acid) molecule fragment with hereditary effect.Therefore, the detection of nucleic acid and analysis not only are widely used at medical fields such as heredopathia, tumour and transmissible diseases, and also more and more important in the status in fields such as medical jurisprudence evaluation, food safety, archeology.Yet,, also be faced with more challenge simultaneously although nucleic acids research has been obtained the achievement that attracts people's attention.At first, the growth of organism, growth are determined by term single gene, but the result of a plurality of synergistic effect of gene is very complicated processes.Research gene diversity and problems such as transgenation or transformation have all been needed nucleic acid high-throughput rapid detection means badly.Secondly, the content of nucleic acid is very low, be difficult at present directly detect, and often adopt polymerase chain reaction (polymerase chain reaction, abbreviation PCR) technology at amplification in vitro DNA, up to target DNA concentration be enough to detected till.
Polymerase chain reaction technology invention is three more than ten years so far, and technology has obtained continuous development in the meantime, and the real-time fluorescence quantitative PCR of Chu Xianing (real-time quantitative PCR) technology has realized the leap of PCR from qualitative to quantitative in recent years.So-called real-time fluorescence quantitative PCR technology is meant in the PCR reaction system to add fluorophor, utilizes the fluorescent signal accumulation whole PCR process of monitoring in real time, the method for by typical curve template to be measured being carried out quantitative analysis at last.Yet this traditional quantifying PCR method also has its inevitable shortcoming: 1. the measuring point of quantitative fluorescent PCR is in the exponential phase of pcr amplification, and under the different templates, different condition, it is uncertain that pcr amplification reaches the needed cycle number of balance.If 2. the segmental initial concentration of purpose is low excessively, but often be difficult to the detection level that increases.3. the amplification efficiency of testing sample might and standard model variant.
1999, Vogelstein etc. have developed a kind of method that is called digital pcr (Digital PCR), with dna profiling to be measured dilution and be written into porous plate, make in average per two above holes and have only a template molecule, under optimized conditions, carry out after traditional thermal cycling PCR reaction, hybridize with the detection specificity amplified production with molecular beacon, positive hole produces fluorescence.Compare with traditional quantitative fluorescent PCR, digital pcr is the initial copy number that comes quantitative nucleic acid by the number in the positive hole of direct census, can accomplish absolute quantitation mensuration truly, therefore is referred to as digital pcr.Ottesen in 2006 etc. develop the micro-fluidic digital pcr chip that can carry out accurate quantification to a plurality of genes of bacterium, and the same year, U.S. Fluidigm company developed commercial digital array pcr chip, carried out nucleic acid amplification and detection by quantitative with the PCR in real time instrument.This digital pcr chip relates to a kind of little valve of PDMS elasticity based on the multilayer soft lithography prepared, and in this structure, fluid passage is arranged in the PDMS of lower floor layer, is made up of many parallel cells of connecting; The gas passage is arranged in upper strata PDMS, is made up of with the orthogonal passage in fluid channel many.This valve is not having external force to be in open state as the time spent, when exerting pressure (as air pressure or hydraulic pressure) to the gas passage, PDMS film between levels is expanded downwards, and valve is in closing condition, makes the subnatant circulation flow path be intercepted into several independently reaction chambers.
Above-mentioned digital pcr chip all has wide application prospect at Molecular Detection, non-invasive prenatal diagnosis, the genetic analysis of biology and the aspects such as disease relevant with the copy number variation of tumour.Editor doctor Blow of " Nature " and " Nature Method " magazine writes articles and points out that digital pcr is the new forward position of round pcr, can carry out unicellular even unit molecule research, realizes absolute quantitation by direct census.But the making of this digital pcr chip based on the little valve of PDMS elasticity relates to aligning, the sealing-in of multilayered structure, and the preparation process cycle is long and complicated, and present commercial chip and necessary instrument are all very expensive.Therefore, from the large-scale application prospect of digital pcr chip technology, present this preparation method and application cost all are disadvantageous.
Summary of the invention
The purpose of this invention is to provide a kind of with low cost, the integrated fluidic chip device that is used for digital nucleic acid amplification of easy handling, it is by vacuum system, preceding pipeline, surge flask, back pipeline, kapillary, sucker, sealing layer and channel layer are formed, channel layer and sealing layer carry out having formed the integrated fluidic chip assembly after the sealing-in, sucker places on the integrated fluidic chip assembly, channel layer is carved with passage, and be provided with outlet and injection port, passage is made up of the main channel of wriggling and some side rooms of being positioned at the both sides, main channel, two ends of main channel connect outlet and injection port respectively, the cross section in described side room can be square, circular, trapezoidal or Polygons, preceding pipeline one end connects vacuum system, the other end connects surge flask, back pipeline one end links to each other with surge flask, the other end links to each other with kapillary, and kapillary passes sucker and is communicated with the integrated fluidic chip assembly.
