CN114308410B - Tumor cell sample pretreatment extraction kit - Google Patents
Tumor cell sample pretreatment extraction kit Download PDFInfo
- Publication number
- CN114308410B CN114308410B CN202111567819.2A CN202111567819A CN114308410B CN 114308410 B CN114308410 B CN 114308410B CN 202111567819 A CN202111567819 A CN 202111567819A CN 114308410 B CN114308410 B CN 114308410B
- Authority
- CN
- China
- Prior art keywords
- metal wire
- wall
- elastic
- strip
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- 239000002184 metal Substances 0.000 claims abstract description 73
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 238000003825 pressing Methods 0.000 claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 57
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract description 22
- 235000017491 Bambusa tulda Nutrition 0.000 abstract description 22
- 241001330002 Bambuseae Species 0.000 abstract description 22
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract description 22
- 239000011425 bamboo Substances 0.000 abstract description 22
- 238000005119 centrifugation Methods 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- 230000009471 action Effects 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000005299 abrasion Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000005266 circulating tumour cell Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Abstract
The invention relates to the technical field of kits, and discloses a tumor cell sample pretreatment extraction kit, which comprises a shell, wherein a receiving disc is rotatably connected to the inner wall of the shell, a metal wire cylinder is rotatably connected to the receiving disc, a centrifugal mechanism is connected to the bottom of the shell through threads, and the centrifugal mechanism comprises a base, a driving gear, a driven gear, a guide connection pipe, an elastic plug rod, a wedge strip, a pressing block, a rotating strip, an elastic sub-pipe and a telescopic supporting rod. This tumour cell sample pretreatment draws kit through with centrifugal mechanism and accepting the design of dish disconnect-type, rotates the metal wire section of thick bamboo of each splendid attire reagent and connects on accepting the dish, controls centrifugal mechanism and metal wire section of thick bamboo zonulae occludens as required, and the cooperation utilizes Bernoulli's principle to realize the contactless type negative suction location to the reagent pipe in the centrifugation process to effectively promoted the practicality that the kit used, high-efficient location convenience and the security of guaranteeing the reagent pipe.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a pretreatment and extraction kit for tumor cell samples.
Background
At present, after tumor cells are extracted in medicine, centrifugal separation operation is needed to be carried out on blood cells doped in the tumor cells, further research experiments such as CTCs detection and the like can be carried out, after conventional tumor cell extraction is completed, the reagent kit is needed to be used for carrying out stable storage of sample extraction, then specific reagent tubes are taken out one by one to other instruments according to the requirements to carry out centrifugal operation, the operation process is complex and troublesome, frequent transfer of the reagent tubes can cause cell activity change caused by factors such as shaking and the like, in addition, when the reagent tubes are centrifuged, operators are needed to carry out contact type extrusion limiting on the reagent tubes by utilizing corresponding fastening and positioning mechanisms, corresponding friction damage is caused on the outer walls of the reagent tubes, and positioning effects obtained by the reagent tubes with different specifications can be different due to fixed-shape fixing mechanisms, so that potential safety hazards appear when equipment is used for handling the reagent tubes with different specifications.
Disclosure of Invention
In order to solve the problems that in the general pretreatment process of tumor cell samples, the practical performance of the kit is low, the positioning during centrifugation of the reagent tube lacks convenience and safety, the practical performance of the kit is effectively improved, and the positioning convenience and safety of the reagent tube are efficiently ensured, the invention is realized by the following technical scheme: the utility model provides a tumour cell sample pretreatment extraction kit, includes the casing, the inner wall of casing rotates to be connected with accepts the dish, it is connected with the metal wire section of thick bamboo to accept to rotate on the dish, the bottom threaded connection of casing has centrifugal mechanism, centrifugal mechanism includes base, drive gear, driven gear, guide tube, elasticity cock stem, wedge, according to piece, commentaries on classics strip, elasticity sub-tube, flexible butt pole, the middle part inner wall of base rotates to be connected with drive gear, the left side of base rotates to be connected with driven gear, the fixed grafting in middle part of driven gear has the guide tube, the bottom slip grafting of guide tube has the elasticity cock stem, the left side inner wall slip grafting of guide tube has the wedge, the left wall slip grafting of guide tube has according to the piece, according to the piece with swing joint has the commentaries on classics strip between the wedge, the top slip connection of guide tube has the elasticity sub-tube, the top inner wall slip connection of elasticity sub-tube has flexible butt pole.
