CN214458055U - A culture plate device for living cell is observed - Google Patents

Abstract

The present disclosure provides a culture plate device for live cell observation, comprising: the culture device comprises a bottom plate, a shell, a partition plate, a culture bin and an isolation layer; the shell is fixedly connected with the bottom plate and forms an accommodating space with an open end with the bottom plate in an enclosing manner; the partition board is arranged in the accommodating space and divides the accommodating space into a liquid adding groove positioned at the far end and an accommodating groove positioned at the near end; the isolation layer is laid in the containing groove and divides the containing groove into an upper layer space and a lower layer space; a groove path for guiding waste liquid is formed between the peripheral surface of the culture bin and the inner wall of the shell in the upper layer space; the culture bin comprises a plurality of culture tanks; the culture tanks are arranged in parallel and a gap is arranged between the culture tanks; the culture tank penetrates through the isolation layer; the gap is divided into an upper gap and a lower gap corresponding to the upper layer space and the lower layer space through the isolation layer; the culture tank comprises a plurality of culture holes which are arranged in sequence; a passage is formed among the liquid feeding groove, the lower gap, the culture hole and the groove path; through the utility model discloses an embodiment can carry out half the operation of trading liquid, trading liquid and trading liquid in succession entirely.

Description

A culture plate device for living cell is observed

Technical Field

The utility model belongs to the field of biological instruments; and more particularly, to a culture plate device for living cell observation.

Background

At present, the operation methods of full liquid change and half liquid change exist in the cell culture mode, and the full liquid change is to pour or suck out the culture medium in the original culture vessel and add fresh culture medium. The half-replacement solution is to suck out part of the culture medium in the original culture vessel and then add half of the fresh culture medium. In the course of cell culture, in particular, the culture of nerve cells or brain-like organs usually requires a half-exchange rather than a complete exchange for culturing ordinary cells. As only half liquid replacement is needed, longer time is inevitably needed for liquid replacement under the condition of the traditional culture equipment, a large amount of manpower is wasted, and the process of test monitoring is more time-consuming. How to synchronously complete the half-liquid-changing culture brings huge challenges to the traditional culture equipment.

In addition, because there is a difference in evaporation efficiency between the peripheral and central culture wells of the existing culture plate device for living cell observation, test data of the outermost culture well is often discarded in experiments, and thus, a great waste is caused.

Therefore, there is an urgent need to develop a culture plate device for living cell observation that can achieve both synchronization and half-exchange of liquid and can perform both full-exchange and continuous-exchange of liquid.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned problem that exists among the prior art, the utility model provides a simple structure, convenient operation and can implement in step and partly trade liquid and compromise the culture plate device that is used for living cell observation of trading liquid entirely.

In order to achieve the above object, the embodiment of the present invention adopts the following technical solutions:

there is provided a culture plate device for living cell observation, comprising: the culture device comprises a bottom plate, a shell, a partition plate, a culture bin and an isolation layer; the shell is fixedly connected to the bottom plate, and forms an accommodating space with an open end with the bottom plate in an enclosing manner; the partition board is arranged in the accommodating space and divides the accommodating space into a liquid adding groove at the far end and an accommodating groove at the near end; the isolation layer is laid in the accommodating groove and divides the accommodating groove into an upper layer space and a lower layer space; the culture bin is fixedly arranged in the accommodating groove and one side of the culture bin is attached to the partition plate; a groove path for guiding waste liquid is formed between the peripheral surface of the culture bin and the inner wall of the shell in the upper layer space; the culture bin comprises a plurality of culture tanks; the culture tanks are arranged in parallel and a gap is arranged between the culture tanks; the culture tank penetrates through the isolation layer; the bottom of the culture tank is fixedly arranged on the bottom plate; the gap is divided into an upper gap and a lower gap corresponding to the upper layer space and the lower layer space through the isolation layer, wherein the upper gap is communicated with the tank way, and the lower gap is communicated with the liquid adding tank; the culture tank comprises a plurality of culture holes which are arranged in sequence; an upper opening and a lower opening are arranged on the side wall of the culture hole; each culture well is communicated with the tank circuit through an upper opening thereof and the upper gap, and each culture well is communicated with the liquid feeding tank through a lower opening thereof and the lower gap; and a passage is formed among the liquid adding tank, the lower gap, the culture hole, the upper gap and the channel, so that the culture liquid flows into the lower gap after entering the liquid adding tank, enters the culture hole through the lower opening, then flows out through the upper opening and flows into the channel through the upper gap.