The size in main channel and side room can decide as required, and width of channel does not wait from 1 μ m to 1000 μ m, and preferred width range is 50 to 1000 μ m, and best scope is between 50 to 500 μ m, such as 50 μ m, 100 μ m, 200 μ m, 300 μ m, 400 μ m, 500 μ m; The depth range of passage 9 between 1 μ m to 1000 μ m, preferred range at 1 μ m between the 500 μ m, best scope at 10 μ m between the 100 μ m, such as 10 μ m, 20 μ m, 30 μ m, 50 μ m, 75 μ m, 100 μ m; The volume in side room is that skin liter (pl) is to receiving liter (nl) rank.
The making material of channel layer can be selected polymethylmethacrylate (PMMA), Resins, epoxy, tetrafluoroethylene, polystyrene (PC), glass, PDMS etc. for use.
The making of channel layer can be selected diverse ways for use according to different materials, such as methods such as laser ablation, chemical milling, photoetching, hot pressing, cast and injection mouldings.
The heat-resisting scotch tape of the material selection of sealing layer, PET film, fluorine film or with the flat board of channel layer same material or differing materials.
Channel layer and sealing layer carry out sealing-in, and method for sealing is according to material and use that difference can select that heat-resisting scotch tape is bonding for use, thermal bonding, heat-resistant adhesive is bonding or hot-press sealing etc.; After the sealing-in with adhesive tape with the porose sealing of institute with standby.
If what channel layer selected for use is non-transparent material, and sealing layer selects for use is mechanically resistant material, and then sealing layer needs bore aperture and aperture with the injection port and the corresponding position of outlet of channel layer in advance, and then carries out sealing-in.
If what channel layer selected for use is transparent material, the injection port that can be thereon and the position of outlet directly hole back and sealing layer sealing-in.
If what sealing layer selected for use is soft materials such as PET film, fluorine film or scotch tape, then sealing layer or channel layer all need not punching, only need in use to puncture with the position that syringe needle corresponds to injection port and outlet with film or adhesive tape to get final product.
The material of selecting for use according to the chip channel layer and the needs difference of experiment, material that can the back side of channel layer and thermal conductivitys such as aluminium flake, copper sheet or silicon chip are big is bonding.
The present invention also provides a kind of method of utilizing said apparatus to carry out the nucleic acid digital amplification, and described method may further comprise the steps:
(1) sample is prepared:
If nucleic acid amplification adopts traditional PCR method, then template DNA is mixed with SYBR Premix EXTaq test kit or Taqman probe reagent box;
If nucleic acid amplification adopts ring mediated isothermal amplification, then template DNA is mixed with LAMP reaction reagent and DNA fluorescence dye SYBR Green I;
(2) with the aperture on the sealing layer or the scotch tape at 11 places of the outlet on the channel layer punctures, and sucker is placed on this hole, begin then to vacuumize, make vacuum tightness reach 0～13332.2Pa, so just can utilize negative pressure to carry out sample introduction and microfluid distributes;
(3) according to the size in main channel and side room, at the aperture place on the chip sealing layer or the injection port place on the channel layer adds an amount of sample, and the adhesive tape of inciting somebody to action herein punctures, this moment, solution can enter the cell of main channel and both sides thereof under the effect of negative pressure, treat still to continue to vacuumize after solution is full of main channel and side room, liquid up to the main channel is drawn out of entirely, and the sample in side room can not be drawn out of and still kept this moment, is raised to and receives in the cell of upgrading thereby sample is separated into hundreds and thousands of skins; With a small amount of distilled water flushing main channel;
(4) in the main channel, import mineral oil and make it be full of the main channel, thereby thousands of side rooms are separated; Also can import lower boiling solution, thermosetting resin etc. in the main channel, purpose all is for the outlet in side room being blocked, avoiding heating the interior solution of rear side chamber and spread to the main channel;
(5) with solidifying glue or heat-resisting scotch tape with outlet on the aperture on the sealing layer and aperture or the channel layer and injection port sealing;
(6) the integrated fluidic chip assembly is placed original position PCR instrument or be similar on the thermocirculator of original position PCR instrument, can carry out traditional pcr amplification; If nucleic acid is carried out ring mediated isothermal amplification, the integrated fluidic chip assembly is placed on the common hot plate, 65 degree heating got final product in 30-60 minute;
(7) product after the amplification is carried out fluorescence imaging, software is counted positive hole.