Further, grooves corresponding to the metal wire barrels in number are uniformly formed in the bottom of the bearing disc, and elastic pins extending into the grooves are fixedly connected to the inner wall of the shell, so that the bearing disc is conveniently positioned.
Further, the bottom of the metal wire cylinder comprises a sleeving part corresponding to the telescopic supporting rod, so that the telescopic supporting rod can conveniently extend into the inner cavity of the sleeving part and then can laterally abut against the inner wall of the sleeving part.
Further, the left wall of the elastic plug rod is provided with a wedge groove corresponding to the wedge bar, so that the elastic plug rod can be limited to reset by the wedge bar with an elastic mechanism when moving upwards.
Further, the rotating strip is rotationally connected with the inner wall of the guide pipe, and the rotating strip is movably sleeved with a torsion spring component which extends to the inner wall of the guide pipe, so that when the pressing block is pressed, the lever mechanism can be utilized to enable the rotating strip to move the wedge strip towards one side far away from the elastic plug rod, and the limitation on the elastic plug rod is relieved.
Further, still include positioning mechanism, positioning mechanism swing joint is in the wall chamber of metal wire section of thick bamboo, positioning mechanism includes V type groove, sink groove, movable chamber, electromagnetic stripe, piston rack, magnetic force gear, butt sponge, the sink groove has been run through to the upper and lower both sides in V type groove, the inner wall of metal wire section of thick bamboo seted up with the movable chamber that the sink groove link up, the middle part fixed mounting in movable chamber has the electromagnetic stripe, the equal sliding connection in left and right sides in movable chamber has the piston rack, the meshing is connected with magnetic force gear between the piston rack, the inner wall fixedly connected with symmetrical two butt sponges of metal wire section of thick bamboo.
Further, the bearing disc is provided with a rotating groove corresponding to the metal wire barrel, and two symmetrical magnetic blocks are buried in the inner wall of the rotating groove, so that the metal wire barrel is kept stable under the action of magnetic attraction of the magnetic blocks, and when the metal wire barrel is forced to rotate, the metal wire barrel can obtain continuously-changed induction current to be communicated with each internal electromagnetic strip.
Further, the V-shaped groove is close to one side of the inner cavity of the metal wire cylinder, and a cone opening is formed in one side of the V-shaped groove, which is close to the inner cavity of the metal wire cylinder, so that when air flows are introduced into the upper side and the lower side of the V-shaped groove, one side of the V-shaped groove, which is far away from the inner cavity of the metal wire cylinder, is discharged, the cone opening can form a negative pressure field according to the Bernoulli principle, and then a reagent tube placed inside the metal wire cylinder can be fixed in a non-contact negative pressure balance mode on two sides, so that slipping and shaking between the reagent tube and the metal wire cylinder during centrifugation of the reagent tube are avoided.
Further, a unidirectional drainage channel is arranged between the movable cavity and the converging groove, and a unidirectional inflow channel is arranged between the movable cavity and the outside, so that when the piston rack rod moves in the movable cavity in a reciprocating manner, outside air can be pumped into and discharged into the converging groove, and air can flow rapidly.
Further, the magnetic gear is elastically connected with the inner wall of the metal wire cylinder, and the magnetic pole of one side, adjacent to the electrified electromagnetic strip, of the magnetic gear is opposite to the magnetic pole of the other side, so that when the current electrified in the electromagnetic strip changes reciprocally, the magnetic gear can deflect reciprocally to drive the piston toothed bars on two sides to move alternately.