In some embodiments of the present disclosure, the upper opening and the lower opening are both disposed on a sidewall of one side of the culture well.

In some embodiments of the present disclosure, the culture plate device for live cell observation further comprises a blocking mechanism comprising a slide and a closure door; the slideway corresponds to the culture hole and is fixedly arranged on the bottom plate, and the closed door is slidably arranged on the slideway corresponding to the lower opening.

In some embodiments of the present disclosure, the closing door is constructed in a structure in which a magnetic plate is covered with plastic to be opened or closed by magnetic force manipulation.

In some embodiments of the present disclosure, the culture compartment comprises 12 culture tanks arranged in parallel; the culture tank is divided into 8 culture wells.

In some embodiments of the present disclosure, the culture well is configured as an elongated shape, and the inner corners of the culture well are both configured as an arc shape.

In some embodiments of the present disclosure, the insulation layer is configured to extend from a distal end to a proximal end toward the base plate and slope downward.

In some embodiments of the present disclosure, the distal end of the insulating layer is correspondingly connected to the middle section of the partition, and the proximal end of the insulating layer is correspondingly connected to the bottom plate.

In some embodiments of the present disclosure, the culture plate device for living cell observation further comprises: a plurality of liquid inlet openings, a plurality of first liquid discharge openings and a plurality of second liquid discharge openings; wherein the liquid inlet opening is arranged at the top of the side wall of the liquid adding groove close to the far end in parallel; the first liquid discharging opening is arranged at the bottom of the side wall of the liquid adding groove close to the far end in parallel; the second liquid discharge opening is juxtaposed at the bottom of the side wall of the channel near the proximal end.

In some embodiments of the present disclosure, the culture plate device for living cell observation further includes an upper cover plate covering the open end to isolate the accommodating space from the outside.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the culture plate device for observing living cells provided by the embodiment of the utility model is characterized in that the side wall of each culture hole is provided with an upper opening and a lower opening, each culture hole is communicated with the tank circuit through the upper opening via the upper gap, and each culture hole is communicated with the liquid adding groove through the lower opening via the lower gap, therefore, each culture hole can be synchronously performed in the process of implementing half liquid replacement; in addition, the requirements of operations such as full liquid change, continuous liquid change and the like can be met.

By providing a closing door in the culture plate device for living cell observation, it is possible to realize that each culture well satisfies the requirement of being isolated from each other, thereby completing the operation of performing different drug treatments on each culture well under isolated conditions. In addition, the closed door can be operated by a magnetic object or device, so that each culture hole can be quickly communicated and isolated with each other and the liquid adding groove or the waste liquid guiding groove.

Because in the embodiment of the utility model provides an in, set up upper shed and under shed on the lateral wall of culture hole, just the under shed with liquid feeding groove intercommunication UNICOM, consequently when carrying out cell culture, all cells have been equivalent to having realized coculture, promptly, every the cell in the culture hole all is in the same culture environment. The condition that 36 holes at the outermost periphery are cut off in the experiment because the evaporation efficiency of the peripheral hole and the central hole is different in the past is effectively avoided. Realize the circulation balance of culture solution and waste liquid, really realize can letting 96 culture holes can all carry out the purpose of cell culture, overcome the difference that leads to because evaporation efficiency is different.

Drawings

In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments generally by way of example and not by way of limitation, and together with the description and claims serve to explain the disclosed embodiments. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

FIG. 1 is a schematic structural view of a culture plate device for living cell observation according to an embodiment of the present invention;

FIG. 2 is a top view of a plate device for live cell observation according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;

FIG. 4 is a cross-sectional view taken along line B-B of FIG. 2;

FIG. 5 is a cross-sectional view taken along line C-C of FIG. 2;

fig. 6 is a sectional view taken in the direction D-D of fig. 2.

