CN111040929A

CN111040929A - Gene capturing kit for capturing related genes of hematopathy

Info

Publication number: CN111040929A
Application number: CN201911404946.3A
Authority: CN
Inventors: 刘俊玲; 田猛
Original assignee: QINGDAO HISER MEDICAL CENTER
Current assignee: QINGDAO HISER MEDICAL CENTER
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-04-21

Abstract

The invention discloses a gene capturing kit for capturing genes related to blood diseases, which comprises a base, wherein the top of the base is fixedly connected with a box body, the lower surface of the box body is fixedly connected with a motor, the top of an output shaft on the motor is fixedly connected with a rotating shaft, the surface of the rotating shaft penetrates through the bottom of the box body and is rotatably connected with the box body, the top of the rotating shaft is fixedly connected with a first disk, the upper surface of the first disk is fixedly connected with an arc-shaped rack, and the inner wall of the box body is limited and rotated by a transmission shaft. According to the invention, through the matching use of the structures, the problems that in the actual use process, as the function of the traditional gene capture kit is single, when a sample captured by the gene needs to be centrifuged and shaken, the sample needs to be continuously transferred, the operation is tedious, time-consuming and labor-consuming, and finally the sample is easily placed and misplaced when the sample is transferred into the kit, so that the inconvenience is brought to the use, and the working efficiency is reduced are solved.

Description

Gene capturing kit for capturing related genes of hematopathy

Technical Field

The invention relates to the technical field of gene capturing equipment, in particular to a gene capturing kit for capturing related genes of blood diseases.

Background

The gene capture technology is one of the most promising gene cloning methods at present. The random insertion mutation mutant library established by the technology can be used for searching, identifying and researching a large number of unknown functions and known function activated genes, and is a novel molecular biological method developed after natural mutation, physical mutation and chemical mutation.

The gene capture vector is an incomplete gene expression vector with a reporter gene and/or a selectable marker gene; the genes carried by these vectors can only be expressed when they are integrated into the functional host genes and are in-frame with the fused host genes; gene trapping is commonly used for researches in Mendelian diseases such as hematopathy, protooncogenes, cancer suppressor genes, recessive non-syndromic deafness, severe craniocerebral deformity, geisha syndrome and the like; in order to cooperate with the gene capture research, the traditional kit is used for storing samples, but the traditional kit compares each drug independently, so that the detection time is too long; and the experimental result is influenced due to different external environmental factors; meanwhile, the structure is single, the detection time needs to be recorded manually, the error is large, a gene capture kit is provided for the people, for example, the gene capture kit for capturing related genes of the blood diseases is disclosed in Chinese patent CN109633177A, and a contrast vessel is arranged on the right side of the shell; the antibodies of the related blood disease proteins are paired antibodies, and each pair of antibodies respectively corresponds to a detection index. The gene capture kit provided by the invention has the advantages of simple structure, reasonable design, simple and convenient capture and higher sensitivity;

however, in the actual use process, because the function of the traditional gene capturing kit is single, when the sample captured by the gene needs to be centrifuged and shaken, the sample needs to be continuously transferred, which is tedious, time-consuming and labor-consuming, and finally, when the sample is transferred into the kit, the sample is easily placed and misplaced, which brings inconvenience to the use and reduces the working efficiency.

Disclosure of Invention

The invention aims to provide a gene capturing kit for capturing genes related to blood diseases, which improves the traditional device and solves the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a gene capture kit for catching blood disease related gene, includes the base, the top fixedly connected with box body of base, the lower fixed surface of box body is connected with the motor, the top fixedly connected with pivot of output shaft on the motor, the surface of pivot runs through the bottom of box body and rotates with the box body to be connected, the top fixedly connected with disc one of pivot.

The last fixed surface of disc one is connected with the arc rack, the spacing rotation of inner wall of box body has the transmission shaft, two gears one, two of the fixed surface of transmission shaft are connected with one, two the vertical central line symmetry setting of gear one with the pivot, the tooth meshing on the surface of gear one and the arc rack, the fixed surface of transmission shaft is connected with the adapter sleeve, the fixed surface of adapter sleeve is connected with the connecting plate, the top fixedly connected with disc two of connecting plate, the last fixed surface of disc two is connected with the adapter sleeve, the fixed surface of pivot is connected with centrifugal device.

Preferably, centrifugal device includes gear two, the inner wall of gear two is connected with the fixed surface of pivot, the left side meshing of gear two has gear three, the inner wall fixedly connected with axis of rotation of gear three, be equipped with C shape frame in the box body, the axis of rotation runs through C shape frame and spacing rotation in C shape frame, the top fixedly connected with disc three of C shape frame, the draw-in groove has been seted up to the upper surface of disc three.

