CN214496272U - Improvement device that agrobacterium infects plant callus - Google Patents

Abstract

An improved device for plant callus impregnated by agrobacterium comprises an outer bottle for containing agrobacterium solution, an inner bottle sleeved in the outer bottle, a plurality of through holes arranged on the inner bottle, and a discharge pipe arranged at the bottom of the outer bottle; the vacuum-pumping mechanism is arranged at the bottleneck of the inner bottle; and a stirring mechanism for driving the agrobacterium solution in the outer bottle to rotate is also arranged in the outer bottle. This application can make plant material fully dip-dye, improves conversion efficiency, and the discharge pipe that the bottom set up can be taken the unnecessary moisture in the bottle out, reduces miscellaneous fungus and pollutes, and the evacuating device that the top set up can get rid of the unnecessary moisture in the bottle as fast as possible, avoids miscellaneous fungus to pollute.

Description

Improvement device that agrobacterium infects plant callus

Technical Field

The application relates to an improvement device of agrobacterium infects plant callus.

Background

At present, agrobacterium-mediated genetic transformation is mainly to put receptor materials into an outer bottle and add agrobacterium liquid for dip dyeing for a period of time. Then pouring out the dip-dyeing bacteria liquid, taking out the receptor material, putting the receptor material into sterile filter paper, sucking off excessive moisture, and blowing the receptor material for a period of time on a super clean bench until no obvious moisture exists on the surface of the receptor material. The main drawbacks of this operation are: firstly, in the dip-dyeing process, plant materials cannot be fully dip-dyed, so that the conversion efficiency is low; secondly, the dip dyeing time is uncertain, a timer is needed, and the contamination of infectious microbes is easily caused; when the redundant bacteria liquid is poured, the receptor material in the outer bottle is easy to pour out together, so that the experimental material is wasted; fourthly, when the receptor material is taken out and is placed in sterile filter paper to blow and dry excess moisture, the contamination of mixed bacteria is easy to cause.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the application provides an improved device for impregnating agrobacterium with plant callus, which comprises an outer bottle containing agrobacterium solution, wherein an inner bottle is sleeved in the outer bottle, a plurality of through holes are formed in the inner bottle, and a discharge pipe is arranged at the bottom of the outer bottle; the vacuum-pumping mechanism is arranged at the bottleneck of the inner bottle; and a stirring mechanism for driving the agrobacterium solution in the outer bottle to rotate is also arranged in the outer bottle. This application can make plant material fully dip-dye, improves conversion efficiency, and the discharge pipe that the bottom set up can be taken the unnecessary moisture in the bottle out, reduces miscellaneous fungus and pollutes, and the evacuating device that the top set up can get rid of the unnecessary moisture in the bottle as fast as possible, avoids miscellaneous fungus to pollute.

Preferably, interior bottle includes cylindrical bottle, the closed setting in bottom of interior bottle, the lateral part of interior bottle is equipped with a plurality of ways and runs through the bar hole that sets up in order to form the through-hole from the top down.

Preferably, the strip-shaped holes are obliquely arranged, and the oblique direction of the strip-shaped holes is consistent with the direction in which the stirring mechanism drives the agrobacterium solution to rotate; the included angle between the axis of the strip-shaped hole and the horizontal direction is not less than 60 degrees, and the width of the strip-shaped hole is not more than 1 mm. The conditional hole of this application can be so that whether from interior bottle to outer bottle or outer bottle to the conduction of its inside material of realization that the bottle can both be as fast as possible to interior bottle through setting up the form that runs through from top to bottom to, and through the mode that slope and non-equipartition set up, can so that at the stirring in-process, inside and outside can both form good whirl state, improves rotatory effect.

Preferably, the strip-shaped holes are arranged in a non-uniform distribution manner.

Preferably, the mouth of the inner bottle is hermetically sleeved on the mouth of the outer bottle, the vacuumizing mechanism comprises a bottle plug clamped on the mouth of the inner bottle, a delivery pipe is hermetically inserted on the bottle plug, and the delivery pipe is communicated with a vacuum pump.

Preferably, the bottle stopper comprises a column body arranged at the middle part, an annular sealing ring is connected to the periphery of the column body, a gap for clamping the inner bottle and the outer bottle is arranged between the annular sealing ring and the column body, and the annular sealing ring is hermetically sleeved on the outer side of the bottle mouth of the outer bottle. This application sets up the bottle plug into including cylinder and annular sealing ring two parts, and the cylinder can realize the sealed of interior bottle, because sealed only adopts ground glass sealed between interior bottle and the outer bottle, sealed effect is effective, when the negative pressure appears in inside, very easily takes place gas leakage phenomenon, and annular sealing ring then can avoid the emergence of this kind of phenomenon, guarantees the complete sealing here in basically.