Description of the reference numerals

1-a bottom plate; 2-a shell; 3-a separator; 4-culture cabin; 5-an isolating layer;

6-liquid adding tank; 7-a containing groove; 8-groove path; 9-culture tank; 10-upper gap;

11-lower gap; 12-culture well; 13-upper opening; 14-lower opening

21-liquid inlet opening; 22-first drain opening; 23-second drainage opening

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings, but not intended to limit the invention thereto. Embodiments of the present disclosure are described in further detail below with reference to the figures and the detailed description, but the present disclosure is not limited thereto.

All terms (including technical or scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.

Here, it should be noted that the terms "proximal" and "distal" in the present application are both relative to the observer, for example, "proximal" refers to a side close to the observer; conversely, "distal" refers to the side away from the viewer.

In order to realize half liquid change and improve the testing efficiency in the process of cell culture, in particular to the process of culturing nerve cells or brain-like organs, the following design scheme is provided for the purpose.

With reference to fig. 1 to 6, the present invention provides a culture plate device for living cell observation, comprising: the device comprises a bottom plate 1, a shell 2, a partition plate 3, a culture bin 4 and an isolation layer 5; the shell 2 is fixedly connected to the bottom plate 1 and forms an accommodating space with an open end with the bottom plate 1 in an enclosing manner; the partition plate 3 is arranged in the accommodating space and divides the accommodating space into a liquid adding groove 6 positioned at the far end and an accommodating groove 7 positioned at the near end; the isolation layer 5 is laid in the accommodating groove 7 and divides the accommodating groove 7 into an upper layer space and a lower layer space; the culture bin 4 is fixedly arranged in the accommodating groove 7 and is attached to the partition plate 3 at one side of the culture bin; a channel 8 for guiding waste liquid is formed between the outer peripheral surface of the culture bin 4 and the inner wall of the shell 2 in the upper space; the culture chamber 4 includes a plurality of culture tanks 9; the culture tanks 9 are arranged in parallel and a gap is arranged between the culture tanks; the culture tank 9 penetrates through the isolation layer 5; the bottom of the culture tank 9 is fixedly arranged on the bottom plate 1; the gap is divided into an upper gap 10 and a lower gap 11 corresponding to the upper layer space and the lower layer space through an isolation layer 5, wherein the upper gap 10 is communicated with the channel 8, and the lower gap 11 is communicated with the liquid adding groove 6; the culture tank 9 includes a plurality of culture wells 12 arranged in sequence; the side wall of the culture hole 12 is provided with an upper opening 13 and a lower opening 14; each culture well 12 is communicated with the tank passage 8 through an upper opening 13 thereof through an upper gap 10, and each culture well 12 is communicated with the liquid adding tank 6 through a lower opening 14 thereof through a lower gap 11; a passage is formed between the liquid addition tank 6, the lower gap 11, the culture well 12, the upper gap 10 and the channel 8, so that the culture liquid, after entering the liquid addition tank 6, flows into the lower gap 11, enters the culture well 12 through the lower opening 14, then flows out through the upper opening 13, and flows into the channel 8 through the upper gap 10. With the culture plate device for living cell observation of the present embodiment, it is possible to perform half-exchange of liquid while ensuring that each culture well 12 can be synchronized; in addition, the culture plate device for observing living cells also meets the requirements of operations such as liquid full replacement and continuous liquid replacement.

In one embodiment, referring to fig. 3, the upper opening 13 and the lower opening 14 are both disposed on the sidewall of one side of the culture well 12, and by this arrangement, the purpose is to facilitate the production process and improve the efficiency of making the mold. Of course, the upper opening 13 and the lower opening 14 can also be disposed on different sides of the culture well 12, as long as the upper opening 13 and the lower opening 14 are respectively communicated with the corresponding upper gap 10 and the corresponding lower gap 11, and the specific arrangement mode is not further limited herein, and can be adjusted accordingly according to actual requirements.