Preferably, the left side of the C-shaped frame is provided with a threaded hole and is in threaded connection with a screw rod through the threaded hole, and the surface of the screw rod penetrates through the box body and is limited to rotate on the box body.

Preferably, the left end of the screw rod is fixedly connected with a knob, and anti-skid grains are formed in the surface of the knob.

Preferably, the surface of the screw is fixedly connected with two limit rings, and the opposite sides of the two limit rings are respectively connected with the inner wall of the box body and the left side of the box body in a rotating manner.

Preferably, the surface of the motor is fixedly connected with a reinforcing frame, and the top of the reinforcing frame is fixedly connected with the lower surface of the box body.

Preferably, the fastening sleeve is an elastic rubber sleeve, and the inner wall of the fastening sleeve is provided with anti-skid grains.

Compared with the prior art, the invention has the following beneficial effects:

firstly, the base and the box body play a role in fixing and supporting the whole body; when the motor is connected with an external power supply, an output shaft on the motor drives the rotating shaft to synchronously rotate, and the rotating shaft synchronously rotates along with the disk one on the rotating shaft and drives the arc-shaped rack to synchronously rotate through the disk;

when the rotating arc-shaped rack is meshed with the first gear, the first gear is driven to rotate, the transmission shaft on the first gear synchronously rotates along with the first gear, the two first gears are symmetrically arranged along the vertical central line of the rotating shaft, when the arc-shaped rack is meshed with the two first gears in sequence and is driven to rotate, the two gears drive the transmission shaft to rotate in opposite directions in sequence, namely the transmission shaft can rotate in a reciprocating manner, after the arc-shaped rack continuously rotates, the transmission shaft can also rotate in a reciprocating manner in a continuous manner through meshing transmission, the second disc also swings in a reciprocating manner through transmission of the connecting sleeve and the connecting plate, a sample is placed in a capped test tube, and the capped test tube is inserted into the fastening sleeve, so that the test tube is fixed; the shaking of the capped test tube is realized through the swinging of the second disc, and the sample in the capped test tube is also shaken;

the centrifugal device is arranged, so that a sample can be subjected to centrifugal treatment, the functions of the kit are increased, and the integration degree is higher;

through the matching use of the structures, the invention solves the problems that in the actual use process, as the function of the traditional gene capture kit is single, when a sample captured by the gene needs to be subjected to centrifugation and shaking treatment, the sample needs to be continuously transferred, the operation is complicated, time and labor are wasted, and finally the sample is easily placed and misplaced when the sample is transferred into the kit, so that the inconvenience is brought to the use, and the working efficiency is reduced.

Drawings

FIG. 1 is a front cross-sectional view of the structure of the present invention;

FIG. 2 is a top view of a first disk of the present invention.

In the figure: 1-base, 2-box body, 3-motor, 4-rotating shaft, 5-first disk, 6-arc rack, 7-transmission shaft, 8-first gear, 9-connecting sleeve, 10-connecting plate, 11-second disk, 12-fastening sleeve, 13-centrifugal device, 14-second gear, 15-third gear, 16-rotating shaft, 17-C shaped frame, 18-third disk, 19-clamping groove, 20-threaded hole, 21-screw rod, 22-knob, 23-spacing ring and 24-reinforcing frame.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 2, the present invention provides a technical solution: a gene capturing kit for capturing genes related to blood diseases comprises a base 1, wherein the top of the base 1 is fixedly connected with a box body 2, and the base 1 and the box body 2 play a role in integral fixed support; the lower surface of the box body 2 is fixedly connected with the motor 3, after the motor 3 is connected with an external power supply, an output shaft on the motor 3 drives the rotating shaft 4 to synchronously rotate, the surface of the motor 3 is fixedly connected with the reinforcing frame 24, the top of the reinforcing frame 24 is fixedly connected with the lower surface of the box body 2, and the firmness of the motor 3 during installation and use on the box body 2 can be further ensured by adding the reinforcing frame 24 on the motor 3, so that the shaking during use is avoided; the top fixedly connected with pivot 4 of output shaft on the motor 3, the surface of pivot 4 runs through the bottom of box body 2 and rotates with box body 2 to be connected, the top fixedly connected with disc 5 of pivot 4.