Advantage of the present invention:
1. compare with the digital pcr that carries out on the porous plate, the present invention utilizes the characteristics of chip microminiaturization, and the consumption of reagent and sample has all seldom significantly reduced experimental cost; In addition, the present invention only needs to carry out Ngatively pressurized sampling by vacuumizing, and micro liquid is dispensed in hundreds and thousands of individual independently cells, has improved speed of experiment greatly.Demonstrate fully the development trend of current Analytical equipment microminiaturization, portability, had great advantage aspect high-throughput, low consumption and the extensive parallel processing.In addition,, directly do not contact, can effectively prevent outside contamination and crossed contamination with ambient atmosphere because the distribution of sample and reagent is all finished at chip internal.
2. the integrated fluidic chip among the present invention can experimental needs or different experiment condition and select different materials, of a great variety, select face width, for example polymethylmethacrylate (PMMA), tetrafluoroethylene, polystyrene (PC), polycarbonate (PC), Resins, epoxy, glass etc., if adopt the malleation sample introduction, can also select polydimethylsiloxane (PDMS) for use.
3. compare with existing commercialization digital pcr chip, the present invention does not need little valve that small amount of liquid is assigned in thousands of individual independently cells, has significantly reduced the complexity of chip manufacturing and use.
4. structure of the present invention and simple to operate, therefore no matter be chip or its required necessary instrument, its application cost all will significantly reduce than present commercial digital pcr chip based on the little valve of PDMS elasticity, for the popularization of digital pcr technology provides a good platform with using.
Description of drawings
Fig. 1 is a device synoptic diagram of the present invention.
Fig. 2 is the partial enlarged drawing of sucker and chip junction.
Fig. 3 is a chip channel layer synoptic diagram.
The positive hole of Fig. 4 Counting software work block diagram.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
Referring to Fig. 1, Fig. 2, Fig. 3, a kind of integrated fluidic chip device that is used for digital nucleic acid amplification, it is by vacuum system 1 (as vacuum pump), preceding pipeline 2, surge flask 3, back pipeline 4, kapillary 5, sucker 6, sealing layer 7 and channel layer 8 are formed, formed the integrated fluidic chip assembly after channel layer 8 and sealing layer 7 sealing-ins, sucker 6 places on the integrated fluidic chip assembly, channel layer 8 is carved with passage and injection port 12 and outlet 11, passage is made up of the main channel 9 of wriggling and some side rooms 10 of being positioned at 9 both sides, main channel, two ends of main channel 9 connect outlet 11 and injection port 12 respectively, preceding pipeline 2 one ends connect vacuum system 1, the other end connects surge flask 3, back pipeline 4 one ends link to each other with surge flask 3, the other end links to each other with kapillary 5, and kapillary 5 passes sucker 6 and is communicated with the integrated fluidic chip assembly.
If what sealing layer 7 was selected for use is mechanically resistant materials such as PMMA flat board, need aperture 13 and aperture 14 have been bored with injection port 12 corresponding positions at outlet 11 before the sealing-in with channel layer 8, and then carry out sealing-in, sealing-in success back with adhesive tape with the porose sealing of institute with standby;
If but sealing layer 7 selects for use is soft materials such as PET film, fluorine film, then need not to punch in advance, only need get final product with punctures with outlet 11 and injection port 12 opposite positions in use.
Outlet 11 reaches preceding pipeline 2 by corresponding aperture 13 on the chip sealing layer 7 with sucker 6, kapillary 5, back pipeline 4, surge flask 3 and vacuum system 1 links to each other (participating in Fig. 1,2).
Described vacuum system 1 is by vacuumizing, the sample suction main channel 9 and the side room 10 at aperture 14 places on the chip sealing layer 7 will be placed, after sample is full of main channel 9 and side room 10, continue to vacuumize, liquid in the main channel 9 can be extracted out, and the liquid in the side room 10 still keeps, thereby sample is separated in hundreds and thousands of cells of receiving upgrading.