In one embodiment, the culture plate device for living cell observation further comprises a blocking mechanism comprising a slide (not shown) and a closing door (not shown); the slideway corresponds to the culture hole 12 and is fixedly arranged on the bottom plate 1, and the closed door is slidably arranged on the slideway corresponding to the lower opening 14. In the present embodiment, by closing the door, the lower opening 14 is in a closed or open state, in which the filling is carried out rapidly; in the blocked state, each culture hole 12 can be isolated from the external environment, and after different added nutrients are given, an independent culture solution environment can be maintained, so that each culture hole 12 is respectively positioned in a specific culture solution environment, the growth condition under different culture solution environments can be detected, and the test efficiency is improved.

In one embodiment, the closing door is constructed in a structure in which a magnetic plate is covered with plastic to be opened or closed by magnetic force. In this embodiment, the magnetic plate is usually made of a metal material that can be manipulated by magnetic force, and after the magnetic force is terminated, the magnetic plate loses magnetism, so that the culture solution is in a non-magnetic state. Preferably, the magnetic plate is composed of a nickel material.

In one embodiment, with reference to FIGS. 1 and 2, the incubation well 4 includes 12 incubation tanks 9 arranged side by side; the culture tank 9 is divided into 8 culture holes 12, which is convenient to match with the requirements of relevant equipment in practical application.

In one embodiment, referring to fig. 2, the culture well 12 is formed in a bar shape, and the inner corners of the culture well 12 are formed in an arc shape, so that the culture well can be cleaned after use.

In an embodiment, in combination with fig. 1, 3 and 4, the barrier layer 5 is arranged to extend from the distal end to the proximal end towards the base plate 1 and to slope downwards, so that waste liquid can flow in the same direction for collection or disposal.

In one embodiment, referring to fig. 4, the distal end of the isolation layer 5 is correspondingly connected to the middle section of the partition 3, and the proximal end of the isolation layer 5 is correspondingly connected to the bottom plate 1, so that the waste liquid can be conveniently guided to flow from the distal end to the proximal end, and the waste liquid can be easily cleaned.

In one embodiment, referring to fig. 1, the culture plate device for living cell observation further comprises: a plurality of inlet openings 21, a plurality of first drain openings 22 and a plurality of second drain openings 23; wherein the liquid inlet opening 21 is juxtaposed at the top of the side wall of the liquid adding tank near the distal end; the first liquid discharging opening 22 is arranged at the bottom of the side wall of the liquid adding groove close to the far end in parallel; the second drainage openings 23 are juxtaposed at the bottom of the side walls of the channel near the proximal end. In this embodiment, the feeding tank 6 is connected to the pump through the inlet opening 21 and the first outlet opening 22, respectively, so as to feed the culture solution into the feeding tank 6 and discharge the excess culture solution. In this embodiment, the pump includes a vacuum pump and a peristaltic pump, but is not limited thereto, and may be adjusted accordingly as needed. Correspondingly, the channel 8 communicates with the pump via said second drainage opening 23 for draining the waste liquid. The automatic liquid changing can be realized through the setting mode. In this embodiment, preferably, twelve inlet openings 21 and twelve first outlet openings 22 are provided to allow the culture solution to be relatively uniformly filled into the feeding tank 6 by communicating with the peristaltic pump, so that the observation and recording results are more accurate due to the more uniform distribution of the culture solution.

In one embodiment, the culture plate device for living cell observation further includes an upper cover plate (not shown) covering the open end to isolate the accommodating space from the outside. In this embodiment, the upper cover plate and the housing 2 may be detachably or pivotally connected, and the specific arrangement manner is not limited herein, so as to avoid affecting liquid feeding and liquid replacement.

Moreover, although illustrative embodiments have been described herein, the scope includes any and all embodiments having equivalent elements, modifications, omissions, combinations (e.g., of aspects across various embodiments), adaptations or alterations based on the present disclosure. The elements in the claims are to be interpreted broadly based on the language employed in the claims and not limited to examples described in the specification or during the life of the application. Further, the steps of the disclosed methods may be modified in any manner, including by reordering steps or inserting or deleting steps. It is intended, therefore, that the description be regarded as examples only, with a true scope being indicated by the following claims and their full scope of equivalents.