Compared with the prior art, the invention has the following beneficial effects:
1. this tumour cell sample pretreatment draws kit, through rotating the tray and make it drive the corresponding metal wire section of thick bamboo that holds the reagent pipe that needs to be centrifuged, by the top back of fixing a position to flexible butt pole, can upwards push the elastic stopper rod, because the elasticity of elastic sub-pipe is far less than the elasticity of flexible butt pole, therefore flexible butt pole is followed the elastic sub-pipe and is moved the biggest to the inner chamber of the cup joint portion of metal wire section of thick bamboo bottom earlier under the atmospheric pressure effect, then can butt to the inner wall of metal wire section of thick bamboo cup joint portion again, because the elastic stopper rod is limited by the wedge when moving upwards, can make the tight connection between flexible butt pole and the metal wire section of thick bamboo, can drive driven gear operation when this time, in order to carry out centrifugal operation to the reagent pipe in the metal wire section of thick bamboo, this design can make a plurality of reagent pipes need not frequently take out to carry out centrifugal operation in corresponding centrifugal equipment, and can be convenient according to the required to centrifugal operation, and when need not centrifugal operation, can demolish with the base of threaded connection between the casing, only make equipment carry out conventional reagent and deposit, thereby effectively promoted the practicality that the kit used.
2. This tumour cell sample preliminary treatment draws kit, in the induction electric current that the automatic acquisition size was kept changing when rotating through the metal wire section of thick bamboo in each electromagnetic stripe, the magnetic force gear that corresponds makes the piston rack bar of activity chamber both sides alternately remove under intermittent type magnetic attraction effect, so that V type groove simultaneously and constantly lets in the air current in upper and lower both sides, one side that the metal wire section of thick bamboo inner chamber was kept away from to the air current V type groove is discharged, and cone mouth department can then form the negative pressure field according to Bernoulli's principle, and then can carry out contactless negative pressure balanced fixed to the reagent pipe that this metal wire section of thick bamboo is inside, only lean on the limiting displacement of butt sponge when avoiding reagent pipe centrifugation, can make skidding rocking between reagent pipe and the metal wire section of thick bamboo, owing to this kind of locate mode has avoided the direct contact with the reagent pipe, consequently can avoid reagent pipe outer wall's location wearing and tearing, and be adapted to the reagent pipe of different specifications, thereby high-efficient location convenience and the security of guaranteeing the reagent pipe.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a front cross-sectional view of the present invention;
FIG. 3 is a front cross-sectional view of the driven gear connecting portion of the present invention;
FIG. 4 is an enlarged view of FIG. 3 at A;
FIG. 5 is an enlarged view at B in FIG. 3;
fig. 6 is a front cross-sectional view of the V-groove connection portion of the present invention.
In the figure: 1. a housing; 2. a receiving tray; 3. a metal wire cylinder; 4. a centrifugal mechanism; 41. a base; 42. a drive gear; 43. a driven gear; 44. a guide pipe; 45. an elastic plug rod; 46. wedge strips; 47. pressing blocks; 48. rotating the strip; 49. an elastic sub-tube; 410. a telescopic supporting rod; 5. a positioning mechanism; 51. a V-shaped groove; 52. a sink groove; 53. a movable cavity; 54. an electromagnetic stripe; 55. a piston rack bar; 56. a magnetic gear; 57. abutting against the sponge.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples of the tumor cell sample pretreatment extraction kit are as follows:
referring to fig. 1-6, a kit for pretreatment and extraction of tumor cell samples comprises a housing 1, wherein a receiving tray 2 is rotatably connected to the inner wall of the housing 1, grooves corresponding to the number of metal wire barrels 3 are uniformly formed in the bottom of the receiving tray 2, and elastic pins extending into the grooves are fixedly connected to the inner wall of the housing 1, so that the receiving tray 2 is conveniently positioned as required.
The metal wire cylinder 3 is rotationally connected to the bearing disc 2, and the bottom of the metal wire cylinder 3 comprises a sleeved part corresponding to the telescopic supporting rod 410, so that the telescopic supporting rod 410 can conveniently extend into the inner cavity of the sleeved part and then can laterally abut against the inner wall of the sleeved part.