Referring to Fig. 1, Fig. 2, Fig. 3, with reference to embodiment 1, a kind of integrated fluidic chip device that is used for digital nucleic acid amplification, it is by vacuum system 1 (as vacuum pump), preceding pipeline 2, surge flask 3, back pipeline 4, kapillary 5, sucker 6, sealing layer 7 and channel layer 8 are formed, formed the integrated fluidic chip assembly after channel layer 8 and sealing layer 7 sealing-ins, sucker 6 places on the integrated fluidic chip assembly, channel layer 8 is provided with passage and injection port 12 and outlet 11, passage is made up of the main channel 9 of wriggling and some side rooms 10 of being positioned at 9 both sides, main channel, and two ends of main channel 9 connect outlet 11 and injection port 12 respectively.Preceding pipeline 2 one ends connect vacuum system 1, and the other end connects surge flask 3, and back pipeline 4 one ends link to each other with surge flask 3, and the other end links to each other with kapillary 5, and kapillary 5 passes sucker 6 and is communicated with the integrated fluidic chip assembly.
If what sealing layer 7 was selected for use is mechanically resistant material, need bore aperture 13 and aperture 14 with injection port 12 corresponding positions at outlet 11 before the sealing-in, and then carry out sealing-in with channel layer 8, sealing-in success back is sealed the hole with standby with adhesive tape;
If but sealing layer 7 selects for use is soft materials such as PET film, fluorine film, then need not to punch in advance, only need get final product with punctures with outlet 11 and injection port 12 opposite positions in use.
Outlet 11 by corresponding aperture 13 on the chip sealing layer 7 and sucker 6, kapillary 5, back pipeline 4, surge flask 3 and before pipeline 2 link to each other with vacuum system 1 (referring to Fig. 1,2).
Described vacuum system 1 is by vacuumizing, with sample suction main channel 9 and the side room 10 on the aperture 14 that places on the chip sealing layer 7, after sample is full of main channel 9 and side room 10, continue to vacuumize, liquid in the main channel 9 can be extracted out, and the liquid in the side room 10 still keeps, thereby sample is separated in hundreds and thousands of cells of receiving upgrading.
The structure of the integrated fluidic chip device that is used for digital nucleic acid amplification of present embodiment is referring to Fig. 1, Fig. 2, Fig. 3, with reference to embodiment 1.It is made up of vacuum system 1 (as vacuum pump), preceding pipeline 2, surge flask 3, back pipeline 4, kapillary 5, sucker 6, sealing layer 7 and channel layer 8, formed the integrated fluidic chip assembly after channel layer 8 and sealing layer 7 sealing-ins, sucker 6 places on the integrated fluidic chip assembly, channel layer 8 is provided with passage and injection port 12 and outlet 11, passage is made up of the main channel 9 of wriggling and some side rooms 10 of being positioned at 9 both sides, main channel, and two ends of main channel 9 connect outlet 11 and injection port 12 respectively; Preceding pipeline 2 one ends connect vacuum system 1, and the other end connects surge flask 3, and back pipeline 4 one ends link to each other with surge flask 3, and the other end links to each other with kapillary 5, and kapillary 5 passes sucker 6 and is communicated with chip assembly.
After channel layer 8 is made, the drill bit of injection port 12 and outlet 11 usefulness diameter 1mm is got through, then with surface channel and 7 sealing-in of chip sealing layer; The glass of same nature adopts thermal bonding and channel layer 8 sealing-ins as chip sealing layer 7; Sealing-in success back is sealed injection port on the channel layer 8 12 and outlet 11 with standby with adhesive tape.
Outlet 11 links to each other with vacuum system 1 by sucker 6, kapillary 5, back pipeline 4, surge flask 3 and preceding pipeline 2; Described vacuum system 1 is by vacuumizing, the sample suction main channel 9 and the side room 10 at injection port 12 places will be placed, after sample is full of main channel 9 and side room 10, continue to vacuumize, liquid in the main channel 9 can be extracted out, and the liquid in the side room 10 still keeps, and rises or receives in the cell of upgrading thereby sample is separated into hundreds and thousands of skins.
Present embodiment adopts the device sample introduction of embodiment 1, and carries out follow-up digitizing ring mediated isothermal amplification.
Concrete steps:
1, the designed channel structure is referring to Fig. 3, and makes mask.
2, chip channel layer 8 is a material with the PMMA of 0.5mm, obtains required channel architecture through laser ablation, main channel 9 wide 100 μ m, dark 100 μ m; Side room 10 is shaped as square, and the length of side is 300 μ m, and the degree of depth is 0.5mm, promptly all carves logical.
The size in passage 9 and side room 10 can be selected as required, and the width of passage 9 does not wait to 1000 μ m from 10 μ m, and 10 μ m are dark to 1000 μ m; Side room 10 is of a size of nl to μ l level, and the side room can be square, circular or trapezoidal.
3, copper sheet that 0.6mm-2mm is thick is glued together with the back side of glue and chip channel layer 8.
