CN214416702U

CN214416702U - Purification equipment is used in antibody production

Info

Publication number: CN214416702U
Application number: CN202023153978.1U
Authority: CN
Inventors: 唐率钧; 李乃鹏; 霍苏科
Original assignee: Qingdao Zhongren Aolan Bioengineering Co Ltd
Current assignee: Qingdao Zhongren Aolan Bioengineering Co Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2021-10-19
Anticipated expiration: 2030-12-24

Abstract

The utility model discloses a purification equipment for antibody production, which comprises a box body and handles, wherein the handles are installed at two ends of the box body, one end of the box body is provided with an observation window, transparent glass is embedded in the observation window, the upper end of the box body is rotatably connected with an upper cover, one end of the upper cover is fixed with a fastener, the upper cover is fixedly connected with the box body through the fastener, a fixed motor is fixed in the box body, an air pump is fixed at the upper end of the fixed motor, the output end of the fixed motor is fixedly connected with the output end of the air pump, a rotating plate is fixed at the output end of the air pump, the purification equipment adopts a left fixed plate and a right fixed plate which are in sliding fit, antibody test tubes put in batches can be sleeved in the two fixed plates, the fixed plates and the antibody test tubes can be taken out from the interior of the centrifugal equipment together after centrifugal treatment is finished, thus, one antibody test tube is not needed to be taken out, greatly quickening the efficiency of centrifugal treatment and being more convenient to use.

Description

Purification equipment is used in antibody production

Technical Field

The utility model belongs to the technical field of the antibody engineering is relevant, concretely relates to purification equipment is used in antibody production.

Background

Antibody engineering refers to engineering of using recombinant DNA and protein engineering techniques to process, modify and reassemble antibody genes, transfecting appropriate receptor cells, expressing antibody molecules, or modifying antibody molecules by cell fusion, chemical modification, etc., as described in patent nos.: the centrifugal device for purifying antibodies proposed in CN211538229U can purify antibodies in batches and is relatively simple in operation, but there is a problem that the antibodies purified in batches are inconvenient to take inside the centrifugal device, and can be taken one by one, so that it is very troublesome to put the antibodies before centrifugation or take the antibodies after centrifugation, which affects the efficiency of antibody purification.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an antibody production is with purification equipment to can not a plurality ofly take the problem that influences the purification availability factor from centrifugal equipment inside when solving the centrifugal purification in batches of the antibody that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a purification device for antibody production comprises a box body and handles, wherein the handles are installed at two ends of the box body, an observation window is formed in one end of the box body, transparent glass is embedded in the observation window, an upper cover is rotatably connected to the upper end of the box body, a fastener is fixed to one end of the upper cover, the upper cover is fixedly connected with the box body through the fastener, a fixed motor is fixed to the interior of the box body, an air pump is fixed to the upper end of the fixed motor, the output end of the fixed motor is fixedly connected with the output end of the air pump, a rotating plate is fixed to the output end of the air pump, a supporting rod is fixed to the upper end of the rotating plate, a bottom plate is fixed to the upper end of the supporting rod, an alignment port is formed in the upper end of the bottom plate, a left fixed plate is fixed to the upper end of the bottom plate, a fixed column is fixed to the lower end of the left fixed plate, a notch corresponding to the fixed column is formed in the upper end of the bottom plate, the one end sliding connection of left side fixed plate has right fixed plate, the one end of left side fixed plate is fixed with the counterpoint post, the opening that corresponds with the counterpoint post is seted up to the one end of right side fixed plate, the spread groove has been seted up to the upper end of right side fixed plate and left fixed plate, fixed motor and external power source electric connection.

Preferably, the upper end of the box body is fixedly connected with a hinge, the other end of the hinge is fixedly connected with the upper cover, and one end of the upper cover is rotatably connected with the box body through the hinge.

Preferably, the connecting groove is arranged at the upper end of the alignment port, and the width of the connecting groove is the same as the diameter of the alignment port.

Preferably, the outer walls of the left fixing plate and the bottom plate can be completely attached, and the length of the left fixing plate is greater than half of that of the bottom plate.

Preferably, the alignment ports are arranged in twelve numbers, and twelve alignment ports are arranged in the upper end of the bottom plate at equal intervals.

Preferably, the extension length of the air pump is less than the distance from the left fixing plate to the outer wall of the lower end of the upper cover.

Compared with the prior art, the utility model provides an antibody production is with purification equipment possesses following beneficial effect:

one, this novel porous bottom plate of adoption for with the antibody test tube batch centrifugal treatment that needs centrifugal treatment, make single centrifugal treatment's antibody volume more, can make full use of centrifugal equipment for the efficiency of purification processing.