The bottom threaded connection of casing 1 has centrifugal mechanism 4, centrifugal mechanism 4 includes base 41, drive gear 42, driven gear 43, lead take over 44, elasticity cock stem 45, wedge 46, press piece 47, change strip 48, elasticity sub-pipe 49, flexible butt pole 410, the middle part inner wall rotation of base 41 is connected with drive gear 42, the left side rotation of base 41 is connected with driven gear 43 (the arrow indication direction is the right side in fig. 2), the fixed grafting in middle part of driven gear 43 has lead take over 44, the bottom slip of lead take over 44 has elasticity cock stem 45, the left wall of elasticity cock stem 45 has seted up the wedge groove that corresponds with wedge 46, thereby can be limited the reset by the wedge 46 that has elastic mechanism when making elasticity cock stem 45 upwards remove.
The left inner wall of the guide pipe 44 is slidably inserted with a wedge strip 46, the left wall of the guide pipe 44 is slidably inserted with a pressing block 47, a rotating strip 48 is movably connected between the pressing block 47 and the wedge strip 46, the rotating strip 48 is rotatably connected with the inner wall of the guide pipe 44, a torsion spring component extending to the inner wall of the guide pipe 44 is movably sleeved on the rotating strip 48, and therefore when the pressing block 47 is pressed, the rotating strip 48 can drive the wedge strip 46 to move towards one side far away from the elastic plug rod 45 by utilizing a lever mechanism so as to contact the limitation of the elastic plug rod 45.
The top sliding connection of guide pipe 44 has elasticity sub-pipe 49, elasticity of elasticity sub-pipe 49 is far less than the elasticity of flexible butt pole 410, but then butt to the inner wall of metal wire section of thick bamboo 3 cup joint portion, because elasticity sub-pipe 49 is limited by wedge 46 when moving up, can make telescopic butt pole 410 and metal wire section of thick bamboo 3 between zonulae occludens, can drive driven gear 43 operation when this time, in order to carry out centrifugal operation to the reagent pipe in the metal wire section of thick bamboo 3, can push up elasticity cock stem 45 upwards, because elasticity sub-pipe 49's elasticity is far less than the elasticity of flexible butt pole 410, consequently, flexible butt pole 410 moves up the inner wall of maximum distance to the cup joint portion of metal wire section of thick bamboo 3 under the pneumatic action, then can abut against to the metal wire section of thick bamboo 3 cup joint portion, because elasticity cock stem 45 is limited by wedge 46 when moving up, can make the tight connection between flexible butt pole 410 and the metal wire section of thick bamboo 3, can drive driven gear 43 operation when so as to carry out centrifugal operation to the reagent pipe in the metal wire section of thick bamboo 3, this design can make a plurality of reagent pipes need not frequently take out centrifugal operation to corresponding centrifugal equipment, and can remove the reagent pipe and carry out centrifugal operation according to, and can be used for the normal operation, and can be convenient and high-efficient can store the reagent box 1, can be used to carry out the convenient and can be connected with the convenient and has the performance to install and can use according to the test box.
The positioning mechanism 5 is movably connected with the wall cavity of the metal wire cylinder 3, the positioning mechanism 5 comprises a V-shaped groove 51, a converging groove 52, a movable cavity 53, an electromagnetic strip 54, a piston toothed bar 55, a magnetic gear 56 and a butt sponge 57, the converging groove 52 is formed in a penetrating manner on the upper side and the lower side of the V-shaped groove 51, one side of the V-shaped groove 51, which is close to the inner cavity of the metal wire cylinder 3, is provided with a cone opening, so that when the upper side and the lower side of the V-shaped groove 51 are simultaneously filled with air flow, the air flow is discharged from one side, which is far away from the inner cavity of the metal wire cylinder 3, of the V-shaped groove 51, the cone opening part forms a negative pressure field according to Bernoulli principle, and then a reagent tube placed inside the metal wire cylinder 3 can be fixed in a non-contact negative pressure balance manner on two sides, and slipping and shaking between the reagent tube and the metal wire cylinder 3 during centrifugation can be avoided.
The inner wall of the metal wire cylinder 3 is provided with a movable cavity 53 communicated with the converging groove 52, a unidirectional drainage channel is arranged between the movable cavity 53 and the converging groove 52, and a unidirectional inflow channel is arranged between the movable cavity 53 and the outside, so that when the piston toothed bar 55 reciprocates in the movable cavity 53, outside air can be pumped into and discharged into the converging groove 52, and air can flow rapidly.