With chip channel layer 8 back side adherent material except copper sheet, can also select thermal conductivity height such as nickel, silicon chip and not handle or after simple process, have the material of consistency with the reagent of nucleic acid amplification reaction.
4, with the surface channel and the heat-resisting scotch tape involution of chip channel layer 8.
5, adopt commercialization λ-DNA to investigate the performance of chip as reaction template: template DNA is diluted to suitable concentration and mixes with an amount of LAMP reaction reagent and DNA fluorescence dye SYBR Green I:
(1) λ-dna solution dilution with 500ng/ μ L is 5pg/ μ L, 0.5pg/ μ L, 0.05pg/ μ L, and 0.005pg/ μ L respectively gets 1 μ L as dna profiling and carries out the PCR reaction.
(2) according to 50uL system preparation LAMP reaction mixture: λ-dna profiling 1 μ L, primers F IP (40 μ M) 2 μ L, primer BIP (40 μ M) 2 μ L, primers F 3 (5 μ M) 2 μ L, primer B3 (5 μ M) 2 μ L, dNTP (2.5mM each) 8 μ L, MgSO
4(50mM) 6 μ L, betaine (5M) 10 μ L, Bst enzyme 2 μ L, Bstbuffer 5 μ L, SYBR Green I 2 μ L, ddH
2O 8 μ L.The LAMP primer is as follows:
F3：5’GTTGGGAAGGGCGATCG?3’
B3：5’ACTTTATGCTTCCGGCTCGTA?3’
FIP：5’ACAACGTCGTGACTGGGAAAACCCTTTTTGTGCGGGCCTCTTCGCTATTAC3’
BIP：5’CGACTCTAGAGGATCCCCGGGTACTTTTTGTTGTGTGGAATTGTGAGCGGAT?3’
6, the scotch tape corresponding to outlet 11 places on the chip sealing layer 7 is punctured, and sucker 6 is placed on this hole, begin then to vacuumize, make vacuum tightness reach 0～13332.2Pa.
7, on chip sealing layer 7, add the reaction mixture of 50 μ L corresponding to injection port 12 places, and the adhesive tape of inciting somebody to action herein punctures, this moment, solution can enter the cell 10 of main channel 9 and both sides thereof under the effect of negative pressure, treat still to continue to vacuumize after solution is full of main channel 9 and side room 10,9 liquid is drawn out of entirely up to the main channel, and this moment, the sample in side room 10 can not be drawn out of and still reservation; A small amount of distilled water flushing main channel 9.
8, in main channel 9, import mineral oil and make it be full of main channel 9, thereby thousands of side rooms 10 are separated; Also can be written into difficult volatile liquid, thermosetting resin in main channel 9, purpose all is for the outlet in side room 10 being blocked, avoiding heating rear side chamber 10 interior solution and spread to main channel 9.
9, with solidifying the thorn tear sealing of glue or heat-resisting scotch tape with adhesive tape.
10, chip assembly is placed on the hot plate, 65 degree heating 30 minutes are to carry out ring mediated isothermal amplification to nucleic acid.Because SYBR Green I fluorescence dye only combines with the double-stranded DNA ditch, when it during with the dna double chain combination, send original strong 800～1000 times fluorescence, therefore, the side room 9 of generation amplified reaction will present green fluorescence, feminine gender will not have colour-change.Therefore, if there is nucleic acid molecule to be assigned to certain independently side room 10, this side room will produce green fluorescence after reaction; If the concentration of nucleic acid is enough low, be that each side room 10 multipotency is assigned to a nucleic acid molecule, so, just can carry out accurate quantification to initial nucleic acid-templated amount by counting to positive hole.
11, to the amplification after product carry out fluorescence imaging after, with independently developed software to positive hole is counted and is analyzed.
Referring to Fig. 4, software by totalizer, original image reservoir, image calculation portion, examination criteria selection portion and as a result storage part etc. partly form.Original image reservoir: after data image enters process software, data are preserved, and in intermediate result calculating and net result output, provide the primary data; Image calculation portion: comprise committed steps such as rim detection, analysis of threshold, zone selection; Examination criteria selection portion: comprise several different examination criterias, carry out in sequence, with the processing requirements of the view data that adapts to different mass; Storage part as a result: be used for preserving the data of each result, and after executing all examination criterias, with result's output that adds up.
Present embodiment adopts the device sample introduction of embodiment 2, and carries out follow-up digitizing polymerase chain reaction.