The upper surface of the first disc 5 is fixedly connected with an arc-shaped rack 6, and the first disc 5 on the rotating shaft 4 synchronously rotates along with the arc-shaped rack 6 and is driven by the first disc 5 to synchronously rotate; the inner wall of the box body 2 is limited to rotate and provided with a transmission shaft 7, the surface of the transmission shaft 7 is fixedly connected with two first gears 8, the two first gears 8 are symmetrically arranged on the vertical central line of the rotating shaft 4, the surface of the first gear 8 is meshed with teeth on the arc-shaped rack 6, the surface of the transmission shaft 7 is fixedly connected with a connecting sleeve 9, the surface of the connecting sleeve 9 is fixedly connected with a connecting plate 10, the top of the connecting plate 10 is fixedly connected with a second disk 11, when the rotating arc-shaped rack 6 is meshed with the first gears 8, the first gears 8 are driven to rotate, the transmission shaft 7 on the first gears 8 synchronously rotates along with the first gears, because the two first gears 8 are symmetrically arranged on the vertical central line of the rotating shaft 4, when the arc-shaped rack 6 is sequentially meshed with the two first gears 8 and is driven to rotate, the two first gears 8 can sequentially drive the, after the arc-shaped rack 6 continuously rotates, the transmission shaft 7 can also continuously and reciprocally rotate through meshing transmission, the second disc 11 is also reciprocally swung through transmission of the connecting sleeve 9 and the connecting plate 10, a sample is placed in the capped test tube, and the capped test tube is inserted into the fastening sleeve 12 to fix the test tube; the shaking of the capped test tube is realized through the swinging of the second disk 11, and the sample in the capped test tube is also shaken; the upper surface of the second disc 11 is fixedly connected with a fastening sleeve 12, the fastening sleeve 12 is an elastic rubber sleeve, the inner wall of the fastening sleeve is provided with anti-slip lines, the fastening sleeve 12 is an elastic rubber sleeve, the fastening sleeve can be adapted to test tubes with caps with different calibers, and the anti-slip lines on the inner wall of the fastening sleeve can increase friction force, so that the anti-drop effect is improved; the surface of the rotating shaft 4 is fixedly connected with a centrifugal device 13, and the centrifugal device 13 is arranged, so that a sample can be centrifugally treated, the functions of the kit are increased, and the integration degree is higher; the centrifugal device 13 comprises a second gear 14, the inner wall of the second gear 14 is fixedly connected with the surface of the rotating shaft 4, a third gear 15 is meshed on the left side of the second gear 14, a rotating shaft 16 is fixedly connected with the inner wall of the third gear 15, a C-shaped frame 17 is arranged in the box body 2, the rotating shaft 16 penetrates through the C-shaped frame 17 and rotates in the C-shaped frame 17 in a limiting manner, a third disc 18 is fixedly connected to the top of the C-shaped frame 17, a clamping groove 19 is formed in the upper surface of the third disc 18, when the centrifugal device is used, the rotating shaft 4 rotates to drive the second gear 14 to synchronously rotate, the third gear 15 meshed with the second gear 14 drives the rotating shaft 16 therein to rotate, the rotating shaft 16 drives the third disc 18 to rotate through the rotating shaft 16, and before the third disc 18 rotates, a capped test tube filled with a sample; the disc III 18 drives the test tube with the cap to rotate, so that the centrifugal operation of the sample in the test tube is realized; the left side of the C-shaped frame 17 is provided with a threaded hole 20, a screw rod 21 is screwed in the threaded hole 20, the surface of the screw rod 21 penetrates through the box body 2 and rotates on the box body 2 in a limiting mode, the time required by sample centrifugation and shaking operation is different, the time required by the sample centrifugation is shorter than the shaking time, the screw rod 21 and the threaded hole 20 in the C-shaped frame 17 rotate relatively by manually driving the rotation of the screw rod 21, therefore, the C-shaped frame 17 moves left and right in the box body 2, when the C-shaped frame 17 moves right, the gear III 15 in the C-shaped frame 17 is contacted with the left side of the gear II 14, the gear III 15 is completely meshed with the gear II 14 by manually adjusting the angle of the gear III 15, and the gear III 15 is driven to rotate synchronously by the gear II 14; when the centrifugal operation needs to be stopped, the rotation of the output shaft of the motor 3 is stopped firstly, then the screw 21 is rotated reversely, the left movement of the C-shaped frame 17 with the gear III 15 is realized, the gear III 15 is not meshed with the gear II 14, the driving is stopped along with the gear III, then the rotation of the output shaft of the motor 3 is continuously started, so that the sample on the disc II 11 is continuously shaken, the left end of the screw 21 is fixedly connected with the knob 22, the surface of the knob 22 is provided with anti-skid grains, the screw 21 is conveniently driven and rotated by the arrangement of the knob 22 on the screw 21, and the anti-skid grains are arranged on the screw 21, so that the rotation is more labor-saving; the fixed surface of screw rod 21 is connected with two spacing rings 23, and the opposite side of two spacing rings 23 rotates with the inner wall of box body 2 and the left side of box body 2 respectively to be connected, through the setting of two spacing rings 23 on the screw rod 21, can further guarantee the spacing pivoted stability of screw rod 21 in box body 2.