The middle part of the movable cavity 53 is fixedly provided with an electromagnetic strip 54, the receiving disc 2 is provided with a rotary groove corresponding to the metal wire barrel 3, and the inner wall of the rotary groove is embedded with two symmetrical magnetic blocks, so that the metal wire barrel 3 is kept stable under the action of magnetic attraction of the magnetic blocks, and when the metal wire barrel 3 is forced to rotate, the metal wire barrel 3 can obtain continuously-changed induction current to be communicated with each electromagnetic strip 54 inside.
The left side and the right side of the movable cavity 53 are both slidably connected with piston toothed bars 55, magnetic gears 56 are connected between the piston toothed bars 55 in a meshed manner, the magnetic gears 56 are elastically connected with the inner wall of the metal wire cylinder 3, and magnetic poles of one side, adjacent to the electrified electromagnetic strip 54, of the magnetic gears 56 are opposite, so that when current introduced into the electromagnetic strip 54 changes reciprocally, the magnetic gears 56 can deflect reciprocally to drive the piston toothed bars 55 on two sides to move alternately, and two symmetrical abutting sponges 57 are fixedly connected with the inner wall of the metal wire cylinder 3.
The induction current with the continuously-changing size is automatically obtained when the metal wire cylinder 3 rotates and is transmitted to each electromagnetic strip 54, the corresponding magnetic gears 56 enable the piston toothed bars 55 on two sides of the movable cavity 53 to move in a staggered mode under the intermittent magnetic attraction effect, so that the V-shaped grooves 51 simultaneously and continuously and rapidly feed in air current on the upper side and the lower side, one side, far away from the inner cavity of the metal wire cylinder 3, of the air current flow through the V-shaped grooves 51 is discharged, the cone opening part can form the negative pressure field according to the Bernoulli principle, and then the reagent tubes placed inside the metal wire cylinder 3 can be subjected to non-contact negative pressure balance fixation on two sides, so that slipping and shaking between the reagent tubes and the metal wire cylinder 3 are avoided due to the limiting effect of abutting the sponge 57 when the reagent tubes are centrifuged, positioning abrasion of the outer walls of the reagent tubes can be avoided due to the positioning mode, and the reagent tubes with different specifications are adapted to the reagent tubes are efficiently ensured, and the positioning convenience and safety of the reagent tubes are efficiently ensured.
Working principle: when in use, after the reagent tube is positioned in the metal wire tube 3, the supporting disk 2 is rotated to adjust the reagent tube to be centrifugally operated to the top of the telescopic supporting rod 410, the supporting disk 2 is fixed by utilizing the clamping mechanism of the elastic pin and the groove at the bottom of the supporting disk 2, then the elastic plug rod 45 is pushed upwards, the elastic sub-tube 49 moves upwards to the inner cavity of the sleeving part at the bottom of the metal wire tube 3 under the action of air pressure because the elastic force of the elastic sub-tube 49 is far smaller than the elastic force of the telescopic supporting rod 410, then the telescopic supporting rod 410 which moves upwards along with the elastic sub-tube 49 is abutted to the inner wall of the sleeving part of the metal wire tube 3 along with the continuous extrusion of air, and after the elastic plug rod 45 is pushed upwards by the corresponding distance because the elastic plug rod 45 is continuously limited by the wedge strip 46 with the elastic mechanism, the telescopic support rod 410 can be tightly connected with the metal wire cylinder 3, the driving gear 42 can be driven by an external drive to drive the driven gear 43 to operate at the moment so as to perform centrifugal operation on the reagent tubes in the corresponding metal wire cylinder 3, the design can ensure that a plurality of reagent tubes do not need to be frequently taken out to corresponding centrifugal equipment to perform centrifugal operation, the designated reagent tubes can be conveniently and rapidly centrifuged according to the needs, when the centrifugal operation is not needed, the base 41 in threaded connection with the shell 1 can be dismantled, the equipment only performs the conventional reagent storage function, thereby effectively improving the practical performance of the use of the reagent kit, in addition, when the metal wire cylinder 3 rotates, the magnetic induction wire of the automatic cutting magnetic block