Concrete steps:
1,, and makes mask referring to Fig. 3 designed channel structure;
2, chip channel layer 8 is a material with the heat resistant epoxide resin of 0.6mm, obtains required channel architecture through laser ablation, main channel 9 wide 100 μ m, dark 100 μ m; Side room 10 is shaped as square, and the length of side is 300 μ m, and the degree of depth is 0.4mm.
The size in passage 9 and side room 10 can be selected as required, and the width of passage 9 does not wait to 1000 μ m from 10 μ m, and 10 μ m do not wait deeply to 1000 μ m; Side room 10 is of a size of pl to the nl level, and the side room can be square, circular or trapezoidal.
3, aluminium sheet that 0.6mm-2mm is thick is glued together with the back side of glue and chip channel layer 8.
With chip channel layer 8 back side adherent material except aluminium sheet, can also select thermal conductivity height such as copper sheet, nickel, silicon chip and not handle or after simple process, have the material of consistency with the reagent of nucleic acid amplification reaction.
4, be chip sealing layer 7 with heat-resisting fluorine film or PET film, hot pressing and 8 sealing-in of chip channel layer after heat-resistant adhesive is bonding;
5, adopt commercialization λ-DNA to investigate the performance of chip as reaction template: template DNA is diluted to suitable concentration and mixes with an amount of quantitative fluorescent PCR reaction reagent:
1) λ of 500ng/ μ L-dna solution dilution is 5pg/ μ L, O.5pg/ μ L, 0.05pg/ μ L, 0.005pg/ μ L respectively gets 2 μ L as dna profiling and carries out the PCR reaction.
2) use SYBR PremixEX Taq test kit to carry out pcr amplification reaction, according to 50 μ L volumes preparation reaction solution, SYBR Premix EX Taq (2 *) 25 μ L, PCR Forward Primer (10 μ M) 1 μ L, PCR Reverse Primer (10 μ M) 1 μ L, ddH
2O 21 μ L, dna profiling 2.0 μ L.Reaction conditions is: 95 ℃ of pre-sex change of 30sec, 95 ℃ of 5sec, 60 ℃ of 30sec, totally 40 circulations.The primer sequence of used λ-DNA is as follows:
Upstream primer: 5 ' GATGAGTTCGTGTCCGTACAACTGG 3 '
Downstream primer: 5 ' GGTTATCGAAATCAGCCACAGCGCC 3 '
The amplified reaction of PCR and detection can also adopt the Taqman probe to finish.
6, the film corresponding to outlet 11 places on the chip sealing layer 7 is punctured, and sucker 6 is placed herein, begin then to vacuumize, make vacuum tightness reach 0～13332.2Pa.
7, on chip sealing layer 7, add the reaction mixture of 50 μ L corresponding to injection port 12 places, and film punctures herein, this moment, solution can enter the cell 10 of main channel 9 and both sides thereof under the effect of negative pressure, treat still to continue to vacuumize after solution is full of main channel 9 and side room 10,9 liquid is drawn out of entirely up to the main channel, and the sample in side room 10 can not be drawn out of face and still kept this moment; A small amount of distilled water flushing main channel 9;
8, in main channel 9, be written into mineral oil and make it be full of main channel 9, thereby thousands of side rooms 10 are separated; Also can be written into low-boiling point liquid, thermosetting resin etc. in main channel 9, purpose all is for the outlet in side room 10 being blocked, avoiding heating rear side chamber 10 interior solution and spread to main channel 9.
9, with solidifying the thorn tear sealing of glue or heat-resisting scotch tape with film.
10, chip assembly is placed on the original position PCR instrument 95 ℃ of pre-sex change of 30sec, 95 ℃ of 5sec, 60 ℃ of 30sec, totally 40 circulations.Because SYBR Green I fluorescence dye only combines with the double-stranded DNA ditch, when it during with the dna double chain combination, send original strong 800～1000 times fluorescence, therefore, the side room 9 of generation amplified reaction will present green fluorescence, feminine gender will not have colour-change.Therefore, if there is nucleic acid molecule to be assigned to certain independently side room 10, this side room will produce green fluorescence after reaction; If the concentration of nucleic acid is enough low, make each side room 10 multipotency assign to a nucleic acid molecule, so, just can carry out accurate quantification to initial nucleic acid-templated amount by counting to positive hole.
11, to the amplification after product carry out fluorescence imaging after, by software positive hole is counted and is analyzed, referring to Fig. 4.