Two, this novel adoption sliding fit's left fixed plate and right fixed plate can cup joint the antibody test tube of putting into in batches inside two fixed plates, can together take out from centrifugal equipment inside with fixed plate intercommunication antibody test tube after centrifugal treatment accomplishes, just so need not an antibody test tube of taking for centrifugal treatment's efficiency greatly, and it is more convenient to use.

Thirdly, this novel adoption air pump and fixed motor cooperation make the antibody test tube centrifugation fast, and the fixed motor rotates the test tube and makes the antibody centrifugation, and the air pump can shake about the jacking test tube for centrifugation's efficiency makes this novel purification centrifugal equipment use more convenient.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description, do not constitute a limitation of the invention, in which:

FIG. 1 is a schematic view of a three-dimensional structure of a purification apparatus for antibody production according to the present invention;

FIG. 2 is a schematic diagram of a front cross-sectional structure of a purification apparatus for antibody production according to the present invention;

FIG. 3 is a schematic view of a top cross-sectional structure of a purification apparatus for antibody production according to the present invention;

fig. 4 is the utility model provides a left side fixed plate and right fixed plate front sectional structure sketch map.

In the figure: 1. transparent glass; 2. an observation window; 3. a fastener; 4. an upper cover; 5. a box body; 6. a handle; 7. a left fixing plate; 8. a right fixing plate; 9. a base plate; 10. a support bar; 11. rotating the plate; 12. an air pump; 13. fixing a motor; 14. connecting grooves; 15. aligning ports; 16. fixing a column; 17. and (4) aligning columns.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a purification equipment is used in antibody production, including left fixed plate 7, right fixed plate 8, box 5, bottom plate 9, air pump 12, fixed motor 13, it is fixed with the antibody test tube that needs centrifugal treatment through counterpoint mouth 15 on the bottom plate 9, it is centrifugal to drive bottom plate 9 through fixed

motor

13, 12 jacking bottom plates of air pump 9 reciprocate simultaneously and accelerate centrifugal efficiency, take off left fixed plate 7 and right fixed plate 8 together with the test tube from bottom plate 9 upper end after centrifugal treatment accomplishes, put into the test-tube rack inside with the test tube, stretch out right fixed plate 8 from 7 one end of left fixed plate again and can take out all test tubes in the lump.

Handles 6 are installed at two ends of a box body 5, an observation window 2 is arranged at one end of the box body 5, transparent glass 1 is embedded in the observation window 2, an upper cover 4 is rotatably connected at the upper end of the box body 5, a fastener 3 is fixed at one end of the upper cover 4, the upper cover 4 is fixedly connected with the box body 5 through the fastener 3, a fixed motor 13 is fixed in the box body 5, an air pump 12 is fixed at the upper end of the fixed motor 13, the output end of the fixed motor 13 is fixedly connected with the output end of the air pump 12, a rotating plate 11 is fixed at the output end of the air pump 12, a support rod 10 is fixed at the upper end of the rotating plate 11, a bottom plate 9 is fixed at the upper end of the support rod 10, an alignment port 15 is arranged at the upper end of the bottom plate 9, a left fixing plate 7 is fixed at the upper end of the bottom plate 7, a fixing column 16 is fixed at the lower end of the left fixing plate 7, a notch corresponding to the fixing column 16 is arranged at the upper end of the bottom plate 9, a right fixing plate 8 is slidably connected with the left fixing plate 7, the one end of left fixed plate 7 is fixed with counterpoint post 17, and the opening that corresponds with counterpoint post 17 has been seted up to the one end of right fixed plate 8, and spread groove 14, fixed motor 13 and external power source electric connection have been seted up to the upper end of right fixed plate 8 and left fixed plate 7.

In order to rotate the upper cover 4 conveniently, the upper end of the box body 5 is fixedly connected with a hinge, the other end of the hinge is fixedly connected with the upper cover 4, and one end of the upper cover 4 is rotatably connected with the box body 5 through the hinge, so that the upper cover 4 can be opened from the upper end of the box body 5 conveniently.

In order to guarantee fixed effect, connecting groove 14 sets up in the upper end to mouthful 15, and the width of connecting groove 14 is the same with the diameter size of counterpointing mouthful 15 for the test tube that holds the antibody is put into and is counterpointed mouthful 15 inside also can with the laminating of connecting groove 14 inner wall, and the reduction is rocked.