obtains the induction current with the continuous change of the size, the corresponding electromagnetic strip 54 intermittently magnetically attracts the magnetic gear 56, the piston toothed bars 55 at two sides of the movable cavity 53 are alternately moved, so that the external air is respectively sucked into the two sides of the movable cavity 53 and then discharged into the confluence groove 52 to flow rapidly, and when the air flows are simultaneously and continuously introduced into the upper side and the lower side of the V-shaped groove 51, the air flows are discharged from one side of the inner cavity of the metal wire cylinder 3 through the V-shaped groove 51, and the cone opening can form the negative pressure field according to the Bernoulli principle, so that the reagent tube placed in the metal wire cylinder 3 can be fixed in a non-contact negative pressure balance manner on the two sides, the reagent tube is prevented from sliding and shaking between the reagent tube and the metal wire cylinder 3 due to the limiting action of only abutting the sponge 57 when the reagent tube is centrifuged, the positioning abrasion of the outer wall of the reagent tube can be avoided due to the direct contact between the reagent tube and the reagent tube is adapted to the reagent tubes with different specifications, and the positioning convenience and safety of the reagent tube are effectively ensured.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. The utility model provides a tumour cell sample preliminary treatment extraction kit, includes casing (1), its characterized in that: the inner wall of the shell (1) is rotationally connected with a receiving disc (2), the receiving disc (2) is rotationally connected with a metal wire cylinder (3), the bottom of the shell (1) is in threaded connection with a centrifugal mechanism (4), the centrifugal mechanism (4) comprises a base (41), a driving gear (42), a driven gear (43), a guide pipe (44), an elastic plug rod (45), a wedge strip (46), a pressing block (47), a rotating strip (48), an elastic sub-pipe (49) and a telescopic supporting rod (410), the middle inner wall of the base (41) is rotationally connected with the driving gear (42), the left side of the base (41) is rotationally connected with the driven gear (43), the middle part of the driven gear (43) is fixedly connected with a guide pipe (44), the bottom of the guide pipe (44) is slidingly connected with the elastic plug rod (45), the left side inner wall of the guide pipe (44) is slidingly connected with a wedge strip (46), the left wall of the guide pipe (44) is slidingly connected with a pressing block (47), the pressing block (47) is movably connected with the wedge strip (46), the elastic sub-pipe (49) is slidingly connected with the top of the elastic sub-pipe (49), the elastic force of the elastic sub-tube (49) is far smaller than that of the telescopic supporting rod (410);
a wedge groove corresponding to the wedge strip (46) is formed in the left wall of the elastic plug rod (45);
the rotating strip (48) is rotationally connected with the inner wall of the guide pipe (44), and a torsion spring component extending to the inner wall of the guide pipe (44) is movably sleeved on the rotating strip (48);
the positioning mechanism (5) is movably connected with a wall cavity of the metal wire cylinder (3), the positioning mechanism (5) comprises a V-shaped groove (51), a converging groove (52), a movable cavity (53), an electromagnetic bar (54), a piston toothed bar (55), a magnetic gear (56) and a butt sponge (57), converging grooves (52) are formed in the upper side and the lower side of the V-shaped groove (51), the movable cavity (53) communicated with the converging groove (52) is formed in the inner wall of the metal wire cylinder (3), an electromagnetic bar (54) is fixedly arranged in the middle of the movable cavity (53), the piston toothed bar (55) is connected to the left side and the right side of the movable cavity (53) in a sliding mode, the magnetic gear (56) is connected between the piston toothed bars (55) in a meshed mode, and two symmetrical butt sponges (57) are fixedly connected to the inner wall of the metal wire cylinder (3).
The bottom of the metal wire cylinder (3) comprises a sleeving part corresponding to the telescopic supporting rod (410);
a rotary groove corresponding to the metal wire cylinder (3) is formed in the receiving disc (2), and two symmetrical magnetic blocks are buried in the inner wall of the rotary groove;
the magnetic gear (56) is elastically connected with the inner wall of the metal wire cylinder (3), and the magnetic gear (56) is opposite to one side of the electrified electromagnetic strip (54) adjacent to the magnetic pole.