Claims (10)
1. integrated fluidic chip device that is used for digital nucleic acid amplification, by vacuum system (1), preceding pipeline (2), surge flask (3), back pipeline (4), kapillary (5), sucker (6), sealing layer (7) and channel layer (8) are formed, road layer (8) carries out sealing-in with sealing layer (7) and forms the integrated fluidic chip assembly, logical, sucker (6) places on the integrated fluidic chip assembly, channel layer (8) is carved with passage, and be provided with outlet (11) and injection port (12), passage is made up of the main channel (9) of wriggling and some side rooms (10) of being positioned at both sides, main channel (9), two ends of main channel (9) connect outlet (11) and injection port (12) respectively, the cross section in side room (10) is a square, circular, trapezoidal or Polygons, one end of preceding pipeline (2) connects vacuum system (1), the other end connects surge flask (3), one end of back pipeline (4) links to each other with surge flask (3), the other end links to each other with kapillary (5), and kapillary (5) passes sucker (6) and is communicated with the integrated fluidic chip assembly.
2. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 1, it is characterized in that, the width of main channel (9) is from 1 μ m to 1000 μ m, and depth range is between 1 μ m to 1000 μ m, and the volume of side room (10) is that skin is raised to and receives upgrading not.
3. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 1, it is characterized in that, a kind of among material selection polymethylmethacrylate, Resins, epoxy, tetrafluoroethylene, polystyrene, glass or the PDMS of channel layer (8), the making method of channel layer (8) is selected laser ablation method, chemical method for etching, photolithography, pressure sintering, casting or injection moulding for use.
4. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 1 is characterized in that, the heat-resisting scotch tape of material selection of sealing layer (7), PET film, fluorine film or with the flat board of channel layer (8) same material or differing materials;
5. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 1 is characterized in that, the method for sealing of channel layer (8) and sealing layer (7) selects that heat-resisting scotch tape is bonding for use, thermal bonding, heat-resistant adhesive is bonding or the hot-press sealing method.
6. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 5, it is characterized in that, when channel layer (8) is selected non-transparent material for use, sealing layer (7) is when selecting mechanically resistant material for use, sealing layer (7) needs boring aperture (14) and aperture (13) with injection port (12) and the corresponding position of outlet (11) in advance, and then carry out sealing-in, after the sealing-in with adhesive tape with the porose sealing of institute with standby.
7. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 5, it is characterized in that, when channel layer (8) is selected transparent material for use, then directly hole in the position of injection port (12) and outlet (11) back with sealing layer 7 sealing-ins, after the sealing-in with adhesive tape with the porose sealing of institute with standby.
8. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification according to claim 5, it is characterized in that, when sealing layer (7) is selected soft material for use, then sealing layer (7) or channel layer (8) all need not punching, only need in use to puncture with the position that syringe needle corresponds to injection port (12) and outlet (11) with film or adhesive tape and get final product, soft material is selected PET film, fluorine film or transparent adhesive tape for use.
9. a kind of integrated fluidic chip device that is used for digital nucleic acid amplification of stating according to claim 1 is characterized in that, the material that the back side of channel layer (8) and thermal conductivity are big is bonding, material selection aluminium flake, copper sheet or silicon chip that thermal conductivity is big.
10. a kind of application that is used for the integrated fluidic chip device of digital nucleic acid amplification of stating according to claim 1 is characterized in that, realizes by following steps:
(1) sample is prepared:
If nucleic acid amplification adopts traditional PCR method, then template DNA is mixed with SYBR Premix EXTaq test kit or Taqman probe reagent box;
If nucleic acid amplification adopts ring mediated isothermal amplification, then template DNA is mixed with LAMP reaction reagent and DNA fluorescence dye SYBR Green I;
(2) scotch tape that the aperture (13) on the sealing layer (7) or the outlet (11) on the channel layer (8) are located punctures, and sucker (6) is placed on this hole, begins then to vacuumize, and makes vacuum tightness reach 0～13332.2Pa;