Preferably, the bottle stopper is made of silicon rubber.

Preferably, the outer bottle comprises a bottle bottom of a substantially planar form; the stirring mechanism is a magnetic stirrer and comprises a rotor arranged at the bottom of the outer bottle and a stirring base matched with the rotor.

Preferably, a sealing rubber plug is arranged at the outlet of the discharge pipe. When having the agrobacterium solution inside, the effect that sealed rubber buffer played is mainly sealed the agrobacterium solution wherein, when the agrobacterium solution is discharged, then can play and take out unnecessary moisture time, guarantees the effect of inside negative pressure stability.

Preferably, a waste liquid collecting vessel is arranged at the outlet of the discharge pipe in a matching manner, and a vessel cover is arranged on the waste liquid collecting vessel in a matching manner.

Preferably, a timer is further provided on the stirring mechanism.

This application can bring following beneficial effect:

1. the plant material can be fully impregnated, the conversion efficiency is improved, the discharge pipe arranged at the bottom can pump out the redundant moisture in the bottle body, so that the mixed bacteria pollution is reduced, and the vacuumizing device arranged at the top can remove the redundant moisture in the bottle body as fast as possible, so that the mixed bacteria pollution is avoided;

2. the conditional hole of this application can be so that whether from interior bottle to outer bottle or outer bottle to the conduction of its inside material of realization that the bottle can both be as fast as possible to interior bottle through setting up the form that runs through from top to bottom to, and through the mode that slope and non-equipartition set up, can so that at the stirring in-process, inside and outside can both form good whirl state, improves rotatory effect.

3. This application sets up the bottle plug into including cylinder and annular sealing ring two parts, and the cylinder can realize the sealed of interior bottle, because sealed only adopts ground glass sealed between interior bottle and the outer bottle, sealed effect is effective, when the negative pressure appears in inside, very easily takes place gas leakage phenomenon, and annular sealing ring then can avoid the emergence of this kind of phenomenon, guarantees the complete sealing here in basically.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a schematic structural diagram of the present application;

FIG. 2 is a schematic structural view of an inner bottle;

fig. 3 is a schematic structural view of the bottle stopper.

Detailed Description

In order to clearly explain the technical features of the present invention, the present application will be explained in detail by the following embodiments in combination with the accompanying drawings.

As shown in the drawings, the following detailed description is given by way of example in order to more clearly explain the overall concept of the present application.

In addition, in the description of the present application, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", and the like, indicate orientations and positional relationships based on those shown in the drawings, are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present application.

The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; the connection can be mechanical connection, electrical connection or communication; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. In the description herein, reference to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

In a first embodiment, as shown in fig. 1-3, an improved device for agrobacteria infection plant callus comprises an outer bottle 1 containing agrobacteria solution, an inner bottle 2 sleeved in the outer bottle 1, a plurality of through holes arranged on the inner bottle 2, and a discharge pipe 13 arranged at the bottom of the outer bottle 1; the vacuum bottle further comprises a vacuum pumping mechanism 3, wherein the vacuum pumping mechanism 3 is arranged at the bottleneck of the inner bottle 2; the outer bottle 1 is also internally provided with a stirring mechanism 4 for driving the agrobacterium solution in the outer bottle 1 to rotate. Interior bottle 2 includes cylindrical bottle, the closed setting in bottom of interior bottle 2, the lateral part of interior bottle 2 is equipped with a plurality of ways and runs through the bar hole 5 that sets up from the top down in order to form the through-hole. The strip-shaped holes 5 are obliquely arranged, and the oblique direction of the strip-shaped holes 5 is consistent with the direction in which the stirring mechanism 4 drives the agrobacterium solution to rotate; the included angle between the axis of the strip-shaped hole 5 and the horizontal direction is not less than 60 degrees, and the width of the strip-shaped hole 5 is not more than 1 mm. The strip-shaped holes 5 are arranged in a non-uniform distribution manner. The bottle mouth of the inner bottle 2 is hermetically sleeved on the bottle mouth of the outer bottle 1, the vacuumizing mechanism 3 comprises a bottle plug 6 clamped on the bottle mouth of the inner bottle 2, a delivery pipe 7 is hermetically inserted on the bottle plug 6, and the delivery pipe 7 is communicated with a vacuum pump 8. The bottle stopper 6 comprises a column body 9 arranged in the middle, an annular sealing ring 10 is connected to the periphery of the column body 9, a gap 11 used for clamping the inner bottle 2 and the outer bottle 1 is arranged between the annular sealing ring 10 and the column body 9, and the annular sealing ring 10 is hermetically sleeved on the outer side of the bottle opening of the outer bottle 1. The bottle stopper 6 is made of silicon rubber material. The outer bottle 1 comprises a bottle bottom in a substantially planar form; rabbling mechanism 4 is magnetic stirrers, rabbling mechanism 4 is including establishing rotor 11 and the stirring base 12 that sets up with rotor 11 cooperation in outer bottle 1 bottom. A sealing rubber plug 14 is arranged at the outlet of the discharge pipe 13; a waste liquid collecting dish 15 is arranged at the outlet of the discharge pipe 13 in a matching way, and a dish cover is arranged on the waste liquid collecting dish 15. A timer 16 is also provided on the stirring mechanism 4.