For the convenience of taking off, the outer wall of left fixed plate 7 and bottom plate 9 can laminate completely, and the length of left fixed plate 7 is greater than half of bottom plate 9 length, can follow both ends very conveniently like this and take off left fixed plate 7 and right fixed plate 8 from bottom plate 9 upper end.

For batch processing, the aligning ports 15 are arranged in twelve, twelve aligning ports 15 are equidistantly arranged in the upper end of the bottom plate 9, so that the number of the antibody test tubes subjected to single centrifugal processing is larger, and the centrifugal purification efficiency is higher.

In order to prevent the test tube from touching the upper cover 4, the extension length of the air pump 12 is less than the distance from the left fixing plate 7 to the outer wall of the lower end of the upper cover 4, so that the fixing plate can move up and down along with the bottom plate 9 at the output end of the air pump 12 and can not contact with the inner wall of the upper cover 4.

The working principle is as follows: the novel purification equipment for antibody production adopts the fixed motor 13 and the air pump 12 to carry out high-efficiency centrifugal treatment on antibody test tubes, and can conveniently take the test tubes from the inside of the centrifugal equipment in batches through the two fixing plates which are in sliding connection, when in use, the upper cover 4 is opened through the fastener 3, the antibody test tubes needing centrifugal treatment are put into the bottom plate 9 through the alignment port 15, the test tubes can be centrifugally treated by closing the upper cover 4 and starting the fixed motor 13 and the air pump 12, after treatment is finished, the upper cover 4 is opened, the left fixing plate 7 and the right fixing plate 8 are taken out together by communicating the test tubes from the upper end of the bottom plate 9, the taken test tubes are aligned with the test tube rack and fixed, the left fixing plate 7 and the right fixing plate 8 are stretched towards the two ends so that the alignment column 17 is pulled out from the inside of the right fixing plate 8, the two fixing plates can be taken out from the outside of the test tubes through the connecting groove 14, after taking out, the two fixing plates are sleeved together through the alignment column 17, the upper end of the bottom plate 9 is fixed in an aligned mode through the fixing columns 16 and then is used centrifugally.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A purification equipment for antibody production, includes box (5) and handle (6), its characterized in that: handles (6) are installed at two ends of the box body (5), an observation window (2) is arranged at one end of the box body (5), transparent glass (1) is embedded in the observation window (2), an upper cover (4) is rotatably connected to the upper end of the box body (5), a fastener (3) is fixed to one end of the upper cover (4), the upper cover (4) and the box body (5) are fixedly connected through the fastener (3), a fixed motor (13) is fixed to the inside of the box body (5), an air pump (12) is fixed to the upper end of the fixed motor (13), the output end of the fixed motor (13) is fixedly connected with the output end of the air pump (12), a rotating plate (11) is fixed to the output end of the air pump (12), a support rod (10) is fixed to the upper end of the rotating plate (11), a bottom plate (9) is fixed to the upper end of the support rod (10), a position aligning port (15) is formed in the upper end of the bottom plate (9), the upper end of bottom plate (9) is fixed with left fixed plate (7), the lower extreme of left fixed plate (7) is fixed with fixed column (16), the notch that corresponds with fixed column (16) is seted up to the upper end of bottom plate (9), the one end sliding connection of left fixed plate (7) has right fixed plate (8), the one end of left fixed plate (7) is fixed with counterpoint post (17), the opening that corresponds with counterpoint post (17) is seted up to the one end of right fixed plate (8), spread groove (14) have been seted up to the upper end of right fixed plate (8) and left fixed plate (7), fixed motor (13) and external power source electric connection.

2. The purification apparatus for antibody production according to claim 1, wherein: the upper end fixedly connected with hinge of box (5), the other end and upper cover (4) fixed connection of hinge, the one end of upper cover (4) is passed through the hinge and is rotated with box (5) and be connected.

3. The purification apparatus for antibody production according to claim 1, wherein: the connecting groove (14) is arranged at the upper end of the alignment opening (15), and the width of the connecting groove (14) is the same as the diameter of the alignment opening (15).

4. The purification apparatus for antibody production according to claim 1, wherein: the outer walls of the left fixing plate (7) and the bottom plate (9) can be completely attached, and the length of the left fixing plate (7) is larger than half of that of the bottom plate (9).

5. The purification apparatus for antibody production according to claim 1, wherein: the alignment ports (15) are arranged in twelve numbers, and twelve alignment ports (15) are arranged in the upper end of the bottom plate (9) at equal intervals.

6. The purification apparatus for antibody production according to claim 1, wherein: the extension length of the air pump (12) is less than the distance from the left fixing plate (7) to the outer wall of the lower end of the upper cover (4).