The above description is intended to be illustrative and not restrictive. For example, the above-described examples (or one or more aspects thereof) may be used in combination with each other. Other embodiments may be utilized, for example, by one of ordinary skill in the art, upon reading the above description. Also, in the foregoing detailed description, various features may be combined together to simplify the present disclosure. This should not be interpreted as intending that an unclaimed disclosed feature is essential to any claim. Thus, the following claims are hereby incorporated into the detailed description as examples or embodiments, with each claim standing on its own as a separate embodiment, and it is contemplated that these embodiments may be combined with each other in various combinations or permutations. The scope of the invention should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.

Claims (10)

1. A culture plate device for live cell observation, comprising: the culture device comprises a bottom plate, a shell, a partition plate, a culture bin and an isolation layer; wherein,

the shell is fixedly connected with the bottom plate and forms an accommodating space with an open end with the bottom plate in an enclosing manner;

the partition board is arranged in the accommodating space and divides the accommodating space into a liquid adding groove at the far end and an accommodating groove at the near end;

the isolation layer is laid in the accommodating groove and divides the accommodating groove into an upper layer space and a lower layer space;

the culture bin is fixedly arranged in the accommodating groove and one side of the culture bin is attached to the partition plate; a groove path for guiding waste liquid is formed between the peripheral surface of the culture bin and the inner wall of the shell in the upper layer space;

the culture bin comprises a plurality of culture tanks; the culture tanks are arranged in parallel and a gap is arranged between the culture tanks; the culture tank penetrates through the isolation layer; the bottom of the culture tank is fixedly arranged on the bottom plate; the gap is divided into an upper gap and a lower gap corresponding to the upper layer space and the lower layer space through the isolation layer, wherein the upper gap is communicated with the tank way, and the lower gap is communicated with the liquid adding tank;

the culture tank comprises a plurality of culture holes which are arranged in sequence; an upper opening and a lower opening are arranged on the side wall of the culture hole; each culture well is communicated with the tank circuit through an upper opening thereof and the upper gap, and each culture well is communicated with the liquid feeding tank through a lower opening thereof and the lower gap;

and a passage is formed among the liquid adding tank, the lower gap, the culture hole, the upper gap and the channel, so that the culture liquid flows into the lower gap after entering the liquid adding tank, enters the culture hole through the lower opening, then flows out through the upper opening and flows into the channel through the upper gap.

2. The culture plate device for observation of living cells according to claim 1, wherein the upper opening and the lower opening are provided on a side wall of one side of the culture well.

3. A culture plate device for live cell observation according to claim 1, further comprising a blocking mechanism comprising a slide and a closing door; the slideway corresponds to the culture hole and is fixedly arranged on the bottom plate, and the closed door is slidably arranged on the slideway corresponding to the lower opening.

4. The culture plate device for observation of living cells according to claim 3, wherein the closing door is constructed in a structure in which a magnetic plate is covered with plastic to be opened or closed by magnetic force manipulation.

5. The culture plate device for observation of living cells according to claim 3, wherein the culture compartment comprises 12 culture tanks arranged in parallel; the culture tank is divided into 8 culture wells.

6. A culture plate device for living cell observation according to claim 5, wherein the culture well is provided in a long bar shape, and inner corners of the culture well are each provided in an arc shape.

7. The culture plate device for observation of living cells of claim 1, wherein the isolation layer is provided to extend from a distal end to a proximal end toward the bottom plate and to be inclined downward.

8. The culture plate device for observation of living cells of claim 7, wherein a distal end of the isolation layer is correspondingly connected to the middle section of the partition plate, and a proximal end of the isolation layer is correspondingly connected to the bottom plate.

9. The culture plate device for observation of living cells according to claim 8, further comprising, provided on the side walls of the housing, respectively: a plurality of liquid inlet openings, a plurality of first liquid discharge openings and a plurality of second liquid discharge openings; wherein,

the liquid inlet opening is arranged at the top of the side wall of the liquid adding groove close to the far end in parallel;

the first liquid discharging opening is arranged at the bottom of the side wall of the liquid adding groove close to the far end in parallel;

the second liquid discharge opening is juxtaposed at the bottom of the side wall of the channel near the proximal end.

10. A culture plate device for living cell observation according to any one of claims 1 to 9, further comprising an upper cover plate which is provided on the open end so as to cover the accommodation space from the outside.

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