When in use, plant materials with good growth and no browning are selected as a dip-dyeing receptor; shaking the agrobacterium to be used as a dip dyeing solution; putting plant materials into the inner bottle 2, and pouring the dip dyeing liquid into the outer bottle 1; turning on the stirrer mechanism, setting time through the timer 16, and carrying out dip dyeing; after the dip dyeing is finished, opening a sealing rubber plug 14 on a discharge pipe 13 below, draining the dip dyeing liquid to a waste liquid collecting vessel 15, and covering a vessel cover after the collection is finished; a vacuumizing mechanism 3 connected with the bottle stopper 6 is used for maintaining the negative pressure state of the inner bottle 2 and the outer bottle 1 and discharging excessive moisture as soon as possible; when the corresponding requirements are met, the bottle stopper 6 is opened, and the plant material in the inner bottle 2 is transferred to the culture medium.

The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the system embodiment, since it is substantially similar to the method embodiment, the description is simple, and for the relevant points, reference may be made to the partial description of the method embodiment.

The above are merely examples of the present application and are not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims (10)

1. An improvement device that agrobacterium infects plant callus which characterized in that: the device comprises an outer bottle for containing an agrobacterium solution, an inner bottle is sleeved in the outer bottle, a plurality of through holes are arranged on the inner bottle, and a discharge pipe is arranged at the bottom of the outer bottle; the vacuum-pumping mechanism is arranged at the bottleneck of the inner bottle; and a stirring mechanism for driving the agrobacterium solution in the outer bottle to rotate is also arranged in the outer bottle.

2. The improved device for agrobacterium-infected plant callus according to claim 1, wherein: the inner bottle comprises a cylindrical bottle body, the bottom of the inner bottle is closed, and a plurality of strip-shaped holes penetrating through the inner bottle from top to bottom are formed in the side portion of the inner bottle to form through holes.

3. The improved device for agrobacterium-infected plant callus as claimed in claim 2, wherein: the strip-shaped holes are obliquely arranged, and the oblique direction of the strip-shaped holes is consistent with the direction of the agrobacterium solution driven by the stirring mechanism to rotate; the included angle between the axis of the strip-shaped hole and the horizontal direction is not less than 60 degrees, and the width of the strip-shaped hole is not more than 1 mm.

4. The improved device for agrobacterium-infected plant callus as claimed in claim 2, wherein: the strip-shaped holes are arranged in a non-uniform distribution mode.

5. The improved device for agrobacterium-infected plant callus according to claim 1, wherein: the vacuum pumping mechanism comprises a bottle plug clamped on the bottle mouth of the inner bottle, a delivery pipe is inserted on the bottle plug in a sealing manner, and the delivery pipe is communicated with a vacuum pump.

6. The improved device for agrobacterium-infected plant callus according to claim 5, wherein: the bottle stopper comprises a column body arranged at the middle part, an annular sealing ring is connected at the periphery of the column body, a gap for clamping the inner bottle and the outer bottle is arranged between the annular sealing ring and the column body, and the annular sealing ring is hermetically sleeved at the outer side of the bottle mouth of the outer bottle.

7. The improved device for agrobacterium-infected plant callus according to claim 6, wherein: the bottle stopper is made of silicon rubber.

8. The improved device for agrobacterium-infected plant callus according to claim 1, wherein: said outer bottle including a substantially planar form bottom; the stirring mechanism is a magnetic stirrer and comprises a rotor arranged at the bottom of the outer bottle and a stirring base matched with the rotor.

9. The improved device for agrobacterium-infected plant callus according to claim 1, wherein: a sealing rubber plug is arranged at the outlet of the discharge pipe; and a waste liquid collecting vessel is arranged at the outlet of the discharge pipe in a matching manner, and a vessel cover is arranged on the waste liquid collecting vessel in a matching manner.

10. The improved device for agrobacterium-infected plant callus according to claim 9, wherein: a timer is also arranged on the stirring mechanism.

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