2. The tumor cell sample pretreatment extraction kit according to claim 1, wherein: grooves corresponding to the metal wire drums (3) in number are uniformly formed in the bottom of the receiving disc (2), and elastic pins extending into the grooves are fixedly connected to the inner wall of the shell (1).
3. The tumor cell sample pretreatment extraction kit according to claim 1, wherein: and one side of the V-shaped groove (51) close to the inner cavity of the metal wire cylinder (3) is provided with a cone opening.
4. The tumor cell sample pretreatment extraction kit according to claim 1, wherein: a unidirectional drainage channel is arranged between the movable cavity (53) and the converging groove (52), and a unidirectional inflow channel is arranged between the movable cavity (53) and the outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111567819.2A CN114308410B (en) | 2021-12-20 | 2021-12-20 | Tumor cell sample pretreatment extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111567819.2A CN114308410B (en) | 2021-12-20 | 2021-12-20 | Tumor cell sample pretreatment extraction kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114308410A CN114308410A (en) | 2022-04-12 |
| CN114308410B true CN114308410B (en) | 2023-11-10 |
Family
ID=81055040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111567819.2A Active CN114308410B (en) | 2021-12-20 | 2021-12-20 | Tumor cell sample pretreatment extraction kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114308410B (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2908054A1 (en) * | 2006-11-07 | 2008-05-09 | Partus Technologies Sa | Non-miscible liquid-liquid separating method for disk/monoblock type cylindrical rotor, involves adjusting rotor rotation direction and driving direction to oppositely set driving direction and circuit's content driving direction |
| JP2009154038A (en) * | 2007-12-25 | 2009-07-16 | Hitachi Koki Co Ltd | Cell lavage centrifuge |
| WO2011136606A2 (en) * | 2010-04-29 | 2011-11-03 | Shin Jun-Ho | Kit and method for extracting buffy coats |
| CN108325762A (en) * | 2018-03-13 | 2018-07-27 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse and bio-separation device |
| CN108554656A (en) * | 2018-03-13 | 2018-09-21 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse separation equipment |
| CN211070483U (en) * | 2019-09-28 | 2020-07-24 | 江苏鹏程实验器材有限公司 | Cell centrifugal equipment for medical science experiments |
| CN211678270U (en) * | 2020-02-25 | 2020-10-16 | 中南大学湘雅三医院 | Leukemia lymphocyte separation and extraction device |
| CN111979089A (en) * | 2019-05-23 | 2020-11-24 | 比欧泰克生物技术服务(北京)有限公司 | Large-volume stem cell exosome separation and concentration device and concentration process |
| CN112354698A (en) * | 2020-12-04 | 2021-02-12 | 广州金智医疗器械有限公司 | Blood sample centrifuge capable of automatically separating and subpackaging |
| CN213435071U (en) * | 2020-09-06 | 2021-06-15 | 博源润生医药（杭州）有限公司 | Efficient biological cell centrifugal device |
| CN113083520A (en) * | 2021-03-16 | 2021-07-09 | 韩宝云 | Blood centrifuge is used in inspection |
| CN214361330U (en) * | 2020-12-01 | 2021-10-08 | 杭州康金来技术有限公司 | Automatic nucleic acid extraction device |
| CN113771097A (en) * | 2021-11-15 | 2021-12-10 | 诺伯特智能装备（山东）有限公司 | Industrial robot signal device based on intelligence is made |
| CN113786934A (en) * | 2021-09-17 | 2021-12-14 | 郑州大学第一附属医院 | Kit for extracting total RNA from blood and use method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3803078B2 (en) * | 2002-09-20 | 2006-08-02 | 独立行政法人科学技術振興機構 | Hematology analyzer and plasma separation method |
| KR100606264B1 (en) * | 2004-11-19 | 2006-07-31 | 주식회사 한랩 | centrifugal apparatus of automatic balancing type by fluid compensation |
| CN107213996B (en) * | 2017-04-07 | 2018-08-24 | 郑肇恒 | A kind of high speed breakage-proof pollution refrigerated centrifuge for extracting cell |
-
2021