The working principle is as follows: when the gene capturing kit for capturing the related genes of the blood diseases is used, the base 1 and the box body 2 play a role in integral fixing and supporting; when the motor 3 is connected with an external power supply, an output shaft on the motor drives the rotating shaft 4 to synchronously rotate, and the disc I5 on the rotating shaft 4 synchronously rotates along with the rotating shaft, and the disc I5 drives the arc-shaped rack 6 to synchronously rotate; when the rotating arc-shaped rack 6 is meshed with the first gear 8, the first gear 8 is driven to rotate, the transmission shaft 7 on the first gear 8 rotates synchronously with the first gear 8, the two first gears 8 are symmetrically arranged with the vertical central line of the rotating shaft 4, when the arc-shaped rack 6 is meshed with the two first gears 8 successively and is driven to rotate, the two first gears 8 can drive the transmission shaft 7 to rotate in opposite directions successively, namely the transmission shaft 7 can rotate in a reciprocating manner, after the arc-shaped rack 6 rotates continuously, the transmission shaft 7 can also rotate in a reciprocating manner continuously through meshing transmission, the second disk 11 can also swing in a reciprocating manner through transmission of the connecting sleeve 9 and the connecting plate 10, a sample is placed in a test tube with a cap, and then the test tube with the cap is inserted into the fastening sleeve 12, so that the test tube is fixed; the shaking of the capped test tube is realized through the swinging of the second disk 11, and the sample in the capped test tube is also shaken; through the arrangement of the centrifugal device 13, the sample can be centrifugally treated, so that the functions of the kit are increased, and the integration degree is higher; through the cooperation use between the above-mentioned structure, solved in the in-service use process, because traditional gene capture kit's function is comparatively single, when the sample of gene capture need advance the centrifugation and rock the processing, need constantly shift the sample, loaded down with trivial details and waste time and energy, when finally shifting to the kit, still cause placing the dislocation of sample easily, bring inconvenience for the use, reduced work efficiency's problem.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A gene trap kit for trapping a gene associated with a hematological disorder, comprising: the automatic feeding device comprises a base (1), wherein a box body (2) is fixedly connected to the top of the base (1), a motor (3) is fixedly connected to the lower surface of the box body (2), a rotating shaft (4) is fixedly connected to the top of an output shaft on the motor (3), the surface of the rotating shaft (4) penetrates through the bottom of the box body (2) and is rotatably connected with the box body (2), and a first disc (5) is fixedly connected to the top of the rotating shaft (4);

the last fixed surface of disc (5) is connected with arc rack (6), the spacing rotation of inner wall of box body (2) has transmission shaft (7), the fixed surface of transmission shaft (7) is connected with two gear (8), two gear (8) sets up with the vertical central line symmetry of pivot (4), the tooth meshing on the surface of gear (8) and arc rack (6), the fixed surface of transmission shaft (7) is connected with adapter sleeve (9), the fixed surface of adapter sleeve (9) is connected with connecting plate (10), the top fixedly connected with disc two (11) of connecting plate (10), the last fixed surface of disc two (11) is connected with adapter sleeve (12), the fixed surface of pivot (4) is connected with centrifugal device (13).

2. The gene trap kit for trapping a gene associated with a hematological disorder according to claim 1, wherein: centrifugal device (13) are including gear two (14), the inner wall of gear two (14) is connected with the fixed surface of pivot (4), the left side meshing of gear two (14) has gear three (15), the inner wall fixedly connected with axis of rotation (16) of gear three (15), be equipped with C shape frame (17) in box body (2), axis of rotation (16) run through C shape frame (17) and spacing rotation in C shape frame (17), the top fixedly connected with disc three (18) of C shape frame (17), draw-in groove (19) have been seted up to the upper surface of disc three (18).

3. The gene trap kit for trapping a gene related to a hematological disorder according to claim 2, wherein: threaded holes (20) are formed in the left side of the C-shaped frame (17), a screw rod (21) is connected with the threaded holes (20) in a threaded mode, and the surface of the screw rod (21) penetrates through the box body (2) and is limited to rotate on the box body (2).

4. The gene trap kit for trapping a gene associated with a hematological disorder according to claim 3, wherein: the left end fixedly connected with knob (22) of screw rod (21), anti-skidding line has been seted up on the surface of knob (22).

5. The gene trap kit for trapping a gene associated with a hematological disorder according to claim 3, wherein: the surface of the screw rod (21) is fixedly connected with two limiting rings (23), and the opposite sides of the two limiting rings (23) are respectively connected with the inner wall of the box body (2) and the left side of the box body (2) in a rotating mode.

6. The gene trap kit for trapping a gene associated with a hematological disorder according to claim 1, wherein: the surface of the motor (3) is fixedly connected with a reinforcing frame (24), and the top of the reinforcing frame (24) is fixedly connected with the lower surface of the box body (2).

7. The gene trap kit for trapping a gene associated with a hematological disorder according to claim 1, wherein: the fastening sleeve (12) is an elastic rubber sleeve, and the inner wall of the fastening sleeve is provided with anti-skid grains.