(3) the aperture (14) on the chip sealing layer (7) locate or channel layer (8) on injection port (12) locate to add sample, and the adhesive tape of inciting somebody to action herein punctures, this moment, solution can enter the cell (10) of main channel (9) and both sides thereof under the effect of negative pressure, treat still to continue to vacuumize after solution is full of main channel (9) and side room (10), the liquid of (9) is drawn out of entirely up to the main channel, with a small amount of distilled water flushing main channel (9);
(4) in main channel (9), import mineral oil and make it be full of main channel (9), thereby thousands of side rooms (10) are separated; Or in main channel (9), import lower boiling solution or thermosetting resin, the outlet of blocking side room (10);
(5) with solidifying glue or heat-resisting scotch tape with outlet (11) on the aperture (13) on the sealing layer (7) and aperture (14) or the channel layer (8) and injection port (12) sealing;
(6) the integrated fluidic chip assembly is placed original position PCR instrument or be similar on the thermocirculator of original position PCR instrument, carry out traditional pcr amplification; If nucleic acid is carried out ring mediated isothermal amplification, the integrated fluidic chip assembly is placed on the common hot plate, 65 degree heating got final product in 30-60 minute;
(7) product after the amplification is carried out fluorescence imaging, software is counted positive hole.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102277294A (en) * | 2011-08-03 | 2011-12-14 | 浙江大学 | High-density array chip device used for digital nucleic acid amplification application of device |
| CN102669058A (en) * | 2011-03-18 | 2012-09-19 | 中国科学院大连化学物理研究所 | Micro valve-based microfluidic chip for long-term culture and dual detection of caenorhabditis elegans |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127191A2 (en) * | 2005-04-20 | 2006-11-30 | Fluidigm Corporation | Analysis engine and database for manipulating parameters for fluidic systems on a chip |
| WO2007051170A2 (en) * | 2005-10-28 | 2007-05-03 | The Regents Of The University Of California | Apparatus and method for improved optical detection of particles in fluid |
| US20080024576A1 (en) * | 2006-07-25 | 2008-01-31 | Samsung Electronics Co., Ltd. | Mobile image forming apparatus |
| WO2010011852A1 (en) * | 2008-07-25 | 2010-01-28 | Fluidigm Corporation | Method and system for manufacturing integrated fluidic chips |
| CN201901669U (en) * | 2010-07-23 | 2011-07-20 | 浙江大学 | Integrated flow path chip device for digital nucleic acid amplification |
-
2010
- 2010-07-23 CN CN 201010235454 patent/CN101928663B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127191A2 (en) * | 2005-04-20 | 2006-11-30 | Fluidigm Corporation | Analysis engine and database for manipulating parameters for fluidic systems on a chip |
| WO2007051170A2 (en) * | 2005-10-28 | 2007-05-03 | The Regents Of The University Of California | Apparatus and method for improved optical detection of particles in fluid |
| US20080024576A1 (en) * | 2006-07-25 | 2008-01-31 | Samsung Electronics Co., Ltd. | Mobile image forming apparatus |
| WO2010011852A1 (en) * | 2008-07-25 | 2010-01-28 | Fluidigm Corporation | Method and system for manufacturing integrated fluidic chips |
| CN201901669U (en) * | 2010-07-23 | 2011-07-20 | 浙江大学 | Integrated flow path chip device for digital nucleic acid amplification |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669058A (en) * | 2011-03-18 | 2012-09-19 | 中国科学院大连化学物理研究所 | Micro valve-based microfluidic chip for long-term culture and dual detection of caenorhabditis elegans |
| CN102277294A (en) * | 2011-08-03 | 2011-12-14 | 浙江大学 | High-density array chip device used for digital nucleic acid amplification application of device |
| CN107615039A (en) * | 2015-05-26 | 2018-01-19 | 奥林巴斯株式会社 | Check body dyeing apparatus and check body colouring method |
| CN105505761A (en) * | 2015-12-21 | 2016-04-20 | 中国科学院苏州生物医学工程技术研究所 | Digital isothermal nucleic acid detecting device and detecting method thereof |
| CN106434330B (en) * | 2016-10-09 | 2019-01-08 | 苏州昊通仪器科技有限公司 | A kind of absolute quantitation digital nucleic acid analysis system based on efficient drop microreactor |
| CN106434330A (en) * | 2016-10-09 | 2017-02-22 | 戴敬 | Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor |
| CN108359600A (en) * | 2018-01-22 | 2018-08-03 | 中国科学院苏州生物医学工程技术研究所 | A kind of highly sensitive, quick, absolute quantitation DNA fragmentation detecting system and detection method |
| CN110396472A (en) * | 2018-04-25 | 2019-11-01 | 光行科技株式会社 | Box for digital PCR in real time |
| US11426731B2 (en) | 2018-04-25 | 2022-08-30 | Optolane Technologies Inc. | Cartridge for digital real-time PCR |
| CN108459172A (en) * | 2018-06-05 | 2018-08-28 | 江苏卓微生物科技有限公司 | Micro-fluidic chip is loaded sealing system and the cytoanalyze comprising it |
| CN109182469A (en) * | 2018-08-24 | 2019-01-11 | 暨南大学 | Primer and kit and method based on digital LAMP technology detection Enterobacter sakazakii |
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