- 2021-12-20 CN CN202111567819.2A patent/CN114308410B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2908054A1 (en) * | 2006-11-07 | 2008-05-09 | Partus Technologies Sa | Non-miscible liquid-liquid separating method for disk/monoblock type cylindrical rotor, involves adjusting rotor rotation direction and driving direction to oppositely set driving direction and circuit's content driving direction |
| JP2009154038A (en) * | 2007-12-25 | 2009-07-16 | Hitachi Koki Co Ltd | Cell lavage centrifuge |
| WO2011136606A2 (en) * | 2010-04-29 | 2011-11-03 | Shin Jun-Ho | Kit and method for extracting buffy coats |
| CN108325762A (en) * | 2018-03-13 | 2018-07-27 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse and bio-separation device |
| CN108554656A (en) * | 2018-03-13 | 2018-09-21 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse separation equipment |
| CN111979089A (en) * | 2019-05-23 | 2020-11-24 | 比欧泰克生物技术服务(北京)有限公司 | Large-volume stem cell exosome separation and concentration device and concentration process |
| CN211070483U (en) * | 2019-09-28 | 2020-07-24 | 江苏鹏程实验器材有限公司 | Cell centrifugal equipment for medical science experiments |
| CN211678270U (en) * | 2020-02-25 | 2020-10-16 | 中南大学湘雅三医院 | Leukemia lymphocyte separation and extraction device |
| CN213435071U (en) * | 2020-09-06 | 2021-06-15 | 博源润生医药（杭州）有限公司 | Efficient biological cell centrifugal device |
| CN214361330U (en) * | 2020-12-01 | 2021-10-08 | 杭州康金来技术有限公司 | Automatic nucleic acid extraction device |
| CN112354698A (en) * | 2020-12-04 | 2021-02-12 | 广州金智医疗器械有限公司 | Blood sample centrifuge capable of automatically separating and subpackaging |
| CN113083520A (en) * | 2021-03-16 | 2021-07-09 | 韩宝云 | Blood centrifuge is used in inspection |
| CN113786934A (en) * | 2021-09-17 | 2021-12-14 | 郑州大学第一附属医院 | Kit for extracting total RNA from blood and use method thereof |
| CN113771097A (en) * | 2021-11-15 | 2021-12-10 | 诺伯特智能装备（山东）有限公司 | Industrial robot signal device based on intelligence is made |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114308410A (en) | 2022-04-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106884782B (en) | peristaltic pump | |
| CN114308410B (en) | Tumor cell sample pretreatment extraction kit | |
| CN108761100B (en) | Automatic cross matching test method for unknown blood type | |
| CN110261174B (en) | Sampling device for water quality detection and using method thereof | |
| CN213580266U (en) | Food detects uses liquid food sampling device | |
| CN113514654A (en) | Storage device for saliva detection and collection and use method | |
| CN211292150U (en) | Handheld crop trace element content sample extractor convenient to store | |
| CN112595553A (en) | Liquid sampling device for biological extraction of related water body by utilizing water pressure | |
| CN218646649U (en) | Food detects uses quick sampler | |
| CN217930695U (en) | Vacuum is pushed down frame electronic product and is detected smelting tool | |
| CN206949381U (en) | A kind of soft plane unhairing machine of single shaft | |
| CN115931463A (en) | Sampling device of edible fungus constant-temperature incubator and use method | |
| CN212975165U (en) | Protein preservation kit convenient to sample | |
| CN210965180U (en) | Centrifuge tube for manufacturing cell wax block | |
| CN217786648U (en) | Lubricating oil detection sampling device | |
| CN211967007U (en) | Automatic polishing equipment for machining of circulating pump shielding cover | |
| CN209247491U (en) | A kind of detection device for absorbent article | |
| CN111040929A (en) | Gene capturing kit for capturing related genes of hematopathy | |
| CN209821246U (en) | Tool for disassembling terminal | |
| CN219641686U (en) | Sampling detection device is used in tealeaves production | |
| CN108562543B (en) | A kind of routine biochemistry analysis instrument | |
| CN218035878U (en) | Water resource detects uses sampler | |
| CN219948934U (en) | Preparation kit for porcine virus antibody | |
| CN213689041U (en) | Quantitative sampling and reagent adding device for medical detection | |
| CN219786383U (en) | Corner processing equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |